WO2012027366A3 - Acoustic waves in microfluidics - Google Patents

Acoustic waves in microfluidics Download PDF

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Publication number
WO2012027366A3
WO2012027366A3 PCT/US2011/048804 US2011048804W WO2012027366A3 WO 2012027366 A3 WO2012027366 A3 WO 2012027366A3 US 2011048804 W US2011048804 W US 2011048804W WO 2012027366 A3 WO2012027366 A3 WO 2012027366A3
Authority
WO
WIPO (PCT)
Prior art keywords
microfluidic
droplets
acoustic waves
surface acoustic
piezoelectric substrate
Prior art date
Application number
PCT/US2011/048804
Other languages
French (fr)
Other versions
WO2012027366A2 (en
Inventor
David A. Weitz
Thomas Franke
Achim Wixforth
Lothar Schmid
Jeremy Agresti
Adam R. Abate
Original Assignee
President And Fellows Of Harvard College
Universität Augsburg
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by President And Fellows Of Harvard College, Universität Augsburg filed Critical President And Fellows Of Harvard College
Priority to US13/818,146 priority Critical patent/US9695390B2/en
Priority to EP11820522.8A priority patent/EP2608878A4/en
Publication of WO2012027366A2 publication Critical patent/WO2012027366A2/en
Publication of WO2012027366A3 publication Critical patent/WO2012027366A3/en
Priority to US15/604,085 priority patent/US10570361B2/en
Priority to US16/238,467 priority patent/US11229911B2/en
Priority to US17/550,612 priority patent/US20220212194A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502776—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for focusing or laminating flows
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F17—STORING OR DISTRIBUTING GASES OR LIQUIDS
    • F17D—PIPE-LINE SYSTEMS; PIPE-LINES
    • F17D3/00—Arrangements for supervising or controlling working operations
    • F17D3/01—Arrangements for supervising or controlling working operations for controlling, signalling, or supervising the conveyance of a product
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0636—Focussing flows, e.g. to laminate flows
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652—Sorting or classification of particles or molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0421—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electrophoretic flow
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0433—Moving fluids with specific forces or mechanical means specific forces vibrational forces
    • B01L2400/0436—Moving fluids with specific forces or mechanical means specific forces vibrational forces acoustic forces, e.g. surface acoustic waves [SAW]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0493—Specific techniques used
    • B01L2400/0496—Travelling waves, e.g. in combination with electrical or acoustic forces
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1028—Sorting particles
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T137/00—Fluid handling
    • Y10T137/0318—Processes
    • Y10T137/0391—Affecting flow by the addition of material or energy

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Fluid Mechanics (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Mechanical Engineering (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Various aspects of the present invention relate to the control and manipulation of fluidic species, for example, in microfluidic systems. In one set of embodiments, droplets may be sorted using surface acoustic waves. The droplets may contain cells or other species. In some cases, the surface acoustic waves may be created using a surface acoustic wave generator such as an interdigitated transducer, and/or a material such as a piezoelectric substrate. The piezoelectric substrate may be isolated from the microfluidic substrate except at or proximate the location where the droplets are sorted, e.g., into first or second microfluidic channels. At such locations, the microfluidic substrate may be coupled to the piezoelectric substrate (or other material) by one or more coupling regions. In some cases, relatively high sorting rates may be achieved, e.g., at rates of at least about 1,000 Hz, at least about 10,000 Hz, or at least about 100,000 Hz, and in some embodiments, with high cell viability after sorting.
PCT/US2011/048804 2010-08-23 2011-08-23 Acoustic waves in microfluidics WO2012027366A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
US13/818,146 US9695390B2 (en) 2010-08-23 2011-08-23 Acoustic waves in microfluidics
EP11820522.8A EP2608878A4 (en) 2010-08-23 2011-08-23 Acoustic waves in microfluidics
US15/604,085 US10570361B2 (en) 2010-08-23 2017-05-24 Acoustic waves in microfluidics
US16/238,467 US11229911B2 (en) 2010-08-23 2019-01-02 Acoustic waves in microfluidics
US17/550,612 US20220212194A1 (en) 2010-08-23 2021-12-14 Acoustic waves in microfluidics

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US37613710P 2010-08-23 2010-08-23
US61/376,137 2010-08-23

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US13/818,146 A-371-Of-International US9695390B2 (en) 2010-08-23 2011-08-23 Acoustic waves in microfluidics
US15/604,085 Continuation US10570361B2 (en) 2010-08-23 2017-05-24 Acoustic waves in microfluidics

Publications (2)

Publication Number Publication Date
WO2012027366A2 WO2012027366A2 (en) 2012-03-01
WO2012027366A3 true WO2012027366A3 (en) 2012-08-16

Family

ID=45724018

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2011/048804 WO2012027366A2 (en) 2010-08-23 2011-08-23 Acoustic waves in microfluidics

Country Status (3)

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US (4) US9695390B2 (en)
EP (1) EP2608878A4 (en)
WO (1) WO2012027366A2 (en)

Families Citing this family (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0415913B1 (en) 2003-10-30 2017-09-26 Cytonome/St, Llc STRUCTURE AND FLOW SYSTEM FOR SUSPENDING A PARTICLE AND METHOD FOR WRAPPING THAT PARTICLE ON AT LEAST TWO SIDES BY A INVOLVING FLUID
GB2471522B (en) * 2009-07-03 2014-01-08 Cambridge Entpr Ltd Microfluidic devices
GB0914762D0 (en) 2009-08-24 2009-09-30 Univ Glasgow Fluidics apparatus and fluidics substrate
WO2012027366A2 (en) 2010-08-23 2012-03-01 President And Fellows Of Harvard College Acoustic waves in microfluidics
GB201103211D0 (en) * 2011-02-24 2011-04-13 Univ Glasgow Fluidics apparatus, use of fluidics apparatus and process for the manufacture of fluidics apparatus
US10080997B2 (en) * 2012-03-16 2018-09-25 Versitech Limited System and method for generation of emulsions with low interfacial tension and measuring frequency vibrations in the system
WO2014004630A1 (en) * 2012-06-27 2014-01-03 President And Fellows Of Harvard College Control of entities such as droplets and cells using acoustic waves
CN104968417B (en) * 2012-08-01 2017-08-18 宾夕法尼亚州立大学研究基金会 The high efficiency separation and operation of particle and cell
US10161007B2 (en) 2012-08-13 2018-12-25 The Regents Of The University Of California Methods and systems for detecting biological components
US20150298157A1 (en) * 2012-10-26 2015-10-22 President And Fellows Of Harvard College Systems and methods for droplet production and manipulation using acoustic waves
WO2014138715A1 (en) * 2013-03-08 2014-09-12 Duke University Devices, systems, and methods for acoustically -enhanced magnetophoresis
NZ711384A (en) 2013-03-14 2018-06-29 Cytonome St Llc Hydrodynamic focusing apparatus and methods
US10052431B2 (en) 2014-06-09 2018-08-21 Ascent Bio-Nano Technologies, Inc. System for manipulation and sorting of particles
WO2015200616A1 (en) * 2014-06-26 2015-12-30 President And Fellows Of Harvard College Fluid injection using acoustic waves
DE102014222680A1 (en) * 2014-11-06 2016-05-12 Leibniz-Institut Für Festkörper- Und Werkstoffforschung Dresden E.V. LIQUID SCREENING DEVICE AND METHOD FOR THE PRODUCTION THEREOF
EP3017868A1 (en) 2014-11-10 2016-05-11 Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie - Hans-Knöll-Institut - Device and method for extracting individual picoliter droplets from microfluidic emulsions for further analysis and scale-up
GB201420061D0 (en) * 2014-11-11 2014-12-24 Univ Glasgow Nebulisation of liquids
EP4112744A1 (en) 2015-02-04 2023-01-04 The Regents of the University of California Sequencing of nucleic acids via barcoding in discrete entities
EP3253910B1 (en) * 2015-02-04 2021-07-14 The Regents of The University of California Multiple-emulsion nucleic acid amplification
FR3032974B1 (en) 2015-02-19 2023-01-13 Commissariat Energie Atomique DEVICE FOR MANIPULATING BIOLOGICAL CELLS BY MEANS OF A VIBRATING SUPPORT
WO2016149639A1 (en) * 2015-03-19 2016-09-22 The Board Of Trustees Of The Leland Stanford Junior University Devices and methods for high-throughput single cell and biomolecule analysis and retrieval in a microfluidic chip
WO2016191804A1 (en) * 2015-06-02 2016-12-08 Monash University Controlled droplet production and manipulation from a plug using surface acoustic waves
KR101954639B1 (en) * 2015-06-25 2019-03-07 사이토놈/에스티, 엘엘씨 Microfluidic devices and systems using acoustic manipulation
WO2017004676A1 (en) * 2015-07-09 2017-01-12 Monash University On-demand single cell encapsulation using a localised acoustic field
DK3341116T3 (en) 2015-08-27 2022-05-02 Harvard College SORTING PROCEDURE USING ACOUSTIC WAVES
CN108495712A (en) * 2015-11-23 2018-09-04 伯克利之光生命科技公司 Generated in-situ microfluid isolation structure, kit and its application method
HUE061715T2 (en) * 2015-12-23 2023-08-28 Siemens Healthcare Gmbh Flow cell for analyzing particles in a liquid to be examined, method and use
WO2017176211A1 (en) * 2016-04-06 2017-10-12 Singapore University Of Technology And Design Particle manipulation
US11607658B2 (en) 2016-07-08 2023-03-21 President And Fellows Of Harvard College Formation of colloids or gels within droplets
WO2018027282A1 (en) * 2016-08-12 2018-02-15 Royal Melbourne Institute Of Technology Arrayable acoustic actuators for microfluidics
WO2018048876A1 (en) * 2016-09-06 2018-03-15 Lariat Biosciences, Inc. Microfluidic device
WO2018058113A2 (en) * 2016-09-26 2018-03-29 Duke University Systems and methods for analysis of liquids by covered fluidic channels integrated onto sensor platforms
WO2018148715A1 (en) * 2017-02-13 2018-08-16 The Regents Of The University Of California Device and method for intracellular delivery of biomolecular cargo via acoustic wave exposure
LT3375889T (en) 2017-03-17 2020-06-25 Hifibio Sas Single cell analysis
US10969324B2 (en) 2017-08-16 2021-04-06 Washington University Synthesis, post-modification and separation of biologics using acoustically confined substrates
EP3676005B1 (en) * 2017-08-31 2024-09-25 AcouSort AB Methods and devices for acoustophoretic operations in polymer chips
CN108845026B (en) * 2018-03-07 2020-11-24 中国科学院深圳先进技术研究院 Acoustic surface wave micro-fluidic chip and micron-sized acoustic focal region forming method
US20200114323A1 (en) * 2018-10-12 2020-04-16 Feistel Holding Corp. Systems and methods for treating and conditioning small volume liquid samples
CN109731621B (en) * 2019-01-02 2020-07-24 京东方科技集团股份有限公司 Microfluidic substrate, preparation method thereof and microfluidic panel
EP3711668A1 (en) * 2019-03-18 2020-09-23 Koninklijke Philips N.V. A sweat sensing system
US11573205B2 (en) * 2019-04-12 2023-02-07 Awe Technologies Llc Multimodal characterization of a fluid using a single droplet
US11701658B2 (en) 2019-08-09 2023-07-18 President And Fellows Of Harvard College Systems and methods for microfluidic particle selection, encapsulation, and injection using surface acoustic waves
EP3796212B1 (en) 2019-09-23 2024-06-26 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Device for image-based cell classification, method therefor and use thereof
JP7361228B2 (en) * 2020-04-28 2023-10-13 シーメンス・ヘルスケア・ダイアグノスティックス・インコーポレイテッド Acoustophoretic lysis devices and methods
DE102021100692A1 (en) 2021-01-14 2021-07-08 Dionex Softron Gmbh SAW-based flow sensor
CN113996362B (en) * 2021-12-03 2023-02-03 郑州轻工业大学 Liquid drop fusion microfluidic device and method based on focusing surface acoustic wave regulation

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100139377A1 (en) * 2008-12-05 2010-06-10 The Penn State Reserch Foundation Particle focusing within a microfluidic device using surface acoustic waves

Family Cites Families (71)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT389235B (en) 1987-05-19 1989-11-10 Stuckart Wolfgang METHOD FOR CLEANING LIQUIDS BY MEANS OF ULTRASOUND AND DEVICES FOR CARRYING OUT THIS METHOD
US5853686A (en) 1993-08-10 1998-12-29 State Of Oregon Acting By And Through The Oregon State Board Of Higher Education On Behalf Of The University Of Oregon Calcium carbonates of altered crystal habit or morphology and methods for producing same
US5512131A (en) 1993-10-04 1996-04-30 President And Fellows Of Harvard College Formation of microstamped patterns on surfaces and derivative articles
JP3286463B2 (en) 1994-05-10 2002-05-27 ポーラ化成工業株式会社 Organic-inorganic composite pigment and method for producing the same
WO1996029629A2 (en) 1995-03-01 1996-09-26 President And Fellows Of Harvard College Microcontact printing on surfaces and derivative articles
US5688405A (en) 1996-02-28 1997-11-18 The United States Of America As Represented By The Secretary Of The Navy Method and apparatus for separating particulate matter from a fluid
US5942425A (en) 1996-03-12 1999-08-24 Walters; Adriann H. Method to access nucleic acids from cells
US6355198B1 (en) 1996-03-15 2002-03-12 President And Fellows Of Harvard College Method of forming articles including waveguides via capillary micromolding and microtransfer molding
JPH1082723A (en) 1996-09-06 1998-03-31 Hitachi Ltd Microparticle processor
US6948843B2 (en) 1998-10-28 2005-09-27 Covaris, Inc. Method and apparatus for acoustically controlling liquid solutions in microfluidic devices
RU2189961C2 (en) 1999-07-19 2002-09-27 Карапетян Гарегин Оганесович Method of immobilization of physiologically active compounds
US6645432B1 (en) 2000-05-25 2003-11-11 President & Fellows Of Harvard College Microfluidic systems including three-dimensionally arrayed channel networks
US6777245B2 (en) * 2000-06-09 2004-08-17 Advalytix Ag Process for manipulation of small quantities of matter
SE522801C2 (en) * 2001-03-09 2004-03-09 Erysave Ab Apparatus for separating suspended particles from an ultrasonic fluid and method for such separation
JP2002348234A (en) 2001-05-28 2002-12-04 Purotekku:Kk Drug-encapsulating inorganic microparticle, manufacturing method thereof and preparation comprising drug-encapsulating inorganic microparticle
JP2006507921A (en) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ Method and apparatus for fluid dispersion
AU2003263986A1 (en) 2002-08-01 2004-02-23 Arizona Board Of Regents Dynamically formed rotors for lock-in amplifier detection
JP3898103B2 (en) 2002-08-26 2007-03-28 独立行政法人科学技術振興機構 Cell analysis separator
DE10337016B4 (en) 2002-09-11 2014-06-26 Merck Patent Gmbh Liquid crystalline medium with high birefringence and its use
JP5285201B2 (en) 2002-09-25 2013-09-11 株式会社ナノエッグ Retinoic acid nanocapsule
JP2004168701A (en) 2002-11-19 2004-06-17 Osaka Industrial Promotion Organization Inorganic ionic molecular crystal
CA2784762A1 (en) 2003-04-10 2004-10-28 President And Fellows Of Harvard College Formation and control of fluidic species
CN1842368B (en) 2003-08-27 2014-05-28 哈佛大学 Electronic control of fluidic species
GB0322590D0 (en) 2003-09-26 2003-10-29 Xaar Technology Ltd Droplet deposition apparatus
EP1674093A4 (en) 2003-10-15 2009-12-09 Nanoegg Res Lab Inc Method of controlling particle size of retinoic acid nanoparticles coated with polyvalent metal inorganic salt and nanoparticles obtained by the controlling method
US20060024206A1 (en) 2004-07-29 2006-02-02 Sinha Naveen N Non-invasive acoustic technique for mixing and segregation of fluid suspensions in microfluidic applications
US20070054119A1 (en) 2005-03-04 2007-03-08 Piotr Garstecki Systems and methods of forming particles
US9039273B2 (en) 2005-03-04 2015-05-26 President And Fellows Of Harvard College Method and apparatus for forming multiple emulsions
JP5702909B2 (en) 2005-03-23 2015-04-15 ヴェロシス インコーポレイテッド Surface functional structure in microprocess technology
WO2006114895A1 (en) 2005-04-21 2006-11-02 Kabushiki Kaisha Nihon Micronics Electrical connection device
US7918244B2 (en) 2005-05-02 2011-04-05 Massachusetts Institute Of Technology Microfluidic bubble logic devices
US7942568B1 (en) * 2005-06-17 2011-05-17 Sandia Corporation Active micromixer using surface acoustic wave streaming
DE602005016736D1 (en) * 2005-07-07 2009-10-29 Fraunhofer Ges Forschung TION OF PARTICLES, CELLS AND VIRUSES
US7964078B2 (en) * 2005-11-07 2011-06-21 The Regents Of The University Of California Microfluidic device for cell and particle separation
WO2007128045A1 (en) 2006-05-02 2007-11-15 Monash University Microfluidic systems using surface acoustic energy and method of use thereof
US7794136B2 (en) 2006-05-09 2010-09-14 National Tsing Hua University Twin-vortex micromixer for enforced mass exchange
EP2041084B1 (en) 2006-06-08 2013-11-06 UCB Pharma, S.A. Co-crystals of pyrrolidinones
WO2008000042A1 (en) 2006-06-30 2008-01-03 Iceutica Pty Ltd Methods for the preparation of biologically active compounds in nanoparticulate form
EP1905427B1 (en) 2006-09-28 2010-11-24 Losan Pharma GmbH Rapidly solubilising formulation of non-steroidal anti-inflammatory drugs
BRPI0716275A2 (en) 2006-12-12 2015-01-20 Firmenich & Cie ACTIVE INGREDIENT DISTRIBUTION SYSTEM WITH A MORPHAL SALT AS CARRIER
WO2008153763A1 (en) 2007-05-25 2008-12-18 Massachusetts Institute Of Technology Three dimensional single-chamber fuel cells
PL2014280T3 (en) 2007-07-13 2015-04-30 Cc Ery Gmbh Micro-particles, blood-substitute and method for forming same
CN101099727A (en) 2007-07-20 2008-01-09 浙江大学 Method for preparing microcapsule having function of specific connecting with tumor cell
ITTO20070554A1 (en) * 2007-07-26 2009-01-27 Fond Istituto Italiano Di Tec DEVICE FOR THE CONTROL OF THE MOTION OF FLUIDS IN MICRO OR NANOCANALS BY SURFACE ACOUSTIC WAVES.
GB0724478D0 (en) 2007-12-14 2008-01-30 Karobio Ab Pharmaceutical compositions
EP2274593A4 (en) * 2008-04-23 2014-07-23 Société De Commercialisation Des Produits De La Rech Appliquée Socpra Sciences Et Génie S E C Integrated shear-vertical surface acoustic wave and surface plasmon resonance sensing device and method
US8387803B2 (en) 2008-08-26 2013-03-05 Ge Healthcare Bio-Sciences Ab Particle sorting
WO2010056984A2 (en) 2008-11-14 2010-05-20 The Regents Of The University Of Michigan Acoustical fluid control mechanism
JP2010252785A (en) 2009-03-31 2010-11-11 Kanagawa Acad Of Sci & Technol Device for concentrating and separating cell
AU2010239081C1 (en) 2009-04-24 2014-11-06 Iceutica Pty Ltd A novel formulation of indomethacin
US8356714B2 (en) 2009-06-02 2013-01-22 Georgia Tech Research Corporation Microfluidic device for separation of particles
GB0914762D0 (en) 2009-08-24 2009-09-30 Univ Glasgow Fluidics apparatus and fluidics substrate
JPWO2011030519A1 (en) * 2009-09-11 2013-02-04 パナソニック株式会社 Elastic wave element and acoustic wave element sensor
FR2950544B1 (en) 2009-09-29 2011-12-09 Ecole Polytech MICROFLUIDIC CIRCUIT
US8415619B2 (en) * 2009-11-13 2013-04-09 University of Glascgow Methods and systems for mass spectrometry
WO2012027366A2 (en) 2010-08-23 2012-03-01 President And Fellows Of Harvard College Acoustic waves in microfluidics
EP2436444A1 (en) 2010-10-01 2012-04-04 Centre National de la Recherche Scientifique (C.N.R.S.) Microfluidic device for production and collection of droplets of a fluid
EP2665957B1 (en) 2011-01-21 2015-03-11 Fluimedix APS Method of controlling a flow
EP2691676B1 (en) 2011-03-30 2019-03-27 Bio-Rad Laboratories, Inc. Injection of multiple volumes into or out of droplets
US9821310B2 (en) 2011-03-31 2017-11-21 The University Of Akron Two-stage microfluidic device for acoustic particle manipulation and methods of separation
US9597692B2 (en) 2011-08-26 2017-03-21 Imec Micro-fluidic device for sorting particles, and methods for sorting particles
US20140227777A1 (en) 2011-09-30 2014-08-14 Brigham And Women's Hospital Cell sorting by 3d flow and adhesive rolling
WO2013116311A1 (en) 2012-01-31 2013-08-08 The Penn State Research Foundation Microfluidic manipulation and sorting of particles using tunable standing surface acoustic wave
WO2014004630A1 (en) 2012-06-27 2014-01-03 President And Fellows Of Harvard College Control of entities such as droplets and cells using acoustic waves
CN104968417B (en) 2012-08-01 2017-08-18 宾夕法尼亚州立大学研究基金会 The high efficiency separation and operation of particle and cell
US20150298157A1 (en) 2012-10-26 2015-10-22 President And Fellows Of Harvard College Systems and methods for droplet production and manipulation using acoustic waves
EP2969222A4 (en) 2013-03-14 2016-11-16 Inguran Llc Apparatus and methods for high throughput sperm sorting
KR101442486B1 (en) 2013-06-07 2014-09-24 아이에스테크놀로지 주식회사 Apparatus and method for separating impurities from fluid using ultrasound
JP6237031B2 (en) 2013-09-18 2017-11-29 凸版印刷株式会社 Component separation method, component analysis method, and component separation apparatus
WO2015200616A1 (en) 2014-06-26 2015-12-30 President And Fellows Of Harvard College Fluid injection using acoustic waves
DK3341116T3 (en) 2015-08-27 2022-05-02 Harvard College SORTING PROCEDURE USING ACOUSTIC WAVES

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100139377A1 (en) * 2008-12-05 2010-06-10 The Penn State Reserch Foundation Particle focusing within a microfluidic device using surface acoustic waves

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
RAVULA, SURENDRA K. ET AL.: "A Microfluidic System Combining Acoustic and Dielectrophoretic Particle Preconcentration and Focusing.", SENSORS AND ACTUATORS B., vol. 130, 2008, pages 645 - 652, XP022550381 *
TSUTSUI, HIDEAKI ET AL.: "Cell Separation by Non-inertial Force Fields in Microfluidic Systems.", MECHANICS RESEARCH COMMUNICATIONS., vol. 36, 2009, pages 92 - 103, XP025914299 *
WOOD, C. D. ET AL.: "Formation and Manipulation of Two-dimensional Arrays of Micron-scale Particles in Microfluidic Systems by Surface Acoustic Waves.", APPLIED PHYSICS LETTERS, vol. 94, 2009, pages 054101 - 054101-3, XP012118923 *

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