WO2008104916A3 - A cell lysis and/or mixing device - Google Patents

A cell lysis and/or mixing device

Info

Publication number
WO2008104916A3
WO2008104916A3 PCT/IB2008/050656 IB2008050656W WO2008104916A3 WO 2008104916 A3 WO2008104916 A3 WO 2008104916A3 IB 2008050656 W IB2008050656 W IB 2008050656W WO 2008104916 A3 WO2008104916 A3 WO 2008104916A3
Authority
WO
Grant status
Application
Patent type
Prior art keywords
chamber
microparticles
cells
volume
mixing
Prior art date
Application number
PCT/IB2008/050656
Other languages
French (fr)
Other versions
WO2008104916A2 (en )
Inventor
Lucas Johannes Anna Maria Beckers
Sergei Shulepov
Maarten A F Vervoort
Original Assignee
Koninkl Philips Electronics Nv
Beckers Lucas Johannes Anna Ma
Sergei Shulepov
Maarten A F Vervoort
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01FMIXING, e.g. DISSOLVING, EMULSIFYING, DISPERSING
    • B01F11/00Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F11/0005Mixing the contents of independent containers, e.g. test-tubes, by shaking or oscillating them
    • B01F11/0022Mixing the contents of independent containers, e.g. test-tubes, by shaking or oscillating them the containers being submitted to a rectilinear movement
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01FMIXING, e.g. DISSOLVING, EMULSIFYING, DISPERSING
    • B01F11/00Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F11/0065Mixers with shaking, oscillating, or vibrating mechanisms the material being contained in a flexible bag submitted to periodical deformation
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01FMIXING, e.g. DISSOLVING, EMULSIFYING, DISPERSING
    • B01F13/00Other mixers; Mixing plant, including combinations of mixers, e.g. of dissimilar mixers
    • B01F13/005Mixers with loose mixing elements, e.g. balls, in a receptacle
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01FMIXING, e.g. DISSOLVING, EMULSIFYING, DISPERSING
    • B01F13/00Other mixers; Mixing plant, including combinations of mixers, e.g. of dissimilar mixers
    • B01F13/005Mixers with loose mixing elements, e.g. balls, in a receptacle
    • B01F13/0052Mixers with loose mixing elements, e.g. balls, in a receptacle using balls as loose mixing element
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01FMIXING, e.g. DISSOLVING, EMULSIFYING, DISPERSING
    • B01F13/00Other mixers; Mixing plant, including combinations of mixers, e.g. of dissimilar mixers
    • B01F13/0059Micromixers
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06Lysis of microorganisms
    • C12N1/066Lysis of microorganisms by physical methods
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06Fluid handling related problems
    • B01L2200/0647Handling flowable solids, e.g. microscopic beads, cells, particles
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0861Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087Multiple sequential chambers
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/12Specific details about materials
    • B01L2300/123Flexible; Elastomeric
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0475Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0481Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers

Abstract

The present invention is related to a cell lysis and/or mixing device, comprising a chamber (11) having a volume, wherein said chamber is filled with microparticles (12), and said chamber is adapted to accommodate cells. The device further comprises means (15) to put said chamber under vibrations. Furthermore, it is provided that the microparticles within the chamber assume between 20 and 75 % of the chamber's volume (Fig. 4). The present invention further provides a process for lysing and/or mixing cells, wherein cells are put in a chamber which is filled with microparticles, the chamber is set under vibrations, wherein further the microparticles assume between 20 and 75 % of the chamber's volume.
PCT/IB2008/050656 2007-02-27 2008-02-22 A cell lysis and/or mixing device WO2008104916A3 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP07103160 2007-02-27
EP07103160.3 2007-02-27

Publications (2)

Publication Number Publication Date
WO2008104916A2 true WO2008104916A2 (en) 2008-09-04
WO2008104916A3 true true WO2008104916A3 (en) 2008-12-11

Family

ID=39721664

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2008/050656 WO2008104916A3 (en) 2007-02-27 2008-02-22 A cell lysis and/or mixing device

Country Status (1)

Country Link
WO (1) WO2008104916A3 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2939445B1 (en) * 2008-12-10 2013-05-03 Biomerieux Sa Automated system of microorganisms present in a sample lysis, extraction and purification of nucleic acids of said microorganisms for analysis.
KR101776215B1 (en) * 2010-10-29 2017-09-08 삼성전자 주식회사 Micro-device for disrupting cells and method of disrupting cells using the same
CN102465110B (en) 2010-10-29 2015-08-19 三星电子株式会社 Means that the cell lysis and cell or virus lysis method
US8895297B2 (en) 2010-10-29 2014-11-25 Samsung Electronics Co., Ltd. Micro-device and methods for disrupting cells
FR2993281B1 (en) * 2012-07-13 2014-07-18 Biomerieux Sa Automated system of microorganisms present in a sample lysis, extraction and purification of nucleic acids of said microorganisms for analysis
EP2705899A1 (en) 2012-09-07 2014-03-12 Fluigent A microfluidic system comprising a homogenizing component
WO2014137499A1 (en) * 2013-03-06 2014-09-12 Blaze Medical Devices, LLC Bead mill and method of use
EP3023150A1 (en) * 2014-11-19 2016-05-25 F. Hoffmann-La Roche AG Particle mixing

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020039783A1 (en) * 1998-12-24 2002-04-04 Cepheid Device and method for lysing cells, spores, or microorganisms
EP1650297A2 (en) * 2004-10-19 2006-04-26 Samsung Electronics Co., Ltd. Method and apparatus for the rapid disruption of cells or viruses using micro magnetic beads and laser
EP1655366A2 (en) * 2004-11-03 2006-05-10 Samsung Electronics Co., Ltd. Apparatus and method for purification of nucleic acids by phase separation using laser and beads
EP1797956A1 (en) * 2005-12-14 2007-06-20 Samsung Electronics Co., Ltd. Microfluidic device and method for concentration and lysis of cells or viruses

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020039783A1 (en) * 1998-12-24 2002-04-04 Cepheid Device and method for lysing cells, spores, or microorganisms
EP1650297A2 (en) * 2004-10-19 2006-04-26 Samsung Electronics Co., Ltd. Method and apparatus for the rapid disruption of cells or viruses using micro magnetic beads and laser
EP1655366A2 (en) * 2004-11-03 2006-05-10 Samsung Electronics Co., Ltd. Apparatus and method for purification of nucleic acids by phase separation using laser and beads
EP1797956A1 (en) * 2005-12-14 2007-06-20 Samsung Electronics Co., Ltd. Microfluidic device and method for concentration and lysis of cells or viruses

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
LEE JEONG-GUN ET AL: "Microchip-based one step DNA extraction and real-time PCR in one chamber for rapid pathogen identification" LAB ON A CHIP, ROYAL SOCIETY OF CHEMISTRY, CAMBRIDGE, GB, vol. 6, no. 7, 1 January 2006 (2006-01-01), pages 886-895, XP002417818 ISSN: 1473-0197 *

Also Published As

Publication number Publication date Type
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