WO2012013960A3 - Tunicamycin gene cluster - Google Patents

Tunicamycin gene cluster Download PDF

Info

Publication number
WO2012013960A3
WO2012013960A3 PCT/GB2011/051395 GB2011051395W WO2012013960A3 WO 2012013960 A3 WO2012013960 A3 WO 2012013960A3 GB 2011051395 W GB2011051395 W GB 2011051395W WO 2012013960 A3 WO2012013960 A3 WO 2012013960A3
Authority
WO
WIPO (PCT)
Prior art keywords
gene cluster
tunicamycin
relates
tunicamycins
heterologo
Prior art date
Application number
PCT/GB2011/051395
Other languages
French (fr)
Other versions
WO2012013960A2 (en
Inventor
Filip Wyszynski
Benjamin Guy Davis
Mervyn Bibb
Andrew Hesketh
Original Assignee
Isis Innovation Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Isis Innovation Limited filed Critical Isis Innovation Limited
Publication of WO2012013960A2 publication Critical patent/WO2012013960A2/en
Publication of WO2012013960A3 publication Critical patent/WO2012013960A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/38—Nucleosides
    • C12P19/385—Pyrimidine nucleosides

Abstract

The invention relates to a gene cluster for the production of tunicamycins and derivatives thereof. The invention also relates to individual polynucleotides from the gene cluster, variants thereof and the polypeptides they encode. The invention further relates to heterologo-us expressions systems and their use to produce a tunicamycin or a derivative thereof.
PCT/GB2011/051395 2010-07-30 2011-07-22 Tunicamycin gene cluster WO2012013960A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1012900.5A GB201012900D0 (en) 2010-07-30 2010-07-30 Tunicamycin gene cluster
GB1012900.5 2010-07-30

Publications (2)

Publication Number Publication Date
WO2012013960A2 WO2012013960A2 (en) 2012-02-02
WO2012013960A3 true WO2012013960A3 (en) 2012-03-29

Family

ID=42799415

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2011/051395 WO2012013960A2 (en) 2010-07-30 2011-07-22 Tunicamycin gene cluster

Country Status (2)

Country Link
GB (1) GB201012900D0 (en)
WO (1) WO2012013960A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201708982D0 (en) 2017-06-06 2017-07-19 Univ Oxford Innovation Ltd Tunicamycin analogues

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4237225A (en) 1978-12-01 1980-12-02 Eli Lilly And Company Process for preparing tunicamycin

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
CHEN WENQING ET AL: "Characterization of the tunicamycin gene cluster unveiling unique steps involved in its biosynthesis.", PROTEIN & CELL, vol. 1, no. 12, December 2010 (2010-12-01), pages 1093 - 1105, XP002661400, ISSN: 1674-8018 *
DATABASE Protein [Online] 8 June 2010 (2010-06-08), "NAD-dependent epimerase/dehydratase", XP002661396, retrieved from NCBI Database accession no. ZP_06773762 *
DATABASE Protein [Online] 8 June 2010 (2010-06-08), "Radical SAM domain protein", XP002661397, retrieved from NCBI Database accession no. ZP_06773761 *
DATABASE UniProt [Online] 14 October 2008 (2008-10-14), "SubName: Full=MoeE5;", XP002661395, retrieved from EBI accession no. UNIPROT:B5GL38 Database accession no. B5GL38 *
LAUER BETTINA ET AL: "A putative enolpyruvyl transferase gene involved in nikkomycin biosynthesis", JOURNAL OF ANTIBIOTICS (TOKYO), vol. 53, no. 4, April 2000 (2000-04-01), pages 385 - 392, XP009153040, ISSN: 0021-8820 *
TSVETANOVA B C ET AL: "Biosynthesis of tunicamycin and metabolic origin of the 11-carbon dialdose sugar, tunicamine", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 277, no. 38, 20 September 2002 (2002-09-20), pages 35289 - 35296, XP002661398, DOI: DOI:10.1074/JBC.M201345200 *
WIDDICK D A ET AL: "Cloning and engineering of the cinnamycin biosynthetic gene cluster from Streptomyces cinnamoneus cinnamoneus DSM 40005.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 100, no. 7, 1 April 2003 (2003-04-01), pages 4316 - 4321, XP002661399, ISSN: 0027-8424 *

Also Published As

Publication number Publication date
GB201012900D0 (en) 2010-09-15
WO2012013960A2 (en) 2012-02-02

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