WO2012002832A1 - The method of selective alkyl-phosphate phases manufacture for liquid chromatography and related techniques - Google Patents

The method of selective alkyl-phosphate phases manufacture for liquid chromatography and related techniques Download PDF

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Publication number
WO2012002832A1
WO2012002832A1 PCT/PL2011/000067 PL2011000067W WO2012002832A1 WO 2012002832 A1 WO2012002832 A1 WO 2012002832A1 PL 2011000067 W PL2011000067 W PL 2011000067W WO 2012002832 A1 WO2012002832 A1 WO 2012002832A1
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WO
WIPO (PCT)
Prior art keywords
alkyl
liquid chromatography
phases
phosphate
phosphorus
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Application number
PCT/PL2011/000067
Other languages
French (fr)
Inventor
Bogusław BUSZEWSKI
Szymon Bocian
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Uniwersytet Mikołaja Kopernika
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Application filed by Uniwersytet Mikołaja Kopernika filed Critical Uniwersytet Mikołaja Kopernika
Publication of WO2012002832A1 publication Critical patent/WO2012002832A1/en

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B01J20/287—Non-polar phases; Reversed phases
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B01J20/3246—Non-macromolecular compounds having a well defined chemical structure
    • B01J20/3257—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one of the heteroatoms nitrogen, oxygen or sulfur together with at least one silicon atom, these atoms not being part of the carrier as such
    • B01J20/3259—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one of the heteroatoms nitrogen, oxygen or sulfur together with at least one silicon atom, these atoms not being part of the carrier as such comprising at least two different types of heteroatoms selected from nitrogen, oxygen or sulfur with at least one silicon atom
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase

Definitions

  • the object of the present invention is the method of selective alkyl-phosphate phases functioning as column packing manufacture for liquid chromatography and related techniques.
  • alkyl-phosphate stationary phases can imitate biological membranes. They are important for predicting properties of newly developed pharmaceuticals, examining their transport through biological membranes as well as interacting with those membranes.
  • the aim of the invention is to develop the synthesis of selective, stable, and reproducible column packing for liquid chromatography applied in reverse phases systems as well as liquid-solid extraction columns used for separating organic compounds of varied polarities.
  • the advantage of the method is high determination selectivity.
  • the method is based on manufacturing alkyl-phosphate stationary phases designed for liquid chromatography with the general formula 1 in which silica gel, previously modified with aminosilane, undergoes a reaction with a alkyl-phosphate ligand in an aprotic organic solvent, favourably in toluene, in the presence of triethylamine as an activating agent.
  • the alkyl-phosphate ligand is obtained in the reaction of alcohol with phosphorus(V) trichloride oxide.
  • the sorbent obtained is purified from the excess of substrates on a filter funnel with a sintered disc of G4 porosity.
  • the sorbent is rinsed with toluene, methanol, and n-hexane, respectively.
  • a hydrophobic stationary phase containing a phosphate group and residual amine groups is received, owing to which chromatographic material gains biological membrane properties.
  • R denotes an alkyl chain.
  • alkyl-phosphate stationary phases By changing the length of an alkyl chain, alkyl-phosphate stationary phases of different hydrophobicity can be obtained. Chromatographic properties of these phases enable analysing compounds of varied polarities, of both acidic and basic character as well as, due to controlled hydrophobicity, non-polar compounds. It results from the structure of the stationary phase containing an alkyl chain and a phosphate group. The phosphate group can dissociate to gain the negative charge which is related to the pH of a mobile phase.
  • the above mentioned stationary phase properties make it applicable also in electro- chromatography or solid phase extraction.
  • the properties of the packing described allow isolating substances such as medicines or narcotics from natural matrices such as blood and urine or pesticides residue from food products.
  • a 5g sample of silica gel modified with y-aminopropyl ligands is dried in vacuum 10 '3 MPa at temperature 120°C for 6 hours in oil bath.
  • the alkyl-phosphate ligand is obtained by stirring a solution of 0.8 ml (9 mmol) phosphorus(V) trichloride oxide (POCI3) in 10 ml anhydrous toluene and 1.7 ml (9 mmol) octanol dissolved in 10 ml anhydrous toluene, for 120 minutes, with a magnetic stirrer.
  • POCI3 phosphorus(V) trichloride oxide
  • the solution containing alkyl-phosphate ligands and 4 ml (30 mmol) triethylamine is added to the reactor with dried, modified silica gel.
  • the reactions are carried out at 100°C for 18 hours.
  • the reaction product is separated from the mixture obtained as a result of the reaction on a sintered glass G4. Then, it is rinsed with toluene, methanol, and hexane, respectively, and finally dried.
  • a 5g sample of silica gel modified with ⁇ -aminopropyl ligands is dried in vacuum 10 "3 MPa at temperature 120°C for 6 hours in oil bath.
  • the alkyl-phosphate ligand is obtained by stirring a solution of 0.8 ml (9 mmol) phosphorus (V) trichloride oxide (POCI3) in 10 ml anhydrous toluene and 2.4 ml (9 mmol) octadecanol dissolved in 10 ml anhydrous toluene, for 120 minutes, with a magnetic stirrer.
  • the reaction product is separated from the mixture obtained as a result of the reaction on a sintered glass G4. Then, it is rinsed with toluene, methanol, and hexane, respectively, and finally dried.
  • alkyl-phosphate phases for selected organic compounds determination e.g. alkyl benzene derivatives, polycyclic aromatic hydrocarbons, or beta-adrenolytic drugs
  • Typical mobile phases used in reverse phases systems in liquid chromatography can be applied as an eluent.

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  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)

Abstract

The method according to the invention is based on obtaining selective packing for liquid chromatography in a reversed system of phases containing alkyl-phosphate ligands according to formula 1, where R denotes an alkyl chain. For this purpose, a multi-stage reaction among an amine phase, phosphorus(V) trichloride oxide, and an alcohol in favourable conditions is performed. The product obtained is separated from the reaction mixture, rinsed and dried. The stationary phases obtained can be successfully applied for separating organic compounds in reverse phases systems in liquid chromatography and for extracting to a stationary phase.

Description

The Method of Selective Alkyl-Phosphate Phases Manufacture for Liquid Chromatography and Related Techniques
The object of the present invention is the method of selective alkyl-phosphate phases functioning as column packing manufacture for liquid chromatography and related techniques.
From the medical and pharmaceutical point of view, alkyl-phosphate stationary phases can imitate biological membranes. They are important for predicting properties of newly developed pharmaceuticals, examining their transport through biological membranes as well as interacting with those membranes.
The aim of the invention is to develop the synthesis of selective, stable, and reproducible column packing for liquid chromatography applied in reverse phases systems as well as liquid-solid extraction columns used for separating organic compounds of varied polarities. The advantage of the method is high determination selectivity.
According to the invention, the method is based on manufacturing alkyl-phosphate stationary phases designed for liquid chromatography with the general formula 1 in which silica gel, previously modified with aminosilane, undergoes a reaction with a alkyl-phosphate ligand in an aprotic organic solvent, favourably in toluene, in the presence of triethylamine as an activating agent. The alkyl-phosphate ligand is obtained in the reaction of alcohol with phosphorus(V) trichloride oxide. The sorbent obtained is purified from the excess of substrates on a filter funnel with a sintered disc of G4 porosity. The sorbent is rinsed with toluene, methanol, and n-hexane, respectively. As a result, a hydrophobic stationary phase containing a phosphate group and residual amine groups is received, owing to which chromatographic material gains biological membrane properties.
In formula 1, R denotes an alkyl chain.
By changing the length of an alkyl chain, alkyl-phosphate stationary phases of different hydrophobicity can be obtained. Chromatographic properties of these phases enable analysing compounds of varied polarities, of both acidic and basic character as well as, due to controlled hydrophobicity, non-polar compounds. It results from the structure of the stationary phase containing an alkyl chain and a phosphate group. The phosphate group can dissociate to gain the negative charge which is related to the pH of a mobile phase. The above mentioned stationary phase properties make it applicable also in electro- chromatography or solid phase extraction.
The properties of the packing described allow isolating substances such as medicines or narcotics from natural matrices such as blood and urine or pesticides residue from food products.
The method based on the invention was exemplified below. The examples, however, do not limit the range of applications protected by the patent.
An elemental analysis, an analysis of infrared and nuclear magnetic resonance spectra confirmed the existence of the functional groups expected on the surface of silica gel
Example 1.
In a reactor that prevents reactants from contacting the surrounding, a 5g sample of silica gel modified with y-aminopropyl ligands is dried in vacuum 10'3 MPa at temperature 120°C for 6 hours in oil bath.
The alkyl-phosphate ligand is obtained by stirring a solution of 0.8 ml (9 mmol) phosphorus(V) trichloride oxide (POCI3) in 10 ml anhydrous toluene and 1.7 ml (9 mmol) octanol dissolved in 10 ml anhydrous toluene, for 120 minutes, with a magnetic stirrer.
Subsequently, the solution containing alkyl-phosphate ligands and 4 ml (30 mmol) triethylamine is added to the reactor with dried, modified silica gel. The reactions are carried out at 100°C for 18 hours. The reaction product is separated from the mixture obtained as a result of the reaction on a sintered glass G4. Then, it is rinsed with toluene, methanol, and hexane, respectively, and finally dried.
Example 2.
In a reactor that prevents reactants from contacting the surrounding, a 5g sample of silica gel modified with γ -aminopropyl ligands is dried in vacuum 10"3 MPa at temperature 120°C for 6 hours in oil bath.
The alkyl-phosphate ligand is obtained by stirring a solution of 0.8 ml (9 mmol) phosphorus (V) trichloride oxide (POCI3) in 10 ml anhydrous toluene and 2.4 ml (9 mmol) octadecanol dissolved in 10 ml anhydrous toluene, for 120 minutes, with a magnetic stirrer.
Subsequently, the solution containing alkyl-phosphate ligands and 4 ml (30 mmol) triethylamine is added to the reactor with dried, modified silica gel. The reactions are carried out at 110°C for 18 hours.
The reaction product is separated from the mixture obtained as a result of the reaction on a sintered glass G4. Then, it is rinsed with toluene, methanol, and hexane, respectively, and finally dried.
According to Example 1 and 2, the application of alkyl-phosphate phases for selected organic compounds determination (e.g. alkyl benzene derivatives, polycyclic aromatic hydrocarbons, or beta-adrenolytic drugs) allows for their complete separation. Typical mobile phases used in reverse phases systems in liquid chromatography can be applied as an eluent.

Claims

We claim:
1. A method of manufacturing selective alkyl-phosphate stationary phases for liquid chromatography of the general formula 1, wherein alkyl dichlorophosphate obtained in a reaction of alcohol dissolved in a non-polar solvent with phosphorus(V) trichloride oxide dissolved in a non-polar organic solvent undergoes a reaction with silica gel previously modified with aminosilane in the presence of an activator.
2. A method according to claim 1, wherein alkyl dichlorophosphate is obtained in the reaction of alcohol with phosphorus (V) trichloride oxide.
3. A method according to claim 1, wherein triethylamine is used as an activator and organic toluene - as a solvent.
4. A method according to claim 1, wherein an alcohol to phosphorus (V) trichloride oxide ratio is in the range between 1:2 and 4:2.
5. A method according to claim 1, wherein the product is purified from an excess of substrates on a filter funnel with a sintered disc of G4 porosity by rinsing with toluene, methanol, and n-hexane, respectively.
PCT/PL2011/000067 2010-07-01 2011-06-30 The method of selective alkyl-phosphate phases manufacture for liquid chromatography and related techniques WO2012002832A1 (en)

Applications Claiming Priority (2)

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PL391699A PL391699A1 (en) 2010-07-01 2010-07-01 Process for the preparation of selective phospho-alkyl phases for liquid chromatography and related techniques
PLP-391699 2010-07-01

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103028383A (en) * 2012-12-31 2013-04-10 浙江月旭材料科技有限公司 Silica gel chromatography packing and preparation method thereof

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2303823A1 (en) * 1975-03-11 1976-10-08 Oxy Metal Industries Corp METHOD AND COMPOSITIONS FOR TREATMENT OF POLYMERIC SUBSTRATES BEFORE THEIR COATING BY NON-ELECTROLYTIC METHOD
US4296047A (en) * 1979-03-24 1981-10-20 Bayer Aktiengesellschaft Continuous preparation of phosphoric acid alkyl ester dichlorides
SU1613130A1 (en) * 1988-02-01 1990-12-15 Киевский Государственный Университет Им.Т.Г.Шевченко Method of producing silica modified with ether of phosphonic acid
WO1991010507A1 (en) * 1990-01-17 1991-07-25 Octapharma Ag Dialkoxyphosphorus compounds bonded to carriers, process for preparing them and their use
DE4207299A1 (en) * 1992-03-07 1993-11-25 Solvay Deutschland New bis:di:alkyl-amino-phosphine oxido-amino-alkyl-tri:alkoxy-silane cpds. supported organo-amino-phosphine oxide catalyst - used in disproportionation of aryl- or alkyl-halo-di:silane cpds. and prepn. of new cpds. and catalyst
WO1994002226A1 (en) * 1992-07-20 1994-02-03 Research Corporation Technologies, Inc. Phosphorylation with monomeric metaphosphates
US5690832A (en) * 1994-09-27 1997-11-25 Syracuse University Chemically active ceramic compositions with a phospho-acid moiety

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2303823A1 (en) * 1975-03-11 1976-10-08 Oxy Metal Industries Corp METHOD AND COMPOSITIONS FOR TREATMENT OF POLYMERIC SUBSTRATES BEFORE THEIR COATING BY NON-ELECTROLYTIC METHOD
US4296047A (en) * 1979-03-24 1981-10-20 Bayer Aktiengesellschaft Continuous preparation of phosphoric acid alkyl ester dichlorides
SU1613130A1 (en) * 1988-02-01 1990-12-15 Киевский Государственный Университет Им.Т.Г.Шевченко Method of producing silica modified with ether of phosphonic acid
WO1991010507A1 (en) * 1990-01-17 1991-07-25 Octapharma Ag Dialkoxyphosphorus compounds bonded to carriers, process for preparing them and their use
DE4207299A1 (en) * 1992-03-07 1993-11-25 Solvay Deutschland New bis:di:alkyl-amino-phosphine oxido-amino-alkyl-tri:alkoxy-silane cpds. supported organo-amino-phosphine oxide catalyst - used in disproportionation of aryl- or alkyl-halo-di:silane cpds. and prepn. of new cpds. and catalyst
WO1994002226A1 (en) * 1992-07-20 1994-02-03 Research Corporation Technologies, Inc. Phosphorylation with monomeric metaphosphates
US5690832A (en) * 1994-09-27 1997-11-25 Syracuse University Chemically active ceramic compositions with a phospho-acid moiety

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* Cited by examiner, † Cited by third party
Title
DATABASE WPI Week 199146, Derwent World Patents Index; AN 1991-337528, XP002662228 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103028383A (en) * 2012-12-31 2013-04-10 浙江月旭材料科技有限公司 Silica gel chromatography packing and preparation method thereof
CN103028383B (en) * 2012-12-31 2014-12-10 浙江月旭材料科技有限公司 Silica gel chromatography packing and preparation method thereof

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