WO2012002798A1 - Agent pour le traitement des eaux usées, procédé d'élaboration de cet agent, et procédé pour le traitement des eaux usées - Google Patents

Agent pour le traitement des eaux usées, procédé d'élaboration de cet agent, et procédé pour le traitement des eaux usées Download PDF

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Publication number
WO2012002798A1
WO2012002798A1 PCT/MY2011/000087 MY2011000087W WO2012002798A1 WO 2012002798 A1 WO2012002798 A1 WO 2012002798A1 MY 2011000087 W MY2011000087 W MY 2011000087W WO 2012002798 A1 WO2012002798 A1 WO 2012002798A1
Authority
WO
WIPO (PCT)
Prior art keywords
wastewater
microalgae
periphytic
treatment
agent
Prior art date
Application number
PCT/MY2011/000087
Other languages
English (en)
Inventor
Mohamed Shariff Mohamed.Din
Fatimah Md. Yusoff
Sanjoy Banerjee
Helena Khatoon
Original Assignee
Universiti Putra Malaysia (Upm)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universiti Putra Malaysia (Upm) filed Critical Universiti Putra Malaysia (Upm)
Publication of WO2012002798A1 publication Critical patent/WO2012002798A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
    • C02F3/327—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae characterised by animals and plants
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/02—Aerobic processes
    • C02F3/10—Packings; Fillings; Grids
    • C02F3/104—Granular carriers
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/105—Phosphorus compounds
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/16—Nitrogen compounds, e.g. ammonia
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/16—Nitrogen compounds, e.g. ammonia
    • C02F2101/166—Nitrites
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/20—Heavy metals or heavy metal compounds
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00—Technologies for wastewater treatment
    • Y02W10/10—Biological treatment of water, waste water, or sewage

Definitions

  • the present invention relates to an agent for the treatment of wastewater containing immobilized periphytic microalgae, to a method of preparation of said agent as well as to a method of treatment of wastewater using immobilized periphytic microalgae for rapid and efficient removal of toxic compounds from wastewater.
  • Microalgae are being used as food, feed and as a source of valuable chemicals.
  • mey are used extensively in the treatment of wastewater because of their ability to sequester large amounts of nutrients such as ammonia, nitrite, phosphate and other pollutants like heavy metals.
  • an ozone generator as an oxidizer has been used to make available organically bound nutrients to attached algae or aquatic plants for treating water.
  • pesticides are added to control insect populations.
  • entrapped and immobilized purified water sludge containing nitrifying bacteria have been used for rapidly activated nitrification activity and stable pellet strength.
  • no invention has been reported using selected immobilized periphytic microalgae for rapid and efficient removal of toxic compounds from wastewater.
  • the method of the present invention is cost effective, environmental friendly and has potential for commercialization.
  • the present invention provides for a rapid and efficient removal of toxic compounds from wastewater by using immobilized periphytic microalgae.
  • the object of the present invention is an agent for the treatment of wastewater containing periphytic microalgae immobilized in alginate beads (oscibeads).
  • the periphytic microalgae are Oscillatoria sp.
  • the present invention also includes a method of preparation of the agent for the treatment of wastewater containing immobilized periphytic microalgae, which comprises the steps of: a) preparing aqueous sodium alginate solution,
  • step b) mixing the solution prepared in step a) with a sodium chloride aqueous solution, c) adding penphytic microaigae cells to the solution prepared in step b),
  • step d) adding the solution prepared in step c) dropwisc to a solution of strontium chloride.
  • the object of the present invention is further a method of treatment of wastewater using the agent of the present invention containing penphytic microaigae immobilized in alginate beads.
  • the penphytic microaigae are Oscillatoria sp.
  • Figure 1 a) Alginate beads contaning penphytic Oscillatoria sp. day 0; b) alginate beads contaning penphytic Oscillatoria sp. day 2; c) control beads without penphytic microaigae.
  • Figure 5 Percent removal of total ammonia nitrogen (TAN), nitrite (NO2-N) and phosphorus (PO 4 -P) using the beads. Error bars represent standard error.
  • the object of the present invention is an agent for the treatment of wastewater containing periphytic microalgae immobilized in alginate beads.
  • the periphytic microalgae are Oscillatoria sp.
  • the growth of microalgae can be controlled-
  • the immobilization of microalgae can control their growth due to their encapsulation within the alginate beads.
  • the immobilization also prevents the release of microalgae into open water bodies when the water is discharged as they can be easily harvested. Algae released into waterbodies along with discharged water can be harmful without proper management due to microalgal bloom causing eutrophication.
  • Microalgal cells entrapped in alginate beads are protected against mechanical stress, while nutrients and metabolites can diffuse through the semi-permeable bead, allowing the maintenance of the cells biological viability.
  • the present invention also includes a method of preparation of the agent for the treatment of wastewater containing immobilized periphytic microalgae, which comprises the steps of:
  • step b) mixing the solution prepared in step a) with a sodium chloride aqueous solution, c) adding periphytic microalgae cells to the solution prepared in step b),
  • step d) adding the solution prepared in step c) drop wise to a solution of strontium chloride.
  • the periphytic microalgae cells used in step c) are preferably purified using spread plate technique followed by mass culturing in larger containers (tanks or photobioreactors) at a temperature of from 20 °C to 45 °C, salinity of from 0 to 40 ppt and at a pH of from 7 to 9, and then centrifuged.
  • the centrifuged microalgae is then added to the solution prepared in step b), preferably to a 273 rd aqueous sodium alginate solution mixed with l/3 rd sodium chloride aqueous solution.
  • the resultant solution containing the microalgae is mixed thoroughly and then dropped, preferably from a height of about 15 cm, to a solution of SrCl2, preferably at a rate of from 30 to 40 drops per minute.
  • the beads are kept in SrC.2 solution for a period of from 45 min to 6 h.
  • the hardened beads obtained in step d) are titien washed with filtered seawater in order to remove any traces of the strontium chloride solution.
  • the prepared beads can men be stored in algal culture medium at about 4 °C for further use.
  • the object of the present invention is further a method of treatment of wastewater using the agent of the present invention containing periphytic microalgae immobilized in alginate beads.
  • the periphytic microalgae are Oscillatoria sp.
  • the treatment of wastewater preferably includes removal of toxic compounds, preferably selected from the group comprising ammonia, nitrite and phosphorous compounds.
  • the method is carried out by contacting the wastewater to be treated with the alginate beads with immobilized periphytic microalgae for a period of one to two days. In case of need, the treatment can be repeated.
  • the agent of the present invention provides for a rapid sequestration of toxic agents from wastewater.
  • the immobilized periphytic Oscillatoria sp. removed in our tests 100% ammonium within 24 h and 69% phosphorus in 48 h.
  • the agent containing alginate beads with immobilized periphytic microalgae is capable of reducing the ammonia content to 0.00 mg L "1 within one day, the nitrite content to 0.00 mg L "1 within one day, and the phosphorou compounds to 2.1 mg L "1 within two days.
  • Periphytic microalgae isolated from the environment were purified and grown in Conway medium agar plates. The plates were incubated at 20-45°C ⁇ 2°C for a week. Colonies were isolated from plates and inoculated into a flask with Conway medium incubated at 20-45°C ⁇ 2°C. Identification of the isolated species was done following standard methods.
  • the beads were kept in the strontium chloride solution for a period of from 45 min to 6 h to allow complete hardening.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Water Supply & Treatment (AREA)
  • Hydrology & Water Resources (AREA)
  • Microbiology (AREA)
  • Environmental & Geological Engineering (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Botany (AREA)
  • Removal Of Specific Substances (AREA)
  • Biological Treatment Of Waste Water (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)

Abstract

La présente invention concerne un agent de traitement des eaux usées, contenant des microalgues périphytiques prises dans des billes d'alginate. L'invention concerne également un procédé d'élaboration dudit agent. L'invention concerne enfin un procédé de traitement des eaux usées utilisant des microalgues périphytiques prises dans des billes d'alginate pour éliminer rapidement et efficacement des composés toxiques contenus dans des eaux usées. Ces billes conviennent effectivement au traitement d'eaux usées contenant des quantités élevées de composés toxiques tels que les composés ammoniaqués, les composés nitrités, et les composés phosphorés solubles réactifs.
PCT/MY2011/000087 2010-06-27 2011-06-08 Agent pour le traitement des eaux usées, procédé d'élaboration de cet agent, et procédé pour le traitement des eaux usées WO2012002798A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
MYPI2010700040 2010-06-27
MYPI2010700040 2010-06-27

Publications (1)

Publication Number Publication Date
WO2012002798A1 true WO2012002798A1 (fr) 2012-01-05

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PCT/MY2011/000087 WO2012002798A1 (fr) 2010-06-27 2011-06-08 Agent pour le traitement des eaux usées, procédé d'élaboration de cet agent, et procédé pour le traitement des eaux usées

Country Status (1)

Country Link
WO (1) WO2012002798A1 (fr)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105668653A (zh) * 2014-11-19 2016-06-15 天津市正水生物科技有限公司 一种水产养殖解毒净水块
CN105668654A (zh) * 2014-11-19 2016-06-15 天津市正水生物科技有限公司 一种水产养殖解毒净水片
EP3098203A1 (fr) 2015-05-26 2016-11-30 Roberto Dal Maschio Procédé d'élimination d'ammoniac à partir d'eaux usées
CN108085313A (zh) * 2017-12-29 2018-05-29 西安石油大学 一种微藻半固化混养处理压裂返排液的方法
CN110214653A (zh) * 2019-07-01 2019-09-10 中国科学院南京土壤研究所 一种利用周丛生物减少稻田磷流失的方法
USRE48523E1 (en) 2012-03-19 2021-04-20 Algae To Omega Holdings, Inc. System and method for producing algae

Citations (2)

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US20050230307A1 (en) 2001-04-19 2005-10-20 Aquafiber Technologies Corporation Periphyton filtration pre- and post-treatment system and method
EP2007032A2 (fr) 2007-05-21 2008-12-24 The Boeing Company Système et procédé de partage global de gestion aérienne de systèmes de données ou autres fichiers de plates-formes aériennes ou autres sources mondiales

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050230307A1 (en) 2001-04-19 2005-10-20 Aquafiber Technologies Corporation Periphyton filtration pre- and post-treatment system and method
EP2007032A2 (fr) 2007-05-21 2008-12-24 The Boeing Company Système et procédé de partage global de gestion aérienne de systèmes de données ou autres fichiers de plates-formes aériennes ou autres sources mondiales

Non-Patent Citations (5)

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Title
DE-BASHAN L E ET AL: "Immobilized microalgae for removing pollutants: Review of practical aspects", BIORESOURCE TECHNOLOGY, ELSEVIER BV, GB, vol. 101, no. 6, 1 March 2010 (2010-03-01), pages 1611 - 1627, XP026813601, ISSN: 0960-8524, [retrieved on 20091221] *
MORENO-GARRIDO ET AL: "Microalgae immobilization: Current techniques and uses", BIORESOURCE TECHNOLOGY, ELSEVIER BV, GB, vol. 99, no. 10, 13 March 2008 (2008-03-13), pages 3949 - 3964, XP022526200, ISSN: 0960-8524, DOI: 10.1016/J.BIORTECH.2007.05.040 *
MUNOZ ET AL: "Algal-bacterial processes for the treatment of hazardous contaminants: A review", WATER RESEARCH, ELSEVIER, AMSTERDAM, NL, vol. 40, no. 15, 1 August 2006 (2006-08-01), pages 2799 - 2815, XP005589997, ISSN: 0043-1354, DOI: 10.1016/J.WATRES.2006.06.011 *
THAKUR, A., KUMAR, H.D.: "Nitrate, ammonium and phosphate uptake by immobilized cells of Dunaliella salina", BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, vol. 62, 1999, pages 70 - 78
THAKUR, KUMAR, EXPERIMENTS, 1999

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USRE48523E1 (en) 2012-03-19 2021-04-20 Algae To Omega Holdings, Inc. System and method for producing algae
CN105668653A (zh) * 2014-11-19 2016-06-15 天津市正水生物科技有限公司 一种水产养殖解毒净水块
CN105668654A (zh) * 2014-11-19 2016-06-15 天津市正水生物科技有限公司 一种水产养殖解毒净水片
CN105668653B (zh) * 2014-11-19 2018-09-04 山东利水环保科技有限公司 一种水产养殖解毒净水块
CN105668654B (zh) * 2014-11-19 2018-09-28 利津县双瀛水产苗种有限责任公司 一种水产养殖解毒净水片
EP3098203A1 (fr) 2015-05-26 2016-11-30 Roberto Dal Maschio Procédé d'élimination d'ammoniac à partir d'eaux usées
CN108085313A (zh) * 2017-12-29 2018-05-29 西安石油大学 一种微藻半固化混养处理压裂返排液的方法
CN110214653A (zh) * 2019-07-01 2019-09-10 中国科学院南京土壤研究所 一种利用周丛生物减少稻田磷流失的方法
CN110214653B (zh) * 2019-07-01 2021-06-25 中国科学院南京土壤研究所 一种利用周丛生物减少稻田磷流失的方法

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