WO2011161545A3 - Non-hydrolyzable protein conjugates, methods and compositions related thereto - Google Patents

Non-hydrolyzable protein conjugates, methods and compositions related thereto Download PDF

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Publication number
WO2011161545A3
WO2011161545A3 PCT/IB2011/002024 IB2011002024W WO2011161545A3 WO 2011161545 A3 WO2011161545 A3 WO 2011161545A3 IB 2011002024 W IB2011002024 W IB 2011002024W WO 2011161545 A3 WO2011161545 A3 WO 2011161545A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
hydrolyzable
protein conjugates
compositions related
hydrolyzable protein
Prior art date
Application number
PCT/IB2011/002024
Other languages
French (fr)
Other versions
WO2011161545A2 (en
Inventor
Huib Ovaa
Anitha Shanmugham
Reggy Ekkebus
Farid El Oualid
Remco Merkx
Original Assignee
The Netherlands Cancer Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The Netherlands Cancer Institute filed Critical The Netherlands Cancer Institute
Publication of WO2011161545A2 publication Critical patent/WO2011161545A2/en
Publication of WO2011161545A3 publication Critical patent/WO2011161545A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6472—Cysteine endopeptidases (3.4.22)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/62—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being a protein, peptide or polyamino acid
    • A61K47/64—Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/62—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being a protein, peptide or polyamino acid
    • A61K47/64—Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent
    • A61K47/646—Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent the entire peptide or protein drug conjugate elicits an immune response, e.g. conjugate vaccines
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/1072—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups
    • C07K1/1075—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups by covalent attachment of amino acids or peptide residues
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/95—Fusion polypeptide containing a motif/fusion for degradation (ubiquitin fusions, PEST sequence)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Public Health (AREA)
  • Wood Science & Technology (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The invention provides compositions and methods relating to non-hydrolyzable post- translational protein modifications, such as, for example, non-hydrolyzable ubiquitin and ubiquitin-like protein conjugates. In some embodiments, the invention relates to antibodies, such as linkage-specific antibodies, directed against a protein conjugate of the invention.
PCT/IB2011/002024 2010-06-04 2011-06-06 Non-hydrolyzable protein conjugates, methods and compositions related thereto WO2011161545A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US35165910P 2010-06-04 2010-06-04
US61/351,659 2010-06-04
US35378010P 2010-06-11 2010-06-11
US61/353,780 2010-06-11

Publications (2)

Publication Number Publication Date
WO2011161545A2 WO2011161545A2 (en) 2011-12-29
WO2011161545A3 true WO2011161545A3 (en) 2012-09-07

Family

ID=45044625

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2011/002024 WO2011161545A2 (en) 2010-06-04 2011-06-06 Non-hydrolyzable protein conjugates, methods and compositions related thereto

Country Status (1)

Country Link
WO (1) WO2011161545A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150158931A1 (en) 2012-07-06 2015-06-11 Stichting Het Nederlands Kanker Instituut Cysteine protease capturing agents
CN106636007B (en) * 2016-12-09 2019-10-29 新乡学院 Anti- USP2a protein monoclonal antibody hybridoma and its anti-USP2a monoclonal antibody and application of generation
NL2022013B1 (en) * 2018-11-16 2020-05-26 Academisch Ziekenhuis Leiden Polypeptide Conjugates

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5545718A (en) * 1989-04-26 1996-08-13 Pasteur Sanofi Diagnostics Synthetic peptides of the conjugate of ubiquitine and H2A histone

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4376110A (en) 1980-08-04 1983-03-08 Hybritech, Incorporated Immunometric assays using monoclonal antibodies
GB8607679D0 (en) 1986-03-27 1986-04-30 Winter G P Recombinant dna product
DE3856559T2 (en) 1987-05-21 2004-04-29 Micromet Ag Multifunctional proteins with predetermined objectives
JPH01207920A (en) 1988-02-16 1989-08-21 Oki Electric Ind Co Ltd Manufacture of inp semiconductor thin film
ATE151110T1 (en) 1988-09-02 1997-04-15 Protein Eng Corp PRODUCTION AND SELECTION OF RECOMBINANT PROTEINS WITH DIFFERENT BINDING SITES
ES2052027T5 (en) 1988-11-11 2005-04-16 Medical Research Council IMMUNOGLOBULINE VARIABLE DOMAIN SEQUENCE CLONING.
US5530101A (en) 1988-12-28 1996-06-25 Protein Design Labs, Inc. Humanized immunoglobulins

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5545718A (en) * 1989-04-26 1996-08-13 Pasteur Sanofi Diagnostics Synthetic peptides of the conjugate of ubiquitine and H2A histone

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ALBERT J. DE GRAAF ET AL: "Nonnatural Amino Acids for Site-Specific Protein Conjugation", BIOCONJUGATE CHEMISTRY, vol. 20, no. 7, 15 July 2009 (2009-07-15), pages 1281 - 1295, XP055025160, ISSN: 1043-1802, DOI: 10.1021/bc800294a *
ANITHA SHANMUGHAM ET AL: "Nonhydrolyzable Ubiquitin-Isopeptide Isosteres as Deubiquitinating Enzyme Probes.", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 132, no. 26, 14 June 2010 (2010-06-14), pages 8834 - 8835, XP055024847, ISSN: 0002-7863, DOI: 10.1021/ja101803s *
NADINE D. WEIKART ET AL: "Generation of Site-Specific and Enzymatically Stable Conjugates of Recombinant Proteins with Ubiquitin-Like Modifiers by the Cu I -Catalyzed Azide-Alkyne Cycloaddition", CHEMBIOCHEM, vol. 11, no. 6, 12 April 2010 (2010-04-12), pages 774 - 777, XP055024843, ISSN: 1439-4227, DOI: 10.1002/cbic.200900738 *
TOMASZ FEKNER ET AL: "Nonenzymatic Ubiquitylation", CHEMBIOCHEM, vol. 12, no. 1, 15 December 2010 (2010-12-15), pages 21 - 33, XP055024976, ISSN: 1439-4227, DOI: 10.1002/cbic.201000625 *

Also Published As

Publication number Publication date
WO2011161545A2 (en) 2011-12-29

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