WO2011154918A3 - Diagnostic apparatus for immunoassay and diagnostic method for immunoassay using the same - Google Patents

Diagnostic apparatus for immunoassay and diagnostic method for immunoassay using the same Download PDF

Info

Publication number
WO2011154918A3
WO2011154918A3 PCT/IB2011/052526 IB2011052526W WO2011154918A3 WO 2011154918 A3 WO2011154918 A3 WO 2011154918A3 IB 2011052526 W IB2011052526 W IB 2011052526W WO 2011154918 A3 WO2011154918 A3 WO 2011154918A3
Authority
WO
WIPO (PCT)
Prior art keywords
immunoassay
fluorescent nanoparticle
unit
microfluidic chip
diagnostic apparatus
Prior art date
Application number
PCT/IB2011/052526
Other languages
French (fr)
Other versions
WO2011154918A2 (en
Inventor
Jaean Jung
Jae Kyung Choi
Young Ho Choi
Original Assignee
Medisensor Gh, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medisensor Gh, Inc. filed Critical Medisensor Gh, Inc.
Publication of WO2011154918A2 publication Critical patent/WO2011154918A2/en
Publication of WO2011154918A3 publication Critical patent/WO2011154918A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • General Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

A diagnostic apparatus for immunoassay is provided. The diagnostic apparatus includes a microfluidic chip having fluorescent nanoparticle-biomaterial composites to detect a biological sample, a light source unit providing flat light to the microfluidic chip to generate fluorescence energy caused by a fluorescence resonance energy transfer (FRET) effect between the fluorescent nanoparticle-biomaterial composites, a sensing unit configured to sense the fluorescence energy emitted from the microfluidic chip, a measuring unit configured to convert the fluorescence energy sensed by the sensing unit into a current peak, a display unit configured to convert the current peak into an image, and a power supply unit using a solar low power circuit to supply power required for operations of the light source unit, the sensing unit, the measuring unit, and the display unit. The fluorescent nanoparticle- biomaterial composite is specifically bound to the biological sample to form a fluorescent nanoparticle-bio-conjugated material composite.
PCT/IB2011/052526 2010-06-09 2011-06-09 Diagnostic apparatus for immunoassay and diagnostic method for immunoassay using the same WO2011154918A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US35301710P 2010-06-09 2010-06-09
US61/353,017 2010-06-09

Publications (2)

Publication Number Publication Date
WO2011154918A2 WO2011154918A2 (en) 2011-12-15
WO2011154918A3 true WO2011154918A3 (en) 2012-04-12

Family

ID=45098470

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2011/052526 WO2011154918A2 (en) 2010-06-09 2011-06-09 Diagnostic apparatus for immunoassay and diagnostic method for immunoassay using the same

Country Status (1)

Country Link
WO (1) WO2011154918A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2529224A4 (en) 2010-01-28 2017-12-13 Ellume Pty Ltd. Sampling and testing device for the human or animal body
US10890590B2 (en) 2012-09-27 2021-01-12 Ellume Limited Diagnostic devices and methods
WO2016115608A1 (en) 2015-01-22 2016-07-28 Ellume Pty Ltd Diagnostic devices and methods for mitigating hook effect and use thereof
CN106855515B (en) * 2017-01-22 2023-11-24 武汉璟泓科技股份有限公司 Colloid Jin Yingguang quantitative analysis integrated machine and control method thereof
EE01561U1 (en) * 2019-02-11 2022-03-15 Qanikdx Oü Method for detection and quantification of at least one organic analyte in a liquid sample.

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5776785A (en) * 1996-12-30 1998-07-07 Diagnostic Products Corporation Method and apparatus for immunoassay using fluorescent induced surface plasma emission
US20030100103A1 (en) * 1995-09-08 2003-05-29 Fujirebio Inc. Immunoassay device and immunoassay method using the same
US20060263907A1 (en) * 2005-04-29 2006-11-23 Zweig Stephen E Fluorescence lateral flow immunoassay
WO2009067521A1 (en) * 2007-11-19 2009-05-28 Florida Atlantic University Microfluidic chips and systems for analyzing protein expression, and methods of use thereof
US7572640B2 (en) * 2004-09-28 2009-08-11 Singulex, Inc. Method for highly sensitive detection of single protein molecules labeled with fluorescent moieties

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030100103A1 (en) * 1995-09-08 2003-05-29 Fujirebio Inc. Immunoassay device and immunoassay method using the same
US5776785A (en) * 1996-12-30 1998-07-07 Diagnostic Products Corporation Method and apparatus for immunoassay using fluorescent induced surface plasma emission
US7572640B2 (en) * 2004-09-28 2009-08-11 Singulex, Inc. Method for highly sensitive detection of single protein molecules labeled with fluorescent moieties
US20060263907A1 (en) * 2005-04-29 2006-11-23 Zweig Stephen E Fluorescence lateral flow immunoassay
WO2009067521A1 (en) * 2007-11-19 2009-05-28 Florida Atlantic University Microfluidic chips and systems for analyzing protein expression, and methods of use thereof

Also Published As

Publication number Publication date
WO2011154918A2 (en) 2011-12-15

Similar Documents

Publication Publication Date Title
WO2011154918A3 (en) Diagnostic apparatus for immunoassay and diagnostic method for immunoassay using the same
EP3501384A3 (en) Method for fluorescence-based imaging and monitoring
WO2013049702A3 (en) Systems and methods for biological analysis
MY158484A (en) Detection apparatus and method for detecting airbone biological particles
WO2013116333A3 (en) Thermal contrast assay and reader
EP2419733A4 (en) Methods and devices for detecting the presence of an analyte in a sample
EP1962085A3 (en) Apparatus for emitting and detecting beams of light
WO2005030030A3 (en) Method and apparatus for ratio fluorometry
EP2569449A4 (en) Identification of nucleic acids
EP2576833A4 (en) Methods and apparatus for point-of-care detection of nucleic acid in a sample
TW200722115A (en) Automated endoscope reprocessor solution testing
GB2484610A (en) Optical approach for microfluidic dna electrophoresis detection
NZ596659A (en) Bulbless spectrometer
WO2008036697A3 (en) Methods, systems and apparatus for light concentrating mechanisms
WO2014197899A3 (en) Methods, systems and apparatus of an all-optics ultrasound sensor
EP2309256A3 (en) Chromatographic measurement apparatus
EP2277047B8 (en) Time-resolved fluorescence energy transfer (TR-FRET) assay for detecting exposure to an infectious organism
WO2013013220A3 (en) Photonic blood typing
WO2010149995A3 (en) Microfluidic device
WO2015067961A3 (en) Skin-print fluorescence analysis method and apparatus
WO2013140350A3 (en) Method and apparatus for characterising samples by measuring light scattering and fluorescence
WO2008029298A3 (en) Optical probe
JP2012137348A5 (en)
WO2012061724A3 (en) Optical sensor and sensing system for oxygen monitoring in fluids using molybdenum cluster phosphorescence
EP2887140A3 (en) Light source unit and projection apparatus

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 11792038

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 11792038

Country of ref document: EP

Kind code of ref document: A2