WO2011142832A3 - Stem cells derived under low oxygen conditions - Google Patents

Stem cells derived under low oxygen conditions Download PDF

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Publication number
WO2011142832A3
WO2011142832A3 PCT/US2011/000850 US2011000850W WO2011142832A3 WO 2011142832 A3 WO2011142832 A3 WO 2011142832A3 US 2011000850 W US2011000850 W US 2011000850W WO 2011142832 A3 WO2011142832 A3 WO 2011142832A3
Authority
WO
WIPO (PCT)
Prior art keywords
oxygen conditions
derived under
stem cells
under low
cells derived
Prior art date
Application number
PCT/US2011/000850
Other languages
French (fr)
Other versions
WO2011142832A2 (en
Inventor
Maisam Mitalipova
Rudolf Jaenisch
Original Assignee
Whitehead Institute For Biomedical Research
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Whitehead Institute For Biomedical Research filed Critical Whitehead Institute For Biomedical Research
Publication of WO2011142832A2 publication Critical patent/WO2011142832A2/en
Publication of WO2011142832A3 publication Critical patent/WO2011142832A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/02—Atmosphere, e.g. low oxygen conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/235—Leukemia inhibitory factor [LIF]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Gynecology & Obstetrics (AREA)
  • Biotechnology (AREA)
  • Reproductive Health (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention provides human ES cell lines derived under physiological oxygen conditions. In some embodiments the invention provides female hES cell lines derived under physiological oxygen conditions, wherein the lines have two active X chromosomes and can undergo X chromosome inactivation. Methods of deriving and using the cell lines are also provided.
PCT/US2011/000850 2010-05-13 2011-05-13 Stem cells derived under low oxygen conditions WO2011142832A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US33447310P 2010-05-13 2010-05-13
US61/334,473 2010-05-13

Publications (2)

Publication Number Publication Date
WO2011142832A2 WO2011142832A2 (en) 2011-11-17
WO2011142832A3 true WO2011142832A3 (en) 2012-03-29

Family

ID=44914887

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2011/000850 WO2011142832A2 (en) 2010-05-13 2011-05-13 Stem cells derived under low oxygen conditions

Country Status (1)

Country Link
WO (1) WO2011142832A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110292038A (en) * 2018-03-23 2019-10-01 北京世纪劲得生物技术有限公司 Frozen solution of mescenchymal stem cell and preparation method thereof

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10119150B2 (en) 2012-05-13 2018-11-06 Allele Biotechnology & Pharmaceuticals, Inc. Feeder-free Derivation of human-induced pluripotent stem cells with synthetic messenger RNA
US10155929B2 (en) * 2012-05-13 2018-12-18 Allele Biotechnology & Pharmaceuticals, Inc. Feeder-free derivation of human-induced pluripotent stem cells with synthetic messenger RNA
GB2504996A (en) * 2012-08-17 2014-02-19 Univ Keele Embryonic stem cell culture method
CA2906197A1 (en) 2013-03-15 2014-09-18 Whitehead Institute For Biomedical Research Cellular discovery platform for neurodegenerative diseases
US10196604B2 (en) 2014-06-20 2019-02-05 The Board Of Trustees Of The Leland Stanford Junior University Mitochondrial inhibitors for use in culturing pluripotent stem cells
EP3191578B1 (en) 2014-09-12 2020-11-04 Whitehead Institute for Biomedical Research Cells expressing apolipoprotein e and uses thereof
CN108841781A (en) * 2018-06-13 2018-11-20 延边大学 Crocin is improving the application in porcine oocytes oxidation resistance and cleavage rates

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
DVASH ET AL.: "Epigenetic regulation of X-inactivation in human embryonic stem cells", EPIGENETICS, vol. 4, no. 1, 1 January 2009 (2009-01-01), pages 19 - 22 *
EZASHI ET AL.: "Low 02 tensions and the prevention of differentiation of hES cells.", PROC NAT ACAD SCI, vol. 102, no. 13, 29 March 2005 (2005-03-29), pages 4783 - 4788, XP002466326, DOI: doi:10.1073/pnas.0501283102 *
FORSYTH ET AL.: "Physiologic oxygen enhances human embryonic stem cell clonal recovery and reduces chromosomal abnormalities.", CLONING STEM CELLS, vol. 8, no. 1, 2006, pages 16 - 23 *
RAJALA ET AL.: "A defined and xeno-free culture method enabling the establishment of clinical- grade human embryonic, induced pluripotent and adipose stem cells. art. e10246", PLOS ONE, vol. 5, no. 4, 19 April 2010 (2010-04-19), pages 1 - 15, XP002626070, DOI: doi:10.1371/journal.pone.0010246 *
VENU ET AL.: "Analysis of long-term culture properties and pluripotent character of two sibling human embryonic stem cell lines derived from discarded embryos.", IN VITRO CELL DEV BIO ANIM, vol. 46, 23 February 2010 (2010-02-23), pages 200 - 205 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110292038A (en) * 2018-03-23 2019-10-01 北京世纪劲得生物技术有限公司 Frozen solution of mescenchymal stem cell and preparation method thereof
CN110292038B (en) * 2018-03-23 2021-08-24 北京世纪劲得生物技术有限公司 Freezing protection solution for mesenchymal stem cells and preparation method thereof

Also Published As

Publication number Publication date
WO2011142832A2 (en) 2011-11-17

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