WO2011141788A1 - Method for extraction of material from a sapindacea family fruit - Google Patents
Method for extraction of material from a sapindacea family fruit Download PDFInfo
- Publication number
- WO2011141788A1 WO2011141788A1 PCT/IB2011/000964 IB2011000964W WO2011141788A1 WO 2011141788 A1 WO2011141788 A1 WO 2011141788A1 IB 2011000964 W IB2011000964 W IB 2011000964W WO 2011141788 A1 WO2011141788 A1 WO 2011141788A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- sapindaceae family
- family fruit
- solvent
- sapindaceae
- fruit
- Prior art date
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
Definitions
- the present invention is related to how to obtain
- Sapindacea family fruit derived material to make a preparation, wherein the preparation is described herein.
- trifoliatus that is used as a treatment for migraine.
- the invention of the present application provides a standardized method to obtain a material from Sapindacea family fruits, wherein the material is utilized by itself or in
- the material by itself has surfactant, emulsifying and foaming properties, among others.
- the invention provides a preparation wherein the material is used in combination with Swinglea glutinosa extract.
- the preparation enhances the ability of the Swinglea glutinosa extract to kill fungi, and to kill and repel insects and mites.
- Sapindaceae family fruit is brownish gold, and wherein said Sapindaceae family fruit skin shows wrinkles;
- the mix of the broken Sapindaceae family fruit skin and pulp, the unbroken Sapindaceae family fruit seed, and the solvent is exposed to activated carbon before separating all solid
- the Sapindaceae family fruit is the Sapindus saponaria fruit.
- the solvent amount is at least one part per one part of the total initial weight of the skin plus the pulp plus the seed.
- the present invention also provides a material derived from a Sapindaceae family fruit, wherein the material is derived from the Sapindaceae family fruit by a method comprising:
- Sapindaceae family fruit is brownish gold, and wherein said Sapindaceae family fruit skin shows wrinkles;
- the mix of broken Sapindaceae family fruit skin and pulp, the unbroken Sapindaceae family fruit seed, and the solvent is exposed to activated carbon before separating all solid
- the Sapindaceae family fruit is the Sapindus
- the solvent amount is at least one part per one part of the total initial weight of the skin plus the pulp plus the seed .
- the present invention provides a preparation that comprises an extract from Swinglea glutinosa and a material derived from a Sapindaceae family fruit. 0016.
- the material is derived from the Sapindaceae family- fruit by a method comprising:
- Sapindaceae family fruit is brownish gold, and wherein said Sapindaceae family fruit skin shows wrinkles;
- the mix of broken Sapindaceae family fruit skin and pulp, the unbroken Sapindaceae family fruit seed, and the solvent is exposed to activated carbon before separating all solid particles.
- the Sapindaceae family fruit is the Sapindus
- the solvent amount is at least one part per one part of the total initial weight of the skin plus the pulp plus the seed.
- the preparation of the present invention can be used to kill fungi, to kill insects and mites, and also to repel insects and mites .
- the present Application invention also provides an extract isolated from Swinglea glutinosa leaves, wherein said extract is obtained by a method comprising:
- step D repeat step D. until the solvent is enriched with
- the present invention provides a method to extract a material from the Sapindaceae family fruit, wherein the method comprises:
- Sapindaceae family fruit is brownish gold, and wherein said Sapindaceae family fruit skin shows wrinkles;
- step of "retiring the solvent from the liquid portion” said step can be performed by evaporating the solvent from the liquid portion.
- “retiring the solvent from the liquid portion” can also be achieved by lyophilization, dry spraying, etc.
- the mix of the broken Sapindaceae family fruit skin and pulp, the unbroken Sapindaceae family fruit seed, and the solvent is exposed to activated carbon before separating all solid particles.
- all solid particles can be separated without exposing the mix of the broken Sapindaceae family fruit skin and pulp, the unbroken Sapindaceae family fruit seed, and the solvent to activated carbon.
- activated carbon refers to any form of porous carbon with enough surface area for adsorption or chemical reactions.
- Activated carbon is synonymous to "activated charcoal” or “activated coal” .
- the Sapindaceae family fruit is the Sapindus saponaria fruit.
- the solvent amount is at least one part per one part of the total initial weight of the skin plus the pulp plus the seed .
- the present invention also provides a material derived from a Sapindaceae family fruit, wherein the material is derived from the Sapindaceae family fruit by a method comprising:
- Sapindaceae family fruit is brownish gold, and wherein said Sapindaceae family fruit skin shows wrinkles;
- the mix of broken Sapindaceae family fruit skin and pulp, the unbroken Sapindaceae family fruit seed, and the solvent is exposed to activated carbon before separating all solid
- the Sapindaceae family fruit is the Sapindus
- the solvent amount is at least one part per one part of the total initial weight of the skin plus the pulp plus the seed. 0033. Furthermore, the present invention provides a preparation that comprises an extract from Swinglea glutinosa and a material derived from a Sapindaceae family fruit.
- the material is derived from the Sapindaceae family fruit by a method comprising:
- Sapindaceae family fruit is brownish gold, and wherein said Sapindaceae family fruit skin shows wrinkles;
- the mix of broken Sapindaceae family fruit skin and pulp, the unbroken Sapindaceae family fruit seed, and the solvent is exposed to activated carbon before separating all solid particles.
- the material, the method and the preparation the amount of carbon is one part to one to three parts the amount of the mix.
- the mix of broken the mix of broken Sapindaceae family fruit skin and pulp, the unbroken Sapindaceae family fruit seed, and the solvent could be evaporated to retire the solvent without exposition to activated carbon.
- the resulting material is translucent crystal.
- the material obtained is turbid and dark.
- the material maintains the surfactant, emulsifying and foaming properties.
- the material can be used to be combined with the Swinglea glutinosa extract to make the preparation.
- the Sapindaceae family fruit is the Sapindus
- the solvent amount is at least one part per one part of the total initial weight of the skin plus the pulp plus the seed.
- the preparation of the present invention can be used to kill fungi, wherein susceptible fungi comprises:
- to kill fungi comprises “to prevent fungi” .
- the preparation of the present invention can be used to kill insects and mites, and also to repel insects and mites, wherein susceptible insects and mites comprise:
- the present Application invention also provides an extract isolated from Swinglea glutinosa leaves, wherein said extract is obtained by a method comprising:
- step D repeat step D. until the solvent is enriched with
- the leaves in the method, must not be broken into leaf fragments that are less than 0.5 mm, since smaller fragments would tend to become a single mass which will become a limitation for the optimal extraction with a solvent.
- the solvent that can be used comprises ethanol, methanol, hexane, propanol , isopropanol, C0 2 , acetone, water, ethyl -acetate, nitrile-acetate , toluene, tetrahydrofurane , Chloroform, dichloromethane , and others.
- Sapindaceae family fruit and the Swinglea glutinosa extract of the present invention can be combined with camphor and oils that have been described to kill fungi, kill and or repel insects and mites, wherein the oils would enhance the effects of the preparation against fungi, insects , and mites, and wherein the oils can be derived from garlic, orange, lemon, lime,
- Cymbopogon sp. Eugenia caryophyllata, Eucalyptus sp . , Melaleuca altemifolia, Citrus simensis, other citrus sp., cinnamon, and others .
- suspension on water was prepared at a concentration of 1x10 s spores/ml.
- Four plates were prepared: one control plate with the culture medium only and three other plates, wherein the three other plates have the cultured medium and 2ml of each solution. All plates were bathed with 20 ⁇ of the suspension with spores. 0051. After incubation of the plates for 12 days at room temperature the percentage of inhibition for each fungus was measured with the following results:
- Liriomyza sp . in plants with bathed and un-bathed leaves were observed.
- the percentage of inhibition (repellence) in plants with bathed leaves in relation to plants with un-bathed leaves can be observed in the following results:
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Agronomy & Crop Science (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cosmetics (AREA)
- Fats And Perfumes (AREA)
Abstract
The invention of the present application provides a standardized method to obtain a material from Sapindacea family fruits, wherein the material is utilized by itself or in combination with other compounds to make preparations. The material by itself has surfactant, emulsifying and foaming properties, among others. In addition, the invention provides a preparation wherein the material is used in combination with Swinglea glutinosa extract. The preparation enhances the ability of the Swinglea glutinosa extract to kill and prevent fungi, and to kill and repel insects and mites.
Description
METHOD FOR EXTRACTION OF MATERIAL FROM A Sapindacea FAMILY FRUIT
BACKGROUND OF THE INVENTION
1. AREA OF THE INVENTION
0001. The present invention is related to how to obtain
material from a Sapindacea family fruit, and uses of the
material alone or combined in preparations with other compounds. In previous pending unpublished U.S. Patent Application
12/466,801, which is incorporated herein in its entirety by reference, the same inventors of the present application
described an extract that is used in combination with the
Sapindacea family fruit derived material to make a preparation, wherein the preparation is described herein.
2. DESCRIPTION OF PRIOR ART
0002. Sapindacea family fruit derived materials had been described to have beneficial uses.
0003. For example, Kumar Arora S. et al . in U.S. Publication No. 2005/0249831 Al discloses an extract from Sapindus
trifoliatus that is used as a treatment for migraine.
0004. Kusano H. et al . in U.S. Publication No. 2007/0140984 Al also mentions that components derived from Sapindus mukurossi plants have antibacterial effects.
0005. Unfortunately, there is no description in the prior art of a standardized method to obtain a good yield of a Sapindacea
family fruit derived material that can be utilized by itself or in combination to make preparations from which further
beneficial uses can be attained. The Invention of the present Application overcomes these prior art limitations.
SUMMARY OF THE INVENTION
0006. The invention of the present application provides a standardized method to obtain a material from Sapindacea family fruits, wherein the material is utilized by itself or in
combination with other compounds to make preparations. The material by itself has surfactant, emulsifying and foaming properties, among others. In addition, the invention provides a preparation wherein the material is used in combination with Swinglea glutinosa extract. The preparation enhances the ability of the Swinglea glutinosa extract to kill fungi, and to kill and repel insects and mites.
0007. More specifically, the invention of the present
application provides a method to extract a material from the Sapindaceae family fruit, wherein the method comprises:
A. Obtaining a Sapindaceae family fruit, wherein the
Sapindaceae family fruit is brownish gold, and wherein said Sapindaceae family fruit skin shows wrinkles;
B. Breaking the Sapindaceae family fruit skin and pulp
without breaking the Sapindaceae family fruit seed;
C. Exposing the broken Sapindaceae family fruit skin and pulp and the unbroken Sapindaceae family fruit seed to a solvent ;
D. Separating all solid particles from the mix of broken Sapindaceae family fruit skin and pulp, the unbroken Sapindaceae family fruit seed, and the solvent, wherein a liquid portion remains;
E. Retiring the solvent from the liquid portion, wherein the liquid portion without the solvent constitutes the material .
0008. In one aspect of the method of the present invention, the mix of the broken Sapindaceae family fruit skin and pulp, the unbroken Sapindaceae family fruit seed, and the solvent is exposed to activated carbon before separating all solid
particles .
0009. In another aspect of the method of the present invention, the Sapindaceae family fruit is the Sapindus saponaria fruit.
0010. In one more aspect of the method of the present
invention, the solvent amount is at least one part per one part of the total initial weight of the skin plus the pulp plus the seed.
0011. The present invention also provides a material derived from a Sapindaceae family fruit, wherein the material is derived from the Sapindaceae family fruit by a method comprising:
A. Obtaining a Sapindaceae family fruit, wherein the
Sapindaceae family fruit is brownish gold, and wherein said Sapindaceae family fruit skin shows wrinkles;
B. Breaking the Sapindaceae family fruit skin and pulp
without breaking the Sapindaceae family fruit seed;
C. Exposing the broken Sapindaceae family fruit skin and pulp and the unbroken Sapindaceae family fruit seed to a solvent ;
D. Separating all solid particles from the mix of broken
Sapindaceae family fruit skin and pulp, the unbroken Sapindaceae family fruit seed, and the solvent, wherein a liquid portion remains;
E. Retiring the solvent from the liquid portion, wherein the liquid portion without the solvent constitutes the material .
0012. In one aspect of the material of the present invention, the mix of broken Sapindaceae family fruit skin and pulp, the unbroken Sapindaceae family fruit seed, and the solvent is exposed to activated carbon before separating all solid
particles .
0013. In another aspect of the material of the present
invention, the Sapindaceae family fruit is the Sapindus
saponaria fruit.
0014. In one more aspect of the material of the present
invention, the solvent amount is at least one part per one part of the total initial weight of the skin plus the pulp plus the seed .
0015. Furthermore, the present invention provides a preparation that comprises an extract from Swinglea glutinosa and a material derived from a Sapindaceae family fruit.
0016. In one main aspect of the preparation of the present invention, the material is derived from the Sapindaceae family- fruit by a method comprising:
A. Obtaining a Sapindaceae family fruit, wherein the
Sapindaceae family fruit is brownish gold, and wherein said Sapindaceae family fruit skin shows wrinkles;
B. Breaking the Sapindaceae family fruit skin and pulp
without breaking the Sapindaceae family fruit seed;
C. Exposing the broken Sapindaceae family fruit skin and
pulp and the unbroken Sapindaceae family fruit seed to a solvent ;
D. Separating all solid particles from the mix of broken
Sapindaceae family fruit skin and pulp, the unbroken Sapindaceae family fruit seed, and the solvent, wherein a liquid portion remains;
E. Retiring the solvent from the liquid portion, wherein the liquid portion without the solvent constitutes the material .
0017. In one more aspect of the preparation of the present invention, the mix of broken Sapindaceae family fruit skin and pulp, the unbroken Sapindaceae family fruit seed, and the solvent is exposed to activated carbon before separating all solid particles.
0018. In another aspect of the preparation of the present invention, the Sapindaceae family fruit is the Sapindus
saponaria fruit .
0019. In one additional aspect of the preparation of the present invention, the solvent amount is at least one part per one part of the total initial weight of the skin plus the pulp plus the seed.
0020. The preparation of the present invention can be used to kill fungi, to kill insects and mites, and also to repel insects and mites .
0021. In addition, the present Application invention also provides an extract isolated from Swinglea glutinosa leaves, wherein said extract is obtained by a method comprising:
A. Exposing bright dark green Swinglea glutinosa leaves in a dry environment, wherein said leaves are protected from direct sunlight, wherein the leaves are exposed to air flow;
B. leaving the leaves in the environment described in A. until the leaves turn opaque green and the leaves are brittle ;
C. breaking up the leaves into small fragments;
D. putting the Swinglea glutinosa leaf fragments in contact with a solvent at least once;
E. repeat step D. until the solvent is enriched with
substances derived from the leaf fragments; and,
F. retiring the solvent to release the extract.
0022. Objectives and advantages of the present Application invention will be more evident in the detailed description of the invention and the claims .
DETAILED DESCRIPTION OF THE INVENTION
0023. The present invention provides a method to extract a material from the Sapindaceae family fruit, wherein the method comprises:
A. Obtaining a Sapindaceae family fruit, wherein the
Sapindaceae family fruit is brownish gold, and wherein said Sapindaceae family fruit skin shows wrinkles;
B. Breaking the Sapindaceae family fruit skin and pulp
without breaking the Sapindaceae family fruit seed;
C. Exposing the broken Sapindaceae family fruit skin and
pulp and the unbroken Sapindaceae family fruit seed to a solvent ;
D. Separating all solid particles from the mix of broken
Sapindaceae family fruit skin and pulp, the unbroken Sapindaceae family fruit seed, and the solvent, wherein a liquid portion remains;
E. Retiring the solvent from the liquid portion, wherein the liquid portion without the solvent constitutes the material .
0024. Preferably, in all cases in the present application, where the step of "retiring the solvent from the liquid portion" is mentioned, said step can be performed by evaporating the solvent from the liquid portion. However, "retiring the solvent from the liquid portion" can also be achieved by lyophilization, dry spraying, etc.
0025. In one optional aspect of the method of the present invention, the mix of the broken Sapindaceae family fruit skin
and pulp, the unbroken Sapindaceae family fruit seed, and the solvent is exposed to activated carbon before separating all solid particles. However, all solid particles can be separated without exposing the mix of the broken Sapindaceae family fruit skin and pulp, the unbroken Sapindaceae family fruit seed, and the solvent to activated carbon.
0026. For the purpose of this application, the term activated carbon refers to any form of porous carbon with enough surface area for adsorption or chemical reactions. Activated carbon is synonymous to "activated charcoal" or "activated coal" .
0027. In another aspect of the method of the present invention, the Sapindaceae family fruit is the Sapindus saponaria fruit.
0028. In one more aspect of the method of the present
invention, the solvent amount is at least one part per one part of the total initial weight of the skin plus the pulp plus the seed .
0029. The present invention also provides a material derived from a Sapindaceae family fruit, wherein the material is derived from the Sapindaceae family fruit by a method comprising:
A. Obtaining a Sapindaceae family fruit, wherein the
Sapindaceae family fruit is brownish gold, and wherein said Sapindaceae family fruit skin shows wrinkles;
B. Breaking the Sapindaceae family fruit skin and pulp
without breaking the Sapindaceae family fruit seed;
Exposing the broken Sapindaceae family fruit skin and pulp and the unbroken Sapindaceae family fruit seed to a solvent ;
Separating all solid particles from the mix of broken Sapindaceae family fruit skin and pulp, the unbroken Sapindaceae family fruit seed, and the solvent, wherein a liquid portion remains;
Retiring the solvent from the liquid portion, wherein the liquid portion without the solvent constitutes the material .
0030. In one aspect of the material of the present invention, the mix of broken Sapindaceae family fruit skin and pulp, the unbroken Sapindaceae family fruit seed, and the solvent is exposed to activated carbon before separating all solid
particles. However, all solid particles can be separated without exposing the mix of the broken Sapindaceae family fruit skin and pulp, the unbroken Sapindaceae family fruit seed, and the solvent to activated carbon.
0031. In another aspect of the material of the present
invention, the Sapindaceae family fruit is the Sapindus
saponaria fruit .
0032. In one more aspect of the material of the present
invention, the solvent amount is at least one part per one part of the total initial weight of the skin plus the pulp plus the seed.
0033. Furthermore, the present invention provides a preparation that comprises an extract from Swinglea glutinosa and a material derived from a Sapindaceae family fruit.
0034. In one main aspect of the preparation of the present invention, the material is derived from the Sapindaceae family fruit by a method comprising:
A. Obtaining a Sapindaceae family fruit, wherein the
Sapindaceae family fruit is brownish gold, and wherein said Sapindaceae family fruit skin shows wrinkles;
B. Breaking the Sapindaceae family fruit skin and pulp
without breaking the Sapindaceae family fruit seed;
C. Exposing the broken Sapindaceae family fruit skin and
pulp and the unbroken Sapindaceae family fruit seed to a solvent ;
D. Separating all solid particles from the mix of broken
Sapindaceae family fruit skin and pulp, the unbroken Sapindaceae family fruit seed, and the solvent, wherein a liquid portion remains;
E. Retiring the solvent from the liquid portion, wherein the liquid portion without the solvent constitutes the material .
0035. In one more optional aspect of the preparation of the present invention, the mix of broken Sapindaceae family fruit skin and pulp, the unbroken Sapindaceae family fruit seed, and the solvent is exposed to activated carbon before separating all solid particles.
0036. For all cases in this application, the material, the method and the preparation, the amount of carbon is one part to one to three parts the amount of the mix. However, the mix of broken the mix of broken Sapindaceae family fruit skin and pulp, the unbroken Sapindaceae family fruit seed, and the solvent, could be evaporated to retire the solvent without exposition to activated carbon.
0037. When the mix is exposed to activated carbon, the
resulting material is translucent crystal. When the mix is not exposed to activated carbon the material obtained is turbid and dark. In both cases, the material maintains the surfactant, emulsifying and foaming properties. In either of both cases, the material can be used to be combined with the Swinglea glutinosa extract to make the preparation.
0038. In another aspect of the preparation of the present invention, the Sapindaceae family fruit is the Sapindus
saponaria fruit.
0039. In one additional aspect of the preparation of the present invention, the solvent amount is at least one part per one part of the total initial weight of the skin plus the pulp plus the seed.
0040. The preparation of the present invention can be used to kill fungi, wherein susceptible fungi comprises:
Sphaerotheca pannosa
Botritys sp .
Fusarium sp.
Colletotrichum sp.
Uncinola necator
Variola sp.
Peronospora sp.
Puccinia sp.
Cladosporium sp. / Hetesroporium sp.,
and others .
0041. For the purpose of the present application the term "to kill fungi" comprises "to prevent fungi" .
0042. Moreover, the preparation of the present invention can be used to kill insects and mites, and also to repel insects and mites, wherein susceptible insects and mites comprise:
Liriomyza sp
Tetranichus sp .
Boophilus microplus,
and others .
0043. In addition, the present Application invention also provides an extract isolated from Swinglea glutinosa leaves, wherein said extract is obtained by a method comprising:
A. Exposing bright dark green Swinglea glutinosa leaves in a dry environment, wherein said leaves are protected from direct sunlight, wherein the leaves are exposed to air flow;
B. leaving the leaves in the environment described in A. until the leaves turn opaque green and the leaves are brittle ;
C. breaking up the leaves into small fragments;
D. putting the Swinglea glutinosa leaf fragments in contact with a solvent at least once;
E. repeat step D. until the solvent is enriched with
substances derived from the leaf fragments; and,
F. retiring the solvent to release the extract.
0044 . In one more aspect of the Swinglea glutinosa extract of the present invention, in the method, the leaves must not be broken into leaf fragments that are less than 0.5 mm, since smaller fragments would tend to become a single mass which will become a limitation for the optimal extraction with a solvent.
0045 . In all cases where a solvent is mentioned in this
application, the solvent that can be used comprises ethanol, methanol, hexane, propanol , isopropanol, C02, acetone, water, ethyl -acetate, nitrile-acetate , toluene, tetrahydrofurane , Chloroform, dichloromethane , and others.
0046 . In all cases in this application, where solid particles are separated from the mix of broken Sapindaceae family fruit skin and pulp, the unbroken Sapindaceae family fruit seed, and a solvent, the preferred method of separation is centrifugation, although other methods of separation can be used.
0047 . The preparation, with the material derived from a
Sapindaceae family fruit and the Swinglea glutinosa extract of the present invention, can be combined with camphor and oils that have been described to kill fungi, kill and or repel insects and mites, wherein the oils would enhance the effects of the preparation against fungi, insects , and mites, and wherein
the oils can be derived from garlic, orange, lemon, lime,
Cymbopogon sp. , Eugenia caryophyllata, Eucalyptus sp . , Melaleuca altemifolia, Citrus simensis, other citrus sp., cinnamon, and others .
0048 . Objectives and advantages of the present Application invention will be more evident in the detailed description of the invention and the claims.
EXAMPLES
0049 . Three solutions were prepared: 1) A solution of a 2ml material/Liter of water (material derived from a Sapindaceae family fruit wherein the material was obtained as described above in this application) ; 2) A solution of 2ml extract/Liter of water (extract derived from Swinglea glutinosa leaves wherein the extract was obtained as described above in this application) 3) A solution of a 2ml preparation/Liter of water (preparation made by combining equal parts of the material and the extract) . Solutions 1, 2 and 3 were used for the following experiment:
0050 . An isolated strain of a pathogenic fungus was propagated in an adequate culture medium for the strain. Then, a
suspension on water was prepared at a concentration of 1x10s spores/ml. Four plates were prepared: one control plate with the culture medium only and three other plates, wherein the three other plates have the cultured medium and 2ml of each solution. All plates were bathed with 20 μΐ of the suspension with spores.
0051. After incubation of the plates for 12 days at room temperature the percentage of inhibition for each fungus was measured with the following results:
Number of repeats (inhibition %)
Average Standard
Code
(inhibition %) Deviation
1 2 3
SS (material) 75,66 75,47 78,82 76,7 1 ,9
SW (extract) 87,5 89,9 93,2 90,2 2,9
SW+ SS 75,1 84,3 81 ,7 80,4 4,7
(preparation)
0052. With respect to Tetranichus sp. a repellence test was made by comparing 5mm discs cut from leaves of bean plants, wherein 5mm leaf discs that were bathed with 2ml of each one of the three solutions (solutions 1, 2 and 3) were compared against un-bathed 5mm leaf discs, and wherein a 2mm un-bathed leave disc with a Tetranichus sp. mites was put on top of all 5mm leaf discs. The percentage of inhibition (repellence) can be
observed in the following results:
Extract/Liter of each one of the three solutions (solutions 1, 2 and 3) and un-bathed leaves of bean plants. The plants with bathed and un-bathed leaves were put inside a cage with
Liriomyza sp .. After 24 hours, points of disease caused by
Liriomyza sp . in plants with bathed and un-bathed leaves were observed. The percentage of inhibition (repellence) in plants with bathed leaves in relation to plants with un-bathed leaves can be observed in the following results:
0054 . For Sphaerotheca pannosa a spore suspension was applied to leaves of healthy rose plants bathed with 2ml of each one of the three solutions (solutions 1, 2 and 3) and to leaves of un- bathed healthy rose plants. Then the leaves of bathed rose plants and un-bathed rose plants were observed for formation of fungi pustules after 15 days. There was between 81.1% and 87.8% less formation of pustules (inhibition) in the leaves of rose plants bathed with the 2ml the preparation/Liter of water solution, as the following results show:
Number of Repeats (inhibition %) Average
Standard
Code (inhibition
1 2 3 Deviation
%)
SS (Material) 53,1 58,5 51 ,6 54,4 3,6
SW (Extract) 62,4 71 ,3 63,4 65,7 4,9
SW+ SS 84,6 81 ,1 87,8 84,5 3,4 (Preparation)
Claims
1. A method to extract a material from the Sapindaceae
family fruit, wherein the method comprises:
A. Obtaining a Sapindaceae family fruit, wherein the
Sapindaceae family fruit is brownish gold, and wherein said Sapindaceae family fruit skin shows wrinkles;
B. Breaking the Sapindaceae family fruit skin and pulp
without breaking the Sapindaceae family fruit seed;
C. Exposing the broken Sapindaceae family fruit skin and
pulp and the unbroken Sapindaceae family fruit seed to a solvent ;
D. Separating all solid particles from the mix of broken
Sapindaceae family fruit skin and pulp, the unbroken Sapindaceae family fruit seed, and the solvent, wherein a liquid portion remains;
E. Retiring the solvent from the liquid portion, wherein the liquid portion without the solvent constitutes the material .
2. The method of Claim 1, wherein the mix of broken
Sapindaceae family fruit skin and pulp, the unbroken
Sapindaceae family fruit seed, and the solvent is exposed to activated carbon before separating all solid particles.
3. The method of Claim 1, wherein the Sapindaceae family
fruit is the Sapindus saponaria fruit.
The method of Claim 1, wherein the solvent amount is at least one part per one part of the total initial weight of the skin plus the pulp plus the seed.
A material derived from a Sapindaceae family fruit, wherein the material is derived from the Sapindaceae family fruit by a method comprising:
A. Obtaining a Sapindaceae family fruit, wherein the
Sapindaceae family fruit is brownish gold, and wherein said Sapindaceae family fruit skin shows wrinkles;
B. Breaking the Sapindaceae family fruit skin and pulp
without breaking the Sapindaceae family fruit seed;
C. Exposing the broken Sapindaceae family fruit skin and
pulp and the unbroken Sapindaceae family fruit seed to a solvent ;
D. Separating all solid particles from the mix of broken
Sapindaceae family fruit skin' and pulp, the unbroken Sapindaceae family fruit seed, and the solvent, wherein a liquid portion remains;
E. Retiring the solvent from the liquid portion, wherein the liquid portion without the solvent constitutes the material .
The material of Claim 5, wherein the mix of broken
Sapindaceae family fruit skin and pulp, the unbroken
Sapindaceae family fruit seed, and the solvent is exposed to activated carbon before separating all solid particles.
7. The material of Claim 5, wherein the Sapindaceae family- fruit is the Sapindus saponaria fruit.
8. The material of Claim 5, wherein the solvent amount is at least one part per one part of the total initial weight of the skin plus the pulp plus the seed.
9. A preparation that comprises an extract from Swinglea glutinosa and a material derived from a Sapindaceae family fruit .
10. The preparation of Claim 9, wherein the material is
derived from the Sapindaceae family fruit by a method comprising :
A. Obtaining a Sapindaceae family fruit, wherein the
Sapindaceae family fruit is brownish gold, and wherein said Sapindaceae family fruit skin shows wrinkles;
B. Breaking the Sapindaceae family fruit skin and pulp
without breaking the Sapindaceae family fruit seed;
C. Exposing the broken Sapindaceae family fruit skin and
pulp and the unbroken Sapindaceae family fruit seed to a solvent ;
D. Separating all solid particles from the mix of broken
Sapindaceae family fruit skin and pulp, the unbroken Sapindaceae family fruit seed, and the solvent, wherein a liquid portion remains;
E. Retiring the solvent from the liquid portion, wherein the liquid portion without the solvent constitutes the material .
11. The preparation of Claim 10, wherein the mix of broken Sapindaceae family fruit skin and pulp, the unbroken
Sapindaceae family fruit seed, and the solvent is exposed to activated carbon before separating all solid particles.
12. The preparation of Claim 10, wherein the Sapindaceae
family fruit is the Sapindus saponaria fruit.
13. The preparation of Claim 10, wherein the solvent amount is at least one part per one part of the total initial weight of the skin plus the · pulp plus the seed.
14. The preparation of Claim 9, wherein the preparation is used to kill fungi.
15. The preparation of Claim 9, wherein the preparation is used to kill insects and mites.
16. The preparation of Claim 9, wherein the preparation is used to repel insects and mites.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2011251744A AU2011251744A1 (en) | 2010-05-10 | 2011-05-05 | Method for extraction of material from a Sapindacea family fruit |
| BR112012028716A BR112012028716A2 (en) | 2010-05-10 | 2011-05-05 | method for extracting material from a fruit of the sapindacea family |
| EP11780277.7A EP2568813A4 (en) | 2010-05-10 | 2011-05-05 | Method for extraction of material from a sapindacea family fruit |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US12/776,511 US8298590B2 (en) | 2010-05-10 | 2010-05-10 | Method for extraction of material from a Sapindacea family fruit |
| US12/776,511 | 2010-05-10 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO2011141788A1 true WO2011141788A1 (en) | 2011-11-17 |
Family
ID=44902105
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/IB2011/000964 WO2011141788A1 (en) | 2010-05-10 | 2011-05-05 | Method for extraction of material from a sapindacea family fruit |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US8298590B2 (en) |
| EP (1) | EP2568813A4 (en) |
| AR (1) | AR083149A1 (en) |
| AU (1) | AU2011251744A1 (en) |
| BR (1) | BR112012028716A2 (en) |
| CO (1) | CO6602114A2 (en) |
| UY (1) | UY33374A (en) |
| WO (1) | WO2011141788A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103444805A (en) * | 2013-09-10 | 2013-12-18 | 苏州市丰澄农业专业合作社 | Ilex pubescens plant based nano slow-release suspending agent |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8993011B2 (en) * | 2009-05-15 | 2015-03-31 | Ecoflora Agro Sas | Swinglea glutinosa leaves derived material in combination with pyrethroids for control of acari |
| CN104824039A (en) * | 2015-06-02 | 2015-08-12 | 张文龙 | Tea saponin EW (emulsion in water) compound plant source organic insecticide and preparation method thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004115410A (en) * | 2002-09-25 | 2004-04-15 | Ichimaru Pharcos Co Ltd | Foaming agent or cosmetic composition |
| US6733802B1 (en) * | 1997-12-17 | 2004-05-11 | Fortune Bio-Tech Limited | Natural azadirachtin composition |
| DE102007004500A1 (en) * | 2007-01-30 | 2008-07-31 | Seibicke, Tobias, Dr. | Use of a herbal extract containing formulation for prophylactic and/or therapeutic treatment of plant diseases, which is supported or caused by pathogens, where the formulation contains extract of plants or its parts of Sapindus |
| JP2010126524A (en) * | 2008-12-01 | 2010-06-10 | Maruzen Pharmaceut Co Ltd | Plant disease control agent of vegetable origin |
| US20100291241A1 (en) * | 2009-05-15 | 2010-11-18 | Jaime Toro Restrepo | METHOD OF PRODUCTION OF EXTRACT DERIVED FROM Swinglea Glutinosa LEAVES |
| US20110059195A1 (en) * | 2009-05-15 | 2011-03-10 | Jamez Alberto Jimenez Martinez | Swinglea glutinosa leaves derived material in combination with pyrethroids for control of acari |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101069544B (en) * | 2007-06-12 | 2010-10-06 | 贵州神奇集团控股有限公司 | Milk-honey fruit-jelly making method |
| JP2010018544A (en) * | 2008-07-10 | 2010-01-28 | Nippon Zettoc Co Ltd | Antimicrobial agent, composition for oral cavity and denture cleanser |
-
2010
- 2010-05-10 US US12/776,511 patent/US8298590B2/en not_active Expired - Fee Related
-
2011
- 2011-05-05 BR BR112012028716A patent/BR112012028716A2/en not_active IP Right Cessation
- 2011-05-05 WO PCT/IB2011/000964 patent/WO2011141788A1/en active Application Filing
- 2011-05-05 EP EP11780277.7A patent/EP2568813A4/en not_active Withdrawn
- 2011-05-05 AU AU2011251744A patent/AU2011251744A1/en not_active Abandoned
- 2011-05-10 UY UY0001033374A patent/UY33374A/en not_active Application Discontinuation
- 2011-05-10 AR ARP110101618A patent/AR083149A1/en unknown
-
2012
- 2012-07-11 US US13/547,027 patent/US8337912B2/en not_active Expired - Fee Related
- 2012-12-10 CO CO12223284A patent/CO6602114A2/en not_active Application Discontinuation
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6733802B1 (en) * | 1997-12-17 | 2004-05-11 | Fortune Bio-Tech Limited | Natural azadirachtin composition |
| JP2004115410A (en) * | 2002-09-25 | 2004-04-15 | Ichimaru Pharcos Co Ltd | Foaming agent or cosmetic composition |
| DE102007004500A1 (en) * | 2007-01-30 | 2008-07-31 | Seibicke, Tobias, Dr. | Use of a herbal extract containing formulation for prophylactic and/or therapeutic treatment of plant diseases, which is supported or caused by pathogens, where the formulation contains extract of plants or its parts of Sapindus |
| JP2010126524A (en) * | 2008-12-01 | 2010-06-10 | Maruzen Pharmaceut Co Ltd | Plant disease control agent of vegetable origin |
| US20100291241A1 (en) * | 2009-05-15 | 2010-11-18 | Jaime Toro Restrepo | METHOD OF PRODUCTION OF EXTRACT DERIVED FROM Swinglea Glutinosa LEAVES |
| US20110059195A1 (en) * | 2009-05-15 | 2011-03-10 | Jamez Alberto Jimenez Martinez | Swinglea glutinosa leaves derived material in combination with pyrethroids for control of acari |
Non-Patent Citations (1)
| Title |
|---|
| See also references of EP2568813A4 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103444805A (en) * | 2013-09-10 | 2013-12-18 | 苏州市丰澄农业专业合作社 | Ilex pubescens plant based nano slow-release suspending agent |
Also Published As
| Publication number | Publication date |
|---|---|
| AR083149A1 (en) | 2013-02-06 |
| EP2568813A4 (en) | 2015-01-07 |
| UY33374A (en) | 2011-12-30 |
| US20110274777A1 (en) | 2011-11-10 |
| EP2568813A1 (en) | 2013-03-20 |
| US8337912B2 (en) | 2012-12-25 |
| AU2011251744A1 (en) | 2013-01-10 |
| BR112012028716A2 (en) | 2018-05-15 |
| CO6602114A2 (en) | 2013-01-18 |
| US8298590B2 (en) | 2012-10-30 |
| US20120276230A1 (en) | 2012-11-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| de Rodríguez et al. | Antifungal activity in vitro of Rhus muelleri against Fusarium oxysporum f. sp. lycopersici | |
| Suman et al. | GC–MS analysis of bioactive components and synthesis of silver nanoparticle using Ammannia baccifera aerial extract and its larvicidal activity against malaria and filariasis vectors | |
| de Rodríguez et al. | Antifungal activity in vitro of ethanol and aqueous extracts of leaves and branches of Flourensia spp. against postharvest fungi | |
| Louail et al. | Antimicrobial and antioxidant activity of essential oil of Ammodaucus leucotrichus Coss. & Dur. seeds | |
| US20200037615A1 (en) | Method of production of extract derived from swinglea glutinosa leaves | |
| CN109601559B (en) | Wild chrysanthemum essential oil for preventing and treating phytophthora and application thereof | |
| Noureddine et al. | Evaluation of erythrocytes toxicity and antioxidant activity of alkaloids of Fumaria capreolata | |
| US8337912B2 (en) | Method for extraction of material from a Sapindacea family fruit | |
| Kannan et al. | Antidiabetic and antioxidant properties of Waltheria indica L., an ethnomedicinal plant | |
| Gursoy et al. | Determination of in vitro antioxidative and antimicrobial properties and total phenolic contents of Ziziphora clinopodioides, Cyclotrichium niveum, and Mentha longifolia ssp. typhoides var. typhoides | |
| Aly et al. | Antimicrobial qctivities and phytochemical analysis of the essential oil of Lavandula dentata and Plectranthus tenuiflorus, collected from Al Baha region, Saudi Arabia | |
| Elsherbiny et al. | Fungitoxicity of organic extracts of Ocimum basilicum on growth and morphogenesis of Bipolaris species (teleomorph Cochliobolus) | |
| Kadébé et al. | Antidiabetic activity of Plumeria alba Linn (apocynaceae) root extract and fractions in streptozotocin-induced diabetic rats | |
| Lakhdar et al. | Phytochemical analysis and antifungal activity of Anvillea radiata | |
| Ahmad et al. | Evaluation of antioxidant potential of medicinal plants from Malaysian Rubiaceae (subfamily Rubioideae) | |
| Maghsoodi et al. | Efficacy of Althaea officinalis leaf extract in controlling Alternaria spp. pathogenic on Citrus | |
| Tham et al. | Influence of different extraction temperatures and methanol solvent percentages on the total phenols and total flavonoids from the heartwood and bark of Acacia auriculiformis | |
| Nermien et al. | In vitro-exploration of fungal endophytes of Egyptian Cynara scolymus L.(artichoke) and investigation of some their bioactive potentials | |
| Manikandan et al. | In vitro antioxidant activity of extracts of Psidium guajava leaves | |
| Eboh et al. | Metabolites of Colletrotricum species, an endophytic fungus isolated from Vernonia amygdalina Del possess antimicrobial and antioxidant activities | |
| LAKHDAR et al. | Bubonium graveolens extracts for controlling Fusarium oxysporum f. sp. albedinis | |
| Lawal et al. | Gas Chromatography-Mass Spectrometry and Cytotoxicity of Securidaca longepedunculata (polygalaceae) Root Bark Extract | |
| Dehghani Bidgoli et al. | Essential Oil Chemical Composition, Antioxidant and Antibacterial Activities of Eucalyptus largiflorens F. Muell | |
| Nikkitha et al. | Bioactive metabolites from ethyl acetate extract of leaves of Melia dubia L., against human and plant microbial pathogens | |
| Shekhawat et al. | Identification of Bacterial Endophytes Isolated From Different Medicinal Plants. |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 11780277 Country of ref document: EP Kind code of ref document: A1 |
|
| NENP | Non-entry into the national phase |
Ref country code: DE |
|
| WWE | Wipo information: entry into national phase |
Ref document number: 2011780277 Country of ref document: EP |
|
| WWE | Wipo information: entry into national phase |
Ref document number: 10663/DELNP/2012 Country of ref document: IN |
|
| WWE | Wipo information: entry into national phase |
Ref document number: 12223284 Country of ref document: CO |
|
| REG | Reference to national code |
Ref country code: BR Ref legal event code: B01A Ref document number: 112012028716 Country of ref document: BR |
|
| ENP | Entry into the national phase |
Ref document number: 2011251744 Country of ref document: AU Date of ref document: 20110505 Kind code of ref document: A |
|
| ENP | Entry into the national phase |
Ref document number: 112012028716 Country of ref document: BR Kind code of ref document: A2 Effective date: 20121109 |