WO2011138228A1 - Aqueous composition for ophthalmic or dermal use - Google Patents

Aqueous composition for ophthalmic or dermal use Download PDF

Info

Publication number
WO2011138228A1
WO2011138228A1 PCT/EP2011/056753 EP2011056753W WO2011138228A1 WO 2011138228 A1 WO2011138228 A1 WO 2011138228A1 EP 2011056753 W EP2011056753 W EP 2011056753W WO 2011138228 A1 WO2011138228 A1 WO 2011138228A1
Authority
WO
WIPO (PCT)
Prior art keywords
composition
component
weight
solution
hyaluronan
Prior art date
Application number
PCT/EP2011/056753
Other languages
French (fr)
Inventor
Klaus Naserke
Alessandro Di Napoli
Original Assignee
Trb Chemedica Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Trb Chemedica Ag filed Critical Trb Chemedica Ag
Publication of WO2011138228A1 publication Critical patent/WO2011138228A1/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/24—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing atoms other than carbon, hydrogen, oxygen, halogen, nitrogen or sulfur, e.g. cyclomethicone or phospholipids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/66—Phosphorus compounds
    • A61K31/683—Diesters of a phosphorus acid with two hydroxy compounds, e.g. phosphatidylinositols
    • A61K31/685—Diesters of a phosphorus acid with two hydroxy compounds, e.g. phosphatidylinositols one of the hydroxy compounds having nitrogen atoms, e.g. phosphatidylserine, lecithin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • A61K31/726—Glycosaminoglycans, i.e. mucopolysaccharides
    • A61K31/728—Hyaluronic acid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
    • A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/55—Phosphorus compounds
    • A61K8/553—Phospholipids, e.g. lecithin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
    • A61K8/73—Polysaccharides
    • A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0014—Skin, i.e. galenical aspects of topical compositions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0048—Eye, e.g. artificial tears
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/107—Emulsions ; Emulsion preconcentrates; Micelles
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P27/00—Drugs for disorders of the senses
    • A61P27/02—Ophthalmic agents
    • A61P27/04—Artificial tears; Irrigation solutions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin

Definitions

  • the present invention relates to aqueous compositions for dermal or ophthalmic use.
  • the invention relates to formulations comprising a phospholipid component and a hyaluronan component which are useful, for example, for pharmaceutical and cosmetic applications, including the treatment, prevention or alleviation of symptoms of diseases or conditions of the skin or the eye, such as ocular dryness.
  • Tear fluid is a filtrate of blood, devoid of cells, which is secreted to the ocular surface by the lacrimal gland and enriched with mucinous components from the goblet cells in the upper lid and a protecting film of lipids formed by the meibomian glands.
  • the resulting fluid of approximately 10 ⁇ thickness is continuously spread over the precorneal surface through permanent blinks of the upper eyelid.
  • the three layers of this tear film consist from a mucin layer attached to the corneal surface, an aqueous layer and an outer lipid layer preventing the evaporation of the aqueous component to the ambient and, thus, the rupture and dysfunction of the tear film.
  • the lipid film consists of a multitude of components including phospholipids, sphingolipids, ceramides, cerebrosides, wax esters, cholesterol esters, triglycerides and free fatty acids.
  • the phospholipid fraction represents about 1-5 % of the total lipid secretion, with the main components being phosphatidylcholine (PC) with a percentage close to 40 % of the total phospholipids and phosphatidylethanolamin (PE) with a percentage of about 18 %.
  • PC phosphatidylcholine
  • PE phosphatidylethanolamin
  • the aqueous layer of the tear film also comprises numerous components, mainly water, proteins and salts.
  • the proteins include mucins, immunoglobulins, lysozyme, lipocalins, lactoferrin and lacritin.
  • the function of the tear film is that of keeping the ocular surface wet, protecting the corneal and conjunctival epithelium and transporting biologically active substances which are useful for the physiology of the eye, such as, for example, nutrients and oxygen.
  • biologically active substances which are useful for the physiology of the eye, such as, for example, nutrients and oxygen.
  • the tear film must have a certain surface tension so that the film can be extended over the epithelium and it must have a physiological evaporation speed.
  • the usual treatment of dry eye consists of relieving the symptoms by applying tear replacements topically.
  • Typical compositions of these preparations include polymeric solutions which, due their viscosity, reduce the evaporation of the tear film.
  • hypo-osmolar formulations aimed to counteract the hyperosmolarity of tear fluid in evaporative dry eye and, at the same time, ions/salts like potassium, calcium or magnesium which are essential for the integrity and function of cells on the corneal surface.
  • compositions and formulations suffer from some significant disadvantages such as offering little relief to dry eyes or requiring preservation at low temperatures due to the poor stability of the lipid components at room temperature.
  • an artificial tear composition that has excellent lubrication properties, reduces fluid evaporation to maintain the tear film, regulates the homeostasis of water and ions, has a sufficient surface tension to allow extension over the epithelial surface, provides a surface preventing adhesion of the ocular surface to a contact lens or eye lid and/or provides a biological barrier to pathogens such as bacteria or viruses.
  • an aqueous composition that provides or supports important structures and functions of the tear fluid by comprising a mucin component (like hyaluronan) anchoring on the corneal surface, an aqueous phase containing ions essential for cell homeostasis, the aqueous phase also balancing tear hyperosmolarity by hypoosmolar formulation, and a lipid layer (like phospholipids) preventing evaporation of the aqueous phase is particularly suited to meet the above- described need.
  • a mucin component like hyaluronan
  • an aqueous composition comprising a phospholipid component and a hyaluronan component and, optionally, a stabilizing component has improved properties compared to known formulations and is useful for ophthalmic and dermal applications, such as the treatment, prevention or alleviation of the symptoms of dry eyes and other conditions of the eye, such as sore eyes or conjunctivitis.
  • Other applications include the use as a lubricant for contact lenses or intra-ocular lenses, the use as a carrier for pharmaceuticals, or cosmetic applications.
  • the present invention is thus directed to an aqueous composition
  • a phospholipid component and a hyaluronan component.
  • the ophthalmically suitable carrier or diluent is water.
  • the composition is hypoosmolar relative to a bodily fluid or a body fluid compartment, such as tear fluid or the eye.
  • the osmolarity of such a composition may, for example, be ⁇ 300 mOsmoI/kg, preferably ⁇ 250 mOsmol/kg, more preferably ⁇ 200 or about 150 mOsmol/kg.
  • the composition further comprises a stabilizing component.
  • the stabilizer may for example be urea.
  • the composition further comprises ions or salts which are essential for the homeostasis of cells on the corneal surface.
  • ions may include alkaline metal or alkaline earth metal ions, including, but not limited to sodium, potassium, calcium and magnesium ions. In preferred embodiments, these ions are selected from potassium, calcium and magnesium and combinations thereof.
  • the afore-mentioned ions may be provided in form of an inorganic salt.
  • the composition further comprises auxiliaries, such as antioxidants, surfactants, preservatives, natural or synthetic oil components, alcohols, thickening agents, vitamins and inorganic salts or combinations thereof.
  • auxiliaries such as antioxidants, surfactants, preservatives, natural or synthetic oil components, alcohols, thickening agents, vitamins and inorganic salts or combinations thereof.
  • the auxiliaries may, for example, comprise an alcohol.
  • the composition is adapted for ophthalmic or dermal use. This use may be therapeutic or cosmetic.
  • the present invention is directed to the use of the inventive composition for the manufacture of a medicinal product or medical device for treating, preventing or alleviating a condition of the eye or the skin in a subject in need thereof
  • This subject may for example be an animal, such as a mammal, preferably a human being.
  • the present invention is directed to a method for the treatment, prevention or alleviation of a condition of the eye or the skin, comprising administering a composition according to the invention to a subject in need thereof.
  • the present invention also encompasses a process for
  • composition according to the invention comprising:
  • the present invention also relates to a process for the preparation of a composition according to the invention, comprising:
  • the present invention generally relates to a formulation that is suitable as a replacement for the precorneal tear film and/or that can restore the precorneal tear film.
  • compositions of the invention include the use as a carrier medium for the delivery of drugs, the use as a lubricant for ocular lenses, the use as a component of cosmetic formulations for eye or skin cosmetics.
  • the compositions of the invention may also be suited for dermal applications, such as the treatment or prevention of dry skin or other conditions of the skin.
  • compositions of the invention are aqueous compositions that comprise a phospholipid component and a hyaluronan component.
  • Aqueous as used herein means that the composition is water-based, i.e. that it includes water as a carrier or diluent.
  • the composition comprises about 0.01 to about 5 % by weight, about 0.1 to about 3 % by weight, or about 1 % by weight of the phospholipid component.
  • the composition comprises about 0.01 to about 1 % by weight, about 0.05 to about 0.5 % by weight, or about 0.1 % by weight of the hyaluronan component.
  • the ratio of the phospholipid component and the hyaluronan component may be in the range of about 50: 1 to about 1:1, about 20: 1 to about 5:1, or about 10:1 by weight.
  • the composition further comprises a stabilizing component.
  • the stabilizing component increases the stability of the phospholipids and prevents the formulation from becoming rancid.
  • the composition may comprise about 0.01 to about 1 % by weight, about 0.1 to about 0.5 % by weight, or about 0.16 % by weight of the stabilizing component.
  • stabilizing component and “stabilizer” are used interchangeably herein and relate to compounds or compositions that increase the stability of the formulation, Le. prevent degradation of the phospholipid and/or hyaluronan component.
  • the stabilizer prevents degradation of the lipids and thus prevents the composition from becoming rancid.
  • the use of a stabilizer may prolong the shelf-life of the composition and may also allow storage at room temperature without compromising the structural integrity of the contained lipids.
  • the stabilizing component is selected from the group of urea and carbohydrates.
  • the carbohydrates are monosaccharides or derivatives thereof, such as sugar alcohols or amino sugars.
  • the carbohydrates may, for example, be selected from the group consisting of glucose, fructose, mannose, galactose, sorbitol, mannitol and inositol.
  • the phospholipid component may comprise, consist essentially of, or consist of (i) phosphoglycerides, such as phosphatidic acids, cytidylic phosphoglycerides (CDP diglycerides), phosphatidylcholines, phosphatidylethanolamines, phosphatidylserine, phosphatidyl-N- methylethanolamine, phosphatidyl-N,N-dimethylethanolamine, phosphatidyl-N- acylethanolamine, phosphatidyI-N-2-hydroxyethylalanine, phosphatidylglycerol, phosphatidylglycerophosphate, diphosphatidylglycerol, lysobisphosphatidic acids, phosphatidylglucosaminylglycerol, phosphatidylinositol, phosphatidylinositol monophosphate and diphosphate,
  • phosphoglycerides such as
  • the phospholipid component comprises, consists essentially of, or consists of phosphatidylcholine (PC).
  • PC phosphatidylcholine
  • the phosphatidylcholine may be fully saturated and may for example comprise or consist of dipalmitoyl- phosphatidylcholine (DPPC), distearoyl-phosphatidylcholine (DSPC) or mixtures thereof.
  • DPPC dipalmitoyl- phosphatidylcholine
  • DSPC distearoyl-phosphatidylcholine
  • Full saturation of the fatty acid residues may be achieved by appropriate chemical treatment of the PC, for example hydration.
  • the phosphatidylcholine is of natural or synthetic origin.
  • the hyaluronan component may be selected from hyaluronic acid, hyaluronic acids esters and hyaluronic acid salts or combinations thereof.
  • the hyaluronic acid salts may be selected from the group consisting of sodium hyaluronate, potassium hyaluronate, iron hyaluronate, calcium hyaluronate, magnesium hyaluronate and zinc hyaluronan or combinations thereof.
  • the molecules of the hyaluronan component have a mean molecular weight of about 100,000 to about 2,500,000 Da, about 100,000 to about
  • the composition of of the invention is hypoosmolar relative to a bodily fluid or body fluid compartment.
  • Body fluid as used herein relates to fluids natural occuring in the human or animal body and include, but are not limited to, blood, plasma, serum, lymph, cerebrospinal fluid, saliva, tear fluid, urine, sweat, and vaginal secretion.
  • Body fluid compartment as used herein relates to a fluid compartment of the body and includes intracellular fluid and extracellular fluid, with the latter being subdivided into the extravascular compartment comprising interstitial fluid and the intravascular compartment comprising mainly blood. Usually these compartments are in osmotic equilibrium. The normal osmolarity of body fluids lies in the range of 280-320 mOsmol/1 H 2 0. Isotonic saline (0.9% NaCl) has an osmolarity of 308 mOsmol/1.
  • the osmolarity of the composition is ⁇ 300 mOsmol/kg, ⁇ 250 mOsmol/kg, ⁇ 200 mOsmol/kg or about 150 mOsmol kg H 2 0.
  • the osmolarity may be controlled by a tonicity adjusting agent, such as the inorganic salts mentioned below, glycerol, sorbitol, mannitol, ethylene glycol, propylene glycol, and dextrose.
  • compositions of the invention may be pharmaceutical or cosmetic compositions and may be for ophthalmic or dermal use.
  • the composition may fiirther comprise ions or salts.
  • the salts may, for example, be inorganic salts.
  • the ions may be, for example, alkaline metal or earth alkaline metal ions, including, but not limited to sodium, potassium, calcium and magnesium or combinations thereof.
  • the salts are selected from sodium chloride, potassium chloride, monosodium phosphate, disodium phosphate, sodium citrate, magnesium chloride, and calcium chloride or mixtures thereof.
  • the ions or salts are essential for the homeostasis of cells on the corneal surface and comprise one or more ions selected from potassium, calcium and magnesium. These ions may be provided in form of salts.
  • one or more of these ions are provided in form of citrate salts.
  • citrate as an anion in these salts is preferred, because citrate acts as a chelating agent for calcium and magnesium ions and thus prevents the formation of insoluble salts of magnesium and calcium ions with the hyaluronan component.
  • citrate ions may be provided separately, for example in form of sodium citrate.
  • the concentrations of these ions are about 0.001 to about 0.1 % by weight of the formulation.
  • the potassium concentration is about 0.055 % by weight
  • the magnesium concentration is about 0.0015 % by weight
  • the calcium concentration is about 0.005 % by weight.
  • the composition may further comprise one or more auxiliaries, carriers or diluents.
  • the additional diluents may, for example, comprise propylene glycol.
  • the auxiliaries may be selected from the group consisting of antioxidants, surfactants, buffering agents, preservatives, natural or synthetic oil components, alcohols, thickening agents, vitamins and inorganic salts or combinations thereof.
  • the inorganic salts include, but are not limited to sodium chloride.
  • the composition may comprise sodium chloride, for example in a concentration of about 0.01 to about 2 % by weight or about 0.1 to about 1 % by weight of the composition.
  • the composition may comprise an antioxidant, such as a tocopherol, tocotrienol, tocomonoenol or marine tocopherol (MDT).
  • the antioxidant may thus be selected from the group consisting of alpha-, beta-, gamma-, delta- tocopherol, alpha-, beta-, gamma-, delta-tocotrienol, alpha-, beta-, gamma-, delta- tocomonoenol, alpha-, beta-, gamma-, and delta-MDT and mixtures thereof.
  • the inventived compositions comprise a natural or synthetic oil component selected from the group consisting of wax esters, mineral oils, synthetic hydrocarbons, silicon oils, natural and synthetic di- and triglycerides, and fatty acids.
  • the composition comprises one or more alcohols selected from the group consisting of ethanol, propanol, isopropanol, 1,2- propyleneglycol, 1,3-butyleneglycol, glycerol or mixtures thereof.
  • Preservatives useful for the compositions of the invention are those that are non-irritating to the skin or the eye and which are compatible with the components of the composition. Suitable preservatives include but are not limited to sorbic acid and ethylenediamine tetraacetic acid (EDTA). The preservative may be used in a biostatic amount that prevents contamination of the composition. The preservative may include polyhexanide.
  • composition includes surfactants, these are preferyl non-ionic. Suitable examples include polysorbate, block copolymers of ethylene oxide and propylene oxide (Pluronic®), and polyethoxylated castor oil (Cremophor®).
  • Buffering agents that may be used in the invented compositions include, but are not limited to Tris (Tris(hydroxymethyl)aminomethane), NaOH, histidine, tricine, lysine, glycine and serine adjusted to the correct pH with an acidic component with low ionic force.
  • the compositions may also comprise further auxiliaries such as hypromellose (hydroxypropylmethylcellulose), chitosan, lubricin, xanthan gum, parabens, benzalkonium chloride, polyhexamethylene biguanide, arginine, glycine, heparin, sodium pentosan polysuifate and/or lysine.
  • the invented compositions may have a pH in the range of from about 6 to about 8, about 7 or about 7.3.
  • compositions of the invention further comprise a pharmaceutically active component.
  • the pharmaceutically active component may be selected from the group consisting of antibiotics, antifungals, analgesics, anesthetics, anti-allergics, and anti- inflammatory agents.
  • the pharmaceutical may also be useful for the treatment of conditions of the eye, such as glaucoma, conjunctivitis, or other inflammatory conditions.
  • the composition of the invention comprises, consists essentially of or consists of: 0.1 % by weight sodium hyaluronate, 1 % by weight phosphatidylcholine and 0.16 % by weight urea.
  • the composition may further comprise: about 0.28 % by weight sodium chloride, about 0.1 % by weight potassium chloride, about 0.32 % by weight disodium phosphate dodecahydrate, about 0.026 % by weight sodium citrate, about 0.01 % by weight magnesium chloride hexahydrate, about 0.01 % by weight calcium chloride dehydrate, and about 5 % by weight glycerol.
  • the composition may further comprise water to add up to 100 %.
  • the afore-mentioned composition may have a pH of about 7.3.
  • compositions can be in any form that allows application or administration, including, but not limited to a spray, dispersion, cream, gel or solution.
  • the composition may be adapted for topical application.
  • the composition is in single unit dose or multiple unit dose form.
  • the compositions are in a ready to use format. In other embodiments, they can form part of a kit comprising a concentrated form of the composition that has to be diluted with a suitable diluent before use.
  • the diluent may, for example, be water.
  • the diluent may also be contained in the kit. In such a kit, the components of the composition can be contained in separate containers and be combined before use.
  • the compositions of the invention may be used for treating, preventing or alleviating a condition of the eye or the skin in a subject or patient in need thereof. This use may involve the manufacture of a pharmaceutical for such use.
  • the condition of the eye treated, prevented or alleviated may be ocular dryness, dry eye syndrome, ocular discomfort, or Sjogren syndrome.
  • the subject may be a mammal, preferably a human being.
  • compositions of the invention may also be used for inhibiting the evaporation of the aqueous layer of the tear film.
  • compositions are as vehicles for ophthalmic or dermal medications.
  • the compositions serve as a carrier solution in which the drug to be delivered is solved, disperged, or emulgated.
  • compositions of the invention may be used for the manufacture of a cosmetic product.
  • a further use of the invented compositions is as a lubricant for ocular lenses, such as contact lenses.
  • the invention also encompasses a method for the treatment, prevention or alleviation of a condition of the eye or the skin, wherein this method comprises administering a composition of the invention to a subject in need thereof.
  • the condition of the eye may be ocular dryness, dry eye syndrome, ocular discomfort, or Sjogren syndrome.
  • the subject may be a mammal, preferably a human being.
  • compositions of the invention may be prepared according to a process comprising:
  • the composition may be prepared by a process comprising: (a) preparing an aqueous solution comprising the phospholipid component and, if present, the stabilizer, salt and/or alcohol component;
  • preparation of the aqueous solution may be carried out at elevated temperature, for example at about 50 to about 90 °C.
  • compositions of the present invention impart excellent lubrication at low load due to their content of hyaluronan. At points of high load they provide excellent boundary lubrication due to formation of an oligolamellar layer of phospholipid.
  • the present compositions also have anti-adhesive properties due to their phospholipid content. They enhance the retention of fluid by the tear film by retaining water molecules that are associated with the long hydrophilic hyaluronic acid molecules and by providing a phospholipid monolayer reducing evaporation at the air-aqueous interface.
  • the composition of the present invention also reduces film rupture by reducing the surface energies of both the tissue-liquid and air-liquid interfaces. The latter can be reduced by hyaluronic acid acting as a wetting acid, while the former can be reduced by locating a phospholipid monolayer on the surface of the tear film to reduce surface tension.
  • Solution 1 and Solution 2 were prepared separately and then combined.
  • Solution I was prepared according to the following process:
  • a cleaned, stainless steel mixing tank (size: 100L) equipped with a stirrer was washed with steam (123°C to 138°C) for 30 minutes.
  • the mixing tank number and the time were recorded.
  • About 80% of the total amount of water for injections was set in the mixing tank.
  • Temperature of the water for injections 25°C ⁇ 5°C.
  • the following substances were added one by one while stirring: sodium chloride, urea, glycerol and phosphatidycholine. These substances must be dissolved before any more of the substances are added.
  • Stirring time was at least 15 minutes at 350 ⁇ 50 rpm. If necessary, the pH value was adjusted to 7.2-7.4 with hydrochloric acid 10% or sodium hydroxide 4% solution.
  • Solution 2 was prepared according to the following process:
  • a cleaned, stainless steel mixing tank (size: 100L) equipped with a stirrer was washed with steam (123°C to 138°C) for 30 minutes.
  • the mixing tank number and the time were recorded.
  • About 80% of the total amount of water for injections was set in the mixing tank.
  • Temperature of the water for injections 25°C + 5°C.
  • the following substances were added one by one while stirring: sodium citrate, sodium chloride, potassium chloride and sodium phosphate dodecahydrate. These substances must be dissolved before any more of the substances are added.
  • Stirring time was at least 15 minutes at 350 ⁇ 50 rpm. If necessary, the pH value was adjusted to 7.2-7.4 with hydrochloric acid 10% or sodium hydroxide 4% solution.
  • Stirring time was at least 15 minutes after each adding at 350 ⁇ 50 rpm.
  • the amount of hydrochloric acid 10% or sodium hydroxide 4% solution used was recorded. Under stirring, the following substances were then added and dissolved: calcium chloride dihydrate and magnesium chloride hexahydrate.
  • Stirring time was at least 15 minutes at 350 ⁇ 50 rpm. Under slow stirring at 350 ⁇ 50 rpm sodium hyaluronate was added and the stirring continued for at least 12 hours until complete dissolution. Care was taken to avoid lump formation.
  • the pH (7.2-7.4) was checked, and, if necessary, corrected with hydrochloric acid 10% or 4% sodium hydroxide solution.
  • Stirring time was at least 5 minutes after each adding at 350 ⁇ 50 rpm.
  • Solution 1 (sterile by moist heat) was step by step unified with solution 2 (sterile by filtration) in the mixing tank already containing solution 2. Stirring time was at least 15 minutes at 350 ⁇ 50 rpm after having added the full quantity of solution 1. Finally, the resulting emulsion was aseptically filled into adequate monodose or multidose containers.
  • Table 3 shows a formulation with lower concentrations of sodium hyaluronate and phosphatidylcholine, which in addition contains lubricin
  • Table 4 shows a formulation with lower concentrations of Sodium hyaluronate and phosphatidylcholine, which in addition contains chitosan
  • Table 5 shows a formulation which is sterile by the addition of Cosmocil (Polyhexanide) Table 5

Abstract

The present invention relates to aqueous compositions comprising a phospholipid component and a hyaluronan component and, optionally, a stabilizer, which are useful for pharmaceutical and cosmetic applications, including the treatment, prevention or alleviation of symptoms of diseases or conditions of the skin or the eye, such as ocular dryness. The invention further relates to the use of such compositions and processes for their production.

Description

Aqueous composition for ophthalmic or dermal use
Field of the Invention
[0001] The present invention relates to aqueous compositions for dermal or ophthalmic use. In particular, the invention relates to formulations comprising a phospholipid component and a hyaluronan component which are useful, for example, for pharmaceutical and cosmetic applications, including the treatment, prevention or alleviation of symptoms of diseases or conditions of the skin or the eye, such as ocular dryness.
Background
[0002] The ocular surface is coated by the so-called precorneal film or tear film which is protecting and nourishing the avascular corneal surface. In addition, the tear film contributes to the visual performance of the eye. Tear fluid is a filtrate of blood, devoid of cells, which is secreted to the ocular surface by the lacrimal gland and enriched with mucinous components from the goblet cells in the upper lid and a protecting film of lipids formed by the meibomian glands. The resulting fluid of approximately 10 μιη thickness is continuously spread over the precorneal surface through permanent blinks of the upper eyelid. The three layers of this tear film consist from a mucin layer attached to the corneal surface, an aqueous layer and an outer lipid layer preventing the evaporation of the aqueous component to the ambient and, thus, the rupture and dysfunction of the tear film.
[0003] The lipid film consists of a multitude of components including phospholipids, sphingolipids, ceramides, cerebrosides, wax esters, cholesterol esters, triglycerides and free fatty acids.
[0004] The phospholipid fraction represents about 1-5 % of the total lipid secretion, with the main components being phosphatidylcholine (PC) with a percentage close to 40 % of the total phospholipids and phosphatidylethanolamin (PE) with a percentage of about 18 %.
[0005] It has been hypothesized that this fraction produces a reduction in the surface tension of the aqueous face and facilitates the extensibility of the precorneal film during the blinking movement. [0006] The aqueous layer of the tear film also comprises numerous components, mainly water, proteins and salts. The proteins include mucins, immunoglobulins, lysozyme, lipocalins, lactoferrin and lacritin.
[0007] The function of the tear film is that of keeping the ocular surface wet, protecting the corneal and conjunctival epithelium and transporting biologically active substances which are useful for the physiology of the eye, such as, for example, nutrients and oxygen. To preserve the physical characteristics of the ocular epithelium, the tear film must have a certain surface tension so that the film can be extended over the epithelium and it must have a physiological evaporation speed.
[0008] An imbalance in the composition of the tear film leads to impairment or loss of the capability to coat the ocular surface and withstand rapid evaporation and thus determines the rise of conditions of the eye, such as ocular dryness and dry eye syndrome.
[0009] One cause of the onset of ocular dryness and in particular the dry eye syndrome is the excessive evaporation of the aqueous phase of the tear film caused by an imbalance in the outer lipid layer following qualitative and/or quantitative modifications of its composition. A major consequence of excessive evaporation of water is an increase in the concentration of ions/salts dissolved in the aqueous phase of the tear fluid, leading to hyperosmolarity which may reach 350 mMol/L.
[0010] The usual treatment of dry eye consists of relieving the symptoms by applying tear replacements topically. Typical compositions of these preparations include polymeric solutions which, due their viscosity, reduce the evaporation of the tear film.
[0011] Other formulations based on phospholipids and/or oil components, optionally as oil-in-water emulsions or in form of liposomes, are used to restore the natural lipid layer of the tear film.
[0012] A few products only are providing hypo-osmolar formulations, aimed to counteract the hyperosmolarity of tear fluid in evaporative dry eye and, at the same time, ions/salts like potassium, calcium or magnesium which are essential for the integrity and function of cells on the corneal surface.
[0013] However, all known compositions and formulations suffer from some significant disadvantages such as offering little relief to dry eyes or requiring preservation at low temperatures due to the poor stability of the lipid components at room temperature.
[0014] Therefore, there is need for an artificial tear composition that has excellent lubrication properties, reduces fluid evaporation to maintain the tear film, regulates the homeostasis of water and ions, has a sufficient surface tension to allow extension over the epithelial surface, provides a surface preventing adhesion of the ocular surface to a contact lens or eye lid and/or provides a biological barrier to pathogens such as bacteria or viruses. Summary of the Invention
[0015] It is one object of the present invention to provide a composition that meets this long-felt need.
[0016] It has now surprisingly be found that an aqueous composition that provides or supports important structures and functions of the tear fluid by comprising a mucin component (like hyaluronan) anchoring on the corneal surface, an aqueous phase containing ions essential for cell homeostasis, the aqueous phase also balancing tear hyperosmolarity by hypoosmolar formulation, and a lipid layer (like phospholipids) preventing evaporation of the aqueous phase is particularly suited to meet the above- described need.
[0017] Furthermore, it has been found that an aqueous composition comprising a phospholipid component and a hyaluronan component and, optionally, a stabilizing component has improved properties compared to known formulations and is useful for ophthalmic and dermal applications, such as the treatment, prevention or alleviation of the symptoms of dry eyes and other conditions of the eye, such as sore eyes or conjunctivitis. Other applications include the use as a lubricant for contact lenses or intra-ocular lenses, the use as a carrier for pharmaceuticals, or cosmetic applications.
[0018] In a first aspect the present invention is thus directed to an aqueous composition comprising a phospholipid component and a hyaluronan component. In such a composition, the ophthalmically suitable carrier or diluent is water.
[0019] In certain embodiments of the invented composition, the composition is hypoosmolar relative to a bodily fluid or a body fluid compartment, such as tear fluid or the eye. The osmolarity of such a composition may, for example, be < 300 mOsmoI/kg, preferably < 250 mOsmol/kg, more preferably < 200 or about 150 mOsmol/kg.
[0020] In other embodiments of the invention, the composition further comprises a stabilizing component. The stabilizer may for example be urea.
[0021] In other embodiments of the invention, the composition further comprises ions or salts which are essential for the homeostasis of cells on the corneal surface. Such ions may include alkaline metal or alkaline earth metal ions, including, but not limited to sodium, potassium, calcium and magnesium ions. In preferred embodiments, these ions are selected from potassium, calcium and magnesium and combinations thereof. The afore-mentioned ions may be provided in form of an inorganic salt.
[0022] In certain embodiments of the invention, the composition further comprises auxiliaries, such as antioxidants, surfactants, preservatives, natural or synthetic oil components, alcohols, thickening agents, vitamins and inorganic salts or combinations thereof. The auxiliaries may, for example, comprise an alcohol.
[0023] In specific embodiments, the composition is adapted for ophthalmic or dermal use. This use may be therapeutic or cosmetic.
[0024] In a further aspect, the present invention is directed to the use of the inventive composition for the manufacture of a medicinal product or medical device for treating, preventing or alleviating a condition of the eye or the skin in a subject in need thereof This subject may for example be an animal, such as a mammal, preferably a human being.
[0025] In another aspect, the present invention is directed to a method for the treatment, prevention or alleviation of a condition of the eye or the skin, comprising administering a composition according to the invention to a subject in need thereof.
[0026] In still another aspect, the present invention also encompasses a process for
[0027] the preparation of a composition according to the invention, comprising:
(a) sterilizing the phospolipid component by treatment with gamma-radiation or moist heat;
(b) preparing an aqueous solution comprising the hyaluronan component and, if present, the stabilizer, salt and/or alcohol component;
(c) adjusting the pH of the solution of (b);
(d) sterilizing the solution of (c) by sterile filtration; and (e) combining the phospholipids of (a) and the aqueous solution (d) by homogenization under sterile conditions.
[0028] In a further aspect, the present invention also relates to a process for the preparation of a composition according to the invention, comprising:
(a) preparing an aqueous solution comprising the phospholipid component and, if present, the stabilizer, salt and/or alcohol component;
(b) adjusting the pH of the solution of (a);
(c) adding to the aqueous solution of (b) the hyaluronan component;
(d) adjusting the pH of the solution of (c);
(e) homogenizing the solution by turbo-mixing; and
(f) sterilizing the solution of (e) by sterile filtration and/or moist heat.
Detailed Description
[0029] The following detailed description refers, by way of illustration, to specific details and embodiments in which the invention may be practiced.
[0030] The present invention generally relates to a formulation that is suitable as a replacement for the precorneal tear film and/or that can restore the precorneal tear film.
Other applications of the inventive compositions include the use as a carrier medium for the delivery of drugs, the use as a lubricant for ocular lenses, the use as a component of cosmetic formulations for eye or skin cosmetics. The compositions of the invention may also be suited for dermal applications, such as the treatment or prevention of dry skin or other conditions of the skin.
[0031] The compositions of the invention are aqueous compositions that comprise a phospholipid component and a hyaluronan component.
[0032] "Aqueous" as used herein means that the composition is water-based, i.e. that it includes water as a carrier or diluent.
[0033] In one embodiment of the invention, the composition comprises about 0.01 to about 5 % by weight, about 0.1 to about 3 % by weight, or about 1 % by weight of the phospholipid component.
[0034] As used herein, "about" relates to a variation of ±10 % of the value to which it refers. [0035] "% by weight", as used herein, relates to percent by weight of the composition unless indicated otherwise.
[0036] In one embodiment of the invention, the composition comprises about 0.01 to about 1 % by weight, about 0.05 to about 0.5 % by weight, or about 0.1 % by weight of the hyaluronan component.
[0037] In the compositions of the invention, the ratio of the phospholipid component and the hyaluronan component may be in the range of about 50: 1 to about 1:1, about 20: 1 to about 5:1, or about 10:1 by weight.
[0038] In preferred embodiments, the composition further comprises a stabilizing component. The stabilizing component increases the stability of the phospholipids and prevents the formulation from becoming rancid. The composition may comprise about 0.01 to about 1 % by weight, about 0.1 to about 0.5 % by weight, or about 0.16 % by weight of the stabilizing component.
[0039] The terms "stabilizing component" and "stabilizer" are used interchangeably herein and relate to compounds or compositions that increase the stability of the formulation, Le. prevent degradation of the phospholipid and/or hyaluronan component. In preferred embodiments, the stabilizer prevents degradation of the lipids and thus prevents the composition from becoming rancid. The use of a stabilizer may prolong the shelf-life of the composition and may also allow storage at room temperature without compromising the structural integrity of the contained lipids.
[0040] In one embodiment, the stabilizing component is selected from the group of urea and carbohydrates. In another embodiment, the carbohydrates are monosaccharides or derivatives thereof, such as sugar alcohols or amino sugars. The carbohydrates may, for example, be selected from the group consisting of glucose, fructose, mannose, galactose, sorbitol, mannitol and inositol.
[0041] In the compositions of the invention, the phospholipid component may comprise, consist essentially of, or consist of (i) phosphoglycerides, such as phosphatidic acids, cytidylic phosphoglycerides (CDP diglycerides), phosphatidylcholines, phosphatidylethanolamines, phosphatidylserine, phosphatidyl-N- methylethanolamine, phosphatidyl-N,N-dimethylethanolamine, phosphatidyl-N- acylethanolamine, phosphatidyI-N-2-hydroxyethylalanine, phosphatidylglycerol, phosphatidylglycerophosphate, diphosphatidylglycerol, lysobisphosphatidic acids, phosphatidylglucosaminylglycerol, phosphatidylinositol, phosphatidylinositol monophosphate and diphosphate, phosphatidylglucose, and phosphatidyl-O- diglucosylg!ycerol, and (ii) phosphosphingolipids, such as sphingomyelin (ceramide phosphorylcholine), ceramide phosphorylethanolamine, ceramide phosphorylglycerophosphate, and ceramide phosphorylinositol.
[0042] In a preferred embodiment, the phospholipid component comprises, consists essentially of, or consists of phosphatidylcholine (PC). The phosphatidylcholine may be fully saturated and may for example comprise or consist of dipalmitoyl- phosphatidylcholine (DPPC), distearoyl-phosphatidylcholine (DSPC) or mixtures thereof. Full saturation of the fatty acid residues may be achieved by appropriate chemical treatment of the PC, for example hydration.
[0043] In certain embodiments, the phosphatidylcholine is of natural or synthetic origin.
[0044] In the compositions of the invention, the hyaluronan component may be selected from hyaluronic acid, hyaluronic acids esters and hyaluronic acid salts or combinations thereof. The hyaluronic acid salts may be selected from the group consisting of sodium hyaluronate, potassium hyaluronate, iron hyaluronate, calcium hyaluronate, magnesium hyaluronate and zinc hyaluronan or combinations thereof.
[0045] In one embodiment, the molecules of the hyaluronan component have a mean molecular weight of about 100,000 to about 2,500,000 Da, about 100,000 to about
1,500,000 Da, about 500,000 to about 1,000,000 Da, or about 100,000 or about
1,000,000 Da.
[0046] In some embodiments, the composition of of the invention is hypoosmolar relative to a bodily fluid or body fluid compartment.
[0047] "Bodily fluid" as used herein relates to fluids natural occuring in the human or animal body and include, but are not limited to, blood, plasma, serum, lymph, cerebrospinal fluid, saliva, tear fluid, urine, sweat, and vaginal secretion. "Body fluid compartment" as used herein relates to a fluid compartment of the body and includes intracellular fluid and extracellular fluid, with the latter being subdivided into the extravascular compartment comprising interstitial fluid and the intravascular compartment comprising mainly blood. Usually these compartments are in osmotic equilibrium. The normal osmolarity of body fluids lies in the range of 280-320 mOsmol/1 H20. Isotonic saline (0.9% NaCl) has an osmolarity of 308 mOsmol/1.
[0048] In certain embodiments, the osmolarity of the composition is < 300 mOsmol/kg, < 250 mOsmol/kg, < 200 mOsmol/kg or about 150 mOsmol kg H20. The osmolarity may be controlled by a tonicity adjusting agent, such as the inorganic salts mentioned below, glycerol, sorbitol, mannitol, ethylene glycol, propylene glycol, and dextrose.
[0049] The compositions of the invention may be pharmaceutical or cosmetic compositions and may be for ophthalmic or dermal use.
[0050] In certain embodiments, the composition may fiirther comprise ions or salts. The salts may, for example, be inorganic salts. The ions may be, for example, alkaline metal or earth alkaline metal ions, including, but not limited to sodium, potassium, calcium and magnesium or combinations thereof. In some embodiment of the invention, the salts are selected from sodium chloride, potassium chloride, monosodium phosphate, disodium phosphate, sodium citrate, magnesium chloride, and calcium chloride or mixtures thereof. In specific embodiments, the ions or salts are essential for the homeostasis of cells on the corneal surface and comprise one or more ions selected from potassium, calcium and magnesium. These ions may be provided in form of salts. In one embodiment, one or more of these ions are provided in form of citrate salts. The use of citrate as an anion in these salts is preferred, because citrate acts as a chelating agent for calcium and magnesium ions and thus prevents the formation of insoluble salts of magnesium and calcium ions with the hyaluronan component. Alternatively, citrate ions may be provided separately, for example in form of sodium citrate.
[0051] In case the composition comprises potassium, magnesium and/or calcium ions, the concentrations of these ions are about 0.001 to about 0.1 % by weight of the formulation. In one specific embodiment, the potassium concentration is about 0.055 % by weight, and/or the magnesium concentration is about 0.0015 % by weight, and/or the calcium concentration is about 0.005 % by weight.
[0052] In certain embodiments of the invention, the composition may further comprise one or more auxiliaries, carriers or diluents.
[0053] The additional diluents may, for example, comprise propylene glycol. [0054] The auxiliaries may be selected from the group consisting of antioxidants, surfactants, buffering agents, preservatives, natural or synthetic oil components, alcohols, thickening agents, vitamins and inorganic salts or combinations thereof. The inorganic salts include, but are not limited to sodium chloride.
[0055] In one embodiment, the composition may comprise sodium chloride, for example in a concentration of about 0.01 to about 2 % by weight or about 0.1 to about 1 % by weight of the composition.
[0056] In one embodiment, the composition may comprise an antioxidant, such as a tocopherol, tocotrienol, tocomonoenol or marine tocopherol (MDT). The antioxidant may thus be selected from the group consisting of alpha-, beta-, gamma-, delta- tocopherol, alpha-, beta-, gamma-, delta-tocotrienol, alpha-, beta-, gamma-, delta- tocomonoenol, alpha-, beta-, gamma-, and delta-MDT and mixtures thereof.
[0057] In some embodiments, the inventived compositions comprise a natural or synthetic oil component selected from the group consisting of wax esters, mineral oils, synthetic hydrocarbons, silicon oils, natural and synthetic di- and triglycerides, and fatty acids.
[0058] In some embodiments, the composition comprises one or more alcohols selected from the group consisting of ethanol, propanol, isopropanol, 1,2- propyleneglycol, 1,3-butyleneglycol, glycerol or mixtures thereof.
[0059] Preservatives useful for the compositions of the invention are those that are non-irritating to the skin or the eye and which are compatible with the components of the composition. Suitable preservatives include but are not limited to sorbic acid and ethylenediamine tetraacetic acid (EDTA). The preservative may be used in a biostatic amount that prevents contamination of the composition. The preservative may include polyhexanide.
[0060] If the composition includes surfactants, these are preferyl non-ionic. Suitable examples include polysorbate, block copolymers of ethylene oxide and propylene oxide (Pluronic®), and polyethoxylated castor oil (Cremophor®).
[0061] Buffering agents that may be used in the invented compositions include, but are not limited to Tris (Tris(hydroxymethyl)aminomethane), NaOH, histidine, tricine, lysine, glycine and serine adjusted to the correct pH with an acidic component with low ionic force. [0062] The compositions may also comprise further auxiliaries such as hypromellose (hydroxypropylmethylcellulose), chitosan, lubricin, xanthan gum, parabens, benzalkonium chloride, polyhexamethylene biguanide, arginine, glycine, heparin, sodium pentosan polysuifate and/or lysine.
[0063] The invented compositions may have a pH in the range of from about 6 to about 8, about 7 or about 7.3.
[0064] In some embodiments, the compositions of the invention further comprise a pharmaceutically active component. The pharmaceutically active component may be selected from the group consisting of antibiotics, antifungals, analgesics, anesthetics, anti-allergics, and anti- inflammatory agents. The pharmaceutical may also be useful for the treatment of conditions of the eye, such as glaucoma, conjunctivitis, or other inflammatory conditions.
[0065] In a specific embodiment, the composition of the invention comprises, consists essentially of or consists of: 0.1 % by weight sodium hyaluronate, 1 % by weight phosphatidylcholine and 0.16 % by weight urea. The composition may further comprise: about 0.28 % by weight sodium chloride, about 0.1 % by weight potassium chloride, about 0.32 % by weight disodium phosphate dodecahydrate, about 0.026 % by weight sodium citrate, about 0.01 % by weight magnesium chloride hexahydrate, about 0.01 % by weight calcium chloride dehydrate, and about 5 % by weight glycerol. The composition may further comprise water to add up to 100 %. The afore-mentioned composition may have a pH of about 7.3.
[0066] The invented compositions can be in any form that allows application or administration, including, but not limited to a spray, dispersion, cream, gel or solution. The composition may be adapted for topical application.
[0067] In some embodiments, the composition is in single unit dose or multiple unit dose form.
[0068] In certain embodiments of the invention, the compositions are in a ready to use format. In other embodiments, they can form part of a kit comprising a concentrated form of the composition that has to be diluted with a suitable diluent before use. The diluent may, for example, be water. The diluent may also be contained in the kit. In such a kit, the components of the composition can be contained in separate containers and be combined before use. [0069] The compositions of the invention may be used for treating, preventing or alleviating a condition of the eye or the skin in a subject or patient in need thereof. This use may involve the manufacture of a pharmaceutical for such use. The condition of the eye treated, prevented or alleviated may be ocular dryness, dry eye syndrome, ocular discomfort, or Sjogren syndrome. The subject may be a mammal, preferably a human being.
[0070] The compositions of the invention may also be used for inhibiting the evaporation of the aqueous layer of the tear film.
[0071] Furthermore, another use of the compositions is as vehicles for ophthalmic or dermal medications. In such a use, the compositions serve as a carrier solution in which the drug to be delivered is solved, disperged, or emulgated.
[0072] In another aspect, the compositions of the invention may be used for the manufacture of a cosmetic product.
[0073] A further use of the invented compositions is as a lubricant for ocular lenses, such as contact lenses.
[0074] The invention also encompasses a method for the treatment, prevention or alleviation of a condition of the eye or the skin, wherein this method comprises administering a composition of the invention to a subject in need thereof. The condition of the eye may be ocular dryness, dry eye syndrome, ocular discomfort, or Sjogren syndrome. The subject may be a mammal, preferably a human being.
[0075] The compositions of the invention may be prepared according to a process comprising:
(a) sterilizing the phospolipid component by treatment with gamma-radiation or by moist heat;
(b) preparing an aqueous solution comprising the hyaluronan component and, if present, the stabilizer, salt and/or alcohol component;
(c) adjusting the pH of the solution of (b);
(d) sterilizing the solution of (c) by sterile filtration; and
(e) combining the phospholipids of (a) and the aqueous solution (d) by homogenization under sterile conditions.
[0076] Alternatively, the composition may be prepared by a process comprising: (a) preparing an aqueous solution comprising the phospholipid component and, if present, the stabilizer, salt and/or alcohol component;
(b) adjusting the pH of the solution of (a);
(c) adding to the aqueous solution of (b) the hyaluronan component;
(d) adjusting the pH of the solution of (c);
(e) homogenizing the solution by turbo-mixing; and
(f) sterilizing the solution of (e) by sterile filtration.
[0077] In both processes, preparation of the aqueous solution may be carried out at elevated temperature, for example at about 50 to about 90 °C.
[0078] The compositions of the present invention impart excellent lubrication at low load due to their content of hyaluronan. At points of high load they provide excellent boundary lubrication due to formation of an oligolamellar layer of phospholipid. The present compositions also have anti-adhesive properties due to their phospholipid content. They enhance the retention of fluid by the tear film by retaining water molecules that are associated with the long hydrophilic hyaluronic acid molecules and by providing a phospholipid monolayer reducing evaporation at the air-aqueous interface. The composition of the present invention also reduces film rupture by reducing the surface energies of both the tissue-liquid and air-liquid interfaces. The latter can be reduced by hyaluronic acid acting as a wetting acid, while the former can be reduced by locating a phospholipid monolayer on the surface of the tear film to reduce surface tension.
[0079] While the invention has been particularly shown and described with reference to specific embodiments, it should be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. The scope of the invention is thus indicated by the appended claims and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced. Examples
Example 1
[0080] The following formulation (Table 1) was prepared and tested:
Table 1
Figure imgf000014_0001
[0081] For the preparation, Solution 1 and Solution 2 (Table 2) were prepared separately and then combined.
Table 2
Figure imgf000014_0002
[0082] Solution I was prepared according to the following process:
A cleaned, stainless steel mixing tank (size: 100L) equipped with a stirrer was washed with steam (123°C to 138°C) for 30 minutes. The mixing tank number and the time were recorded. About 80% of the total amount of water for injections was set in the mixing tank. Temperature of the water for injections: 25°C ± 5°C. Then, the following substances were added one by one while stirring: sodium chloride, urea, glycerol and phosphatidycholine. These substances must be dissolved before any more of the substances are added. Stirring time was at least 15 minutes at 350 ± 50 rpm. If necessary, the pH value was adjusted to 7.2-7.4 with hydrochloric acid 10% or sodium hydroxide 4% solution. Stirring time was at least 15 minutes after each adding at 350 ± 50 rpm. The amount of hydrochloric acid 10% or sodium hydroxide 4% solution used was recorded. Hereafter the solution was brought to final weight (15.0 kg) with water for injections while stirring. Stir the solution for at least 10 minutes at 350 ± 50 rpm until a homogenous mixture was obtained. The emulsion resulting from this process was transferred into a cleaned, stainless steel autoclave (size: 15 L) equipped with a stirrer and the emulsion was exposed to 121°C for 15 minutes under slow agitation (350 ± 50 rpm). Samples were taken for quality control laboratory and sterility tests. After release by the Quality Control laboratory, the solution was kept under sterile filtered air (overpressure 0.5 - 1 bar) for further processing.
[0083] Solution 2 was prepared according to the following process:
A cleaned, stainless steel mixing tank (size: 100L) equipped with a stirrer was washed with steam (123°C to 138°C) for 30 minutes. The mixing tank number and the time were recorded. About 80% of the total amount of water for injections was set in the mixing tank. Temperature of the water for injections: 25°C + 5°C. Then, the following substances were added one by one while stirring: sodium citrate, sodium chloride, potassium chloride and sodium phosphate dodecahydrate. These substances must be dissolved before any more of the substances are added. Stirring time was at least 15 minutes at 350 ± 50 rpm. If necessary, the pH value was adjusted to 7.2-7.4 with hydrochloric acid 10% or sodium hydroxide 4% solution. Stirring time was at least 15 minutes after each adding at 350 ± 50 rpm. The amount of hydrochloric acid 10% or sodium hydroxide 4% solution used was recorded. Under stirring, the following substances were then added and dissolved: calcium chloride dihydrate and magnesium chloride hexahydrate. Stirring time was at least 15 minutes at 350 ± 50 rpm. Under slow stirring at 350 ± 50 rpm sodium hyaluronate was added and the stirring continued for at least 12 hours until complete dissolution. Care was taken to avoid lump formation. The pH (7.2-7.4) was checked, and, if necessary, corrected with hydrochloric acid 10% or 4% sodium hydroxide solution. Stirring time was at least 5 minutes after each adding at 350 ± 50 rpm. The amount of hydrochloric acid 1 % or sodium hydroxide 4% solution used was again recorded. Hereafter the solution was brought to final weight (85.0 kg) with water for injections while stirring. The solution was stirred for at least 10 minutes at 350 =fc 50 rpm until a homogenous mixture was obtained.. . Samples were taken for quality control laboratory tests and microbiological test. After release by the Quality Control laboratory, the solution was sterilised by sterile filtration with a filter having 0.22 μηι pore size into another sterilised mixing tank and kept under sterile filtered air (overpressure 0.5 - 1 bar) for further processing. The output after filtration was determined (97.0 to 100.5 %) and a filter integrity test carried out.
[0084] Solution 1 (sterile by moist heat) was step by step unified with solution 2 (sterile by filtration) in the mixing tank already containing solution 2. Stirring time was at least 15 minutes at 350 ± 50 rpm after having added the full quantity of solution 1. Finally, the resulting emulsion was aseptically filled into adequate monodose or multidose containers.
[0085] Table 3 shows a formulation with lower concentrations of sodium hyaluronate and phosphatidylcholine, which in addition contains lubricin
Table 3
Figure imgf000016_0001
[0086] Table 4 shows a formulation with lower concentrations of Sodium hyaluronate and phosphatidylcholine, which in addition contains chitosan
Table 4
Figure imgf000016_0002
Sodium citrate 26.0 mg
Magnesium chloride hexahydrate 9.2 mg
Calcium chloride dihydrate 8.9 mg
Glycerol 417.0 mg
Hydrochloric acid 10% (m/m) or
Sodium hydroxide solution 4% (m/m) q. s. ad pH 7.3
Water for injections ad 100.0 g
[0087] Table 5 shows a formulation which is sterile by the addition of Cosmocil (Polyhexanide) Table 5
Figure imgf000017_0001

Claims

Claims
What is claimed is: 1. Aqueous composition comprising a phospholipid component and a hyaluronan component.
2. The composition of claim 1, wherein the composition comprises about 0.01 to about 5 % by weight of the phospholipid component.
3. The composition of claim 2, wherein the composition comprises about 1% by weight of the phospholipid component
4. The composition of any of the preceding claims, wherein the composition comprises about 0.01 to about 1 % by weight of the hyaluronan component.
5. The composition of claim 4, wherein the composition comprises about 0.1 % by weight of the hyaluronan component.
6. The composition of any of the preceding claims, wherein the ratio of the phospholipid component and the hyaluronan component is in the range of about 50: 1 to about 1:1, about 20: 1 to about 5:1 or about 10:1 by weight
7. The composition of any of the preceding claims further comprising a stabilizing component.
8. The composition of claim 7, wherein the composition comprises about 0.01 to about 1 % by weight of the stabilizing component.
9. The composition of claim 8, wherein the composition comprises about 0.16
% by weight of the stabilizing component.
10. The composition of any one of claims 7-9, wherein the stabilizing component is selected from urea or monosaccharides.
1 1. The composition of claim 10, wherein the monosaccharides are selected from the group consisting of glucose, fructose, mannose, galactose, sorbitol and inositol.
12. The composition of any of the preceding claims, wherein the phospholipid component comprises, consists essentially of, or consists of phosphatidylcholine.
13. The composition of claim 12, wherein the phosphatidylcholine is fully saturated.
14. The composition of claim 12 or 13, wherein the phosphatidylcholine is of natural or synthetic origin.
15. The composition of any one of claims 12-14, wherein the phosphatidylchohn comprises dipalmitoyl-phosphatidylcholine (DPPC) or distearoyl- phosphatidy icholine (DSPC).
16. The composition of any of the preceding claims, wherein the hyaluronan component is selected from hyaluronic acid, hyaluronic acids esters and hyaluronic acid salts or combinations thereof.
17. The composition of claim 16, wherein the hyaluronic acid salts are selected from the group consisting of sodium hyaluronate, potassium hyaluronate, iron hyaluronate, calcium hyaluronate, magnesium hyaluronate and zinc hyaluronan or combinations thereof.
18. The composition of any of the preceding claims, wherein the molecules of the hyaluronan component have a mean molecular weight of about 100,000 to 2,500,000 Da.
19. The composition of claim 18, wherein the molecules of the hyaluronan component have a mean molecular weight of about 1,000,000 Da.
20. The composition of claim 18, wherein the molecules of the hyaluronan component have a mean molecular weight of about 100,000 Da.
21. The composition of any of the preceding claims, wherein the composition is hypoosmolar relative to a bodily fluid or body fluid compartment.
22. The composition of claim 21, wherein the osmolarity of the composition is < 300 mOsmol/kg, < 250 mOsmol/kg, < 200 mOsmoI kg, or about 150 mOsmol/kg ¾0.
23. The composition of any of the preceding claims, wherein the composition is a pharmaceutical or cosmetic composition.
24. The composition of claim 23, wherein the composition is for ophthalmic or dermal use.
25. The composition of any of the preceding claims, wherein the composition further comprises one or more auxiliaries, carriers or diluents.
26. The composition of claim 25, wherein the auxiliaries are selected of the group consisting of antioxidants, surfactants, preservatives, natural or synthetic oil components, alcohols, thickening agents, vitamins and inorganic salts or combinations thereof.
27. The composition of claim 26, wherein the antioxidant is a tocopherol.
28. The composition of claim 26, wherein the natural or synthetic oil components are selected from the group consisting of wax esters, mineral oils, a synthetic hydrocarbon, silicon oil, natural and synthetic di- and triglycerides, and fatty acids.
29. The composition of claim 26, wherein the alcohols are selected from the group consisting of ethanol, propanol, isopropanol, 1,2-propyleneglycoi, 1,3- butyleneglycol, glycerol or mixtures thereof.
30. The composition of any of the preceding claims, further comprise one or more compounds selected from the group consisting of hypromellose (hydroxypropylmethylcellulose), chitoson, lubricin, xanthan gum, parabcns, benzalkonium chloride, polyhexamethylene biguanide, arginine, glycine, lysine, heparin and sodium pentosan polysulfate.
31. The composition of any one of the preceding claims, wherein the composition further comprises ions or salts.
32. The composition of claim 31 , wherein the salts comprise inorganic salts.
33. The composition of claim 31, wherein the ions comprise alkaline metal or earth alkaline metal ions or both.
34. The composition of claim 31, wherein the salts are selected from sodium chloride, potassium chloride, monosodium phosphate, disodium phosphate, sodium citrate, magnesium chloride, and calcium chloride or mixtures thereof.
35. The composition of claim 33, wherein the ions are selected from the group consisting of potassium, sodium, calcium or magnesium ions or combinations thereof.
36. The composition of any of the preceding claims, wherein the pH of the composition is in the range of from about 6 to about 8.
37. The composition of claim 36, wherein the pH of the composition is about
7.3.
38. The composition of any of the preceding claims, wherein the composition further comprises a pharmaceutically active component.
39. The composition of claim 38, wherein the pharmaceutically active component is selected from the group consisting of antibiotics, antifungals, analgesics, anesthetics, anti-allergics, and anti-inflammatory agents.
40. The composition of any of the preceding claims, wherein the composition comprises: 0.1 % by weight sodium hyaluronate, 1 % by weight phosphatidylcholine and 0, 16 % by weight urea.
41. The composition of claim 40, further comprising: about 0.28 % by weight sodium chloride, about 0.1 % by weight potassium chloride, about 0.32 % by weight disodium phosphate dodecahydrate, about 0.026 % by weight sodium citrate, about 0.01 % by weight magnesium chloride hexahydrate, about 0.01 % by weight calcium chloride dihydrate, and about 5 % by weight glycerol.
42. The composition of claim 40 or 41, wherein the composition has a pH of about 7.3.
43. The composition of any of the preceding claims, wherein the composition is in form of a spray, dispersion, cream, gel or solution.
44. The composition of any of the preceding claims, wherein the composition is in single unit dose or multiple unit dose.
45. Use of the composition of any one of claims 1-44 for the manufacture of a medicinal product or medical device for treating, preventing or alleviating a condition of the eye or the skin in a patient in need thereof.
46. The use of claim 45, wherein the condition of the eye is ocular dryness, dry eye syndrome, ocular discomfort, or Sjogren syndrome.
47. Use of the composition of any one of claims 1-44 for the manufacture of a medicinal product or medical device for inhibiting the evaporation of the aqueous layer of the tear film.
48. Use of the composition of any one of claims 1-44 as vehicles for ophthalmic or dermal medications.
49. Use of the composition of any one of claims 1-44 for the manufacture of a cosmetic product.
50. Method for the treatment, prevention or alleviation of a condition of the eye or the skin, comprising administering a composition of any of claims 1-44 to a subject in need thereof.
51. The method of claim 50, wherein the condition of the eye is ocular dryness dry eye syndrome, ocular discomfort, or Sjogren syndrome.
52. The use of claim 45 or the method of claim 50, wherein the subject is a human.
53. Process for the preparation of a composition according to any one of claims
1-44, comprising:
(a) sterilizing the phospolipid component by treatment with gamma-radiation or by moist heat
(b) preparing an aqueous solution comprising the hyaluronan component and, if present, the stabilizer, salt and/or alcohol component
(c) adjusting the pH of the solution of (b);
(d) sterilizing the solution of (c) by sterile filtration; and (e) combining the phospholipids of (a) and the aqueous solution (d) by homogenization under sterile conditions.
54. Process for the preparation of a composition according to any one of claims
1-44, comprising:
(a) preparing an aqueous solution comprising the phospholipid component and, if present, the stabilizer, salt and/or alcohol component;
(b) adjusting the pH of the solution of (a);
(c) adding to the aqueous solution of (b) the hyaluronan component;
(d) adjusting the pH of the solution of (c);
(e) homogenizing the solution by turbo-mixing; and
(f) sterilizing the solution of (e) by sterile filtration and/or moist heat.
PCT/EP2011/056753 2010-05-04 2011-04-28 Aqueous composition for ophthalmic or dermal use WO2011138228A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US33094010P 2010-05-04 2010-05-04
US61/330,940 2010-05-04

Publications (1)

Publication Number Publication Date
WO2011138228A1 true WO2011138228A1 (en) 2011-11-10

Family

ID=44209940

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2011/056753 WO2011138228A1 (en) 2010-05-04 2011-04-28 Aqueous composition for ophthalmic or dermal use

Country Status (1)

Country Link
WO (1) WO2011138228A1 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019125166A3 (en) * 2017-12-22 2019-08-08 Ferring B.V. Hyaluronic acid formulations
WO2021045606A1 (en) * 2019-09-06 2021-03-11 Sophia Holdings, S.A. De C.V. Ophthalmic pharmaceutical composition, preparation methods and uses of same
EP3986914A4 (en) * 2019-06-19 2023-07-19 TearSolutions, Inc. Constrained peptides

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989001777A1 (en) * 1987-08-25 1989-03-09 Macnaught Pty. Limited Lubricant composition for rheumatism
WO1991012808A1 (en) * 1990-02-22 1991-09-05 Macnaught Pty Limited Artificial tears
DE4431251A1 (en) * 1994-09-02 1996-03-07 Audor Pharma Gmbh Skin cream, and base cream for cosmetics, eczema etc.
DE10033975A1 (en) * 2000-07-06 2002-01-24 Coty Bv Gelled solid cosmetic composition, in the form of a pseudoelastic gel with greater elasticity than viscosity, comprises water-soluble gelling agent and phospholipid
WO2009084069A2 (en) * 2008-01-02 2009-07-09 Lio Farmaceutici Srl Ocular solution with organic lycopene

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989001777A1 (en) * 1987-08-25 1989-03-09 Macnaught Pty. Limited Lubricant composition for rheumatism
WO1991012808A1 (en) * 1990-02-22 1991-09-05 Macnaught Pty Limited Artificial tears
DE4431251A1 (en) * 1994-09-02 1996-03-07 Audor Pharma Gmbh Skin cream, and base cream for cosmetics, eczema etc.
DE10033975A1 (en) * 2000-07-06 2002-01-24 Coty Bv Gelled solid cosmetic composition, in the form of a pseudoelastic gel with greater elasticity than viscosity, comprises water-soluble gelling agent and phospholipid
WO2009084069A2 (en) * 2008-01-02 2009-07-09 Lio Farmaceutici Srl Ocular solution with organic lycopene

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019125166A3 (en) * 2017-12-22 2019-08-08 Ferring B.V. Hyaluronic acid formulations
EP3986914A4 (en) * 2019-06-19 2023-07-19 TearSolutions, Inc. Constrained peptides
WO2021045606A1 (en) * 2019-09-06 2021-03-11 Sophia Holdings, S.A. De C.V. Ophthalmic pharmaceutical composition, preparation methods and uses of same

Similar Documents

Publication Publication Date Title
RU2495661C2 (en) Pharmaceutical formulations of non-polar and polar lipids for ophthalmological application
KR20130139925A (en) Liquid pharmaceutical composition for the delivery of active ingredients
IL182532A (en) Topical ophthalmic composition and an artificial tear for the treatment of dry eye and use of the composition or the artificial tear for the manufacture of a medicament
US9801899B2 (en) Artificial tear emulsion
EP2494954B1 (en) Ophthalmic composition based on lactobionic acid useful for reducing corneal edema and inflammation
EP2299974A1 (en) Pharmaceutical compositions containing a fluoroquinolone antibiotic drug
RU2302231C1 (en) Eye drops for treating dry eye syndrome
CN104814924A (en) Brinzolamide liposome eye preparation and preparation method thereof
WO2011138228A1 (en) Aqueous composition for ophthalmic or dermal use
EP1551417B1 (en) Three layer tear formulation
KR20150004798A (en) Vesicular Formulations
IT201800007677A1 (en) LIPOSOMES FOR THE TREATMENT OF OCULAR PATHOLOGIES
JP5920875B2 (en) Novel formulations for the treatment of fungal infections
WO2013046059A2 (en) Methods and compositions for tamarind-based ocular disease treatment in combination with trehalose
US20170239307A1 (en) Composition of doxycycline in liposomes for the prevention, improvement and/or treatment of ocular pathologies
US20190038555A1 (en) Bicontinuous microemulsions and methods of use thereof
US20190151352A1 (en) Ophthalmic composition comprising a synergistic combination of glycogen and hyaluronic acid or a salt thereof
KR20080077585A (en) Ophthalmic composition for preventing and treating ocular diseases
US20220143075A1 (en) Ophthalmic pharmaceutical compositions and methods for treating ocular surface disease
JP2023505409A (en) In-situ gel containing cyclosporine micelles as a sustained-acting ocular drug delivery system
TR201603016A1 (en) OPHTHALMIC PHARMACEUTICAL COMPOSITIONS CONTAINING POLYMER
CN103860467A (en) Bromfenac sodium lipidosome eye drop
Yeh Evolution of Systane® Artificial Tears
TR201603173A1 (en) OPHTHALMIC PHARMACEUTICAL COMPOSITIONS

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 11718695

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 11718695

Country of ref document: EP

Kind code of ref document: A1