WO2011133493A3 - Allosteric regulation of kinase activity - Google Patents

Allosteric regulation of kinase activity Download PDF

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Publication number
WO2011133493A3
WO2011133493A3 PCT/US2011/032958 US2011032958W WO2011133493A3 WO 2011133493 A3 WO2011133493 A3 WO 2011133493A3 US 2011032958 W US2011032958 W US 2011032958W WO 2011133493 A3 WO2011133493 A3 WO 2011133493A3
Authority
WO
WIPO (PCT)
Prior art keywords
fusion proteins
kinase
kinase activity
allosteric regulation
ligand
Prior art date
Application number
PCT/US2011/032958
Other languages
French (fr)
Other versions
WO2011133493A2 (en
Inventor
Klaus Hahn
Andrei Karginov
Original Assignee
The University Of North Carolina At Chapel Hill
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The University Of North Carolina At Chapel Hill filed Critical The University Of North Carolina At Chapel Hill
Publication of WO2011133493A2 publication Critical patent/WO2011133493A2/en
Publication of WO2011133493A3 publication Critical patent/WO2011133493A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)

Abstract

The present invention relates to fusion proteins comprising a kinase and a ligand binding domain inserted in the kinase in or near the catalytic domain to produce a ligand-dependent kinase. The invention further relates to polynucleotides and vectors encoding the fusion proteins, cells comprising the fusion proteins, and methods of using the fusion proteins to study kinase function and analyze cellular activity, as well as diagnostic and therapeutic methods.
PCT/US2011/032958 2010-04-19 2011-04-19 Allosteric regulation of kinase activity WO2011133493A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US32575210P 2010-04-19 2010-04-19
US61/325,752 2010-04-19

Publications (2)

Publication Number Publication Date
WO2011133493A2 WO2011133493A2 (en) 2011-10-27
WO2011133493A3 true WO2011133493A3 (en) 2012-03-01

Family

ID=44834752

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2011/032958 WO2011133493A2 (en) 2010-04-19 2011-04-19 Allosteric regulation of kinase activity

Country Status (1)

Country Link
WO (1) WO2011133493A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016197136A2 (en) 2015-06-04 2016-12-08 Nmc, Inc. Improved productivity and bioproduct formation in phototropin knock/out mutants in microalgae
CN116875518A (en) 2018-07-06 2023-10-13 科罗拉多州立大学董事会法人团体 Gene coding system for constructing and detecting bioactive agents

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6127521A (en) * 1994-05-27 2000-10-03 Ariad Pharmaceuticals, Inc. Immunosuppressant target proteins
US20030206891A1 (en) * 1995-06-07 2003-11-06 Ariad Gene Therapeutics, Inc. Rapamycin-based biological regulation
US20040038400A1 (en) * 2002-08-26 2004-02-26 Froehlich Allan C. Methods for regulating gene expression using light

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6127521A (en) * 1994-05-27 2000-10-03 Ariad Pharmaceuticals, Inc. Immunosuppressant target proteins
US20030206891A1 (en) * 1995-06-07 2003-11-06 Ariad Gene Therapeutics, Inc. Rapamycin-based biological regulation
US20040038400A1 (en) * 2002-08-26 2004-02-26 Froehlich Allan C. Methods for regulating gene expression using light

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ANDREI V. KARGINOV ET AL.: "Engineered allosteric activation of kinases in living cells", NATURE BIOTECHNOLOGY, vol. 28, no. 7, 27 June 2010 (2010-06-27), pages 743 - 747 *
ANDREI V. KARGINOV ET AL.: "Light Regulation of Protein Dimerization and Kinase Activity in Living Cells Using Photocaged Rapamycin and Engineered FKBP", J AM CHEM SOC., vol. 133, no. 3, 16 December 2010 (2010-12-16), pages 420 - 423 *

Also Published As

Publication number Publication date
WO2011133493A2 (en) 2011-10-27

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