WO2011130636A3 - Methods and compositions for identifying mutations in cancer related genes - Google Patents

Methods and compositions for identifying mutations in cancer related genes Download PDF

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Publication number
WO2011130636A3
WO2011130636A3 PCT/US2011/032695 US2011032695W WO2011130636A3 WO 2011130636 A3 WO2011130636 A3 WO 2011130636A3 US 2011032695 W US2011032695 W US 2011032695W WO 2011130636 A3 WO2011130636 A3 WO 2011130636A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
compositions
related genes
cancer related
identifying mutations
Prior art date
Application number
PCT/US2011/032695
Other languages
French (fr)
Other versions
WO2011130636A2 (en
Inventor
Lynne Bemis
William A. Robinson
Original Assignee
The Regents Of The University Of Colorado, A Body Corporate
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The Regents Of The University Of Colorado, A Body Corporate filed Critical The Regents Of The University Of Colorado, A Body Corporate
Publication of WO2011130636A2 publication Critical patent/WO2011130636A2/en
Publication of WO2011130636A3 publication Critical patent/WO2011130636A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • Oncology (AREA)
  • Hospice & Palliative Care (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Provided herein are kits and PCR-based methods for detecting genetic variant sequences. The invention provides the rapid and sensitive CutBack Technique for detecting genetic variants that can determine an individual's reaction to therapy or prognosis.
PCT/US2011/032695 2010-04-16 2011-04-15 Methods and compositions for identifying mutations in cancer related genes WO2011130636A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US32524310P 2010-04-16 2010-04-16
US61/325,243 2010-04-16

Publications (2)

Publication Number Publication Date
WO2011130636A2 WO2011130636A2 (en) 2011-10-20
WO2011130636A3 true WO2011130636A3 (en) 2012-03-08

Family

ID=44799342

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2011/032695 WO2011130636A2 (en) 2010-04-16 2011-04-15 Methods and compositions for identifying mutations in cancer related genes

Country Status (1)

Country Link
WO (1) WO2011130636A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111587292A (en) * 2018-01-07 2020-08-25 纽克莱克斯有限公司 Kits and methods for detecting cancer-associated mutations
WO2024003936A1 (en) * 2022-06-29 2024-01-04 Raman Govindarajan Method for detecting cancer susceptibility, early detection and predicting cancer behaviour

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ASANO, H. ET AL.: "Detection of EGFR gene mutation in lung cancer by mutant-enriched polymerase chain reaction assay.", CLINICAL CANCER RESEARCH., vol. 12, no. 1, 1 January 2006 (2006-01-01), pages 43 - 48 *
FISCHER, C. ET AL.: "Enrichment of mutant KRAS alleles in pancreatic juice by subtractive iterative polymerase chain reaction.", LABORATORY INVESTIGATION., vol. 81, no. 6, June 2001 (2001-06-01), pages 827 - 831 *
KE, X. ET AL. ET AL.: "PCR designer for restriction analysis of various types of sequence mutation.", BIOINFORMATICS., vol. 18, no. 12, December 2002 (2002-12-01), pages 1688 - 1689 *
LIU, W. H. ET AL.: "Detection of hotspot mutations and polymorphisms using an enhanced PCR-RFLP approach.", HUMAN MUTATION., vol. 21, no. 5, May 2003 (2003-05-01), pages 535 - 541 *
ROHLFS, E. M. ET AL.: "Direct detection of mutations in the breast and ovarian cancer susceptibility gene BRCA1 by PCR-mediated site-directed mutagenesis.", CLINICAL CHEMISTRY, vol. 43, no. 1, January 1997 (1997-01-01), pages 24 - 29 *

Also Published As

Publication number Publication date
WO2011130636A2 (en) 2011-10-20

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