WO2011107299A3 - Method for the manufacture of recombinant dspa alpha1 - Google Patents

Method for the manufacture of recombinant dspa alpha1 Download PDF

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Publication number
WO2011107299A3
WO2011107299A3 PCT/EP2011/001884 EP2011001884W WO2011107299A3 WO 2011107299 A3 WO2011107299 A3 WO 2011107299A3 EP 2011001884 W EP2011001884 W EP 2011001884W WO 2011107299 A3 WO2011107299 A3 WO 2011107299A3
Authority
WO
WIPO (PCT)
Prior art keywords
alpha1
manufacture
rdspa
cation exchange
dspa
Prior art date
Application number
PCT/EP2011/001884
Other languages
French (fr)
Other versions
WO2011107299A2 (en
WO2011107299A9 (en
Inventor
Oliver Kops
Damian Leschik
Achim SCHÜTTLER
Sabine Sembries
Erno Pungor
Michael Mccaman
Thilo Grob
Frank Kohne
Jeroen Meijer
Jos Niessen
Original Assignee
Paion Deutschland Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Paion Deutschland Gmbh filed Critical Paion Deutschland Gmbh
Priority to EP11719763A priority Critical patent/EP2558580A2/en
Publication of WO2011107299A2 publication Critical patent/WO2011107299A2/en
Publication of WO2011107299A3 publication Critical patent/WO2011107299A3/en
Publication of WO2011107299A9 publication Critical patent/WO2011107299A9/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21068—Tissue plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A method for the manufacture of rDSPA alpha1 on a commercial scale is disclosed which comprises various downstream process steps inter alia a cation exchange chromatography for capturing rDSPA alpha1 from the conditioned culture medium. The step of washing the cation exchange resin comprises the change of flow direction. The invention further pertains to rDSPA alpha1 obtainable by this method.
PCT/EP2011/001884 2010-04-16 2011-04-14 Method for the manufacture of recombinant dspa alpha1 WO2011107299A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP11719763A EP2558580A2 (en) 2010-04-16 2011-04-14 Method for the manufacture of recombinant dspa alpha1

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US32491710P 2010-04-16 2010-04-16
EP10160221.7 2010-04-16
EP10160221 2010-04-16
US61/324,917 2010-04-16

Publications (3)

Publication Number Publication Date
WO2011107299A2 WO2011107299A2 (en) 2011-09-09
WO2011107299A3 true WO2011107299A3 (en) 2011-12-01
WO2011107299A9 WO2011107299A9 (en) 2012-04-05

Family

ID=44542649

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2011/001884 WO2011107299A2 (en) 2010-04-16 2011-04-14 Method for the manufacture of recombinant dspa alpha1

Country Status (2)

Country Link
EP (1) EP2558580A2 (en)
WO (1) WO2011107299A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3302784B1 (en) 2015-06-05 2021-10-06 W.R. Grace & Co.-Conn. Adsorbent bioprocessing clarification agents and methods of making and using the same
US10848363B2 (en) 2017-11-09 2020-11-24 Qualcomm Incorporated Frequency division multiplexing for mixed numerology
CN113929733A (en) * 2021-10-25 2022-01-14 江苏帆博生物制品有限公司 Monoclonal antibody purification method of mixed ion exchange filler
CN114150007B (en) * 2021-12-14 2023-07-21 扬州大学 Coding gene applicable to rabbit mammary gland specific expression deaminase and application thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997029188A1 (en) * 1996-02-05 1997-08-14 Schering Aktiengesellschaft METHOD FOR THE PRODUCTION OF rDSPA α1

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU642404B2 (en) 1989-02-13 1993-10-21 Schering Aktiengesellschaft Berlin Und Bergkamen Novel thrombolytic
DE10153601A1 (en) 2001-11-02 2003-05-22 Paion Gmbh DSPA for the treatment of stroke
JP4500280B2 (en) 2005-06-08 2010-07-14 日立オートモティブシステムズ株式会社 Powder drug dispenser

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997029188A1 (en) * 1996-02-05 1997-08-14 Schering Aktiengesellschaft METHOD FOR THE PRODUCTION OF rDSPA α1

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
GRASLUND SUSANNE ET AL: "Protein production and purification", NATURE METHODS, NATURE PUBLISHING GROUP, GB, vol. 5, no. 2, 1 February 2008 (2008-02-01), pages 135 - 146, XP002488686, ISSN: 1548-7091, [retrieved on 20080130], DOI: 10.1038/NMETH.F.202 *
SCHIERMEYER ANDREAS ET AL: "Production of Desmodus rotundus salivary plasminogen activator alpha1 (DSPAalpha1) in tobacco is hampered by proteolysis.", BIOTECHNOLOGY AND BIOENGINEERING 30 MAR 2005 LNKD- PUBMED:15685597, vol. 89, no. 7, 30 March 2005 (2005-03-30), pages 848 - 858, XP002660093, ISSN: 0006-3592 *
WEI ET AL: "Optimized gene synthesis, expression and purification of active salivary plasminogen activator alpha2 (DSPAalpha2) of Desmodus rotundus in Pichia pastoris", PROTEIN EXPRESSION AND PURIFICATION, ACADEMIC PRESS, SAN DIEGO, CA, vol. 57, no. 1, 21 November 2007 (2007-11-21), pages 27 - 33, XP022355826, ISSN: 1046-5928, DOI: 10.1016/J.PEP.2007.09.001 *

Also Published As

Publication number Publication date
EP2558580A2 (en) 2013-02-20
WO2011107299A2 (en) 2011-09-09
WO2011107299A9 (en) 2012-04-05

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