WO2011099809A2 - Chambre de comptage pour particules fines en quantité fixe et appareil d'analyse d'images d'échantillon mettant en oeuvre cette chambre - Google Patents

Chambre de comptage pour particules fines en quantité fixe et appareil d'analyse d'images d'échantillon mettant en oeuvre cette chambre Download PDF

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Publication number
WO2011099809A2
WO2011099809A2 PCT/KR2011/000932 KR2011000932W WO2011099809A2 WO 2011099809 A2 WO2011099809 A2 WO 2011099809A2 KR 2011000932 W KR2011000932 W KR 2011000932W WO 2011099809 A2 WO2011099809 A2 WO 2011099809A2
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Prior art keywords
channel
plate
quantitative
counting chamber
channel cover
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PCT/KR2011/000932
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English (en)
Korean (ko)
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WO2011099809A3 (fr
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임현창
정연철
조근창
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(주)로고스바이오시스템스
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Publication of WO2011099809A2 publication Critical patent/WO2011099809A2/fr
Publication of WO2011099809A3 publication Critical patent/WO2011099809A3/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/025—Align devices or objects to ensure defined positions relative to each other
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1486—Counting the particles

Definitions

  • the present invention relates to a quantitative microparticle counting chamber and a sample image analysis apparatus using the same, and more particularly, a channel cover part forming a channel is configured to elastically close contact with a channel forming region of a second plate.
  • a quantitative microparticle counting chamber capable of easily counting particles or cells, and a sample image analysis apparatus using the same.
  • the hemocytometer a counting chamber commonly used for cell counting, is a device for defining a fixed volume.
  • a glass jaw is manufactured by using a glass jaw to define the height of the lower and upper plates. To maintain a precise height.
  • Traditional hemocytometers have a separate top plate (cover glass) that serves as a cover and a bottom plate that determines the height of the chamber for reuse.
  • counting chambers counting chamber-Sedgwick-Rafter, Palmer-Maloney, Hemocytometers, Petroff-Hausser, Makler, Utermohl, etc.
  • These counting chambers are of constant size and use only the corresponding cover glass, and most of them are designed for quantitative analysis.
  • FIG. 18 a counting chamber according to the prior art is shown in FIG. 18.
  • the counting chamber 10 is provided between the upper plate 11 and the upper plate 11 formed by keeping the injection portion 14 and the discharge portion 15 at a distance from each other.
  • the lower plate 12 is configured to form the channel 13 and is coupled to the bottom of the upper plate 11.
  • the counting chamber 10 having such a structure, when the sample is injected into the injection hole 14, the injected sample is filled in the channel by capillary action.
  • the counting chamber according to the prior art has the following problems.
  • the technical problem of the present invention is to solve the above-mentioned problems of the prior art, and it is possible to quickly and easily combine the first and second plates of the chamber, thereby significantly reducing the manufacturing process and manufacturing cost. To provide a means to do so.
  • Another technical problem of the present invention is to provide a means capable of preventing a leak of a solution by allowing the components forming the channels of the first and second plates to have a tensile force and to be in close contact with elastic force.
  • Another technical problem of the present invention is to make a channel formed between the first and second plates have a constant volume and a uniform height.
  • the first plate ; And a second plate coupled to the first plate to form a channel in which a solution for microparticle counting is formed, wherein the first plate comprises: a channel cover part; A first joint part spaced apart from the outer circumference of the channel cover part by a predetermined distance; And a tensile force generating connection part interconnecting the channel cover part and the first junction part such that the channel cover part elastically comes into close contact with the channel forming region of the second plate when the first plate and the second plate are coupled to each other. It is achieved by a quantitative microparticle counting chamber characterized in that.
  • the tensile force generating connecting portion may be a plurality of tensile force generating connecting portion provided spaced apart from each other between the channel cover portion and the first junction.
  • the tensile force generating connection portion may be provided to have a thickness thinner than the channel cover portion and the first bonding portion.
  • the tensile force generating connection portion may be provided in an upper region of a vertical surface facing the channel cover portion and the first junction portion.
  • the tensile force generating connection portion has a plurality of straight lines curved or interconnected with the channel cover portion and the first junction portion so as to increase the connection distance relatively compared to connecting the channel cover portion and the first junction portion at the shortest distance. Can be connected.
  • the second plate may include a second joining portion formed at an edge to be coupled to the first joining portion; A bottom portion recessed downward from the second bonding portion in an upper surface central region; A channel portion protruding from the bottom portion; And protruding to form a closed loop from the bottom portion at a predetermined distance from the channel portion, wherein the bottom edge region of the channel cover portion is in close contact with the channel portion to form a channel between the channel portion and the channel cover portion. It may include a support wall protruding to a large thickness.
  • the thickness of the support wall is greater than the thickness of the second joining part so that the bottom surface of the channel cover part is supported by the upper surface of the support wall when the first joining part and the second joining part are joined. Can be large.
  • the support wall may be formed in any one shape selected from a polygon, a circle, and an oval.
  • a reservoir portion may be formed between the support wall and the channel portion to primarily receive the solution.
  • the first junction part and the second junction part may be coupled by a coupling means.
  • the coupling means may include at least one hook projecting from a bottom surface of the first joining portion to be spaced apart from each other along a circumference of the first joining portion; And at least one hook insertion groove formed in the second joint portion at a position corresponding to the hook so that the hook can be inserted.
  • the hook has a body portion provided to have a circular cross-sectional shape; And at least one deformation rib protruding from a surface along a circumference of the body portion so as to be spaced apart from each other by a predetermined distance.
  • the coupling means may include: at least one post protruding from a bottom surface of the first junction portion to be spaced apart from each other along a circumference of the first junction portion; And at least one post insertion hole formed in the second joint part at a position corresponding to the post so that the post can be inserted therethrough.
  • a locking step may be formed to have elasticity and protrude outward from the surface.
  • the first junction part and the second junction part may be coupled to each other by a coupling means, and then the coupling part or the channel part may be sealed by solvent bonding or ultrasonic bonding.
  • the technical problem is a quantitative microparticle counting chamber in which a sample is accommodated; A light source unit irradiating light to the sample side accommodated in the quantitative microparticle counting chamber; An objective lens for enlarging an image of the sample formed by light emitted from the light source unit; An image acquisition unit for capturing an image of the sample enlarged through the objective lens; An image reading unit which reads an image of the sample photographed by the image obtaining unit; And a moving stage for moving the quantitative microparticle counting chamber such that a specific region of the quantitative microparticle counting chamber to be photographed is at an incident position of the light source unit, wherein the quantitative microparticle counting chamber comprises: a first plate; And a second plate coupled to the first plate to form a channel in which a solution for microparticle counting is formed, wherein the first plate comprises: a channel cover part; A first joint part spaced apart from the outer circumference of the channel cover part by a predetermined distance; And a tensile force generating connection part interconnecting the channel cover part and
  • the channel cover portion formed on the first plate is elastically in close contact with the support wall of the second plate by the tension generating connection portion, the channel cover portion and the support wall can keep the airtight, and thus the solution contained in the channel leaks. It is possible to provide a quantitative microparticle counting chamber capable of preventing the phenomenon and a sample image analyzing apparatus using the same.
  • the channel cover part is closely and closely adhered to the support wall, the contact surface is even, thereby providing a quantitative microparticle counting chamber having a uniform channel volume and a uniform channel height, and a sample image analyzing apparatus using the same. do.
  • FIG. 1 is a perspective view showing a chamber according to an embodiment of the present invention.
  • FIG. 2 is a bottom view illustrating the bottom of the first plate illustrated in FIG. 1.
  • 3 to 8 are bottom views showing the bottom of the first plate according to another embodiment of the present invention.
  • FIG. 9 is a perspective view of the coupled state of the chamber shown in FIG.
  • FIG. 10 10, 11, and 12 are perspective and partially enlarged cross-sectional views of the hook shown in FIG. 1, respectively.
  • 13, 14, and 15 are cross-sectional views taken along line A-A, line B-B, and line C-C of FIG. 9, respectively.
  • FIG. 16 is a partially enlarged cross-sectional view showing another embodiment of the coupling unit shown in FIG.
  • FIG. 17 is a schematic diagram of a sample image analysis apparatus using the chamber of FIG. 1.
  • FIG. 18 is a perspective view of a chamber according to the prior art.
  • the chamber although the term itself refers to the space in which the sample is applied, in the present invention "chamber" means a constant volume of space between the first and second plates of a transparent material widely used in the art. It refers to the product formed. That is, in the present invention, the "chamber” refers to a tool used to qualitatively and quantify an individual in a sample through an optical method after injecting a sample into an inner space of a channel formed by the combination of the first and second plates.
  • FIG. 1 is a perspective view showing a chamber according to a preferred embodiment of the present invention
  • Figure 2 is a bottom view showing the bottom of the first plate shown in Figure 1
  • Figures 3 to 8 is another embodiment of the present invention
  • FIG. 9 is a bottom view illustrating a bottom surface of a first plate according to an example
  • FIG. 9 is a perspective view illustrating a coupled state of the chamber illustrated in FIG. 1.
  • 10, 11, and 12 are perspective views and partially enlarged cross-sectional views showing the hooks shown in FIG. 1
  • FIGS. 13, 14, and 15 are cross-sectional views taken along lines A-A, B-B, and C-C of FIG. 9.
  • the quantitative microparticle counting chamber 100 (hereinafter, referred to as the “chamber 100”) according to an embodiment of the present invention has a channel cover in which an inlet 220 and an outlet 230 are formed.
  • the channel part 330 and the channel cover part 240 are provided by the first plate 200 having the part 240, the channel part 330, and the support wall 340 and the first plate 200.
  • the second plate 300 to form a channel between the ().
  • the first plate 200 and the second plate 300 are made of a transparent material.
  • the first plate 200 has an injection hole 220 formed to inject a sample (solution) and air in the channel 400 when the sample is injected into the injection hole 220.
  • connection part 260 interconnecting the first junction part 250 forming the edge of the first plate 200 and the channel cover part 240 forming the center is further provided.
  • the tensile force generating connection portion 260 is to generate a tension pulling the channel cover portion 240 in all directions so that the channel cover portion 240 is in close contact with the support wall 340 of the second plate 300, It is formed to a thickness thinner than the channel cover portion 240 and the first junction portion (250). This is because a small elastic force (or tensile force) is generated when the thickness of the tensile force generating connecting portion 260 is thick so as to have a sufficient elastic force by forming a thinner thickness than the channel cover portion 240 and the first junction portion (250).
  • each tensile force generating connection portion 260 is formed in the upper region of the vertical surface (240A, 250A) facing the channel cover portion 240 and the first bonding portion 250 as shown in FIG. This is because when the channel cover part 240 is in contact with the support wall 340, the first and second joint parts 250 and 310 are firmly coupled by the hook 270, which is a coupling means. Deformation to the lower side to generate an elastic force (tensile force) to ensure that the channel cover portion 240 is in close contact with the support wall 340 elastically.
  • both sides of the tension generating connector 260 are integrally formed in the upper regions of the vertical surfaces 240A and 250A, respectively.
  • the tensile force generating connection portion (260a. 260b, 260c, 260d, 260e, 260f) is a channel cover 240 and the first junction 250 It may also be provided as a plurality of straight lines that are curved or interconnected between).
  • the curved line and the straight line mean a member having a constant volume whose cross section is curved or straight.
  • the tension generating connection portions 260a, 260b, 260c, 260d, 260e, and 260f having various shapes may have the shortest distance from the channel cover 240 and the first junction 250 as shown in FIG. 2. It has a relatively increased connection distance compared to the linear force generating connection portion 260 of the linear connection.
  • each tensile force generating connection portion (260a. 260b, 260c, 260d, 260e, 260f) is deformed to generate elastic force (tensile force) so that the channel cover portion 240 elastically adheres to the support wall 340.
  • the inlet 220a and the outlet 230b are respectively different from the first plate 200 of the above-described embodiment. Only one is formed.
  • only one channel portion is also formed in the second plate 300 which will be described later. Accordingly, the first plate 200a, 200b, 200c, 200d, 200e, 200f and the second plate (not shown) When combined, only one channel (not shown) is formed.
  • the second plate 300 is coupled to the first plate 200 to form a channel 400 therebetween, and the second plate 300 is coupled to the first junction 250.
  • the second junction 310 formed at the edge in a shape corresponding to the first junction 250, the bottom 320 formed to be recessed to a certain depth in the upper surface center region, and protruded upward from the bottom 320.
  • the channel part 330 is formed, and the support wall 340 is formed to protrude in a figure shape closed from the bottom part 320 at a position spaced apart from the channel part 330 by a predetermined distance.
  • the support wall 340 is larger than the channel portion 330 such that the bottom edge region of the channel cover portion 240 is in close contact with each other so that the channel 400 is formed between the channel portion 330 and the bottom surface of the channel cover portion 240. Protruding to a thickness is formed.
  • the channel portion 330 is formed long in the longitudinal direction so that both sides thereof correspond to the inlet 220 and the outlet 230, respectively, but is not limited thereto.
  • the entire thickness thereof is not thickened more than necessary. This is because the channel part 330 is formed in the recessed bottom part 320 of the second plate 300.
  • the thickness of the support wall 340 is second such that the bottom surface of the channel cover 240 is supported by the top surface of the support wall 340. It is formed larger than the thickness of the junction 310.
  • the support wall 340 may be formed in any one shape selected from polygonal, circular, oval.
  • a rectangular shape is formed around the channel part 330 formed to extend in the longitudinal direction, and the cross section of the support wall 340 is formed in a quadrangular shape. This is to increase adhesion and airtightness with the channel cover portion 240, but is not limited thereto, and may be formed of a circle, an ellipse, a polygon, or the like.
  • a reservoir portion 350 is formed between the support wall 340 and the channel portion 330 at a position corresponding to the injection hole 220 provided in the first plate 220.
  • An inclined surface 360 tapered toward the upper end is formed at one side of the channel part 330 in contact with the part 350. The inclined surface 360 is to allow the solution contained in the reservoir 350 to easily move to the upper surface of the channel portion 330, that is, the channel 400.
  • coupling means for coupling the first and second plates 200 and 300 in a one-touch manner is provided at the first junction 250 and the second junction 310, respectively, as shown in FIGS. 10, 4b and 4c. That is, at least one hook 270 is formed at the bottom of the first junction 250, and the hook insertion grooves 370 are formed at positions corresponding to the hooks 270 at the second junction 310, respectively. Since the hook 270 is fitted into the hook insertion groove 370, the first and second plates 200 and 300 are coupled in a one-touch fitting manner.
  • the hook 270 is formed to protrude from the bottom of the first bonding portion 250, as shown in Figure 10 and 11, of the body portion 270A and the body portion 270A provided to have a circular cross-sectional shape At least one deformation rib 270B protruding from the surface along the circumference.
  • the hook insertion groove 370 is recessed in the second junction 310. The deformed rib 270B is deformed when the body portion 270A is fitted into the hook insertion groove 370, and is sandwiched between the body portion 270A and the hook insertion groove 370, thereby inserting the body portion 270A into the hook insertion groove. It has a function to fix firmly to 370.
  • the chamber 100 may include at least one channel by forming a plurality of channel cover parts 240 and channel parts 330.
  • the first junction 250 and the second junction 310 are in close contact with each other, and the hook 270 provided as a coupling means hooks 270. Insert into the insertion groove (370). That is, the first bonding portion 250 and the second bonding portion 310 are pressed from the outside so that the hook 270 of the first bonding portion 250 is fitted into the hook insertion groove 370 of the second bonding portion 310.
  • the first junction 250 and the second junction 310 are firmly, quickly and easily coupled.
  • the bottom edge of the channel cover 240 is in close contact with the upper surface of the support wall 340.
  • the tensile force generating connecting portion 260 formed thinner than the channel cover portion 240 and the first junction portion 250 is connected to the upper regions of the vertical surfaces 240A and 250A, respectively, so that the channel cover portion 240 and the first junction portion 250 are formed.
  • the tension generating connector 260 generates an elastic force by pulling the channel cover part 240 in all directions, and at the same time, the elastic force so that the bottom of the channel cover part 240 is in close contact with the support wall 340. Let's go.
  • channel cover portion 240 may be in close contact with the support wall 340 elastically by each tension generating connector 260.
  • a volume 400 having a constant volume is formed between the channel cover part 240 and the channel part 330. That is, since the channel cover part 240 is in close contact with the support wall 340 while being stretched in all directions by the tensile force of the tension generating connector 260, there is no deformation of the channel cover part 240 and the contact surface with the support wall 340. Since the channel 400 is formed inside the volume is uniform.
  • a solution of a sample is injected into the inlet 220.
  • the injected solution is temporarily accommodated in the reservoir portion 350 and then moves to the channel 400 formed between the channel cover portion 240 and the channel portion 330 by capillary force along the inclined surface 360.
  • Figure 16 is a partially enlarged cross-sectional view showing another embodiment of the coupling means shown in FIG.
  • another embodiment of the coupling means provided in the above-described embodiment includes at least one post 280 protruding from the bottom surface such that the coupling means are spaced apart from each other along the bottom circumference of the first joint part 250.
  • At least one post insertion hole 380 is formed in the second bonding portion 310 at a position corresponding to the post 280 so that the post 280 can be inserted therethrough.
  • the hook 270 or the end of the post 280 may be formed by engaging the jaw having elasticity to be hooked into the hook insertion groove 370 or the post insertion hole 380 after being caught. .
  • the present invention is not limited to this, but the present invention is not limited thereto.
  • FIG. 17 is a schematic diagram of a sample image analyzing apparatus using the chamber of FIG. 1.
  • the sample image analyzing apparatus 1 may include a quantitative microparticle counting chamber 100 in which a sample is accommodated, and a light source unit for irradiating light to the sample side accommodated in the chamber 100. 2), an objective lens 3 for enlarging an image of the sample formed by the light emitted from the light source unit 2, an image acquisition unit 4 for capturing an image of the specimen enlarged through the objective lens 3, and The image reading unit 5 for reading an image of the sample photographed by the image obtaining unit 4 and the chamber 100 so that a specific region of the chamber 100 to be photographed are at the incidence position of the light source unit 2 are provided. And a moving stage 6 for moving.
  • the chamber 100 is substantially the same as the chamber 100 of the above-described embodiment, redundant description thereof will be omitted.
  • the light source unit 2 is a configuration for supplying light to the sample side accommodated in the chamber 100, and is provided using a light emitting diode (LED) in this embodiment. Since the light source unit 2 provided with the LED has a longer life than a general mercury or xenon arc lamp, the frequency of maintenance work for replacing the light source unit 2 can be reduced, and the amount of light is proportional to the lighting time. Since there is little problem of deterioration, it has the advantage of obtaining an image of a stable sample.
  • LED light emitting diode
  • the light source unit 2 provided with the LED has a small amount of heat generation, it is possible to prevent a phenomenon in which thermal deformation occurs in the sample, and because the size is very small, the fluorescent microscope 100 can be miniaturized.
  • the light source unit 2 may be provided using a general mercury lamp or the like.
  • the object lens 3 is configured to enlarge an image of a sample formed by light emitted from the light source unit 2.
  • the objective lens 3 is provided with a low magnification objective lens 3 having a magnification of about 10 times, but the magnification of the objective lens 3 may be changed as necessary.
  • the image acquisition unit 4 is a configuration for visually realizing an image of the sample enlarged through the objective lens 3.
  • the image acquisition unit 4 is provided with a charge-coupled device (CCD) camera.
  • CCD charge-coupled device
  • the CCD camera reads information from the bottom row of the CCD into the read out register line by line to read the charge stored in each photosite after the CCD is exposed to light.
  • the image acquisition unit 4 provided with the CCD camera has excellent advantages in noise level, noise processing, and quality of the image itself.
  • the image acquisition unit 4 may be provided as a complementary metal oxide semiconductor (CMOS) image sensor.
  • CMOS image sensor refers to a solid-state imaging device using a complementary metal oxide semiconductor (CMOS), which uses a photodiode similarly to a CCD image sensor but differs in manufacturing process and signal reading method.
  • CMOS image sensors have excellent advantages in terms of integration and power consumption.
  • the image reading part 5 is a structure for reading the image of the sample
  • the image reading unit 5 includes a program capable of reading an image so as to read the size of the sample photographed by the image obtaining unit 4, the intensity of fluorescence or the number of cells present in the sample. That is, cells containing a cell nucleus, such as leukocytes or somatic cells, are pre-processed through a fluorescent dye and accommodated in the chamber 100, are photographed by the image acquisition unit 4, and then photographed by the image reading unit 5. Details such as diameter and fluorescence intensity are read. In addition, it is possible to determine the number of cells contained in the entire sample through the diameter of the cells and the intensity of fluorescence.
  • the moving stage 6 is configured to move the chamber 100 in the X-axis direction and the Y-axis direction, respectively, so that a specific region of the chamber 100 is at the incidence position of the light source unit 2.
  • the moving stage 6 moves the chamber 100 in the X-axis or Y-axis direction so that the image acquiring unit 4 can photograph each region of the chamber 100, and the photographed chamber 100 Each region of is combined by the image reading unit 5 into the entire region of the chamber 100 so that the entire image of the sample can be read.
  • the operator who wants to read the image of the sample is to place the chamber 100 containing the sample on the upper surface of the moving stage (6).
  • the light is irradiated toward the chamber 100 through the light source unit 2, and the fluorescent image generated by the irradiated light is enlarged by the objective lens 3 and then reflected by the dichroic filter F1, and again.
  • the image is captured by the image acquisition unit 4 via the radiation filter F2.
  • the dichroic filter F1 and the radiation filter F2 refer to a filter that selectively transmits only light having a specific wavelength.
  • Other details regarding the dichroic filter F1 and the radiation filter F2 are the same as those of the known ones, and thus detailed description thereof will be omitted.
  • the moving stage 6 is moved so that another specific area of the captured chamber 100 is positioned directly below the light source unit 2. This is photographed by repeating the above process.
  • An image of each region of the chamber 100 taken by the image acquisition unit 4 is transmitted to the image reading unit 5 side, based on which the operator has a diameter of the cell and a fluorescence intensity. This can be read.
  • reference numeral E denotes a stepping motor for moving the moving stage 6, and G denotes a piezoelectric motor for adjusting the focal length of the objective lens 3. And, R means a controller for controlling the entire system of the sample image analysis device (1).
  • the sample image analyzing apparatus 1 of the present exemplary embodiment has an advantage of allowing a worker to read a sample image of a proper quantity by using a chamber 100 having a uniform channel volume and height, thereby easily reading a small amount of sample.
  • sample image analysis device 1 of the present embodiment has the advantage that it can be miniaturized while adopting a simple optical structure and utilizing the short moving stroke stage 6 to fulfill the object of the image analysis device. .

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  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne une chambre de comptage pour particules fines en quantité fixe, destinée à fournir un volume constant, ainsi qu'un appareil d'analyse d'images d'échantillon la mettant en oeuvre. Cette chambre de comptage comprend : une première plaque ; une deuxième plaque couplée à la première de sorte à former un canal contenant une solution pour le comptage des particules fines, la première plaque comprenant une partie de recouvrement de canal ; une première partie d'assemblage espacée d'une distance prédéterminée de la circonférence externe de la partie de recouvrement de canal ; et une partie de production de tension et de raccordement qui relie la partie de recouvrement de canal et la première partie d'assemblage, de sorte que la partie de recouvrement de canal vienne en contact élastique et hermétique avec une zone formant un canal sur la deuxième plaque lorsque les première et deuxième plaques sont couplées. Selon l'invention, la partie de recouvrement de canal de la première plaque vient en contact élastique et hermétique avec une paroi de support de la deuxième plaque par la partie de production de tension et de raccordement, ce qui permet de maintenir l'herméticité entre la partie de recouvrement de canal et la paroi de support, ce qui empêche les fuites de la solution contenue dans le canal.
PCT/KR2011/000932 2010-02-12 2011-02-11 Chambre de comptage pour particules fines en quantité fixe et appareil d'analyse d'images d'échantillon mettant en oeuvre cette chambre WO2011099809A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2010-0013277 2010-02-12
KR20100013277 2010-02-12

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WO2011099809A2 true WO2011099809A2 (fr) 2011-08-18
WO2011099809A3 WO2011099809A3 (fr) 2011-12-15

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103733064A (zh) * 2011-08-19 2014-04-16 逻辑生物科技有限公司 微芯片

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WO1996025682A1 (fr) * 1995-02-15 1996-08-22 De Kock, Alfons Petrus Antonius Gerrit Compartiment de comptage pour analyses biologiques, et son procede de fabrication
KR20050009612A (ko) * 2003-07-18 2005-01-25 주식회사 디지탈바이오테크놀러지 세포 개체수 계수용 장치 및 그 제조방법
KR20050010709A (ko) * 2003-07-19 2005-01-28 주식회사 디지탈바이오테크놀러지 미세입자 계수 장치
KR100719238B1 (ko) * 2006-04-10 2007-05-18 에스케이씨 주식회사 마이크로 입자 계수용 플라스틱 마이크로 칩과 그 제조방법
WO2007094817A2 (fr) * 2005-08-02 2007-08-23 University Of Utah Research Foundation biocapteurs comprenant des nanocavités métalliques
US20080019584A1 (en) * 2006-07-19 2008-01-24 Stellan Lindberg Measurement apparatus, method and computer program
KR20080051516A (ko) * 2006-12-06 2008-06-11 에스케이씨 주식회사 방향표식이 부가된 마이크로 입자 계수용 플라스틱마이크로 칩과 그 제조방법

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996025682A1 (fr) * 1995-02-15 1996-08-22 De Kock, Alfons Petrus Antonius Gerrit Compartiment de comptage pour analyses biologiques, et son procede de fabrication
KR20050009612A (ko) * 2003-07-18 2005-01-25 주식회사 디지탈바이오테크놀러지 세포 개체수 계수용 장치 및 그 제조방법
KR20050010709A (ko) * 2003-07-19 2005-01-28 주식회사 디지탈바이오테크놀러지 미세입자 계수 장치
WO2007094817A2 (fr) * 2005-08-02 2007-08-23 University Of Utah Research Foundation biocapteurs comprenant des nanocavités métalliques
KR100719238B1 (ko) * 2006-04-10 2007-05-18 에스케이씨 주식회사 마이크로 입자 계수용 플라스틱 마이크로 칩과 그 제조방법
US20080019584A1 (en) * 2006-07-19 2008-01-24 Stellan Lindberg Measurement apparatus, method and computer program
KR20080051516A (ko) * 2006-12-06 2008-06-11 에스케이씨 주식회사 방향표식이 부가된 마이크로 입자 계수용 플라스틱마이크로 칩과 그 제조방법

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103733064A (zh) * 2011-08-19 2014-04-16 逻辑生物科技有限公司 微芯片

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