WO2011097420A3 - Selection and characterization of dna aptamers with binding selectivity to campylobacter jejuni using whole-cell selex - Google Patents

Selection and characterization of dna aptamers with binding selectivity to campylobacter jejuni using whole-cell selex Download PDF

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Publication number
WO2011097420A3
WO2011097420A3 PCT/US2011/023656 US2011023656W WO2011097420A3 WO 2011097420 A3 WO2011097420 A3 WO 2011097420A3 US 2011023656 W US2011023656 W US 2011023656W WO 2011097420 A3 WO2011097420 A3 WO 2011097420A3
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WO
WIPO (PCT)
Prior art keywords
dna aptamers
characterization
selection
whole
campylobacter jejuni
Prior art date
Application number
PCT/US2011/023656
Other languages
French (fr)
Other versions
WO2011097420A2 (en
Inventor
Lee-Ann Jaykus
Hari Prakash Dwivedi
Ronald Derike Smiley
Original Assignee
North Carolina State University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by North Carolina State University filed Critical North Carolina State University
Publication of WO2011097420A2 publication Critical patent/WO2011097420A2/en
Publication of WO2011097420A3 publication Critical patent/WO2011097420A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

There is disclosed DNA aptamers demonstrating binding specificity to live Campylobacer jejuni cells and a method of using the DNA aptamers for capture, concentration, and detection of pathogens in simple and complex sample matrices, including but not limited to food and environmental samples.
PCT/US2011/023656 2010-02-03 2011-02-03 Selection and characterization of dna aptamers with binding selectivity to campylobacter jejuni using whole-cell selex WO2011097420A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US30100010P 2010-02-03 2010-02-03
US61/301,000 2010-02-03

Publications (2)

Publication Number Publication Date
WO2011097420A2 WO2011097420A2 (en) 2011-08-11
WO2011097420A3 true WO2011097420A3 (en) 2011-10-13

Family

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Family Applications (1)

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PCT/US2011/023656 WO2011097420A2 (en) 2010-02-03 2011-02-03 Selection and characterization of dna aptamers with binding selectivity to campylobacter jejuni using whole-cell selex

Country Status (1)

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WO (1) WO2011097420A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013064818A1 (en) * 2011-10-31 2013-05-10 Dupont Nutrition Biosciences Aps Aptamers
AU2013205090B2 (en) * 2012-12-07 2016-07-28 Gen-Probe Incorporated Compositions and Methods for Detecting Gastrointestinal Pathogen Nucleic Acid
KR102059413B1 (en) 2018-05-17 2019-12-26 전북대학교산학협력단 Composition for detecting Campylobacter comprising Campylobacter specific aptamer and nanoparticle and use thereof
CN113278622B (en) * 2021-06-09 2023-09-19 集美大学 Pseudomonas deformans aptamer and screening method thereof
EP4382621A3 (en) * 2021-07-27 2024-08-21 Gen-Probe Incorporated Compositions and methods for detecting c. coli and at least one of salmonella, c. jejuni, shigella, and stec

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090186342A1 (en) * 2006-05-12 2009-07-23 Pronucleotein Biotechnologies, Llc Methods of producing competitive aptamer fret reagents and assays

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090186342A1 (en) * 2006-05-12 2009-07-23 Pronucleotein Biotechnologies, Llc Methods of producing competitive aptamer fret reagents and assays

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
DATABASE GENBANK [online] 1 February 2004 (2004-02-01), "C.neoformans strain JEC21 Cryptococcus neoformans var. neoformans cDNA clone CCAC318, mRNA sequence.", retrieved from http://www.ncbi.nlm.nih.gov/nucest/CF711523 Database accession no. CF711523.1 *
DWIVEDI ET AL.: "Selection and characterization of DNA aptamers with binding selectivity to Campylobacterjejuni using whole-cell SELEX.", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 87, no. 6, 27 June 2010 (2010-06-27), pages 2323 - 2334 *
DWIVEDI: "Development of Molecular-based Methods to Capture and Detect Salmonella and Campylobacter in Complex Sample Matrices", PHD THESIS, 4 January 2010 (2010-01-04), Retrieved from the Internet <URL:http://repository.lib.ncsu.edu/irlbitstream/1840.16/6634/1/etd.pdf> [retrieved on 20110430] *
SOOTER ET AL.: "Application of capillary electrophoresis to the development and evaluation of aptamer affinity probes", PROCEEDINGS OF SPIE, vol. 6759, 2007, pages 67590T1 - 67590T8 *
STRATIS-CULLUM ET AL.: "Affinity Probe Capillary Electrophoresis Evaluation of Aptamer Binding to Campylobacter jejuni Bacteria.", ARL-TR-5015, November 2009 (2009-11-01), pages 8, Retrieved from the Internet <URL:http://www.dtic.mil/cgi-biNGetTRDoc?Location=U28doc=GetTRDoc.pdfBAD=ADA512469> [retrieved on 20110430] *

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Publication number Publication date
WO2011097420A2 (en) 2011-08-11

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