WO2011090793A3 - Multiplex microarray of serially deposited biomolecules on a microarray - Google Patents

Multiplex microarray of serially deposited biomolecules on a microarray Download PDF

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Publication number
WO2011090793A3
WO2011090793A3 PCT/US2011/000112 US2011000112W WO2011090793A3 WO 2011090793 A3 WO2011090793 A3 WO 2011090793A3 US 2011000112 W US2011000112 W US 2011000112W WO 2011090793 A3 WO2011090793 A3 WO 2011090793A3
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WO
WIPO (PCT)
Prior art keywords
microarray
multiplex
electrodes
functionalized
serially
Prior art date
Application number
PCT/US2011/000112
Other languages
French (fr)
Other versions
WO2011090793A2 (en
Inventor
John Cooper
Original Assignee
Customarray, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Customarray, Inc. filed Critical Customarray, Inc.
Publication of WO2011090793A2 publication Critical patent/WO2011090793A2/en
Publication of WO2011090793A3 publication Critical patent/WO2011090793A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes

Abstract

Disclosed herein is a multiplex microarray having serially attached non-functionalized biomolecules attached to a polymer coating covering each electrode of an array of electrodes for assays and a method of making the multiplex microarray. The method comprises serially blocking the electrodes of the microarray with a blocking protein, electropolymerizing pyrrole or a functionalized pyrrole on the electrodes where the biomolcule is not present during polymerization, exposing the microarray to a biomolecular solution containing a non-functionalized biomolecule for attachment to the polymer coating, and then repeating the steps to form the multiplex microarray.
PCT/US2011/000112 2010-01-20 2011-01-20 Multiplex microarray of serially deposited biomolecules on a microarray WO2011090793A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US33638610P 2010-01-20 2010-01-20
US61/336,386 2010-01-20

Publications (2)

Publication Number Publication Date
WO2011090793A2 WO2011090793A2 (en) 2011-07-28
WO2011090793A3 true WO2011090793A3 (en) 2011-09-15

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PCT/US2011/000112 WO2011090793A2 (en) 2010-01-20 2011-01-20 Multiplex microarray of serially deposited biomolecules on a microarray

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US (2) US9927434B2 (en)
WO (1) WO2011090793A2 (en)

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Also Published As

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US20110281766A1 (en) 2011-11-17
US10591476B1 (en) 2020-03-17
US9927434B2 (en) 2018-03-27
WO2011090793A2 (en) 2011-07-28

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