WO2011063324A3 - Microfluidic systems for isolating microvesicles - Google Patents

Microfluidic systems for isolating microvesicles Download PDF

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Publication number
WO2011063324A3
WO2011063324A3 PCT/US2010/057599 US2010057599W WO2011063324A3 WO 2011063324 A3 WO2011063324 A3 WO 2011063324A3 US 2010057599 W US2010057599 W US 2010057599W WO 2011063324 A3 WO2011063324 A3 WO 2011063324A3
Authority
WO
WIPO (PCT)
Prior art keywords
microfluidic systems
isolating microvesicles
microvesicles
isolating
serum sample
Prior art date
Application number
PCT/US2010/057599
Other languages
French (fr)
Other versions
WO2011063324A2 (en
Inventor
Daniel Irimia
Mehmet Toner
Chihchen Chen
Xandra O. Breakfield
Johan Skog
Original Assignee
The General Hospital Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The General Hospital Corporation filed Critical The General Hospital Corporation
Publication of WO2011063324A2 publication Critical patent/WO2011063324A2/en
Publication of WO2011063324A3 publication Critical patent/WO2011063324A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals

Abstract

Methods, apparatuses, and systems for isolating microvesicles in a serum sample include flowing a serum sample that includes microvesicles and other particles that have been shed from a cell surface, through a microchannel formed in a microfluidic device.
PCT/US2010/057599 2009-11-20 2010-11-22 Microfluidic systems for isolating microvesicles WO2011063324A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US26309709P 2009-11-20 2009-11-20
US61/263,097 2009-11-20

Publications (2)

Publication Number Publication Date
WO2011063324A2 WO2011063324A2 (en) 2011-05-26
WO2011063324A3 true WO2011063324A3 (en) 2011-09-15

Family

ID=44060389

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2010/057599 WO2011063324A2 (en) 2009-11-20 2010-11-22 Microfluidic systems for isolating microvesicles

Country Status (1)

Country Link
WO (1) WO2011063324A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014078420A1 (en) * 2012-11-13 2014-05-22 Allan Wu Methods and systems for processing exosomes
KR101473557B1 (en) * 2013-03-29 2014-12-24 포항공과대학교 산학협력단 Apparatus for the preparation of cell-derived artificial microvesicles using centrifugal force
EP3051293B1 (en) 2013-09-25 2021-11-03 The University of Tokyo Solution mixer, fluid device, and solution mixing method
WO2019067567A1 (en) * 2017-09-26 2019-04-04 Juno Diagnostics, Inc. Devices, systems and methods for biomarker analysis
AR113802A1 (en) 2017-10-27 2020-06-10 Juno Diagnostics Inc DEVICES, SYSTEMS AND METHODS FOR ULTRA-LOW VOLUME LIQUID BIOPSY
CN113008652A (en) * 2021-02-25 2021-06-22 重庆医科大学附属第三医院(捷尔医院） Method for separating exosome by using TIM-4 functionalized fishbone-shaped microfluidic chip

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5811250A (en) * 1993-03-24 1998-09-22 Nycomed Pharma A/S Method of diagnosing haemastatic disorders
WO2007106598A2 (en) * 2006-03-15 2007-09-20 The General Hospital Corporation Devices and methods for detecting cells and other analytes
WO2009015357A1 (en) * 2007-07-25 2009-01-29 University Of Louisville Research Foundation, Inc. Exosome-associated microrna as a diagnostic marker
WO2009100029A1 (en) * 2008-02-01 2009-08-13 The General Hospital Corporation Use of microvesicles in diagnosis, prognosis and treatment of medical diseases and conditions

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5811250A (en) * 1993-03-24 1998-09-22 Nycomed Pharma A/S Method of diagnosing haemastatic disorders
WO2007106598A2 (en) * 2006-03-15 2007-09-20 The General Hospital Corporation Devices and methods for detecting cells and other analytes
WO2009015357A1 (en) * 2007-07-25 2009-01-29 University Of Louisville Research Foundation, Inc. Exosome-associated microrna as a diagnostic marker
WO2009100029A1 (en) * 2008-02-01 2009-08-13 The General Hospital Corporation Use of microvesicles in diagnosis, prognosis and treatment of medical diseases and conditions

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BEAUDOIN A.R. ET AL.: "Detection and Characterization of Microvesicles in the Acinar Lumen and in Juice of Unstimulated Rat Pancreas.", THE JOURNAL OF HISTO CHEMISTRY AND CYTOCHEMISTRY, vol. 34, no. 8, 1986, pages 1079 - 1084 *

Also Published As

Publication number Publication date
WO2011063324A2 (en) 2011-05-26

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