WO2011048111A1 - Compounds - Google Patents

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Publication number
WO2011048111A1
WO2011048111A1 PCT/EP2010/065746 EP2010065746W WO2011048111A1 WO 2011048111 A1 WO2011048111 A1 WO 2011048111A1 EP 2010065746 W EP2010065746 W EP 2010065746W WO 2011048111 A1 WO2011048111 A1 WO 2011048111A1
Authority
WO
WIPO (PCT)
Prior art keywords
methyl
amino
pyrazolo
hydroxyphenyl
pyrimidin
Prior art date
Application number
PCT/EP2010/065746
Other languages
French (fr)
Inventor
John King-Underwood
Kazuhiro Ito
Peter John Murray
George Hardy
Frederick Arthur Brookfield
Christopher John Brown
Original Assignee
Respivert Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=41462531&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO2011048111(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Respivert Limited filed Critical Respivert Limited
Priority to ES10771416.4T priority Critical patent/ES2477270T3/en
Priority to MX2012004525A priority patent/MX2012004525A/en
Priority to CN201080047955.2A priority patent/CN102712645B/en
Priority to SI201030660T priority patent/SI2491037T1/en
Priority to NZ599162A priority patent/NZ599162A/en
Priority to JP2012534671A priority patent/JP5889792B2/en
Priority to PL10771416T priority patent/PL2491037T3/en
Priority to US13/502,465 priority patent/US8741909B2/en
Priority to CA2777902A priority patent/CA2777902C/en
Priority to DK10771416.4T priority patent/DK2491037T3/en
Priority to UAA201203872A priority patent/UA108743C2/en
Priority to BR112012009214A priority patent/BR112012009214A2/en
Priority to KR1020127012826A priority patent/KR101770616B1/en
Priority to RS20140344A priority patent/RS53385B/en
Priority to EP10771416.4A priority patent/EP2491037B1/en
Priority to EA201270575A priority patent/EA020330B3/en
Priority to AU2010309832A priority patent/AU2010309832B2/en
Publication of WO2011048111A1 publication Critical patent/WO2011048111A1/en
Priority to SG2013017470A priority patent/SG188958A1/en
Priority to KR1020137011430A priority patent/KR101857000B1/en
Priority to EA201390579A priority patent/EA026946B1/en
Priority to BR112013009395A priority patent/BR112013009395A2/en
Priority to PCT/GB2011/052015 priority patent/WO2012052753A1/en
Priority to PL11787742T priority patent/PL2630149T3/en
Priority to DK11787742T priority patent/DK2630149T3/en
Priority to EP11787742.3A priority patent/EP2630149B1/en
Priority to CA2811171A priority patent/CA2811171A1/en
Priority to PT117877423T priority patent/PT2630149E/en
Priority to SI201130442T priority patent/SI2630149T1/en
Priority to NZ609147A priority patent/NZ609147A/en
Priority to MX2013004298A priority patent/MX346336B/en
Priority to RS20150206A priority patent/RS53898B1/en
Priority to MYPI2013700611A priority patent/MY164731A/en
Priority to JP2013533289A priority patent/JP5422783B2/en
Priority to AU2011317334A priority patent/AU2011317334B2/en
Priority to ES11787742.3T priority patent/ES2533570T3/en
Priority to MEP-2015-43A priority patent/ME02064B/en
Priority to PE2013000869A priority patent/PE20131464A1/en
Priority to IL219248A priority patent/IL219248A/en
Priority to ZA2012/03662A priority patent/ZA201203662B/en
Priority to HK13100837.2A priority patent/HK1173728A1/en
Priority to IL225212A priority patent/IL225212A/en
Priority to GT201300097A priority patent/GT201300097A/en
Priority to NI201300035A priority patent/NI201300035A/en
Priority to CL2013001057A priority patent/CL2013001057A1/en
Priority to US13/865,379 priority patent/US9340545B2/en
Priority to CO13106722A priority patent/CO6690816A2/en
Priority to ECSP13012612 priority patent/ECSP13012612A/en
Priority to ZA2013/03614A priority patent/ZA201303614B/en
Priority to CR20130233A priority patent/CR20130233A/en
Priority to HK13111905.6A priority patent/HK1184461A1/en
Priority to US14/220,650 priority patent/US9321773B2/en
Priority to HRP20140599AT priority patent/HRP20140599T1/en
Priority to SM201400086T priority patent/SMT201400086B/en
Priority to HRP20150318TT priority patent/HRP20150318T1/en
Priority to CY20151100287T priority patent/CY1116315T1/en
Priority to SM201500076T priority patent/SMT201500076B/en
Priority to US15/086,120 priority patent/US9834560B2/en
Priority to US15/092,707 priority patent/US9637494B2/en
Priority to US15/474,059 priority patent/US10028959B2/en
Priority to US15/806,742 priority patent/US20180118754A1/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
    • C07D487/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains two hetero rings
    • C07D487/04—Ortho-condensed systems
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A61K31/4965—Non-condensed pyrazines
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
    • A61K31/517—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim ortho- or peri-condensed with carbocyclic ring systems, e.g. quinazoline, perimidine
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
    • A61K31/519—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim ortho- or peri-condensed with heterocyclic rings
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/04—Drugs for disorders of the alimentary tract or the digestive system for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/18—Drugs for disorders of the alimentary tract or the digestive system for pancreatic disorders, e.g. pancreatic enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A61P11/02—Nasal agents, e.g. decongestants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A61P11/06—Antiasthmatics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/06—Antipsoriatics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/02—Drugs for skeletal disorders for joint disorders, e.g. arthritis, arthrosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P27/00—Drugs for disorders of the senses
    • A61P27/02—Ophthalmic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A61P35/04—Antineoplastic agents specific for metastasis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/08—Antiallergic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D239/00—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings
    • C07D239/70—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings condensed with carbocyclic rings or ring systems
    • C07D239/72—Quinazolines; Hydrogenated quinazolines
    • C07D239/86—Quinazolines; Hydrogenated quinazolines with hetero atoms directly attached in position 4
    • C07D239/88—Oxygen atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D403/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00
    • C07D403/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings
    • C07D403/06—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings linked by a carbon chain containing only aliphatic carbon atoms

Definitions

  • the invention relates to compounds which are inhibitors of phosphoinositide 3-kinases, (PI3 kinases).
  • PI3 kinases phosphoinositide 3-kinases
  • the invention relates to compounds that inhibit the PI3 kinase delta subtype and optionally, in addition, the gamma and alpha sub-types thereof, and their use in therapy, including in pharmaceutical combinations, especially in the treatment of inflammatory diseases, including inflammatory diseases of the lung, such as COPD and asthma.
  • the disclosure also extends to methods of preparing said compounds and pharmaceutical compositions comprising same.
  • Lipid kinases catalyse the phosphorylation of lipids to produce species involved in the regulation of a wide range of physiological processes, including cellular migration and adhesion.
  • the PI3-kinases belong to this class of enzymes and are membrane associated proteins which catalyse the phosphorylation of lipids which are themselves associated with cell membranes.
  • PI3-kinase delta ( ⁇ ) isozyme (PI3 kinase ⁇ ) is one of four isoforms of type I PI3 kinases responsible for generating various 3'-phosphorylated phosphoinositides, that mediate cellular signalling and have been implicated in a number of cellular processes such as inflammation, growth factor signalling, malignant transformation and immunity (See Review by Rameh, L. E. and Cantley, L. C. J. Biol. Chem., 1999, 274:8347-8350).
  • pan-PI3 kinase inhibitors such as LY-294002 and wortmannin (Ito, K. et al., J Pharmacol. Exp. Ther., 2007, 321 :1 -8).
  • pan-PI3 kinase inhibitors such as LY-294002 and wortmannin (Ito, K. et al., J Pharmacol. Exp. Ther., 2007, 321 :1 -8).
  • pan-PI3 kinase inhibitors such as LY-294002 and wortmannin
  • the PI3 kinase ⁇ selective inhibitor IC-87114 was found to inhibit airway hyper- responsiveness, IgE release, pro-inflammatory cytokine expression, inflammatory cell accumulation into the lung and vascular permeability in ovalbumin-sensitized, ovalbumin- challenged mice [Lee, K. S. et al., J. Allergy Clin. Immunol., 2006, 1 18:403-409 and Lee, K. S. et al., FASEB J., 2006, 20:455-65].
  • IC-87114 lowered neutrophil accumulation in the lungs of mice and neutrophil function, stimulated by TNFa [Sadhu, C. et al., Biochem. Biophys. Res.
  • the PI3 kinase ⁇ isoform is activated by insulin and other growth factors, as well as G-protein coupled protein signaling and inflammatory cytokines.
  • the PI3 kinase dual ⁇ / ⁇ inhibitor TG100-115 was reported to inhibit pulmonary eosinophilia, interleukin-13 as well as mucin accumulation and airways hyperesponsiveness in a murine model, when administered by aerosolisation.
  • the same authors also reported that the compound was able to inhibit pulmonary neutrophilia elicited by either LPS or cigarette smoke [Doukas, J. et al., J Pharmacol. Exp.
  • Akt serine/threonine protein kinase
  • rapamycin the mammalian target of rapamycin
  • CM S alkyl chain is H, a saturated or unsaturated, branched or unbranched CM S alkyl chain, wherein optionally one or more carbons (for example 1 , 2 or 3 carbons, suitably 1 or 2, in particular 1 ) is/are replaced by a heteroatom selected from O, N, S(0) p , (for example a CH 2 , group is replaced with O, or with NH, or with S, or with S0 2 or a -CH 3 group at the terminus of the chain or on a branch is replaced with OH or with NH 2 ) wherein said chain is optionally substituted by one or more groups (for example 1 to 3, such as 2 groups) independently selected from oxo, halogen, an aryl group, a heteroaryl group, a carbocyclyl group or a heterocyclyl group, each aryl, heteroaryl, carbocyclyl or heterocyclyl group bearing:
  • R 2a is selected from hydrogen, -Ci -3 alkyl, halo, hydroxyl, cyano, -Ci -3 haloalkyl, -Ci -3 alkoxy, -C 2-3 alkoxyOCi -3 alkyl, -C 2-3 alkylOCi -3 alkyl, -Ci -3 hydroxyalkyl, -C 0-6 alkylS(0) q Ci -3 alkyl, -C 0-6 alkylS(0) p NR 6 R 7 , -C 0-6 alkylNR 8 C 0- 6 alkylS(0)pCi -6 alkyl, -C 0-6 alkylC(0)OH, -C 0-6 alkylC(0)OCi -6 alkyl, -NR 8 C 0-6 alkylC(0)NR 6 R 7 , -NR 8 C 0-6 alkylC(0)C(0)NR 6 R 7 , -NR 8 C 0-6 alkylC(0)C(0)NR 6 R
  • R 2b is selected from hydrogen, Ci -3 alkyl, halo, hydroxyl, cyano, -Ci -3 haloalkyl, -Ci -3 alkoxy, and -C 0- 6 alkylS(0) q Ci -3 alkyl;
  • R is hydroxyl
  • R 3b is selected from hydrogen, hydroxyl, halo, cyano, -Ci -3 haloalkyl, -Ci -3 hydroxyalkyl, -Ci -3 alkoxy, and -S(0) q Ci -3 alkyl;
  • R 4 is hydrogen or -Ci -3 alkyl
  • R 5 is hydrogen or -Ci -3 alkyl
  • R 6 is hydrogen or -Ci -6 alkyl
  • R 7 is hydrogen or -Ci -6 alkyl
  • R 8 is hydrogen or -Ci -6 alkyl
  • p is 0 or an integer 1 or 2;
  • q is 0 or an integer 1 or 2
  • R 1 is H, a saturated or unsaturated, branched or unbranched alkyl chain, wherein optionally one or more carbons (for example 1 , 2 or 3 carbons, suitably 1 or 2, in particular 1 ) is/are replaced by a heteroatom selected from O, N, S(0) p , (for example a CH 2 , group is replaced with O, or with NH, or with S, or with S0 2 or a -CH 3 group at the terminus of the chain or on a branch is replaced with OH or with NH 2 ) wherein said chain is optionally substituted by one or more groups (for example 1 to 3, such as 2 groups) independently selected from oxo, halogen, an aryl group, a heteroaryl group, a carbocyclyl group or a heterocyclyl group, each aryl, heteroaryl, carbocyclyl or heterocyclyl group bearing:
  • R 1 is H, a saturated or unsaturated, branched or unbranched CMO alkyl chain, wherein optionally one or more carbons (for example 1 , 2 or 3 carbons, suitably 1 or 2, in particular 1 ) is/are replaced by a heteroatom selected from O, N, S(0) p , (for example a
  • CH 2 , group is replaced with O, or with NH, or with S, or with S0 2 or a -CH 3 group at the terminus of the chain or on a branch is replaced with OH or with NH 2 ) wherein said chain is optionally substituted by one or more groups (for example 1 to 3, such as 2 groups) independently selected from oxo, halogen, an aryl group, a heteroaryl group, a carbocyclyl group or a heterocyclyl group, each aryl, heteroaryl, carbocyclyl or heterocyclyl group bearing:
  • R 2b is hydrogen, Ci -3 alkyl, halo, cyano, -Ci -3 haloalkyl, -Ci -3 alkoxy, -C 0- 6 alkylS(0) q Ci -3 alkyl;
  • R 3a is hydroxyl
  • R 3b is hydrogen, hydroxyl, halo, cyano, -Ci -3 haloalkyl, -Ci -3 hydroxyalkyl, -Ci -3 alkoxy, -S(0) q Ci -3 alkyl;
  • R 4 is hydrogen or -Ci -3 alkyl
  • R 5 is hydrogen or -Ci -3 alkyl
  • R 6 is hydrogen or -Ci -6 alkyl
  • R 7 is hydrogen or -Ci -6 alkyl
  • R 8 is hydrogen or -Ci 6 alkyl
  • P is 0 or an integer 1 or 2;
  • q is 0 or an integer 1 or 2
  • the present disclosure provides a compound of formula ( ⁇ '):
  • CM S alkyl chain is H , a saturated or unsaturated, branched or unbranched CM S alkyl chain, wherein optionally one or more carbons (for example 1 , 2 or 3 carbons, suitably 1 or 2, in particular 1 ) is/are replaced by a heteroatom selected from O, N , S(0) p , (for example a CH 2 , group is replaced with O, or with N H , or with S, or with S0 2 or a -CH 3 group at the terminus of the chain or on a branch is replaced with OH or with N H 2 ) wherein said chain is optionally substituted by one or more groups (for example 1 to 3, such as 2 groups) independently selected from oxo, halogen, an aryl group, a heteroaryl group, a carbocyclyl group or a heterocyclyl group, each aryl, heteroaryl, carbocyclyl or heterocyclyl group bearing:
  • R 2a is hydrogen, -Ci -3 alkyl, halo, hydroxyl, cyano, -Ci -3 haloalkyl, -Ci -3 alkoxy, -C 2-3 alkoxyOCi -3 alkyl, -Ci -3 hydroxyalkyl, -C 0-6 alkylS(0) q Ci -3 alkyl, -C 0-6 alkylS(0) p NR 6 R 7 , -C 0-6 alkylNR 8 C 0 -6alkylS(O)pCi -6 alkyl, -C 0-6 alkylC(0)OH, -C 0-6 alkylC(0)OCi -6 alkyl, -NC 0-6 alkylC(0)NR 6 R 7 , -NR 8 C 0-6 alkylC(0)d -6 alkyl, -C 0-6 alkylC(0)NR 6 R 7 and -C 0-6 alkylC(0)Ci -6 alkyl;
  • R 2b is hydrogen, Ci -3 alkyl, halo, cyano, Ci -3 haloalkyl, Ci -3 alkoxy, C 0- 6 alkylS(0) q Ci -3 alkyl;
  • R 3a is hydroxyl;
  • R 3b is hydrogen, hydroxyl, halo, cyano, Ci -3 haloalkyl, Ci -3 hydroxyalkyl, Ci -3 alkoxy, S(0) q Ci -3 alkyl;
  • R 4 is hydrogen or -Ci -3 alkyl
  • R 5 is hydrogen or -Ci -3 alkyl
  • R 6 is hydrogen or -Ci -6 alkyl
  • R 7 is hydrogen or -Ci -6 alkyl
  • R 8 is hydrogen or -Ci 6 alkyl
  • P is 0 or an integer 1 or 2;
  • q is 0 or an integer 1 or 2
  • R 1 is H , a saturated or unsaturated, branched or unbranched CM S alkyl chain (such as a C-i-12 alkyl chain or a CMO alkyl chain), wherein optionally one or more carbons (for example 1 , 2 or 3 carbons, suitably 1 or 2, in particular 1 ) is/are replaced by a heteroatom selected from O, N , S(0) p , (for example a CH 2 , group is replaced with O, or with N H , or with S, or with S0 2 or a -CH 3 group at the terminus of the chain or on a branch is replaced with OH or with N H 2 ) wherein said chain is optionally substituted by one or more groups independently selected from oxo, halogen, an aryl group, a heteroaryl group, a carbocyclyl group or a heterocyclyl group each aryl, heteroaryl, carbocyclyl or heterocyclyl group bearing:
  • R is hydrogen, -Ci -3 alkyl, halo, hydroxyl, cyano, -Ci -3 haloalkyl, -Ci -3 alkoxy, -C 2-3 alkoxyOCi -3 alkyl, -Ci -3 hydroxyalkyl, -C 0-6 alkylS(0) q Ci -3 alkyl, -C 0-6 alkylS(0) p NR 6 R 7 , -C 0-6 alkylNR 8 C 0 -6alkylS(O)pCi -6 alkyl, -C 0-6 alkylC(0)OH, -C 0-6 alkylC(0)OCi -6 alkyl, -NC 0-6 alkylC(0)NR 6 R 7 , -NR 8 C 0-6 alkylC(0)Ci -6 alkyl, -C 0-6 alkylC(0)NR 6 R 7 and -C 0-6 alkylC(0)Ci -6 alkyl;
  • R 2b is hydrogen, Ci -3 alkyl, halo, cyano, Ci -3 haloalkyl, Ci -3 alkoxy, C 0- 6 alkylS(0) q Ci -3 alkyl;
  • R 3a is hydroxyl;
  • R 3b is hydrogen, hydroxyl, halo, cyano, Ci -3 haloalkyl, Ci -3 hydroxyalkyl, Ci -3 alkoxy, S(0) q Ci -3 alkyl;
  • R 4 is hydrogen or -Ci -3 alkyl
  • R 5 is hydrogen or -Ci -3 alkyl
  • R 6 is hydrogen or -Ci -6 alkyl
  • R 7 is hydrogen or -Ci -6 alkyl
  • R 8 is hydrogen or -Ci 6 alkyl
  • p is 0 or an integer 1 or 2;
  • q is 0 or an integer 1 or 2
  • H a saturated or unsaturated, branched or unbranched C MS alkyl chain, wherein optionally one or more carbons (for example 1 , 2 or 3 carbons, suitably 1 or 2, in particular 1 ) is/are replaced by a heteroatom selected from O, N , S(0) P , (for example a CH 2 , group is replaced with O, or with NH , or with S, or with S0 2 or a -CH 3 group at the terminus of the chain or on a branch is replaced with OH or with NH 2 ) wherein said chain is optionally substituted by one or more groups (for example 1 to 3, such as 2 groups) independently selected from oxo, halogen, an aryl group, a heteroaryl group, a carbocyclyl group or a heterocyclyl group, each aryl, heteroaryl, carbocyclyl or heterocyclyl group bearing:
  • R 2a is hydrogen, -Ci -3 alkyl, halo, hydroxyl, cyano, -Ci -3 haloalkyl, -Ci -3 alkoxy, -C 2-3 alkoxyOCi -3 alkyl, -Ci -3 hydroxyalkyl, -C 0-6 alkylS(0) q Ci -3 alkyl, -C 0-6 alkylS(0) p NR 6 R 7 , -C 0-6 alkylNR 8 C 0 -6alkylS(O)pCi -6 alkyl, -C 0-6 alkylC(0)OH, -C 0-6 alkylC(0)OCi -6 alkyl, -NC 0-6 alkylC(0)NR 6 R 7 , -NR 8 C 0-6 alkylC(0)Ci -6 alkyl, -C 0-6 alkylC(0)NR 6 R 7 and -C 0-6 alkylC(0)Ci -6 alkyl
  • R 3a is hydroxyl
  • R 4 is hydrogen or -Ci -3 alkyl
  • R 5 is hydrogen or-Ci -3 alkyl
  • R 6 is hydrogen or -Ci -6 alkyl
  • R 7 is hydrogen or -Ci -6 alkyl
  • R 8 is hydrogen or -Ci 6 alkyl; p is 0 or an integer 1 or 2;
  • q is 0 or an integer 1 or 2
  • R 1 is H, a saturated or unsaturated, branched or unbranched CM S alkyl chain (such as a C-i-12 alkyl chain or a CMO alkyl chain), wherein optionally one or more carbons (for example 1 , 2 or 3 carbons, suitably 1 or 2, in particular 1 ) is/are replaced by a heteroatom selected from O, N, S(0) p , (for example a CH 2 , group is replaced with O, or with NH, or with S, or with S0 2 or a -CH 3 group at the terminus of the chain or on a branch is replaced with OH or with NH 2 ) wherein said chain is optionally substituted by one or more groups independently selected from oxo, halogen, an aryl group, a heteroaryl group, a carbocyclyl group or a heterocyclyl group each aryl, heteroaryl, carbocyclyl or heterocyclyl group bearing:
  • R 2a is hydrogen, -Ci -3 alkyl, halo, hydroxyl, cyano, -Ci -3 haloalkyl, -Ci -3 alkoxy, -C 2-3 alkoxyOCi -3 alkyl, -Ci -3 hydroxyalkyl, -C 0-6 alkylS(0) q Ci -3 alkyl, -C 0-6 alkylS(0) p NR 6 R 7 , -C 0-6 alkylNR 8 C 0 -6alkylS(O)pCi -6 alkyl, -C 0-6 alkylC(0)OH, -C 0-6 alkylC(0)OCi -6 alkyl, -NC 0-6 alkylC(0)NR 6 R 7 , -NR 8 C 0-6 alkylC(0)Ci -6 alkyl, -C 0-6 alkylC(0)NR 6 R 7 and -C 0-6 alkylC(0)Ci -6 alkyl
  • R 3a is hydroxyl
  • R 4 is hydrogen or -Ci -3 alkyl
  • R 5 is hydrogen or -Ci -3 alkyl
  • R 6 is hydrogen or -Ci -6 alkyl
  • R 7 is hydrogen or -Ci -6 alkyl
  • R 8 is hydrogen or -Ci 6 alkyl
  • p is 0 or an integer 1 or 2;
  • q is 0 or an integer 1 or 2
  • R 1 , R 2a , R 2b , R 3a , R 3b , R 4 and R 5 are as defined above for compounds of formula (I).
  • IE compound of formula
  • R 1 , R 2a , R 3a , R 3b , R 4 and R 5 are as defined above for compounds of formula (I).
  • R 1 , R 2a , R 3a , R 3b , R 4 and R 5 are as defined above for compounds of formula (I).
  • the compounds of the present disclosure are at least PI3K delta inhibitors.
  • Certain compound of the disclosure may be PI3K alpha and delta inhibitors.
  • Certain compounds of the disclosure may be PI3K delta and gamma inhibitors.
  • Some compounds may be alpha and gamma isozyme inhibitors.
  • Other compounds may be alpha, delta and gamma subtype inhibitors. It is proposed that different biological profiles may be associated with these different inhibition profiles which may be advantageous in targeting pathways for different diseases.
  • Inhibitor as employed here is intended to refer to a compound that reduces (for example by at least 50%) or eliminates the biological activity of the target, for example PI3K delta, in an in vitro enzyme assay.
  • the compounds of the present disclosure are active in cell based screening systems and thereby demonstrate that they possess suitable properties for penetrating cells.
  • Alkyl as used herein refers to straight chain or branched chain alkyl, such as, without limitation, methyl, ethyl, propyl, /so-propyl, butyl, and ie f-butyl.
  • alkyl refers to straight chain alkyl.
  • Alkoxy as used herein refers to straight or branched chain alkoxy, for example methoxy, ethoxy, propoxy, butoxy.
  • Alkoxy as employed herein also extends to embodiments in which the oxygen atom is located within the alkyl chain, for example -(CH 2 )nOCH 3 .
  • the alkoxy is linked through oxygen to the remainder of the molecule.
  • the alkoxy is linked through carbon to the remainder of the molecule.
  • the disclosure relates to straight chain alkoxy.
  • -C2-3 alkoxyOCi-3 alkyl as employed herein is intended to refer an -C2-3 alkoxy linked to the remaider of the molecule through oxygen to provide oxygen-alkylene-oxygen-alkyl.
  • Carbocyclyl as employed herein is intended to refer to C3-10 saturated or partially saturated carbocyclic ring systems, for example cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl.
  • Heteroaryl is a C 5 - 9 membered aromatic carbocylic ring or bicyclic ring system comprising one or more, (for example 1 , 2, 3 or 4) heteroatoms independently selected from O, N and S.
  • heteroaryls include: pyrrole, oxazole, thiazole, isothiazole, imidazole, pyrazole, isoxazole, pyridazine, pyrimidine, pyrazine, benzothiophene, benzofuran, or 1 , 2, 3 and 1 , 2, 4 triazole.
  • a bicyclic ring system the definition of heteroaryl will be satisfied if at least one ring contains a heteroatom and at least one ring is aromatic.
  • the heteroaryl may be linked to the remainder of the molecule through a carbocyclic ring or a ring comprising a heteroatom.
  • Heterocyclic groups as employed herein is intended to refer to a 5 to 10 membered ring system which is saturated or partially unsaturated and which is non-aromatic comprising one or more (for example 1 , 2 or 3 in particular 1 or 2,) heteroatoms independently selected from O, N and S, for example 5 or 6 or 7 membered rings including pyrrolidine, tetrahydrofuran, tetrahydrothiophene, piperidine, piperazine, morpholine, 1 ,4-dioxane, pyrrolidine and oxoimidazolidine such as pyrrolidine, tetrahydrofuran, tetrahydrothiophene, piperidine, piperazine, morpholine, and 1 ,4-dioxane, and in particular piperidine, piperazine, and morpholine.
  • pyrrolidine tetrahydrofuran, tetrahydrothiophene
  • piperidine piperazine,
  • An example of a saturated 7 membered heterocyclic ring is a 1 ,4-diazepane ring and an example of a partially unsaturated 7 membered ring is a 2,3,4,5-tetrahydro-1 ,4- oxazepine.
  • a heterocyclic group may be linked to, for example, an alkyl chain, through carbon or a suitable heteroatom such as N.
  • Haloalkyi as employed herein is intended to refer to a group having from 1 to 6 halo atoms, for example 1 to 5 halo atoms.
  • the haloalkyi is per haloalkyi, for example per fluoroalkyl, such as CF 2 CF 3 and CF 3 .
  • Ci-4 mono or C 2-8 di acyl is intended to refer to -C(0)Ci -4 alkyl and -(COCi -4 alkyl) 2 respectively.
  • Aryl as used herein refers to, for example C 6- i 4 mono or polycyclic groups (such as C 6- io mono or bicyclic groups) having from 1 to 3 rings wherein at least one ring is aromatic including phenyl, napthyl, anthracenyl, 5,6,7,8-tetrahydronapthyl and the like, such as phenyl and napthyl.
  • X is phenyl. In one embodiment X is a C 5 - 9 membered heteroaryl.
  • X is pyrazinyl, such as pyrazin-2-yl, in particular methylpyrazin-2-yl, more specifically 5-methylpyrazin-2-yl.
  • X is benzothiophenyl, for example benzothiophen-2-yl.
  • X is benzofuranyl, for example benzofura-5-yl.
  • X is thiazolyl, for example thiazol-4-yl.
  • X is isoxazolyl, for example isoxazol-3-yl, such as 5-methylisoxazol-3-yl.
  • a suitable heteroatom may replace any carbon including a linking carbon, branched carbon or a terminal carbon and hydrogen(s) may fill the remaining valencies, as appropriate.
  • R 1 is H.
  • R 1 is branched or unbranched CMO alkyl chain, wherein at least one carbon (for example 1 , 2 or 3 carbons) is replaced by an oxygen, for example C 9 alkyl comprising 1 , 2 or 3 oxygen atoms, such as -CH2OCH2CH2OCH2CH2OCH 3 .
  • R 1 is -CH 2 OCH 3 .
  • R 1 is -CH2CH2CH2OH. In one embodiment R 1 is -CH2OCH2CH2OCH2CH2OH. In one embodiment R 1 bears at least one oxo substituent. In one embodiment R 1 is -CH 2 NHCONH 2 .
  • R 1 bears at least one carbocyclic group as a substitutent.
  • R 1 bears at least one oxo substituent and a carbocyclic group substituent, for example cyclopentyl or cyclohexyl linked to a carbon bearing an oxo substituent, such as the fragment: one embodiment R 1 comprises
  • R 1 comprises:
  • R 1 comprises:
  • R 1 comprises: In one embodiment R 1 bears at least one heterocyclic group substituent.
  • R 1 bears one oxo substituent and one heterocyclic group substituent, for example the heterocyclic group is linked to a carbon bearing an oxo substituent, such as R 1 comprising -C(0)morpholinyl wherein the morpholinyl may be linked through N, in particular the fragment:
  • R 1 bears one oxo substituent and one heterocyclic group substituent, for example the heterocyclic group is linked to a carbon bearing an oxo substituent, wherein said heterocyclic group itself bears a Ci -6 alkyl or a -C(0)Ci -6 alkyl substitutent, such as R 1 comprising -C(0)piperidinylCi -6 alkyl or -C(0)piperidinylC(0)Ci -6 alkyl wherein the piperidinyl may be linked through N, in particular the fragments:
  • R 1 represent
  • R 1 represents:
  • R1 represents:
  • R 1 bears one oxo substituent and one heterocyclic group substituent, for example the heterocyclic group is linked to a carbon bearing an oxo substituent, wherein said heterocyclic group itself bears a heterocyclic group as a substituent thereon, such as R 1 comprising -C(0)piperidinylmorpholinyl wherein the morpholinyl may be linked through N, in particular the fragment:
  • R 1 comprises:
  • R 1 comprises:
  • R 1 comprises:
  • R 1 represents:
  • R 1 represents:
  • R 1 represents:
  • R 1 represents:
  • R 1 represents:
  • R 1 represents:
  • R 1 represents:
  • R 1 represents:
  • R 1 represents:
  • R 1 represents:
  • R 1 represents:
  • R 1 represents:
  • R 1 represents:
  • R 1 comprises a carboxylic acid, for example Ci -3 alkyleneC0 2 H such as the fragment -CH2CH2CH2CO2H or alternatively -CH2CH2CH2CH2CO2H.
  • R 1 represents -CH2CH2CH 2 C(0)NHCH(CI-l3)2.
  • R 1 represents -CH2CH 2 CH 2 C(0)(CH3)2.
  • R 1 represents -CH2CH2CH2NHCH2CH2OCH 3 . In one embodiment R 1 represents -CH2CH2CH2N(CH2CH 2 OCH3)2. In one embodiment R 1 represents -CH 2 CH2CH2C(0)N(CH2CH 2 OCH3)2. In one embodiment R 1 represents -CH2NHC(0)CH 2 NHC(0)CH3. In one embodiment R 1 represents -CH2CH2CH2C(0)NHCH2CH 2 OCI-l3.
  • R 1 comprises: one embodiment R 1 comprises In one embodiment R 1 comprises: In one embodiment R 1 represents: In one embodiment R 1 represents:
  • R is located in the ortho position. In one embodiment R 2a is located in the meta position. In one embodiment R 2a is located in the para position.
  • R 2a is Ci -3 alkyl, halo, cyano, Ci -3 haloalkyl, Ci -3 alkoxy, S(0) q Ci -3 alkyl, -C(0)OCi-6 alkyl, -NC(0)NCi -6 alkyl, -NC(0)Ci -6 alkyl, -C(0)NCi -6 alkyl, and -C(0)Ci -6 alkyl.
  • R 2a is selected from the group comprising methyl, chloro, fluoro, cyano, methoxy, trifluoromethyl and S0 2 CH 3 . In one embodiment R 2a is chloro.
  • R 2a is fluoro
  • R 2a is cyano
  • R 2a is methoxy. In one embodiment R 2a is methyl. In one embodiment R 2a is S0 2 CH 3 .
  • R 2a is -C(0)OCH 3 . In one embodiment R 2a is CF 3 .
  • R 2b is hydrogen. In one embodiment R 2b is chloro. In one embodiment R 2b is -OCH 3 . In one embodiment R are R are in the 2,3 or 2,4 or 3,4, or 3,5 positions respectively. In one embodiment R 2a is CF 3 and R 2b is methoxy.
  • R 3a is suitably in the ortho, meta or para position, such as the ortho or para position.
  • R 3b is hydrogen.
  • R 3b is fluoro or chloro, for example in the 4-position.
  • R 3a and R 3b are in the 3,4 position respectively.
  • R 4 is H.
  • R 5 is H.
  • p is 2. In one embodiment q is 2.
  • the pharmaceutically acceptable acid addition salts as mentioned hereinabove are meant to comprise the therapeutically active non-toxic acid addition salt forms that the compounds of formula (I) are able to form.
  • These pharmaceutically acceptable acid addition salts can conveniently be obtained by treating the base form with such appropriate acid.
  • Appropriate acids comprise, for example, inorganic acids such as hydrohalic acids, e.g. hydrochloric or hydrobromic acid, sulfuric, nitric, phosphoric and the like acids; or organic acids such as, for example, acetic, propanoic, hydroxyacetic, lactic, pyruvic, oxalic (i.e.
  • ethanedioic malonic
  • succinic i.e. butanedioic acid
  • maleic fumaric, malic, tartaric, citric, methanesulfonic, ethanesulfonic, benzenesulfonic, p-toluenesulfonic, cyclamic, salicylic, p-aminosalicylic, pamoic and the like acids.
  • salts of compound (I) include all pharmaceutically acceptable salts, such as, without limitation, acid addition salts of mineral acids such as HCI and HBr salts and addition salts of organic acids such as a methansulfonic acid salt.
  • the disclosure also extends to solvates of the compounds herein. Examples of solvates include hydrates.
  • the compounds of the disclosure include those where the atom specified is a naturally occurring or non-naturally occurring isotope. In one embodiment the isotope is a stable isotope.
  • the compounds of the disclosure include, for example those containing one or more deuterium atoms in place of hydrogen atoms and the like.
  • the compounds described herein may include one or more chiral centres, and the disclosure extends to include racemates, both enantiomers (for example each substantially free of the other enantiomer) and all stereoisomers resulting therefrom.
  • one enantiomeric form is present in a purified form that is substantially free of the corresponding entaniomeric form.
  • a compound of formula (I) is:
  • Suitable catalysts include palladium catalysts such as t»/s(triphenylphosphine)palladium (II) dichloride, in the presence of copper iodide.
  • a suitable polar aprotic solvent is DMF.
  • a suitable inert atmosphere is nitrogen.
  • the reaction may be performed employing irradiation, for example using microwave irradiation at 120°C and a power setting of 200W.
  • the deprotection, to reveal a compound of formula (I) where, for example R 1 is H, and the protective group is a silyl group can be effected by treatment with a reagent such as tetrabutylammonium chloride in the presence of a polar aprotic solvent such as DMF.
  • a reagent such as tetrabutylammonium chloride
  • a polar aprotic solvent such as DMF.
  • the reaction may be performed at a reduced temperature, such as about 0°C.
  • LGi and X are as defined above for compounds of formula (II) and LG 2 is a leaving group, for example halo, such as chloro, with a compound of formula (V):
  • R 3a , R 3b , R 4 and R 5 are as defined above for compounds of formula (I), in the presence of a base, in a polar aprotic solvent.
  • Suitable protecting groups for compounds of formula (V) in which R is hydroxyl include a ie fbutyldimethylsilylether.
  • Suitable bases include potassium carbonate.
  • a suitable polar aprotic solvent is DMF.
  • LG-i , X, R 4 and R 5 are as defined above for compounds of formula (II) and LG 3 represents a leaving group such as halo, in particular iodo, with a compound of formula (VII):
  • a suitable catalyst is tetrakis(triphenylphosphine)palladium(0).
  • a suitable inorganic base is sodium carbonate.
  • a suitable polar protic solvent is ethanol.
  • a suitable inorganic base is sodium carbonate.
  • a suitable polar aprotic solvent is DMF.
  • LGi is as defined above for compounds of formula (II) and LG 4 is a leaving group, for example halo, such as chloro, or hydroxy with a compound of formula (X): or a protected derivative thereof, wherein X, R and R are as defined above for compounds of formula (II), in the presence of a suitable reagent, such as a phosphorus trihalide, an organic base and a non-polar solvent.
  • a suitable reagent such as a phosphorus trihalide, an organic base and a non-polar solvent.
  • a suitable phosphorus trihalide is phosphorus trichloride.
  • a suitable non-polar solvent is toluene.
  • a suitable organic base is triethylamine.
  • Protection of the hydroxyl may, for example, be effected using TBDMSCI in a suitable solvent, for example DMF in the presence of a suitable base such as imidazole.
  • LG 5 and LG 6 are leaving groups, for example halo, such as chloro in presence of a suitable solvent and a suitable base .
  • a suitable solvent is toluene and a suitable base is pyridine.
  • Protecting groups may be required to protect chemically sensitive groups during one or more of the reactions described above, to ensure that the process is efficient. Thus if desired or necessary, intermediate compounds may be protected by the use of conventional protecting groups. Protecting groups and means for their removal are described in "Protective Groups in Organic Synthesis", by Theodora W. Greene and Peter G.M. Wuts, published by John Wiley & Sons Inc; 4 th Rev Ed., 2006, ISBN-10: 0471697540.
  • novel intermediates are claimed as an aspect of the invention.
  • the compounds of formulae (III), (VII), (VIII), (X) and (XI) and (XII) as defined above, are either commercially available or can be readily prepared from commericially available starting materials, using routine published techniques (see for example Table 1 ).
  • a selection of the compounds of formula (I), disclosed herein, have been screened by chiral stationary phase HPLC for the presence of non interconverting rotational isomers, otherwise known as atropisomers. Atropisomerism results from hindered rotation about a single bond where the torsional energy barrier to free rotation is sufficiently high to significantly slow the interconversion of non-superimposable conformers.
  • the current disclosure provides for compounds that advantageously exist as single discrete molecular entities.
  • the compounds of the present disclosure are particularly useful as therapeutically active agents in pharmaceutical compositions and in treatment.
  • the compounds are useful in treatment, for example COPD and/or asthma.
  • the PI3K compounds developed to date have typically been intended for oral administration.
  • this strategy involves the optimisation of a compound's pharmacokinetic profile in order to achieve an adequate duration of action. In this way a sufficiently high drug concentration is established and maintained between doses to provide continuous clinical benefit.
  • An inevitable and frequently undesired consequence of this approach is that non- targeted body tissues, especially the liver and the gut, are likely to be exposed to pharmacologically active concentrations of the drug.
  • the desired efficacy can sometimes be achieved by ensuring that the drug has a sustained duration of action and is retained predominantly in the target organ, thereby minimising the risks of systemic toxicity.
  • an appropriate formulation can be used which generates a "reservoir" of the active drug which is then available to sustain the desired effects.
  • the first approach is exemplified in the use of the anticholinergic drug tiotropium bromide (Spiriva HandiHaler®), which is administered topically to the lung as a treatment for COPD.
  • This compound has an exceptionally high affinity for its target receptor resulting in a very slow off rate (dissociation rate) and a consequent sustained duration of action.
  • a compound of formulation as a PI3 kinase inhibitor, for example administered topically to the lung.
  • the compounds herein are particularly suitable for topical delivery, such as topical delivery to the lungs, in particular for the treatment of COPD.
  • the compounds are suitable for sensitizing patients to treatment with a corticosteroid.
  • the compounds herein may also be useful for the treatment of rheumatoid arthritis.
  • the present invention provides a pharmaceutical composition
  • a pharmaceutical composition comprising a compound according to the disclosure optionally in combination with one or more pharmaceutically acceptable diluents or carriers.
  • Diluents and carriers may include those suitable for parenteral, oral, topical, mucosal and rectal administration, and may be different depending on the route of administration.
  • compositions may be prepared e.g. for parenteral, subcutaneous, intramuscular, intravenous, intra-articular or peri-articular administration, particularly in the form of liquid solutions or suspensions; for oral administration, particularly in the form of tablets or capsules; for topical e.g. pulmonary or intranasal administration, particularly in the form of powders, nasal drops or aerosols and transdermal administration; for mucosal administration e.g. to buccal, sublingual or vaginal mucosa, and for rectal administration e.g. in the form of a suppository.
  • compositions may conveniently be administered in unit dosage form and may be prepared by any of the methods well-known in the pharmaceutical art, for example as described in Remington's Pharmaceutical Sciences, 17th ed., Mack Publishing Company, Easton, PA., (1985).
  • Formulations for parenteral administration may contain as excipients sterile water or saline, alkylene glycols such as propylene glycol, polyalkylene glycols such as polyethylene glycol, oils of vegetable origin, hydrogenated naphthalenes and the like.
  • Formulations for nasal administration may be solid and may contain excipients, for example, lactose or dextran, or may be aqueous or oily solutions for use in the form of nasal drops or metered spray.
  • typical excipients include sugars, calcium stearate, magnesium stearate, pregelatinated starch, and the like.
  • compositions suitable for oral administration may comprise one or more physiologically compatible carriers and/or excipients and may be in solid or liquid form.
  • Tablets and capsules may be prepared with binding agents, for example, syrup, acacia, gelatin, sorbitol, tragacanth, or poly-vinylpyrollidone; fillers, such as lactose, sucrose, corn starch, calcium phosphate, sorbitol, or glycine; lubricants, such as magnesium stearate, talc, polyethylene glycol, or silica; and surfactants, such as sodium lauryl sulfate.
  • binding agents for example, syrup, acacia, gelatin, sorbitol, tragacanth, or poly-vinylpyrollidone
  • fillers such as lactose, sucrose, corn starch, calcium phosphate, sorbitol, or glycine
  • lubricants such as magnesium stearate, talc, polyethylene glycol
  • Liquid compositions may contain conventional additives such as suspending agents, for example sorbitol syrup, methyl cellulose, sugar syrup, gelatin, carboxymethyl-cellulose, or edible fats; emulsifying agents such as lecithin, or acacia; vegetable oils such as almond oil, coconut oil, cod liver oil, or peanut oil; preservatives such as butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT).
  • suspending agents for example sorbitol syrup, methyl cellulose, sugar syrup, gelatin, carboxymethyl-cellulose, or edible fats
  • emulsifying agents such as lecithin, or acacia
  • vegetable oils such as almond oil, coconut oil, cod liver oil, or peanut oil
  • preservatives such as butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT).
  • BHA butylated hydroxyanisole
  • BHT butylated hydroxytoluen
  • Solid oral dosage forms include tablets, two-piece hard shell capsules and soft elastic gelatin (SEG) capsules.
  • SEG soft elastic gelatin
  • a dry shell formulation typically comprises of about 40% to 60% concentration of gelatin, about a 20% to 30% concentration of plasticizer (such as glycerin, sorbitol or propylene glycol) and about a 30% to 40% concentration of water. Other materials such as preservatives, dyes, opacifiers and flavours also may be present.
  • the liquid fill material comprises a solid drug that has been dissolved, solubilized or dispersed (with suspending agents such as beeswax, hydrogenated castor oil or polyethylene glycol 4000) or a liquid drug in vehicles or combinations of vehicles such as mineral oil, vegetable oils, triglycerides, glycols, polyols and surface-active agents.
  • a pharmaceutical composition comprising a compound of the disclosure optionally in combination with one or more topically acceptable diluents or carriers.
  • Topical administration to the lung may be achieved by use of an aerosol formulation.
  • Aerosol formulations typically comprise the active ingredient suspended or dissolved in a suitable aerosol propellant, such as a chlorofluorocarbon (CFC) or a hydrofluorocarbon (HFC).
  • suitable CFC propellants include trichloromonofluoromethane (propellant 1 1 ), dichlorotetrafluoromethane (propellant 1 14), and dichlorodifluoromethane (propellant 12).
  • Suitable HFC propellants include tetrafluoroethane (HFC-134a) and heptafluoropropane (HFC- 227).
  • the propellant typically comprises 40% to 99.5% e.g. 40% to 90% by weight of the total inhalation composition.
  • the formulation may comprise excipients including co-solvents (e.g. ethanol) and surfactants (e.g. lecithin, sorbitan trioleate and the like). Aerosol formulations are packaged in canisters and a suitable dose is delivered by means of a metering valve (e.g. as supplied by Bespak, Valois or 3M).
  • a metering valve e.g. as supplied by Bespak, Valois or 3M.
  • Topical administration to the lung may also be achieved by use of a non-pressurised formulation such as an aqueous solution or suspension. This may be administered by means of a nebuliser. Topical administration to the lung may also be achieved by use of a dry-powder formulation.
  • a dry powder formulation will contain the compound of the disclosure in finely divided form, typically with a mass mean diameter (MMAD) of 1 -10 ⁇ .
  • the formulation will typically contain a topically acceptable diluent such as lactose, usually of large particle size e.g. a mass mean diameter (MMAD) of 100 ⁇ or more.
  • Example dry powder delivery systems include SPINHALER, DISKHALER, TURBOHALER, DISKUS, SKYEHALER, ACCUHALER and CLICKHALER.
  • a compound of the present is provided as a micronized dry powder formulation, for example comprising lactose of a suitable grade, filled into a device such as DISKUS.
  • Compounds according to the disclosure are intended to have therapeutic activity.
  • the present invention provides a compound of the disclosure for use as a medicament.
  • Compounds according to the disclosure may also be useful in the treatment of respiratory disorders including COPD (including chronic bronchitis and emphysema), asthma, paediatric asthma, cystic fibrosis, sarcoidosis, idiopathic pulmonary fibrosis, allergic rhinitis, rhinitis, sinusitis, especially asthma, chronic bronchitis and COPD.
  • COPD chronic bronchitis and emphysema
  • asthma including chronic bronchitis and emphysema
  • paediatric asthma cystic fibrosis
  • cystic fibrosis sarcoidosis
  • idiopathic pulmonary fibrosis allergic rhinitis
  • rhinitis rhinitis
  • sinusitis especially asthma, chronic bronchitis and COPD.
  • Compounds of the disclosure may also re-sensitise the patient's condition to treatment with a corticosteroid, when the patient's condition has become refractory to the same.
  • One or more compounds of the disclosure may exhibit anti-viral activity and prove useful in the treatment of viral exacerbations of asthma and/or COPD.
  • the compounds of the present disclosure may also be useful in the prophylaxis, treatment or amelioration of flu virus, rhinovirus and/or respiratory syncytical virus.
  • Compounds according to the disclosure are also expected to be useful in the treatment of certain conditions which may be treated by topical or local therapy including allergic conjunctivitis, conjunctivitis, allergic dermatitis, contact dermatitis, psoriasis, ulcerative colitis, inflamed joints secondary to rheumatoid arthritis or osteoarthritis.
  • the compounds of formula (I) are thought to be useful in the, treatment of Hepatitis C and/or HIV, when administered by an appropriate route. Appropriate routes of administration may include oral, intravenous injection or infusion.
  • a compound of formula (I) for the treatment of Hepatitis C is delivered to the blood pre-entry to the liver.
  • Compounds of the disclosure are also expected to be useful in the treatment of certain other conditions including rheumatoid arthritis, pancreatitis, cachexia, inhibition of the growth and metastasis of tumours including non-small cell lung carcinoma, breast carcinoma, gastric carcinoma, colorectal carcinomas and malignant melanoma.
  • the present invention provides a compound as described herein for use in the treatment of one or more of the above mentioned conditions.
  • the present invention provides use of a compound as described herein for the manufacture of a medicament for the treatment of one or more of the above mentioned conditions.
  • the present invention provides a method of treatment of the above mentioned conditions which comprises administering to a subject an effective amount of a compound of the disclosure or a pharmaceutical composition thereof.
  • Compounds described herein may also be used in the manufacture of a medicament for the treatment of one or more of the above-identified diseases.
  • treatment is intended to embrace prophylaxis as well as therapeutic treatment.
  • a compound of the disclosure may also be administered in combination with one or more other active ingredients e.g. active ingredients suitable for treating the above mentioned conditions.
  • active ingredients suitable for treating the above mentioned conditions.
  • possible combinations for treatment of respiratory disorders include combinations with steroids (e.g. budesonide, beclomethasone dipropionate, fluticasone propionate, mometasone furoate, fluticasone furoate), beta agonists (e.g. terbutaline, salbutamol, salmeterol, formoterol, indacaterol) and/or xanthines (e.g. theophylline), musacarinic antagonists, (e.g. ipratropium) and/or a p38 MAP kinase inhibitor.
  • steroids e.g. budesonide, beclomethasone dipropionate, fluticasone propionate, mometasone fur
  • a compound of the disclosure is administered in combination with an antiviral agent, for example acyclovir, tamiflu, relenza or interferon.
  • an antiviral agent for example acyclovir, tamiflu, relenza or interferon.
  • the combination of active ingredients are co-formulated.
  • the combination of active ingredients is simply co-administered.
  • FCS foetal calf serum
  • TLC Thin-layer chromatography
  • Mass spectra were obtained over the range m/z 150 to 850 at a sampling rate of 2 scans per second using Waters ZMD or over m/z 100 to 1000 at a sampling rate of 2 Hz using electrospray ionisation, by a Shimadzu 2010 LC-MS system.
  • Mass spectra were obtained over the range m/z 150 to 850 at a sampling rate of 2 scans per second using Waters ZQ or over m/z 100 to 1000 at a sampling rate of 2 Hz using electrospray ionisation, by a Shimadzu 2010 LC-MS system.
  • UV spectra were recorded at 215 nm using a Waters 2487 dual wavelength UV detector or a Waters 2996 photo diode array. Mass spectra were obtained over the range m/z 150 to 850 at a sampling rate of 2 scans per second using Waters ZMD or a Waters ZQ.
  • Method D was performed on Agilent HP1200, systems using Agilent Extend C18 columns, (1 .8 ⁇ , 4.6 x 30 mm) at 40°C and a flow rate of 2.5-4.5 mL min "1 , eluting with a H 2 0-MeCN gradient containing 0.1 % v/v formic acid over 4 min.
  • Chiral Stationary Phase Analytical HPLC was performed using Daicel columns (5 pm x 5 cm x 4.6 mm) containing one of the following stationary phases: Chiralpak AD-H, Chiralcel OD-H, Chiralcel OJ-H, and Chiralpak AS-H. A flow rate of 1 mL/min, was used eluting under isocratic conditons over 6 to 20 min, with one of the following mobile phases: 70:30 heptane:IPA (v/v), 85:15 heptane:EtOH (v/v), 50:50 MeOH:EtOH (v/v), and acetonitrile; employing UV detection at 254 nm. Each of these mobile phase are compatible with the column packings referred to above, providing sixteen different solvent and stationary phase permutations. Modifers such as triethylamine (0.1 %), formic acid (0.1 %) may be added to improve the resolution
  • the reaction mixture was diluted with EtOAc (500 mL) and aqueous hydrochloric acid (2 M, 500 mL) and the resulting suspension was filtered.
  • the filtrate was extracted with aqueous hydrochloric acid (2 M, 2 x 500 mL).
  • the combined aqueous extracts were basified with a saturated aq solution of sodium carbonate to pH 10.
  • the precipitate formed was filtered and the filtrate was extracted with EtOAc (3 x 1 L).
  • the combined organic extracts were dried, filtered and the solvent removed in vacuo to afford a grey solid.
  • the mixture was cooled to RT, diluted with EtOAc (3 mL) and washed with water (1 mL).
  • the aq phase was back extracted with EtOAc (1 .0 mL) and the combined organic extracts were washed with water (1 .0 mL).
  • the aq phase was back extracted with EtOAc (1 .0 mL) and the combined organic extracts were washed with water (1 .0 mL).
  • the aq phase was back extracted with EtOAc (1 .0 mL) and the combined organic extracts were washed with a saturated solution of aq ammonium chloride (2 x 2.0 mL) and then dried, filtered and evaporated in vacuo.
  • Example 1 (70 mg, 85%) as a white solid: m/z 534/536 (M+H) + (ES + ); R l 3.73 min; 1 H NMR (500 MHz, DMSO-d 6 ) ⁇ : 4.42 (1 H, s), 5.30 (2H, s), 5.77 (2H, s), 6.16 (1 H, d), 6.79 (1 H, t), 6.84 (1 H, dd), 6.89-6.96 (2H, m), 7.04 (1 H, t), 7.14 (1 H, d), 7.30 (1 H, t), 7.70 (1 H, d), 7.75 (1 H, d), 7.80-7.87 (1 H, m), 8.18 (1 H, s), 9.67 (1 H, s).
  • Example 2 2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-d]pyrimidin-1 -yl)methyl)-3- (2-chlorobenzyl)-5-(3-(2-(2-methoxyethoxy)ethoxy)prop-1 -yn-1 -yl)quinazolin-4(3H)-one.
  • Intermediate A 2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-d]pyrimidin-1 -yl)methyl)-3- (2-chlorobenzyl)-5-(3-(2-(2-methoxyethoxy)ethoxy)prop-1 -yn-1 -yl)quinazolin-4(3H)-one.
  • Example 3 2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-d]pyrimidin-1 -yl)methyl)-3- (2-chlorobenzyl)-5-(6-morpholino-6-oxohex-1 -yn-1 -yl)quinazolin-4(3H)-one.
  • Example 4 6-(2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-d]pyrimidin-1 -yl) methyl)-3-(2-chlorobenzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)hex-5-ynoic acid.
  • Example 5 2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-d]pyrimidin-1 -yl)methyl)-3- (2-chlorobenzyl)-5-(6-morpholino-6-oxohex-1 -yn-1 -yl)quinazolin-4(3H)-one.
  • Example 4 2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-d]pyrimidin-1 -yl)methyl)-3- (2-chlorobenzyl)-5-(6-morpholino-6-oxohex-1 -yn-1 -yl)quinazolin-4(3H)-one.
  • Example 4 To a solution of Example 4 (16.0 mg, 26 ⁇ ), 4-dimethylaminopyridine (0.6 mg, 5.2 ⁇ ), and EDC hydrochloride (5.3 mg, 28 ⁇ ) in DMF (0.5 mL) was added morpholine (2.3 ⁇ L, 26 ⁇ ). The reaction mixture was stirred at RT overnight and then diluted with water (5.0 mL).
  • Example 6 3-((2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-cf]pyrimidin-1 methyl)-5-(3-(2-(2-hydroxyethoxy)ethoxy)prop-1 -yn-1 -yl)-4-oxoquinazolin-3(4H)- yl)methyl)benzonitrile.
  • Example 7 2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-d]pyrimidin-1 -yl) methyl)-3-(2-chlorobenzyl)-5-(5-(cyclopentylamino)pent-1 -ynyl)quinazolin-4(3H)-one.
  • Example 7 was purified by flash column chromatography (Si0 2 , 12 g, MeOH in EtOAc, 0-100 %, gradient elution) and the crude product so obtained was triturated with MeCN to afford Example 7 as a yellow solid (16 mg, 17%); R l 1.39 min; m/z 659/661 (M+H) + (ES + ) (Method D); 1 H NMR (400 MHz, DMSO-de) ⁇ : 1 .22 (2H, m), 1.38 (2H, m), 1.50-1 .64 (6H, overlapping m), 2.46 (2H, t), 2.60 (2H, t), 2.89 (1 H, quin), 5.29 (2H, s), 5.73 (2H, s), 6.15 (1 H, d), 6.77 (1 H, td), 6.87 (2H, d), 7.02 (1 H, m), 7.1 1 (1 H, dd), 7.32 (2H, d), 7.58 (1 H, dd), 7.67
  • chiral stationary phase HPLC analysis of PIK294 indicates that the compound exists as readily separable, non-superimposable stereoisomers, that is as a 1 :1 mixture of enantiomeric atropisomers (Fig. 1 ). This assigment was confirmed by the separation of a sample of PIK294, using chiral stationary phase preparative HPLC, into its discrete atropisomers (Fig. 2).
  • PI3 kinases catalyse the phosphorylation of phosphatidylinositol 4,5-biphosphate (PIP2) to phosphatidylinositol 3,4,5-triphosphate (PIP3) in the presence of ATP and Mg 2+ ions.
  • the PIP3 product can be detected by displacement of biotin-PIP3 from energy transfer complexes consisting of europium labelled anti-GST monoclonal antibody, a GST-tagged Pleckstrin homology (PH) domain, biotinylated PIP3 and streptavidin-allophycocyanin (APC) by the time- resolved fluorescence resonance energy transfer (TR-FRET) (HTRFOPI3K enzyme assay, Millipore).
  • TR-FRET time- resolved fluorescence resonance energy transfer
  • Excitation (330 nm) of europium in the complex results in an energy transfer to the APC and a fluorescent emission at 665 nm although europium itself emits at its characteristic 620 nm.
  • the PIP3 product formed by PI3K activity displaces biotin-PIP3 from the complex and results in a loss of energy transfer (decreasing signal).
  • the compound to be tested was added, at the desired final concentrations, to a mixture of PIP2 substrate and recombinant PI3 kinase ⁇ , ⁇ or ⁇ enzymes (Millipore), and the mixture incubated for 2 hr at RT. Following this incubation period, ATP (20 ⁇ ) was added to the enzyme/compound/PIP2 substrate mixture and the resulting mixture was incubated for 30 min at RT.
  • a stopping solution containing biotinylated PIP3 and the detection mix containing the GST tagged GRP1 pleckstrin homology (PH) domain and fluorophores were then added and the mixture was incubated at RT for 15-18 hr, prior to detection in a fluorescence microplate reader (Varioskan® Flash, ThermoFisher Scientific).
  • PI3K5 cell based assay As a means of assessing PI3K ⁇ activation in response to stimuli, the phosphorylation status of the protein, Akt, a downstream product of PI3K5, signaling was determined.
  • Human monocytic cells (U937 cells), were differentiated to macrophage-type cells by incubation with phorbol myristate acetate (PMA; 100 ng/mL) for 48 to 72 hr. Cells were then pre-incubated with either the test compound or vehicle for 2 hr and were then stimulated briefly by exposure to H 2 0 2 (10 mM; 5-7 min) and the reaction stopped by replacing the media with 4% formaldehyde solution. Endogenous peroxide activity and formaldehyde were inactivated by incubating with quenching buffer (0.1 % sodium azide, 1 % H 2 0 2 in PBS with 0.1 % Triton X-100) for 20 min.
  • quenching buffer 0.1 % sodium azide, 1 % H 2 0 2 in PBS with 0.1 % Triton X-100
  • the cells were washed with buffer (PBS containing 0.1 % Triton X-100) and were incubated with blocking solution (1 % BSA in PBS) for 1 hr and were then re-washed with buffer and incubated overnight with either anti-pAkt antibody or anti-pan-Akt antibody (both from Cell Signaling Technology). After washing with buffer (PBS containing 0.1 % Triton X-100), cells were incubated with an HRP-conjugated secondary antibody (Dako) and the resultant signal was determined colorimetrically (OD: 450 nm with a reference wavelength of 655 nm) using TMB substrate (substrate reagent pack supplied by R&D Systems, Inc.).
  • This reaction was stopped by addition of 100 ⁇ _ of 1 N H 2 S0 4 solution. Cells were then washed with buffer (PBS containing 0.1 % Triton X-100) and 100 ⁇ _ of 5% crystal violet solution was applied for 30 min. After washing with buffer (PBS containing 0.1 % Triton X-100) 100 ⁇ _ of 1 % SDS was added to each well and the plates were shaken lightly for 1 hr prior to measuring the absorbance at 595 nm (Varioskan® Flash, Thermo-Fisher Scientific). The measured OD 450 -655 readings were corrected for cell number by dividing the OD 450 -655 by the OD 595 readings.
  • the ratio of pAkt signal to total Akt signal was used to quantitate the extent of PI3K ⁇ activation.
  • the percentage inhibition for each well was calculated relative to a 10 ⁇ g mL standard control (LY294002) set to 100% inhibition versus H 2 0 2 -only controls as 0% inhibition.
  • the IC 50 values were calculated from the concentration-response curves generated by the serial dilutions of the test compounds (Table 3 and Table 4).
  • mice determination of effect and duration of action
  • Non-fasted mice were dosed by the intra-tracheal route with either vehicle, or the test substance at the time points ("pre-dose") indicated with respect to the start of LPS treatment.
  • T 0, mice were placed into an exposure chamber and exposed to LPS.
  • Eight hr after LPS challenge animals were anesthetized, the trachea cannulated and BALF extracted by infusing and withdrawing 1 mL of PBS into the lungs via a tracheal catheter. Total and differential white cell counts in the BALF samples were measured using a Neubaur haemocytometer.
  • Cytospin smears of the BALF samples were prepared by centrifugation at 200 rpm for 5 min at RT and stained using a DiffQuik stain system (Dade Behring). Cells were counted using oil immersion microscopy.
  • the results for the compound of Example 1 are shown in Fig. 4.
  • the upper panel reveals the effect on BAL neutrophil numbers following the administration of Example 1 when the compound was dosed 2 hr before LPS challenge.
  • Table 5 The effect of escalating doses of compound Example 4 and Example 5 on the inhibition of LPS induced neutophilia in mouse lung
  • Table 6 Comparison the activities of compounds Example 4 and Example 5 to inhibit an evoked (LPS) inflammatory response in mouse lung 2 and 8 hr after administration
  • mice Males, 5 weeks old were exposed to cigarette smoke (4% cigarette smoke, diluted with compressed air) for 30 min/day for 1 1 days using a Tobacco Smoke Inhalation Experiment System for small animals (Model SIS-CS; Sibata Scientific Technology, Tokyo, Japan). Test substances were given intra-nasally (35 ⁇ of solution in 50% DMSO/PBS) and therapeutically twice daily for 3 days after the final cigarette smoke exposure. Twelve hr after the last dosing, animals were anesthetized, the trachea cannulated and bronchoalveolar lavage fluid (BALF) was collected.
  • BALF bronchoalveolar lavage fluid
  • alveolar macrophages and neutrophils were determined by FACS analysis (EPICS ® ALTRA II, Beckman Coulter, Inc., Fullerton, CA, USA) using anti- mouse MOMA2 antibody (macrophage) or anti-mouse 7/4 antibody (neutrophil).
  • Example 1 The results for the compound of Example 1 are shown in Fig. 5 for neutrophils (upper panel) and for activated alveolar macrophages (lower panel).
  • the cigarette smoke model used for this study is reported to be a corticosteroid refractory system, [To, Y. et al., Am. J. Respir. Crit. Care Med., 2010, 182:897-904; Medicherla, S. et al., J. Pharmacol. Exp. Then 2008, 324:921 -9] and the data reveal that dexamethasone (0.3-10 mg/kg, p.o.) was inactive.
  • the results obtained for Example 1 demonstrate that the compound possesses anti-inflammatory activity when administered as a monotherapy.
  • Example 1 when Example 1 was co-administered with fluticasone propionate, at a dose which lacks effect as monotherapy, a marked enhancement of anti-inflammatory activtiy was detected. Data for cell numbers are shown as the mean ⁇ SEM.

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Abstract

The present invention relates to compounds of formula (I) and to compositions comprising the same and to the use of the compounds and their 10 compositions in treatment, for example in the treatment of inflammatory diseases, in particular respiratory inflammatory disease. The invention also extends to methods of making said compounds.

Description

Compounds
Field of the invention
The invention relates to compounds which are inhibitors of phosphoinositide 3-kinases, (PI3 kinases). In particular the invention relates to compounds that inhibit the PI3 kinase delta subtype and optionally, in addition, the gamma and alpha sub-types thereof, and their use in therapy, including in pharmaceutical combinations, especially in the treatment of inflammatory diseases, including inflammatory diseases of the lung, such as COPD and asthma. The disclosure also extends to methods of preparing said compounds and pharmaceutical compositions comprising same.
Background of the invention
Lipid kinases catalyse the phosphorylation of lipids to produce species involved in the regulation of a wide range of physiological processes, including cellular migration and adhesion. The PI3-kinases belong to this class of enzymes and are membrane associated proteins which catalyse the phosphorylation of lipids which are themselves associated with cell membranes. The PI3-kinase delta (δ) isozyme (PI3 kinase δ) is one of four isoforms of type I PI3 kinases responsible for generating various 3'-phosphorylated phosphoinositides, that mediate cellular signalling and have been implicated in a number of cellular processes such as inflammation, growth factor signalling, malignant transformation and immunity (See Review by Rameh, L. E. and Cantley, L. C. J. Biol. Chem., 1999, 274:8347-8350).
Involvement of PI3 kinases in controlling inflammation has been confirmed in several models using pan-PI3 kinase inhibitors, such as LY-294002 and wortmannin (Ito, K. et al., J Pharmacol. Exp. Ther., 2007, 321 :1 -8). Recent studies have been conducted using either selective PI3 kinase inhibitors or in knock-out mice lacking a specific enzyme isoform. These studies have demonstrated the role of the pathways controlled by PI3 kinase enzymes in inflammation. The PI3 kinase δ selective inhibitor IC-87114 was found to inhibit airway hyper- responsiveness, IgE release, pro-inflammatory cytokine expression, inflammatory cell accumulation into the lung and vascular permeability in ovalbumin-sensitized, ovalbumin- challenged mice [Lee, K. S. et al., J. Allergy Clin. Immunol., 2006, 1 18:403-409 and Lee, K. S. et al., FASEB J., 2006, 20:455-65]. In addition, IC-87114 lowered neutrophil accumulation in the lungs of mice and neutrophil function, stimulated by TNFa [Sadhu, C. et al., Biochem. Biophys. Res. Commun., 2003, 308:764-9]. The PI3 kinase δ isoform is activated by insulin and other growth factors, as well as G-protein coupled protein signaling and inflammatory cytokines. Recently the PI3 kinase dual δ/γ inhibitor TG100-115 was reported to inhibit pulmonary eosinophilia, interleukin-13 as well as mucin accumulation and airways hyperesponsiveness in a murine model, when administered by aerosolisation. The same authors also reported that the compound was able to inhibit pulmonary neutrophilia elicited by either LPS or cigarette smoke [Doukas, J. et al., J Pharmacol. Exp. Ther., 2009, 328.758-765] Since it is also activated by oxidative stress, the PI3 kinase δ isoform is likely to be relevant as a target for therapeutic intervention in those diseases where a high level of oxidative stress is implicated. Downstream mediators of the PI3 kinase signal transduction pathway include Akt (a serine/threonine protein kinase) and the mammalian target of rapamycin, the enzyme rmTOR. Recent work has suggested that activation of PI3 kinase δ, leading to phosphorylation of Akt, is able to induce a state of corticosteroid resistance in otherwise corticosteroid-sensitive cells [To, Y. et al., Am. J. Respir. Crit. Care Med., 2010, 182:897-904]. These observations have led to the hypothesis that this signalling cascade could be one mechanism responsible for the corticosteroid-insensitivity of inflammation observed in the lungs of patients suffering from COPD, as well as those asthmatics who smoke, thereby subjecting their lungs to increased oxidative stress. Indeed, theophylline, a compound used in the treatment of both COPD and asthma, has been suggested to reverse steroid insensitivity through mechanisms involving interaction with pathways controlled by PI3 kinase δ [To, Y. et al., Am. J. Respir. Crit. Care Med., 2010, 182:897-904].
At present the mainstay of treatment for both asthma and COPD is inhaled therapy, using a combination of corticosteroids, muscarinic antagonists and 32-agonists, as judged clinically appropriate. One way of addressing the unmet medical needs in COPD and asthma is to identify new therapeutic agents, for example suitable for use as inhaled medicines, which have the potential to provide significant benefit when used as a monotherapy or in combination with one or more medicaments from these three pharmacological classes. Therefore, there remains a need to identify and develop isoform selective PI3 kinase inhibitors which have the potential to provide enhanced therapeutic efficacy in asthma, COPD and other inflammatory diseases. Summary of the invention
According to the invention, there is formula (I):
Figure imgf000004_0001
wherein
is H, a saturated or unsaturated, branched or unbranched CMS alkyl chain, wherein optionally one or more carbons (for example 1 , 2 or 3 carbons, suitably 1 or 2, in particular 1 ) is/are replaced by a heteroatom selected from O, N, S(0)p, (for example a CH2, group is replaced with O, or with NH, or with S, or with S02 or a -CH3 group at the terminus of the chain or on a branch is replaced with OH or with NH2) wherein said chain is optionally substituted by one or more groups (for example 1 to 3, such as 2 groups) independently selected from oxo, halogen, an aryl group, a heteroaryl group, a carbocyclyl group or a heterocyclyl group, each aryl, heteroaryl, carbocyclyl or heterocyclyl group bearing:
0 to 3 substituents selected from halogen, -hydroxyl, -Ci-6 alkyl, -Ci-6 alkoxy, -C2-3 alkoxyOCi-3 alkyl, -C2-3 alkylOCi-3 alkyl, -Ci-3 hydroxyalkyl, -Ci-6 haloalkyl, amino, -Ci-4 mono or -C2-8 di-alkyl amino, -Ci-4 mono or -C2-8 di-acyl amino, -Co-6 alkylS(0)pCi-6 alkyl, -C0-6 alkylS(0)pNR6R7, -C0-6 alkylNR8Co-6alkylS(0)pCi-6 alkyl, -C0-6 alkylC(O)OC0-6 alkyl, -NR8C0-6 alkylC(0)NR6R7 -NR8C0-6 alkylC(O)C0-6 alkyl, -C0-6 alkylC(0)NR6R7, and -C0-6 alkylC(0)Ci-6 alkyl; and/or one aryl, heterocyclyl or carbocyclyl; is C6-io aryl or a C5-9 heteroaryl each substituted by R and by R'
wherein R2a is selected from hydrogen, -Ci-3 alkyl, halo, hydroxyl, cyano, -Ci-3 haloalkyl, -Ci-3 alkoxy, -C2-3 alkoxyOCi-3 alkyl, -C2-3 alkylOCi-3 alkyl, -Ci-3 hydroxyalkyl, -C0-6 alkylS(0)qCi-3 alkyl, -C0-6 alkylS(0)pNR6R7, -C0-6 alkylNR8C0- 6alkylS(0)pCi-6 alkyl, -C0-6 alkylC(0)OH, -C0-6 alkylC(0)OCi-6 alkyl, -NR8C0-6 alkylC(0)NR6R7, -NR8C0-6 alkylC(0)Ci-6 alkyl, -C0-6 alkylC(0)NR6R7 and -Co-6 alkylC(0)Ci-6 alkyl; and
R2b is selected from hydrogen, Ci-3 alkyl, halo, hydroxyl, cyano, -Ci-3 haloalkyl, -Ci-3 alkoxy, and -C0-6 alkylS(0)qCi-3 alkyl;
R is hydroxyl;
R3b is selected from hydrogen, hydroxyl, halo, cyano, -Ci-3 haloalkyl, -Ci-3 hydroxyalkyl, -Ci-3 alkoxy, and -S(0)qCi-3 alkyl;
R4 is hydrogen or -Ci-3 alkyl;
R5 is hydrogen or -Ci-3 alkyl;
R6 is hydrogen or -Ci-6 alkyl;
R7 is hydrogen or -Ci-6 alkyl;
R8 is hydrogen or -Ci-6 alkyl;
p is 0 or an integer 1 or 2;
q is 0 or an integer 1 or 2
or a pharmaceutically acceptable salt thereof, including all stereoisomers, tautomers and isotopic derivatives thereof.
In one embodiment R1 is H, a saturated or unsaturated, branched or unbranched
Figure imgf000005_0001
alkyl chain, wherein optionally one or more carbons (for example 1 , 2 or 3 carbons, suitably 1 or 2, in particular 1 ) is/are replaced by a heteroatom selected from O, N, S(0)p, (for example a CH2, group is replaced with O, or with NH, or with S, or with S02 or a -CH3 group at the terminus of the chain or on a branch is replaced with OH or with NH2) wherein said chain is optionally substituted by one or more groups (for example 1 to 3, such as 2 groups) independently selected from oxo, halogen, an aryl group, a heteroaryl group, a carbocyclyl group or a heterocyclyl group, each aryl, heteroaryl, carbocyclyl or heterocyclyl group bearing:
0 to 3 substituents selected from halogen, hydroxyl, -Ci-6 alkyl, -Ci-6 alkoxy, -C2-3 alkylOCi-3 alkyl, -C2-3 alkoxyOCi-3 alkyl, -Ci-3 hydroxyalkyl, -Ci-6 haloalkyl, amino, -Ci-4 mono or -C2-8 di-alkyl amino, -Ci-4 mono or -C2-8 di-acyl amino, -Co-6 alkylS(0)pCi-6 alkyl,-C0-6 alkylS(0)pNR6R7, -C0-6 alkylNR8Co-6alkylS(0)pCi-6 alkyl, -C0-6 alkylC(O)OC0-6 alkyl, -NR8C0-6 alkylC(0)NR6R7 -NR8C0-6 alkylC(O)C0-6 alkyl, -C0-6 alkylC(0)NR6R7, and -C0-6 alkylC(0)Ci-6 alkyl; and/or one aryl, heterocyclyl or carbocyclyl. one embodiment there is provided a compound of formula (I)
Figure imgf000006_0001
wherein
R1 is H, a saturated or unsaturated, branched or unbranched CMO alkyl chain, wherein optionally one or more carbons (for example 1 , 2 or 3 carbons, suitably 1 or 2, in particular 1 ) is/are replaced by a heteroatom selected from O, N, S(0)p, (for example a
CH2, group is replaced with O, or with NH, or with S, or with S02 or a -CH3 group at the terminus of the chain or on a branch is replaced with OH or with NH2) wherein said chain is optionally substituted by one or more groups (for example 1 to 3, such as 2 groups) independently selected from oxo, halogen, an aryl group, a heteroaryl group, a carbocyclyl group or a heterocyclyl group, each aryl, heteroaryl, carbocyclyl or heterocyclyl group bearing:
0 to 3 substituents selected from halogen, -Ci-6 alkyl, -Ci-6 alkoxy, -C2-3 alkoxyOCi-3 alkyl, -Ci-6 haloalkyl, amino, -Ci-4 mono or -C2-8 di-alkyl amino, -Ci-4 mono or -C2-8 di-acyl amino, -C0-6 alkylS(0)pCi-6 alkyl, -C0-6 alkylS(0)pNR6R7, -C0-6 alkylNR8Co-6alkylS(0)pCi-6 alkyl, -C0-6 alkylC(O)OC0-6 alkyl, -NC0-6 alkylC(0)NR6R7 -NC0-6 alkylC(O)C0-6 alkyl, -C0-6 alkylC(0)NR6R7, and -Co-6 alkylC(0)Ci-6 alkyl; and/or one aryl, heterocyclyl or carbocyclyl; X is C6-io aryl or a C5-9 heteroaryl each substituted by R and optionally by R wherein R2a is hydrogen, -Ci-3 alkyl, halo, hydroxyl, cyano, -Ci-3 haloalkyl, -Ci-3 alkoxy, - C2-3 alkoxyOCi-3 alkyl, -Ci-3 hydroxyalkyl, -C0-6 alkylS(0)qCi-3 alkyl, -C0-6 alkylS(0)pNR6R7, -C0-6 alkylNR8Co-6alkylS(0)pCi-6 alkyl, -C0-6 alkylC(0)OH, -C0-6 alkylC(0)OCi-6 alkyl, -NC0-6 alkylC(0)NR6R7, -NR8C0-6 alkylC(0)Ci-6 alkyl, -C0-6 alkylC(0)NR6R7 and -C0-6 alkylC(0)Ci-6 alkyl; and
R2b is hydrogen, Ci-3 alkyl, halo, cyano, -Ci-3 haloalkyl, -Ci-3 alkoxy, -C0-6 alkylS(0)qCi-3 alkyl;
R3a is hydroxyl;
R3b is hydrogen, hydroxyl, halo, cyano, -Ci-3 haloalkyl, -Ci-3 hydroxyalkyl, -Ci-3 alkoxy, -S(0)qCi-3 alkyl;
R4 is hydrogen or -Ci-3 alkyl;
R5 is hydrogen or -Ci-3 alkyl;
R6 is hydrogen or -Ci-6 alkyl;
R7 is hydrogen or -Ci-6 alkyl;
R8 is hydrogen or -Ci6 alkyl;
P is 0 or an integer 1 or 2;
q is 0 or an integer 1 or 2
or a pharmaceutically acceptable salt thereof, including all stereoisomers, tautomers and isotopic derivatives thereof.
In one embodiment the present disclosure provides a compound of formula (ΙΑ'):
Figure imgf000007_0001
is H , a saturated or unsaturated, branched or unbranched CMS alkyl chain, wherein optionally one or more carbons (for example 1 , 2 or 3 carbons, suitably 1 or 2, in particular 1 ) is/are replaced by a heteroatom selected from O, N , S(0)p, (for example a CH2, group is replaced with O, or with N H , or with S, or with S02 or a -CH3 group at the terminus of the chain or on a branch is replaced with OH or with N H2) wherein said chain is optionally substituted by one or more groups (for example 1 to 3, such as 2 groups) independently selected from oxo, halogen, an aryl group, a heteroaryl group, a carbocyclyl group or a heterocyclyl group, each aryl, heteroaryl, carbocyclyl or heterocyclyl group bearing:
0 to 3 substituents selected from halogen, -hydroxyl, -Ci-6 alkyl, -Ci-6 alkoxy,
-C2-3 alkoxyOCi-3 alkyl, -C2-3 alkylOCi-3 alkyl, -Ci-3 hydroxyalkyl, -Ci-6 haloalkyl, amino, -Ci-4 mono or -C2-8 di-alkyl amino, -Ci-4 mono or -C2-8 di-acyl amino, -C0-6 alkylS(0)pCi-6 alkyl, -C0-6 alkylS(0)pNR6R7, -C0-6 alkylNR8Co-6alkylS(0)pCi-6 alkyl, - Co-6 alkylC(O)OC0-6 alkyl, -NR8C0-6 alkylC(0)NR6R7 -NR8C0-6 alkylC(O)C0-6 alkyl, -C0- 6 alkylC(0)NR6R7, and -C0-6 alkylC(0)Ci-6 alkyl; and/or one aryl, heterocyclyl or carbocyclyl;
R2a is hydrogen, -Ci-3 alkyl, halo, hydroxyl, cyano, -Ci-3 haloalkyl, -Ci-3 alkoxy, -C2-3 alkoxyOCi-3 alkyl, -Ci-3 hydroxyalkyl, -C0-6 alkylS(0)qCi-3 alkyl, -C0-6 alkylS(0)pNR6R7, -C0-6 alkylNR8C0-6alkylS(O)pCi-6 alkyl, -C0-6 alkylC(0)OH, -C0-6 alkylC(0)OCi-6 alkyl, -NC0-6 alkylC(0)NR6R7, -NR8C0-6 alkylC(0)d-6 alkyl, -C0-6 alkylC(0)NR6R7 and -C0-6 alkylC(0)Ci-6 alkyl;
R2b is hydrogen, Ci-3 alkyl, halo, cyano, Ci-3 haloalkyl, Ci-3 alkoxy, C0-6 alkylS(0)qCi-3 alkyl; R3a is hydroxyl;
R3b is hydrogen, hydroxyl, halo, cyano, Ci-3 haloalkyl, Ci-3 hydroxyalkyl, Ci-3 alkoxy, S(0)qCi-3 alkyl;
R4 is hydrogen or -Ci-3 alkyl
R5 is hydrogen or -Ci-3 alkyl
R6 is hydrogen or -Ci-6 alkyl
R7 is hydrogen or -Ci-6 alkyl
R8 is hydrogen or -Ci6 alkyl;
P is 0 or an integer 1 or 2;
q is 0 or an integer 1 or 2
or a pharmaceutically acceptable salt thereof, including all stereoisomers, tautomers and isotopic derivatives thereof.
In one embodiment the present disclosure provides a compound of formula (IA):
Figure imgf000008_0001
R1 is H , a saturated or unsaturated, branched or unbranched CMS alkyl chain (such as a C-i-12 alkyl chain or a CMO alkyl chain), wherein optionally one or more carbons (for example 1 , 2 or 3 carbons, suitably 1 or 2, in particular 1 ) is/are replaced by a heteroatom selected from O, N , S(0)p, (for example a CH2, group is replaced with O, or with N H , or with S, or with S02 or a -CH3 group at the terminus of the chain or on a branch is replaced with OH or with N H2) wherein said chain is optionally substituted by one or more groups independently selected from oxo, halogen, an aryl group, a heteroaryl group, a carbocyclyl group or a heterocyclyl group each aryl, heteroaryl, carbocyclyl or heterocyclyl group bearing:
0 to 3 substituents selected from halogen, -Ci-6 alkyl, -Ci-6 alkoxy, -C2-3 alkoxyOCi-3 alkyl, -Ci-6 haloalkyl, amino, -Ci-4 mono or -C2-8 di-alkyl amino, -Ci-4 mono or -C2-8 di-acyl amino, -C0-6 alkylS(0)pCi-6 alkyl, -C0-6 alkylS(0)pNR6R7, -C0-6 alkylNR8Co-6alkylS(0)pCi-6 alkyl, -C0-6 alkylC(O)OC0-6 alkyl, -NC0-6 alkylC(0)NR6R7 -NC0-6 alkylC(O)C0-6 alkyl, -C0-6 alkylC(0)NR6R7, and -Co-6 alkylC(0)Ci-6 alkyl; and/or one aryl, heterocyclyl or carbocyclyl;
R is hydrogen, -Ci-3 alkyl, halo, hydroxyl, cyano, -Ci-3 haloalkyl, -Ci-3 alkoxy, -C2-3 alkoxyOCi-3 alkyl, -Ci-3 hydroxyalkyl, -C0-6 alkylS(0)qCi-3 alkyl, -C0-6 alkylS(0)pNR6R7, -C0-6 alkylNR8C0-6alkylS(O)pCi-6 alkyl, -C0-6 alkylC(0)OH, -C0-6 alkylC(0)OCi-6 alkyl, -NC0-6 alkylC(0)NR6R7, -NR8C0-6 alkylC(0)Ci-6 alkyl, -C0-6 alkylC(0)NR6R7 and -C0-6 alkylC(0)Ci-6 alkyl;
R2b is hydrogen, Ci-3 alkyl, halo, cyano, Ci-3 haloalkyl, Ci-3 alkoxy, C0-6 alkylS(0)qCi-3 alkyl; R3a is hydroxyl;
R3b is hydrogen, hydroxyl, halo, cyano, Ci-3 haloalkyl, Ci-3 hydroxyalkyl, Ci-3 alkoxy, S(0)qCi-3 alkyl;
R4 is hydrogen or -Ci-3 alkyl;
R5 is hydrogen or -Ci-3 alkyl;
R6 is hydrogen or -Ci-6 alkyl;
R7 is hydrogen or -Ci-6 alkyl;
R8 is hydrogen or -Ci6 alkyl;
p is 0 or an integer 1 or 2;
q is 0 or an integer 1 or 2
or a pharmaceutically acceptable salt thereof, including all stereoisomers, tautomers and isotopic derivatives thereof. According to the invention, there is provided a compound of formula (IB'):
Figure imgf000010_0001
wherein is H, a saturated or unsaturated, branched or unbranched CMS alkyl chain, wherein optionally one or more carbons (for example 1 , 2 or 3 carbons, suitably 1 or 2, in particular 1 ) is/are replaced by a heteroatom selected from O, N , S(0)P, (for example a CH2, group is replaced with O, or with NH , or with S, or with S02 or a -CH3 group at the terminus of the chain or on a branch is replaced with OH or with NH2) wherein said chain is optionally substituted by one or more groups (for example 1 to 3, such as 2 groups) independently selected from oxo, halogen, an aryl group, a heteroaryl group, a carbocyclyl group or a heterocyclyl group, each aryl, heteroaryl, carbocyclyl or heterocyclyl group bearing:
0 to 3 substituents selected from halogen, -hydroxyl, -Ci-6 alkyl, -Ci-6 alkoxy, -C2-3 alkoxyOCi-3 alkyl, -C2-3 alkylOCi-3 alkyl, -Ci-3 hydroxyalkyl, -Ci-6 haloalkyl, amino, -Ci-4 mono or -C2-8 di-alkyl amino, -Ci-4 mono or -C2-8 di-acyl amino, -Co-6 alkylS(0)pCi-6 alkyl, -C0-6 alkylS(0)pNR6R7, -C0-6 alkylNR8Co-6alkylS(0)pCi-6 alkyl, -C0-6 alkylC(O)OC0-6 alkyl, -NR8C0-6 alkylC(0)NR6R7 -NR8C0-6 alkylC(O)C0-6 alkyl, -C0-6 alkylC(0)NR6R7, and -C0-6 alkylC(0)d-6 alkyl; and/or one aryl, heterocyclyl or carbocyclyl;
R2a is hydrogen, -Ci-3 alkyl, halo, hydroxyl, cyano, -Ci-3 haloalkyl, -Ci-3 alkoxy, -C2-3 alkoxyOCi-3 alkyl, -Ci-3 hydroxyalkyl, -C0-6 alkylS(0)qCi-3 alkyl, -C0-6 alkylS(0)pNR6R7, -C0-6 alkylNR8C0-6alkylS(O)pCi-6 alkyl, -C0-6 alkylC(0)OH, -C0-6 alkylC(0)OCi-6 alkyl, -NC0-6 alkylC(0)NR6R7, -NR8C0-6 alkylC(0)Ci-6 alkyl, -C0-6 alkylC(0)NR6R7 and -C0-6 alkylC(0)Ci-6 alkyl;
R3a is hydroxyl;
R4 is hydrogen or -Ci-3 alkyl;
R5 is hydrogen or-Ci-3 alkyl
R6 is hydrogen or -Ci-6 alkyl
R7 is hydrogen or -Ci-6 alkyl
R8 is hydrogen or -Ci6 alkyl; p is 0 or an integer 1 or 2;
q is 0 or an integer 1 or 2
or a pharmaceutically acceptable salt thereof, including all stereoisomers, tautomers and isotopic derivatives thereof.
According to the invention, there is provided a compound of formula (IB):
Figure imgf000011_0001
wherein
R1 is H, a saturated or unsaturated, branched or unbranched CMS alkyl chain (such as a C-i-12 alkyl chain or a CMO alkyl chain), wherein optionally one or more carbons (for example 1 , 2 or 3 carbons, suitably 1 or 2, in particular 1 ) is/are replaced by a heteroatom selected from O, N, S(0)p, (for example a CH2, group is replaced with O, or with NH, or with S, or with S02 or a -CH3 group at the terminus of the chain or on a branch is replaced with OH or with NH2) wherein said chain is optionally substituted by one or more groups independently selected from oxo, halogen, an aryl group, a heteroaryl group, a carbocyclyl group or a heterocyclyl group each aryl, heteroaryl, carbocyclyl or heterocyclyl group bearing:
0 to 3 substituents selected from halogen, -Ci-6 alkyl, -Ci-6 alkoxy, -C2-3 alkoxyOCi-3 alkyl, -Ci-6 haloalkyl, amino, -Ci-4 mono or -C2-8 di-alkyl amino, -Ci-4 mono or -C2-8 di-acyl amino, -C0-6 alkylS(0)pCi-6 alkyl, -C0-6 alkylS(0)pNR6R7, -C0- 6 alkylNR8C0-6alkylS(O)pCi-6 alkyl, -C0-6 alkylC(O)OC0-6 alkyl, -NC0-6 alkylC(0)NR6R7 -NC0-6 alkylC(O)C0-6 alkyl, -C0-6 alkylC(0)NR6R7, and -C0-6 alkylC(0)Ci-6 alkyl; and/or one aryl, heterocyclyl or carbocyclyl;
R2a is hydrogen, -Ci-3 alkyl, halo, hydroxyl, cyano, -Ci-3 haloalkyl, -Ci-3 alkoxy, -C2-3 alkoxyOCi-3 alkyl, -Ci-3 hydroxyalkyl, -C0-6 alkylS(0)qCi-3 alkyl, -C0-6 alkylS(0)pNR6R7, -C0-6 alkylNR8C0-6alkylS(O)pCi-6 alkyl, -C0-6 alkylC(0)OH, -C0-6 alkylC(0)OCi-6 alkyl, -NC0-6 alkylC(0)NR6R7, -NR8C0-6 alkylC(0)Ci-6 alkyl, -C0-6 alkylC(0)NR6R7 and -C0-6 alkylC(0)Ci-6 alkyl;
R3a is hydroxyl;
R4 is hydrogen or -Ci-3 alkyl; R5 is hydrogen or -Ci-3 alkyl;
R6 is hydrogen or -Ci-6 alkyl;
R7 is hydrogen or -Ci-6 alkyl;
R8 is hydrogen or -Ci6 alkyl;
p is 0 or an integer 1 or 2;
q is 0 or an integer 1 or 2
or a pharmaceutically acceptable salt thereof, including all stereoisomers, tautomers and isotopic derivatives thereof.
In one embodiment there is provided a compound of formula (IC):
Figure imgf000012_0001
wherein R1, R3a, R3b, R4 and R5 are as defined above for compounds of formula (I). In one embodiment there is provided a compound of formula (ID):
Figure imgf000012_0002
wherein R1, R2a, R2b, R3a, R3b, R4 and R5 are as defined above for compounds of formula (I). In one embodiment there is provided a compound of formula (IE):
Figure imgf000013_0001
wherein R1, R3a, R3b, R4 and R5 are as defined above for compounds of formula (I). In one embodiment there is provided a compound of formula (IF):
Figure imgf000013_0002
wherein R1, R2a, R3a, R3b, R4 and R5 are as defined above for compounds of formula (I).
In one embodiment there is provided a compound of formula (IG):
Figure imgf000013_0003
wherein R1, R2a, R3a, R3b, R4 and R5 are as defined above for compounds of formula (I).
The compounds of the present disclosure are at least PI3K delta inhibitors. Certain compound of the disclosure may be PI3K alpha and delta inhibitors. Certain compounds of the disclosure may be PI3K delta and gamma inhibitors. Some compounds may be alpha and gamma isozyme inhibitors. Other compounds may be alpha, delta and gamma subtype inhibitors. It is proposed that different biological profiles may be associated with these different inhibition profiles which may be advantageous in targeting pathways for different diseases.
Inhibitor as employed here is intended to refer to a compound that reduces (for example by at least 50%) or eliminates the biological activity of the target, for example PI3K delta, in an in vitro enzyme assay. The compounds of the present disclosure are active in cell based screening systems and thereby demonstrate that they possess suitable properties for penetrating cells.
General references to compounds of formula (I) herein are intended to include references to compounds of formula (IA) - (IG) unless the context suggests otherwise.
Alkyl as used herein refers to straight chain or branched chain alkyl, such as, without limitation, methyl, ethyl, propyl, /so-propyl, butyl, and ie f-butyl. In one embodiment alkyl refers to straight chain alkyl. Alkoxy as used herein refers to straight or branched chain alkoxy, for example methoxy, ethoxy, propoxy, butoxy. Alkoxy as employed herein also extends to embodiments in which the oxygen atom is located within the alkyl chain, for example -(CH2)nOCH3. In one embodiment the alkoxy is linked through oxygen to the remainder of the molecule. In one embodiment the alkoxy is linked through carbon to the remainder of the molecule. In one embodiment the disclosure relates to straight chain alkoxy.
-C2-3 alkoxyOCi-3 alkyl as employed herein is intended to refer an -C2-3 alkoxy linked to the remaider of the molecule through oxygen to provide oxygen-alkylene-oxygen-alkyl. Carbocyclyl as employed herein is intended to refer to C3-10 saturated or partially saturated carbocyclic ring systems, for example cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl.
Heteroaryl is a C5-9 membered aromatic carbocylic ring or bicyclic ring system comprising one or more, (for example 1 , 2, 3 or 4) heteroatoms independently selected from O, N and S. Examples of heteroaryls include: pyrrole, oxazole, thiazole, isothiazole, imidazole, pyrazole, isoxazole, pyridazine, pyrimidine, pyrazine, benzothiophene, benzofuran, or 1 , 2, 3 and 1 , 2, 4 triazole. In a bicyclic ring system the definition of heteroaryl will be satisfied if at least one ring contains a heteroatom and at least one ring is aromatic. The heteroaryl may be linked to the remainder of the molecule through a carbocyclic ring or a ring comprising a heteroatom.
In a bicyclic system the definition of aromatic will be satisfied by the aromatic nature of at least one ring in the system.
Heterocyclic groups as employed herein is intended to refer to a 5 to 10 membered ring system which is saturated or partially unsaturated and which is non-aromatic comprising one or more (for example 1 , 2 or 3 in particular 1 or 2,) heteroatoms independently selected from O, N and S, for example 5 or 6 or 7 membered rings including pyrrolidine, tetrahydrofuran, tetrahydrothiophene, piperidine, piperazine, morpholine, 1 ,4-dioxane, pyrrolidine and oxoimidazolidine such as pyrrolidine, tetrahydrofuran, tetrahydrothiophene, piperidine, piperazine, morpholine, and 1 ,4-dioxane, and in particular piperidine, piperazine, and morpholine. An example of a saturated 7 membered heterocyclic ring is a 1 ,4-diazepane ring and an example of a partially unsaturated 7 membered ring is a 2,3,4,5-tetrahydro-1 ,4- oxazepine.
A heterocyclic group may be linked to, for example, an alkyl chain, through carbon or a suitable heteroatom such as N.
Oxo as employed herein refers to the group =0 and can be attached to a cabon atom to give C=0 or to a heteroatom to give, for example, S=0 or S02.
Haloalkyi as employed herein is intended to refer to a group having from 1 to 6 halo atoms, for example 1 to 5 halo atoms. In one embodiment the haloalkyi is per haloalkyi, for example per fluoroalkyl, such as CF2CF3 and CF3.
Halo represents bromo, chloro or fluoro. Ci-4 mono or C2-8 di acyl is intended to refer to -C(0)Ci-4 alkyl and -(COCi-4 alkyl)2 respectively.
Aryl as used herein refers to, for example C6-i4 mono or polycyclic groups (such as C6-io mono or bicyclic groups) having from 1 to 3 rings wherein at least one ring is aromatic including phenyl, napthyl, anthracenyl, 5,6,7,8-tetrahydronapthyl and the like, such as phenyl and napthyl.
In one embodiment X is phenyl. In one embodiment X is a C5-9 membered heteroaryl.
In one embodiment X is pyrazinyl, such as pyrazin-2-yl, in particular methylpyrazin-2-yl, more specifically 5-methylpyrazin-2-yl. In one embodiment X is benzothiophenyl, for example benzothiophen-2-yl. In one embodiment X is benzofuranyl, for example benzofura-5-yl.
In one embodiment X is thiazolyl, for example thiazol-4-yl.
In one embodiment X is isoxazolyl, for example isoxazol-3-yl, such as 5-methylisoxazol-3-yl. In R1 a suitable heteroatom may replace any carbon including a linking carbon, branched carbon or a terminal carbon and hydrogen(s) may fill the remaining valencies, as appropriate.
In one embodiment R1 is H. In another embodiment R1 is branched or unbranched CMO alkyl chain, wherein at least one carbon (for example 1 , 2 or 3 carbons) is replaced by an oxygen, for example C9 alkyl comprising 1 , 2 or 3 oxygen atoms, such as -CH2OCH2CH2OCH2CH2OCH3.
In one embodiment R1 is -CH2OCH3.
In one embodiment R1 is -CH2CH2CH2OH. In one embodiment R1 is -CH2OCH2CH2OCH2CH2OH. In one embodiment R1 bears at least one oxo substituent. In one embodiment R1 is -CH2NHCONH2.
In one embodiment R1 bears at least one carbocyclic group as a substitutent.
In one embodiment R1 bears at least one oxo substituent and a carbocyclic group substituent, for example cyclopentyl or cyclohexyl linked to a carbon bearing an oxo substituent, such as the fragment:
Figure imgf000016_0001
one embodiment R1 comprises
Figure imgf000016_0002
In one embodiment R1 comprises:
Figure imgf000017_0001
In one embodiment R1 comprises:
Figure imgf000017_0002
In one embodiment R1 comprises:
Figure imgf000017_0003
In one embodiment R1 bears at least one heterocyclic group substituent.
In one embodiment R1 bears one oxo substituent and one heterocyclic group substituent, for example the heterocyclic group is linked to a carbon bearing an oxo substituent, such as R1 comprising -C(0)morpholinyl wherein the morpholinyl may be linked through N, in particular the fragment:
Figure imgf000017_0004
or
-C(0)piperidinyl such as the fragment
Figure imgf000017_0005
In one embodiment R1 bears one oxo substituent and one heterocyclic group substituent, for example the heterocyclic group is linked to a carbon bearing an oxo substituent, wherein said heterocyclic group itself bears a Ci-6 alkyl or a -C(0)Ci-6 alkyl substitutent, such as R1 comprising -C(0)piperidinylCi-6 alkyl or -C(0)piperidinylC(0)Ci-6 alkyl wherein the piperidinyl may be linked through N, in particular the fragments:
Figure imgf000017_0006
In one embodiment R1 represent
Figure imgf000018_0001
In one embodiment R1 represents:
Figure imgf000018_0002
In one embodiment R1 represents:
Figure imgf000018_0003
In one embodiment R1 bears one oxo substituent and one heterocyclic group substituent, for example the heterocyclic group is linked to a carbon bearing an oxo substituent, wherein said heterocyclic group itself bears a heterocyclic group as a substituent thereon, such as R1 comprising -C(0)piperidinylmorpholinyl wherein the morpholinyl may be linked through N, in particular the fragment:
Figure imgf000018_0004
In one embodiment R1 comprises:
Figure imgf000018_0005
In one embodiment R1 comprises:
Figure imgf000018_0006
In one embodiment R1 comprises:
Figure imgf000018_0007
In one embodiment R1 represents:
Figure imgf000019_0001
In one embodiment R1 represents:
Figure imgf000019_0002
In one embodiment R1 represents:
Figure imgf000019_0003
In one embodiment R1 represents:
Figure imgf000019_0004
In one embodiment R1 represents:
Figure imgf000019_0005
In one embodiment R1 represents:
Figure imgf000019_0006
In one embodiment R1 represents:
In one embodiment R1 represents:
Figure imgf000019_0007
In one embodiment R1 represents:
Figure imgf000019_0008
In one embodiment R1 represents:
Figure imgf000020_0001
In one embodiment R1 represents:
In one embodiment R1 represents:
Figure imgf000020_0002
In one embodiment R1 represents:
Figure imgf000020_0003
In one embodiment R1 comprises a carboxylic acid, for example Ci-3 alkyleneC02H such as the fragment -CH2CH2CH2CO2H or alternatively -CH2CH2CH2CH2CO2H.
In one embodiment R1 represents -CH2CH2CH2C(0)NHCH(CI-l3)2.
In one embodiment R1 represents -CH2CH2CH2C(0)(CH3)2.
In one embodiment R1 represents -CH2CH2CH2NHCH2CH2OCH3. In one embodiment R1 represents -CH2CH2CH2N(CH2CH2OCH3)2. In one embodiment R1 represents -CH2CH2CH2C(0)N(CH2CH2OCH3)2. In one embodiment R1 represents -CH2NHC(0)CH2NHC(0)CH3. In one embodiment R1 represents -CH2CH2CH2C(0)NHCH2CH2OCI-l3.
In one embodiment R1 comprises:
Figure imgf000020_0004
one embodiment R1 comprises
Figure imgf000020_0005
In one embodiment R1 represents:
Figure imgf000021_0001
In one embodiment R1 represents:
Figure imgf000021_0002
In one embodiment R is located in the ortho position. In one embodiment R2a is located in the meta position. In one embodiment R2a is located in the para position.
In one embodiment R2a is Ci-3alkyl, halo, cyano, Ci-3 haloalkyl, Ci-3 alkoxy, S(0)qCi-3 alkyl, -C(0)OCi-6 alkyl, -NC(0)NCi-6 alkyl, -NC(0)Ci-6 alkyl, -C(0)NCi-6 alkyl, and -C(0)Ci-6 alkyl.
In one embodiment R2a is selected from the group comprising methyl, chloro, fluoro, cyano, methoxy, trifluoromethyl and S02CH3. In one embodiment R2a is chloro.
In one embodiment R2a is fluoro.
In one embodiment R2a is cyano.
In one embodiment R2a is methoxy. In one embodiment R2a is methyl. In one embodiment R2a is S02CH3.
In one embodiment R2a is -C(0)OCH3. In one embodiment R2a is CF3.
In one embodiment R2b is hydrogen. In one embodiment R2b is chloro. In one embodiment R2b is -OCH3. In one embodiment R are R are in the 2,3 or 2,4 or 3,4, or 3,5 positions respectively. In one embodiment R2a is CF3 and R2b is methoxy.
R3a is suitably in the ortho, meta or para position, such as the ortho or para position. In one embodiment R3b is hydrogen. In one embodiment R3b is fluoro or chloro, for example in the 4-position. In one embodiment R3a and R3b are in the 3,4 position respectively. In one embodiment R4 is H.
In one embodiment R5 is H.
In one embodiment p is 2. In one embodiment q is 2.
In one embodiment there is a provided a pharmaceutically acceptable acid addition salt of a compound of formula (I). The pharmaceutically acceptable acid addition salts as mentioned hereinabove are meant to comprise the therapeutically active non-toxic acid addition salt forms that the compounds of formula (I) are able to form. These pharmaceutically acceptable acid addition salts can conveniently be obtained by treating the base form with such appropriate acid. Appropriate acids comprise, for example, inorganic acids such as hydrohalic acids, e.g. hydrochloric or hydrobromic acid, sulfuric, nitric, phosphoric and the like acids; or organic acids such as, for example, acetic, propanoic, hydroxyacetic, lactic, pyruvic, oxalic (i.e. ethanedioic), malonic, succinic (i.e. butanedioic acid), maleic, fumaric, malic, tartaric, citric, methanesulfonic, ethanesulfonic, benzenesulfonic, p-toluenesulfonic, cyclamic, salicylic, p-aminosalicylic, pamoic and the like acids.
Examples of salts of compound (I) include all pharmaceutically acceptable salts, such as, without limitation, acid addition salts of mineral acids such as HCI and HBr salts and addition salts of organic acids such as a methansulfonic acid salt. The disclosure also extends to solvates of the compounds herein. Examples of solvates include hydrates. The compounds of the disclosure include those where the atom specified is a naturally occurring or non-naturally occurring isotope. In one embodiment the isotope is a stable isotope. Thus the compounds of the disclosure include, for example those containing one or more deuterium atoms in place of hydrogen atoms and the like.
The compounds described herein may include one or more chiral centres, and the disclosure extends to include racemates, both enantiomers (for example each substantially free of the other enantiomer) and all stereoisomers resulting therefrom. In one embodiment one enantiomeric form is present in a purified form that is substantially free of the corresponding entaniomeric form.
The disclosure also extends to all polymorphic forms of the compounds herein defined.
In one aspect a compound of formula (I) is:
2-((4-Amino-3-(3-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-5-ethynylquinazolin-4(3/-/)-one;
2-((4-Amino-3-(3-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-5-(3-(2-(2-methoxyethoxy)ethoxy)prop-1 -yn-1 -yl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(3-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-5-(6-morpholino-6-oxohex-1 -yn-1 -yl)quinazolin-4(3/-/)-one; 6-(2-((4-Amino-3-(4-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)hex-5-ynoic acid;
2- ((4-Amino-3-(4-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-5-(6-morpholino-6-oxohex-1 -yn-1 -yl)quinazolin-4(3/-/)-one;
3- ((2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-5-(3-(2-(2- hydroxyethoxy)ethoxy)prop-1 -yn-1 -yl)-4-oxoquinazolin-3(4/-/)-yl)methyl)benzonitrile;
2-((4-amino-3-(3-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chlorobenzyl) -5-(3-(2-morpholinoethoxy)prop-1 -ynyl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-5-ethynylquinazolin-4(3/-/)-one; 2-((4-Amino-3-(3-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(3-chloro benzyl) -5-ethynylquinazolin-4(3/-/)-one; 2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin- -yl)methyl i-3-(3-chloro benzyl) -5-ethynylquinazolin-4(3/-/)-one;
2-((4-Amino-3-(3-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin- -yl)methyl i-5-ethynyl-3-(2- fluorobenzyl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin- -yl)methyl i-5-ethynyl-3-(2- fluorobenzyl)quinazolin-4(3/-/)-one; 2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin- -yl)methyl i-5-ethynyl-3-(3- methoxybenzyl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(3-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin- -yl)methyl i-5-ethynyl-3-(3- methoxybenzyl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin- -yl)methyl i-5-ethynyl-3-(3- (trifluoromethyl)benzyl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(3-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin- -yl)methyl i-5-ethynyl-3-(3- (trifluoromethyl)benzyl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin- -yl)methyl i-3-(4-chloro benzyl) -5-ethynyl quinazolin-4(3/-/)-one.; 2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin- -yl)methyl i-5-ethynyl-3-(4- (methylsulfonyl)benzyl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin- -yl)methyl i-5-ethynyl-3-(4- (methylsulfonyl)benzyl)quinazolin-4(3/-/)-one;
2- ((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-(i]pyrimidin-1 -yl)methyl i-5-ethynyl-3-(4- (trifluoromethyl)benzyl)quinazolin-4(3/-/)-one;
3- ((2-((4-Amino-3-(4-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin-1 -yl)me hyl)-5-ethynyl-4- oxoquinazolin-3(4H)-yl)methyl)benzonitrile;
2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-(i]pyrimidin-1 -yl)methyl i-5-ethynyl-3-(3- (methylsulfonyl)benzyl)quinazolin-4(3/-/)-one; 3-((2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)me hyl)-5-ethynyl-4- oxoquinazolin-3(4/-/)-yl)methyl)benzonitrile; 2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(4-ch benzyl) -5-ethynylquinazolin-4(3/-/)-one;
2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(4-chloro benzyl) -5-(3-methoxyprop-1 -ynyl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-(^pyrimidin-1 -yl)methyl)-3-(3-methoxy benzyl)-5-(3-methoxyprop-1 -ynyl)quinazolin-4(3/-/)-one; 2-((4-Amino-3-(4-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl) -5-(3-methoxyprop-1 -ynyl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-5-ethynyl-3-(4- (trifluoromethyl)benzyl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl) -5-(3-(2-methoxyethoxy)prop-1 -ynyl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-5-ethynyl-3-((5- methylisoxazol-3-yl)methyl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-5-ethynyl-3-((5- methylisoxazol-3-yl)methyl)quinazolin-4(3/-/)-one; 2-((4-Amino-3-(4-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(3-chloro-2- fluorobenzyl)-5-ethynylquinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2,6-difluoro benzyl)-5-ethynylquinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(4-chloro-2- fluorobenzyl)-5-ethynylquinazolin-4(3/-/)-one;
2-((4-Amino-3-(3-fluoro-4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2- chlorobenzyl)-5-ethynylquinazolin-4(3/-/)-one;
2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-(^pyrimidin-1 -yl)methyl)-5-(3-methoxy prop-1 -ynyl)-3-(3-(trifluoromethyl)benzyl)quinazolin-4(3/-/)-one; 2-((4-Amino-3-(4-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-5-ethynyl-3-(4- fluorobenzyl) quinazolin-4(3/-/)-one; 2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-ch benzyl)-5-(3-cyclopentylprop-1 -ynyl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-(^pyrimidin-1 -yl)methyl)-5-(3-(benzyloxy) prop-1 -ynyl)-3-(2-chlorobenzyl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl) -5-(5-hydroxypent-1 -ynyl)quinazolin-4(3/-/)-one; 2-((4-Amino-3-(4-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-5-ethynyl-3-(2- fluoro-5-methoxybenzyl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-^
benzyl)-5-ethynylquinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-(^pyrimidin-1 -yl)methyl)-3-benzyl-5- ethynylquinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-5-ethynyl-3 trifluoromethylbenzyl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-5-ethynyl-3 methoxybenzyl)quinazolin-4(3/-/)-one; 4-((2-((4-Amino-3-(4-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-5-ethynyl-4- oxoquinazolin-3(4/-/)-yl)methyl)benzonitrile;
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-5-ethynyl-3 fluoro-4-methoxybenzyl)quinazolin-4(3/-/)-one;
1 -(3-(2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c ]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)prop-2-ynyl)urea;
2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-(^pyrimidin-1 -yl)methyl)-3-(2-fluoroben -5-(3-(2-(2-methoxyethoxy)ethoxy)prop-1 -ynyl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-fluoro-3-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2- chlorobenzyl)-5-ethynylquinazolin-4(3/-/)-one; 2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-5-(3-phenoxyprop-1 -ynyl)quinazolin-4(3/-/)-one; 2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-fl -5-(6-morpholino-6-oxohex-1 -yn-1 -yl)quinazolin-4(3/-/)-one;
6-(2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)-/V-(2-methoxyethyl)hex-5-ynamide;
2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-5-(7-morpholino-7-oxohept-1 -yn-1 -yl)quinazolin-4(3/-/)-one; 2-((4-Amino-3-(3-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-5-(5-morpholino-5-oxopent-1 -yn-1 -yl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-d7pyi"iniidin-1 -yl)methyl)-3-((5-methyl pyrazin-2-yl)methyl)-5-(6-morpholino-6-oxohex-1 -yn-1 -yl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-5-(6-oxo-6-(piperidin-1 -yl)hex-1 -yn-1 -yl)quinazolin-4(3/-/)-one;
6- (2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)-/V,/V-diethylhex-5-ynamide;
7- (2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)hept-6-ynoic acid; 2-Acetamido-N-(3-(2-((4-amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl) methyl)-3-(2-chlorobenzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)prop-2-yn-1 -yl)acetamide;
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-(^pyrimidin-1 -yl)methyl)-3-(3-meth
(trifluoromethyl)benzyl)-5-(6-morpholino-6-oxohex-1 -yn-1 -yl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-(^pyrimidin-1 -yl)methyl)-3-(2-meth phenethyl)-5-(6-morpholino-6-oxohex-1 -yn-1 -yl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-(^pyrimidin-1 -yl)methyl)-3-(benzo[b]
2-ylmethyl)-5-(6-morpholino-6-oxohex-1 -yn-1 -yl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-fluoro-3- methoxybenzyl)-5-(6-morpholino-6-oxohex-1 -yn-1-yl)quinazolin-4(3/-/)-one; Methyl 3-((2-((4-amino-3-(3-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-5-(6- morpholino-6-oxohex-1 -yn-1 -yl)-4-oxoquinazolin-3(4/-/)-yl)methyl)benzoate; 2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-((1 -methyl-1 H- pyrazol-4-yl)methyl)-5-(6-morpholino-6-oxohex-1 -yn-1 -yl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-<^
ylmethyl)-5-(6-morpholino-6-oxohex-1 -yn-1 -yl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-((2-methyl thiazol-4-yl)methyl)-5-(6-morpholino-6-oxohex-1 -yn-1 -yl)quinazolin-4(3/-/)-one; 2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-5-(6-(4-methylpiperazin-1 -yl)-6-oxohex-1 -yn-1 -yl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-5-(6-(4-morpholinopiperidin-1 -yl)-6-oxohex-1 -yn-1 -yl)quinazolin-4(3/-/)-one;
5-(6-(4-Acetylpiperazin-1 -yl)-6-oxohex-1 -yn-1 -yl)-2-((4-amino-3-(4-hydroxyphenyl)-1 H- pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chlorobenzyl)quinazolin-4(3/-/)-one;
/V-(4-(2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-^
benzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)but-3-yn-1 -yl)morpholine-4-carboxamide;
5- (6-(4-Acetylpiperazin-1 -yl)-6-oxohex-1 -yn-1 -yl)-2-((4-amino-3-(4-hydroxyphenyl)-1 H- pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chlorobenzyl)quinazolin-4(3/-/)-one; /V-(4-(2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-c ]pynmidin-1 -yl)methyl)-3-(2- chlorobenzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)but-3-yn-1 -yl)morpholine-4-carboxamide;
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-5-(5-(bis(2- methoxyethyl)amino)pent-1 -ynyl)-3-(2-chlorobenzyl)quinazolin-4(3/-/)-one;
6- (2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)-/V-cyclopentylhex-5-ynamide;
6-(2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)-/V-(tetrahydro-2/-/-pyran-4-yl)hex-5-ynamide;
6-(2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-d7pynniidin-1 -yl)methyl)-3-(2-chloro benzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)-/V-(2-morpholinoethyl)hex-5-ynamide; 2-((4-Amino-3-(4-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-5-(6-(4-(2-methoxyethyl)piperazin-1 -yl)-6-oxohex-1 -ynyl)quinazolin-4(3/-/)-one; 6-(2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2 benzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)-/V-(2-(dimethylamino)ethyl)hex-5-ynamide;
6-(2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)-/V-(pyridin-4-yl)hex-5-ynamide;
6-(2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)-/V-(pyridin-4-yl)hex-5-ynamide; 2-((4-Amino-3-(4-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-5-(6-(4-(dimethylamino)piperidin-1 -yl)-6-oxohex-1 -ynyl)quinazolin-4(3/-/)-one;
6-(2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)-/V,/V-bis(2-methoxyethyl)hex-5-ynamide;
6-(2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)-/V,/V-bis(2-methoxyethyl)hex-5-ynamide;
6-(2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)-/V-(2-(4-methylpiperazin-1 -yl)ethyl)hex-5-ynami
6-(2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)-/V-methyl-/V-(2-(4-methylpiperazin-1 -yl)ethyl)h ynamide;
6-(2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)-/V-isopropylhex-5-ynamide;
6-(2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)-/V-isopropylhex-5-ynamide;
6-(2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)-/V,/V-dimethylhex-5-ynamide; 2-((4-Amino-3-(4-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-5-(6-oxo-6-(pyrrolidin-1 -yl)hex-1 -yn-1 -yl)quinazolin-4(3/-/)-one;
6-(2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)-/V-(pyrrolidin-3-yl)hex-5-ynamide;
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-5-(6-(3-(dimethylamino)pyrrolidin-1 -yl)-6-oxohex-1 -ynyl)quinazolin-4(3/-/)-one; 2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)meth
benzyl)-5-(6-(3-(dimethylamino)pyrrolidm^
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-ch
benzyl)-5-(6-(4-methyl-1 ,4-diazepan-1 -yl)-6-oxohex-1 -ynyl)quinazolin-4(3/-/)-one,
2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-ch
benzyl)-5-(6-(4-methyl-1 ,4-diazepan-1 -yl)-6-oxohex-1 -ynyl)quinazolin-4(3/-/)-one, 2-((4-Amino-3-(4-hydroxy-3-methoxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2- chlorobenzyl)-5-(6-morpholino-6-oxohex-1 -ynyl)quinazolin-4(3/-/)-one
or a pharmaceutically acceptable salt thereof, including all stereoisomers, tautomers and isotopic derivatives thereof. Compounds of formula (I) can be prepared by a process comprising reacting compounds of formula (II):
Figure imgf000030_0001
or a protected derivate thereof wherein X, R3a, R3b,R4 and R5 are as defined above for compounds of formula (I) and LG-i represents a leaving group such as halo, in particular bromo, with a compound of formula (III):
(Ill) or a protected derivative thereof, wherein R1 is as defined above for compounds of formula (I), in the presence of a suitable catalyst, an organic base and a polar aprotic solvent, under an inert atmosphere; and optionally deprotecting to reveal a compound of formula (I); and optionally converting one compound of formula (I) into another compound of formula (I) by standard functional group transformations.
Suitable catalysts include palladium catalysts such as t»/s(triphenylphosphine)palladium (II) dichloride, in the presence of copper iodide. A suitable polar aprotic solvent is DMF.
A suitable inert atmosphere is nitrogen. The reaction may be performed employing irradiation, for example using microwave irradiation at 120°C and a power setting of 200W.
The deprotection, to reveal a compound of formula (I) where, for example R1 is H, and the protective group is a silyl group can be effected by treatment with a reagent such as tetrabutylammonium chloride in the presence of a polar aprotic solvent such as DMF. The reaction may be performed at a reduced temperature, such as about 0°C.
Compounds of formula (I) wherein R1 comprises a carboxylic acid moiety can be converted to other compounds of formula (I) by routine techniques, for example amide couplings.
Further examples of converting a compound of formula (I) into other compounds of formula (I) are provided by those compounds of formula (I) wherein R1 comprises a primary alcohol. Such compounds may be transformed into compounds of formula (I) wherein R1 comprises a primary alkyl halide, and then, by subsequent reaction with a primary or secondary amine, into a compound of formula (I) wherein R1 comprises an alkyl amine.
Compounds of formula (II) can be prepared by reacting a compound of formula (IV):
Figure imgf000031_0001
or a protected derivative thereof, wherein LGi and X are as defined above for compounds of formula (II) and LG2 is a leaving group, for example halo, such as chloro, with a compound of formula (V):
Figure imgf000031_0002
or a protected derivative thereof, wherein R3a, R3b, R4 and R5 are as defined above for compounds of formula (I), in the presence of a base, in a polar aprotic solvent. Suitable protecting groups for compounds of formula (V) in which R is hydroxyl include a ie fbutyldimethylsilylether.
Suitable bases include potassium carbonate.
A suitable polar aprotic solvent is DMF.
Alternatively compounds of formula (II) can be prepared by reacting a compound of formula
(VI):
Figure imgf000032_0001
or a protected derivative thereof, wherein LG-i , X, R4 and R5 are as defined above for compounds of formula (II) and LG3 represents a leaving group such as halo, in particular iodo, with a compound of formula (VII):
Figure imgf000032_0002
(VII) or a protected derivate thereof, wherein R and R are as defined above for compounds of formula (I), in the presence of a suitable noble metal catalyst, an inorganic base and a polar protic solvent, under an inert atmosphere; followed, where appropriate, by deprotection of the groups R3a and/or R3b, and /or X.
A suitable catalyst is tetrakis(triphenylphosphine)palladium(0).
A suitable inorganic base is sodium carbonate.
A suitable polar protic solvent is ethanol.
The reaction may be performed at an elevated temperature, for example at 85°C for several days for example 3 days before cooling to RT. Compounds of formula (VI) can be prepared by reacting a compound of formula (IV) or a protected derivative thereof, wherein LGi LG2 and X are as defined above with a compound of formula (VIII):
Figure imgf000033_0001
(VIII) or a protected derivative thereof, wherein R4 and R5 are as defined above for compounds of formula (I) and LG3 is a leaving group, for example a halogen atom, in particular an iodo substituent. The reaction is conveniently conducted in a polar aprotic solvent and in the presence of an inorganic base at ambient temperature, such as RT and in the dark.
A suitable inorganic base is sodium carbonate.
A suitable polar aprotic solvent is DMF.
Compounds of formula (V) wherein R3a, R3b, R4 and R5 are as defined above, may be prepared by reacting a compound of formula (VIII) wherein LG3, R4 and R5 are as defined above with a compound of formula (VII) or a protected derivate thereof, wherein R3a and R3b are as defined above for compounds of formula (I):
Compounds of formula (IV) may be prepared by reacting a compound of formula (IX):
Figure imgf000033_0002
or a protected derivative thereof wherein LGi is as defined above for compounds of formula (II) and LG4 is a leaving group, for example halo, such as chloro, or hydroxy with a compound of formula (X):
Figure imgf000033_0003
or a protected derivative thereof, wherein X, R and R are as defined above for compounds of formula (II), in the presence of a suitable reagent, such as a phosphorus trihalide, an organic base and a non-polar solvent. A suitable phosphorus trihalide is phosphorus trichloride.
A suitable non-polar solvent is toluene.
A suitable organic base is triethylamine.
Protection of the hydroxyl may, for example, be effected using TBDMSCI in a suitable solvent, for example DMF in the presence of a suitable base such as imidazole.
Compounds of formula (IX) may be prepared by reacting a compound of formula (XI):
Figure imgf000034_0001
or a protected derivative thereof, wherein LG-i is as defined above for compounds of formula (II) with a compound of formula (XII):
Figure imgf000034_0002
wherein LG5 and LG6 are leaving groups, for example halo, such as chloro in presence of a suitable solvent and a suitable base .
A suitable solvent is toluene and a suitable base is pyridine.
Protecting groups may be required to protect chemically sensitive groups during one or more of the reactions described above, to ensure that the process is efficient. Thus if desired or necessary, intermediate compounds may be protected by the use of conventional protecting groups. Protecting groups and means for their removal are described in "Protective Groups in Organic Synthesis", by Theodora W. Greene and Peter G.M. Wuts, published by John Wiley & Sons Inc; 4th Rev Ed., 2006, ISBN-10: 0471697540.
Novel intermediates are claimed as an aspect of the invention. The compounds of formulae (III), (VII), (VIII), (X) and (XI) and (XII) as defined above, are either commercially available or can be readily prepared from commericially available starting materials, using routine published techniques (see for example Table 1 ). A selection of the compounds of formula (I), disclosed herein, have been screened by chiral stationary phase HPLC for the presence of non interconverting rotational isomers, otherwise known as atropisomers. Atropisomerism results from hindered rotation about a single bond where the torsional energy barrier to free rotation is sufficiently high to significantly slow the interconversion of non-superimposable conformers. The analytical and preparative methods disclosed below have been used to resolve the discrete atropisomers of a number of published PI3K inhibitors, revealing that they ordinarily occur as mixtures of stereoisomers possessing very long half-lives (months or years) under physiological conditions. (See experimental section for details). The occurence of atropisomerism represents an added burden of complexity to the development of such compounds as therapeutic agents, since discrete stereoisomers are usually highly preferred over mixtures, such as racemates. Analysis of selected examples using the chromatographic methods disclosed herein have revealed no evidence that the compounds of formula (I) exibit atropisomerism. In the absence of additional features that are well known to give rise to stereoisomers, for example a stereogenic centre, the current disclosure provides for compounds that advantageously exist as single discrete molecular entities. Thus the compounds of the present disclosure are particularly useful as therapeutically active agents in pharmaceutical compositions and in treatment.
In one aspect the compounds are useful in treatment, for example COPD and/or asthma. The PI3K compounds developed to date have typically been intended for oral administration. Typically this strategy involves the optimisation of a compound's pharmacokinetic profile in order to achieve an adequate duration of action. In this way a sufficiently high drug concentration is established and maintained between doses to provide continuous clinical benefit. An inevitable and frequently undesired consequence of this approach is that non- targeted body tissues, especially the liver and the gut, are likely to be exposed to pharmacologically active concentrations of the drug.
An alternative strategy is to design treatment regimens in which the drug is dosed directly to the inflamed organ (for example topical therapy). Although this approach is not suitable for treating all chronic inflammatory conditions, it has been extensively exploited in treating lung diseases (asthma, COPD), skin lesions (atopic dermatitis and psoriasis), nasal diseases (allergic rhinitis) and gastrointestinal disorders (ulcerative colitis).
In topical therapy, the desired efficacy can sometimes be achieved by ensuring that the drug has a sustained duration of action and is retained predominantly in the target organ, thereby minimising the risks of systemic toxicity. Alternatively an appropriate formulation can be used which generates a "reservoir" of the active drug which is then available to sustain the desired effects. The first approach is exemplified in the use of the anticholinergic drug tiotropium bromide (Spiriva HandiHaler®), which is administered topically to the lung as a treatment for COPD.This compound has an exceptionally high affinity for its target receptor resulting in a very slow off rate (dissociation rate) and a consequent sustained duration of action. There is provided according to one aspect of the present disclosure use of a compound of formulation as a PI3 kinase inhibitor, for example administered topically to the lung.
In one aspect of the disclosure the compounds herein are particularly suitable for topical delivery, such as topical delivery to the lungs, in particular for the treatment of COPD.
Thus is one aspect there is provided use of compounds of formula (I) for the treatment of COPD and/or asthma, in particular COPD or severe asthma, by inhalation i.e. topical administration to the lung. Advantageously, administration to the lung allows the beneficial effects of the compounds to be realised whilst minimising the side-effects, for patients.
In one embodiment the compounds are suitable for sensitizing patients to treatment with a corticosteroid.
The compounds herein may also be useful for the treatment of rheumatoid arthritis.
Further, the present invention provides a pharmaceutical composition comprising a compound according to the disclosure optionally in combination with one or more pharmaceutically acceptable diluents or carriers. Diluents and carriers may include those suitable for parenteral, oral, topical, mucosal and rectal administration, and may be different depending on the route of administration.
In one embodiment compositions may be prepared e.g. for parenteral, subcutaneous, intramuscular, intravenous, intra-articular or peri-articular administration, particularly in the form of liquid solutions or suspensions; for oral administration, particularly in the form of tablets or capsules; for topical e.g. pulmonary or intranasal administration, particularly in the form of powders, nasal drops or aerosols and transdermal administration; for mucosal administration e.g. to buccal, sublingual or vaginal mucosa, and for rectal administration e.g. in the form of a suppository.
The compositions may conveniently be administered in unit dosage form and may be prepared by any of the methods well-known in the pharmaceutical art, for example as described in Remington's Pharmaceutical Sciences, 17th ed., Mack Publishing Company, Easton, PA., (1985).
Formulations for parenteral administration may contain as excipients sterile water or saline, alkylene glycols such as propylene glycol, polyalkylene glycols such as polyethylene glycol, oils of vegetable origin, hydrogenated naphthalenes and the like. Formulations for nasal administration may be solid and may contain excipients, for example, lactose or dextran, or may be aqueous or oily solutions for use in the form of nasal drops or metered spray. For buccal administration typical excipients include sugars, calcium stearate, magnesium stearate, pregelatinated starch, and the like.
Compositions suitable for oral administration may comprise one or more physiologically compatible carriers and/or excipients and may be in solid or liquid form. Tablets and capsules may be prepared with binding agents, for example, syrup, acacia, gelatin, sorbitol, tragacanth, or poly-vinylpyrollidone; fillers, such as lactose, sucrose, corn starch, calcium phosphate, sorbitol, or glycine; lubricants, such as magnesium stearate, talc, polyethylene glycol, or silica; and surfactants, such as sodium lauryl sulfate. Liquid compositions may contain conventional additives such as suspending agents, for example sorbitol syrup, methyl cellulose, sugar syrup, gelatin, carboxymethyl-cellulose, or edible fats; emulsifying agents such as lecithin, or acacia; vegetable oils such as almond oil, coconut oil, cod liver oil, or peanut oil; preservatives such as butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT). Liquid compositions may be encapsulated in, for example, gelatin to provide a unit dosage form.
Solid oral dosage forms include tablets, two-piece hard shell capsules and soft elastic gelatin (SEG) capsules.
A dry shell formulation typically comprises of about 40% to 60% concentration of gelatin, about a 20% to 30% concentration of plasticizer (such as glycerin, sorbitol or propylene glycol) and about a 30% to 40% concentration of water. Other materials such as preservatives, dyes, opacifiers and flavours also may be present. The liquid fill material comprises a solid drug that has been dissolved, solubilized or dispersed (with suspending agents such as beeswax, hydrogenated castor oil or polyethylene glycol 4000) or a liquid drug in vehicles or combinations of vehicles such as mineral oil, vegetable oils, triglycerides, glycols, polyols and surface-active agents.
Suitably the compound of formula (I) is administered topically to the lung. Hence in one embodiment there is provided a pharmaceutical composition comprising a compound of the disclosure optionally in combination with one or more topically acceptable diluents or carriers. Topical administration to the lung may be achieved by use of an aerosol formulation. Aerosol formulations typically comprise the active ingredient suspended or dissolved in a suitable aerosol propellant, such as a chlorofluorocarbon (CFC) or a hydrofluorocarbon (HFC). Suitable CFC propellants include trichloromonofluoromethane (propellant 1 1 ), dichlorotetrafluoromethane (propellant 1 14), and dichlorodifluoromethane (propellant 12). Suitable HFC propellants include tetrafluoroethane (HFC-134a) and heptafluoropropane (HFC- 227). The propellant typically comprises 40% to 99.5% e.g. 40% to 90% by weight of the total inhalation composition. The formulation may comprise excipients including co-solvents (e.g. ethanol) and surfactants (e.g. lecithin, sorbitan trioleate and the like). Aerosol formulations are packaged in canisters and a suitable dose is delivered by means of a metering valve (e.g. as supplied by Bespak, Valois or 3M).
Topical administration to the lung may also be achieved by use of a non-pressurised formulation such as an aqueous solution or suspension. This may be administered by means of a nebuliser. Topical administration to the lung may also be achieved by use of a dry-powder formulation. A dry powder formulation will contain the compound of the disclosure in finely divided form, typically with a mass mean diameter (MMAD) of 1 -10 μηη. The formulation will typically contain a topically acceptable diluent such as lactose, usually of large particle size e.g. a mass mean diameter (MMAD) of 100 μηη or more. Example dry powder delivery systems include SPINHALER, DISKHALER, TURBOHALER, DISKUS, SKYEHALER, ACCUHALER and CLICKHALER.
In one embodiment a compound of the present is provided as a micronized dry powder formulation, for example comprising lactose of a suitable grade, filled into a device such as DISKUS.
Compounds according to the disclosure are intended to have therapeutic activity. In a further aspect, the present invention provides a compound of the disclosure for use as a medicament.
Compounds according to the disclosure may also be useful in the treatment of respiratory disorders including COPD (including chronic bronchitis and emphysema), asthma, paediatric asthma, cystic fibrosis, sarcoidosis, idiopathic pulmonary fibrosis, allergic rhinitis, rhinitis, sinusitis, especially asthma, chronic bronchitis and COPD.
Compounds of the disclosure may also re-sensitise the patient's condition to treatment with a corticosteroid, when the patient's condition has become refractory to the same.
One or more compounds of the disclosure may exhibit anti-viral activity and prove useful in the treatment of viral exacerbations of asthma and/or COPD.
The compounds of the present disclosure may also be useful in the prophylaxis, treatment or amelioration of flu virus, rhinovirus and/or respiratory syncytical virus. Compounds according to the disclosure are also expected to be useful in the treatment of certain conditions which may be treated by topical or local therapy including allergic conjunctivitis, conjunctivitis, allergic dermatitis, contact dermatitis, psoriasis, ulcerative colitis, inflamed joints secondary to rheumatoid arthritis or osteoarthritis. In one embodiment the compounds of formula (I) are thought to be useful in the, treatment of Hepatitis C and/or HIV, when administered by an appropriate route. Appropriate routes of administration may include oral, intravenous injection or infusion. In one embodiment a compound of formula (I) for the treatment of Hepatitis C is delivered to the blood pre-entry to the liver.
Compounds of the disclosure are also expected to be useful in the treatment of certain other conditions including rheumatoid arthritis, pancreatitis, cachexia, inhibition of the growth and metastasis of tumours including non-small cell lung carcinoma, breast carcinoma, gastric carcinoma, colorectal carcinomas and malignant melanoma.
Thus, in a further aspect, the present invention provides a compound as described herein for use in the treatment of one or more of the above mentioned conditions.
In a further aspect, the present invention provides use of a compound as described herein for the manufacture of a medicament for the treatment of one or more of the above mentioned conditions.
In a further aspect, the present invention provides a method of treatment of the above mentioned conditions which comprises administering to a subject an effective amount of a compound of the disclosure or a pharmaceutical composition thereof. Compounds described herein may also be used in the manufacture of a medicament for the treatment of one or more of the above-identified diseases.
The word "treatment" is intended to embrace prophylaxis as well as therapeutic treatment. A compound of the disclosure may also be administered in combination with one or more other active ingredients e.g. active ingredients suitable for treating the above mentioned conditions. For example possible combinations for treatment of respiratory disorders include combinations with steroids (e.g. budesonide, beclomethasone dipropionate, fluticasone propionate, mometasone furoate, fluticasone furoate), beta agonists (e.g. terbutaline, salbutamol, salmeterol, formoterol, indacaterol) and/or xanthines (e.g. theophylline), musacarinic antagonists, (e.g. ipratropium) and/or a p38 MAP kinase inhibitor.
In one embodiment a compound of the disclosure is administered in combination with an antiviral agent, for example acyclovir, tamiflu, relenza or interferon.
In one embodiment the combination of active ingredients are co-formulated.
In one embodiment the combination of active ingredients is simply co-administered.
EXPERIMENTAL SECTION
Abbreviations Abbreviations used herein have the meanings defined in the table below. Any abbreviations appearing in the text which have not been defined are intended to covey their generally accepted meaning.
AcOH glacial acetic acid
Aq aqueous
Ac Acetyl
ATP adenosine-5'-triphosphate
BALF bronchoalveolae lavage fluid
Boc ie f-butoxycarbonyl
Br Broad
BSA bovine serum albumin
CDI 1 ,1 -carbonyl-diimidazole
COPD chronic obstructive pulmonary disease
D Doublet
DCM dichloromethane
DIAD diisopropylazadicarboxylate
DIBAL-H diisobutylaluminium hydride
DIPEA Λ/,/V-diisopropylethylamine
DMF Λ/,/V-dimethylformamide
DMSO dimethyl sulfoxide
dppf 1 ,1 '-b/'s(diphenylphosphino)ferrocene
EDC 1 -ethyl-3-(3-dimethylaminopropyl)carbodiimide
(ES+) electrospray ionization, positive mode
Et Ethyl
Et20 diethyl ether
Et3N triethylamine
EtOH Ethanol
EtOAc ethyl acetate
FACS fluorescence-activated cell sorting
FCS foetal calf serum
G gram(s)
HOBt 1 -hydroxybenzotriazole
HPLC high performance liquid chromatography
HPLC-MS high performance liquid chromatography mass spectrometry
Hr hour(s)
HRP horseradish peroxidase
i-n intra-nasal
i-t intra-tracheal
KHMDS potassium hexamethyldisilazane
μΙ_ microliter(s)
LPS lipopolysaccharide
μΜ micromolar Mm micro metre
M Molar
(M+H)+ protonated molecular ion
Me Methyl
MeOH methanol
MeOD deuterated methanol
Mg milligram(s)
MHz megahertz
Min minute(s)
mL millilitre(s)
mM millimolar
Mm millimetre
mmol millimole(s)
MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide m/z mass-to-charge ratio
Ng nanogram
Nm nanomolar
Nm nanometre
NMP 1 -methylpyrrolidin-2-one (/V-methyl-2-pyrrolidone)
NMR nuclear magnetic resonance (spectroscopy)
PBS phosphate buffered saline
Pd2(dba)3 ir/'s(dibenzylideneacetone)dipalladium(0)
Ph Phenyl
PIP2 phosphatidylinositol 4,5-biphosphate
PIP3 phosphatidylinositol 3,4,5-triphosphate
PMA phorbol myristate acetate
Po by oral administration
PPh3 triphenylphosphine
PyBOP® (benzotriazol-1 -yloxy)tripyrrolidinophosphonium hexafluorophosphate
Q Quartet
Quin Quintet
R' retention time
RT room temperature
RP HPLC reverse phase high performance liquid chromatography
S Singlet
SCX solid supported cation exchange (resin)
SDS sodium dodecyl sulfate
T Triplet
TBAF tetrabutylammonium fluoride
TBDMSCI ie/f-butyldimethylsilyl chloride
TFA trifluoroacetic acid
THF tetrahydrofuran
TLC thin-layer chromatography TMB 3,3',5,5'-tetramethylbenzidine
TNFa tumour necrosis factor alpha
TR-FRET time-resolved fluorescence resonance energy transfer
Vol Volume
VT Variable temperature
W Watts
General Procedures
Those intermediates and bespoke chemical building blocks not available from commercial sources were prepared according to the procedures disclosed herein or using the cited literature methods. All other starting materials and solvents (HPLC grade) were obtained from commercial sources and were used without further purification Where indicated reactions were degassed by passing a stream of nitrogen through them for at least 10 min.. Microwave reactions were carried out using a CEM Discover or Explorer focussed microwaves apparatus. Organic solutions were dried using magnesium sulphate or sodium sulphate. Flash column chromatography was performed using a minimum of 20 mass equivalents of silica with SiliCycle SiliaFlash® P60 (230-400 mesh) or a Biotage Isolute SPE Column prepacked with Flash Si II.
Analytical Methods
Thin-layer chromatography (TLC) analysis was performed with Kieselgel 60 with fluorescence indicator UV254 plates and visualized using UV light or stained with standard TLC dips or iodine.
Reverse Phase Analytical High Performance Liquid Chromatography-Mass Spectrometry:
All samples were routinely analysed by HPLC-MS using the instrumentation and conditions described below. Unless stated otherwise final compound were subject to analysis using Method B and intermediates were analysed utilising Method A. Method A was performed on Agilent HP1 100 and Shimadzu 2010, systems using reverse phase Atlantis dC18 columns (5 μ m, 2.1 x 50 mm), gradient 5-100% B (A = water/ 0.1 % formic acid, B = acetonitrile/ 0.1 % formic acid) over 3 min, injection volume 3 L, flow = 1 .0 mL/min. UV spectra were recorded at 215 nm using a Waters 2487 dual wavelength UV detector or the Shimadzu 2010 system. Mass spectra were obtained over the range m/z 150 to 850 at a sampling rate of 2 scans per second using Waters ZMD or over m/z 100 to 1000 at a sampling rate of 2 Hz using electrospray ionisation, by a Shimadzu 2010 LC-MS system.
Method B was performed on Agilent HP1 100 and Shimadzu 2010, systems using reverse phase Water Atlantis dC18 columns (3 μ m, 2.1 x 100 mm), gradient 5-100% B (A = water/ 0.1 % formic acid, B = acetonitrile/ 0.1 % formic acid) over 7 min, injection volume 3 μ L, flow = 0.6 mL/min. UV spectra were recorded at 215 nm using a Waters 2996 photo diode array or on the Shimadzu 2010 system. Mass spectra were obtained over the range m/z 150 to 850 at a sampling rate of 2 scans per second using Waters ZQ or over m/z 100 to 1000 at a sampling rate of 2 Hz using electrospray ionisation, by a Shimadzu 2010 LC-MS system.
Method C was performed on Agilent HP1 100, systems using reverse phase Phenomenex Gemini dC18 columns (3 μ m, 2.0 x 50 mm), gradient 1 -100% B (A = 2 mM ammonium bicarbonate pH10, B = acetonitrile) over 3.5 min, injection volume 3 μ L, flow = 1 .0 mL/min. UV spectra were recorded at 215 nm using a Waters 2487 dual wavelength UV detector or a Waters 2996 photo diode array. Mass spectra were obtained over the range m/z 150 to 850 at a sampling rate of 2 scans per second using Waters ZMD or a Waters ZQ.
Method D: was performed on Agilent HP1200, systems using Agilent Extend C18 columns, (1 .8 μηη, 4.6 x 30 mm) at 40°C and a flow rate of 2.5-4.5 mL min"1, eluting with a H20-MeCN gradient containing 0.1 % v/v formic acid over 4 min. Gradient information: 0-3.00 min, ramped from 95% H20-5% MeCN to 5% H20-95% MeCN; 3.00-3.01 min, held at 5% H20-95% MeCN, flow rate increased to 4.5 mL.min"1; 3.01-3.50 min, held at 5% H20-95% MeCN; 3.50-3.60 min, returned to 95% H20-5% MeCN; flow rate reduced to 3.50 mL min"1; 3.60-3.90 min, held at 95% H20-5% MeCN; 3.90-4.00 min, held at 95% H20-5% MeCN, flow rate reduced to 2.5 mL.min"1. UV detection was performed at 254 nm using an Agilent G1314B variable wavelength detector. Mass spectra were obtained over the range m/z 60 to 2000 at a sampling rate of 1 .6 sec/cycle using an Agilent G1956B.
Chiral Stationary Phase Analytical HPLC was performed using Daicel columns (5 pm x 5 cm x 4.6 mm) containing one of the following stationary phases: Chiralpak AD-H, Chiralcel OD-H, Chiralcel OJ-H, and Chiralpak AS-H. A flow rate of 1 mL/min, was used eluting under isocratic conditons over 6 to 20 min, with one of the following mobile phases: 70:30 heptane:IPA (v/v), 85:15 heptane:EtOH (v/v), 50:50 MeOH:EtOH (v/v), and acetonitrile; employing UV detection at 254 nm. Each of these mobile phase are compatible with the column packings referred to above, providing sixteen different solvent and stationary phase permutations. Modifers such as triethylamine (0.1 %), formic acid (0.1 %) may be added to improve the resolution
Semi-preparative Chiral Stationary Phase LC-UV was performed on a Gilson preparative modular system using a Daicel Chiralpak AD semi-prep column (10 mm, 20 x 250 mm) and a flow rate of 18 mL/min eluting with an isocratic method over 20 min using 70:20 heptane:IPA and 0.1 % triethylamine employing an injection volume of 1.0 mL. UV spectra were recorded and fractions analysed for collection at 215 nm with a Gilson 1 19 UV detector.
Data were integrated and reported using OpenLynx and OpenLynx Browser software or via Shimadzu PsiPort software. 1H NMR Spectroscopy: spectra were acquired on a either a Bruker DPX 250 MHz, a Bruker Avance III 400 MHz or a Bruker DRX 500 MHz spectrometer using residual protonated resonances of the deuterated solvent as the internal reference Preparation of Non Commercial Intermediates
3-(3-(ie^Butyldimethylsilyloxy)phenyl)-1 H^yrazolo[3,4-d]pyrimidin-4-amine (3)
Figure imgf000044_0001
To a stirred suspension of 3-iodo-1 /-/-pyrazolo[3,4-c/]pyrimidin-4-amine (1 ) (8.22 g, 31 .5 mmol), 3-phenol boronic acid (13.0 g, 94.5 mmol) and potassium phosphate (10.0 g, 47.3 mmol) in degassed DMF/water (3:2, 140 mL) was added [dppf] palladium (II) dichloride (13.0 g, 15.7 mmol). The reaction mixture was flushed with nitrogen, heated at 120 C for 2 hr and then allowed to cool to RT. The reaction mixture was diluted with EtOAc (500 mL) and aqueous hydrochloric acid (2 M, 500 mL) and the resulting suspension was filtered. The filtrate was extracted with aqueous hydrochloric acid (2 M, 2 x 500 mL). The combined aqueous extracts were basified with a saturated aq solution of sodium carbonate to pH 10. The precipitate formed was filtered and the filtrate was extracted with EtOAc (3 x 1 L). The combined organic extracts were dried, filtered and the solvent removed in vacuo to afford a grey solid. All solid materials generated during the workup procedure were combined and triturated with DCM to afford 3-(4- amino-1 /-/-pyrazolo[3,4-c/]pyrimidin-3-yl)phenol (2) (6.04 g, 84%) as a grey solid: m/z 228 (M+H)+ (ES+).
To a stirred solution of compound (2) (4.69 g, 20.66 mmol) and imidazole (2.10 g, 30.99 mmol) in dry DMF (100 mL) was added TBDMSCI (4.70 g, 30.99 mmol). After 16 hr, further aliquots of imidazole (2.10 g, 30.99 mmol) and TBDMSCI (4.70 g, 30.99 mmol) were added and the mixture was stirred for 48 hr. The reaction mixture was diluted with water (120 mL) and extracted with DCM (2 x 200 mL). The combined organic extracts were washed with water (2 x 200 mL), dried, filtered and the volume reduced to approximately 100 mL by evaporation in vacuo. The resulting slurry was filtered and the solid washed with heptane (50 mL) to afford the title compound (3) (6.05 g, 85%) as an off-white solid: m/z 343 (M+H)+ (ES+). 3-(4-(ferf-Butyldimethylsilyloxy)phenyl)-1 H-pyrazolo[3,4-d]pyrimidin-4-amine (5)
Figure imgf000045_0001
To a stirred suspension of 3-iodo-1 /-/-pyrazolo[3,4-c/]pyrimidin-4-amine (1 ) (10.0 g, 38.4 mmol), [dppf] palladium (II) dichloride (1 .37 g, 1 .92 mmol) and 4-phenol boronic acid (15.8 g, 1 15.2 mmol) in DMF (90 mL) was added aq sodium hydroxide (1 M, 76.0 mL, 76.8 mmol). The reaction was flushed with nitrogen and heated at 120 C for 18 hr. The reaction mixture was allowed to cool to RT and diluted with water (200 mL). The resulting precipitate was collected by filtration and washed with water (200 mL) and then DCM, until the filtrate ran colourless, to afford 4-(4-amino-1 H-pyrazolo[3,4-c/]pyrimidin-3-yl)phenol (4) (5.25 g, 60%) as a brown solid: m/z 228 (M+H)+ (ES+).
To a stirred solution of compound (4) (5.25 g, 23.1 mmol) and imidazole (3.85 g, 57.8 mmol) in dry DMF (70 mL) was added TBDMSCI (4.19 g, 27.7 mmol). After 16 hr, further aliquots of imidazole (1.93 g, 28.9 mmol) and TBDMSCI (2.10 g, 13.9 mmol) were added and the mixture stirred for 4 hr. The reaction mixture was diluted with water (200 mL) and the resulting precipitate was collected by filtration, washed with water (200 mL) and heptane (300 mL) to afford the title compound (5) (7.31 g, 93%) as a brown solid: m/z 344 (M+H)+ (ES+).
5-bromo-3-(2-chlorobenzyl)-2-(chloromethyl)quinazolin-4(3H)-one (8)
Figure imgf000045_0002
7a; x = ci
7b; X = OH
To a stirred solution of 2-amino-6-bromo-benzoic acid (6) (3.06 g, 14.2 mmol) in toluene (75 mL) cooled to 0°C in an ice-bath was added pyridine (0.60 mL, 7.10 mmol) followed by a solution of chloroacetyl chloride (2.26 mL, 28.4 mmol) in toluene (75 mL) drop-wise over 1 hr. The reaction mixture was allowed to warm to RT, and was heated at 1 15°C for 3 hr and then allowed to cool to RT. The solvent volume was reduced by half by evaporation in vacuo. Upon standing overnight, the product precipitated and was collected by filtration to afford 2-bromo-6- (2-chloroacetamido)benzoic acid (7a) (1.44 g) as a white solid: m/z 290/292 (M+H)+ (ES+). The filtrate was concentrated in vacuo and the residue triturated with ethanol/heptane to afford 2- bromo-6-(2-hydroxyacetamido) benzoic acid (7b) (1 .02 g, combined yield, 59%): m/z 274/276 (M+H)+ (ES+). Both 7a and 7b can be used without further purification in the next step.
To a stirred mixture of compound (7a) (7.50 g, 27.4 mmol), 2-chlorobenzylamine (5.00 mL, 41.05 mmol) and triethylamine (5.70 mL, 41 .1 mmol) in toluene (250 mL) was added a solution of phosphorus trichloride (2.60 mL, 30.1 mmol) in toluene (250 mL) dropwise over 1 hr. The reaction mixture was heated to 1 10°C for 24 hr, whereupon the hot solution was decanted and concentrated in vacuo. The residue was triturated with propan-2-ol (50 mL) to afford the title compound (8) (6.41 g, 59%) as a yellow solid: m/z 397/399 (M+H)+ (ES+).
3-(2-(2-Methoxyethoxy)ethoxy)prop-1 -yne (9)
Figure imgf000046_0001
9
To a stirred suspension of sodium hydride (3.12 g, 78.0 mmol) in THF (100 mL) cooled to 5°C in an ice-bath was added a solution of propargyl alcohol (4.60 g, 78.0 mmol) in THF (20 mL) drop-wise. The mixture was stirred at 5°C for 30 min and was then allowed to warm to RT and treated dropwise with 1 -bromo-2-(2-methoxyethoxy)ethane (7.00 mL, 52.0 mmol). The reaction mixture was stirred at RT for 16 hr and was then, diluted with water (100 mL) and extracted with diethyl ether (2 x 100 mL). The combined organic extracts were dried, filtered and evaporated in vacuo. The residue was purified by flash column chromatography, eluting with 30% EtOAc in heptane, to afford the title compound (9) (250 mg, 9%) as a colourless oil: 1H NMR (500 MHz, MeOD) δ : 4.18 (2H, d), 3.66-3.69 (2H, m), 3.60-3.66 (4H, m), 3.52-3.56 (2H, m), 3.36 (3H, s), 2.84 (1 H, t). 2-(2-(Prop-2-ynyloxy)ethoxy)ethanol (10)
Figure imgf000046_0002
10
To a stirred suspension of potassium ie f-butoxide (1 .06 g, 9.42 mmol) in THF (300 mL) cooled to 0°C in an ice-bath was added a solution of 2,2'-oxydiethanol (4.60 g, 78.0 mmol) in THF (10 mL). The mixture was stirred at 0°C for 30 min and was then allowed to warm to RT and was treated dropwise with propargyl bromide (1.68 mL of an 80% solution in toluene, 1 1 .30 mmol). The reaction mixture was stirred at RT for 16 hr and diluted with brine and water (5:1 , 50 mL) and was extracted with EtOAc (3 x 500 mL). The combined organic extracts were dried, filtered and evaporated in vacuo. The residue was purified by flash column chromatography, eluting with 25 to 60% EtOAc in heptane, to afford the title compound (10) (410 mg, 30%) as a yellow oil: 1H NMR (250 MHz, CDCI3) δ: 2.22 (1 H, s), 2.45 (1 H, t), 3.58-3.66 (2H, m), 3.67-3.79 (6H, m), 4.22 (2H, d).
1 -Morpholinohex-5-yn-1 -one (11 )
Figure imgf000047_0001
To a solution of hex-5-ynoic acid (500 mg, 4.46 mmol), DMAP (27 mg, 0.23 mmol) and EDC hydrochloride (940 mg, 4.90 mmol) in DCM (10 mL) was added morpholine (391 μΙ_, 4.46 mmol). The reaction was stirred at RT overnight and then diluted with DCM (50 mL). The resulting solution was washed with water (3 x 10 mL), brine (10 mL) and aq hydrochloric acid (2 M, 2 x 20 mL) and was then dried, filtered and evaporated in vacuo to afford the title compound (11 ) (808 mg, 100%) as a yellow oil: 1H NMR (500 MHz, CDCI3) δ : 3.65-3.71 (4H, m), 3.63 (2H, d), 3.45-3.54 (2H, m), 2.46 (2H, t), 2.26-2.33 (2H, m), 1 .98 (1 H, t), 1.88 (2H, quin).
2-Acetamido-/V-(prop-2-ynyl)acetamide (12)
Figure imgf000047_0002
To a solution of /V-acetylglycine (200 mg, 1 .71 mmol), DMAP (1 1 mg, 0.09 mmol) and EDC hydrochloride (360 mg, 1 .88 mmol) in DCM (10 mL) was added propargylamine (109 μί, 1 .71 mmol). The reaction mixture was stirred at RT overnight and then evaporated in vacuo. The residue was purified by flash column chromatography, eluting with 0 to 5% methanol in DCM, to afford the title compound (12) (218 mg, 83%) as a white solid: 1H NMR (500 MHz, CDCI3) δ : 2.07 (3H, s), 2.25 (1 H, t), 3.95 (2H, d), 4.07 (2H, dd), 6.34 (1 H, br s), 6.49 (1 H, b s).
W-(But-3-ynyl)morpholine-4-carboxamide (13)
Figure imgf000047_0003
To a suspension of but-3-yn-1 -amine hydrochloride (200 mg, 1 .89 mmol) and triethylamine (425 μί, 3.78 mmol) in DCM (60 mL) was added morpholine-4-carbonyl chloride (218 μ L, 1 .89 mmol). The reaction was stirred at RT overnight and then washed with aq hydrochloric acid (2 M, 2 x 20 mL), saturated aq sodium bicarbonate (2 x 20 mL), dried, filtered and evaporated in vacuo. The residue was purified by flash column chromatography, eluting with 0-100% ethyl acetate in heptane, to afford the title compound (13) (164 mg, 48%) as a white solid. 1H NMR (500 MHz, DMSO-de) δ : 2.28 (2H, td), 2.82 (1 H, t), 3.09-3.18 (2H, m), 3.20-3.27 (4H, m), 3.48- 3.57 (4H, m), 6.74 (1 H, t).
(2-Fluoro-3-methoxyphenyl)methanamine (15)
Figure imgf000048_0001
To a stirred solution of sodium hydrogen carbonate (2.73 g, 32.5 mmol) in water (50 mL) was added hydroxylamine hydrochloride (2.30 g, 33.2 mmol) in portions over 30 min. The resultant solution was added to a vigorously stirred suspension of 2-fluoro-3-methoxybenzaldehyde (5.00 g, 32.5 mmol) in ethanol (45 mL) and the reaction mixture stirred at RT for 16 hr. The resultant precipitate was removed by filtration and washed with water (3 x 100 mL) and then allowed to dry in air to afford 2-fluoro-3-methoxybenzaldehyde oxime (14) (4.69 g, 85%) as a white crystalline solid: m/z 170 (M+H)+ (ES+).
To a stirred solution of the oxime (14) (1 .50 g, 8.86 mmol) in ethanol (70 mL) under nitrogen was added 10% palladium on carbon (100 mg). The reaction mixture was placed under an atmosphere of hydrogen and stirred at RT for 2 hr. The reaction mixture was filtered through Celite™, and was then evaporated in vacuo to remove the ethanol. The resultant crude residue was partitioned between ethyl acetate (50 mL) and aq hydrochloric acid (2 M, 50 mL). The aq layer was separated and was neutralised with saturated sodium hydrogen carbonate solution and then re-extracted with ethyl acetate (70 mL) followed by a mixture of 2-propanol and chloroform (1 :1 , 2 x 70 mL). The propanol/chloroform extracts were dried, filtered and evaporated in vacuo separately to afford the title compound (15) in two batches (batch 1 : 346 mg, 25%; batch 2: 370 mg, 27%) as white solids: m/z 156 (M+H)+ (ES+) (method C).
2-((4-amino-3-iodo-1 H^yrazolo[3,4-d]pyrimidin-1 -yl)methyl)-5-bromo-3-(2-chloro benzyl)quinazolin-4(3H)-one: Intermediate A 8 = Intermediate A
Figure imgf000049_0001
To a stirred mixture of 5-bromo-3-(2-chlorobenzyl)-2-(chloromethyl)quinazolin-4(3/-/)-one, (8), (13.6 g, 30.7 mmol) and 3-iodo-1 H-pyrazolo[3,4-d]pyrimidin-4-amine (1 ) (8.09 g, 30.7 mmol) in DMF (300 mL was added potassium carbonate (6.36 g, 46.0 mmol) and the reaction maintained at RT in the dark for 24 hr. The mixture was poured onto water (4.0 L) and the resulting suspension was stirred at RT for 1 hr. The precipitate was isolated by filtration and dried in vacuo to afford the title compound, Intermediate A, as a colourless solid (18.0 g, 94%); m/z 622/624 [M+H]+ (ES+). 2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-d]pyrimidin-1 -yl)methyl)-5-bromo-3-(2- chlorobenzyl)quinazolin-4(3H)-one: Intermediate B
Intermediate B
Figure imgf000049_0002
To a stirred mixture of compound (8) (100 mg, 0.25 mmol) and potassium carbonate (42 mg, 0.30 mmol) in DMF (2.5 mL) was added a solution of compound (3) (94 mg, 0.28 mmol) in DMF (2.5mL) and the reaction mixture was stirred at RT for 18 hr. Potassium carbonate (3 x 35 mg, 0.75 mmol) was added in three portions over 30 hr. The solvent was removed in vacuo and the crude material was purified by flash column chromatography, eluting with 4.5% methanol in DCM, to afford the title compound (Intermediate B) (94 mg, 64%) as a off-white solid: m/z 588/590 (M+H)+ (ES+) (Method B).
2-((4-Amino-3-(4-hydroxyphenyl)-1 H^yrazolo[3,4-d]pyrimidin-1 -yl)methyl)-5-bromo-3-(2- chlorobenzyl)quinazolin-4(3H)-one: Intermediate C = Intermediate C
Figure imgf000050_0001
Route A:
A thoroughly ground mixture of Intermediate A (10.0 g, 15.3 mmol), Na2CO3.10H2O (9.60 g, 33.6 mmol), 4-hydroxyphenylboronic acid (4.21 g, 30.5 mmol), and Pd(Ph3)4 (1.32 g, 1.14 mmol) in EtOH (300 mL) was purged with nitrogen and was then stirred at 85°C for 3 days. The mixture was cooled to RT and the precipitate was isolated by filtration and was washed with EtOH (100 mL). The solid was resuspended in water (100 mL) and was stirred for a further 30 min was then isolated by filtration, washed with EtOH (50 mL) and dried in vacuo to afford the title compound, Intermediate C as an off white solid (4.86 g, 51 %); m/z 588/590 (M+H)+ (ES+).
Route B:
To a stirred mixture of compound (8) (100 mg, 0.25 mmol) and potassium carbonate (42 mg, 0.30 mmol) in DMF (2.5 mL) was added a solution of compound (5) (94 mg, 0.28 mmol) in DMF (2.5 mL) and the reaction mixture stirred at RT for 18 hr. The solvent was removed in vacuo and the crude material was purified by flash column chromatography, eluting with 4.5% methanol in DCM, to afford the title compound, Intermediate C (82 mg, 55%) as a cream solid: m/z 588/590 (M+H)+ (ES+) (Method B).
2-((4-Amino-3-(3-fluoro-4-hydroxyphenyl)-1 H^yrazolo[3,4-d]pyrimidin-1 -yl)methyl)-5- bromo-3-(2-chlorobenzyl)quinazolin-4(3H)-one: Intermediate D.
8 = Intermediate D To a stirred mixture of compound (8) (200 mg, 0.502 mmol) and potassium carbonate (83 mg, 0.60 mmol) in DMF (2.0 mL) was added a solution of 4-(4-amino-1 H-pyrazolo[3,4-d]pyrimidin-3- yl)-2-fluorophenol (see Table 1 , below) (148 mg, 0.602 mmol) in DMF (2.0 mL) and the reaction mixture stirred at RT for 18 hr. The solvent was removed in vacuo and the crude material was purified by flash column chromatography, eluting with 5% methanol in DCM, to afford the title compound, Intermediate D (81 mg, 27%) as a off-white solid: m/z 606/608 (M+H)+ (ES+) (Method B).
2-((4-Amino-3-(4-hydroxy-3-methoxyphenyl)-1 H^yrazolo[3,4-cf]pyrimidin-1 -yl)methyl)-5- bromo-3-(2-chlorobenzyl)quinazolin-4(3H)-one: Intermediate E.
Intermediate A
= Intermediate E
Figure imgf000051_0001
A mixture of Intermediate A (400 mg, 0.630 mmol), 2-methoxy-4-(4,4,5,5-tetramethyl-1 ,3,2- dioxaborolan-2-yl)phenol (189 mg, 0.755 mmol), Na2CO3.10H2O (396 mg, 1.39 mmol) and Pd(Ph3)4 (55 mg, 0.047mmol) in EtOH (12 mL) was purged with nitrogen and was then stirred at 85°C for 24 hr. The mixture was cooled and was evaporated in vacuo and the residue was purified by flash column chromatography (Si02, 80 g, MeOH in CH2CI2, 0-10 %, gradient elution) to afford the title compound, Intermediate E, as a pale brown solid (225 mg, 56%); m/z 618/620 (M+H)+ (ES+).
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-d]pyrimidin-1 -yl)methyl)-3-(2- chlorobenzyl)-5-(5-iodopent-1 -ynyl)quinazolin-4(3H)-one: Intermediate F.
Figure imgf000051_0002
To a mixture of Intermediate C (503 mg, 0.854 mmol), copper(l) iodide (17 mg, 0.089 mmol) and bis(triphenylphosphine)palladium(ll) dichloride (64 mg, 0.089 mmol) in diethylamine (3.2 mL, 31 mmol) was added pent-4-yn-1 -ol (157 μΙ_, 1 .70 mmol). The reaction mixture was degassed with N2 and was heated at 60°C for 2.5 hr and then cooled to RT. The resulting mixture was evaporated in vacuo and the residue was triturated with EtOAc (5.0 mL) and dried in vacuo to afford 2-((4-amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-d]pyrimidin-1 -yl)methyl)-3-(2- chlorobenzyl)-5-(5-hydroxypent-1 -ynyl)quinazolin-4(3H)-one (16) as a brown solid (488 mg, 92% pure, 89%); m/z 590/592 (M+H)+ (ES+) (Method D). To a stirred suspension of the alcohol (16) (162 mg, 92% pure, 0.252 mmol) in DCM (8.0 mL) was added iodine (121 mg, 0.477 mmol), triphenylphosphine (1 15 mg, 0.438 mmol) and imidazole (35 mg, 0.51 mmol) and the mixture maintained at RT for 2 hr. The reaction mixture was evaporated in vacuo and the residue was purified by flash column chromatography (Si02, 40 g, MeOH in DCM, 0-10%, gradient elution) to afford Intermediate F (109 mg, 73% pure, 45.0 %) as a yellow solid m/z 702/704 (M+H)+ (ES+) (Method D), which was used without further purification in subsequent transformations.
Additonal intermediates and non-commericial building blocks, that appear in the reaction schemes which follow, were prepared using the procedures described above for analogous derivatives or by the methods described in the cited literature references (Table 1 ).
Table 1 : Non Commercial Compounds
Literature Citation or
Structure Compound Name
Analogous Procedure
Figure imgf000052_0001
Figure imgf000053_0001
Gautier et al., Annates Pharmaceutiques line
Francaises, 1971 , 29, 39-50
Prepared using the procedure for compound (9) )prop-1 -yne
starting from 2-bromoethyl methyl ether
Prepared using the hex-5- procedure for compound
(11 ) substituting
2-methoxyethanamine
Prepared using the
-yn-1 -one procedure for compound
(11 ) from hept-6-ynoic acid
Prepared using the
-yn-1 -one procedure for compound
(11 ) from pent-4-ynoic acid
Prepared using the
-5-yn-1 -one procedure for compound
(11 ) substituting piperidine
Figure imgf000053_0002
Prepared using the
NEt, procedure for compound
/V,/V-diethylhex-5-ynamide
(11 ) substituting
diethylamine
Prepared using the
1 -(4-methylpiperazin procedure for compound
Me en-1 -one (11 ) substituting 4-methyl piperazine
Prepared using the
1 -(4-morpholinopiperidin-1 - procedure for compound yl)hex-5-yn-1 -one (11 ) substituting 4- morpholinopiperidine
Figure imgf000053_0003
Prepared using the
1 -(4-acetylpiperazin-1 -yl)hex-5- procedure for compound yn-1 -one (11 ) substituting 4-acetyl piperazine Prepared using the
/V-(2-morpholinoethyl)hex-5- procedure for compound ynamide (11 ) substituting 2- morpholinoethanamine Prepared using the
/V-(tetrahydro-2H-pyran-4-yl)hex- procedure for compound 5-ynamide (11 ) substituting tetrahydro-
2/-/-pyran-4-amine
Prepared using the
/V-(2-(dimethylamino)ethyl)hex- procedure for compound 5-ynamide (11 ) substituting N,N- dimethylethane-1 ,2-diamine Prepared using the fe/f-butyl 4-(hex-5- procedure for compound ynamidomethyl)piperidine-1 - (11 ) substituting fe/f-butyl 4- carboxylate (aminomethyl)piperidine-l - carboxylate
Prepared using the
(R)-1 -(3-(dimethylamino) procedure for compound pyrrolidin-1 -yl)hex-5-yn-1 -one (11 ) substituting {R)-N,N-
Figure imgf000054_0001
dimethylpyrrolidin-3-amine
Figure imgf000054_0002
Prepared using the
1 -(4-methyl-1 ,4-diazepan-1 - procedure for compound yl)hex-5-yn-1 -one (11 ) substituting 1 -methyl-
1 ,4-diazepane
Prepared using the
1 -(4-(dimethylamino)piperidin-1 - procedure for compound yl)hex-5-yn-1 -one (11 ) substituting 4-dimethyl
Figure imgf000054_0003
aminopiperidine Prepared using the
1 -(4-(2-methoxyethyl)piperazin- procedure for compound 1 -yl)hex-5-yn-1 -one (11 ) substituting 1 -(2-
Figure imgf000055_0001
methoxyethyl)piperazine -
yl -2-
Figure imgf000055_0002
ethanamine
Prepared using the methodology employed to
3-((5-bromo-2-(chloromethyl)-4- generate compound (8) oxoquinazolin-3(4/-/)-yl)methyl)
substituting 3-cyano benzonitrile, Compound (17)
benzylamine hydrochloride in the second step
3- ((2-((4-amino-3-(3-hydroxy
Prepared according to the phenyl)-1 /-/-pyrazolo[3,4-c/]
methodology used to pyrimidin-1 -yl)methyl)-5-bromo- generate Intermediate B,
4- oxoquinazolin-3(4/- )-yl)methyl)
substituting Compound (17) benzonitrile; Compound (18)
Figure imgf000055_0003
COMPOUND EXAMPLES Example 1 : 2-((4-Amino-3-(3-hydroxyphenyl)-1 H^yrazolo[3,4-rf]pyrimidin-1 -yl)methyl)-3- (2-chlorobenzyl)-5-ethynylquinazolin-4(3H)-one. Intermediate A
Figure imgf000056_0001
19 Example 1
To a mixture of Intermediate A (91 mg, 0.15 mmol), (triisopropylsilyl)acetylene (87 μ L, 0.39 mmol), copper (I) iodide (1.8 mg, 0.01 mmol) and diethylamine (242 μ L, 2.31 mmol) in degassed DMF (2.0 mL) was added t»/s(triphenylphosphine)palladium (II) dichloride (6.5 mg, 0.01 mmol). The reaction mixture was purged with nitrogen, sealed and then heated under microwave irradiation (120 C, 200 W, CEM: Discover microwave) for 20 min. The mixture was cooled to RT, diluted with EtOAc (3 mL) and washed with water (1 mL). The aq phase was back extracted with EtOAc (1 .0 mL) and the combined organic extracts were washed with water (1 .0 mL). The aq phase was back extracted with EtOAc (1 .0 mL) and the combined organic extracts were washed with water (1 .0 mL). The aq phase was back extracted with EtOAc (1 .0 mL) and the combined organic extracts were washed with a saturated solution of aq ammonium chloride (2 x 2.0 mL) and then dried, filtered and evaporated in vacuo. The crude material was triturated with methanol (1 .0 mL) and the resulting solid was purified by flash column chromatography, eluting with 0 to 50% methanol in DCM, to afford 2-((4-amino-3-(3- hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chlorobenzyl)-5-
((triisopropylsilyl)ethynyl)quinazolin-4(3/-/)-one (19) (107 mg, 100%) as a white solid: m/z 690/692 (M+H)+ (ES+).
To a stirred solution of compound (19) (107 mg, 0.15 mmol) in DMF (0.50 mL) was added tetrabutylammonium fluoride (190 μ L of a 1 .0 M solution in THF, 0.19 mmol). After 10 min, the reaction mixture was diluted with water (3.0 mL) and cooled to 0°C for 30 min. The resulting solid was collected by filtration and purified by flash column chromatography, eluting with 4.5% methanol in DCM, to afford the title compound, Example 1 , (70 mg, 85%) as a white solid: m/z 534/536 (M+H)+ (ES+); Rl 3.73 min; 1H NMR (500 MHz, DMSO-d6) δ: 4.42 (1 H, s), 5.30 (2H, s), 5.77 (2H, s), 6.16 (1 H, d), 6.79 (1 H, t), 6.84 (1 H, dd), 6.89-6.96 (2H, m), 7.04 (1 H, t), 7.14 (1 H, d), 7.30 (1 H, t), 7.70 (1 H, d), 7.75 (1 H, d), 7.80-7.87 (1 H, m), 8.18 (1 H, s), 9.67 (1 H, s).
Example 2: 2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-d]pyrimidin-1 -yl)methyl)-3- (2-chlorobenzyl)-5-(3-(2-(2-methoxyethoxy)ethoxy)prop-1 -yn-1 -yl)quinazolin-4(3H)-one. Intermediate A
Figure imgf000057_0001
Example 2
To a mixture of Intermediate A (50 mg, 84.9 μηηοΙ), the acetylene (9) (20 mg, 0.13 mmol), copper iodide (1.0 mg, 5.09 μ mol) and diethylamine (133 μ L, 1 .27 mmol) in degassed DMF (1 .0 mL) was added t)/s(triphenylphosphine)palladium(ll) dichloride (3.6 mg, 5.09 μηηοΙ). The reaction mixture was purged with nitrogen, sealed and then heated under microwave irradiation (120 C, 200 W, CEM: Discover microwave) for 20 min. The mixture was cooled to RT and was diluted with water (10 mL) and extracted with EtOAc (2 x 20 mL). The combined organic extracts were dried, filtered and evaporated in vacuo. The residue was purified by flash column chromatography, eluting with 0-8% MeOH in DCM, to afford the title compound, Example 2, (21 mg, 36%) as an orange solid: m/z 667 (M+H)+ (ES+); Rl 3.68 min; 1H NMR (500 MHz, DMSO-de) δ : 3.19 (3H, s), 3.37 (2H, dd), 3.46 (2H, dd), 3.48-3.52 (2H, m), 3.59-3.64 (2H, m), 4.38 (2H, s), 5.30 (2H, s), 5.76 (2H, s), 6.16 (1 H, d), 6.76-6.81 (1 H, m), 6.84 (1 H, dd), 6.89-6.95 (2H, m), 7.01 -7.07 (1 H, m), 7.13 (1 H, d), 7.30 (1 H, t), 7.67 (1 H, d), 7.73 (1 H, d), 7.84 (1 H, t), 8.17 (1 H, s), 9.66 (1 H, s).
Example 3: 2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-d]pyrimidin-1 -yl)methyl)-3- (2-chlorobenzyl)-5-(6-morpholino-6-oxohex-1 -yn-1 -yl)quinazolin-4(3H)-one.
Intermediate A
Figure imgf000057_0002
Example 3
To a mixture of Intermediate A (50 mg, 85 μ mol), compound (11 ) (38 mg, 212 μηιοΙ), copper iodide (1 .0 mg, 5.1 μ mol) and diethylamine (133 μί, 1 .27 mmol) in degassed DMF (2.0 mL) was added t»/s(triphenylphosphine)palladium(ll) dichloride (4.0 mg, 5.1 μηηοΙ). The reaction mixture was purged with nitrogen, sealed and then heated under microwave irradiation (120°C, 200 W, CEM: Discover microwave) for 20 min. The reaction mixture was concentrated and the residue was purified by flash column chromatography, eluting with 0-50% methanol in DCM, to afford the title compound, Example 3, (31 mg, 53%) as a white solid: m/z 689/691 (M+H)+ (ES+), R' 3.63 min; 1H NMR (500 MHz, DMSO-d6) δ: 1 .66-1.76 (2H, m), 2.45-2.55 (4H, m), 3.19-3.26 (2H, m), 3.28-3.36 (4H, m), 3.41 -3.47 (2H, m), 5.29 (2H, s), 5.75 (2H, s), 6.14 (1 H, d), 6.78 (1 H, t), 6.84 (1 H, d) 6.89-6.94 (2H, m) 7.03 (1 H, t) 7.1 1 (1 H, d) 7.26-7.33 (1 H, m) 7.60 (1 H, d) 7.68 (1 H, d), 7.77-7.83 (1 H, m), 8.18 (1 H, s), 9.68 (1 H, s).
Example 4: 6-(2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-d]pyrimidin-1 -yl) methyl)-3-(2-chlorobenzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)hex-5-ynoic acid.
Intermediate C
Figure imgf000058_0001
Example 4
To a mixture of Intermediate C (150 mg, 0.25 mmol), hex-5-ynoic acid (69 μ L, 0.63 mmol), copper iodide (3.0 mg, 0.02 mmol) and diethylamine (400 μΙ_, 3.75 mmol) in degassed DMF (1 .5 mL) was added t»/s(triphenylphosphine)palladium(ll) dichloride (1 1.0 mg, 0.02 mmol). The reaction mixture was purged with nitrogen, sealed and then heated under microwave irradiation (120 C, 200 W, CEM: Discover microwave) for 20 min. The mixture was cooled to RT, diluted with water (2.0 mL) and EtOAc (2.0 mL) and neutralised with 1 M aq hydrochloric acid to pH 7. The suspension was concentrated and purified by flash column chromatography, eluting with 0- 50% methanol in DCM to afford the title compound, Example 4, (22 mg, 14%) as a yellow solid: m/z 620/622 (M+H)+ (ES+); Rl 3.47 min; 1H NMR (500 MHz, DMSO-d6) δ: 1 .68-1 .78 (2H, m), 2.29-2.41 (2H, m), 2.42-2.47 (2H, m), 5.29 (2H, s), 5.72 (2H, s), 6.19 (1 H, d), 6.79 (1 H, t), 6.88 (2H, d), 7.03 (1 H, t), 7.12 (1 H, d), 7.32 (2H, d), 7.60 (1 H, d), 7.66 (1 H, d), 7.78 (1 H, t), 8.16 (1 H, s), 9.73 (1 H, s), 12.04 (1 H, s).
Example 5: 2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-d]pyrimidin-1 -yl)methyl)-3- (2-chlorobenzyl)-5-(6-morpholino-6-oxohex-1 -yn-1 -yl)quinazolin-4(3H)-one. Example 4
Figure imgf000059_0001
To a solution of Example 4 (16.0 mg, 26 μηηοΙ), 4-dimethylaminopyridine (0.6 mg, 5.2 μηηοΙ), and EDC hydrochloride (5.3 mg, 28 μηηοΙ) in DMF (0.5 mL) was added morpholine (2.3 μ L, 26 μηηοΙ). The reaction mixture was stirred at RT overnight and then diluted with water (5.0 mL). The resulting solid was collected by filtration and purified by flash column chromatography, eluting with 0-7% methanol in DCM to afford the title compound, Example 5, (7.0 mg, 57%) as a yellow solid: m/z 689/691 (M+H)+ (ES+); Rl 3.54 min; 1H NMR (500 MHz, DMSO-d6) δ: 1.71 (2H, quin), 2.43-2.49 (2H, m), 2.52-2.55 (2H, m), 3.20-3.26 (2H, m), 3.28-3.33 (4H, m), 3.44 (2H, t), 5.28 (2H, s), 5.72 (2H, s), 6.17 (1 H, d), 6.78 (1 H, t), 6.87 (2H, d), 7.02 (1 H, t), 7.10 (1 H, d), 7.32 (2H, d), 7.60 (1 H, d), 7.67 (1 H, d), 7.79 (1 H, t), 8.16 (1 H, s), 9.75 (1 H, s).
Example 6: 3-((2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-cf]pyrimidin-1 methyl)-5-(3-(2-(2-hydroxyethoxy)ethoxy)prop-1 -yn-1 -yl)-4-oxoquinazolin-3(4H)- yl)methyl)benzonitrile.
Figure imgf000059_0002
18 Example 6
To a mixture of the aryl bromide (18), (see Table 1 , above) (50 mg, 86 μηιοΙ), the acetylene (10) (31 mg, 216 μηηοΙ), copper iodide (1 .0 mg, 5.1 μηηοΙ) and diethylamine (135 μί, 1.29 mmol) in degassed DMF (1 .0 mL) was added t»/s(triphenylphosphine)palladium(ll) dichloride (3.6 mg, 5.2 μηηοΙ). The reaction mixture was purged with nitrogen, sealed and then heated in the microwave (120°C, 200 W, CEM: Discover microwave) for 20 min. The reaction mixture was concentrated and the residue was purified by flash column chromatography, eluting with 5.5% methanol in DCM, to afford the title compound, Example 6, (21 mg, 38%) as a white solid: m/z 643 (M+H)+ (ES+), Rt (min) 3.22; 1H NMR (500 MHz, DMSO-d6) δ: 3.37-3.40 (2H, m), 3.41 -3.47 (2H, m), 3.49-3.55 (2H, m), 3.62-3.67 (2H, m), 4.40 (2H, s), 4.59 (1 H, t), 5.37 (2H, s), 5.74 (2H, s), 6.81 -6.86 (1 H, m), 6.89-6.97 (3H, m), 7.18 (1 H, t), 7.22 (1 H, s), 7.29 (1 H, t), 7.51 (1 H, d), 7.63-7.68 (2H, m), 7.77-7.83 (1 H, m), 8.27 (1 H, br s), 9.68 (1 H, s).
Example 7: 2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-d]pyrimidin-1 -yl) methyl)-3-(2-chlorobenzyl)-5-(5-(cyclopentylamino)pent-1 -ynyl)quinazolin-4(3H)-one.
Intermediate F
Figure imgf000060_0001
Example 7
To a suspension of Intermediate F (100 mg, 73% pure, 0.142 mmol) in DCM (10 mL) at RT was added cyclopentanamine (0.100 mL, 1 .01 mmol). The suspension rapidly dissolved and the resulting solution was stirred at RT for 3 days then evaporated in vacuo. The residue was purified by flash column chromatography (Si02, 12 g, MeOH in EtOAc, 0-100 %, gradient elution) and the crude product so obtained was triturated with MeCN to afford Example 7 as a yellow solid (16 mg, 17%); Rl 1.39 min; m/z 659/661 (M+H)+ (ES+) (Method D); 1H NMR (400 MHz, DMSO-de) δ: 1 .22 (2H, m), 1.38 (2H, m), 1.50-1 .64 (6H, overlapping m), 2.46 (2H, t), 2.60 (2H, t), 2.89 (1 H, quin), 5.29 (2H, s), 5.73 (2H, s), 6.15 (1 H, d), 6.77 (1 H, td), 6.87 (2H, d), 7.02 (1 H, m), 7.1 1 (1 H, dd), 7.32 (2H, d), 7.58 (1 H, dd), 7.67 (1 H, dd), 7.78 (1 H, m), 8.16 (1 H, s), 9.77 (1 H, br s).
Additional examples, set out below (Table 2), were prepared utilising the same synthetic methodologies as those disclosed above.
Table 2
Example
Structure Name and Analytical Data
No. 3
,
Figure imgf000061_0001
2-((4-Amino-3-(3-hydroxyphenyl)-1/-/-pyrazolo[3,4- c/]pyrimidin-1-yl)methyl)-3-(3-chlorobenzyl)-5-ethynyl quinazolin-4(3/-/)-one.
R' 3.73 min; m/z 534/536 (M+H)+ (ES+);
10 1H NMR (500 MHz, DMSO-d6) δ : 4.45 (1 H, s), 5.36
(2H, s), 5.75 (2H, s), 6.70 (1H, d), 6.81-6.87 (2H, m),
6.92-6.98 (2H, m), 7.07 (1H, t), 7.11-7.19 (1H, m),
7.30 (1H, t), 7.61 (1H, d), 7.66-7.70 (1H, m), 7.75-7.81
(1H, m), 8.23 (1H, s), 9.66 (1H, s).
2-((4-Amino-3-(4-hydroxyphenyl)-1/-/-pyrazolo[3,4- c/]pyrimidin-1-yl)methyl)-3-(3-chlorobenzyl)-5-ethynyl quinazolin-4(3/-/)-one.
R' 3.65 min; m/z 534/536 (M+H)+ (ES+);
11 1H NMR (500 MHz, DMSO-d6) δ : 4.44 (1 H, s), 5.35
(2H, s), 5.71 (2H, s), 6.69 (1H, d), 6.82 (1H, s), 6.87
(2H, d), 7.06 (1H, t), 7.11-7.17 (1H, m), 7.33 (2H, d),
7.58-7.64 (1H, m), 7.68 (1H, d), 7.75-7.82 (1H, m),
8.21 (1H, s), 9.80 (1H, br s).
2-((4-Amino-3-(3-hydroxyphenyl)-1/-/-pyrazolo[3,4- c/]pyrimidin-1-yl)methyl)-5-ethynyl-3-(2-fluorobenzyl) quinazolin-4(3/-/)-one.
R' 3.63 min; m/z 518 (M+H)+ (ES+);
12 1H NMR (500 MHz, DMSO-d6) δ : 4.42 (1 H, s), 5.35
(2H, s), 5.78 (2H, s), 6.35 (1H, t), 6.74 (1H, t), 6.81-
6.87 (1H, m), 6.87-6.97 (3H, m), 7.10 (1H, q), 7.21-
7.36 (1H, m), 7.62-7.73 (2H, m), 7.76-7.85 (1H, m),
Figure imgf000061_0002
8.19 (1H, s), 9.67 (1H, s). 2-((4-Amino-3-(4-hydroxyphenyl)-1/-/-pyrazolo[3,4- c/]pyrimidin-1-yl)methyl)-5-ethynyl-3-(2-fluorobenzyl) quinazolin-4(3/-/)-one.
R' 3.54 min; m/z 518 (M+H)+ (ES+);
1H NMR (500 MHz, DMSO-d6) δ : 4.42 (1 H, s), 5.34
(2H, s), 5.76 (2H, s), 6.35 (1H, t), 6.72 (1H, t), 6.84-
6.93 (3H, m), 7.09 (1H, q), 7.33 (2H, d), 7.64-7.74
(2H, m), 7.76-7.85 (1H, m), 8.17 (1H, s), 9.76 (1H, s)
2-((4-Amino-3-(4-hydroxyphenyl)-1/-/-pyrazolo[3,4- c/]pyrimidin-1-yl)methyl)-5-ethynyl-3-(3-methoxy benzyl )quinazolin-4(3/-/)-one.
R' 3.37 min; m/z 530 (M+H)+ (ES+);
1H NMR (500 MHz, DMSO-d6) δ : 3.60 (3H, s), 4.44
(1H, s), 5.35 (2H, s), 5.70 (2H, s), 6.34 (1H, d), 6.40
(1H, s), 6.84-6.91 (1H, m), 6.88 (2H, m), 7.00 (1H, t),
7.31-7.38 (2H, m), 7.55 (1H, d), 7.66 (1H, d), 7.75
(1H,t), 8.20 (1H,s), 9.75(1 H,s).
2-((4-Amino-3-(3-hydroxyphenyl)-1/-/-pyrazolo[3,4- c/]pyrimidin-1-yl)methyl)-5-ethynyl-3-(3-methoxy benzyl )quinazolin-4(3/-/)-one.
R' 3.48 min; m/z 530 (M+H)+ (ES+);
1H NMR (500 MHz, DMSO-d6) δ : 3.61 (3H, s), 4.44
(1H, s), 5.36 (2H, s), 5.73 (2H, s), 6.34 (1H, d), 6.40
(1H, s), 6.68 (1H, dd), 6.82-6.86 (1H, m), 6.93-6.97
(2H, m), 7.00 (1H, t), 7.31 (1H, t), 7.55 (1H, d), 7.67
Figure imgf000062_0001
(1H, d), 7.76 (1H, t), 8.22 (1H, s), 9.67 (1H, s).
7-
Figure imgf000062_0002
2-((4-Amino-3-(3-hydroxyphenyl)-1/-/-pyrazolo[3,4- c/]pyrimidin-1-yl)methyl)-5-ethynyl-3-(3-
(trifluoromethyl)benzyl)quinazolin-4(3/-/)-one.
R' 3.76 min; m/z 568 (M+H)+ (ES+);
17 1H NMR (500 MHz, DMSO-d6) δ : 4.44 (1 H, s), 5.45
(2H, s), 5.75 (2H, s), 6.82-6.86 (1H, m), 6.89-6.94
(3H, m), 7.22-7.27 (2H, m), 7.30 (1H, t), 7.44 (1H, d),
7.63 (1H, d), 7.68 (1H, d), 7.79 (1H, t), 8.20 (1H, s),
Figure imgf000062_0003
9.67 (1H, s). 2-((4-Amino-3-(4-hydroxyphenyl)-1 /-/-pyrazolo[3,4- c/]pyrimidin-1 -yl)methyl)-3-(4-chlorobenzyl)-5-ethynyl quinazolin-4(3/-/)-one.
R' 3.56 min; m/z 534/536 (M+H)+ (ES+);
1H NMR (500 MHz, DMSO-d6) δ : 4.44 (1 H, s), 5.32
(2H, s), 5.70 (2H, s), 6.73 (2H, d), 6.88 (2H, d), 7.05
(2H, d), 7.33 (2H, d), 7.62 (1 H, d), 7.68 (1 H, d), 7.75-
7.84 (1 H, m), 8.21 (1 H, s), 9.78 (1 H, br s).
2-((4-Amino-3-(3-hydroxyphenyl)-1 /-/-pyrazolo[3,4- c/]pyrimidin-1 -yl)methyl)-5-ethynyl-3-(4-
(methylsulfonyl)benzyl)quinazolin-4(3/-/)-one.
R' 3.27 min; m/z 578 (M+H)+ (ES+);
1H NMR (500 MHz, DMSO-d6) δ : 2.99 (3H, s), 4.43
(1 H, s), 5.43 (2H, s), 5.76 (2H, s), 6.84 (1 H, d), 6.89-
6.99 (4H, m), 7.29 (1 H, t), 7.56 (2H, d), 7.64-7.72 (2H, m), 7.77-7.85 (1 H, m), 8.23 (1 H, s), 9.66 (1 H, s).
2-((4-Amino-3-(4-hydroxyphenyl)-1 /-/-pyrazolo[3,4- c/]pyrimidin-1 -yl)methyl)-5-ethynyl-3-(4-
(methylsulfonyl)benzyl)quinazolin-4(3/-/)-one.
R' 3.46 min; m/z 578 (M+H)+ (ES+);
1H NMR (500 MHz, DMSO-d6) δ : 2.99 (3H, s), 4.42
(1 H, s), 5.41 (2H, s), 5.74 (2H, s), 6.81 -6.92 (4H, m),
7.30 (2H, d), 7.52 (2H, d), 7.69 (2H, d), 7.78-7.85 (1 H, m), 8.22 (1 H, s), 9.77 (1 H, br s).
2-((4-Amino-3-(3-hydroxyphenyl)-1 /-/-pyrazolo[3,4- c/]pyrimidin-1 -yl)methyl)-5-ethynyl-3-(4-
(trifluoromethyl)benzyl)quinazolin-4(3/-/)-one.
R' 3.67 min; m/z 568 (M+H)+ (ES+);
1H NMR (500 MHz, DMSO-d6) δ : 4.44 (1 H, s), 5.42
(2H, s), 5.75 (2H, s), 6.81 -6.86 (1 H, m), 6.86-6.90
(3H, m), 6.91 (1 H, s), 7.29 (1 H, t), 7.32 (2H, d), 7.68
(2H, dd), 7.78-7.85 (1 H, m), 8.23 (1 H, s), 9.65 (1 H, s)
3-((2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4- c/]pyrimidin-1 -yl)methyl)-5-ethynyl-4-oxoquinazolin-
3(4/-/)-yl)methyl)benzonitrile.
R' 3.30 min; m/z 525 (M+H)+ (ES+);
1H NMR (500 MHz, DMSO-d6) δ : 4.43 (1 H, s), 5.35
(2H, s), 5.72 (2H, s), 6.87 (2H, d), 6.95 (1 H, d), 7.16
(1 H, t), 7.19 (1 H, s), 7.32 (2H, d), 7.49 (1 H, d), 7.64-
7.72 (2H, m), 7.77-7.84 (1 H, m), 8.22 (1 H, s), 9.76
(1 H, br s).
Figure imgf000063_0001
Figure imgf000064_0001
(1H, brs), 9.67 (1H, s).
2-((4-Amino-3-(4-hydroxyphenyl)-1/-/-pyrazolo[3,4- c/]pyrimidin-1-yl)methyl)-3-(3-methoxybenzyl)-5-(3- methoxyprop-1-ynyl)quinazolin-4(3/-/)-one.
R' 3.50 min; m/z 574 (M+H)+ (ES+);
1H NMR (500 MHz, DMSO-d6) δ : 3.60 (3H, s), 4.34 (2H, s), 5.36 (2H, s), 5.70 (2H, s), 6.32 (1H, d), 6.39 (1H, s), 6.67 (1H, dd), 6.88 (2H, d), 6.99 (1H, t), 7.34 (2H, d), 7.54 (1H, d), 7.63 (1H, d), 7.76 (1H, t), 8.26 (1H, brs), 9.75 (1H, s).
Figure imgf000064_0002
,
4
,
Figure imgf000065_0001
s). ,
- ,
- , 3
Figure imgf000066_0001
Figure imgf000067_0001
,
- -
Figure imgf000068_0001
l
-
).
Figure imgf000069_0001
Figure imgf000070_0001
Figure imgf000071_0001
-
6- ), 4
- -
- - ), , , ,
Figure imgf000072_0001
2-Acetamido-/V-(3-(2-((4-amino-3-(3-hydroxyphenyl)- 1 /-/-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)prop-2-yn-1- yl)acetamide.
R' 3.25 min; m/z 663/665 (M+H)+ (ES+);
61 1H NMR (500 MHz, DMSO-d6) δ : 1.82 (3H, s), 3.65
(2H, d), 4.11 (2H, d), 5.29 (2H, s), 5.76 (2H, s), 6.12 (1H, d), 6.77 (1H, t), 6.81-6.86 (1H, m), 6.89-6.94 (2H, m), 7.03 (1H, t), 7.12 (1H, d), 7.26-7.32 (1H, m), 7.62 (1H, d), 7.72 (1H, d), 7.83 (1H, t), 8.11 (1H, t), 8.17
Figure imgf000073_0001
(1H, s),8.37 (1H,t), 9.69 (1H, brs).
Figure imgf000073_0002
Methyl 3-((2-((4-amino-3-(3-hydroxyphenyl)-1 H- pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-5-(6-morpholino-
6-oxohex-1 -yn-1 -yl)-4-oxoquinazolin-3(4/-/)- yl)methyl)benzoate.
R' 4.44 min; m/z 713 (M+H)+ (ES+);
65 1H NMR (500 MHz, DMSO-d6) δ : 1 .73 (2H, quin),
2.44-2.59 (4H, m), 3.25 (4H, s), 3.30-3.34 (2H, m),
3.40-3.43 (2H, m), 3.69 (3H, s), 5.39 (2H, br s), 5.73
(2H, br s), 6.79-6.86 (3H, m), 6.90 (1 H, d), 7.1 1 (1 H, t), 7.19 (1 H, s), 7.26 (1 H, t), 7.57-7.66 (3H, m), 7.75-
Figure imgf000074_0001
7.82 (1 H, m), 8.22 (1 H, br s), 9.67 (1 H, s).
2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4- d]pyrimidin-1 -yl)methyl)-3-((1 -methyl-1 /-/-pyrazol-4- yl)methyl)-5-(6-morpholino-6-oxohex-1 -yn-1 - yl)quinazolin-4(3/-/)-one.
R' 4.47 min; m/z 659 (M+H)+ (ES+);
66 1H NMR (500 MHz, DMSO-d6) δ : 1 .80 (2H, quin),
2.54-2.57 (2H, m), 2.65-2.68 (2H, m), 3.38-3.43 (4H, m), 3.47-3.55 (4H, m), 3.71 (3H, s), 5.18 (2H, s), 5.81
(2H, s), 6.86 (1 H, dd), 7.00-7.07 (2H, m), 7.24 (1 H, s),
7.29-7.36 (2H, m), 7.49-7.54 (2H, m), 7.61 -7.67 (1 H, m), 8.26 (1 H, s), 9.70 (1 H, s).
Figure imgf000074_0002
Figure imgf000074_0003
Figure imgf000075_0001
dd),
H,
Figure imgf000076_0001
6-(2-((4-Amino-3-(4-hydroxyphenyl)-1 /-/-pyrazolo[3,4- c//pyi"imidin-1 -yl)methyl)-3-(2-chlorobenzyl)-4-oxo-3,4- dihydroquinazolin-5-yl)-/V-(2-morpholinoethyl)hex-5- ynamide.
R' 1.45 min; m/z 732/734 (M+H)+ (ES+) (Method D);
76 1H NMR (400 MHz, DMSO) δ: 1 .72 (2H, m), 2.18-2.24
(4H, overlapping m), 2.29 (4H, m), 2.42 (2H, t), 3.08 (2H, q), 3.49 (4H, t), 5.28 (2H, s), 5.73 (2H, s), 6.15 (1 H, d), 6.76 (1 H, t), 6.87 (2H, d), 7.02 (1 H, t), 7.1 1 (1 H, d), 7.32 (2H, d), 7.61 (1 H, dd), 7.65-7.69 (2H, overlapping m), 7.80 (1 H, t), 8.15 (1 H, s), 9.77 (1 H, s).
Figure imgf000076_0002
Figure imgf000077_0001
Figure imgf000078_0001
6-(2-((4-Amino-3-(4-hydroxyphenyl)-1 /-/-pyrazolo[3,4- (y|pyrimidin-1 -yl)methyl)-3-(2-chlorobenzyl)-4-oxo-3,4- dihydroquinazolin-5-yl)-/V-methyl-/V-(2-(4-methyl piperazin-1 -yl)ethyl)hex-5-ynamide.
R' 1.40 min; m/z 759/761 (M+H)+ (ES+) (Method D); 1H VT NMR (400 MHz, 100°C, DMSO-d6) δ: 1 .79 (2H,
85
quin), 2.12 (3H, s), 2.25 (4H, bs), 2.33-2.36 (6H, overlapping m), 2.79 (3H, s), 3.29 (2H, t), 5.41 (2H, s), 5.68 (2H, s), 6.27 (2H, s), 6.46 (1 H, d), 6.89-6.96 (3H, overlapping m), 7.02 (1 H, m), 7.1 1 (1 H, t), 7.24 (1 H, dd), 7.37 (2H, d), 7.56 (2H, d), 7.73 (1 H, t), 8.17 (1 H,
Figure imgf000079_0001
2-((4-Amino-3-(4-hydroxyphenyl)-1/-/-pyrazolo[3,4-c/] pyrimidin-1-yl)methyl)-3-(2-chlorobenzyl)-5-(6-oxo-6- (pyrrolidin-1 -yl)hex-1 -yn-1 -yl)quinazolin-4(37)-one. R' 1.86 min; m/z 673/675 (M+H)+ (ES+) (Method D); 1H NMR (400 MHz, DMSO) δ: 1.70-1.79 (6H,
89 overlapping m), 2.43-2.50 (4H, m, partially obscured by DMSO peak), 3.23-3.4 (4H, m, partially obscured by HOD peak), 5.37 (2H, s), 5.71 (2H, s), 6.38 (1H, m), 6.55 (2H, br s), 6.90-6.92 (3H, overlapping m), 7.09 (1H, t), 7.21 (1H, d), 7.36 (2H, d), 7.59 (2H, t), 7.76 (1H, t), 8.20 (1H, s).
Figure imgf000080_0001
2-((4-Amino-3-(3-hydroxyphenyl)-1/-/-pyrazolo[3,4- c/]pyrimidin-1-yl)methyl)-3-(2-chlorobenzyl)-5-(6-(3- (dimethylamino)pyrrolidin-l -yl)-6-oxohex-1 -ynyl) quinazolin-4(3/-/)-one.
R' 1.34 min; m/z 716/718 (M+H)+ (ES+) (Method D); 1H NMR (400 MHz, DMSO-d6) δ: 1.55 (2H, br m), 1.72
92 (2H, m), 1.82 (1H, br m), 1.93 (1H, br m), 2.13 (6H, br s), 2.19 (1H, br s), 2.93 (2H, q), 3.15 (2H, m), 3.43 (1H, m), 3.55 (1H, m), 5.31 (2H, d), 5.75 (2H, s), 6.19 (1H, dd), 6.81 (1H, brt), 6.85 (1H, m), 6.92-6.95 (2H, overlapping m), 7.05 (1H, td), 7.13 (1H, dd), 7.31 (1H,
Figure imgf000080_0002
t), 7.62 (1H, m), 7.67 (1H, m), 7.80 (1H, dt), 8.18 (1H,
s), 9.66 (1H, brs).
Figure imgf000081_0001
Stereochemical Analysis of Compound Examples by Chiral, Stationary Phase HPLC
A common structural feature shared by many quinazolinone-based compounds that have been reported previously as potent PI3K inhibitors, is the presence of an aromatic nucleus bonded directly to N-3 of the bicyclic system (see for example WO 2001/081346 and WO 2008/127226). Depending upon the exact nature of the aryl substituent, this motif can give rise to sterically compressed bi-aryls, in which the rotational degree of freedom around the N-3 to C-aryl single bond is dramatically reduced. Compelling evidence has been obtained that in the case of compounds incorporating an o f/?o-substituted benzene ring, bond rotation can be so hindered as to give rise to discrete confomers. For example, chiral stationary phase HPLC analysis of PIK294 (Example 'S3' from WO 2008/127226) indicates that the compound exists as readily separable, non-superimposable stereoisomers, that is as a 1 :1 mixture of enantiomeric atropisomers (Fig. 1 ). This assigment was confirmed by the separation of a sample of PIK294, using chiral stationary phase preparative HPLC, into its discrete atropisomers (Fig. 2). As expected these enantiomeric compounds exhibited identical proton NMR spectra (data not shown) and, at both ambient and elevated temperatures, showed no detectable signs of interconversion over several days, indicating that the discrete rotational isomers have indefinitely long half lives under physiological conditions. The presence of stable atropisomers was also demonstrated for two additional N-3 aryl quinazolinones: the mercaptopurine derivative 20 (Example D-026 from WO 2001/081346) and a second pyrazolopyrimidine, analogue 21 , revealing that this is a generic structural feature of this chemotype (Fig. 3). In contrast, over twenty compounds, selected from the exemplified structures disclosed above (Examples 1 , 9, 10, 11 , 12, 13, 14, 15, 16, 17, 18, 19, 20, 21 , 22, 23, 24, 25, 31 , 32, 33, 34, and 38) were screened using chiral stationary phase HPLC (16 different conditions) and no evidence for atropisomerism was observed. The stereochemical implications of atropisomerism have been described recently as 'a lurking menace with the potential to significantly increase the cost of pharmaceutical research and development if ignored.' (Claydon, J. et al., Angew. Chem. Int. Ed., 2009, 48, 6398-6401 ).
The additional complexity and consequences for drug development resulting from atropisomerism are analogous to those arising from other sources of molecular isomerism such as the presence of a stereogenic centre. This property renders molecules such as 20 and 21 (Fig. 3) both chiral, and unless resolved, a racemic mixture; the components of which could possess different pharmacological and toxicological profiles. This feature is likely to significantly increase downstream development costs for such molecules, and the absence of atropisomerism in the compounds disclosed herein is therefore a highly desirable and advantageous property. Enzyme inhibition assay
PI3 kinases catalyse the phosphorylation of phosphatidylinositol 4,5-biphosphate (PIP2) to phosphatidylinositol 3,4,5-triphosphate (PIP3) in the presence of ATP and Mg2+ ions. The PIP3 product can be detected by displacement of biotin-PIP3 from energy transfer complexes consisting of europium labelled anti-GST monoclonal antibody, a GST-tagged Pleckstrin homology (PH) domain, biotinylated PIP3 and streptavidin-allophycocyanin (APC) by the time- resolved fluorescence resonance energy transfer (TR-FRET) (HTRFOPI3K enzyme assay, Millipore). Excitation (330 nm) of europium in the complex results in an energy transfer to the APC and a fluorescent emission at 665 nm although europium itself emits at its characteristic 620 nm. The PIP3 product formed by PI3K activity displaces biotin-PIP3 from the complex and results in a loss of energy transfer (decreasing signal).
The compound to be tested was added, at the desired final concentrations, to a mixture of PIP2 substrate and recombinant PI3 kinase α, δ or γ enzymes (Millipore), and the mixture incubated for 2 hr at RT. Following this incubation period, ATP (20 μΜ) was added to the enzyme/compound/PIP2 substrate mixture and the resulting mixture was incubated for 30 min at RT. A stopping solution containing biotinylated PIP3 and the detection mix containing the GST tagged GRP1 pleckstrin homology (PH) domain and fluorophores were then added and the mixture was incubated at RT for 15-18 hr, prior to detection in a fluorescence microplate reader (Varioskan® Flash, ThermoFisher Scientific).
The results were calculated according to the formula: APC signal (emission at 665 nm)/europium signal:(emission at 620 nm) x 104. The percentage inhibition of each reaction was calculated relative to DMSO treated control, and the 50% inhibitory concentration (IC50 value) then calculated from the concentration-response curve (Table 3 and Table 4).
PI3K5 cell based assay As a means of assessing PI3K δ activation in response to stimuli, the phosphorylation status of the protein, Akt, a downstream product of PI3K5, signaling was determined.
Human monocytic cells (U937 cells), were differentiated to macrophage-type cells by incubation with phorbol myristate acetate (PMA; 100 ng/mL) for 48 to 72 hr. Cells were then pre-incubated with either the test compound or vehicle for 2 hr and were then stimulated briefly by exposure to H202 (10 mM; 5-7 min) and the reaction stopped by replacing the media with 4% formaldehyde solution. Endogenous peroxide activity and formaldehyde were inactivated by incubating with quenching buffer (0.1 % sodium azide, 1 % H202 in PBS with 0.1 % Triton X-100) for 20 min. The cells were washed with buffer (PBS containing 0.1 % Triton X-100) and were incubated with blocking solution (1 % BSA in PBS) for 1 hr and were then re-washed with buffer and incubated overnight with either anti-pAkt antibody or anti-pan-Akt antibody (both from Cell Signaling Technology). After washing with buffer (PBS containing 0.1 % Triton X-100), cells were incubated with an HRP-conjugated secondary antibody (Dako) and the resultant signal was determined colorimetrically (OD: 450 nm with a reference wavelength of 655 nm) using TMB substrate (substrate reagent pack supplied by R&D Systems, Inc.).
This reaction was stopped by addition of 100 μΙ_ of 1 N H2S04 solution. Cells were then washed with buffer (PBS containing 0.1 % Triton X-100) and 100 μΙ_ of 5% crystal violet solution was applied for 30 min. After washing with buffer (PBS containing 0.1 % Triton X-100) 100 μΙ_ of 1 % SDS was added to each well and the plates were shaken lightly for 1 hr prior to measuring the absorbance at 595 nm (Varioskan® Flash, Thermo-Fisher Scientific). The measured OD450-655 readings were corrected for cell number by dividing the OD450-655 by the OD595 readings. The ratio of pAkt signal to total Akt signal was used to quantitate the extent of PI3K δ activation. The percentage inhibition for each well was calculated relative to a 10 μg mL standard control (LY294002) set to 100% inhibition versus H202-only controls as 0% inhibition. The IC50 values were calculated from the concentration-response curves generated by the serial dilutions of the test compounds (Table 3 and Table 4).
MTT assay
PMA-differentiated U937 cells were pre-incubated with compound for 4 hr in 5% FCS or 10% FCS for 24 hr. The supernatant was replaced with 200 μΙ_ of new media and 10 μΙ_ of MTT stock solution (5 mg/ml) added to each well. After 1 hr incubation, the media were removed, 200 μΙ_ of DMSO added to each well and the plates were shaken lightly for 1 hr prior to reading the absorbance at 550 nm. The percentage loss of cell viability was calculated for each well relative to vehicle (0.5% DMSO)-treatment (Table 3). Table 3: In Vitro Screening Data: Kinase Isozyme and Cellular Activity Ranges for Exemplified Compounds
Cellular Activity Cell Viability
PI3 Kinase Inhibition
IC5o values (H202 MTT Assay in D-U937 values at stated Isozyme '
induced pAkt 1 cells c
Example δ Y a D-U937 Cells at 4 hr at 24 hr
1 ++ + + ++ - -
2 ++ + - ++ - -
3 ++ ++ ++ ++ - -
4 ++ ++ ++ ++ - -
5 ++ ++ ++ ++ - -
6 ++ + + ++ - - ++ + + ++ - -
++ + + ++ - -
++ + + ++ - -
++ + + ++ - -
++ + + ++ - -
++ + + ++ + -
++ + + ++ - -
++ + + ++ - -
++ + - ++ - -
++ + + ++ - -
++ - + ++ - -
++ + + ++ - -
++ + + ++ - -
++ + + ++ - -
++ + + ++ - -
++ + + ++ - -
++ + + ++ - -
++ + + ++ - -
++ + + ++ - -
++ + + ++ - -
++ + + ++ - -
++ + + ++ - -
++ + + ++ - -
++ + + ++ - - ++ + + ++ - -
++ + + ++ - -
++ + + ++ - -
++ + + ++ - -
++ + + ++ - -
++ - + ++ - -
++ - + ++ - -
++ + + ++ - -
++ + ++ ++ - -
++ + + ++ - -
++ + + ++ - -
++ + + ++ - -
++ + + ++ - -
++ + - ++ - -
++ + + ++ - -
++ + + ++ - -
++ + + ++ - -
++ + + ++ - -
++ + + ++ - -
++ + + ++ - -
++ + + ++ - -
++ ++ + ++ - -
++ ++ ++ ++ - -
++ ++ ++ ++ - - ++ ++ + ++ - -
++ ++ ++ ++ - -
++ ++ + ++ - -
++ ++ + ++ - -
++ ++ + ++ - -
++ ++ + ++ - -
++ ++ + ++ - -
++ ++ + ++ - -
++ ++ + ++ - -
++ ++ + ++ - -
++ ++ + ++ - -
++ ++ + ++ - +
++ ++ + ++ - -
++ ++ + ++ - -
++ ++ + ++ + -
++ ++ + ++ - -
++ ++ + ++ - -
++ ++ + ++ - -
++ ++ + ++ - -
++ ND ND ND - -
++ ++ + ++ - -
++ ++ + ++ - -
++ ++ + ++ - -
++ ++ + ++ - - 79 ++ + + ++ - -
80 ++ ND ND + - -
81 ++ + + ++ - -
82 ++ ++ + ++ - -
83 ++ ND ND ++ - -
84 ++ ND ND ++ - -
85 ++ ND ND ++ - -
86 ++ ++ ++ ++ - -
87 ++ ND ND ++ - -
88 ++ ++ + ++ - -
89 ++ ++ - ++ - -
90 ++ ++ + ++ - -
91 ++ ++ + ++ - -
92 ++ ND ND + - -
93 ++ ++ + ++ - -
94 + ND ND + - -
95 ++ ++ + ++ - - a) ++ IC50 <50 nM, + IC50 <1000 nM, - >1000 nM. b) ++ IC50 <10 nM; + IC50 <1000 nM; c) - <30%; + >30%. ND: not done
Table 4: In Vitro Screening Data: Kinase Isozyme and Cellular Activity Values for Selected Exemplified Compounds
Test PI3 Kinase Inhibition Cellular Activity
Cmpd IC5o values at stated Isozyme (nM) IC50 values (H202 induced pAkt (nM)
Example δ Y a D-U937 Cells
1 7 234 199 1 .1
4 12 20 17 1 .8 5 20 16 20 0.58
15 4 289 1966 0.57
37 12 1613 474 0.33
86 36 22 27 0.41
89 3 47 1486 0.2
LPS-induced neutrophilia in the mouse: determination of effect and duration of action Non-fasted mice were dosed by the intra-tracheal route with either vehicle, or the test substance at the time points ("pre-dose") indicated with respect to the start of LPS treatment. At T = 0, mice were placed into an exposure chamber and exposed to LPS. Eight hr after LPS challenge, animals were anesthetized, the trachea cannulated and BALF extracted by infusing and withdrawing 1 mL of PBS into the lungs via a tracheal catheter. Total and differential white cell counts in the BALF samples were measured using a Neubaur haemocytometer. Cytospin smears of the BALF samples were prepared by centrifugation at 200 rpm for 5 min at RT and stained using a DiffQuik stain system (Dade Behring). Cells were counted using oil immersion microscopy. The results for the compound of Example 1 are shown in Fig. 4. The upper panel reveals the effect on BAL neutrophil numbers following the administration of Example 1 when the compound was dosed 2 hr before LPS challenge. The lower panel shows the results of a time course experiment on the inhibition of BAL neutrophilia by Example 1 when the compound is administered at different time points prior to endotoxin challenge. Data for neutrophil numbers is reported as total and differential number (test substance relative to vehicle) of cells per mL of BALF, mean ± S.E.M. (n = 8).
The profiles of two additional compounds: the carboxylic acid derivative, Example 4, and the mopholine amide, Example 5, were determined in this model of pulmonary inflammation. Treatment of the animals with the test compounds 2 hr before challenge with endotoxin, produced a comparable and dose dependent inhibition of the evoked inflammatory response as determined by the measurement of neutrophil influx into the lung (Table 5).
Table 5: The effect of escalating doses of compound Example 4 and Example 5 on the inhibition of LPS induced neutophilia in mouse lung
Test Compound % Inhibition of neutrophils in BAL at the dose indicated
(mg/mL)
Example No. (0 05) (0.2) (1 .0)
4 3.2 47.2 64.6 4.8 37.7 58.5
However, by increasing the period between drug treatment and the subsequent LPS challenge from 2 to 8 hr it was possible to distinguish a difference in the duration of action of the two compounds when administered at the same dose. Under these test conditions the level of antiinflammatory activity of compound Example 5 was still over half of its original value, as determined 2hr after drug treatment, at the 8 hr time point. (Table 6). In contrast, under the same conditions, the remaining anti-inflammatory effect of compound Example 4 was reduced by 90%, to an insignificant level (<5% inhibition), demonstrating that its pharmacological activity is much less sustained.
Table 6: Comparison the activities of compounds Example 4 and Example 5 to inhibit an evoked (LPS) inflammatory response in mouse lung 2 and 8 hr after administration
Test Compound % Inhibition at 0.2 mg/mL dose % Reduction of
at time indicated (hr) inhibition at 8hr
compared with 2hr
Example No. (2) (8)
4 47.2 12.9 73
5 37.7 21.2 44
Cigarette Smoke Model
A/J mice (males, 5 weeks old) were exposed to cigarette smoke (4% cigarette smoke, diluted with compressed air) for 30 min/day for 1 1 days using a Tobacco Smoke Inhalation Experiment System for small animals (Model SIS-CS; Sibata Scientific Technology, Tokyo, Japan). Test substances were given intra-nasally (35 μί of solution in 50% DMSO/PBS) and therapeutically twice daily for 3 days after the final cigarette smoke exposure. Twelve hr after the last dosing, animals were anesthetized, the trachea cannulated and bronchoalveolar lavage fluid (BALF) was collected. The numbers of alveolar macrophages and neutrophils were determined by FACS analysis (EPICS® ALTRA II, Beckman Coulter, Inc., Fullerton, CA, USA) using anti- mouse MOMA2 antibody (macrophage) or anti-mouse 7/4 antibody (neutrophil).
The results for the compound of Example 1 are shown in Fig. 5 for neutrophils (upper panel) and for activated alveolar macrophages (lower panel). The cigarette smoke model used for this study is reported to be a corticosteroid refractory system, [To, Y. et al., Am. J. Respir. Crit. Care Med., 2010, 182:897-904; Medicherla, S. et al., J. Pharmacol. Exp. Then 2008, 324:921 -9] and the data reveal that dexamethasone (0.3-10 mg/kg, p.o.) was inactive. The results obtained for Example 1 demonstrate that the compound possesses anti-inflammatory activity when administered as a monotherapy. Moreover, when Example 1 was co-administered with fluticasone propionate, at a dose which lacks effect as monotherapy, a marked enhancement of anti-inflammatory activtiy was detected. Data for cell numbers are shown as the mean ± SEM.
Throughout the specification and the claims which follow, unless the context requires otherwise, the word 'comprise', and variations such as 'comprises' and 'comprising', will be understood to imply the inclusion of a stated integer, step, group of integers or group of steps but not to the exclusion of any other integer, step, group of integers or group of steps.
All patents and patent applications referred to herein are incorporated by reference in their entirety.
The application of which this description and claims forms part may be used as a basis for priority in respect of any subsequent application. The claims of such subsequent application may be directed to any feature or combination of features described herein. They may take the form of product, composition, process, or use claims and may include, by way of example and without limitation, the claims.

Claims

A compound of formula (I)
Figure imgf000092_0001
wherein
R1 is H , a saturated or unsaturated, branched or unbranched CMS alkyl chain, wherein optionally one or more carbons (for example 1 , 2 or 3 carbons, suitably 1 or 2, in particular 1 ) is/are replaced by a heteroatom selected from O, N, S(0)p, (for example a CH2, group is replaced with O, or with NH, or with S, or with S02 or a -CH3 group at the terminus of the chain or on a branch is replaced with OH or with NH2) wherein said chain is optionally substituted by one or more groups (for example 1 to 3, such as 2 groups) independently selected from oxo, halogen, an aryl group, a heteroaryl group, a carbocyclyl group or a heterocyclyl group,
each aryl, heteroaryl, carbocyclyl or heterocyclyl group bearing:
0 to 3 substituents selected from halogen, -hydroxyl, -Ci-6 alkyl, -Ci-6 alkoxy, -C2-3 alkoxyOCi-3 alkyl, -C2-3 alkylOCi-3 alkyl, -Ci-3 hydroxyalkyl, -Ci-6 haloalkyl, amino, -Ci-4 mono or -C2-8 di-alkyl amino, -Ci-4 mono or -C2-8 di-acyl amino, -C0-6 alkylS(0)pCi-6 alkyl, -C0-6 alkylS(0)pNR6R7, -Co-6 alkylNR8C0-6alkylS(O)pCi-6 alkyl, -C0-6 alkylC(O)OC0-6 alkyl, -NR8C0-6 alkylC(0)NR6R7 -NR8C0-6 alkylC(O)C0-6 alkyl, -C0-6 alkylC(0)NR6R7, and -C0-6 alkylC(0)Ci-6 alkyl; and/or one aryl, heterocyclyl or carbocyclyl; X is C6-io aryl or a C5-9 heteroaryl each substituted by R2a and by R2b
wherein R2a is selected from hydrogen, -Ci-3 alkyl, halo, hydroxyl, cyano, -Ci-3 haloalkyl, -Ci-3 alkoxy, -C2-3 alkoxyOCi-3 alkyl, -C2-3 alkylOCi-3 alkyl, -Ci-3 hydroxyalkyl, -C0-6 alkylS(0)qCi-3 alkyl, -C0-6 alkylS(0)pNR6R7, -C0-6 alkylNR8C0- 6alkylS(0)pCi-6 alkyl, -C0-6 alkylC(0)OH, -C0-6 alkylC(0)OCi-6 alkyl, -NR8C0-6 alkylC(0)NR6R7, -NR8C0-6 alkylC(0)Ci-6 alkyl, -C0-6 alkylC(0)NR6R7 and -C0-6 alkylC(0)Ci-6 alkyl; and
R2b is selected from hydrogen, Ci-3 alkyl, halo, hydroxyl, cyano, -Ci-3 haloalkyl, -Ci-3 alkoxy, and -C0-6 alkylS(0)qCi-3 alkyl;
R3a is hydroxyl;
R3b is selected from hydrogen, hydroxyl, halo, cyano, -Ci-3 haloalkyl, -Ci-3 hydroxyalkyl, -Ci-3 alkoxy, and -S(0)qCi-3 alkyl; R4 is hydrogen or -Ci-3 alkyl;
R5 is hydrogen or -Ci-3 alkyl;
R6 is hydrogen or -Ci-6 alkyl;
R7 is hydrogen or -Ci-6 alkyl;
R8 is hydrogen or -Ci-6 alkyl;
p is 0 or an integer 1 or 2;
q is 0 or an integer 1 or 2
or a pharmaceutically acceptable salt thereof, including all stereoisomers, tautomers and isotopic derivatives thereof.
2. A compound of formula (I) according to claim 1 wherein:
R1 is H , a saturated or unsaturated, branched or unbranched CMO alkyl chain, wherein optionally one or more carbons (for example 1 , 2 or 3 carbons, suitably 1 or 2, in particular 1 ) is/are replaced by a heteroatom selected from O, N, S(0)p, (for example a CH2, group is replaced with O, or with NH, or with S, or with S02 or a -CH3 group at the terminus of the chain or on a branch is replaced with OH or with NH2) wherein said chain is optionally substituted by one or more groups (for example 1 to 3, such as 2 groups) independently selected from oxo, halogen, an aryl group, a heteroaryl group, a carbocyclyl group or a heterocyclyl group,
each aryl, heteroaryl, carbocyclyl or heterocyclyl group bearing:
0 to 3 substituents selected from halogen, -Ci-6 alkyl, -Ci-6 alkoxy, -C2-3 alkoxyOCi-3 alkyl, -Ci-6 haloalkyl, amino, -Ci-4 mono or -C2-8 di-alkyl amino, -Ci-4 mono or -C2-8 di-acyl amino, -C0-6 alkylS(0)pCi-6 alkyl, -C0-6 alkylS(0)pNR6R7, -C0-6 alkylNR8Co-6alkylS(0)pCi-6 alkyl, -C0-6 alkylC(O)OC0-6 alkyl, -NC0-6 alkylC(0)NR6R7 -NC0-6 alkylC(O)C0-6 alkyl, -C0-6 alkylC(0)NR6R7, and -C0-6 alkylC(0)d-6 alkyl; and/or one aryl, heterocyclyl or carbocyclyl;
X is C6-io aryl or a C5-9 heteroaryl each substituted by R2a and optionally by R2b wherein R2a is selected from hydrogen, -Ci-3 alkyl, halo, hydroxyl, cyano, -C1-3 haloalkyl, -Ci-3 alkoxy, -C2-3 alkoxyOCi-3 alkyl, -Ci-3 hydroxyalkyl, -Co-6 alkylS(0)qCi-3 alkyl, -C0-6 alkylS(0)pNR6R7, -C0-6 alkylNR8C0-6alkylS(O)pCi-6 alkyl, -C0-6 alkylC(0)OH, -C0-6 alkylC(0)OCi-6 alkyl, -NC0-6 alkylC(0)NR6R7, -NR8Co-6 alkylC(0)Ci-6 alkyl, -C0-6 alkylC(0)NR6R7 and -C0-6 alkylC(0)Ci-6 alkyl; and
R2b is selected from hydrogen, Ci-3 alkyl, halo, cyano, -Ci-3 haloalkyl, -Ci-3 alkoxy and -C0-6 alkylS(0)qCi-3 alkyl;
R3a is hydroxyl;
R3b is hydrogen, hydroxyl, halo, cyano, -Ci-3 haloalkyl, -Ci-3 hydroxyalkyl, -Ci-3 alkoxy, -S(0)qCi-3 alkyl;
R4 is hydrogen or -Ci-3 alkyl;
R5 is hydrogen or -Ci-3 alkyl;
R6 is hydrogen or -Ci-6 alkyl;
R7 is hydrogen or -Ci-6 alkyl;
R8 is hydrogen or -Ci6 alkyl p is 0 or an integer 1 or 2;
q is 0 or an integer 1 or 2
or a pharmaceutically acceptable salt thereof, including all stereoisomers, tautomers and isotopic derivatives thereof.
3. A compound of formula (I) according to claim 1 or 2, wherein R1 is hydrogen.
4. A compound of formula (I) according to claim 1 or 2 wherein R1 is:
Figure imgf000094_0001
5. A compound of formula (I) according to claim 1 or 2 wherein R1 is -CH2CH2CH2C(0)OH.
6. A compound of formula (I) according to claim 1 or 2 wherein R1 is -CH2OCH2CH2OCH2CH2OCH3.
7. A compound of formula (I) according to any one of claims 1 to 6, wherein R2a is selected from the group comprising chloro, fluoro, cyano, methoxy, trifluoromethyl and S02CH3.
8. A compound of formula (I) according to any one of claims 1 to 7, wherein R4 is hydrogen.
9 A compound of formula (I) according to any one of claims 1 to 8, wherein R5 is hydrogen.
10 A compound of formula (I) according to any one of claims 1 to 9, wherein R3 is in the meta position.
1 1 . A compound of formula (I) according to any one of claims 1 to 9, wherein R3 is in the para position.
12. A compound of formula (I) according to any one of claims 1 to 1 1 , wherein carbocyclyl is a C3-10 saturated or partially saturated carbocyclic ring systems, for example cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl.
13. A compound of formula (I) according to any one of claims 1 to 12, wherein heteroaryl is a C5-9 membered aromatic carbocylic ring or bicyclic ring system comprising one or more, (for example 1 , 2, 3 or 4) heteroatoms independently selected from O, N and S.
14. A compound of formula (I) according to any one of claims 1 to 13, wherein heterocyclic is intended to refer to a 5 to 10 membered ring system which is saturated or partially unsaturated and which is non-aromatic comprising one or more (for example 1 , 2 or 3 in particular 1 or 2,) heteroatoms independently selected from O, N and S,
15. A compound of formula (I) according to any one of claims 1 to 13, wherein aryl is C6-14 mono or polycyclic groups (such as C6-io mono or bicyclic groups) having from 1 to 3 rings wherein at least one ring is aromatic.
16. A compound of formula (I) according to claim 1 , wherein the compound is selected from:
2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-d]pyrimidin-1 -yl)methyl)-3-(2- chlorobenzyl)-5-ethynylquinazolin-4(3H)-one;
2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-d]pyrimidin-1 -yl)methyl)-3-(2- chlorobenzyl)-5-(3-(2-(2-methoxyethoxy)ethoxy)prop-1 -yn-1 -yl)quinazolin-4(3H)-one; 2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-d]pyrimidin-1 -yl)methyl)-3-(2- chlorobenzyl)-5-(6-morpholino-6-oxohex-1 -yn-1 -yl)quinazolin-4(3H)-one; 6-(2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-d]pyrimidin-1 -yl) methyl)-3-(2-chlorobenzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)hex-5-ynoic acid;
2- ((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-d]pyrimidin-1 -yl)methyl ■3-(2- chlorobenzyl)-5-(6-morpholino-6-oxohex-1 -yn-1 -yl)quinazolin-4(3H)-one;
3- ((2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-d]pyrimidin-1 -yl)me hyl)-5-(3-(2-(2- hydroxyethoxy)ethoxy)prop-1 -yn-1 -yl)-4-oxoquinazolin-3(4H)-yl)methyl)benzonitrile;
2-((4-Amino-3-(3-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin- -yl)methyl ■3-(2-chloro benzyl)-5-(3-(2-morpholinoethoxy)prop-1 -ynyl)quinazolin-4(3/-/)-
2-((4-Amino-3-(4-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin -yl)methyl ■3-(2- chlorobenzyl)-5-ethynylquinazolin-4(3/-/)-one;
2-((4-Amino-3-(3-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin -yl)methyl ■3-(3-chloro benzyl)-5-ethynylquinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin -yl)methyl ■3-(3-chloro benzyl)-5-ethynylquinazolin-4(3/-/)-one;
2-((4-Amino-3-(3-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin -yl)methyl ■5-ethynyl-3- (2-fluorobenzyl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin -yl)methyl ■5-ethynyl-3- (2-fluorobenzyl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin -yl)methyl ■5-ethynyl-3- (3-methoxybenzyl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(3-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin -yl)methyl ■5-ethynyl-3- (3-methoxybenzyl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin -yl)methyl ■5-ethynyl-3- (3-(trifluoromethyl)benzyl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(3-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin -yl)methyl ■5-ethynyl-3- (3-(trifluoromethyl)benzyl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin -yl)methyl ■3-(4- chlorobenzyl)-5-ethynyl quinazolin-4(3/-/)-one.;
2-((4-Amino-3-(3-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin -yl)methyl ■5-ethynyl-3- (4-(methylsulfonyl)benzyl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin -yl)methyl ■5-ethynyl-3- (4-(methylsulfonyl)benzyl)quinazolin-4(3/-/)-one;
2- ((4-Amino-3-(3-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin -yl)methyl ■5-ethynyl-3- (4-(trifluoromethyl)benzyl)quinazolin-4(3/-/)-one;
3- ((2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)me hyl)-5-ethynyl-
4- oxoquinazolin-3(4/-/)-yl)methyl)benzonitrile;
2- ((4-Amino-3-(3-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin -yl)methyl ■5-ethynyl-3- (3-(methylsulfonyl)benzyl)quinazolin-4(3/-/)-one;
3- ((2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)me hyl)-5-ethynyl-
4- oxoquinazolin-3(4/-/)-yl)methyl)benzonitrile;
2-((4-Amino-3-(3-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin -yl)methyl ■3-(4-chloro benzyl)-5-ethynylquinazolin-4(3/-/)-one;
2-((4-Amino-3-(3-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin -yl)methyl ■3-(4-chloro benzyl)-5-(3-methoxyprop-1 -ynyl)quinazolin-4(3/-/)-one; 2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,^^
benzyl)-5-(3-methoxyprop-1 -ynyl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-5-(3-methoxyprop-1 -ynyl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-(^pyrimidin-1 -yl)methyl)-5-ethynyl-3- (4-(trifluoromethyl)benzyl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-5-(3-(2-methoxyethoxy)prop-1 -ynyl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-(^pyrimidin-1 -yl)methyl)-5-ethynyl-3- ((5-methylisoxazol-3-yl)methyl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-(^pyrimidin-1 -yl)methyl)-5-ethynyl-3- ((5-methylisoxazol-3-yl)methyl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(3-chloro-2- fluorobenzyl)-5-ethynylquinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2,6- difluorobenzyl)-5-ethynylquinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(4-chloro-2- fluorobenzyl)-5-ethynylquinazolin-4(3/-/)-one;
2-((4-Amino-3-(3-fluoro-4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2- chlorobenzyl)-5-ethynylquinazolin-4(3/-/)-one;
2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-(^pyrimidin-1 -yl)methyl)-5-(3-methoxy prop-1 -ynyl)-3-(3-(trifluoromethyl)benzyl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-(^pyrimidin-1 -yl)methyl)-5-ethynyl-3- (4-fluorobenzyl) quinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-5-(3-cyclopentylprop-1 -ynyl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-5-(3-
(benzyloxy)prop-1 -ynyl)-3-(2-chlorobenzyl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-5-(5-hydroxypent-1 -ynyl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-(^pyrimidin-1 -yl)methyl)-5-ethynyl-3- (2-fluoro-5-methoxybenzyl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(3,4- dichlorobenzyl)-5-ethynylquinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-(^pyrimidin-1 -yl)methyl)-3-benzyl-5- ethynylquinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-(^pyrimidin-1 -yl)methyl)-5-ethynyl-3- (2-trifluoromethylbenzyl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-(^pyrimidin-1 -yl)methyl)-5-ethynyl-3- (4-methoxybenzyl)quinazolin-4(3/-/)-one;
4-((2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-5-ethynyl- 4-oxoquinazolin-3(4/-/)-yl)methyl)benzonitrile;
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-(^pyrimidin-1 -yl)methyl)-5-ethynyl-3- (2-fluoro-4-methoxybenzyl)quinazolin-4(3/-/)-one; 1 -(3-(2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c ]pyrimidin-1 -yl)methyl)-3-(2- chlorobenzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)prop-2-ynyl)urea;
2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2- fluorobenzyl)-5-(3-(2-(2-methoxyethoxy)ethoxy)prop-1 -ynyl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-fluoro-3-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2- chlorobenzyl)-5-ethynylquinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-5-(3-phenoxyprop-1 -ynyl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-fluoro benzyl)-5-(6-morpholino-6-oxohex-1 -yn-1 -yl)quinazolin-4(3/-/)-one;
6-(2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidiri-1 -yl)rTiethyl)-3-(2- chlorobenzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)-/V-(2-methoxyethyl)hex-5-ynamide;
2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-5-(7-morpholino-7-oxohept-1 -yn-1 -yl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-5-(5-morpholino-5-oxopent-1 -yn-1 -yl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-d7pyi"iniidin-1 -yl)methyl)-3-((5-methyl pyrazin-2-yl)methyl)-5-(6-morpholino-6-oxohex-1 -yn-1 -yl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-5-(6-oxo-6-(piperidin-1 -yl)hex-1 -yn-1 -yl)quinazolin-4(3/-/)-one;
6- (2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)-/V,/V-diethylhex-5-ynamide;
7- (2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)hept-6-ynoic acid;
2-Acetamido-N-(3-(2-((4-amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl) methyl)-3-(2-chlorobenzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)prop-2-yn-1 -yl)acetamide;
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-(^pyrimidin-1 -yl)methyl)-3-(3-methoxy
-5-(trifluoromethyl)benzyl)-5-(6-morpholino-6-oxohex-1 -yn-1 -yl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-(^pyrimidin-1 -yl)methyl)-3-(2-methoxy phenethyl)-5-(6-morpholino-6-oxohex-1 -yn-1 -yl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-
(benzo[b]thiophen-2-ylmethyl)-5-(6-morpholino-6-oxohex-1 -yn-1 -yl)quinazolin-4(3/-/)- one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-fluoro-3- methoxybenzyl)-5-(6-morpholino-6-oxohex-1 -yn-1-yl)quinazolin-4(3/-/)-one;
Methyl 3-((2-((4-amino-3-(3-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-5-
(6-morpholino-6-oxohex-1 -yn-1 -yl)-4-oxoquinazolin-3(4/-/)-yl)methyl)benzoate;
2-((4-Amino-3-(3-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-((1 -methyl-
1 H-pyrazol-4-yl)methyl)-5-(6-morpholino-6-oxohex-1 -yn-1 -yl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-
(benzofuran-5-ylmethyl)-5-(6-morpholino-6-oxohex-1 -yn-1 -yl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]py
thiazol-4-yl)methyl)-5-(6-morpholino-6-oxohex-1 -yn-1 -yl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-5-(6-(4-methylpiperazin-1 -yl)-6-oxohex-1 -yn-1 -yl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chloro benzyl)-5-(6-(4-morpholinopiperidin-1 -yl)-6-oxohex-1 -yn-1 -yl)quinazolin-4(3/-/)-one;
5-(6-(4-Acetylpiperazin-1 -yl)-6-oxohex-1 -yn-1 -yl)-2-((4-amino-3-(4-hydroxyphenyl)-1 H- pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chlorobenzyl)quinazolin-4(3/-/)-one;
/V-(4-(2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-c ]pynmidin-1 -yl)rTiethyl)-3-(2- chlorobenzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)but-3-yn-1 -yl)morpholine-4-carboxamide;
5- (6-(4-Acetylpiperazin-1 -yl)-6-oxohex-1 -yn-1 -yl)-2-((4-amino-3-(4-hydroxyphenyl)-1 H- pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2-chlorobenzyl)quinazolin-4(3/-/)-one;
/V-(4-(2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-c ]pynmidin-1 -yl)rTiethyl)-3-(2- chlorobenzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)but-3-yn-1 -yl)morpholine-4-carboxamide; 2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-5-(5-(bis(2- methoxyethyl)amino)pent-1 -ynyl)-3-(2-chlorobenzyl)quinazolin-4(3/-/)-one;
6- (2-((4-Amino-3-(4-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2- chlorobenzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)-/V-cyclopentylhex-5-ynamide;
6-(2-((4-Amino-3-(4-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2- chlorobenzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)-/V-(tetrahydro-2/-/-pyran-4-yl)
hex-5-ynamide;
6-(2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/7pynrnidin-1 -yl)methyl)-3-(2- chlorobenzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)-/V-(2-morpholinoethyl)hex-5-ynamide;
2-((4-Amino-3-(4-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2- chlorobenzyl)-5-(6-(4-(2-methoxyethyl)piperazin-1-yl)-6-oxohex-1 -ynyl)
quinazolin-4(3/-/)-one;
6-(2-((4-Amino-3-(4-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2- chlorobenzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)-/V-(2-(dimethylamino)ethyl)
hex-5-ynamide;
6-(2-((4-Amino-3-(4-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2- chlorobenzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)-/V-(pyridin-4-yl)hex-5-ynamide;
6-(2-((4-Amino-3-(3-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2- chlorobenzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)-/V-(pyridin-4-yl)hex-5-ynamide;
2-((4-Amino-3-(4-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2- chlorobenzyl)-5-(6-(4-(dimethylamino)piperidin-1 -yl)-6-oxohex-1 -ynyl)
quinazolin-4(3/-/)-one;
6-(2-((4-Amino-3-(4-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2- chlorobenzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)-/V,/V-bis(2-methoxyethyl)hex-5-ynamide;
6-(2-((4-Amino-3-(3-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2- chlorobenzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)-/V,/V-bis(2-methoxyethyl)hex-5-ynamide;
6-(2-((4-Amino-3-(4-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2- chlorobenzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)-/V-(2-(4-methylpiperazin-1 -yl)ethyl)hex-
5- ynamide;
6- (2-((4-Amino-3-(4-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2- chlorobenzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)-/V-methyl-/V-(2-(4-methyl
piperazin-1 -yl)ethyl)hex-5-ynamide;
6-(2-((4-Amino-3-(4-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2- chlorobenzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)-/V-isopropylhex-5-ynamide;
6-(2-((4-Amino-3-(3-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3 chlorobenzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)-/V-isopropylhex-5-ynamide;
6-(2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(^ chlorobenzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)-/V,/V-dimethylhex-5-ynami
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2- chlorobenzyl)-5-(6-oxo-6-(pyrrolidin-1 -yl)hex-1 -yn-1 -yl)quinazolin-4(3/-/)-one;
6-(2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(^ chlorobenzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)-/V-(pyrrolidin-3-yl)hex-5-ynamid
2-((4-Amino-3-(4-hydroxyphenyl)-1 H-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2- chlorobenzyl)-5-(6-(3-(dimethylamino)pyrrolidin-1 -yl)-6-oxohex-1 -ynyl)quinazolin one;
2-((4-Amino-3-(3-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2- chlorobenzyl)-5-(6-(3-(dimethylamino)pyrrolidin-1 -yl)-6-oxohex-1 -ynyl)quinazolin-4(3/-/)- one;
2-((4-Amino-3-(4-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2- chlorobenzyl)-5-(6-(4-methyl-1 ,4-diazepan-1 -yl)-6-oxohex-1 -ynyl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(3-hydroxyphenyl)-1 /-/-pyrazolo[3,4-c/]pyrimidin-1 -yl)methyl)-3-(2- chlorobenzyl)-5-(6-(4-methyl-1 ,4-diazepan-1 -yl)-6-oxohex-1 -ynyl)quinazolin-4(3/-/)-one;
2-((4-Amino-3-(4-hydroxy-3-methoxyphenyl)-1 H-pyrazolo[3,4-(^pyrimidin-1 -yl)methyl)-3-
(2-chlorobenzyl)-5-(6-morpholino-6-oxohex-1 -ynyl)quinazolin-4(3/-/)-one;
or a pharmaceutically acceptable salt thereof, including all stereoisomers, tautomers and isotopic derivatives thereof.
17. A pharmaceutical composition comprising a compound according to any one of claims 1 to 16, in combination with one or more pharmaceutically acceptable diluents or carriers.
18. A compound of formula (I) according to any one of claims 1 to 16 for use as a medicament.
19. A compound of formula (I) according to any one of claims 1 to 16 for use as in the treatment or prevention of a condition selected from:
COPD (including chronic bronchitis and emphysema), asthma, paediatric asthma, cystic fibrosis, sarcoidosis, idiopathic pulmonary fibrosis, allergic rhinitis, rhinitis, sinusitis, allergic conjunctivitis, conjunctivitis, allergic dermatitis, contact dermatitis, psoriasis, ulcerative colitis, inflamed joints secondary to rheumatoid arthritis or osteoarthritis, rheumatoid arthritis, pancreatitis, cachexia, inhibition of the growth and metastasis of tumours including non-small cell lung carcinoma, breast carcinoma, gastric carcinoma, colorectal carcinomas and malignant melanoma.
20. Use of a compound of formula (I) according to any one of claims 1 to 16 for the manufacture of a medicament for the treatment or prevention of a condition selected from
COPD (including chronic bronchitis and emphysema), asthma, paediatric asthma, cystic fibrosis, sarcoidosis, idiopathic pulmonary fibrosis, allergic rhinitis, rhinitis, sinusitis, allergic conjunctivitis, conjunctivitis, allergic dermatitis, contact dermatitis, psoriasis, ulcerative colitis, inflamed joints secondary to rheumatoid arthritis or osteoarthritis, rheumatoid arthritis, pancreatitis, cachexia, inhibition of the growth and metastasis of tumours including non-small cell lung carcinoma, breast carcinoma, gastric carcinoma, colorectal carcinomas and malignant melanoma.
21 . A method of treatment of a condition selected from
COPD (including chronic bronchitis and emphysema), asthma, paediatric asthma, cystic fibrosis, sarcoidosis, idiopathic pulmonary fibrosis, allergic rhinitis, rhinitis, sinusitis, allergic conjunctivitis, conjunctivitis, allergic dermatitis, contact dermatitis, psoriasis, ulcerative colitis, inflamed joints secondary to rheumatoid arthritis or osteoarthritis, rheumatoid arthritis, pancreatitis, cachexia, inhibition of the growth and metastasis of tumours including non-small cell lung carcinoma, breast carcinoma, gastric carcinoma, colorectal carcinomas and malignant melanoma
which comprises administering to a subject an effective amount of a compound of formula (I) according to any one of claims 1 to 16 or a pharmaceutical composition according to claim 17.
22. An intermediate of formula (VI):
Figure imgf000100_0001
PCT/EP2010/065746 2009-10-19 2010-10-19 Compounds WO2011048111A1 (en)

Priority Applications (60)

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ES10771416.4T ES2477270T3 (en) 2009-10-19 2010-10-19 Quinazolin-4 (3H) -one derivatives used as PI3 kinase inhibitors
MX2012004525A MX2012004525A (en) 2009-10-19 2010-10-19 Compounds.
CN201080047955.2A CN102712645B (en) 2009-10-19 2010-10-19 Compound
SI201030660T SI2491037T1 (en) 2009-10-19 2010-10-19 Quinazolin-4(3h)-one derivatives used as pi3 kinase inhibitors
NZ599162A NZ599162A (en) 2009-10-19 2010-10-19 Compounds having Phosphoinositide 3-kinase Inhibitor Activity
JP2012534671A JP5889792B2 (en) 2009-10-19 2010-10-19 Compound
PL10771416T PL2491037T3 (en) 2009-10-19 2010-10-19 Quinazolin-4(3h)-one derivatives used as pi3 kinase inhibitors
US13/502,465 US8741909B2 (en) 2009-10-19 2010-10-19 PI3 kinase inhibitors
CA2777902A CA2777902C (en) 2009-10-19 2010-10-19 Compounds with p13 kinase inhibitor activity
DK10771416.4T DK2491037T3 (en) 2009-10-19 2010-10-19 QUINAZOLIN-4 (3H) -ONDERIVATIVES USED AS PI3-KINASE INHIBITORS
UAA201203872A UA108743C2 (en) 2009-10-19 2010-10-19 HINAZOLINE-4 (3H) -ONE DERIVATIVES FOR APPLICATION AS RI-KINASE INHIBITORS
BR112012009214A BR112012009214A2 (en) 2009-10-19 2010-10-19 compounds
KR1020127012826A KR101770616B1 (en) 2009-10-19 2010-10-19 Compouds
RS20140344A RS53385B (en) 2009-10-19 2010-10-19 Quinazolin-4(3h)-one derivatives used as pi3 kinase inhibitors
EP10771416.4A EP2491037B1 (en) 2009-10-19 2010-10-19 Quinazolin-4(3h)-one derivatives used as pi3 kinase inhibitors
EA201270575A EA020330B3 (en) 2009-10-19 2010-10-19 Quinazoline compounds
AU2010309832A AU2010309832B2 (en) 2009-10-19 2010-10-19 Compounds
SG2013017470A SG188958A1 (en) 2010-10-18 2011-10-18 Quinazolin-4 (3h) -one derivatives used as pi3 kinase inhibitors
KR1020137011430A KR101857000B1 (en) 2010-10-18 2011-10-18 Quinazolin-4 (3h) -one derivatives used as pi3 kinase inhibitors
EA201390579A EA026946B1 (en) 2010-10-18 2011-10-18 Quinazolin-4(3h)-one derivatives used as pi3 kinase inhibitors
BR112013009395A BR112013009395A2 (en) 2010-10-18 2011-10-18 quinazolin-4 (3h) -one derivatives used as pi3 kinase inhibitors
PCT/GB2011/052015 WO2012052753A1 (en) 2010-10-18 2011-10-18 Quinazolin-4 (3h) -one derivatives used as pi3 kinase inhibitors
PL11787742T PL2630149T3 (en) 2010-10-18 2011-10-18 Quinazolin-4(3h)-one derivatives used as pi3 kinase inhibitors
DK11787742T DK2630149T3 (en) 2010-10-18 2011-10-18 Quinazolin-4 (3H) -one derivatives, USED AS PI 3-kinase inhibitors
EP11787742.3A EP2630149B1 (en) 2010-10-18 2011-10-18 Quinazolin-4(3h)-one derivatives used as pi3 kinase inhibitors
CA2811171A CA2811171A1 (en) 2010-10-18 2011-10-18 Quinazolin-4 (3h) -one derivatives used as pi3 kinase inhibitors
PT117877423T PT2630149E (en) 2010-10-18 2011-10-18 Quinazolin-4(3h)-one derivatives used as pi3 kinase inhibitors
SI201130442T SI2630149T1 (en) 2010-10-18 2011-10-18 Quinazolin-4(3h)-one derivatives used as pi3 kinase inhibitors
NZ609147A NZ609147A (en) 2009-10-19 2011-10-18 Quinazolin-4 (3h) -one derivatives used as pi3 kinase inhibitors
MX2013004298A MX346336B (en) 2010-10-18 2011-10-18 Quinazolin-4 (3h) -one derivatives used as pi3 kinase inhibitors.
RS20150206A RS53898B1 (en) 2010-10-18 2011-10-18 Quinazolin-4(3h)-one derivatives used as pi3 kinase inhibitors
MYPI2013700611A MY164731A (en) 2009-10-19 2011-10-18 Compound
JP2013533289A JP5422783B2 (en) 2010-10-18 2011-10-18 Quinazolin-4 (3H) -one derivatives for use as PI3 kinase inhibitors
AU2011317334A AU2011317334B2 (en) 2010-10-18 2011-10-18 Quinazolin-4(3H)-one derivatives used as PI3 kinase inhibitors
ES11787742.3T ES2533570T3 (en) 2010-10-18 2011-10-18 Quinazolin-4 (3H) -one derivatives, which are used as inhibitors of PI3 kinases
MEP-2015-43A ME02064B (en) 2009-10-19 2011-10-18 Quinazolin-4(3h)-one derivatives used as pi3 kinase inhibitors
PE2013000869A PE20131464A1 (en) 2010-10-18 2011-10-18 QUINAZOLIN-4 (3H) -ONE DERIVATIVES USED AS PI3 KINASE INHIBITORS
IL219248A IL219248A (en) 2009-10-19 2012-04-18 2-[(4-amino-3-hydroxyphenyl-1h-pyrazolo[3,4-c]pyrimidin-1-yl)methyl]- 5-(propynyl)quinazolin-4(3h)-one derivatives, intermediates in the process for their preparation and pharmaceutical compositions comprising said derivatives
ZA2012/03662A ZA201203662B (en) 2009-10-19 2012-05-18 Compounds
HK13100837.2A HK1173728A1 (en) 2009-10-19 2013-01-18 Quinazolin-4(3h)-one derivatives used as pi3 kinase inhibitors pi3 -4(3h)-
IL225212A IL225212A (en) 2010-10-18 2013-03-14 6-(2-((4-amino-3-(3-hydroxyphenyl)-1h-pyrazolo[3,4-d]pyrimidin-1-yl)methyl)-3-(2-chlorobenzyl)-4-oxo-3,4-dihydroquinazolin-5-yl)-n,n- bis(2-methoxyethyl)hex-5-ynamide, pharmaceutical compositions comprising it and uses thereof
GT201300097A GT201300097A (en) 2010-10-18 2013-04-11 DERIVATIVES OF QUINAZOLIN-4 (3H) -ONA USED AS INHIBITORS OF P13 CINASA
NI201300035A NI201300035A (en) 2009-10-19 2013-04-16 DERIVATIVES OF QUINAZOLIN - 4 (3H) - ONA USED AS INHIBITORS OF PI3 KINASE
CL2013001057A CL2013001057A1 (en) 2010-10-18 2013-04-17 A compound 6- (2 - ((4-amino-3- (3-hydroxyphenyl) -1h-pyrazolo [3,4-d] pyrimidin-1-yl) methyl) -3- (2-chlorobenzyl) -4- oxo-3,4-dihydroquinazolin-5-yl) -n, n-bis (2-methoxyethyl) hex-5-inamide, pi3 kinase inhibitors; intermediate compound; pharmaceutical composition, useful to treat or prevent epoc, rhinitis, rheumatoid arthritis and carcinomas.
US13/865,379 US9340545B2 (en) 2010-10-18 2013-04-18 Quinazolin-4 (3H)—one derivatives used as P13 kinase inhibitors
CO13106722A CO6690816A2 (en) 2010-10-18 2013-04-26 Quinazolin-4 (3h) -one derivatives used as inhibitors of pi3 kinase
ECSP13012612 ECSP13012612A (en) 2010-10-18 2013-05-08 DERIVATIVES OF QUINAZOLIN-4- (3H) -ONA USED AS INHIBITORS OF P13 CINASA
ZA2013/03614A ZA201303614B (en) 2010-10-18 2013-05-17 Quinazolin-4(3h)-one derivatives used as p13 kinase inhibitors
CR20130233A CR20130233A (en) 2010-10-18 2013-05-20 DERIVATIVES THAT QUINAZOLIN-4 (3H) -ONA USED AS PI3 CINAZA INHIBITORS
HK13111905.6A HK1184461A1 (en) 2010-10-18 2013-10-23 Quinazolin-4 (3h) -one derivatives used as pi3 kinase inhibitors pi3 -4 (3h)-
US14/220,650 US9321773B2 (en) 2009-10-19 2014-03-20 Compounds
HRP20140599AT HRP20140599T1 (en) 2009-10-19 2014-06-26 Quinazolin-4(3h)-one derivatives used as pi3 kinase inhibitors
SM201400086T SMT201400086B (en) 2009-10-19 2014-06-30 Quinazolin-4 (3H) -one derivatives used as PI3 kinase inhibitors
HRP20150318TT HRP20150318T1 (en) 2010-10-18 2015-03-19 Quinazolin-4 (3h) -one derivatives used as pi3 kinase inhibitors
CY20151100287T CY1116315T1 (en) 2010-10-18 2015-03-23 KINAZOLIN-4 (3H) -ONE DERIVATIVES USED AS RI3
SM201500076T SMT201500076B (en) 2010-10-18 2015-03-23 Quinazolin-4 (3H) -one derivatives used as PI3 kinase inhibitors
US15/086,120 US9834560B2 (en) 2009-10-19 2016-03-31 Compounds
US15/092,707 US9637494B2 (en) 2010-10-18 2016-04-07 Quinazolin-4 (3H)-one derivatives used as P13 kinase inhibitors
US15/474,059 US10028959B2 (en) 2010-10-18 2017-03-30 Quinazolin-4 (3H)-one derivatives used as P13 kinase inhibitors
US15/806,742 US20180118754A1 (en) 2009-10-19 2017-11-08 Compounds

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Cited By (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012052753A1 (en) * 2010-10-18 2012-04-26 Respivert Limited Quinazolin-4 (3h) -one derivatives used as pi3 kinase inhibitors
WO2013088404A1 (en) 2011-12-15 2013-06-20 Novartis Ag Use of inhibitors of the activity or function of PI3K
WO2013136076A1 (en) 2012-03-13 2013-09-19 Respivert Limited Crystalline pi3 kinase inhibitors
WO2014072937A1 (en) 2012-11-08 2014-05-15 Rhizen Pharmaceuticals Sa Pharmaceutical compositions containing a pde4 inhibitor and a pi3 delta or dual pi3 delta-gamma kinase inhibitor
WO2014140597A1 (en) 2013-03-15 2014-09-18 Respivert Limited 2-((4-amino-3-(3-fluoro-5-hydroxyphenyl)-1h-pyrazolo[3,4-d]pyrimidin-1 -yl)methyl)-3-(2-(trifluoromet hyl)benzyl)quinazolin-4(3h)-one derivatives and their use as phosphoinositide 3-kinase inhibitors
WO2014198852A2 (en) 2013-06-14 2014-12-18 Psioxus Theraupeutics Limited A dosing regime and formulations for type b adenoviruses
US9227977B2 (en) 2013-03-15 2016-01-05 Respivert Ltd. Phosphoinositide 3-kinase inhibitors
US9255108B2 (en) 2012-04-10 2016-02-09 Infinity Pharmaceuticals, Inc. Heterocyclic compounds and uses thereof
US9321773B2 (en) 2009-10-19 2016-04-26 Respivert, Ltd. Compounds
US9359365B2 (en) 2013-10-04 2016-06-07 Infinity Pharmaceuticals, Inc. Heterocyclic compounds and uses thereof
WO2016097158A1 (en) 2014-12-17 2016-06-23 Psioxus Therapeutics Limited Method of treating ovarian cancer
US9540372B2 (en) 2007-12-19 2017-01-10 Institute Of Cancer Research: Royal Cancer Hospital (The) Pyrido[2,3-b]pyrazin-8-substituted compounds and their use
US9708348B2 (en) 2014-10-03 2017-07-18 Infinity Pharmaceuticals, Inc. Trisubstituted bicyclic heterocyclic compounds with kinase activities and uses thereof
US9708317B2 (en) 2013-11-25 2017-07-18 Cancer Research Technology Limited Process for the preparation of 8-(4-aminophenoxy)-4H-pyrido[2,3-B]pyrazin-3-one derivatives
US9725447B2 (en) 2013-11-25 2017-08-08 Cancer Research Technology Limited 1-(5-tert-butyl-2-aryl-pyrazol-3-yl)-3-[2-fluoro-4-[(3-oxo-4H-pyrido[2,3-b]pyrazin-8-yl)oxy]phenyl]urea derivatives as RAF inhibitors for the treatment of cancer
US9751888B2 (en) 2013-10-04 2017-09-05 Infinity Pharmaceuticals, Inc. Heterocyclic compounds and uses thereof
US9775844B2 (en) 2014-03-19 2017-10-03 Infinity Pharmaceuticals, Inc. Heterocyclic compounds and uses thereof
US9820976B2 (en) 2010-02-01 2017-11-21 Cancer Research Technology Limited 1-(5-tert-butyl-2-phenyl-2H-pyrazol-3-yl)-3-[2-fluoro-4-(1-methyl-2-oxo-2,3-dihydro-1H-imidazo[4,5-b]pyridin-7-yloxy)-phenyl]-urea and related compounds and their use in therapy
US9944639B2 (en) 2014-07-04 2018-04-17 Lupin Limited Quinolizinone derivatives as PI3K inhibitors
US10160761B2 (en) 2015-09-14 2018-12-25 Infinity Pharmaceuticals, Inc. Solid forms of isoquinolinones, and process of making, composition comprising, and methods of using the same
WO2020058072A1 (en) * 2018-09-18 2020-03-26 Société des Produits Nestlé S.A. Src inhibitor compounds for skeletal muscle modulation, methods and uses thereof
US10759806B2 (en) 2016-03-17 2020-09-01 Infinity Pharmaceuticals, Inc. Isotopologues of isoquinolinone and quinazolinone compounds and uses thereof as PI3K kinase inhibitors
US10919914B2 (en) 2016-06-08 2021-02-16 Infinity Pharmaceuticals, Inc. Heterocyclic compounds and uses thereof

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10751339B2 (en) 2018-01-20 2020-08-25 Sunshine Lake Pharma Co., Ltd. Substituted aminopyrimidine compounds and methods of use
CN108516976B (en) * 2018-04-27 2020-07-03 四川大学华西医院 7-deazapurine derivative and four-membered ring supramolecular structure thereof
CN114258393A (en) * 2020-07-21 2022-03-29 中国医药研究开发中心有限公司 Heterocyclic compound with dual phosphatidylinositol 3-kinase delta and gamma inhibitor activity and medical application thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001081346A2 (en) 2000-04-25 2001-11-01 Icos Corporation Inhibitors of human phosphatidyl-inositol 3-kinase delta
WO2005113556A1 (en) * 2004-05-13 2005-12-01 Icos Corporation Quinazolinones as inhibitors of human phosphatidylinositol 3-kinase delta
WO2007114926A2 (en) * 2006-04-04 2007-10-11 The Regents Of The University Of California Kinase antagonists

Family Cites Families (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1204826B (en) 1986-03-04 1989-03-10 Chiesi Farma Spa INHALATION PHARMACEUTICAL COMPOSITIONS
GB9501841D0 (en) 1995-01-31 1995-03-22 Co Ordinated Drug Dev Improvements in and relating to carrier particles for use in dry powder inhalers
CA2347856C (en) 1998-11-13 2009-02-17 Jago Research Ag Dry powder for inhalation
EP1140938B1 (en) 1999-01-11 2003-08-27 Princeton University High affinity inhibitors for target validation and uses thereof
PT1158958E (en) 1999-03-05 2007-08-13 Chiesi Farma Spa Improved powdery pharmaceutical compositions for inhalation
PE20011227A1 (en) 2000-04-17 2002-01-07 Chiesi Farma Spa PHARMACEUTICAL FORMULATIONS FOR DRY POWDER INHALERS IN THE FORM OF HARD AGGLOMERATES
US6667300B2 (en) * 2000-04-25 2003-12-23 Icos Corporation Inhibitors of human phosphatidylinositol 3-kinase delta
US6608053B2 (en) 2000-04-27 2003-08-19 Yamanouchi Pharmaceutical Co., Ltd. Fused heteroaryl derivatives
ES2360933T3 (en) 2000-04-27 2011-06-10 Astellas Pharma Inc. CONDENSED HETEROARILO DERIVATIVES.
EP1217000A1 (en) 2000-12-23 2002-06-26 Aventis Pharma Deutschland GmbH Inhibitors of factor Xa and factor VIIa
JP2005509594A (en) 2001-07-13 2005-04-14 バーチャル ドラッグ ディヴェロップメント、インコーポレイティッド NAD synthetase inhibitors and uses thereof
AU2002317377A1 (en) 2001-07-20 2003-03-03 Cancer Research Technology Limited Biphenyl apurinic/apyrimidinic site endonuclease inhibitors to treat cancer
WO2004037176A2 (en) 2002-10-21 2004-05-06 Bristol-Myers Squibb Company Quinazolinones and derivatives thereof as factor xa inhibitors
MXPA05009771A (en) 2003-03-14 2005-10-26 Ono Pharmaceutical Co Nitrogen-containing heterocyclic derivatives and drugs containing the same as the active ingredient.
US7122557B2 (en) 2003-03-18 2006-10-17 Bristol-Myers Squibb Company Sulfonyl-amidino-containing and tetrahydropyrimidino-containing compounds as factor Xa inhibitors
US20050002930A1 (en) 2003-04-03 2005-01-06 Johnson Dale E. Methods of production and use of anti-integrin antibodies for the control of tissue granulation
US7429596B2 (en) 2003-06-20 2008-09-30 The Regents Of The University Of California 1H-pyrrolo [2,3-D] pyrimidine derivatives and methods of use thereof
GB0316341D0 (en) 2003-07-11 2003-08-13 Glaxo Group Ltd Pharmaceutical formulations
EP1661879A4 (en) 2003-08-04 2006-11-29 Ono Pharmaceutical Co Diphenyl ether compound, process for producing the same, and use
WO2005016348A1 (en) 2003-08-14 2005-02-24 Icos Corporation Method of inhibiting immune responses stimulated by an endogenous factor
US20050054614A1 (en) 2003-08-14 2005-03-10 Diacovo Thomas G. Methods of inhibiting leukocyte accumulation
GB0326632D0 (en) 2003-11-14 2003-12-17 Jagotec Ag Dry powder formulations
WO2005067901A2 (en) 2004-01-08 2005-07-28 Michigan State University Methods for treating and preventing hypertension and hypertension-related disorders
CA2566436C (en) 2004-05-13 2011-05-10 Vanderbilt University Phosphoinositide 3-kinase delta selective inhibitors for inhibiting angiogenesis
CA2567883A1 (en) 2004-05-25 2005-12-15 Icos Corporation Methods for treating and/or preventing aberrant proliferation of hematopoietic cells
CN101123968A (en) 2004-06-04 2008-02-13 艾科斯有限公司 Methods for treating mast cell disorders
AU2005283326B2 (en) 2004-09-13 2011-07-21 Ono Pharmaceutical Co., Ltd. Nitrogenous heterocyclic derivative and medicine containing the same as an active ingredient
GB0425758D0 (en) 2004-11-23 2004-12-22 Vectura Ltd Preparation of pharmaceutical compositions
WO2006089106A2 (en) 2005-02-17 2006-08-24 Icos Corporation Phosphoinositide 3-kinase inhibitors for inhibiting leukocyte accumulation
GB0525254D0 (en) 2005-12-12 2006-01-18 Jagotec Ag Powder compositions for inhalation
CN101448505A (en) * 2006-04-04 2009-06-03 加利福尼亚大学董事会 P13 kinase antagonists
CN101495515A (en) 2006-05-24 2009-07-29 拜耳先灵医药股份有限公司 High affinity human and humanized anti-alpha5beta1 integrin function blocking antibodies with reduced immunogenicity
CA2655720A1 (en) 2006-06-29 2008-01-10 Schering Corporation Substituted bicyclic and tricyclic thrombin receptor antagonists
GB0622818D0 (en) 2006-11-15 2006-12-27 Jagotec Ag Improvements in or relating to organic compounds
CA2668744C (en) 2006-11-17 2015-09-15 Queen's University At Kingston Compounds and methods for treating protein folding disorders
WO2008067219A2 (en) 2006-11-29 2008-06-05 Kalypsys, Inc. Quinazolinone modulators of tgr5
US8338437B2 (en) 2007-02-28 2012-12-25 Methylgene Inc. Amines as small molecule inhibitors
JP2010526120A (en) * 2007-05-09 2010-07-29 ノバルティス アーゲー Substituted imidazopyridazines as PI3K lipid kinase inhibitors
WO2008140750A1 (en) 2007-05-10 2008-11-20 Hydra Biosciences Inc. Compounds for modulating trpv3 function
US8703777B2 (en) 2008-01-04 2014-04-22 Intellikine Llc Certain chemical entities, compositions and methods
JP5731978B2 (en) 2008-09-26 2015-06-10 インテリカイン， エルエルシー Heterocyclic kinase inhibitor
WO2010059593A1 (en) 2008-11-18 2010-05-27 Intellikine, Inc. Methods and compositions for treatment of ophthalmic conditions
WO2010065923A2 (en) 2008-12-04 2010-06-10 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Phosphatidylinositol-3-kinase p110 delta-targeted drugs in the treatment of cns disorders
EP2379505A4 (en) 2008-12-05 2012-10-17 Designmedix Inc Modified chloroquines with single ring moiety or fused ring moiety
US20110135655A1 (en) * 2009-01-13 2011-06-09 PHILADELPHIA HEALTH AND EDUCATION CORPORATION d/b/a Drexel University College of Medicine; Role of PI3K p110 delta Signaling in Retroviral Infection and Replication
EP2411391A1 (en) * 2009-03-24 2012-02-01 Gilead Calistoga LLC Atropisomers of2-purinyl-3-tolyl-quinazolinone derivatives and methods of use
AU2010281265A1 (en) 2009-08-05 2012-03-22 Versitech Limited Antiviral compounds and methods of making and using thereof
GB0918249D0 (en) 2009-10-19 2009-12-02 Respivert Ltd Compounds
WO2012041717A1 (en) 2010-09-30 2012-04-05 Chiesi Farmaceutici S.P.A. Use of magnesium stearate in dry powder formulations for inhalation
UY33337A (en) * 2010-10-18 2011-10-31 Respivert Ltd SUBSTITUTED DERIVATIVES OF 1H-PIRAZOL [3,4-d] PYRIMIDINE AS INHIBITORS OF PHOSFOINOSITIDE 3-KINASES
MX355299B (en) 2012-03-13 2018-04-11 Respivert Ltd Crystalline pi3 kinase inhibitors.

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001081346A2 (en) 2000-04-25 2001-11-01 Icos Corporation Inhibitors of human phosphatidyl-inositol 3-kinase delta
WO2005113556A1 (en) * 2004-05-13 2005-12-01 Icos Corporation Quinazolinones as inhibitors of human phosphatidylinositol 3-kinase delta
WO2007114926A2 (en) * 2006-04-04 2007-10-11 The Regents Of The University Of California Kinase antagonists
WO2008127226A2 (en) 2006-04-04 2008-10-23 The Regents Of The University Of California P13 kinase antagonists

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
CLAYDON, J. ET AL., ANGEW. CHEM. INT. ED., vol. 48, 2009, pages 6398 - 6401
DOUKAS, J. ET AL., J PHARMACOL. EXP. THER., vol. 328, 2009, pages 758 - 765
ITO, K. ET AL., J PHARMACOL. EXP. THER., vol. 321, 2007, pages 1 - 8
LEE, K. S. ET AL., FASEB J., vol. 20, 2006, pages 455 - 65
LEE, K. S. ET AL., J. ALLERGY CLIN. IMMUNOL., vol. 118, 2006, pages 403 - 409
MEDICHERLA, S. ET AL., J. PHARMACOL. EXP. THER., vol. 324, 2008, pages 921 - 9
RAMEH, L. E.; CANTLEY, L. C., J. BIOL. CHEM., vol. 274, 1999, pages 8347 - 8350
SADHU, C. ET AL., BIOCHEM. BIOPHYS. RES. COMMUN., vol. 308, 2003, pages 764 - 9
TO, Y. ET AL., AM. J. RESPIR. CRIT. CARE MED., vol. 182, 2010, pages 897 - 904

Cited By (59)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9540372B2 (en) 2007-12-19 2017-01-10 Institute Of Cancer Research: Royal Cancer Hospital (The) Pyrido[2,3-b]pyrazin-8-substituted compounds and their use
US9321773B2 (en) 2009-10-19 2016-04-26 Respivert, Ltd. Compounds
US9834560B2 (en) 2009-10-19 2017-12-05 Respivert Ltd. Compounds
US9820976B2 (en) 2010-02-01 2017-11-21 Cancer Research Technology Limited 1-(5-tert-butyl-2-phenyl-2H-pyrazol-3-yl)-3-[2-fluoro-4-(1-methyl-2-oxo-2,3-dihydro-1H-imidazo[4,5-b]pyridin-7-yloxy)-phenyl]-urea and related compounds and their use in therapy
AU2011317334B2 (en) * 2010-10-18 2015-06-11 Respivert Limited Quinazolin-4(3H)-one derivatives used as PI3 kinase inhibitors
US9637494B2 (en) 2010-10-18 2017-05-02 Respivert, Ltd. Quinazolin-4 (3H)-one derivatives used as P13 kinase inhibitors
EA026946B1 (en) * 2010-10-18 2017-06-30 Респайверт Лимитед Quinazolin-4(3h)-one derivatives used as pi3 kinase inhibitors
US10028959B2 (en) 2010-10-18 2018-07-24 Respivert Ltd. Quinazolin-4 (3H)-one derivatives used as P13 kinase inhibitors
WO2012052753A1 (en) * 2010-10-18 2012-04-26 Respivert Limited Quinazolin-4 (3h) -one derivatives used as pi3 kinase inhibitors
US9340545B2 (en) 2010-10-18 2016-05-17 Respivert Ltd. Quinazolin-4 (3H)—one derivatives used as P13 kinase inhibitors
WO2013088404A1 (en) 2011-12-15 2013-06-20 Novartis Ag Use of inhibitors of the activity or function of PI3K
JP2018008992A (en) * 2012-03-13 2018-01-18 レスピバート・リミテツド Crystalline pi3 kinase inhibitor
CN104302650B (en) * 2012-03-13 2017-07-18 瑞斯比维特有限公司 Crystal pi3 kinase inhibitors
JP2015509973A (en) * 2012-03-13 2015-04-02 レスピバート・リミテツド Crystalline PI3 kinase inhibitor
US9642799B2 (en) 2012-03-13 2017-05-09 Respivert, Ltd. Crystalline 6-(2-((4-amino-3-(3-hydroxyphenyl)-1H-pyrazolo[3,4-D]pyrimidin-1-yl)methyl)-3-(2-chlorobenzyl)-4-0X0-3,4-dihydroquinazolin-5-yl)-N,N-bis(2-methoxyethyl)hex-5-ynamide
JP2015509972A (en) * 2012-03-13 2015-04-02 レスピバート・リミテツド New pharmaceutical formulation
CN104302650A (en) * 2012-03-13 2015-01-21 瑞斯比维特有限公司 Crystalline pi3 kinase inhibitors
CN104284679A (en) * 2012-03-13 2015-01-14 瑞斯比维特有限公司 Novel pharmaceutical formulations
TWI586378B (en) * 2012-03-13 2017-06-11 瑞斯比維特有限公司 Novel pharmaceutical formulations
EA023715B1 (en) * 2012-03-13 2016-07-29 Респайверт Лимитед Formulation for use in treatment of pi3 kinase mediated diseases
EA025465B1 (en) * 2012-03-13 2016-12-30 Респайверт Лимитед METHOD OF INCREASING THE STABILITY TO CHEMICAL DEGRADATION OF A PHARMACEUTICAL FORMULATION COMPRISING 6-(2-((4-AMINO-3-(3-HYDROXYPHENYL)-1H-PYRAZOLO[3,4-d]PYRIMIDIN-1-YL)METHYL)-3-(2-CHLOROBENZYL)-4-OXO-3,4-DIHYDROQYINAZOLIN-5-YL)-N,N-BIS(2-METHOXYETHYL)HEX-5-YNAMIDE
WO2013136076A1 (en) 2012-03-13 2013-09-19 Respivert Limited Crystalline pi3 kinase inhibitors
WO2013136075A1 (en) 2012-03-13 2013-09-19 Respivert Limited Novel pharmaceutical formulations
AU2013234081B2 (en) * 2012-03-13 2017-02-02 Respivert Limited Crystalline PI3 kinase inhibitors
US9255108B2 (en) 2012-04-10 2016-02-09 Infinity Pharmaceuticals, Inc. Heterocyclic compounds and uses thereof
WO2014072937A1 (en) 2012-11-08 2014-05-15 Rhizen Pharmaceuticals Sa Pharmaceutical compositions containing a pde4 inhibitor and a pi3 delta or dual pi3 delta-gamma kinase inhibitor
EA029218B1 (en) * 2013-03-15 2018-02-28 Респайверт Лимитед 2-((4-AMINO-3-(3-FLUORO-5-HYDROXYPHENYL)-1H-PYRAZOLO[3,4-d]PYRIMIDIN-1-YL)METHYL)-3-(2-(TRIFLUOROMETHYL)BENZYL)QUINAZOLIN-4(3H)-ONE DERIVATIVES AND THEIR USE AS PHOSPHOINOSITIDE 3-KINASE INHIBITORS
AU2014229768B2 (en) * 2013-03-15 2018-01-04 Respivert Limited 2-((4-amino-3-(3-fluoro-5-hydroxyphenyl)-1H-pyrazolo[3,4-d]pyrimidin-1 -yl)methyl)-3-(2-(trifluoromet hyl)benzyl)quinazolin-4(3H)-one derivatives and their use as phosphoinositide 3-kinase inhibitors
WO2014140597A1 (en) 2013-03-15 2014-09-18 Respivert Limited 2-((4-amino-3-(3-fluoro-5-hydroxyphenyl)-1h-pyrazolo[3,4-d]pyrimidin-1 -yl)methyl)-3-(2-(trifluoromet hyl)benzyl)quinazolin-4(3h)-one derivatives and their use as phosphoinositide 3-kinase inhibitors
US9227977B2 (en) 2013-03-15 2016-01-05 Respivert Ltd. Phosphoinositide 3-kinase inhibitors
CN105229007A (en) * 2013-03-15 2016-01-06 瑞斯比维特有限公司 2-((4-amino-3-(the fluoro-5-hydroxy phenyl of 3-)-1H-pyrazolo [3,4-D] pyrimidine-1-base) methyl)-3-(2-(trifluoromethyl) benzyl) quinazoline-4 (3H)-one derivative and its purposes as phosphoinositide 3-kinase inhibitor
US9556184B2 (en) 2013-03-15 2017-01-31 Respivert, Ltd. Phosphoinositide 3-kinase inhibitors
CN105229007B (en) * 2013-03-15 2017-08-25 瑞斯比维特有限公司 2 ((base of 4 amino 3 (hydroxy phenyl of 3 fluorine 5) 1H pyrazolos [3,4 D] pyrimidine 1) methyl) 3 (2 (trifluoromethyl) benzyl) (3H) ketone derivatives of quinazoline 4 and its purposes as the kinase inhibitor of phosphoinositide 3
US9745306B2 (en) 2013-03-15 2017-08-29 Respivert Limited 2-((4-amino-3-(3-fluoro-5-hydroxyphenyl)-1H-pyrazolo[3,4-D]pyrimidin-1-yl)methyl)-3-(2-(trifluoromethyl)benzyl) quinazolin-4(3H)-one derivatives and their use as phosphoinositide 3-kinase inhibitors
EP3777870A1 (en) 2013-06-14 2021-02-17 Psioxus Therapeutics Limited A dosing regime and formulations for type b adenoviruses
WO2014198852A2 (en) 2013-06-14 2014-12-18 Psioxus Theraupeutics Limited A dosing regime and formulations for type b adenoviruses
US11173186B2 (en) 2013-06-14 2021-11-16 Psioxus Therapeutics Limited Dosing regime and formulations for type B adenovirus
US9751888B2 (en) 2013-10-04 2017-09-05 Infinity Pharmaceuticals, Inc. Heterocyclic compounds and uses thereof
US9359365B2 (en) 2013-10-04 2016-06-07 Infinity Pharmaceuticals, Inc. Heterocyclic compounds and uses thereof
US9828377B2 (en) 2013-10-04 2017-11-28 Infinity Pharmaceuticals, Inc. Heterocyclic compounds and uses thereof
US10329299B2 (en) 2013-10-04 2019-06-25 Infinity Pharmaceuticals, Inc. Heterocyclic compounds and uses thereof
US9725447B2 (en) 2013-11-25 2017-08-08 Cancer Research Technology Limited 1-(5-tert-butyl-2-aryl-pyrazol-3-yl)-3-[2-fluoro-4-[(3-oxo-4H-pyrido[2,3-b]pyrazin-8-yl)oxy]phenyl]urea derivatives as RAF inhibitors for the treatment of cancer
US10167282B2 (en) 2013-11-25 2019-01-01 Cancer Research Technology Limited 1-(5-tert-butyl-2-aryl-pyrazol-3-yl)-3-[2-fluoro-4-[(3-oxo-4H-pyrido [2, 3-B]pyrazin- 8-yl)oxy]phenyl]urea derivatives as RAF inhibitors for the treatment of cancer
US9708317B2 (en) 2013-11-25 2017-07-18 Cancer Research Technology Limited Process for the preparation of 8-(4-aminophenoxy)-4H-pyrido[2,3-B]pyrazin-3-one derivatives
US10100053B2 (en) 2013-11-25 2018-10-16 Cancer Research Technology Limited Process for the preparation of 8-(4-aminophenoxy)-4H-pyrido[2,3-b]pyrazin-3-one derivatives
US9775844B2 (en) 2014-03-19 2017-10-03 Infinity Pharmaceuticals, Inc. Heterocyclic compounds and uses thereof
US11541059B2 (en) 2014-03-19 2023-01-03 Infinity Pharmaceuticals, Inc. Heterocyclic compounds and uses thereof
US10675286B2 (en) 2014-03-19 2020-06-09 Infinity Pharmaceuticals, Inc. Heterocyclic compounds and uses thereof
US9944639B2 (en) 2014-07-04 2018-04-17 Lupin Limited Quinolizinone derivatives as PI3K inhibitors
US10253047B2 (en) 2014-10-03 2019-04-09 Infinity Pharmaceuticals, Inc. Heterocyclic compounds and uses thereof
US10941162B2 (en) 2014-10-03 2021-03-09 Infinity Pharmaceuticals, Inc. Heterocyclic compounds and uses thereof
US9708348B2 (en) 2014-10-03 2017-07-18 Infinity Pharmaceuticals, Inc. Trisubstituted bicyclic heterocyclic compounds with kinase activities and uses thereof
WO2016097158A1 (en) 2014-12-17 2016-06-23 Psioxus Therapeutics Limited Method of treating ovarian cancer
US11939333B2 (en) 2015-09-14 2024-03-26 Infinity Pharmaceuticals, Inc. Solid forms of isoquinolinones, and process of making, composition comprising, and methods of using the same
US10160761B2 (en) 2015-09-14 2018-12-25 Infinity Pharmaceuticals, Inc. Solid forms of isoquinolinones, and process of making, composition comprising, and methods of using the same
US11247995B2 (en) 2015-09-14 2022-02-15 Infinity Pharmaceuticals, Inc. Solid forms of isoquinolinones, and process of making, composition comprising, and methods of using the same
US10759806B2 (en) 2016-03-17 2020-09-01 Infinity Pharmaceuticals, Inc. Isotopologues of isoquinolinone and quinazolinone compounds and uses thereof as PI3K kinase inhibitors
US10919914B2 (en) 2016-06-08 2021-02-16 Infinity Pharmaceuticals, Inc. Heterocyclic compounds and uses thereof
WO2020058072A1 (en) * 2018-09-18 2020-03-26 Société des Produits Nestlé S.A. Src inhibitor compounds for skeletal muscle modulation, methods and uses thereof

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EA020330B1 (en) 2014-10-30
CR20120264A (en) 2013-01-25
EP2491037B1 (en) 2014-04-30
US9321773B2 (en) 2016-04-26
US9834560B2 (en) 2017-12-05
RS53385B (en) 2014-10-31
SMT201400086B (en) 2014-09-08
DK2491037T3 (en) 2014-07-21
PL2491037T3 (en) 2014-09-30
US20160264583A1 (en) 2016-09-15
BR112012009214A2 (en) 2016-08-16
CY1116057T1 (en) 2017-02-08

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