WO2011037348A2 - Microorganism analyzer, and analysis method using same - Google Patents

Microorganism analyzer, and analysis method using same Download PDF

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Publication number
WO2011037348A2
WO2011037348A2 PCT/KR2010/006266 KR2010006266W WO2011037348A2 WO 2011037348 A2 WO2011037348 A2 WO 2011037348A2 KR 2010006266 W KR2010006266 W KR 2010006266W WO 2011037348 A2 WO2011037348 A2 WO 2011037348A2
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WO
WIPO (PCT)
Prior art keywords
chamber
sample
medium
analysis device
microbial analysis
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Application number
PCT/KR2010/006266
Other languages
French (fr)
Korean (ko)
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WO2011037348A3 (en
Inventor
유재천
Original Assignee
일렉트론 바이오 주식회사
성균관대학교 산학협력단
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Application filed by 일렉트론 바이오 주식회사, 성균관대학교 산학협력단 filed Critical 일렉트론 바이오 주식회사
Publication of WO2011037348A2 publication Critical patent/WO2011037348A2/en
Publication of WO2011037348A3 publication Critical patent/WO2011037348A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/24—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • G01N2333/255—Salmonella (G)

Definitions

  • a chromogenic medium, agar medium, and selective medium having a specific color according to the culture state of a particular bacterium by the biochemical reaction of a sugar in a medium and an enzyme in a microorganism.
  • the present invention relates to a microbial analysis apparatus capable of detecting microorganisms present in food, milk, dairy products, agricultural products, beverages, and environmental samples using any one or more of the selective medium, and an analysis method using the same.
  • the present invention relates to a microbial analysis apparatus capable of counting colonies and an analysis method using the same.
  • the cultures are differentiated by seeing the color of the grown colonies.
  • the microorganism analyzing apparatus of the present invention and an analysis method using the same are suitable for a thin film type apparatus for diagnosing and detecting a small amount of microorganisms present in a sample for contaminants such as food poisoning test, food microbial test, and water quality.
  • the existing microbial analysis process has a problem that not only the data may be wrong due to microbial contamination during these processes due to manual operations such as platinum sterilization and streaking inoculation, but only a highly expert expert can perform this task. .
  • the present invention has been made to solve the above-described problems, the object of the present invention, a sample injection port for injecting a sample; A sampler for collecting a sample from an inspection site and injecting the collected sample into the sample inlet; A prep chamber for temporarily storing a sample injected from the sample inlet; An enrichment chamber which takes a sample from the prep chamber and includes a liquid culture medium for enriching the microorganisms present in the sample; At least one medium chamber for providing nutrients to the enriched microorganism; A trech chamber for collecting debris and excess sample; And a valve for moving fluid and sample between the chambers; A flow path (channel) for providing a movement path of the specimen; And it provides a microbial analysis device having a rotatable body in which the flow path, valve and chamber are integrated and an analysis method using the same.
  • a sample injection port for injecting a sample sample;
  • a sampler for collecting a sample from a test site and including a liquid culture solution for enriching microorganisms present in the sample and injecting the sample into the sample inlet;
  • a prep chamber for temporarily storing a sample injected from the sampler;
  • a medium chamber for taking a sample from the prep chamber and providing nutrients to the microorganisms in the sample;
  • a trech chamber for collecting debris and excess sample;
  • a valve for moving fluid and sample between the chambers;
  • a flow path (channel) for providing a movement path of the specimen;
  • it provides a microbial analysis device having a rotatable body in which the flow path, valve and chamber are integrated and an analysis method using the same.
  • another object of the present invention (a) injecting a sample to the prep chamber using a sampler; (b) transferring the sample in the preparation chamber to the media chamber; (c) applying and inoculating the medium surface in the medium chamber with the sample transferred to the medium chamber; (e) It provides an analysis method using a microbial analysis apparatus according to the present invention comprising a cleaning step of collecting the surplus specimen in the trech chamber after application inoculation.
  • the present invention is a sample injection port for injecting a sample;
  • a sampler for collecting a sample from an inspection site and injecting the collected sample into the sample inlet;
  • a prep chamber for temporarily storing a sample injected from the sample inlet;
  • An enrichment chamber containing a sample from the prep chamber and containing a liquid culture medium for enriching the microorganisms present in the sample;
  • At least one medium chamber for providing nutrients to the enriched microorganism;
  • a trech chamber for collecting debris and excess sample;
  • a valve for moving fluid and sample between the chambers;
  • a flow path (channel) for providing a movement path of the specimen;
  • a rotatable body in which the flow path, the valve and the chamber are integrated.
  • the body is mixed with the disk.
  • the body is composed of an upper body and a lower body and is preferably combined to form a body.
  • the sampler preferably comprises a microbial extract for extracting microorganisms from the collected samples.
  • the sampler is preferably a pipette for sampling a sample incubated with liquid enrichment medium in a sterilization pack.
  • the liquid white liquor or liquid enrichment medium is mEC Broth, RV (Rappaport Vassiliadis) Broth, Listeria Enrichment Broth, UVM-modified Listeria Enrichment Broth, Fraser Broth, Tryptone Sonya Broth (TSB), PBS (phosphate buffered saline) Preference is given to any one selected from among Peptone water, lactose broth and desoxycholate lactose broth.
  • the medium chamber is XLD Agar, MacConkey Agar, Desoxycholate, Citrate Agar, Bismuth Sulfite Agar, Nutrient Agar, TSI Agar, Sorbitol MacConkey Agar, Oxford Agar, PALCAM Agar, LPM Agar, Mannitol Salt Agar, Baird-Parker Agar, MYP Agar, It is preferred to include at least one selected from chromogenic media and solid media.
  • sample inlet for injecting a sample sample
  • a sampler for collecting the sample from the test site and including a liquid culture solution for enriching the microorganisms present in the sample and injecting the sample into the sample inlet
  • a prep chamber for temporarily storing a sample injected from the sampler
  • a medium chamber for taking a sample from the prep chamber and providing nutrients to the microorganisms in the sample
  • a trech chamber for collecting debris and excess sample
  • a valve for moving fluid and sample between the chambers
  • a flow path (channel) for providing a movement path of the specimen
  • a rotatable body in which the flow path, the valve and the chamber are integrated.
  • the body of the present invention may further include a cover means capable of opening and closing allowing identification experiment.
  • This cover means allows the collection of a sample of suspected colonies in the media chamber.
  • identification experiment refers to an experiment in which bacteria are cultured through a medium, samples of suspected colonies are sampled, and confirmed through biochemical, serological and genetic experimental methods.
  • the body of the present invention may further include an oxygen supply valve for supplying oxygen to the aerobic microorganisms.
  • the oxygen supply valve is closed during the distribution period, and is opened at the start of the culture, thereby preventing the drying of the medium in the media chamber during the distribution period and supplying oxygen to the aerobic microorganisms during the culture.
  • the solid medium contains a large amount of water. Therefore, if cultured straight, evaporation can occur, and the upper body ceiling may be moist and humid, and the culture state of the microorganisms may be damaged, and the medium may dry out immediately during the culture.
  • the medium in the medium chamber of the present invention is preferably a solid medium, in which case the medium is installed on the body side.
  • the microorganism analyzing apparatus of the present invention preferably includes a microscopic sensor for observing microorganisms and a central control unit for processing images from the microscopic sensors and counting colonies.
  • Microbial culture usually requires a culture time of 24 to 48 hours. However, by viewing the microorganisms in the medium chamber under a microscope sensor, not only can the microorganisms grow earlier, but also the survival of the strains can be confirmed by changing the size of colonies over time. As colonies grow in size over time, the living colony can be considered a living cell.
  • the microscope sensor has an optical zoom of 20 to 100 times.
  • the sampler of the present invention may further comprise a homogenizing means.
  • the microbial analysis device is characterized in that it further comprises a temperature control device to a thermostat for providing optimum conditions of microbial enrichment.
  • the microbial extract or diluent is preferably distilled water (DeIonize water, DI water), PBS (Phosphate Buffered Saline).
  • the liquid culture solution is preferably peptone water, meat extract, yeast extract.
  • the body of the microbial analysis apparatus of the present invention is preferably a disk such as a conventional CD-ROM, DVD and the like.
  • the thickness of the body is preferably 2mm to 100mm, the diameter of 30mm ⁇ 120mm is preferred, the round disk of 120mm, 80mm, or 32mm is preferred.
  • the medium chamber is composed of a selective medium that selectively reacts with different types of microorganisms, and therefore, it is preferable to simultaneously analyze a plurality of microorganisms.
  • the medium chamber is composed of one or more medium selected from phlorogenic medium, chromogenic medium, differentiation medium, selection medium, characterized in that for analyzing a plurality of microorganisms at the same time.
  • the "selective medium” is a medium used for selectively cultivating only desired microorganisms by promoting the growth of specific microorganisms and inhibiting the growth of other microorganisms, for example, MacConkey Agar medium, Salmonella-shigella medium and the like. Can be used.
  • the "fluorogenic medium” refers to a medium that exhibits a unique fluorescent light depending on the culture state of a specific bacterium by the biochemical reaction of a sugar in a medium and an enzyme in a microorganism.
  • Microbial analysis apparatus is Salmonella (Salmonella), Bacillus (Bacillus), Listeria, Vibrio, Campylobacter S. aureus, E. Coliform, E. coli, E. coli, Shigella, Legionella Enterobacter sakazakii, Citrobacter, Preteus, Citrobacter, MRSA, E.coli O157, E.coli spp, L.monocytogenes, L.inocua, V.parahaemolyticus, V.vulnificus & V.cholerae, V.alginolyticus, Coliforms, Pseudomonas Microorganisms such as Klebsiella, Citrobacter, Enterococcus, PMP group, Staphylococcus saprophyticus, Stapylococcus aureus, S. agalactiae, Candida are preferred.
  • microorganisms have a unique color depending on the culture state of specific bacteria by colony formation, biochemical reactions of various metals in the medium, dyes or sugars in the medium and enzymes in the microorganisms. Color readings and colony counts allow quantitative analysis of the presence and presence of specific microorganisms. For example, microbes E. coli are red, Streptoccoccus is cyan, Proteus is brown, E. coli O157 is soft purple, and Coliform is blue.
  • the valve attaches a thin film adhesive tape to the pores to close the pores during the distribution storage period, thereby completely closing the hydraulic pressure formed in the fluid by the centrifugal force of the disk.
  • a valve is preferable in which the thin film adhesive tape is peeled from the pores with a force to open the pores.
  • a valve for releasing the thin film adhesive tape from the pores and opening the pores by the force of hydraulic force formed in the fluid itself by the centrifugal force of the disk is referred to as a "hydraulic burst valve”.
  • This "hydraulic burst valve” is not only a valve in the form of a thin film, but also a thin film adhesive tape (flexible), it is well adapted to expansion and contraction according to environmental factors such as temperature, so that the liquid evaporation or There is an advantage that the sealing (sealing) problem due to the expansion and contraction of the body does not occur.
  • the closing strength is determined by the adhesive area of the thin film adhesive tape when the hole is closed by the thin film adhesive tape, and the thin film adhesive tape is torn apart at a disk rotation speed (centrifugal force) that overcomes the closing strength.
  • the pores open.
  • the thin film adhesive tape is weakened by the heat of the irradiated laser beam, and the opening of the valve is desired, the thin film adhesive tape is easily detached from the pore by applying the laser beam to the hole.
  • the valve is preferably a valve for attaching a thin film adhesive tape to close the pores during the distribution storage period to completely close the open pores and cutting the thin film adhesive tape by the heat of the laser to open the pores.
  • a valve that opens the pores by cutting the thin film adhesive tape by the heat of the laser is referred to as a "laser burst valve”.
  • the color of the thin film adhesive tape is preferably black, in order to absorb the laser beam well.
  • the valve seals the pores by the adhesive force or the binding force between the pores and the stopper to block the pores during the distribution storage period, and the stopper is separated from the pores by the centrifugal force by the high-speed rotation of the disc in use. It is an object of the present invention to provide a microbial analysis device using the open stopper valve and an analysis method using the same.
  • the stopper is preferably an adhesive material which melts by heat or is coated with an adhesive on the face of the hole for adhesion with the stopper and the hole.
  • a laser beam absorbing material it is also preferred to coat a laser beam absorbing material around the stopper to absorb the laser beam.
  • a laser beam absorbing material has a property of easily generating heat by absorbing a laser beam during laser beam irradiation.
  • the pressure sensitive adhesive is weakened or melted by the heat of the irradiated laser beam. Therefore, if you want to open the valve, apply a laser beam to the stopper is easily detached by the rotational force.
  • stopper burst valve a valve in which the stopper blocking the hole by the centrifugal force of the disk overcomes the adhesive or bonding force between the stopper and the hole and is separated from the hole so as to open the hole.
  • the stopper burst valve is referred to as a "stopple burst valve”.
  • bush burst valve is preferably a bead or thin film cylindrical stopper, but the shape is not particularly limited as long as it can perform the above-described functions or functions.
  • the valve seals the oil hole by the attraction force between the constrained groove made of a magnetic material and the magnetic valve, and the magnetic valve may be released from the constrained groove by the centrifugal force by the rotation of the disk to open the oil hole.
  • the magnetic valve which blocks the hole by the centrifugal force of the disk overcomes the attraction force with the restraint groove and is separated from the restraint groove to open the hole. It is called).
  • magnetic burst valve is preferably a bead, a thin film cylindrical magnet or a thin film rectangular magnet, but the shape is not particularly limited as long as it can perform the above-described functions or functions.
  • the magnet valve that has been removed from the restraint groove is closed again by the attraction force with the restraint groove.
  • the magnet valve is preferably demagnetized by the heat of the laser beam by irradiating the laser beam, so that the magnetic force is weakened. Therefore, if you want to open the valve and apply a laser beam to the magnet valve, the magnet valve is easily opened by the rotational force.
  • the invention is characterized in that the valve is made by a hydrophobic channel formed between the upper body and the lower body. Since the hydrophobic channel is hydrophobic, it is difficult for the fluid to move normally and the fluid can pass only through the centrifugal force generated when the body rotates.
  • the hydrophobic channel is formed between the upper body and the lower body when the upper body and the lower body are bonded by a thin film adhesive tape having a channel shape. That is, the upper body and the lower body are closely attached to each other by a thin film adhesive tape to form a single body, wherein a hydrophobic thin film channel is formed by a portion in which the thin film adhesive tape is missing between the upper body and the lower body, that is, the channel shape. .
  • the height of the hydrophobic thin film channel is determined by the thickness of the thin film adhesive tape.
  • a valve that overcomes the resistance to hydrophobicity preventing the fluid from moving by the centrifugal force of the body and moves the hydrophobic thin film channel is referred to as a "hydrophobic burst valve”.
  • burst valve the "hydraulic burst valve”, the “bump burst valve”, the “magnetic burst valve, the” laser burst valve "and the” hydrophobic burst valve "will be collectively referred to as” burst valve ".
  • the burst valve is opened by heat generated by the laser beam and centrifugal force or centrifugal force.
  • the laser beam from the laser beam generator is irradiated around the burst valve to weaken the sealing force or coupling force of the burst valve by heat by the laser beam so that it can be easily opened by centrifugal force.
  • the laser beam generator is preferably mounted on a slider.
  • the first method is to move the laser beam generator by moving the laser beam to a radial position of the burst valve in order to irradiate the laser beam to the burst valve during disk rotation.
  • a laser beam is irradiated to the burst valve, in which case the amount of laser beam irradiated to the burst valve is the rotational speed of the disc and the laser
  • the laser beam irradiation operation during the rotation of the disk which is a function of the power of the beam generator, is hereinafter referred to as " scanning beam " operation in the present invention.
  • the laser beam generator is moved by moving the laser beam to the burst valve at the radial position of the burst valve during disk rotation, and the laser beam generator and the corresponding burst valve are rotated during the disk rotation.
  • the laser beam is turned on every time (ie, encountering) and off otherwise.
  • This laser beam irradiation operation is hereinafter referred to as "pulse beam" operation in the present invention.
  • the opening of the burst valve is characterized by disk rotation after heating of the burst valve by the "pulse beam” operation or the “scanning beam” operation.
  • the opening of the burst valve on the concentric circle of the microbial analyzer is performed by the centrifugal force generated in the fluid during the rotation of the microbial analyzer and the laser beam generator installed on the slider during the rotation of the microbial analyzer and the burst on the concentric circle.
  • a "pulse beam” or “scanning beam” operation in which the burst valve is heated to open each time it matches a valve, such a “pulse beam” or “scanning beam” operation causes the high viscosity fluid to move.
  • Fluid with high viscosity is easy to move with centrifugal force. Highly viscous fluids may not move to neighboring chambers even when the valve is open during shutdown, which may cause reproducibility and reliability problems.
  • the disk preferably has at least one medium chamber arranged concentrically.
  • the image sensor for photographing the medium chamber of the disk; And a spindle motor for rotating the disk.
  • the entire shooting of the concentric discharge chamber may be performed by an image sensor while rotating the spindle motor 360 degrees slowly, or by repeating the rotation and stopping of the spindle motor repeatedly to obtain an image at each stop and combine them. Get a 360 degree full image.
  • the image sensor is characterized in that the microbial colonies (colony) in the medium chamber by color classification and counting and quantitative analysis.
  • the count is a measure of the number of bacteria forming colonies in the medium chamber, and plate count or colony count is preferred.
  • the coefficients are expressed in bacterial counts or colony-forming units (cfu / ml or cfu / g).
  • the image sensor is characterized in that reading a barcode (barcode) on the disk.
  • the image sensor reads a bar code on the disc to authenticate the authenticity of the disc, or the data of the bar code and the user's information (for example, IP address, location, telephone number, e_mail address, company name, or The serial number of the disk, etc.) is remotely transmitted through the Internet to receive notification of authenticity of the disk and product identification information from the server.
  • the user's information for example, IP address, location, telephone number, e_mail address, company name, or The serial number of the disk, etc.
  • the microorganism analyzing apparatus may be connected to a computer or an internet network by the input / output device.
  • the microbial analyzer cannot drive the disc.
  • the disc is genuine, the barcode is registered in the central server, and when the product identification information is received from the central server, the microbial analysis apparatus drives the disc according to the driving software corresponding to the product identification information. If the driving software corresponding to the product identification information is not installed in the computer or the microbial analysis apparatus, the driving software is downloaded from the central server through the Internet network.
  • the storage device or the RF IC stores the usage history and the analysis result of the disk.
  • the central control unit is characterized in that the information stored in the storage device or RF IC to read and transmit to the server remotely through the input and output device.
  • a warning message is sent to the user, whether to be automatically ejected when loading a disc which is not usable in a conventional optical disc (for example, CD, DVD) or an unrecognized microbial analysis device.
  • a conventional optical disc for example, CD, DVD
  • an unrecognized microbial analysis device for example, CD, DVD
  • the present invention (a) injecting a sample to the prep chamber using a sampler; (b) transferring the sample in the preparation chamber to the media chamber; (c) applying and inoculating the medium surface in the medium chamber with the sample transferred to the medium chamber; (e) It provides an analysis method using a microbial analysis apparatus according to the present invention comprising a washing step of collecting the surplus specimen in the trech chamber after application inoculation.
  • the analysis method may further comprise a enrichment culture step.
  • the analysis method may further include reading the medium chamber by the image sensor.
  • the analysis method may further comprise a medium chamber reading step and the microbial colonization step.
  • the analysis method may further include transmitting the medium chamber reading result to a remote server.
  • the present invention provides a sample injection port for injecting a sample;
  • a sampler for collecting a sample from an inspection site and injecting the collected sample into the sample inlet;
  • a prep chamber for temporarily storing a sample injected from the sample inlet;
  • An enrichment chamber containing a sample from the prep chamber and containing a liquid culture medium for enriching the microorganisms present in the sample;
  • At least one medium chamber providing a culture medium for providing nutrients to the enriched microorganisms;
  • a trech chamber for collecting debris and excess sample;
  • a valve for moving fluid and sample between the chambers;
  • a flow path (channel) for providing a movement path of the specimen;
  • it provides a microorganism analysis device having a rotatable disk-shaped body in which the flow path, valve and chamber are integrated and an analysis method using the same.
  • the body may be selected from various materials such as plastic, glass, mica, silica, silicon wafer, and the like.
  • plastics are preferred for economic reasons, ease of processing, and compatibility with existing laser reflection based detectors such as CD-ROM and DVD readers.
  • Plastics that can be used include polypropylene, polyacrylates, polyvinyl alcohol, polyethylene, polymethyl methacrylate (PMMA), Cyclic Olefin Copolymer (COC), acrylics and polycarbonates. Of these, polypropylene, COC, acrylic and polycarbonate are preferred, and acrylic, COC and polycarbonate are most preferred.
  • the surface of the body may also be aluminum coated to prevent evaporation of the liquid stored in the chamber.
  • the prep chamber, enrichment chamber, medium chamber, trech chamber; And a flow path, a hole, and a valve for fluid movement between the chambers are formed in the disc-shaped body to constitute various processes required for microbial analysis, and the disc-shaped body is formed by stacking an upper body and a lower body. Characterized in that configured.
  • the cover means is closed by a magnetic force forming an attractive force between the upper body and the lower body, and the experimenter collects a sample of the suspected colony from the medium chamber through the opening of the cover means for identification experiments. It is done.
  • the cover means is preferably a permanent magnet is installed in the upper body and a ferromagnetic material is installed in the lower body is closed by the attraction between the upper body and the lower body.
  • cover means further comprises an opening means, allowing easy opening by the opening means.
  • the opening means is preferably an electromagnet board in which an electromagnet is generated which generates a repulsive force on the permanent magnet in the upper body.
  • an electromagnet board on (ON) When the electromagnet board on (ON) generates a repulsive force for the permanent magnet in the lower body, it is possible to easily separate the lower body from the body.
  • the upper body and the lower body may be combined by ultrasonic welding or laser welding, a thin film adhesive tape. Since such lasers generate heat well at the interface between materials having different light transmittances, it is preferable that the upper body and the lower body use different kinds of plastic materials for laser welding.
  • the thin adhesive tape is preferably an adhesive (a gluing agent) used for all adhesive tapes such as double-sided tape, and the adhesive is silicone, rubber, modified silicone, acrylic, Materials such as polyester, epoxy and the like can be used.
  • the double-sided tape is surface treated with an adhesive (a adhesive) on both or one side of the release paper such as paper, vinyl, polyester film, polyethylene film and other synthetic materials.
  • an adhesive a adhesive
  • the release paper such as paper, vinyl, polyester film, polyethylene film and other synthetic materials.
  • a pressure-sensitive adhesive material having characteristics such as high sealing and buffering, vibration relaxation, impact resistance, heat resistance, adsorption, and adhesive strength.
  • a thin film coating by adhesive on one side of the body or by applying a dispenser or spray or silk screen printing on the adhesive It is preferable to apply a thin film coating on one side of the adhesive.
  • the body of one microorganism analyzing apparatus is assembled by attaching a thin film-coated upper body and a lower body to each other in close contact with each other.
  • a pore closure membrane of the double-sided tape "hydraulic burst valve” is included, so that it is preferred to leave a pore-closing membrane with an adhesive on the pore area when removing the release paper or to form a pore-closing membrane for the "hydraulic burst valve" during the thin film coating.
  • the porous closed membrane may be a membrane that can be easily torn by hydraulic or centrifugal force instead of a thin film adhesive tape, and the surface of the membrane is hydrophobic without affinity with water.
  • Polymer membranes such as polyethylene (PE), polypropylene (PP), polysulfone (PS), polyalkene, cellulosics, polyvinyl, polycarbonate, and polyamide may be used as the membrane.
  • the hydrophobic membrane surface effectively blocks the movement of fluid stored in the chamber.
  • the stopper is characterized in that the cylinder, or a ball (ball) or a thin film selected from the shape of the cylinder.
  • the stopper is a pressure-sensitive adhesive coated wax or a wax is preferred.
  • the wax is preferably paraffin wax, synthetic wax, or microcrystalline wax.
  • the adhesive or the adhesive coated on the surface of the plug is preferably melted or weakened by the heat of the irradiated laser beam.
  • the adhesive has a weak adhesive strength of the burst valve due to the heat of the irradiated laser beam, and the burst valve is opened by the disk rotation.
  • Microbial analysis device and an analysis method using the same of the present invention is suitable for detecting a small amount of microorganisms in the fluid.
  • the microbial analysis apparatus according to the present invention and an analysis method using the same provide an effect of automating a series of processes such as microbial enrichment, application (inoculation), culture, and detection.
  • 1 to 4 is a view showing various embodiments of a burst valve used in the microbial analysis apparatus according to an embodiment of the present invention
  • 5 to 7 illustrate various embodiments of a “magnetic burst valve” using a cross sectional view of the “magnetic burst valve” and a magnet valve embedded in a disc;
  • FIG. 8 to 10 is a view schematically showing a microbial analysis apparatus according to an embodiment of the present invention.
  • FIG. 11 is another embodiment of a turntable and a crimping means
  • FIG. 14 is a view showing a sampler used in the microbial analysis apparatus according to an embodiment of the present invention.
  • 15 is a view showing the results of culturing microorganisms in the medium chamber
  • FIG. 16 is a diagram illustrating an embodiment of a top loading microorganism analysis device incorporating a thermostat or a temperature control device.
  • FIG. 17 is a view showing an embodiment of a front loading type microbial analysis apparatus in which a thermostat or a temperature control device is built into a tray.
  • FIG. 18 is a view showing an embodiment in which the cover means is installed on the outermost and innermost circumference of the body
  • 19 is a view showing an embodiment of an opening means using an electromagnet board
  • FIG. 20 is a view showing an embodiment of an oxygen supply valve for supplying oxygen to the aerobic microorganisms in the medium chamber
  • FIG. 21 is a diagram illustrating an embodiment of a microbial analysis apparatus using a test strip.
  • FIG. 1 to 4 illustrate various embodiments of a burst valve used in a microbial analysis apparatus according to an embodiment of the present invention.
  • FIG. 1 and 2 are cross-sectional views and exploded views of a-b of the hydraulic burst valve using a thin film adhesive tape installed in the disk or body of the microbial analyzer.
  • FIG. 2 shows that the hole closing film 13a is torn off by the hydraulic pressure generated in the liquid (not shown) by the rotation of the body 100 so that the hole 10b is opened in the chamber 11a. It indicates that the stored liquid has been moved to the chamber 11b.
  • the body 100 includes an upper body 1 and a lower body 2, each of which includes a flow path 16a through which fluid can flow on the body surface during the injection molding process; Chambers 11a and 11b capable of storing a sample or a solution such as a liquid culture solution; And a hole 10b connecting the chambers 11a and 11b.
  • the upper and lower bodies 1 and 2 are closely attached to each other by the thin film adhesive tape 2a to form one body 100.
  • the upper body 1 and the lower body 2 constitute the chambers 11a and 11b
  • the lower body 2 has a flow path 16a for connecting the chamber 11a and the chamber 11b. Is engraved to a certain depth, and a hole 10b is formed at the distal end of the flow path 16a to provide a connection between the chamber 11a and the chamber 11b. This hole 10b is closed by the hole blocking membrane 13a.
  • the hole closing membrane 13a is formed at the portion of the hole 10b by the thin film adhesive tape 2a when the upper body 1 and the lower body 2 are attached and assembled.
  • Closing the pores 10b by the hole closing membrane 13a completely blocks the pores 10b during the distribution storage period, and the fluid itself stored in the chamber 11a by centrifugal force by the high speed rotation of the body 100 during use.
  • the hole closed membrane 13a is torn off by the hydraulic pressure formed in the hole, and the hole 10b is opened to move the fluid into the chamber 11b. Since the pore-closing membrane 13b is flexible, it adapts well to expansion and contraction according to environmental factors such as temperature, and thus sealing problems due to evaporation of liquid or expansion and contraction of the body during distribution. There is an advantage that does not occur.
  • the pore-closing membrane 13b is formed in the hole 10b when the upper body 1 and the lower body 2 are attached and assembled in close contact with each other by a thin film adhesive tape 2a. It features.
  • the closing strength when closing the pores by the thin film adhesive tape 2a is proportional to the adhesion area 14 between the pore-closing membrane 13a and the lower body 2, so that the body
  • a plurality of "hydraulic burst valves" are installed in the 100 and their closing strengths are different from each other so as to open a desired valve at a desired point in time so as to provide a hydraulic pressure greater than the closing strength of the closed hole membrane 13b of the corresponding valve.
  • Hydraulic force, hydraulic pressure) to generate a centrifugal force is characterized in that the hole (10b) to be opened selectively or individually.
  • the adhesive strength of the "hydraulic burst valve” is weakened by applying heat to the hole-closing membrane 13b using a laser beam, and the hydraulic burst valve is easily opened by disk rotation.
  • the pore-closing membrane 13a is characterized by constituting a thin film adhesive tape layer by a phase change material.
  • the phase change material preferably has a melting point of 40 degrees to 70 degrees.
  • the pore-closing membrane 13a is made of a thermoplastic adhesive material, and the remaining bonding portion constitutes a thin film adhesive tape layer by a thermosetting adhesive material.
  • the pore-closing film 13a may be a thin film adhesive tape formed by a thermoplastic hot malt adhesive, and the remaining joint may be a thin film adhesive tape formed by an acrylic adhesive which is thermosetting.
  • the adhesive force of the pore-closing membrane is weakened more easily by the heat of the laser beam than in the remaining portions.
  • the pore-closing film 13a is easily softened by the heat of the ray point beam, and thus weakens the adhesive force, while the surrounding and the remaining parts are bonded by the heat-curable tape, thereby providing the advantage that the adhesive force is not weakened by the heat of the laser beam. .
  • the softening temperature of the thermosetting pressure-sensitive adhesive (Softening Temperature) is preferably 120 degrees or more, and the thermoplastic pressure-sensitive adhesive The softening point is more preferably between 60 and 80 degrees.
  • thermoplastic resin is preferably COC, PMMA, PC, PS, POM, PFA, PVC, PP, PET, PEEK, PA, PSU and PVDF.
  • 3 and 4 show an example of a “bung burst valve” using a plug embedded in the body 100.
  • the body 100 is composed of an upper body (1) and the lower body (2), each of which has a flow path (16a) through which fluid can flow on the body surface during the injection molding process; Chambers 11a and 11b capable of storing solutions; And the hole (10b) for connecting the chamber (11a, 11b) is formed.
  • the hole (10b) is closed by a stopper (13b) buried in the hole, by closing the hole by the stopper (13b) to completely block the hole during the distribution storage period, by the high-speed rotation of the body 100 in use
  • the stopper 13b is released toward the auxiliary channel 16b so that the hole 10b is opened.
  • another aspect of the present invention is because the closing strength (closing strength) when closing the hole (10b) by the stopper (13b) is proportional to the contact area between the stopper and the flow path (16a), a plurality of "stopper" in the body 100 Burst valves "and their closing strengths are different from each other so that the perforations 10b can be selectively or independently by rotating the disc to generate a centrifugal force above the closing strength of that valve to open the desired valve at a desired point in time. It is characterized in that the opening.
  • the diameter of the stopper is preferably 1mm to 5mm. As the diameter increases, the contact surface increases, thereby increasing the reliability of opening and closing.
  • the plug may be spherical but thin film particles may also be used. In the present invention, a thin film cylinder or a thin film square is preferred as the thin film particles. The thickness of the thin film particles is preferably about 0.1 mm to 2 mm.
  • FIG. 4 shows a case in which the oil hole 10b is blocked by the stopper 13b and the flow path 16a is blocked.
  • the oil hole 10b is generated by the rotation of the body 100.
  • 5-7 are various embodiments of a "magnetic burst valve” using a cross sectional view between a-b of the "magnetic burst valve” and a magnet valve embedded in the body 100.
  • Reference numeral 90 denotes a magnet valve composed of permanent magnets, and the magnet valve closes the oil hole 10b by the attraction groove and the attraction force 101 formed of the magnetic material.
  • the magnetic valve 90 is separated from the restraint groove 101 by the centrifugal force to open the oil hole 10b.
  • the boundary membrane 91 is installed so that the detached magnet valve 90 does not deviate more than a certain range.
  • the restraint groove 101 serves to prevent the magnetic valve 90 from falling off by shaking the body 100.
  • the diameter of the constrained groove 101 is preferably 20% to 70% larger than the diameter of the magnet valve 90.
  • the magnetic valve 90 may be formed of a permanent magnet and may be coated with a rubber cushion material such as silicone rubber thereon to increase the sealing force of the pores. Thin film circular magnets, thin film cylindrical magnets or thin film square magnets or ball magnets are preferred.
  • FIG. 6 shows the case where the hole 10b is opened by centrifugal force, and the figure below shows the case where the hole 10b is closed.
  • the body (1, 2) has the advantage that it does not have to be a ferromagnetic material.
  • FIG. 8 to 10 illustrate an embodiment of a disk 100 and peripheral drive devices in which various processes for microbial analysis are integrated as an embodiment of the microbial analysis apparatus 200 of the present invention. .
  • the disk 100 is composed of the upper body 1 and the lower body 2, and is adhered to each other by a thin film adhesive tape (2a) to form a single disk (100).
  • Reference numeral 120 denotes a sample injection means selected from a dispenser, pipette, dice, sampler, and lancet for injecting a sample
  • reference numeral 121 denotes a sample injection port for injecting a sample
  • Reference numeral 130 is a preparation chamber for temporarily storing a sample injected by the sample injection means 120 and performing a preparation process
  • reference numeral 132 denotes a culture medium for a culture process for providing nutrients to the microorganisms in the sample.
  • Is a media chamber Is a media chamber
  • a reference numeral 133 denotes a trash chamber for a cleaning process for collecting the residue and excess sample
  • reference numerals 70 and 71 denote a valve for moving the fluid and the sample between the chambers.
  • reference numeral 170 denotes a disk gap.
  • Reference numeral 335 denotes a reference hole indicating a reference of the body.
  • reference numeral 91 is a bar code that may include information about the product ID, expiration date, the type of microorganism that can be analyzed and diagnosed, etc. of the disc.
  • the image sensor 103 not only reads the barcode 91 on the disc, but also photographs the medium chamber 132 to quantitatively or qualitatively analyze the microorganism culture result in the medium chamber 132. Get a color image of
  • the preparation chamber 130 may further include an enrichment chamber for containing or storing a liquid culture medium for enriching the microorganisms present in the sample.
  • Reference numeral 211 denotes a slider mounted on the laser beam generator 5a and connected to a slide motor 109 to be driven and controlled to generate a laser beam for the burst valve by movement of the slider. Radial space addressing of the device 5a is achieved.
  • the radial space addressing is by means of a slide motor 109 capable of reversibly moving the slider in the radial direction.
  • the rotation of the slider motor allows the slider to move radially outward from the center of the body or from the outside of the body to the center of the body.
  • the opening and closing control of the valve at the start point and the end point of each of the above processes is performed by a burst valve.
  • the body 100 is rotated while the laser beam generator 5a installed on the slider 211 is spatially moved to the radius of the valve and the laser beam is turned on. Heating the burst valve opens the valve by centrifugal force during rotation. At this time, the laser beam generator 5a operates in the "pulse beam” or "scanning beam” mode.
  • Reference numeral 110b is a flexible cable for connecting various control signals required for the laser beam generator 5a and the image sensor 103 on the slider 211 to a wafer or a harness. 110a is connected to the central control unit 105.
  • Reference numeral 181 denotes a turn table for placing the disk 100, which is front or top loaded on the turn table through the central void 170.
  • Reference numeral 188 is a memory-embedded wireless RF IC or electronic tag device that includes protocols for analyzing microorganisms, analysis algorithms, and standard control values for reading.
  • personal encryption information and identification (identification) of the microbial analysis device can be stored, so that others can not be used without permission.
  • the wireless RF IC 188 is preferably in the form of a smart IC card.
  • the wireless RF IC 188 information is provided to the central control unit 105 through wireless transmission and reception, and is utilized for personal encryption.
  • Reference numeral 110 is a radio wave generation unit for supplying power to the wireless RF IC 188.
  • the radio wave generated by the radio wave generator generates a sufficient amount of electricity by supplying a power to the radio RF IC 188 by sensitizing the induction coil coil embedded in the radio RF IC 188 according to Fleming's law.
  • the wireless RF IC 188 has a temperature measuring function, and measures the temperature of the medium chamber to wirelessly transmit to the central control unit 105.
  • the central control unit 105 maintains a constant temperature by the heating means 240 or the cooling means.
  • the temperature of the medium chamber 132 is preferably maintained at a temperature selected between 35 degrees Celsius and 40 degrees Celsius suitable for microbial growth.
  • the cooling means is preferably by rotation or rotating fan of the body 100.
  • the disk is rotated or the fan is rotated to cool the heated medium chamber 132 by wind while cooling the medium chamber 132 to a desired temperature based on the temperature measured by the RF IC. It is preferred to rotate the disk 100 further to make it work.
  • the wireless RF IC 188 is characterized in that the temperature of the discharge chamber 132 is read and wirelessly transmits the result to the central control unit 105.
  • the heating means is preferably a nano pattern 240 connected to an output terminal of the RF IC, and the heating means 240 is controlled by on / off intervals and power amounts of power supply of the RF IC. It is preferred that the temperature of is controlled.
  • heating means is made by a scanning beam operation of the laser beam generator 5a.
  • the wireless RF IC 188 includes information on an inspection date and a test result, an expiration date of the test, and a microbial analysis result according to the test of the microbial analysis device. It is done. After the microbial analysis, the information on the microbial analysis device may be brought to the RF IC reader or the body 100 may be loaded into the microbial analysis device.
  • the microorganism analysis result is preferably a color image of the medium chamber 132 obtained by the image sensor 103.
  • the test results analyze the color image of the medium chamber 132, the colony density and population of the colony (population) according to the type of microorganism is preferred.
  • the microorganism analyzing apparatus of the present invention preferably includes a microscopic sensor 106 for observing microorganisms and a central controller 105 for processing an image from the microscopic sensor and counting colonies or checking whether cells are dead. do.
  • the central control unit 105 stores the test result, the test date, and the microbial analysis result of the disk 100 in the memory or storage device 113 embedded in the wireless RF IC 188. It is characterized by storing in).
  • the input / output device has a communication standard of USB (Universal Serial Bus) or IEEE 1394 or ATAPI or SCSI or Internet communication network.
  • USB Universal Serial Bus
  • IEEE 1394 Universal Serial Bus
  • ATAPI ATAPI
  • SCSI Internet communication network
  • information about the sample itself such as the name of the sample, the collection place of the sample, the collection date and time of the sample can be input.
  • FIG. 9 shows an embodiment of the upper view of a slider 211 in which the laser beam generator 5a and the image sensor 103 are installed.
  • the slider is movement controlled by worm gear connections 109a and 109b connected to the axis of the slide motor 109.
  • the slider is slidably moved using the slide arms 108a and 108b as guides.
  • the slide arms 108a and 108b are fastened to the body of the microbial analysis apparatus 200 through screws 110a to 110d.
  • Reference numeral 110b denotes a flexible cable and is connected through a wafer or harness 110a.
  • Reference numeral 181 denotes a turn table that is rotated by the spindle motor 102 described above.
  • reference numeral 200a denotes an outer body supporting the microorganism analyzing apparatus 200.
  • a circuit board 140 is jointly fastened to the outer body 200a at the bottom of the microorganism analyzing apparatus 200, and a central controller 105 and a storage device for controlling the microorganism analyzing apparatus 200 on the circuit board.
  • 113 and an input / output device 111 are designed to be disposed on the circuit board 140.
  • the central controller 105 not only controls the spindle motor 102 for rotation or stop of the disk 100, but 2) design arrangement on the slider 211 by the control of the slide motor 109.
  • 3) serves to move the position of the laser beam generator 5a to the radial position of the valve in order to apply heat to the valve during the valve opening and closing operation.
  • the image sensor 103 may be mounted on the slider 211 or disposed on the circuit board 140. Note that only the image sensor 103 is mounted on the slider 211.
  • the central control unit 105 determines whether the disk currently loaded in the microbial analysis apparatus 200 is for microbial analysis.
  • the unique ID of the disk 100 to the central control unit 105 via the wireless RF IC 188 at the time when the disk 100 is loaded into the microbial analysis apparatus 200.
  • the central control unit 105 recognizes whether the disk 100 currently loaded in the microbial analysis apparatus 200 is a disk for microbial analysis.
  • Another aspect of the present invention by sensing the barcode on the disk by the image sensor 103 at the time when the disk 100 is loaded into the microbial analysis device and by analyzing it by the central control device 105, The central controller 105 recognizes that the disk loaded in the analysis device 200 is a disk for analyzing microorganisms.
  • a specific mark or pattern on the disk is sensed by the image sensor 103 at the time when the disk 100 is loaded into the microbial analysis apparatus and the central control device ( By analyzing by 105, it is characterized in that the central control unit 105 recognizes whether the disk currently loaded in the microbial analysis apparatus 200 is a genuine microbial analysis disk.
  • the image information about the discharge chamber 132 obtained by the image sensor 103 may be transferred to the central control unit 105, the storage unit 113, or the input / output unit through the flexible cable 110b connected to the slider 211. 111).
  • the image information of the discharge chamber 132 obtained by the image sensor 103 on the slider 211 or the image sensor arranged and designed on the circuit board 140 may be transmitted to the central control unit 105, the storage unit 113, or the input / output unit. Is sent to the device 111.
  • Reference numeral 104 is a crimping means of the disk 100 loaded in the disk cavity 170 is preferably pressed by the magnetic force with the ferromagnetic material (104a) is preferably designed to enable vertical movement and idle rotation.
  • the ferromagnetic material (181a) is preferably compressed by the magnetic force with the turntable (181).
  • the turntable 181 and the crimping means 104 are preferably permanent magnets.
  • the slider 211 may further mount a movable permanent magnet 5b.
  • the image sensor 103 performs a "search process of the discharge chamber" before capturing an image for the discharge chamber 132.
  • the permanent magnet 5c is provided on the body 100 to facilitate optical alignment between the image sensor 103 and the medium chamber 132. It is characterized by.
  • the short rotation is preferably a rotation of 0.1 second to 0.5 seconds.
  • FIG 11 is yet another embodiment of the turntable 181, the ferromagnetic material (181a) and the pressing means 104.
  • the ferromagnetic material 181a has a groove 181b to be mechanically engaged with knobs 181c and knobs of the turntable 181.
  • Reference numeral 40 is at least one light emitting diode (LED) for illumination of the image sensor, the image sensor 103 or the LED is mounted on the slider 211 or the upper side of the discharge chamber 132 Or it can be installed at the bottom.
  • LED light emitting diode
  • the image sensor 103 is preferably a linear image sensor that senses the amount of light in a CCD or CMOS or pixel unit.
  • the linear image sensor is preferably a linear sensor array or a contact image sensor (CIS).
  • the image sensor 103 is characterized in that to move the slider 211 to the radius corresponding to the discharge chamber 132 to obtain the image information of the discharge chamber 132.
  • the disk 100 is an embodiment in which the upper body 1 and the lower body 2 are laminated and bonded by an adhesive tape 2a, and the hydraulic burst valve is employed as a valve.
  • Reference numerals 121a, 121b, 121c, and 121d indicate sample inlets for injecting a sample, and the disk 100 includes at least one prep chamber 130a to 130d for temporarily storing a sample injected through the sample inlet. ; At least one medium chamber (132a, 132b, 132c, 132d) providing a medium (80) for providing nutrients to the microorganisms in the sample; Trash chambers 133a, 133b, 133c, and 133d for collecting debris and excess samples; And valves 70a, 70b, 70c, 70d, 71a, 71b, 71c and 71d for moving the fluid and the sample between the chambers.
  • Reference numeral 170 denotes a disk gap.
  • Reference numeral 335 denotes a reference hole indicating a reference of the body.
  • Each prep chamber 130a, 130b, 130c, 130d may be injected with different or the same kind of sample.
  • the medium chambers 132a, 132b, 132c, and 132d may contain the same medium or different types of medium. Therefore, with the disk 100, it is possible to test for multiple microorganisms on the single specimen and to test a single microorganism on the multiple specimens.
  • the hydraulic burst valves 70a to 70d provided therein must be opened, respectively.
  • the internal pressures to open the hydraulic pressure valves 70a to 70d installed on the inside first.
  • the adhesion area of the thin-film adhesive tape of the burst valves 70a to 70d is larger than the hydraulic burst valves 71a to 71d provided on the outside to increase the closing strength, or the hydraulic burst valves 70a to 70d provided on the inside.
  • the hydraulic burst valves 70a to 70d installed inside by the laser beam generator 5a should be heated by a "pulse beam” or "scanning beam” operation.
  • Samples moved into the medium chamber 132 by opening the hydraulic burst valves 70a to 70d should be evenly applied on the surface of the medium 80 so that the inoculum is inoculated onto the medium.
  • the inner height 131a of the discharge chambers 132a to 132d is smaller in height than the outer height 131b. Therefore, the sample introduced into the medium chamber is collected in the direction of the center of the medium chamber by the capillary phenomenon. Subsequently, when the disk is rotated, the sample is moved outward in the radial direction of the medium chamber by centrifugal force. Then, when the disk 100 is stopped rotating, the sample is moved back inside the medium chamber by capillary action. If this is repeated several times, the sample is evenly applied to the surface of the medium (80). Thereafter, the burst valves 71a to 71d are opened to move the excess sample to the trech chambers 133a to 133d.
  • the prep chambers 130a to 130d are preferably hydrophilic coated, and the hydraulic burst valves 70a to 70d installed therein are the "magnetic burst valves", “hydrophobic burst valves” and “laser burst valves” described above. It may be replaced by any one or more of ".
  • the chamber height of the preparation chambers 130a to 130d it is preferable to design the chamber height of the preparation chambers 130a to 130d to be smaller than the chamber height of the medium chambers 132a to 132d. Due to this configuration, it is possible to prevent the sample from flowing into the medium chamber by capillary phenomenon in the preparation chamber itself during sample injection.
  • the trash chambers 133a to 133d are preferably hydrophilic coated, and the hydraulic burst valves 71a to 71d installed on the outside are the "magnetic burst valve", “hydrophobic burst valve” and “laser burst” described above. It can be noted that any one or more of “valve” may be replaced.
  • the chamber height of the thresh chambers 133a to 133d it is preferable to design the chamber height of the thresh chambers 133a to 133d to be smaller than the chamber height of the discharge chambers 132a to 132d. Due to this configuration, the debris and excess sample once moved to the tresh chamber can be prevented from flowing back to the medium chamber by capillary action in the trech chamber itself. 13 is a detailed view of the hydraulic burst valve 71b.
  • the inoculation of the medium 80 by applying the surface of the medium 80 with a sample and moving the excess sample to the tresh chamber is referred to as "coating inoculation".
  • coating inoculation By the application inoculation, it is possible to automate the streaking operation that has been done by conventional manual work.
  • the sampling rod 300a is separated from the sampler 300 (step 1).
  • Reference numerals 63 and 63a provide a screw fastening portion to be detachable between the sampling rod 300a and the culture tube 300b.
  • a cotton swab (60) for collecting the specimen from the inspection site. The user collects a sample using a swab 60 from a suspected place or inspection site 55.
  • the sampling rod (300a) and the culture tube (300b) is coupled through a screw fastening portion (63, 63a) and incubated for 24 hours enrichment (step 2).
  • the culture tube (300b) is enriched culture medium 69 is stored to help the growth of microorganisms.
  • the enrichment broth is preferably peptone water.
  • the culture cap 67 is separated from the sampler 300 (step 3).
  • Reference numerals 67 and 67a provide a screw fastening portion to be detachable between the culture cap 67 and the culture tube 300b.
  • the enriched sample in the culture tube 300b is transferred through the sample inlet 121 of the disc 100 (step 4).
  • the enriched sample in the culture tube 300b is transferred to the preparation chambers 130a to 130d.
  • Reference numeral 69 serves to filter the foreign matter in the enrichment culture medium 69 to prevent the transfer to the disk 100 by a filter.
  • the specimen stored in the preparation chambers 130a to 130d is moved into the medium chambers 132a to 132d by opening the burst valves 70a to 70d.
  • the surface of the medium in the medium chamber is coated with a sample by the coating inoculation operation.
  • burst valves 71a to 71d are opened to transfer the surplus specimens in the medium chamber to the trech chambers 133a to 133d.
  • microorganisms are cultured in a media chamber for 24 hours.
  • the medium chamber is read by the image sensor, and the colony is counted by the central controller to qualitatively and quantitatively analyze the microorganism.
  • test result according to the reading result is optionally displayed on the computer monitor, and remotely connected and remotely transmitted via the internet network automatically or manually.
  • the remote site calculates the user's hygiene score based on the inspection result and retransmits the user's hygiene score to the input / output device.
  • the hygiene score is preferably calculated based on the number of tests and the colony density of microorganisms.
  • 15 is an example showing the result of culturing microorganisms in the medium chamber.
  • Each spot represents a colony of microorganisms and different colors for each microorganism when chromogenic media is used.
  • the microbial analysis apparatus 100 determines whether the user ejects or stops the disk. If the user ejects or stops the disk, the microbial analysis apparatus continues the analysis while ignoring it. At this time, a warning message is notified to the user or a password is required. If the password is correct, the user will be asked to eject or stop the disk.
  • the memory of the wireless RF IC 188 stores a disk usage history, expiration date information or information on the types of microorganisms that can be analyzed.
  • the barcode pattern stores the identification information or the expiration date information of the disk or the type of microorganism to be analyzed.
  • the expiration date information informs the user that the expiration date is also unavailable for the disc.
  • FIG. 16 is a toploading microorganism analysis incorporating a thermostat or a temperature control device 730 for maintaining an optimal constant temperature during enrichment of microorganisms in the sampler 300 in step 2 of FIG.
  • a thermostat or a temperature control device 730 for maintaining an optimal constant temperature during enrichment of microorganisms in the sampler 300 in step 2 of FIG.
  • One embodiment of the apparatus 200 is shown.
  • the enrichment culture is preferably made by inserting the culture tube 300b of the sampler 300 into a thermostat or a temperature control device 730.
  • the microorganism analysis apparatus 200 of the disk may be loaded by opening the cover 751 for top loading and fitting the disk 100 to the turntable 181.
  • FIG. 17 is a view showing an embodiment of a front loading type microorganism analyzing apparatus incorporating a thermostat or a temperature control device, and the temperature sensor 731 to maintain an optimal constant temperature during enrichment of microorganisms.
  • the enrichment culture is preferably made by front loading by inserting the void 171 of the body 100 to the turntable 181 in a thermostat or temperature control device 730.
  • Reference numeral 733 is a transparent window, which allows to observe the culture state from the outside during the culture period.
  • the microbial analysis apparatus 200 may include an analysis start button 745 and a stop button 746.
  • reference numeral 742 denotes a power on / off button of the microbial analysis apparatus
  • reference numeral 741 denotes an LED for indicating a power state.
  • Reference numeral 760 denotes a display device for displaying a progress state of the microorganism analyzing apparatus 200, and a liquid crystal display (LCD) is preferred as the display device.
  • LCD liquid crystal display
  • the display device 760 displays an analysis result or displays a progress state according to a main process of the microbial analysis device.
  • the display device 760 may display the progress according to the main process (prep process, enrichment process, culture process) and steps in the form of a percentage (%) or a bar graph.
  • the display device 760 may provide a graphic user interface.
  • the graphical user interface preferably allows the user to provide temperature settings, incubation time settings for the thermostat. It is also preferred to notify the user via an alarm when the incubation time has elapsed.
  • the temperature control device applies a carbon or carbon nanotube, which is a high resistance material, onto a heater 761 in the tray 761 by applying a carbon or carbon nanotube, which is a high resistance material, on a heater or a PET (Polyethylene Terephthalate) film. It is preferred to include.
  • the microbial analysis device is further provided with calculation software for quantifying negative, positive or microbial levels for a specific microorganism by reading and analyzing an image of the medium chamber.
  • the cover means is installed at the outermost and innermost circumferences of the body 100. It is preferred that the cover means is provided with a permanent magnet 820b in the lower body 2 and a ferromagnetic material 820a in the upper body 1 to be closed by the force between the upper body and the lower body.
  • the solid medium in the medium chambers 132a to 132d contains a large amount of water. Unlike in FIG. 16, if the culture is carried out straight with the medium installed in the lower body 2, evaporation occurs, resulting in moisture and water droplets on the ceiling of the upper body 1, and the drop of water falls on the medium, thereby destroying the culture state. Not only is this possible, the medium can quickly dry out by evaporation during cultivation.
  • the medium 80 is installed on the upper body 1 side, as shown in FIG.
  • the sample inlet (121a to 121d) is installed on the upper body (1) side.
  • the medium in the medium chamber is preferably a solid medium to an agar medium.
  • Reference numeral 335 denotes a reference hole indicating a reference of the body.
  • Another aspect of the invention is characterized in that the thin film adhesive tape 2a and the ferromagnetic material 820a are replaced by a magnetic sheet to constitute the cover means.
  • one side of the magnetic sheet is coated with a pressure-sensitive adhesive is preferably attached to the upper body (1).
  • the permanent magnet 820b in the lower body 2 acts on the magnetic sheet on the upper body 1 so that the upper body 1 and the lower body 2 are coupled by a magnetic force to form a single body. (100).
  • the magnetic sheet serves as a thin film adhesive tape and a ferromagnetic material at the same time. That is, the magnetic sheet has an advantage of providing the assembly means and the cover means of the upper body 1 and the lower body 2 at the same time.
  • the magnetic sheet is preferably a combination of a cushion rubber material and magnetic powder in the form of a thin film.
  • the cushion rubber material increases the adhesion when the upper body 1 and the lower body 2 are coupled by magnetic force.
  • the magnetic sheet is black, the absorbance of the laser light is high, which is very easy to construct the laser burst valve.
  • the magnetic sheet is preferably in the form of a thin film having a thickness of 0.05 mm to 0.1 mm.
  • FIG 19 shows one embodiment of the opening means using an electromagnet board.
  • the body 100 is attached to and detached from the seating portion 332b of the electromagnet board 330. It is preferred to follow the shape of the body 100 with a seating edge 332a.
  • the body 100 Before the lower body 2 is separated from the body 100, the body 100 is seated on the seating portion 332b of the electromagnet board 330.
  • the body 100 unlike the arrangement at the time of cultivation (see Fig. 16) as shown in Figure 17, it is preferred to seat by changing the top and bottom arrangement of the lower body and the upper body.
  • Reference numeral 182 denotes a fixed table for placing the body 100, and is top loaded onto the fixed table through the center void 170. It is shown that the body has a groove 182b to be mechanically engaged with the fixed table 182.
  • the community gathering process for identification experiments proceeds as follows. First, the body 100 is fitted into the groove 182b, and then the power button 331 is turned on, and then separated from the body 100 into the lower body 2 to collect a sample of the suspected colony from the medium 80. . This collected community will be used for identification experiments. After the power button 331 is off (off), the lower body 2 is coupled to the upper body in accordance with the reference pillar 334. Thereafter, when the knobs 182b and the knobs are pressed, the central void 170 fastened to the groove 182b is released to remove the body 100 from the electromagnet board 330. In addition, the rim groove 333 on the seating edge 332a allows the body 100 to be easily pulled out of the electromagnet board 330.
  • the reference column 334 is fitted to fit the reference hole 335 to set the standard when the body is mounted on the electronic board and recombination of the upper body and the lower body.
  • FIG. 20 shows an embodiment of an oxygen supply valve for supplying oxygen to the aerobic microorganisms in the medium chamber.
  • the oxygen supply valve is composed of a hole (10b) for allowing the entry and exit of oxygen, the connecting passage 135 and the stopper (520a) connected to the medium chambers (132a to 132d).
  • the body 100 When using the microbial analysis device, the body 100 is rotated at a high speed, at which time the stopper 520a beats the adhesive strength of the adhesive means 521a by the strong centrifugal force acting on the stopper 520a and leaves the pores ( 10b) is opened.
  • the hole 10b is closed by the adhesive force (or the pressing force) between the stopper 520a and the bonding means 521a, and the stopper 520a is attached by the centrifugal force. It is characterized in that it is opened while leaving the adhesive strength (adhesive strength) with the means 521a.
  • the adhesive means 521a is preferably a double-coated tape or a rubber coating material having a cushion.
  • the plug is preferably a solid thin film cylinder. In this case, only the upper surface of the stopper was brought into close contact with the hole 10b with a double-sided tape 521a.
  • the portion of the pores 10b is preferred to use as a cushioned rubber or silicone material to increase the closure of the pores 10b.
  • the stopper may be a permanent magnet of a thin film cylindrical shape and a ferromagnetic material may be used as the stopper.
  • the hole is closed by the attraction force of the stopper and the ferromagnetic material, and the stopper 520a overcomes the attraction force and escapes due to the strong centrifugal force acting on the stopper by the high-speed rotation of the body 100 during use. Is opened.
  • 21 is an embodiment of a microbial analysis apparatus using a test strip.
  • Reference numeral 145 denotes a test strip using a membrane.
  • test strip is preferably immobilized with an antibody on a Nitrocellurose membrane or a PVDF membrane, which is referred to as a test line hereinafter.
  • the antibody of the test line is preferably an antibody that specifically binds to at least one selected from Salmonella, Listeria, and O157.
  • the test strip also preferably includes a sample pad 41a, a conjugate pad 41b, and an absorbent pad 41c.
  • the conjugate pad is preferably one in which a gold conjugate, which is complexed with gold particles, is deposited in a lyophilized form.
  • the test strip is characterized in that the reaction test by immunochromatography.
  • the immunochromatography method is a combination of immunochemistry and chromatography (Chromatogrphic Assay), the specific immunoreactivity of the antibody to the antigen, the color development characteristics of the colloidal gold and porous membrane (Porous) It is a test method that applies the movement of molecules by capillary phenomenon of membrane).
  • the test strip may further include a reference line, and the reaction concentration of the reference line may be set to a cutoff value to facilitate the discrimination of negative or positive reactions.
  • Qualitative or quantitative analysis may be performed based on the difference or ratio of the reaction intensity between the reference line and the test line.
  • a sample injection port 121 for injecting a sample
  • a prep chamber 130 in which an antibody is immobilized to perform a specific binding reaction with the sample injected from the sample injection port; After sending the waste which did not bind with the antibody in the prep chamber to the trech chamber 133, and sending the microorganism that caused the reaction with the antibody to the enrichment chamber 131, papain digest (papain).
  • digestion digestion chamber 134 containing reagents for carrying out mecaptoethanol reduction or pepsin digestion;
  • An enrichment chamber 131 for storing a liquid culture medium for enriching microorganisms transferred from the preparation chamber 130 by the digestion;
  • a strip chamber 132 including a test strip 145 to which at least one antibody for immobilizing an antibody antigen reaction against the enriched microorganism is immobilized;
  • a trace chamber 133 for collecting debris from the strip chamber 132; And valves (64, 65, 66, 68) for controlling liquid movement between the chambers;
  • a flow path (channel) for providing a movement path of the specimen; And a rotatable body 100 in which the flow path, the valve and the chamber are integrated.
  • the antibody is cleaved by the reagent that performs pepsin digestion, is separated from the preparation chamber 130, and transferred to the enrichment chamber 131.
  • the body 100 may include one or more strip chambers 132, and may be modified to independently analyze different microorganisms.
  • the body 100 may include one or more strip chamber 132 and the preparation chamber 130, it may be modified to independently analyze different microorganisms.
  • the preparation chamber 130 is characterized in that it comprises magnetic beads (magnetric bead) or gold coated magnetic beads.
  • the antibody is immobilized on the surface of the magnetic beads.
  • the magnetic beads are preferably of superparamagnetic or ferromagnetic properties. It is also preferred that the diameter of the outlet channel 130f of the prep chamber 130 is smaller than the diameter of the magnetic bead so that the magnetic bead stays in the prep chamber. In the present invention, the diameter of the magnetic beads is preferably 0.1um to 10um.
  • the movable sensor 5b is characterized in that the image sensor 103 performs a "search process of the stream chamber" before the image of the strip chamber 132 is taken.
  • a permanent magnet 5c is preferably provided on the body 100 to facilitate optical alignment between the image sensor 103 and the strip chamber 132. .
  • Reference numeral 170 denotes a disk gap.
  • Reference numeral 335 denotes a reference hole indicating a reference of the body.
  • Reference numeral 188 denotes the above-described memory embedded wireless RF IC or electronic tag device.
  • a solid medium or agar medium may be used instead of the test strip in the strip chamber.
  • the microorganisms specifically bound to the antibody in the preparation chamber 130 is transferred to the enrichment chamber 131, and then cultured on the solid medium or agar medium, so that the reliability of separation culture is greatly improved.
  • FIG. 21 shows that only antibodies having a specific binding to the microorganisms are cultured in the enrichment chamber 131, thereby greatly increasing the selectivity for the microorganisms, and thus performing immunological identification tests collectively.
  • One embodiment is provided that provides advantages.
  • the microorganism analyzing apparatus of the present invention and an analysis method using the same are suitable for a thin film type apparatus for diagnosing and detecting a small amount of microorganisms present in a sample for contaminants such as food poisoning test, food microbial test, and water quality.

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Abstract

The present invention relates to a microorganism analyzer which can detect a microorganism present in food, milk, dairy products, agricultural and marine products, beverages and environmental samples by using a biochemical reaction between an enzyme contained in a microorganism and the sugar contained in at least one medium selected from the group of chromogenic mediums consisting of an agar medium, a differential medium and a selective medium, wherein the chromogenic medium shows a distinctive color according to a specific bacterial culture, and an analysis method using the same. More specifically, the present invention relates to a microorganism analyzer comprising: a specimen injection hole for injecting a specimen; a sampler which collects a specimen from an area to be examined and injects the collected specimen into the specimen injection hole; a preparation chamber for temporarily storing the specimen injected from the specimen injection hole; an enrichment chamber which comprises a liquid culture medium to obtain the specimen from the preparation chamber and to enrich a microorganism present in the specimen; one or more culture medium chambers which provide a culture medium for producing nutrients to the enriched microorganism; a trash chamber for collecting remnants and an excess of specimens; a valve for moving fluid and the specimen between the chambers; a channel providing a moving pathway of the specimen; and a rotatable disk-shaped body in which the channel, the valve and the chambers are integrated, and an analysis method using the same.

Description

미생물 분석 장치 및 이를 이용한 분석 방법Microbial analysis apparatus and analysis method using the same
본 발명은 배지에 들어있는 당과 미생물에 들어있는 효소의 생화학적 반응에 의해 특정균의 배양 상태에 따라 특유의 색깔을 띠는 크로모제닉 배지(Chromogenic medium), 아가(agar) 배지 및 선택 배지(selective medium) 중 어느 하나 이상을 이용하여, 식품, 우유, 유제품, 농수산물, 음료 및 환경 시료 내에 존재하는 미생물을 탐지할 수 있는 미생물 분석 장치 및 이를 이용한 분석 방법에 관한 것이다.According to the present invention, a chromogenic medium, agar medium, and selective medium having a specific color according to the culture state of a particular bacterium by the biochemical reaction of a sugar in a medium and an enzyme in a microorganism. The present invention relates to a microbial analysis apparatus capable of detecting microorganisms present in food, milk, dairy products, agricultural products, beverages, and environmental samples using any one or more of the selective medium, and an analysis method using the same.
구체적으로, 살모넬라(Salmonella), 리스테리아(Listeria), 대장균군, 일반세균, 대장균(E.Coli), O157, 비브리오(vibrio) 등의 미생물 존재를 검출하기 위해 미생물의 증균, 도포(접종), 배양, 검출 등의 일련의 공정을 디스크에 집적화하여 특정균이 배양 상태에 따라 특유의 색깔을 띠는 크로모제닉 배지, 아가 배지 및 선택 배지 중 어느 하나 이상에 의해 검체 내 미생물의 존재 여부를 확인하고 콜로니(colony)를 계수(count)할 수 있는 미생물 분석 장치 및 이를 이용한 분석 방법에 관한 것이다.Specifically, in order to detect the presence of microorganisms such as Salmonella, Listeria, Escherichia coli, E. coli, E. coli, O157, and Vibrio, microbial enrichment, application (inoculation), culture Integrate a series of processes, such as detection and detection, on the disc to determine the presence of microorganisms in the sample by any one or more of the chromogenic medium, agar medium, and selection medium in which a particular bacterium has a specific color according to the culture state. The present invention relates to a microbial analysis apparatus capable of counting colonies and an analysis method using the same.
특히, 식품, 수질, 환자 등의 검사 대상물을 크로모제닉 배지, 감별 배지(Differential medium) 혹은 선택 배지에 접종하여 배양한 후 증식된 균집락의 색을 보고 균종을 감별한다.In particular, after inoculating food, water, patient, and the like in a chromogenic medium, differential medium or selective medium, the cultures are differentiated by seeing the color of the grown colonies.
본 발명의 미생물 분석 장치 및 이를 이용한 분석 방법은 식중독균검사, 식품 미생물 검사, 수질 등의 오염 물질 검사를 위한 검체 내에 존재하는 소량의 미생물을 진단 및 탐지하는 박막형 장치에 적합하다. The microorganism analyzing apparatus of the present invention and an analysis method using the same are suitable for a thin film type apparatus for diagnosing and detecting a small amount of microorganisms present in a sample for contaminants such as food poisoning test, food microbial test, and water quality.
최근까지 검체 내 소량의 미생물을 탐지를 위한 분석 과정은 백금이(plantinum loop)를 멸균하고, 멸균된 백금이를 이용해 액체 배양액에서 접종원(inoculum)을 수작업을 통해 취하고, 페트리디쉬(perti dish)의 뚜껑을 비스듬히 열고 백금이를 넣고, 백금이와 배지 표면이 서로 수평이 되도록 한후, 수차례 왕복하는 스트리킹(streaking)을 실시하여 접종원을 배지에 접종한다. 이후 약 37℃ 인큐베이터(Incubator)에서 약 24 내지 48시간 동안 배양하고, 미생물 존재 여부를 확인하는 과정을 거치는 것이 일반적이었다. Until recently, analytical procedures for detecting small amounts of microorganisms in samples were sterilized with platinum loops, manually taken inoculum from liquid cultures using sterilized platinum beads, and the preparation of petri dishes. The lid is opened at an angle, the platinum is added, the platinum and the surface of the medium are horizontal to each other, and several times of reciprocating streaking is performed to inoculate the inoculum into the medium. Since incubation in about 37 ℃ incubator (Incubator) for about 24 to 48 hours, it was common to go through the process of checking the presence of microorganisms.
따라서, 기존의 미생물 분석 과정은 백금이 멸균, 스트리킹 접종과 같은 수작업으로 인해 이들 과정 중에 미생물의 오염에 의해 데이터가 틀릴 수 있을 뿐만 아니라, 고도의 전문가만이 이러한 작업을 수행할 수 있다는 문제점이 있었다.Therefore, the existing microbial analysis process has a problem that not only the data may be wrong due to microbial contamination during these processes due to manual operations such as platinum sterilization and streaking inoculation, but only a highly expert expert can perform this task. .
따라서, 이러한 문제를 극복하기 위해 미생물의 증균, 도포(또는 접종), 배양, 검출 등의 일련의 공정을 자동화할 수 있는 더욱 간단한 미생물 분석 장치 및/또는 방법이 절실히 필요한 실정이다.Therefore, there is an urgent need for a simpler microbial analysis apparatus and / or method capable of automating a series of processes such as enrichment, application (or inoculation), culture, and detection of microorganisms to overcome these problems.
본 발명은 상술된 문제점을 해결하기 위해 안출된 것으로서, 본 발명의 목적은, 검체를 주입하기 위한 검체 주입구; 검사부위로부터 검체를 수집하고 수집된 검체를 상기 검체 주입구에 주입하기 위한 샘플러(sampler); 상기 검체 주입구로부터 주입된 검체를 일시 저장하기 위한 프렙 챔버; 상기 프렙 챔버로 부터의 검체를 취해, 검체 내에 존재하는 미생물을 증균하기 위한 액체 배양액(liquid culture medium)을 포함하는 증균 챔버; 상기 증균된 미생물에 대해 영양분을 제공하기 위한 적어도 하나 이상의 배지 챔버; 찌꺼기 및 과잉 검체를 모으기 위한 트레쉬 챔버; 및 상기 챔버들 사이에 유체 및 검체를 이동시키기 위한 밸브; 상기 검체의 이동 경로를 제공하는 유로(채널); 및 상기 유로, 밸브 및 챔버가 집적화된 회전 가능한 몸체를 구비한 미생물 분석 장치 및 이를 이용한 분석 방법을 제공하는 것이다. The present invention has been made to solve the above-described problems, the object of the present invention, a sample injection port for injecting a sample; A sampler for collecting a sample from an inspection site and injecting the collected sample into the sample inlet; A prep chamber for temporarily storing a sample injected from the sample inlet; An enrichment chamber which takes a sample from the prep chamber and includes a liquid culture medium for enriching the microorganisms present in the sample; At least one medium chamber for providing nutrients to the enriched microorganism; A trech chamber for collecting debris and excess sample; And a valve for moving fluid and sample between the chambers; A flow path (channel) for providing a movement path of the specimen; And it provides a microbial analysis device having a rotatable body in which the flow path, valve and chamber are integrated and an analysis method using the same.
또한, 본 발명의 다른 목적은, 검체 시료를 주입하기 위한 검체 주입구; 검사부위로부터 검체를 수집하고 검체에 존재하는 미생물을 증균하기 위한 액체 배양액을 포함하고 검체를 상기 검체 주입구에 주입하기 위한 샘플러; 상기 샘플러로 부터 주입된 검체를 일시 저장하기 위한 프렙 챔버; 상기 프렙 챔버로부터의 검체를 취하는 동시에, 상기 검체 내의 미생물에 영양분을 제공하기 위한 배지 챔버; 찌꺼기 및 과잉 검체를 모으기 위한 트레쉬 챔버; 및 상기 챔버들 사이에 유체 및 검체를 이동시키기 위한 밸브; 상기 검체의 이동 경로를 제공하는 유로(채널); 및 상기 유로, 밸브 및 챔버가 집적화된 회전 가능한 몸체를 구비한 미생물 분석 장치 및 이를 이용한 분석 방법을 제공하는 것이다. In addition, another object of the present invention, a sample injection port for injecting a sample sample; A sampler for collecting a sample from a test site and including a liquid culture solution for enriching microorganisms present in the sample and injecting the sample into the sample inlet; A prep chamber for temporarily storing a sample injected from the sampler; A medium chamber for taking a sample from the prep chamber and providing nutrients to the microorganisms in the sample; A trech chamber for collecting debris and excess sample; And a valve for moving fluid and sample between the chambers; A flow path (channel) for providing a movement path of the specimen; And it provides a microbial analysis device having a rotatable body in which the flow path, valve and chamber are integrated and an analysis method using the same.
또한, 본 발명의 다른 목적은, (a)샘플러를 이용해 프렙 챔버에 검체를 주입하는 단계; (b) 상기 프렙 챔버 내의 검체를 배지 챔버로 이송하는 단계; (c) 배지 챔버로 이송된 검체를 가지고 배지 챔버 내의 배지 표면에 도포 접종하는 단계; (e) 도포 접종 후 잉여 검체를 트레쉬 챔버에 모으는 세정 단계를 포함하는 본 발명에 따른 미생물 분석장치를 이용한 분석 방법을 제공하는 것이다.In addition, another object of the present invention, (a) injecting a sample to the prep chamber using a sampler; (b) transferring the sample in the preparation chamber to the media chamber; (c) applying and inoculating the medium surface in the medium chamber with the sample transferred to the medium chamber; (e) It provides an analysis method using a microbial analysis apparatus according to the present invention comprising a cleaning step of collecting the surplus specimen in the trech chamber after application inoculation.
본 발명은 검체를 주입하기 위한 검체 주입구; 검사부위로부터 검체를 수집하고 수집된 검체를 상기 검체 주입구에 주입하기 위한 샘플러(sampler); 상기 검체 주입구로부터 주입된 검체를 일시 저장하기 위한 프렙 챔버; 상기 프렙 챔버로 부터의 검체를 취해, 검체 내에 존재하는 미생물을 증균하기 위한 액체 배양액(liquid culture medium)를 포함하는 증균 챔버; 상기 증균된 미생물에 대해 영양분을 제공하기 위한 적어도 하나 이상의 배지 챔버; 찌꺼기 및 과잉 검체를 모으기 위한 트레쉬 챔버; 및 상기 챔버들 사이에 유체 및 검체를 이동시키기 위한 밸브; 상기 검체의 이동 경로를 제공하는 유로(채널); 및 상기 유로, 밸브 및 챔버가 집적화된 회전 가능한 몸체를 구비하는 것을 특징으로 한다. The present invention is a sample injection port for injecting a sample; A sampler for collecting a sample from an inspection site and injecting the collected sample into the sample inlet; A prep chamber for temporarily storing a sample injected from the sample inlet; An enrichment chamber containing a sample from the prep chamber and containing a liquid culture medium for enriching the microorganisms present in the sample; At least one medium chamber for providing nutrients to the enriched microorganism; A trech chamber for collecting debris and excess sample; And a valve for moving fluid and sample between the chambers; A flow path (channel) for providing a movement path of the specimen; And a rotatable body in which the flow path, the valve and the chamber are integrated.
이하, 몸체를 디스크와 혼용한다.Hereinafter, the body is mixed with the disk.
상기 몸체는 상부 몸체와 하부 몸체로 구성되며 결합하여 하나의 몸체를 이루는 것이 선호된다. 상기 샘플러는 수집된 검체로부터 미생물을 추출하기 위한 미생물 추출액을 포함하는 것이 선호된다. The body is composed of an upper body and a lower body and is preferably combined to form a body. The sampler preferably comprises a microbial extract for extracting microorganisms from the collected samples.
본 발명의 또 다른 측면은 상기 샘플러는 멸균백(sterilization pack)에서 액체 증균 배지에 의해 배양된 검체를 샘플링하기 위한 피펫(pipette)이 선호된다. In another aspect of the invention, the sampler is preferably a pipette for sampling a sample incubated with liquid enrichment medium in a sterilization pack.
본 발명에서, 상기 액체 백양액 또는 액체 증균 배지는 mEC Broth, RV(Rappaport Vassiliadis) Broth, Listeria Enrichment Broth, UVM-modified Listeria Enrichment Broth, Fraser Broth, TSB(Tryptone Sonya Broth), PBS (phosphate buffered saline), Peptone water, lactose Broth, desoxycholate lactose Broth 중 선택된 어느 하나인 것이 선호된다.In the present invention, the liquid white liquor or liquid enrichment medium is mEC Broth, RV (Rappaport Vassiliadis) Broth, Listeria Enrichment Broth, UVM-modified Listeria Enrichment Broth, Fraser Broth, Tryptone Sonya Broth (TSB), PBS (phosphate buffered saline) Preference is given to any one selected from among Peptone water, lactose broth and desoxycholate lactose broth.
상기 배지 챔버는 XLD Agar, MacConkey Agar, Desoxycholate, Citrate Agar, Bismuth Sulfite Agar, Nutrient Agar, TSI Agar, Sorbitol MacConkey Agar, Oxford Agar, PALCAM Agar, LPM Agar, Mannitol Salt Agar, Baird-Parker Agar, MYP Agar, 크로모제닉 배지, 고체 배지 중 선택된 적어도 어느 하나 이상을 포함되는 것이 선호된다.The medium chamber is XLD Agar, MacConkey Agar, Desoxycholate, Citrate Agar, Bismuth Sulfite Agar, Nutrient Agar, TSI Agar, Sorbitol MacConkey Agar, Oxford Agar, PALCAM Agar, LPM Agar, Mannitol Salt Agar, Baird-Parker Agar, MYP Agar, It is preferred to include at least one selected from chromogenic media and solid media.
본 발명의 또 다른 측면은 검체 시료를 주입하기 위한 검체 주입구; 검사부위로부터 검체를 수집하고 검체에 존재하는 미생물을 증균하기 위한 액체 배양액을 포함하고 검체를 상기 검체 주입구에 주입하기 위한 샘플러(sampler); 상기 샘플러로 부터 주입된 검체를 일시 저장하기 위한 프렙 챔버; 상기 프렙 챔버로 부터의 검체를 취하는 동시에, 상기 검체 내의 미생물에 영양분을 제공하기 위한 배지 챔버; 찌꺼기 및 과잉 검체를 모으기 위한 트레쉬 챔버; 및 상기 챔버들 사이에 유체 및 검체를 이동시키기 위한 밸브; 상기 검체의 이동 경로를 제공하는 유로(채널); 및 상기 유로, 밸브 및 챔버가 집적화된 회전 가능한 몸체를 구비하는 것을 특징으로 한다. Another aspect of the invention the sample inlet for injecting a sample sample; A sampler for collecting the sample from the test site and including a liquid culture solution for enriching the microorganisms present in the sample and injecting the sample into the sample inlet; A prep chamber for temporarily storing a sample injected from the sampler; A medium chamber for taking a sample from the prep chamber and providing nutrients to the microorganisms in the sample; A trech chamber for collecting debris and excess sample; And a valve for moving fluid and sample between the chambers; A flow path (channel) for providing a movement path of the specimen; And a rotatable body in which the flow path, the valve and the chamber are integrated.
본 발명의 상기 몸체는 동정(identification)실험을 허여하는 개폐가 가능한 커버 수단을 더 포함할 수 있다. 이러한 커버 수단을 통해 배지 챔버에서 의심되는 군락의 샘플을 채집할 수 있다.The body of the present invention may further include a cover means capable of opening and closing allowing identification experiment. This cover means allows the collection of a sample of suspected colonies in the media chamber.
본 발명에 있어서, "동정실험"이란 배지를 통해 균을 배양하고 그 중에 의심되는 군락에 대해 샘플을 취해 다시 생화학적, 혈청학적, 유전학적 실험 방법 등을 통해 확인하는 실험을 의미한다.In the present invention, "identification experiment" refers to an experiment in which bacteria are cultured through a medium, samples of suspected colonies are sampled, and confirmed through biochemical, serological and genetic experimental methods.
본 발명의 상기 몸체는 호기성 미생물에 산소를 공급하기 위한 산소 공급용 밸브를 더 포함할 수 있다. The body of the present invention may further include an oxygen supply valve for supplying oxygen to the aerobic microorganisms.
상기 배지 챔버 내에서 호기성 미생물이 잘 자라기 위해서는 배양기간 동안 산소가 원활하게 공급되어야 한다. 그러나 배지 챔버 내의 배지의 건조 및 다른 미생물로부터의 오염을 막기 위해서는 유통기간 동안 상기 몸체는 밀폐되어야 한다. In order for the aerobic microorganism to grow well in the medium chamber, oxygen should be supplied smoothly during the culture period. However, the body must be sealed during the shelf life to prevent drying of the medium in the medium chamber and contamination from other microorganisms.
따라서 상기 산소 공급용 밸브는 유통기간 동안에는 밀폐되어 있다가, 배양 시작시 개방되게 함으로써, 유통기간 동안에 배지 챔버 내의 배지의 건조를 막는 동시에, 배양 동안 호기성 미생물에 산소를 공급하여 준다. Therefore, the oxygen supply valve is closed during the distribution period, and is opened at the start of the culture, thereby preventing the drying of the medium in the media chamber during the distribution period and supplying oxygen to the aerobic microorganisms during the culture.
고체 배지에는 다량의 수분이 포함되어 있다. 때문에 똑바로 배양하면 증발현상이 일어나 상부 몸체 천정에 습기가 차고 습기가 많아지고 미생물의 배양 상태를 망가뜨릴 가능성이 있을 뿐만 아니라, 금방 배양 동안 배지가 말라버릴 수 있다.The solid medium contains a large amount of water. Therefore, if cultured straight, evaporation can occur, and the upper body ceiling may be moist and humid, and the culture state of the microorganisms may be damaged, and the medium may dry out immediately during the culture.
본 발명의 상기 배지 챔버 내의 배지는 고체 배지가 선호되며, 이 경우 위 몸체 쪽에 배지가 설치되는 것을 특징으로 한다.The medium in the medium chamber of the present invention is preferably a solid medium, in which case the medium is installed on the body side.
본 발명의 미생물 분석장치는 미생물의 관찰하기 위한 현미경 센서와 현미경 센서로부터의 이미지를 처리하고 콜로니를 계수하기 위한 중앙제어장치를 내장하는 것이 선호된다. 미생물 배양은 통상적으로 24시간 내지 48시간의 배양 시간이 요구된다. 그러나 배지 챔버 내의 미생물을 현미경 센서로 보면 미생물의 성장을 좀더 일찍 관찰할 수 있을 뿐만 아니라, 시간별 콜로니의 크기 변화로 균주의 생존 여부를 확인할 수 있다. 시간증가에 따라 콜로니의 사이즈가 커지면 살아있는 그 군집은 살아있는 세포로 판단할 수 있다.The microorganism analyzing apparatus of the present invention preferably includes a microscopic sensor for observing microorganisms and a central control unit for processing images from the microscopic sensors and counting colonies. Microbial culture usually requires a culture time of 24 to 48 hours. However, by viewing the microorganisms in the medium chamber under a microscope sensor, not only can the microorganisms grow earlier, but also the survival of the strains can be confirmed by changing the size of colonies over time. As colonies grow in size over time, the living colony can be considered a living cell.
본 발명에 있어서 상기 현미경 센서는 20배 내지 100배의 광학줌(potical zoom)을 갖는 것이 선호된다.In the present invention, it is preferable that the microscope sensor has an optical zoom of 20 to 100 times.
본 발명의 상기 샘플러는 균질화(stomaching) 수단을 더 포함할 수 있다. 본 발명에 있어서, 상기 미생물 분석 장치는 미생물 증균의 최적 조건을 제공키 위한 온도 제어 장치 내지 항온기를 더 구비하는 것을 특징으로 한다. 본 발명에 있어서, 상기 미생물 추출액 혹은 희석액은 증류수(DeIonize water, DI water), PBS(Phosphate Buffered Saline)가 선호된다.The sampler of the present invention may further comprise a homogenizing means. In the present invention, the microbial analysis device is characterized in that it further comprises a temperature control device to a thermostat for providing optimum conditions of microbial enrichment. In the present invention, the microbial extract or diluent is preferably distilled water (DeIonize water, DI water), PBS (Phosphate Buffered Saline).
본 발명에 있어서, 상기 액체 배양액은 펩톤 수(peptone water), 육즙(meat extract), 효모추출물(yeast extract)이 선호된다.In the present invention, the liquid culture solution is preferably peptone water, meat extract, yeast extract.
본 발명의 미생물 분석 장치의 몸체는 통상적인 CD-ROM, DVD 등과 같은 디스크가 선호된다.The body of the microbial analysis apparatus of the present invention is preferably a disk such as a conventional CD-ROM, DVD and the like.
상기 몸체의 두께는 2mm 내지 100mm가 선호되며, 지름은 30mm~120mm가 선호되며，120mm，80mm，혹은 32mm인 원형 디스크가 보다 선호된다．The thickness of the body is preferably 2mm to 100mm, the diameter of 30mm ~ 120mm is preferred, the round disk of 120mm, 80mm, or 32mm is preferred.
상기 배지 챔버는 서로 다른 종류의 미생물에 대해 선택적으로 반응하는 선택 배지(selective medium)로 구성되어 복수의 미생물을 동시에 분석하는 것이 선호된다. The medium chamber is composed of a selective medium that selectively reacts with different types of microorganisms, and therefore, it is preferable to simultaneously analyze a plurality of microorganisms.
상기 배지 챔버는 플로로제닉 배지, 크로모제닉 배지, 감별 배지, 선택 배지 중 선택된 한 개 이상의 배지로 구성되어 복수 개의 미생물을 동시에 분석하는 것을 특징으로 한다. The medium chamber is composed of one or more medium selected from phlorogenic medium, chromogenic medium, differentiation medium, selection medium, characterized in that for analyzing a plurality of microorganisms at the same time.
본 발명에 있어서, "선택 배지"란, 특정 미생물의 생장을 도모하고 다른 미생물의 생장을 억제함으로써 원하는 미생물만 선택적으로 배양하는데 사용하는 배지로서, 예를 들어, MacConkey Agar배지, Salmonella-shigella 배지 등을 사용할 수 있다.In the present invention, the "selective medium" is a medium used for selectively cultivating only desired microorganisms by promoting the growth of specific microorganisms and inhibiting the growth of other microorganisms, for example, MacConkey Agar medium, Salmonella-shigella medium and the like. Can be used.
본 발명에 있어서, "플로로제닉 배지"란, 배지에 들어있는 당과 미생물에 들어있는 효소의 생화학적 반응에 의해 특정균의 배양 상태에 따라 특유의 형광 빛(fluorescent light)을 나타내는 배지이다. In the present invention, the "fluorogenic medium" refers to a medium that exhibits a unique fluorescent light depending on the culture state of a specific bacterium by the biochemical reaction of a sugar in a medium and an enzyme in a microorganism.
본 발명에 따른 미생물 분석 장치는 살모넬라 (Salmonella), 바실러스 (Bacillus), 리스테리아(Listeria), 비브리오(vibrio), Campylobacter S.aureus, 대장균군(E.Coliform), 대장균(E.coli), Shigella, Legionella Enterobacter sakazakii, Citrobacter, Preteus, Citrobacter, MRSA, E.coli O157, E.coli spp, L.monocytogenes, L.inocua, V.parahaemolyticus, V.vulnificus & V.cholerae, V.alginolyticus, Coliforms, Pseudomonas, Klebsiella, Citrobacter, Enterococcus, PMP group, Staphylococcus saprophyticus, Stapylococcus aureus, S. agalactiae, Candida과 같은 미생물을 탐지하는 것이 선호된다.Microbial analysis apparatus according to the present invention is Salmonella (Salmonella), Bacillus (Bacillus), Listeria, Vibrio, Campylobacter S. aureus, E. Coliform, E. coli, E. coli, Shigella, Legionella Enterobacter sakazakii, Citrobacter, Preteus, Citrobacter, MRSA, E.coli O157, E.coli spp, L.monocytogenes, L.inocua, V.parahaemolyticus, V.vulnificus & V.cholerae, V.alginolyticus, Coliforms, Pseudomonas Microorganisms such as Klebsiella, Citrobacter, Enterococcus, PMP group, Staphylococcus saprophyticus, Stapylococcus aureus, S. agalactiae, Candida are preferred.
이들 미생물은 콜로니 형성시, 배지에 포함된 여러가지 금속성분과 염색약 또는 배지에 들어있는 당과 미생물에 들어있는 효소의 생화학적 반응에 의해 특정균의 배양 상태에 따라 특유의 색깔을 띠는 때문에 콜로니의 색상 판독과 콜로니의 계수에 의해 특정 미생물의 존재 유무 및 미생물의 번식 정도를 정량 분석할 수 있다. 예를 들면, 미생물 E. coli는 빨강색, Streptoccoccus은 청록색, Proteus은 갈색, E.coli O157는 연자주, Coliform는 파랑색을 갖는다.These microorganisms have a unique color depending on the culture state of specific bacteria by colony formation, biochemical reactions of various metals in the medium, dyes or sugars in the medium and enzymes in the microorganisms. Color readings and colony counts allow quantitative analysis of the presence and presence of specific microorganisms. For example, microbes E. coli are red, Streptoccoccus is cyan, Proteus is brown, E. coli O157 is soft purple, and Coliform is blue.
본 발명에 있어서, 밸브는 유통 보관 기간 동안 유공을 폐쇄하기 위한 박막 접착 테이프(thin film adhesive tape)를 유공에 부착하여 완전 폐쇄(closing)하고 디스크의 원심력에 의해 유체에 형성된 수압(hydraulic force)의 힘으로 박막 접착 테이프를 유공으로부터 뜯어내어 유공을 개방(opening)시키는 밸브가 바람직하다. In the present invention, the valve attaches a thin film adhesive tape to the pores to close the pores during the distribution storage period, thereby completely closing the hydraulic pressure formed in the fluid by the centrifugal force of the disk. A valve is preferable in which the thin film adhesive tape is peeled from the pores with a force to open the pores.
이하, 본 발명에서는 상기 디스크의 원심력에 의해 유체 자체에 형성된 유압(hydraulic force)의 힘으로 박막 접착테이프를 유공으로부터 뜯어내어 유공을 개방시키는 밸브를 "수압 버스트 밸브"(hydraulic burst valve)라 칭한다.Hereinafter, in the present invention, a valve for releasing the thin film adhesive tape from the pores and opening the pores by the force of hydraulic force formed in the fluid itself by the centrifugal force of the disk is referred to as a "hydraulic burst valve".
이러한 "수압 버스트 밸브"는 박막 형태의 밸브 구현이 가능할 뿐만 아니라, 박막접착 테이프는 플렉시블(flexible)하므로 온도와 같은 환경적 요소에 따른 팽창과 수축에 잘 적응하기 때문에 유통 보관 기간 중에 액체의 증발 혹은 몸체의 팽창 및 수축에 의한 실링(sealing) 문제가 발생하지 않는 장점이 있다.This "hydraulic burst valve" is not only a valve in the form of a thin film, but also a thin film adhesive tape (flexible), it is well adapted to expansion and contraction according to environmental factors such as temperature, so that the liquid evaporation or There is an advantage that the sealing (sealing) problem due to the expansion and contraction of the body does not occur.
또한, 상기 박막 접착 테이프에 의한 유공 폐쇄시 박막 접착테이프의 접착 면적에 의해 폐쇄 강도(closing strength)를 결정하고, 상기 폐쇄 강도를 극복하는 디스크 회전 속도(원심력) 이상에서 상기 박막 접착테이프가 뜯겨져 유공이 개방된다. In addition, the closing strength is determined by the adhesive area of the thin film adhesive tape when the hole is closed by the thin film adhesive tape, and the thin film adhesive tape is torn apart at a disk rotation speed (centrifugal force) that overcomes the closing strength. The pores open.
또한 상기 박막 접착 테이프는 조사된(irradiated) 레이저 빔의 열에 의해 점착력이 약해져 밸브의 개방을 원하는 경우 유공 부위에 레이저 빔을 가하면 회전력에 의해 박막접착 테이프가 유공으로부터 쉽게 이탈된다. In addition, when the thin film adhesive tape is weakened by the heat of the irradiated laser beam, and the opening of the valve is desired, the thin film adhesive tape is easily detached from the pore by applying the laser beam to the hole.
본 발명의 또 다른 측면은, 상기 밸브는 유통 보관 기간 동안 유공을 폐쇄하기 위한 박막 접착 테이프를 유공에 부착하여 완전 폐쇄하고 레이저의 열에 의해 박막 접착 테이프를 절단하여 유공을 개방시키는 밸브가 바람직하다. According to another aspect of the present invention, the valve is preferably a valve for attaching a thin film adhesive tape to close the pores during the distribution storage period to completely close the open pores and cutting the thin film adhesive tape by the heat of the laser to open the pores.
이하, 본 발명에서는 상기 레이저의 열에 의하여 박막 접착 테이프를 절단함으로써 유공을 개방시키는 밸브를 "레이저 버스트 밸브"(laser burst valve)라 칭한다.Hereinafter, in the present invention, a valve that opens the pores by cutting the thin film adhesive tape by the heat of the laser is referred to as a "laser burst valve".
이때, 상기 박막 접착 테이프의 색은 레이저 빔을 잘 흡수하기 위해, 검정색인 것이 선호된다. At this time, the color of the thin film adhesive tape is preferably black, in order to absorb the laser beam well.
본 발명의 또 다른 측면은, 밸브는 유공과 마개 간의 접착력 혹은 결합력에 의해 유공을 밀폐함으로써 유통보관 기간 동안 유공을 차단하고, 사용시 디스크의 고속회전에 의한 원심력에 의해 상기 마개가 유공에서 이탈하여 유공이 개방될 수 있는 마개 밸브를 이용한 미생물 분석 장치 및 이를 이용한 분석 방법을 제공하는데 그 목적이 있다. In another aspect of the present invention, the valve seals the pores by the adhesive force or the binding force between the pores and the stopper to block the pores during the distribution storage period, and the stopper is separated from the pores by the centrifugal force by the high-speed rotation of the disc in use. It is an object of the present invention to provide a microbial analysis device using the open stopper valve and an analysis method using the same.
상기 마개는 열에 의해 녹는 점착제 재료이거나, 마개와 유공과 접착을 위해 상기 유공 면에 점착제에 의해 코팅된 것이 선호된다. The stopper is preferably an adhesive material which melts by heat or is coated with an adhesive on the face of the hole for adhesion with the stopper and the hole.
또한 상기 마개의 주변에 레이저 빔을 흡수키 위한 레이저 빔 흡수 물질이 코팅되는 것이 선호된다. 이러한 레이저 빔 흡수 물질은 레이저 빔 조사(illumination)시 레이저 빔을 흡수하여 열을 쉽게 발생시키는 특성이 있다.It is also preferred to coat a laser beam absorbing material around the stopper to absorb the laser beam. Such a laser beam absorbing material has a property of easily generating heat by absorbing a laser beam during laser beam irradiation.
또한 상기 점착제는 조사된(irradiated) 레이저 빔의 열에 의해 점착력이 약해지든지 녹는 것이 선호된다. 따라서 밸브의 개방을 원할 시 마개에 레이저 빔을 가하면 회전력에 의해 마개가 쉽게 이탈된다. It is also preferred that the pressure sensitive adhesive is weakened or melted by the heat of the irradiated laser beam. Therefore, if you want to open the valve, apply a laser beam to the stopper is easily detached by the rotational force.
이하, 본 발명에서는 상기 디스크의 원심력에 의해 상기 유공을 막고 있는 마개가 마개와 유공 간의 접착력 혹은 결합력을 이겨내어 유공으로부터 이탈하여 유공을 개방시키는 밸브를 "마개 버스트 밸브"(stopple burst valve)라 칭한다. Hereinafter, in the present invention, a valve in which the stopper blocking the hole by the centrifugal force of the disk overcomes the adhesive or bonding force between the stopper and the hole and is separated from the hole so as to open the hole. The stopper burst valve is referred to as a "stopple burst valve". .
상기 "마개 버스트 밸브"는 구슬 혹은 박막 원기둥 모양의 마개가 선호되나, 상술된 기능 또는 작용을 수행할 수 있는 한 그 형상은 특별히 제한되지 않음을 유의한다.Note that the "bush burst valve" is preferably a bead or thin film cylindrical stopper, but the shape is not particularly limited as long as it can perform the above-described functions or functions.
본 발명은 또 다른 측면은, 상기 밸브는 자성체로 구성된 구속홈과 자석 밸브 간의 인력에 의해 유공을 밀폐하고, 디스크의 회전에 의한 원심력에 의해 상기 자석 밸브가 구속홈으로부터 이탈하여 유공이 개방될 수 있는 자석 밸브를 이용한 미생물 분석 장치 및 이를 이용한 분석 방법을 제공하는데 그 목적이 있다.According to another aspect of the present invention, the valve seals the oil hole by the attraction force between the constrained groove made of a magnetic material and the magnetic valve, and the magnetic valve may be released from the constrained groove by the centrifugal force by the rotation of the disk to open the oil hole. An object of the present invention is to provide a microbial analysis apparatus using a magnetic valve and an analysis method using the same.
이하, 본 발명에서는 상기 디스크의 원심력에 의해 상기 유공을 막고 있는 자석 밸브가 구속홈과의 인력을 이겨내어 구속홈으로부터 이탈하여 유공을 개방(opening)시키는 밸브를 "자석 버스트 밸브"(magnet burst valve)라 칭한다.Hereinafter, in the present invention, the magnetic valve which blocks the hole by the centrifugal force of the disk overcomes the attraction force with the restraint groove and is separated from the restraint groove to open the hole. It is called).
상기 "자석 버스트 밸브"는 구슬, 박막 원기둥 자석 혹은 박막 사각형 자석이 선호되나, 상술된 기능 또는 작용을 수행할 수 있는 한 그 형상은 특별히 제한되지 않음을 유의한다.Note that the "magnetic burst valve" is preferably a bead, a thin film cylindrical magnet or a thin film rectangular magnet, but the shape is not particularly limited as long as it can perform the above-described functions or functions.
상기 자석 버스트 밸브는 디스크 회전정지시, 구속홈으로부터 이탈되었던 자석밸브는 구속홈과의 인력에 의해 유공을 다시 폐쇄(closing)되는 것이 선호된다. 상기 자석 밸브는 레이저 빔을 조사함으로써 레이저 빔의 열에 의해 감자(demagnetization)가 되어 자력이 약해지는 것이 선호된다. 따라서 밸브의 개방을 원할시 자석 밸브에 레이저 빔을 가하면 회전력에 의해 자석밸브가 쉽게 개방된다.When the magnet burst valve stops rotating the disc, it is preferable that the magnet valve that has been removed from the restraint groove is closed again by the attraction force with the restraint groove. The magnet valve is preferably demagnetized by the heat of the laser beam by irradiating the laser beam, so that the magnetic force is weakened. Therefore, if you want to open the valve and apply a laser beam to the magnet valve, the magnet valve is easily opened by the rotational force.
본 발명은 또 다른 측면은, 상기 밸브는 상부 몸체와 하부 몸체 사이에 형성된 소수성(hydrophobic) 채널에 의해 만들어지는 것을 특징으로 한다. 상기 소수성 채널은 소수성이므로 평상시 유체가 이동하기 어려우며 몸체의 회전시 발생하는 원심력에 의해서만 유체가 채널을 지나갈 수 있다.In another aspect, the invention is characterized in that the valve is made by a hydrophobic channel formed between the upper body and the lower body. Since the hydrophobic channel is hydrophobic, it is difficult for the fluid to move normally and the fluid can pass only through the centrifugal force generated when the body rotates.
상기 소수성 채널은 채널 형상이 설계된 박막 접착 테이프에 의해 상부 몸체와 하부 몸체 접착시 상부 몸체와 하부 몸체 사이에 형성되는 것이 선호된다. 즉, 상기 상부 몸체와 하부 몸체는 박막 접착 테이프에 의해 서로 밀착 부착되어 하나의 몸체를 이루며, 이때 상부 몸체와 하부 몸체 사이에서 박막 접착 테이프가 빠진 부분, 즉 채널 형상에 의해 소수성 박막 채널이 형성된다. 이때 소수성 박막 채널의 높이는 박막 접착 테이프의 두께에 의해 결정된다.Preferably, the hydrophobic channel is formed between the upper body and the lower body when the upper body and the lower body are bonded by a thin film adhesive tape having a channel shape. That is, the upper body and the lower body are closely attached to each other by a thin film adhesive tape to form a single body, wherein a hydrophobic thin film channel is formed by a portion in which the thin film adhesive tape is missing between the upper body and the lower body, that is, the channel shape. . The height of the hydrophobic thin film channel is determined by the thickness of the thin film adhesive tape.
이하, 본 발명에서는 상기 몸체의 원심력에 의해 상기 유체의 이동을 막고 있는 소수성에 대한 저항을 이겨내고 유체가 소수성 박막 채널을 이동하는 밸브를 "소수성 버스트 밸브"(hydrophobic burst valve)라 칭한다.Hereinafter, in the present invention, a valve that overcomes the resistance to hydrophobicity preventing the fluid from moving by the centrifugal force of the body and moves the hydrophobic thin film channel is referred to as a "hydrophobic burst valve".
이하 본 발명에 있어서, "수압 버스트 밸브", "마개 버스트 밸브", "자석 버스트 밸브, "레이저 버스트 밸브" 및 "소수성 버스트 밸브"를 총칭하여 "버스트 밸브(Burst valve)"라 부르기로 한다. Hereinafter, in the present invention, the "hydraulic burst valve", the "bump burst valve", the "magnetic burst valve, the" laser burst valve "and the" hydrophobic burst valve "will be collectively referred to as" burst valve ".
본 발명에 있어서, 바람직하게는 버스트 밸브의 개방은 레이저빔의 의해 발생된 열과 원심력 또는 원심력에 의해 이루어지는 것을 특징으로 한다.In the present invention, preferably, the burst valve is opened by heat generated by the laser beam and centrifugal force or centrifugal force.
레이저 빔 발생 장치로부터의 레이저빔을 버스트 밸브 주위에 조사(irradiation)하여 레이저 빔에 의한 열에 의해 버스트 밸브의 밀폐력 내지 결합력을 약화시켜 원심력에 의해 쉽게 개방될 수 있도록 한다. 이때, 상기 레이저 빔 발생장치는 스라이더(slider)에 탑재되는 것이 선호된다.The laser beam from the laser beam generator is irradiated around the burst valve to weaken the sealing force or coupling force of the burst valve by heat by the laser beam so that it can be easily opened by centrifugal force. In this case, the laser beam generator is preferably mounted on a slider.
특히, 레이저빔을 버스트 밸브 주위에 조사(irradiation)하는 방법은 다음 2가지가 선호된다.In particular, the following two methods of irradiating a laser beam around a burst valve are preferred.
첫번째 방법은 디스크 회전중에 버스트 밸브에 레이저 빔을 조사하기 위해, 해당 버스트 밸브의 반경 위치에 스라이더이동에 의해 레이저 빔 발생장치를 이동시키고, 레이저 빔을 온(On) 시키면, 디스크 회전중 레이저 빔 발생장치와 해당 버스트 밸브와 일치할 때(즉, 마주칠 때)마다, 그 버스트 밸브에 레이저 빔이 조사(irradiation)되며, 이 경우 버스트 밸브에 조사되는 레이저 빔의 량은 디스크의 회전 속도 및 레이저 빔 발생장치의 파워(power)의 함수가 되며 이러한 디스크 회전중 레이저 빔 조사 동작을 본 발명에서는 이하 "스캔닝 빔(scanning Beam)" 동작이라 칭한다. The first method is to move the laser beam generator by moving the laser beam to a radial position of the burst valve in order to irradiate the laser beam to the burst valve during disk rotation. Whenever the generator coincides with (i.e. encounters with) the corresponding burst valve, a laser beam is irradiated to the burst valve, in which case the amount of laser beam irradiated to the burst valve is the rotational speed of the disc and the laser The laser beam irradiation operation during the rotation of the disk, which is a function of the power of the beam generator, is hereinafter referred to as " scanning beam " operation in the present invention.
두번째 방법은 디스크 회전중에 버스트 밸브에 레이저 빔을 조사키 위해, 해당 버스트 밸브의 반경 위치에 스라이더 이동에 의해 레이저 빔 발생장치를 이동시키고, 디스크 회전중 레이저 빔 발생장치와 해당 버스트 밸브와 일치할 때(즉 마주칠 때)마다 레이저 빔을 온(On) 시키고 그 이외는 오프(Off)시킨다. 이러한 레이저 빔 조사 동작을 본 발명에서는 이하 "펄스 빔(pulse Beam)" 동작이라 칭한다. In the second method, the laser beam generator is moved by moving the laser beam to the burst valve at the radial position of the burst valve during disk rotation, and the laser beam generator and the corresponding burst valve are rotated during the disk rotation. The laser beam is turned on every time (ie, encountering) and off otherwise. This laser beam irradiation operation is hereinafter referred to as "pulse beam" operation in the present invention.
본 발명에 있어서, 상기 버스트 밸브의 개방은 상기 "펄스 빔" 동작 혹은 "스캔닝 빔" 동작에 의한 버스트 밸브의 가열 후, 디스크 회전에 의한 것을 특징으로 한다.In the present invention, the opening of the burst valve is characterized by disk rotation after heating of the burst valve by the "pulse beam" operation or the "scanning beam" operation.
본 발명에서, 바람직하게는 미생물 분석장치의 동심원상의 버스트 밸브의 개방은 미생물 분석장치 회전중 유체에 발생한 원심력과 미생물 분석장치 회전중 스라이더(slider)상에 설치된 레이저 빔 발생장치와 해당 동심원상의 버스트 밸브와 일치 할 때 마다 버스트 밸브가 가열되어 개방되는 "펄스 빔" 혹은 "스캔닝 빔" 동작에 의한 것이 선호하며, 이러한 "펄스 빔" 혹은 "스캔닝 빔" 동작은 점도가 높은 유체를 이동시키거나 동심원상의 복수의 버스트 밸브의 동시 개방을 통해 회전 동안 해당 이웃 챔버들로 이동시킬 때 유용한다. 점도가 높은 유체는 원심력이 동반되어야 이동하기 용이하다. 정지중에는 밸브가 개방되어도 점도가 높은 유체는 이웃 챔버로 이동치 않을 위험성이 있어 재현성과 신뢰성에 문제를 일으킬 수 있다.In the present invention, preferably, the opening of the burst valve on the concentric circle of the microbial analyzer is performed by the centrifugal force generated in the fluid during the rotation of the microbial analyzer and the laser beam generator installed on the slider during the rotation of the microbial analyzer and the burst on the concentric circle. Preferred by a "pulse beam" or "scanning beam" operation in which the burst valve is heated to open each time it matches a valve, such a "pulse beam" or "scanning beam" operation causes the high viscosity fluid to move. Or through simultaneous opening of a plurality of burst valves in concentric circles to the corresponding neighboring chambers during rotation. Fluid with high viscosity is easy to move with centrifugal force. Highly viscous fluids may not move to neighboring chambers even when the valve is open during shutdown, which may cause reproducibility and reliability problems.
한편, 배지 챔버의 미생물 여부를 검출하기 위해서는 이미지 센서장치가 필요로 하다. 본 발명에서 상기 디스크는 적어도 하나 이상의 배지 챔버가 동심원상으로 배치되는 것이 선호된다.On the other hand, in order to detect the presence of microorganisms in the medium chamber requires an image sensor device. In the present invention, the disk preferably has at least one medium chamber arranged concentrically.
본 발명의 미생물 분석장치는, 상기 디스크의 배지 챔버를 촬영하기 위한 이미지 센서; 및 상기 디스크를 회전시키기 위한 스핀들 모터(spindle motor)를 포함 한다. Microbial analysis apparatus of the present invention, the image sensor for photographing the medium chamber of the disk; And a spindle motor for rotating the disk.
상기 동심원상의 배지 챔버에 대한 전체 촬영은 스핀들(spindle) 모터를 360도 서행 회전시키면서 이미지 센서에 의해 촬영하든지, 스핀들 모터의 짧은 회전과 중지의 반복 동작을 통해, 정지때 마다 이미지를 얻고 이들을 조합하여 360도 전체 이미지를 얻는다. The entire shooting of the concentric discharge chamber may be performed by an image sensor while rotating the spindle motor 360 degrees slowly, or by repeating the rotation and stopping of the spindle motor repeatedly to obtain an image at each stop and combine them. Get a 360 degree full image.
본 발명에 있어서, 상기 이미지 센서는 배지 챔버 내의 미생물 집락(colony)을 색상별로 분류하고 이를 계수하여 정량분석하는 것을 특징으로 한다. In the present invention, the image sensor is characterized in that the microbial colonies (colony) in the medium chamber by color classification and counting and quantitative analysis.
상기 계수는 배지 챔버 내의 집락을 형성하는 균의 수를 측정하는 것으로 평판계수법(plate count) 또는 집락계수법(colony count)이 선호된다. 상기 계수는 세균수 또는 집락 형성 단위(colony-forming unit. cfu/ml 또는 cfu/g)로 표시한다.The count is a measure of the number of bacteria forming colonies in the medium chamber, and plate count or colony count is preferred. The coefficients are expressed in bacterial counts or colony-forming units (cfu / ml or cfu / g).
본 발명에 있어서, 상기 이미지 센서는 디스크 상의 바코드(barcode)를 판독하는 것을 특징으로 한다.In the present invention, the image sensor is characterized in that reading a barcode (barcode) on the disk.
본 발명에 있어서, 상기 이미지 센서는 디스크 상의 바코드를 판독하여 디스크의 진품 여부를 인증하거나, 해당 바코드의 데이터 및 사용자의 정보(예를 들면, IP 주소, 위치, 전화번호, e_mail 주소, 업체명, 혹은 디스크의 시리얼 번호 등)를 인터넷에 의해 원격 전송하여 서버로부터 디스크의 진품 여부 및 제품 식별 정보를 통보받는 것을 특징으로 한다.In the present invention, the image sensor reads a bar code on the disc to authenticate the authenticity of the disc, or the data of the bar code and the user's information (for example, IP address, location, telephone number, e_mail address, company name, or The serial number of the disk, etc.) is remotely transmitted through the Internet to receive notification of authenticity of the disk and product identification information from the server.
상기 미생물 분석장치는 상기 입출력 장치에 의해 컴퓨터 혹은 인터넷 네트워크에 연결될 수 있다.The microorganism analyzing apparatus may be connected to a computer or an internet network by the input / output device.
디스크가 진품이 아닌 경우 상기 바코드가 서버에 등록되어 있지 않으므로, 중앙 서버로부터 제품 식별정보를 받을 수 없으며, 이 경우 상기 미생물 분석장치는 디스크를 구동할 수 없게 된다. 반면에, 디스크가 진품인 경우 상기 바코드가 중앙 서버에 등록되어 있고, 중앙 서버로부터 제품 식별정보를 수신하게 되면 제품 식별 정보에 해당되는 구동 소프트웨어에 따라 상기 미생물 분석 장치는 디스크를 구동하게 된다. 만약 상기 제품식별 정보에 해당되는 구동 소프트웨어가 컴퓨터 혹은 미생물 분석 장치에 설치되어 있지 않은 경우, 중앙 서버로부터 인터넷 네트워크를 통해 구동 소프트웨어를 다운로드받게 된다.If the disc is not genuine, since the barcode is not registered in the server, product identification information cannot be received from the central server, and in this case, the microbial analyzer cannot drive the disc. On the other hand, when the disc is genuine, the barcode is registered in the central server, and when the product identification information is received from the central server, the microbial analysis apparatus drives the disc according to the driving software corresponding to the product identification information. If the driving software corresponding to the product identification information is not installed in the computer or the microbial analysis apparatus, the driving software is downloaded from the central server through the Internet network.
본 발명에 있어서, 바람직하게는 저장 장치 혹은 RF IC는 디스크에 관한 사용 이력 사항 및 분석 결과를 보관하는 것을 특징으로 한다.In the present invention, preferably, the storage device or the RF IC stores the usage history and the analysis result of the disk.
본 발명에 있어서, 상기 중앙 제어 장치는 상기 저장 장치 혹은 RF IC에 저장된 정보를 독출하여 상기 입출력 장치를 통해 원격으로 서버에 전송하는 것을 특징으로 한다.In the present invention, the central control unit is characterized in that the information stored in the storage device or RF IC to read and transmit to the server remotely through the input and output device.
본 발명에 있어서, 통상의 광학디스크(예를 들면, CD, DVD) 혹은 인식 불가한 미생물 분석 장치에 사용 불가한 디스크 로딩시 자동 추출(eject)하든지 경고 메시지를 사용자에게 보내는 것을 특징으로 한다.In the present invention, a warning message is sent to the user, whether to be automatically ejected when loading a disc which is not usable in a conventional optical disc (for example, CD, DVD) or an unrecognized microbial analysis device.
또한, 본 발명은 (a)샘플러를 이용해 프렙 챔버에 검체를 주입하는 단계; (b) 상기 프렙 챔버 내의 검체를 배지 챔버로 이송하는 단계; (c) 배지 챔버로 이송된 검체를 가지고 배지 챔버 내의 배지 표면에 도포 접종하는 단계; (e) 도포 접종 후 잉여 검체를 트레쉬 챔버에 모으는 세정 단계를 포함하는 본 발명에 따른 미생물 분석장치를 이용한 분석 방법을 제공한다.In addition, the present invention (a) injecting a sample to the prep chamber using a sampler; (b) transferring the sample in the preparation chamber to the media chamber; (c) applying and inoculating the medium surface in the medium chamber with the sample transferred to the medium chamber; (e) It provides an analysis method using a microbial analysis apparatus according to the present invention comprising a washing step of collecting the surplus specimen in the trech chamber after application inoculation.
상기 분석방법은 증균 배양단계를 더 포함할 수 있다.The analysis method may further comprise a enrichment culture step.
상기 분석방법은 이미지 센서에 의한 배지 챔버의 판독 단계를 더 포함할 수 있다.The analysis method may further include reading the medium chamber by the image sensor.
상기 분석방법은 배지 챔버 판독 단계 및 미생물 집락의 계수화 단계를 더 포함할 수 있다.The analysis method may further comprise a medium chamber reading step and the microbial colonization step.
상기 분석방법은 배지 챔버 판독 결과를 원격지 서버로 송신하는 단계를 더 포함할 수 있다.The analysis method may further include transmitting the medium chamber reading result to a remote server.
이러한 목적을 달성하기 위해 본 발명은 검체를 주입하기 위한 검체 주입구; 검사부위로부터 검체를 수집하고 수집된 검체를 상기 검체 주입구에 주입하기 위한 샘플러(sampler); 상기 검체 주입구로부터 주입된 검체를 일시 저장하기 위한 프렙 챔버; 상기 프렙 챔버로 부터의 검체를 취해, 검체 내에 존재하는 미생물을 증균하기 위한 액체 배양액(liquid culture medium)를 포함하는 증균 챔버; 상기 증균된 미생물에 대해 영양분을 제공하기 위한 배지(culture medium)를 제공하는 적어도 하나 이상의 배지 챔버; 찌꺼기 및 과잉 검체를 모으기 위한 트레쉬 챔버; 및 상기 챔버들 사이에 유체 및 검체를 이동시키기 위한 밸브; 상기 검체의 이동 경로를 제공하는 유로(채널); 및 상기 유로, 밸브 및 챔버가 집적화된 회전 가능한 디스크형 몸체를 구비한 미생물 분석 장치 및 이를 이용한 분석 방법을 제공한다.In order to achieve the above object, the present invention provides a sample injection port for injecting a sample; A sampler for collecting a sample from an inspection site and injecting the collected sample into the sample inlet; A prep chamber for temporarily storing a sample injected from the sample inlet; An enrichment chamber containing a sample from the prep chamber and containing a liquid culture medium for enriching the microorganisms present in the sample; At least one medium chamber providing a culture medium for providing nutrients to the enriched microorganisms; A trech chamber for collecting debris and excess sample; And a valve for moving fluid and sample between the chambers; A flow path (channel) for providing a movement path of the specimen; And it provides a microorganism analysis device having a rotatable disk-shaped body in which the flow path, valve and chamber are integrated and an analysis method using the same.
본 발명의 미생물 분석 장치에 있어서, 상기 몸체는 플라스틱, 유리, 운모, 실리카, 실리콘 웨이퍼 등의 다양한 재료로부터 선택될 수 있다. 그러나, 플라스틱이 경제적 이유, 가공의 용이성, CD-ROM 및 DVD 판독기와 같은 기존의 레이저 반사 기초 탐지기와의 양립성 때문에 선호된다. 사용가능한 플라스틱으로는 폴리프로필렌, 폴리아크릴레이트, 폴리비닐알콜, 폴리에틸렌, 폴리메틸메타크릴레이트(PMMA: polymethyl methacrylate), COC(고리형 올레핀 고분자: Cyclic Olefin Copolymer), 아크릴 및 폴리카보네이트가 있다. 이중 폴리프로필렌, COC, 아크릴 과 폴리카보네이트가 선호되며 아크릴, COC와 폴리카보네이트가 가장 선호된다. In the microbial analysis apparatus of the present invention, the body may be selected from various materials such as plastic, glass, mica, silica, silicon wafer, and the like. However, plastics are preferred for economic reasons, ease of processing, and compatibility with existing laser reflection based detectors such as CD-ROM and DVD readers. Plastics that can be used include polypropylene, polyacrylates, polyvinyl alcohol, polyethylene, polymethyl methacrylate (PMMA), Cyclic Olefin Copolymer (COC), acrylics and polycarbonates. Of these, polypropylene, COC, acrylic and polycarbonate are preferred, and acrylic, COC and polycarbonate are most preferred.
또한 상기 몸체의 표면은 챔버 내에 저장된 액체의 증발을 막기 위해 알루미늄 코팅될 수 있다.The surface of the body may also be aluminum coated to prevent evaporation of the liquid stored in the chamber.
본 발명의 미생물 분석 장치에 있어서, 상기 프렙 챔버, 증균 챔버, 배지 챔버, 트레쉬 챔버; 및 상기 챔버들간의 유체이동을 위한 유로, 유공 및 밸브가 디스크형 몸체에 형성 배치되어 미생물 분석에 필요한 제반 공정을 구성하는 것을 특징으로 하며, 또한 상기 디스크형 몸체는 상부 몸체와 하부 몸체가 적층되어 구성된 것을 특징으로 한다. In the microbial analysis apparatus of the present invention, the prep chamber, enrichment chamber, medium chamber, trech chamber; And a flow path, a hole, and a valve for fluid movement between the chambers are formed in the disc-shaped body to constitute various processes required for microbial analysis, and the disc-shaped body is formed by stacking an upper body and a lower body. Characterized in that configured.
상기 커버 수단은 상기 상부 몸체와 하부 몸체간에 인력을 형성하는 자력에 의해 닫히고, 실험자가 동정(identification)실험을 위해, 상기 커버 수단의 개방을 통해 배지 챔버로부터 의심되는 군락의 샘플을 채집하는 것을 특징으로 한다.The cover means is closed by a magnetic force forming an attractive force between the upper body and the lower body, and the experimenter collects a sample of the suspected colony from the medium chamber through the opening of the cover means for identification experiments. It is done.
본 발명의 또 다른 측면은 상기 커버 수단은 상기 상부 몸체내에는 영구 자석이 설치되고 하부 몸체내에 강자성체가 설치되어 상부 몸체와 하부 몸체간에 인력에 의해 닫히는 것이 선호된다.In another aspect of the present invention, the cover means is preferably a permanent magnet is installed in the upper body and a ferromagnetic material is installed in the lower body is closed by the attraction between the upper body and the lower body.
본 발명의 또 다른 측면은 상기 커버 수단은 개방수단을 더 구비하여, 개방수단에 의해 손쉬운 개방이 허여되는 것을 특징으로 한다.Another aspect of the present invention is characterized in that the cover means further comprises an opening means, allowing easy opening by the opening means.
상기 개방수단은 상기 상부 몸체 내의 영구 자석에 대해 척력을 발생시키는 전자석이 내장된 전자석 보드(board)가 선호된다. 전자석 보드 온(ON)시 상기 하부 몸체 내의 영구 자석에 대해 척력을 발생시켜, 몸체로부터 하부 몸체의 손쉬운 분리가 가능하다.The opening means is preferably an electromagnet board in which an electromagnet is generated which generates a repulsive force on the permanent magnet in the upper body. When the electromagnet board on (ON) generates a repulsive force for the permanent magnet in the lower body, it is possible to easily separate the lower body from the body.
상기 상부 몸체와 하부 몸체는 초음파 융착(Ultrasonic welding) 혹은 레이저 융착, 박막 접착 테이프에 의해 결합될 수 있다. 이러한 레이저는 광 투과율이 다른 물질간의 경계면에서 열이 잘 발생하므로, 레이저 융착을 위해, 상기 상부 몸체와 하부 몸체는 서로 다른 종류의 상기의 플라스틱 재료를 사용하는 것이 선호된다. The upper body and the lower body may be combined by ultrasonic welding or laser welding, a thin film adhesive tape. Since such lasers generate heat well at the interface between materials having different light transmittances, it is preferable that the upper body and the lower body use different kinds of plastic materials for laser welding.
본 발명의 미생물 분석 장치에 있어서, 상기 박막 접착 테이프는 양면테입 등 모든 접착테이프에 사용되는 점착제(an adhesive;a gluing agent)가 선호되며, 점착제는 실리콘, 고무계, 변성실리콘계, 아크릴계(acrylic), 폴리에스터, 에폭시등과 같은 재료가 사용될 수 있다. In the microbial analysis apparatus of the present invention, the thin adhesive tape is preferably an adhesive (a gluing agent) used for all adhesive tapes such as double-sided tape, and the adhesive is silicone, rubber, modified silicone, acrylic, Materials such as polyester, epoxy and the like can be used.
일반적으로, 상기 양면 테입은 종이(paper), 비닐, 폴리에스터 필름(polyester film), Polyethylene film 및 기타 합성 재질 같은 이형지의 양쪽 혹은 한쪽면에 특수한 점착제(an adhesive;a gluing agent)로 표면 처리가 되어 있고, 필요로 하는 조건에 따라 높은 실링 및 완충, 진동완화, 내충격성, 내열성, 흡착성, 접착력 등의 특징을 가진 점착제 재료를 선정하여 사용할 수 있다. In general, the double-sided tape is surface treated with an adhesive (a adhesive) on both or one side of the release paper such as paper, vinyl, polyester film, polyethylene film and other synthetic materials. According to the required conditions, it is possible to select and use a pressure-sensitive adhesive material having characteristics such as high sealing and buffering, vibration relaxation, impact resistance, heat resistance, adsorption, and adhesive strength.
상기 상부 몸체와 하부 몸체중 한쪽의 접착면에 양면 테이프를 붙인후 이형지를 제거함으로써 몸체의 한쪽면에 점착제에 의한 박막 코팅을 하든지 점착제를 디스펜서(dispenser) 혹은 스프레이(spray) 혹은 실크 스크린 인쇄하여 몸체의 한쪽면을 점착제에 의한 박막 코팅을 하는 것이 선호된다. By attaching a double-sided tape on one side of the upper body and the lower body and then removing the release paper, a thin film coating by adhesive on one side of the body or by applying a dispenser or spray or silk screen printing on the adhesive It is preferable to apply a thin film coating on one side of the adhesive.
본 발명의 미생물 분석 장치에 있어서, 상기 점착제에 의해 박막 코팅된 상부 몸체와 하부 몸체를 서로 맞대어 밀착 부착하여 하나의 미생물 분석장치의 몸체를 조립하는 것을 특징으로 한다. In the microorganism analyzing apparatus of the present invention, the body of one microorganism analyzing apparatus is assembled by attaching a thin film-coated upper body and a lower body to each other in close contact with each other.
상기 양면 테이프 "수압 버스트 밸브"의 유공폐쇄막이 포함되어 있어, 이형지 제거시 유공부위에 점착제에 의한 유공폐쇄막을 남기거나 상기 박막 코팅 동안 "수압 버스트 밸브"를 위한 유공폐쇄막을 형성하는 것이 선호된다.A pore closure membrane of the double-sided tape "hydraulic burst valve" is included, so that it is preferred to leave a pore-closing membrane with an adhesive on the pore area when removing the release paper or to form a pore-closing membrane for the "hydraulic burst valve" during the thin film coating.
본 발명의 미생물 분석 장치에 있어서, 상기의 유공폐쇄막은 박막 접착 테이프 대신에 유압 혹은 원심력에 의해 쉽게 찢어질 수 있는 막(membrane)이 사용될 수 있으며, 막 표면은 물과 친화성 없는 소수성(hydrophobic) 막으로 PE(Polyethylene), PP(Polypropylene), PS(Polysulfone), Polyalkene, Cellulosics, Polyvinyl, Polycarbonate, Polyamide와 같은 폴리머 막이 사용될 수 있다. 소수성 막 표면은 챔버에 저장된 유체의 이동을 효과적으로 차단한다.In the microbial analysis apparatus of the present invention, the porous closed membrane may be a membrane that can be easily torn by hydraulic or centrifugal force instead of a thin film adhesive tape, and the surface of the membrane is hydrophobic without affinity with water. Polymer membranes such as polyethylene (PE), polypropylene (PP), polysulfone (PS), polyalkene, cellulosics, polyvinyl, polycarbonate, and polyamide may be used as the membrane. The hydrophobic membrane surface effectively blocks the movement of fluid stored in the chamber.
본 발명의 미생물 분석 장치에 있어서, 상기 마개는 원기둥, 혹은 구슬(ball) 혹은 박막 원기둥 중 선택된 모양으로 이루어지는 것을 특징으로 한다. 상기 마개는 점착제가 코팅된 구슬이거나 왁스가 선호된다. In the microbial analysis device of the present invention, the stopper is characterized in that the cylinder, or a ball (ball) or a thin film selected from the shape of the cylinder. The stopper is a pressure-sensitive adhesive coated wax or a wax is preferred.
상기 왁스는 파라핀 왁스(paraffin wax), 합성 왁스(synthetic wax), 마이크로 크리스탈린 왁스(microcrystalline wax)가 선호된다.The wax is preferably paraffin wax, synthetic wax, or microcrystalline wax.
본 발명에 있어서, 상기 마개 혹은 마개 표면에 코팅된 점착제는 조사된(irradiated) 레이저 빔의 열에 의해 접착력이 약해지든지 녹는 것이 선호된다.In the present invention, the adhesive or the adhesive coated on the surface of the plug is preferably melted or weakened by the heat of the irradiated laser beam.
상기 점착제(an adhesive)은 조사된(irradiated) 레이저 빔의 열에 의해 버스트 밸브의 접착강도가 약해지고 디스크 회전에 의해 버스트 밸브가 개방된다.The adhesive has a weak adhesive strength of the burst valve due to the heat of the irradiated laser beam, and the burst valve is opened by the disk rotation.
본 발명의 미생물 분석 장치 및 이를 이용한 분석방법은 유체 내 소량의 미생물을 탐지하는데 적합하다. 특히 본 발명에 따른 미생물 분석 장치 및 이를 이용한 분석 방법은 미생물의 증균, 도포(접종), 배양, 검출 등의 일련의 공정을 자동화할 수 있다는 효과를 제공한다. Microbial analysis device and an analysis method using the same of the present invention is suitable for detecting a small amount of microorganisms in the fluid. In particular, the microbial analysis apparatus according to the present invention and an analysis method using the same provide an effect of automating a series of processes such as microbial enrichment, application (inoculation), culture, and detection.
도 1 내지 도 4는 본 발명의 일 실시예에 따른 미생물 분석 장치에서 사용되는 버스트 밸브의 다양한 실시예를 도시한 도면이며, 1 to 4 is a view showing various embodiments of a burst valve used in the microbial analysis apparatus according to an embodiment of the present invention,
도 5 내지 도 7은 "자석 버스트 밸브"의 단면도 및 디스크속에 매립된 자석밸브를 사용한 "자석 버스트 밸브"의 다양한 실시예를 도시한 도면이며,5 to 7 illustrate various embodiments of a “magnetic burst valve” using a cross sectional view of the “magnetic burst valve” and a magnet valve embedded in a disc;
도 8 내지 도 10은 본 발명의 일 실시예에 따른 미생물 분석 장치를 개략적으로 도시한 도면이며, 8 to 10 is a view schematically showing a microbial analysis apparatus according to an embodiment of the present invention,
도 11은 턴테이블과 압착수단에 대한 다른 일 실시예이며,11 is another embodiment of a turntable and a crimping means,
도 12는 미생물 분석을 위한 제반 공정이 집적화된 디스크의 실시예이며,12 is an embodiment of a disk incorporating various processes for analyzing microorganisms,
도 13은 "수압 버스트 밸브"의 상세도이며,13 is a detailed view of a "hydraulic burst valve",
도 14는 본 발명의 일 실시예에 따른 미생물 분석 장치에서 사용되는 샘플러를 도시한 도면이며,14 is a view showing a sampler used in the microbial analysis apparatus according to an embodiment of the present invention,
도 15는 배지 챔버 내에서 미생물을 배양된 결과를 보여주는 도면이며, 15 is a view showing the results of culturing microorganisms in the medium chamber,
도 16은 항온기 또는 온도제어장치를 내장한 탑로딩(top loading) 방식의 미생물 분석 장치의 일실시예를 도시하는 도면이며, FIG. 16 is a diagram illustrating an embodiment of a top loading microorganism analysis device incorporating a thermostat or a temperature control device.
도 17은 항온기 또는 온도제어장치를 트레이 내부에 내장한 프런트 로딩(front loading) 방식의 미생물 분석 장치의 일실시예를 도시하는 도면이며, FIG. 17 is a view showing an embodiment of a front loading type microbial analysis apparatus in which a thermostat or a temperature control device is built into a tray.
도 18은 커버 수단이 몸체의 최외주와 최내주에 설치된 일실시예를 도시하는 도면이며, 18 is a view showing an embodiment in which the cover means is installed on the outermost and innermost circumference of the body,
도 19는 전자석 보드를 사용한 개방 수단의 일 실시예를 도시하는 도면이며,19 is a view showing an embodiment of an opening means using an electromagnet board,
도 20은 배지 챔버 내의 호기성 미생물에 산소를 공급하기 위한 산소 공급용 밸브의 일실시예를 도시하는 도면이며, 20 is a view showing an embodiment of an oxygen supply valve for supplying oxygen to the aerobic microorganisms in the medium chamber,
도 21은 테스트 스트립(test strip)을 사용한 미생물분석장치의 일실시예를 도시하는 도면이다. FIG. 21 is a diagram illustrating an embodiment of a microbial analysis apparatus using a test strip. FIG.
이하, 본 발명에 따른 미생물 분석 장치 및 이를 이용한 분석 방법의 바람직한 실시예를 첨부된 도면을 참조하여 설명한다. 이 과정에서 도면에 도시된 선들의 두께나 구성요소의 크기 등은 설명의 명료성과 편의상 과장되게 도시되어 있을 수 있다. 또한, 후술되는 용어들은 본 발명에서의 기능을 고려하여 정의된 용어들로서 이는 사용자, 운용자의 의도 또는 관례에 따라 달라질 수 있다. 그러므로 이러한 용어들에 대한 정의는 본 명세서 전반에 걸친 내용을 토대로 내려져야 할 것이다. Hereinafter, with reference to the accompanying drawings a preferred embodiment of the microbial analysis apparatus and an analysis method using the same according to the present invention will be described. In this process, the thickness of the lines or the size of the components shown in the drawings may be exaggerated for clarity and convenience of description. In addition, terms to be described below are terms defined in consideration of functions in the present invention, which may vary according to the intention or convention of a user or an operator. Therefore, the definitions of these terms should be made based on the contents throughout the specification.
<실시예> <Example>
도 1 내지 도 4는 본 발명의 일 실시예에 따른 미생물 분석장치에서 사용되는 버스트 밸브의 다양한 실시예를 나타낸다. 1 to 4 illustrate various embodiments of a burst valve used in a microbial analysis apparatus according to an embodiment of the present invention.
도 1 및 도 2는 미생물 분석장치의 디스크 또는 몸체 속에 설치된 박막 접착 테이프를 사용한 수압 버스트 밸브의 a-b사이의 단면도 및 분해도이다. 특히, 도 2는 유공(10b)이 몸체(100)의 회전에 의해 액체(도시 안됨) 자체에 발생된 유압에 의해 유공폐쇄막(13a)이 뜯겨져 유공(10b)이 열려 챔버(11a)내에 저장된 액체가 챔버(11b)로 이동된 것을 나타낸다.1 and 2 are cross-sectional views and exploded views of a-b of the hydraulic burst valve using a thin film adhesive tape installed in the disk or body of the microbial analyzer. In particular, FIG. 2 shows that the hole closing film 13a is torn off by the hydraulic pressure generated in the liquid (not shown) by the rotation of the body 100 so that the hole 10b is opened in the chamber 11a. It indicates that the stored liquid has been moved to the chamber 11b.
몸체(100)는 상부 몸체(1)와 하부 몸체(2)로 구성되며, 이들 각각에는 사출 성형 공정 동안 몸체 표면에 유체가 흐를 수 있는 유로(16a); 검체나 액체 배양액 같은 용액 등을 저장할 수 있는 챔버(11a, 11b); 및 상기 챔버(11a, 11b)를 연결시키는 유공(10b)이 형성된다. 이러한 상부 및 하부 몸체(1, 2)는 박막 접착 테이프(2a)에 의해 서로 밀착 부착되어 하나의 몸체(100)를 이룬다.The body 100 includes an upper body 1 and a lower body 2, each of which includes a flow path 16a through which fluid can flow on the body surface during the injection molding process; Chambers 11a and 11b capable of storing a sample or a solution such as a liquid culture solution; And a hole 10b connecting the chambers 11a and 11b. The upper and lower bodies 1 and 2 are closely attached to each other by the thin film adhesive tape 2a to form one body 100.
구체적으로 상기 상부 몸체(1)와 하부 몸체(2)에는 상기 챔버들(11a, 11b)를 구성하고 하부 몸체(2)에는 상기 챔버(11a)와 챔버(11b)를 연결시키기 위한 유로(16a)가 일정 깊이로 음각되어 있고, 상기 유로(16a)의 말단부에는 상기 챔버(11a)와 챔버(11b)간의 연결을 제공하는 유공(10b)이 형성된다. 이러한 유공(10b)은 유공폐쇄막(13a)에 의해 폐쇄된다. 유공폐쇄막(13a)은 상부 몸체(1)와 하부 몸체(2)의 부착 조립시 박막 접착 테이프(2a)에 의해 유공(10b) 부위에 형성된다.Specifically, the upper body 1 and the lower body 2 constitute the chambers 11a and 11b, and the lower body 2 has a flow path 16a for connecting the chamber 11a and the chamber 11b. Is engraved to a certain depth, and a hole 10b is formed at the distal end of the flow path 16a to provide a connection between the chamber 11a and the chamber 11b. This hole 10b is closed by the hole blocking membrane 13a. The hole closing membrane 13a is formed at the portion of the hole 10b by the thin film adhesive tape 2a when the upper body 1 and the lower body 2 are attached and assembled.
상기 유공폐쇄막(13a)에 의해 유공(10b)을 폐쇄함으로써 유통보관 기간 동안 유공(10b)을 완전히 차단하고, 사용시 몸체(100)의 고속회전에 의한 원심력에 의해 챔버(11a) 내에 저장된 유체 자체에 형성된 유압에 의해 유공폐쇄막(13a)이 뜯겨져 나가 유공(10b)이 개방되어 유체가 챔버(11b)로 이동하게 된다. 상기 유공폐쇄막(13b)은 프렉셔블(flexible)하므로 온도와 같은 환경적 요소에 따른 팽창과 수축에 잘 적응하기 때문에 유통보관 기간 중에 액체의 증발 혹은 몸체의 팽창 및 수축에 의한 실링(sealing) 문제가 발생하지 않는다는 장점이 있다.Closing the pores 10b by the hole closing membrane 13a completely blocks the pores 10b during the distribution storage period, and the fluid itself stored in the chamber 11a by centrifugal force by the high speed rotation of the body 100 during use. The hole closed membrane 13a is torn off by the hydraulic pressure formed in the hole, and the hole 10b is opened to move the fluid into the chamber 11b. Since the pore-closing membrane 13b is flexible, it adapts well to expansion and contraction according to environmental factors such as temperature, and thus sealing problems due to evaporation of liquid or expansion and contraction of the body during distribution. There is an advantage that does not occur.
본 발명에 있어서, 상기 유공폐쇄막(13b)은 상기 상부 몸체(1)와 하부 몸체(2)가 박막 접착 테이프(2a)에 의해 서로 밀착하여 부착 조립될 때 유공(10b)부위에 형성되는 것을 특징으로 한다. In the present invention, the pore-closing membrane 13b is formed in the hole 10b when the upper body 1 and the lower body 2 are attached and assembled in close contact with each other by a thin film adhesive tape 2a. It features.
본 발명의 다른 측면은, 상기 박막 접착 테이프(2a)에 의한 유공 폐쇄시 폐쇄강도(closing strength)는 유공폐쇄막(13a)과 하부 몸체(2)와의 접착 면적(14)에 비례하므로, 상기 몸체(100) 내에 복수의 "수압 버스트 밸브"를 설치하고 이들의 폐쇄 강도(closing strength)를 서로 달리하여 원하는 시점에서 원하는 밸브를 개방키 위해 해당밸브의 유공폐쇄막(13b)의 폐쇄강도 이상의 유압(hydraulic force, hydraulic pressure)이 발생하도록 하는 원심력을 발생시켜 선택적 또는 개별적으로 유공(10b)이 개방되도록 하는 것을 특징으로 한다.According to another aspect of the present invention, the closing strength when closing the pores by the thin film adhesive tape 2a is proportional to the adhesion area 14 between the pore-closing membrane 13a and the lower body 2, so that the body A plurality of "hydraulic burst valves" are installed in the 100 and their closing strengths are different from each other so as to open a desired valve at a desired point in time so as to provide a hydraulic pressure greater than the closing strength of the closed hole membrane 13b of the corresponding valve. Hydraulic force, hydraulic pressure) to generate a centrifugal force is characterized in that the hole (10b) to be opened selectively or individually.
또한 밸브 개방을 원할시, 레이저 빔을 사용하여 해당 유공폐쇄막(13b) 부위에 열을 가함으로써 "수압 버스트 밸브"의 접착강도가 약해지고 디스크 회전에 의해 수압 버스트 밸브가 쉽게 개방된다.In addition, when the valve is to be opened, the adhesive strength of the "hydraulic burst valve" is weakened by applying heat to the hole-closing membrane 13b using a laser beam, and the hydraulic burst valve is easily opened by disk rotation.
본 발명의 또 다른 측면은, 상기 유공폐쇄막(13a)은 상변화물질(Phase Change material)에 의해 박막 접착 테이프 층을 구성하는 것을 특징으로 한다. 이때, 상기 상변화 물질은 40도~70도의 용융점(melting point)을 갖는 것이 바람직하다. In another aspect of the present invention, the pore-closing membrane 13a is characterized by constituting a thin film adhesive tape layer by a phase change material. In this case, the phase change material preferably has a melting point of 40 degrees to 70 degrees.
본 발명의 또 다른 측면은, 상기 유공폐쇄막(13a)은 열 가소성 점착제 재료, 그리고 나머지 접합부분은 열 경화성 점착제 재료에 의해 박막 접착 테이프 층을 구성하는 것을 특징으로 한다. Another aspect of the present invention is characterized in that the pore-closing membrane 13a is made of a thermoplastic adhesive material, and the remaining bonding portion constitutes a thin film adhesive tape layer by a thermosetting adhesive material.
일 실시예로 상기 유공폐쇄막(13a)은 열 가소성인 핫 멜트 점착제(hot malt adhesive)에 의해 형성된 박막 잡착 테이프이고 나머지 접합부는 열 경화성인 아크릴계 점착제에 의해 형성된 박막 접착 테이프를 사용할 수 있다.In one embodiment, the pore-closing film 13a may be a thin film adhesive tape formed by a thermoplastic hot malt adhesive, and the remaining joint may be a thin film adhesive tape formed by an acrylic adhesive which is thermosetting.
이 경우, 레이저 빔의 열에 의해 나머지 부위보다 유공폐쇄막의 접착력이 쉽게 약화된다. 이 경우 "스캔닝 빔" 동작에 의해 버스트 밸브를 개방하는 것이 선호된다. 즉, 유공폐쇄막(13a)은 열 가소성 점착제이고 나머지 부분은 열 경화성 점착제 재료에 의해 박막 접착 테이프 층을 구성되므로 "스캐닝 빔" 동작 동안 열 경화성 테이프의 연화점은 열 가소성 테이프의 연화점보다 훨씬 높기 때문에 열 경화성 테이프 부분의 접합은 가열 여부에 관계없이 접합이 잘 유지된다. In this case, the adhesive force of the pore-closing membrane is weakened more easily by the heat of the laser beam than in the remaining portions. In this case it is preferred to open the burst valve by a "scanning beam" operation. That is, since the pore-closing film 13a is a thermoplastic adhesive and the remainder is composed of a thin film adhesive tape layer by the thermosetting adhesive material, the softening point of the thermosetting tape during the "scanning beam" operation is much higher than the softening point of the thermoplastic tape. Bonding of the thermosetting tape portion is well maintained with or without heating.
유공폐쇄막(13a)은 레이점 빔의 열에 의해 쉽게 연화되어 접착력이 약해지는 반면 그 주위 및 나머지 부분은 열 경화성 테이프에 의해 접착이 되어 있으므로 레이저 빔의 열에 의해 접착력이 약화되지 않는 장점을 제공한다. The pore-closing film 13a is easily softened by the heat of the ray point beam, and thus weakens the adhesive force, while the surrounding and the remaining parts are bonded by the heat-curable tape, thereby providing the advantage that the adhesive force is not weakened by the heat of the laser beam. .
상기 유공폐쇄막(13a)을 구성하는 점착제의 연화점은 나머지 접합부를 구성하는 점착제의 연화점보다 낮은 것이 선호되면, 상기 열 경화성 점착제의 연화점(Softening Temperature) 은 120도 이상인 것이 선호되며, 열 가소성 점착제의 연화점은 60도에서 80도 사이인 것이 더욱 선호된다.If the softening point of the pressure-sensitive adhesive constituting the pore-closing membrane 13a is lower than the softening point of the pressure-sensitive adhesive constituting the remaining joints, the softening temperature of the thermosetting pressure-sensitive adhesive (Softening Temperature) is preferably 120 degrees or more, and the thermoplastic pressure-sensitive adhesive The softening point is more preferably between 60 and 80 degrees.
상기 열가소성수지는, COC, PMMA, PC, PS, POM, PFA, PVC, PP, PET, PEEK, PA, PSU 및 PVDF가 선호된다.The thermoplastic resin is preferably COC, PMMA, PC, PS, POM, PFA, PVC, PP, PET, PEEK, PA, PSU and PVDF.
도 3 및 도 4는 몸체(100)속에 매립된 마개를 사용한 "마개 버스트 밸브"의 일례이다.3 and 4 show an example of a “bung burst valve” using a plug embedded in the body 100.
상기 몸체(100)는 상부 몸체(1)와 하부 몸체(2)로 구성되며, 이들 각각은 사출 성형 공정 동안 몸체 표면에 유체가 흐를 수 있는 유로(16a); 용액을 저장할 수 있는 챔버(11a, 11b); 그리고 상기 챔버(11a, 11b)를 연결시키는 유공(10b)을 형성한다. 상기 유공(10b)은 유공부위에 매립된 마개(13b)에 의해 폐쇄되며, 마개(13b)에 의해 유공을 폐쇄함으로써 유통보관 기간 동안 유공을 완전히 차단하고, 사용시 몸체(100)의 고속회전에 의한 원심력이 유로(16a)와 마개 간에 형성된 결합력보다 강한 시점에서 상기 마개(13b)가 보조채널(16b) 쪽으로 이탈하여 유공(10b)이 개방된다.The body 100 is composed of an upper body (1) and the lower body (2), each of which has a flow path (16a) through which fluid can flow on the body surface during the injection molding process; Chambers 11a and 11b capable of storing solutions; And the hole (10b) for connecting the chamber (11a, 11b) is formed. The hole (10b) is closed by a stopper (13b) buried in the hole, by closing the hole by the stopper (13b) to completely block the hole during the distribution storage period, by the high-speed rotation of the body 100 in use When the centrifugal force is stronger than the coupling force formed between the flow path 16a and the stopper, the stopper 13b is released toward the auxiliary channel 16b so that the hole 10b is opened.
또한 본 발명의 다른 측면은 상기 마개(13b)에 의한 유공(10b) 폐쇄시 폐쇄강도(closing strength)는 마개와 유로(16a)간의 접촉 면적에 비례하므로, 상기 몸체(100)에 복수의 "마개 버스트 밸브"를 설치하고 이들의 폐쇄 강도(closing strength)을 서로 달리하여 원하는 시점에서 원하는 밸브를 개방하기 위해 해당 밸브의 폐쇄강도 이상의 원심력이 발생하도록 디스크를 회전시켜 유공(10b)이 선택적 또는 독립적으로 개방되도록 하는 것을 특징으로 한다.In addition, another aspect of the present invention is because the closing strength (closing strength) when closing the hole (10b) by the stopper (13b) is proportional to the contact area between the stopper and the flow path (16a), a plurality of "stopper" in the body 100 Burst valves "and their closing strengths are different from each other so that the perforations 10b can be selectively or independently by rotating the disc to generate a centrifugal force above the closing strength of that valve to open the desired valve at a desired point in time. It is characterized in that the opening.
상기 마개의 지름은 1mm 내지 5mm가 바람직하다. 상기 지름의 크기가 커지면 접촉면이 증가하여 개폐에 대한 신뢰도가 증가하게 된다. 또한, 본 발명에서는 상기 마개는 구형일수도 있으나 박막 입자도 사용될 수 있다. 본 발명에서는 박막 입자로서 박막형 원기둥 혹은 박막형 사각형이 선호된다. 상기 박막 입자의 두께는 약 0.1mm 내지 2mm가 선호된다. The diameter of the stopper is preferably 1mm to 5mm. As the diameter increases, the contact surface increases, thereby increasing the reliability of opening and closing. In addition, in the present invention, the plug may be spherical but thin film particles may also be used. In the present invention, a thin film cylinder or a thin film square is preferred as the thin film particles. The thickness of the thin film particles is preferably about 0.1 mm to 2 mm.
도 4의 왼쪽 그림은 상기 마개(13b)에 의해 유공(10b)이 막혀 유로(16a)가 차단된 경우를 나타내고, 도 4의 오른쪽 그림은 유공(10b)이 몸체(100) 회전에 의해 발생된 원심력에 의해 마개(13b)를 유공으로부터 이탈시켜 보조 채널(16b) 쪽으로 이동시킴으로써 유공(10b)이 열린 경우를 나타낸다. 4 shows a case in which the oil hole 10b is blocked by the stopper 13b and the flow path 16a is blocked. In the right picture of FIG. 4, the oil hole 10b is generated by the rotation of the body 100. The case where the hole 10b is opened by moving the stopper 13b out of the hole by the centrifugal force and moving toward the auxiliary channel 16b.
도 5 내지 도 7은 "자석 버스트 밸브"의 a-b사이의 단면도 및 몸체(100)속에 매립된 자석밸브를 사용한 "자석 버스트 밸브"의 여러 실시예이다.5-7 are various embodiments of a "magnetic burst valve" using a cross sectional view between a-b of the "magnetic burst valve" and a magnet valve embedded in the body 100.
도면 부호 90은 영구자석으로 구성된 자석밸브이고 이러한 자석밸브는 자성체로 구성된 구속홈(101)과 인력에 의해 유공(10b)을 닫는다. 상기 몸체(100)의 회전시 자석밸브(90)는 원심력에 의해 구속홈(101)으로부터 이탈되어 유공(10b)이 개방된다. 또한 이탈된 자석밸브(90)가 어느 범위이상 벗어나지 않도록 경계막(91)이 설치된다. Reference numeral 90 denotes a magnet valve composed of permanent magnets, and the magnet valve closes the oil hole 10b by the attraction groove and the attraction force 101 formed of the magnetic material. When the body 100 rotates, the magnetic valve 90 is separated from the restraint groove 101 by the centrifugal force to open the oil hole 10b. In addition, the boundary membrane 91 is installed so that the detached magnet valve 90 does not deviate more than a certain range.
상기 구속홈(101)은 몸체(100)의 흔들림에 의해 상기 자석 밸브(90)가 떨어져 나가는 것을 방지하는 역할을 수행한다. 본 발명에서 선호되는 구속홈(101)의 지름은 자석밸브(90)의 지름보다 20% 내지 70%가 더 큰 지름이 선호된다.The restraint groove 101 serves to prevent the magnetic valve 90 from falling off by shaking the body 100. In the present invention, the diameter of the constrained groove 101 is preferably 20% to 70% larger than the diameter of the magnet valve 90.
디스크 정지시, 상기 이탈되었던 자석밸브(90)는 구속홈(101)과의 인력에 의해 유공을 다시 밀폐시킨다. 상기 자석 밸브(90)는 영구자석으로 형성될 수 있으며 유공의 밀폐력을 증가시키기 위해 그 위에 실리콘 고무(silicone rubber) 같은 고무 쿠션(Cushion)재료의 코팅이 이루어질 수 있다. 박막 원형 자석, 박막 원기둥 자석 혹은 박막 사각형 자석 혹은 구형 자석(ball magnet)이 선호된다.When the disc stops, the detached magnet valve 90 again seals the hole by the attraction force with the restraint groove 101. The magnetic valve 90 may be formed of a permanent magnet and may be coated with a rubber cushion material such as silicone rubber thereon to increase the sealing force of the pores. Thin film circular magnets, thin film cylindrical magnets or thin film square magnets or ball magnets are preferred.
도 6의 윗 그림은 원심력에 의해 유공(10b)이 열린 경우를 나타내고 아래 그림은 유공(10b)이 닫힌 경우를 나타낸다.The upper figure of FIG. 6 shows the case where the hole 10b is opened by centrifugal force, and the figure below shows the case where the hole 10b is closed.
도 7은 상기 자석 밸브(90)의 밀폐력을 향상시키기 위해 강자성체(92b)의 표면 위에 실리콘 고무(silicone rubber) 같은 고무 쿠션(Cushion)재료(92a)가 코팅된 강자성체 링(92)(ring)에 의해 상기 구속홈(101)에 삽입 조립한 일실시예이다. 이 경우 몸체(1,2)는 강자성체가 아니어도 되는 장점이 있다.7 shows a ferromagnetic ring 92 coated with a rubber cushion material 92a such as silicone rubber on the surface of the ferromagnetic material 92b to improve the sealing force of the magnetic valve 90. By inserting into the restraint groove 101 is an embodiment. In this case, the body (1, 2) has the advantage that it does not have to be a ferromagnetic material.
도 8 내지 도 10은 본 발명의 미생물 분석 장치(200)의 일실시예로 미생물 분석을 위한 제반 공정이 집적화된 디스크(100)와 주변 구동 장치의 일실시예를 보이며, 이에 대한 평면도 및 단면도이다.8 to 10 illustrate an embodiment of a disk 100 and peripheral drive devices in which various processes for microbial analysis are integrated as an embodiment of the microbial analysis apparatus 200 of the present invention. .
디스크(100)는 상부 몸체(1)와 하부 몸체(2)로 구성되며, 박막 접착 테이프(2a)에 의해 서로 밀착 부착되어 하나의 디스크 (100)를 이룬다.The disk 100 is composed of the upper body 1 and the lower body 2, and is adhered to each other by a thin film adhesive tape (2a) to form a single disk (100).
도면 부호 120은 검체를 주입하기 위한 디스펜서(dispensor), 피펫, 주사위, 샘플러(sampler), 란셋(lancet) 중 선택된 검체 주입 수단을 나타내고, 도면 부호 121은 검체를 주입하기 위한 검체 주입구를 나타낸다. 도면 부호 130은 검체 주입 수단(120)에 의해 주입된 검체를 일시 저장하고 프렙 공정을 수행하기 위한 프렙 챔버이며, 도면 부호 132는 검체 내의 미생물에 대해 영양분을 제공하는 배양 공정을 위한 배지(culture medium)를 제공하는 배지 챔버이며, 도면 부호 133은 찌꺼기 및 과잉 검체를 모으는 세정공정을 위한 트레쉬 챔버이며, 그리고 도면 부호 70, 71은 상기 챔버들 사이에 유체 및 검체를 이동시키기 위한 밸브를 나타낸다. 또한 도면 부호 170은 디스크 공극을 나타낸다. 도면 부호 335는 몸체의 기준을 나타내는 기준구멍을 나타낸다. Reference numeral 120 denotes a sample injection means selected from a dispenser, pipette, dice, sampler, and lancet for injecting a sample, and reference numeral 121 denotes a sample injection port for injecting a sample. Reference numeral 130 is a preparation chamber for temporarily storing a sample injected by the sample injection means 120 and performing a preparation process, and reference numeral 132 denotes a culture medium for a culture process for providing nutrients to the microorganisms in the sample. ) Is a media chamber, a reference numeral 133 denotes a trash chamber for a cleaning process for collecting the residue and excess sample, and reference numerals 70 and 71 denote a valve for moving the fluid and the sample between the chambers. Also, reference numeral 170 denotes a disk gap. Reference numeral 335 denotes a reference hole indicating a reference of the body.
한편, 도면 부호 91은 상기 디스크의 제품 ID, 유효 기간, 분석 및 진단할 수 있는 미생물의 종류 등에 관한 정보가 포함될 수 있는 바코드이다. 이미지 센서(103)는 디스크 상의 바코드(91)를 판독할 뿐만 아니라, 상기 배지 챔버(132)에서의 미생물 배양 결과를 정량 분석 혹은 정성 분석하기 위해 배지 챔버(132)를 촬영하여 배지 챔버(132)의 칼라 이미지를 얻는다. On the other hand, reference numeral 91 is a bar code that may include information about the product ID, expiration date, the type of microorganism that can be analyzed and diagnosed, etc. of the disc. The image sensor 103 not only reads the barcode 91 on the disc, but also photographs the medium chamber 132 to quantitatively or qualitatively analyze the microorganism culture result in the medium chamber 132. Get a color image of
상기 프렙 챔버(130)에는, 검체 내에 존재하는 미생물을 증균하기 위한 액체 배양액(liquid culture medium)을 포함하거나 이를 저장하기 위한 증균 챔버를 더 포함할 수 있다.The preparation chamber 130 may further include an enrichment chamber for containing or storing a liquid culture medium for enriching the microorganisms present in the sample.
도면부호 211은 상기 레이저 빔 발생장치(5a)를 탑재한 스라이더(slider)로 스라이드(slide) 모터(109)와 연결되어 구동 제어되며 스라이더의 이동 제어에 의해 상기 버스트 밸브에 대한 레이저 빔 발생장치(5a)의 방사 방향의 공간 어드레싱(space addressing)이 이루어진다. Reference numeral 211 denotes a slider mounted on the laser beam generator 5a and connected to a slide motor 109 to be driven and controlled to generate a laser beam for the burst valve by movement of the slider. Radial space addressing of the device 5a is achieved.
상기 방사방향의 공간 어드레싱은 상기 스라이더를 방사방향으로 가역적 이동 시킬 수 있는 스라이드 모터(109)에 의한다. The radial space addressing is by means of a slide motor 109 capable of reversibly moving the slider in the radial direction.
상기 스라이더 모터의 회전에 의해 스라이더는 몸체의 중심으로부터 외곽방향으로 또는 몸체의 외곽으로부터 몸체의 중심으로 방사방향 이동할 수 있다.The rotation of the slider motor allows the slider to move radially outward from the center of the body or from the outside of the body to the center of the body.
상기 각각의 공정(프렙 공정, 증균 공정, 배양 공정, 세정 공정)의 시작시점과 종료 시점에서의 밸브의 개폐 제어는 버스트 밸브에 의해 이루어진다. 구체적으로는 상기 스라이더(slider)(211) 상에 설치된 레이저 빔 발생장치(5a)를 해당 밸브의 반경으로 공간 이동함과 동시에 레이저 빔을 온(On)한 상태에서 몸체(100)를 회전시켜 버스트 밸브를 가열함으로 회전 동안 원심력에 의해 밸브가 개방된다. 이때 상기 레이저 빔 발생장치(5a)는 상기의 "펄스 빔" 또는 "스캔닝 빔" 모드로 동작한다.The opening and closing control of the valve at the start point and the end point of each of the above processes (prep process, enrichment process, culture process, washing process) is performed by a burst valve. Specifically, the body 100 is rotated while the laser beam generator 5a installed on the slider 211 is spatially moved to the radius of the valve and the laser beam is turned on. Heating the burst valve opens the valve by centrifugal force during rotation. At this time, the laser beam generator 5a operates in the "pulse beam" or "scanning beam" mode.
도면부호 110b은 스라이더(211)상의 레이저 빔 발생장치(5a) 및 이미지 센서(103)에 필요한 각종 제어신호를 연결키 위한 프렉셔블(flexible) 케이블(cable)로 웨이퍼(wafer) 혹은 하네스(harness)(110a)를 통해 중앙제어장치(105)와 연결된다. Reference numeral 110b is a flexible cable for connecting various control signals required for the laser beam generator 5a and the image sensor 103 on the slider 211 to a wafer or a harness. 110a is connected to the central control unit 105.
도면 부호 181은 디스크(100)를 올려놓기 위한 턴 테이블(turn table)로, 상기 디스크는 중심 공극(170)을 통해 턴 테이블에 프런트(front) 혹은 탑로딩된다. Reference numeral 181 denotes a turn table for placing the disk 100, which is front or top loaded on the turn table through the central void 170.
도면 부호 188은 메모리 내장형 무선 RF IC 혹은 전자태그(tag)장치로 미생물 분석을 위한 프로토콜, 분석 알고리즘, 판독을 위한 표준 제어값을 포함한다. 또한 개인 암호화 정보 및 미생물 분석장치의 ID(identification)가 저장될 수 있어, 타인이 함부로 사용할 수 없도록 할 수 있다. Reference numeral 188 is a memory-embedded wireless RF IC or electronic tag device that includes protocols for analyzing microorganisms, analysis algorithms, and standard control values for reading. In addition, personal encryption information and identification (identification) of the microbial analysis device can be stored, so that others can not be used without permission.
상기 무선 RF IC(188)는 스마트 IC카드 형태가 선호된다. 상기 무선 RF IC(188) 정보는 무선 송수신을 통해 중앙제어장치(105)에 제공되며, 개인 암호화를 위해 활용된다. 도면부호 110은 상기 무선 RF IC(188)에 전원을 공급키 위한 무선전파 발생부이다. 상기 무선전파 발생부에 의한 전파는 플레밍의 법칙에 따라 무선RF IC(188)속에 내장된 유도　코일을 감응시켜 충분한 양의 전기를 생산해 무선 RF IC(188)에 전원을 공급한다. The wireless RF IC 188 is preferably in the form of a smart IC card. The wireless RF IC 188 information is provided to the central control unit 105 through wireless transmission and reception, and is utilized for personal encryption. Reference numeral 110 is a radio wave generation unit for supplying power to the wireless RF IC 188. The radio wave generated by the radio wave generator generates a sufficient amount of electricity by supplying a power to the radio RF IC 188 by sensitizing the induction coil coil embedded in the radio RF IC 188 according to Fleming's law.
본 발명의 미생물 분석장치에 있어서, 바람직하게는 상기 무선 RF IC(188)는 온도 측정기능이 있어 배지 챔버의 온도를 계측하여 중앙제어장치(105)에 무선송신하는 것을 특징으로 한다. 배지 챔버(132)의 온도가 높거나 낮으면 중앙제어장치(105)는 가열(heating) 수단(240) 혹은 냉각(cooling) 수단에 의해 일정한 온도를 유지토록 한다. 본 발명에 있어서 배지 챔버(132)의 온도는 미생물 증식에 적합한 섭씨 35도에서 40도 사이 중 선택된 온도를 유지하는 것이 선호된다. 상기 냉각 수단은 몸체(100)의 회전 혹은 회전 팬(fan)에 의한 것이 선호된다. In the microbial analysis apparatus of the present invention, preferably, the wireless RF IC 188 has a temperature measuring function, and measures the temperature of the medium chamber to wirelessly transmit to the central control unit 105. When the temperature of the medium chamber 132 is high or low, the central control unit 105 maintains a constant temperature by the heating means 240 or the cooling means. In the present invention, the temperature of the medium chamber 132 is preferably maintained at a temperature selected between 35 degrees Celsius and 40 degrees Celsius suitable for microbial growth. The cooling means is preferably by rotation or rotating fan of the body 100.
가열된 상기 배지 챔버(132)의 온도를 바람에 의해 냉각하기 위해 디스크를 회전시키거나 또는 회전팬을 돌리는 한편 상기 RF IC에 의해 측정된 온도를 기준으로 하여 원하는 온도까지 배지 챔버(132)를 냉각시키기 위해 상기 디스크(100)를 더 회전시키는 것이 선호된다. 이를 위해 상기 무선 RF IC(188)는 상기의 배지 챔버(132)의 온도를 판독하여 그 결과를 중앙제어장치(105)로 무선 송신하는 것을 특징으로 한다. The disk is rotated or the fan is rotated to cool the heated medium chamber 132 by wind while cooling the medium chamber 132 to a desired temperature based on the temperature measured by the RF IC. It is preferred to rotate the disk 100 further to make it work. To this end, the wireless RF IC 188 is characterized in that the temperature of the discharge chamber 132 is read and wirelessly transmits the result to the central control unit 105.
본 발명의 미생물 분석장치에 있어서, 바람직하게는 상기 가열수단은 RF IC의 출력단에 연결된 나노(nano) 패턴(240)이 선호되며 RF IC의 전력 공급의 온오프 간격과 전력량에 의해 가열 수단(240)의 온도가 제어되는 것이 선호된다. In the microbial analysis apparatus of the present invention, preferably, the heating means is preferably a nano pattern 240 connected to an output terminal of the RF IC, and the heating means 240 is controlled by on / off intervals and power amounts of power supply of the RF IC. It is preferred that the temperature of is controlled.
본 발명의 또 다른 측면은, 상기 가열수단은 상기 레이저 빔 발생장치(5a)의 스캔닝 빔 동작에 의해 이루어지는 것을 특징으로 한다.Another aspect of the invention is characterized in that the heating means is made by a scanning beam operation of the laser beam generator 5a.
본 발명의 미생물 분석장치에 있어서, 바람직하게는 상기 무선 RF IC(188)는 미생물 분석장치의 검사에 따른 검사일자 및 검사 결과, 검사의 유효기간, 미생물 분석 결과 등에 대한 정보가 포함되어 있는 것을 특징으로 한다. 미생물 분석 후, 미생물 분석장치를 RF IC 판독기에 갖다대거나 혹은 몸체(100)를 미생물 분석장치에 로딩함으로써 그에 대한 정보를 재확인할 수 있다. 상기 미생물 분석 결과는 상기 이미지 센서(103)에 의해 얻어진 배지 챔버(132)의 칼라 이미지가 선호된다. 상기 검사 결과는 상기 배지 챔버(132)의 칼라 이미지를 분석하여, 미생물 종류에 따른 집락 밀도 및 집락의 개체수(population)가 선호된다.In the microbial analysis device of the present invention, preferably, the wireless RF IC 188 includes information on an inspection date and a test result, an expiration date of the test, and a microbial analysis result according to the test of the microbial analysis device. It is done. After the microbial analysis, the information on the microbial analysis device may be brought to the RF IC reader or the body 100 may be loaded into the microbial analysis device. The microorganism analysis result is preferably a color image of the medium chamber 132 obtained by the image sensor 103. The test results analyze the color image of the medium chamber 132, the colony density and population of the colony (population) according to the type of microorganism is preferred.
본 발명의 미생물 분석장치는 미생물의 관찰하기 위한 현미경 센서(106)와 상기 현미경 센서로부터의 이미지를 처리하고 콜로니를 계수하거나 세포의 생사 여부를 확인하기 위한 중앙제어장치(105)를 내장하는 것이 선호된다.The microorganism analyzing apparatus of the present invention preferably includes a microscopic sensor 106 for observing microorganisms and a central controller 105 for processing an image from the microscopic sensor and counting colonies or checking whether cells are dead. do.
본 발명의 미생물 분석장치에 있어서, 바람직하게는 상기 중앙제어장치(105)는 디스크(100)의 검사결과, 검사일자 및 미생물 분석 결과를 무선 RF IC(188)에 내장된 메모리 또는 저장장치(113)에 저장하는 것을 특징으로 한다.In the microorganism analyzing apparatus of the present invention, preferably, the central control unit 105 stores the test result, the test date, and the microbial analysis result of the disk 100 in the memory or storage device 113 embedded in the wireless RF IC 188. It is characterized by storing in).
본 발명의 미생물 분석장치에 있어서, 바람직하게는 상기 입출력장치는 USB(Universal Serial Bus) 혹은 IEEE1394 혹은 ATAPI 혹은 SCSI 혹은 인터넷 통신망의 통신 규격을 갖는 것을 특징으로 한다. 또한 상기 입출력장치(111)를 통해, 검체의 품명, 검체의 수집 장소, 검체의 수집 일시등 검체 자체에 대한 정보들을 입력할 수 있다.In the microbial analysis device of the present invention, preferably, the input / output device has a communication standard of USB (Universal Serial Bus) or IEEE 1394 or ATAPI or SCSI or Internet communication network. In addition, through the input and output device 111, information about the sample itself, such as the name of the sample, the collection place of the sample, the collection date and time of the sample can be input.
도 9는 상기 레이저 빔 발생장치(5a)와 이미지 센서(103)가 설치 배치된 스라이더(slider)(211)의 윗 도면의 일실시예를 나타낸다. 상기 스라이더(slider)는 스라이드(slide) 모터(109) 축에 연결된 웜(worm) 기어 연결부(109a, 109b)에 의해 이동 제어된다.FIG. 9 shows an embodiment of the upper view of a slider 211 in which the laser beam generator 5a and the image sensor 103 are installed. The slider is movement controlled by worm gear connections 109a and 109b connected to the axis of the slide motor 109.
상기 스라이더(slider)는 스라이드 아암(108a, 108b)을 가이드(guide)로 사용하여 미끄러지듯 이동된다. 상기 스라이드 아암(108a, 108b)은 나사(110a 내지 110d)를 통해 미생물분석장치(200)의 몸체에 체결된다. 도면 부호 110b은 플렉셔블 케이블(flexible cable)이며 웨이퍼 혹은 하네스(110a)를 통해 연결된다. 도면 부호 181은 상기의 스핀들(spindle) 모터(102)에 의해 회전하는 턴 테이블이다.The slider is slidably moved using the slide arms 108a and 108b as guides. The slide arms 108a and 108b are fastened to the body of the microbial analysis apparatus 200 through screws 110a to 110d. Reference numeral 110b denotes a flexible cable and is connected through a wafer or harness 110a. Reference numeral 181 denotes a turn table that is rotated by the spindle motor 102 described above.
도 10은 본 발명의 일 실시예에 따른 미생물 분석장치(200)의 전체 단면도이다. 여기서, 도면 부호 200a는 미생물 분석장치(200)를 지지하고 있는 외곽몸체이다. 미생물 분석장치(200)의 밑면에는 회로기판(140)이 상기 외곽몸체(200a)에 이음 체결되어있고, 회로 기판 위에 미생물 분석장치(200)를 제어하기 위한 중앙제어장치(105), 저장장치(113) 및 입출력장치(111)가 상기 회로 기판(140) 위에 배치 설계되어 있다. 10 is a cross-sectional view of the microbial analysis apparatus 200 according to an embodiment of the present invention. Here, reference numeral 200a denotes an outer body supporting the microorganism analyzing apparatus 200. A circuit board 140 is jointly fastened to the outer body 200a at the bottom of the microorganism analyzing apparatus 200, and a central controller 105 and a storage device for controlling the microorganism analyzing apparatus 200 on the circuit board. 113 and an input / output device 111 are designed to be disposed on the circuit board 140.
상기 중앙제어장치(105)는 1) 디스크(100)의 회전 혹은 정지를 위해 스핀들 모터(102)를 제어할 뿐만 아니라, 2) 스라이드 모터(109) 제어에 의해 스라이더(211) 상에 설계 배치된 이미지 센서(103)의 이동을 제어뿐만 아니라, 3) 밸브 개폐 동작시 밸브에 열을 가하기 위해 레이저 빔 발생장치(5a)의 위치를 해당 밸브의 반경 위치로 이동시키는 역할을 수행한다. 이때, 이미지 센서(103)는 스라이더(211) 상에 탑재되거나 회로기판(140)상에 배치될 수 있다. 도면에는 스라이더(211) 상에 이미지 센서(103)가 탑재된 것만 표시하였음을 유의한다.The central controller 105 1) not only controls the spindle motor 102 for rotation or stop of the disk 100, but 2) design arrangement on the slider 211 by the control of the slide motor 109. In addition to controlling the movement of the image sensor 103, 3) serves to move the position of the laser beam generator 5a to the radial position of the valve in order to apply heat to the valve during the valve opening and closing operation. In this case, the image sensor 103 may be mounted on the slider 211 or disposed on the circuit board 140. Note that only the image sensor 103 is mounted on the slider 211.
또한 중앙제어장치(105)는 현재 미생물 분석 장치(200)에 로딩된 디스크가 미생물 분석용인지 여부를 판단한다.In addition, the central control unit 105 determines whether the disk currently loaded in the microbial analysis apparatus 200 is for microbial analysis.
본 발명에서, 바람직하게는 상기 디스크(100)가 미생물 분석 장치(200)에 로딩되는 시점에서 상기 무선 RF IC(188)를 통해, 상기 중앙 제어 장치(105)에 디스크(100)의 고유 ID를 무선 송신토록 함으로써, 현재 미생물 분석 장치(200)에 로딩된 디스크(100)가 미생물 분석용 디스크인지 여부를 중앙제어장치(105)가 인식하도록 하는 것을 특징으로 한다. In the present invention, preferably the unique ID of the disk 100 to the central control unit 105 via the wireless RF IC 188 at the time when the disk 100 is loaded into the microbial analysis apparatus 200. By wireless transmission, it is characterized in that the central control unit 105 recognizes whether the disk 100 currently loaded in the microbial analysis apparatus 200 is a disk for microbial analysis.
본 발명 또 다른 측면은, 상기 디스크(100)가 미생물 분석 장치에 로딩되는 시점에서 디스크 상의 바코드를 상기 이미지 센서(103)에 의해 센싱하고 이를 상기 중앙 제어 장치(105)에 의해 분석함으로써, 현재 미생물 분석 장치(200)에 로딩된 디스크가 미생물 분석용 디스크인 것을 중앙제어장치(105)가 인식하도록 하는 것을 특징으로 한다. Another aspect of the present invention, by sensing the barcode on the disk by the image sensor 103 at the time when the disk 100 is loaded into the microbial analysis device and by analyzing it by the central control device 105, The central controller 105 recognizes that the disk loaded in the analysis device 200 is a disk for analyzing microorganisms.
본 발명 또 다른 측면은, 상기 디스크(100)가 미생물 분석 장치에 로딩되는 시점에서 디스크 상의 특정 표시(mark) 내지 무늬(pattern)를 상기 이미지 센서(103)에 의해 센싱하고 이를 상기 중앙 제어 장치(105)에 의해 분석함으로써, 현재 미생물 분석 장치(200)에 로딩된 디스크가 진품의 미생물 분석용 디스크인지 여부를 중앙제어장치(105)가 인식하도록 하는 것을 특징으로 한다.According to another aspect of the present invention, a specific mark or pattern on the disk is sensed by the image sensor 103 at the time when the disk 100 is loaded into the microbial analysis apparatus and the central control device ( By analyzing by 105, it is characterized in that the central control unit 105 recognizes whether the disk currently loaded in the microbial analysis apparatus 200 is a genuine microbial analysis disk.
상기 이미지 센서(103)에 의해 얻어진 배지 챔버(132)에 대한 이미지 정보는 상기 스라이더(211)에 연결된 flexible cable(110b)를 통해 중앙 제어 장치(105), 저장장치(113) 혹은 입출력장치(111)로 보내질 수 있다.The image information about the discharge chamber 132 obtained by the image sensor 103 may be transferred to the central control unit 105, the storage unit 113, or the input / output unit through the flexible cable 110b connected to the slider 211. 111).
상기 스라이더(211)상의 이미지 센서(103) 또는 회로기판(140) 위에 배치설계된 이미지 센서에 의해 얻어진 배지 챔버(132)에 대한 이미지 정보는 중앙 제어 장치(105), 저장장치(113) 혹은 입출력장치(111)에 보내진다. The image information of the discharge chamber 132 obtained by the image sensor 103 on the slider 211 or the image sensor arranged and designed on the circuit board 140 may be transmitted to the central control unit 105, the storage unit 113, or the input / output unit. Is sent to the device 111.
도면 부호 104는 디스크 공극(170)에 로딩된 디스크(100)의 압착수단으로 강자성체(104a)와의 자력인력에 의해 압착되며 수직이동과 공회전이 가능하도록 설계되는 것이 선호된다. 또한 강자성체(181a)는 턴테이블(181)과의 자력인력에 의해 압착되는 것이 선호된다. 상기 턴테이블(181)과 압착수단(104)은 영구 자석이 선호된다. Reference numeral 104 is a crimping means of the disk 100 loaded in the disk cavity 170 is preferably pressed by the magnetic force with the ferromagnetic material (104a) is preferably designed to enable vertical movement and idle rotation. In addition, the ferromagnetic material (181a) is preferably compressed by the magnetic force with the turntable (181). The turntable 181 and the crimping means 104 are preferably permanent magnets.
상기 스라이더(211)는 이동 가능한 영구자석(5b)을 더 탑재할 수 있다.The slider 211 may further mount a movable permanent magnet 5b.
바람직하게는 상기 이미지 센서(103)가 배지 챔버(132)에 대한 이미지를 캡쳐하기 전에 "배지 챔버의 탐색 과정"을 진행하는 것을 특징으로 한다. Preferably, the image sensor 103 performs a "search process of the discharge chamber" before capturing an image for the discharge chamber 132.
본 발명의 미생물 분석 장치에 있어서, 바람직하게는 상기 이미지 센서 (103)와 배지 챔버(132)간의 광학적 정렬(optical alignment)을 용이케 하기 위해 몸체(100)상에 영구자석(5c)을 구비한 것을 특징으로 한다.In the microbial analysis apparatus of the present invention, the permanent magnet 5c is provided on the body 100 to facilitate optical alignment between the image sensor 103 and the medium chamber 132. It is characterized by.
상기 스핀들 모터(102)에 의한 몸체(100)의 짧은 회전 동안, 영구자석(5c)과 이동 가능한 자석(5b)이 서로 만나면 두 자석간의 인력에 의해 몸체(100)는 더 이상 회전되지 않고, 상기 이미지 센서(103)와 배지 챔버(132)간의 광학적 정렬(optical alignment)이 이루어 짐으로써 상기 "배지 챔버의 탐색 과정"이 완료된다. 상기 짧은 회전은 0.1초 내지 0.5초간의 회전이 선호된다.During the short rotation of the body 100 by the spindle motor 102, when the permanent magnet 5c and the movable magnet 5b meet each other, the body 100 is no longer rotated by the attraction force between the two magnets. The optical alignment between the image sensor 103 and the discharge chamber 132 is completed to complete the "search process of the medium chamber". The short rotation is preferably a rotation of 0.1 second to 0.5 seconds.
도 11은 상기 턴테이블(181), 강자성체(181a)와 압착수단(104)에 대한 또 다른 일 실시예이다. 상기 강자성체(181a)는 상기 턴테이블(181)의 노브(181c)(knob)와 기계적으로 맞물릴 수 있도록 홈(181b)을 갖는 경우를 보인다. 11 is yet another embodiment of the turntable 181, the ferromagnetic material (181a) and the pressing means 104. The ferromagnetic material 181a has a groove 181b to be mechanically engaged with knobs 181c and knobs of the turntable 181.
도면 부호 40는 상기 이미지 센서의 조명(illumination)을 위한 적어도 하나 이상의 LED(light Emitting Diode)이며, 상기 이미지 센서(103) 혹은 LED는 스라이더(211) 상에 탑재되거나 배지 챔버(132)의 상측 혹은 하측에 설치될 수 있다. Reference numeral 40 is at least one light emitting diode (LED) for illumination of the image sensor, the image sensor 103 or the LED is mounted on the slider 211 or the upper side of the discharge chamber 132 Or it can be installed at the bottom.
상기 이미지 센서(103)는 CCD 혹은 CMOS 혹은 픽셀(pixel) 단위로 광량을 센싱하는 선형 이미지 센서(line image sensor)가 선호된다. 본 발명에서, 상기 선형 이미지 센서는 리니어 센서 어레이(linear sensor array) 혹은 CIS(Contact Image Sensor)가 선호된다.The image sensor 103 is preferably a linear image sensor that senses the amount of light in a CCD or CMOS or pixel unit. In the present invention, the linear image sensor is preferably a linear sensor array or a contact image sensor (CIS).
본 발명에서, 상기 이미지 센서(103)는 배지 챔버(132)의 이미지 정보를 얻기 위해 상기 스라이더(211)를 배지 챔버(132)에 해당하는 반경으로 이동시키는 것을 특징으로 한다. In the present invention, the image sensor 103 is characterized in that to move the slider 211 to the radius corresponding to the discharge chamber 132 to obtain the image information of the discharge chamber 132.
도 12 내지 도 15는 미생물 분석을 위한 제반 공정이 집적화된 디스크(100) 실시예 및 미생물 분석 장치의 사용예를 포함한다. 이하 이들 도면을 참조하여 설명한다. 12 to 15 include the embodiment of the disk 100 integrated with the overall process for microbial analysis and the use of the microbial analysis apparatus. A description with reference to these drawings is as follows.
본 실시예에서는 디스크(100)가 상부 몸체(1)와 하부 몸체(2)가 접착테이프(2a)에 의해 적층 결합되고 밸브로서 상기 수압 버스트 밸브가 채용된 실시예를 보인다.In this embodiment, the disk 100 is an embodiment in which the upper body 1 and the lower body 2 are laminated and bonded by an adhesive tape 2a, and the hydraulic burst valve is employed as a valve.
도면부호 121a, 121b, 121c 및 121d은 검체를 주입하기 위한 검체 주입구를 나타내고, 또한 상기 디스크(100)는 상기 검체 주입구를 통해 주입된 검체를 일시 저장하기 위한 적어도 하나 이상의 프렙 챔버(130a 내지 130d); 상기 검체 내의 미생물에 대해 영양분을 제공하기 위한 배지(80)를 제공하는 적어도 하나 이상의 배지 챔버(132a, 132b, 132c, 132d); 찌꺼기 및 과잉 검체를 모으기 위한 트레쉬 챔버(133a, 133b, 133c, 133d); 및 상기 챔버들 사이에 유체 및 검체를 이동시키기 위한 밸브(70a, 70b, 70c, 70d, 71a, 71b, 71c 및 71d)를 포함한다. 도면 부호 170은 디스크 공극을 나타낸다. 도면 부호 335는 몸체의 기준을 나타내는 기준구멍이다. Reference numerals 121a, 121b, 121c, and 121d indicate sample inlets for injecting a sample, and the disk 100 includes at least one prep chamber 130a to 130d for temporarily storing a sample injected through the sample inlet. ; At least one medium chamber (132a, 132b, 132c, 132d) providing a medium (80) for providing nutrients to the microorganisms in the sample; Trash chambers 133a, 133b, 133c, and 133d for collecting debris and excess samples; And valves 70a, 70b, 70c, 70d, 71a, 71b, 71c and 71d for moving the fluid and the sample between the chambers. Reference numeral 170 denotes a disk gap. Reference numeral 335 denotes a reference hole indicating a reference of the body.
각각의 프렙 챔버(130a, 130b, 130c, 130d)에는 다른 종류 또는 같은 종류의 검체를 주입할 수 있다. 또한 상기 배지 챔버(132a, 132b, 132c, 132d)에는 같은 배지 또는 다른 종류의 배지를 내장할 수 있다. 따라서 상기 디스크(100)를 가지고 상기 단일검체에 대한 다종 미생물 검사 및 다종검체에 대한 단일 미생물 검사가 가능하다. Each prep chamber 130a, 130b, 130c, 130d may be injected with different or the same kind of sample. In addition, the medium chambers 132a, 132b, 132c, and 132d may contain the same medium or different types of medium. Therefore, with the disk 100, it is possible to test for multiple microorganisms on the single specimen and to test a single microorganism on the multiple specimens.
프렙 챔버(130a 내지 130d)내의 검체를 배지 챔버(132a 내지 132d)로 이송하기 위해서는 내측에 설치된 수압 버스트 밸브(70a 내지 70d)를 각각 개방하여야 한다.In order to transfer the specimens in the preparation chambers 130a to 130d to the medium chambers 132a to 132d, the hydraulic burst valves 70a to 70d provided therein must be opened, respectively.
상기 외측에 설치된 수압버스트 밸브(71a 내지 71d)는 내측에 설치된 수압버스트 밸브(70a 내지 70d)보다 원심력이 다 강하게 작용하므로 내측에 설치된 수압버스트 밸브(70a 내지 70d)가 먼저 개방되기 위해서는 내측 설치된 수압버스트 밸브(70a 내지 70d)의 박막 접착테이프의 접착 면적을 외측에 설치된 수압 버스트 밸브(71a 내지 71d)보다 더 크게 하여 폐쇄 강도(closing strength)를 강하게 해주든지 내측에 설치된 수압 버스트 밸브(70a 내지 70d) 개방시 상기 레이저 빔 발생장치(5a)에 의해 내측에 설치된 수압 버스트 밸브(70a 내지 70d)를 "펄스 빔" 또는 "스캔닝 빔" 동작에 의해 가열해주어야 한다. Since the centrifugal force acts more strongly than the hydraulic pressure valves 71a to 71d installed on the outside, the internal pressures to open the hydraulic pressure valves 70a to 70d installed on the inside first. The adhesion area of the thin-film adhesive tape of the burst valves 70a to 70d is larger than the hydraulic burst valves 71a to 71d provided on the outside to increase the closing strength, or the hydraulic burst valves 70a to 70d provided on the inside. When opening, the hydraulic burst valves 70a to 70d installed inside by the laser beam generator 5a should be heated by a "pulse beam" or "scanning beam" operation.
상기 수압 버스트 밸브(70a 내지 70d)의 개방에 의해 배지 챔버(132)내로 이동한 검체는 배지(80) 표면을 골고루 도포하여 접종원이 배지에 접종이 되도록해야 한다. Samples moved into the medium chamber 132 by opening the hydraulic burst valves 70a to 70d should be evenly applied on the surface of the medium 80 so that the inoculum is inoculated onto the medium.
배지 챔버(132a 내지 132d)의 내측 높이(131a)는 외측 높이 (131b) 보다 챔버의 높이가 더 작다. 따라서 상기 배지 챔버 내에 유입된 검체는 모세관 현상에 의해 배지 챔버의 원중심 방향으로 모이게 된다. 이후, 디스크를 회전시키면 원심력에 의해 검체는 배지 챔버의 방사방향 의 외측으로 이동하게 되고 이후 디스크(100)의 회전을 멈추면 모세관 현상에 의해 배지 챔버의 내측으로 검체가 다시 이동하게 된다. 이것을 수차례 반복하면, 배지(80)의 표면에 검체가 골고루 도포되게 된다. 이후, 버스트 밸브(71a 내지 71d)을 개방하여 과잉 검체를 트레쉬 챔버(133a 내지 133d)로 이동시킨다. The inner height 131a of the discharge chambers 132a to 132d is smaller in height than the outer height 131b. Therefore, the sample introduced into the medium chamber is collected in the direction of the center of the medium chamber by the capillary phenomenon. Subsequently, when the disk is rotated, the sample is moved outward in the radial direction of the medium chamber by centrifugal force. Then, when the disk 100 is stopped rotating, the sample is moved back inside the medium chamber by capillary action. If this is repeated several times, the sample is evenly applied to the surface of the medium (80). Thereafter, the burst valves 71a to 71d are opened to move the excess sample to the trech chambers 133a to 133d.
본 발명에서, 프렙 챔버(130a 내지 130d)는 친수성 코팅되는 것이 선호되며, 상기 내측에 설치된 수압 버스트 밸브(70a 내지 70d)는 상술된 "자석 버스트 밸브", "소수성 버스트 밸브" 및 "레이저 버스트 밸브" 중 어느 하나 이상으로 대체될 수 있음을 유의한다. 또한 상기 프렙 챔버(130a 내지 130d)의 챔버 높이를 배지 챔버(132a 내지 132d)의 챔버 높이 보다 작게 설계하는 것이 선호된다. 이러한 구성으로 인해, 검체주입동안 프렙 챔버 자체내의 모세관 현상에 의해 상기 배지 챔버로 검체가 유입되는 것을 방지할 수 있다.In the present invention, the prep chambers 130a to 130d are preferably hydrophilic coated, and the hydraulic burst valves 70a to 70d installed therein are the "magnetic burst valves", "hydrophobic burst valves" and "laser burst valves" described above. It may be replaced by any one or more of ". In addition, it is preferable to design the chamber height of the preparation chambers 130a to 130d to be smaller than the chamber height of the medium chambers 132a to 132d. Due to this configuration, it is possible to prevent the sample from flowing into the medium chamber by capillary phenomenon in the preparation chamber itself during sample injection.
본 발명에서, 트레쉬 챔버(133a 내지 133d)는 친수성 코팅되는 것이 선호되며, 상기 외측에 설치된 수압 버스트 밸브(71a 내지 71d)는 상술된 "자석 버스트 밸브", "소수성 버스트 밸브" 및 "레이저 버스트 밸브" 중 어느 하나 이상으로 대체될 수 있음을 유의한다. 또한 상기 트레쉬 챔버(133a 내지 133d)의 챔버 높이를 배지 챔버(132a 내지 132d)의 챔버 높이 보다 작게 설계하는 것이 선호된다. 이러한 구성으로 인해, 트레쉬 챔버로 일단 이동한 찌꺼기 및 과잉 검체는 트레쉬 챔버 자체 내의 모세관 현상에 의해 상기 배지 챔버로 역류되는 것을 방지될 수 있다. 도 13은 수압 버스트 밸브(71b)의 상세도이다.In the present invention, the trash chambers 133a to 133d are preferably hydrophilic coated, and the hydraulic burst valves 71a to 71d installed on the outside are the "magnetic burst valve", "hydrophobic burst valve" and "laser burst" described above. It can be noted that any one or more of "valve" may be replaced. In addition, it is preferable to design the chamber height of the thresh chambers 133a to 133d to be smaller than the chamber height of the discharge chambers 132a to 132d. Due to this configuration, the debris and excess sample once moved to the tresh chamber can be prevented from flowing back to the medium chamber by capillary action in the trech chamber itself. 13 is a detailed view of the hydraulic burst valve 71b.
이하, 상기 배지(80)의 표면을 검체로 도포하고 과잉 검체를 트레쉬 챔버로 이동 시킴으로써 배지(80)에 접종이 이루어 지는 것을 "도포 접종"이라 칭한다. 상기 도포 접종에 의해, 기존 수작업에 의해 이루어 졌던 스트리킹(streaking) 작업을 자동화 할 수 있다. Hereinafter, the inoculation of the medium 80 by applying the surface of the medium 80 with a sample and moving the excess sample to the tresh chamber is referred to as "coating inoculation". By the application inoculation, it is possible to automate the streaking operation that has been done by conventional manual work.
이하, 도 12 내지 도 14를 참조하여 본 발명의 미생물 분석 장치의 사용 실시예를 설명한다.Hereinafter, an embodiment of using the microbial analysis apparatus of the present invention will be described with reference to FIGS.
우선, 채취봉(300a)을 샘플러(300)로부터 분리한다(단계 1). First, the sampling rod 300a is separated from the sampler 300 (step 1).
도면 부호 63과 63a는 채취봉(300a)과 배양튜브(300b) 간에 착탈이 가능토록 나사(screw) 체결부를 제공한다. 상기 채취봉(300a)의 말단에는 검사부위로부터 검체를 채취하기 위한 면봉(60)이 있다. 사용자는 의심되는 장소 내지 검사 부위(55)로부터 면봉(60)을 사용하여 검체를 채취한다. Reference numerals 63 and 63a provide a screw fastening portion to be detachable between the sampling rod 300a and the culture tube 300b. At the end of the sampling rod (300a) there is a cotton swab (60) for collecting the specimen from the inspection site. The user collects a sample using a swab 60 from a suspected place or inspection site 55.
그 후, 채취봉(300a)과 배양튜브(300b)를 나사(screw) 체결부(63,63a)를 통해 결합하고 24시간 증균 배양한다(단계 2). 상기 배양튜브(300b)에는 증균 배양액(69)이 보관되어 있어 미생물의 번식을 돕는다. 이때 증균 배양액은 펩톤수(peptone water)가 선호된다. 2단계 완료후, 배양 캡(67)을 샘플러(300)로부터 분리한다(단계 3). 도면 부호 67과 67a는 배양캡(67)와 배양튜브(300b)간에 착탈이 가능토록 나사(screw) 체결부를 제공한다. Then, the sampling rod (300a) and the culture tube (300b) is coupled through a screw fastening portion (63, 63a) and incubated for 24 hours enrichment (step 2). The culture tube (300b) is enriched culture medium 69 is stored to help the growth of microorganisms. At this time, the enrichment broth is preferably peptone water. After completion of step 2, the culture cap 67 is separated from the sampler 300 (step 3). Reference numerals 67 and 67a provide a screw fastening portion to be detachable between the culture cap 67 and the culture tube 300b.
그 후, 디스크(100)의 검체주입구(121)를 통해, 배양튜브(300b) 내에 있는 증균된 검체를 이송시킨다(단계 4). 이때 핸들(62)에 설치된 공기주머니(62a)를 손가락으로 눌러 배양튜브(300b) 내에 있는 증균된 검체를 상기 프렙 챔버(130a 내지 130d)로 이송시킨다. 도면 부호 69는 필터(filter)로 증균배양액(69)속에 있는 이물질이 디스크(100)로 이송되지 않도록 걸러주는 역할을 한다.Thereafter, the enriched sample in the culture tube 300b is transferred through the sample inlet 121 of the disc 100 (step 4). At this time, by pressing the air bag 62a installed on the handle 62 with a finger, the enriched sample in the culture tube 300b is transferred to the preparation chambers 130a to 130d. Reference numeral 69 serves to filter the foreign matter in the enrichment culture medium 69 to prevent the transfer to the disk 100 by a filter.
이 후, 프렙 챔버(130a 내지 130d) 내에 저장된 검체는 버스트 밸브(70a 내지 70d)의 개방에 의해, 배지 챔버(132a 내지 132d) 내로 이동하게 된다.Thereafter, the specimen stored in the preparation chambers 130a to 130d is moved into the medium chambers 132a to 132d by opening the burst valves 70a to 70d.
이 후, 상기 도포 접종 동작에 의해 상기 배지 챔버 내에 있는 배지의 표면을 검체에 의해 도포한다.Thereafter, the surface of the medium in the medium chamber is coated with a sample by the coating inoculation operation.
이 후, 버스트 밸브(71a 내지 71d)를 개방하여, 상기 배지 챔버 내의 잉여검체를 트레쉬 챔버(133a 내지 133d)로 이송한다.Thereafter, the burst valves 71a to 71d are opened to transfer the surplus specimens in the medium chamber to the trech chambers 133a to 133d.
이 후, 24시간 배지 챔버 내에서 미생물을 배양한다.Thereafter, the microorganisms are cultured in a media chamber for 24 hours.
이 후, 이미지 센서에 의해 배지 챔버을 판독하고, 상기 중앙제어장치에 의해 집락을 계수하여 미생물에 대한 정성 및 정량분석 한다.Thereafter, the medium chamber is read by the image sensor, and the colony is counted by the central controller to qualitatively and quantitatively analyze the microorganism.
이 후, 선택사항으로 상기 판독 결과에 따른 검사 결과를 컴퓨터 모니터 상에 표시되고, 자동 혹은 수동으로 인터넷 망을 통해 원격 접속되어 원격 전송된다.Thereafter, the test result according to the reading result is optionally displayed on the computer monitor, and remotely connected and remotely transmitted via the internet network automatically or manually.
원격지에서는 상기 검사결과를 바탕으로 사용자의 위생점수를 계산하여 상기 입출력 장치에 재전송하고 해당 위생점수가 사용자의 표시장치 내지 상점간판(signboard)에 표시된다. 이때, 위생점수는 검사 회수와 미생물의 집락 밀도를 기준으로 산정하는 것이 바람직하다. The remote site calculates the user's hygiene score based on the inspection result and retransmits the user's hygiene score to the input / output device. In this case, the hygiene score is preferably calculated based on the number of tests and the colony density of microorganisms.
도 15는 상기 배지 챔버 내에서 미생물을 배양된 결과를 보인 일례이다.15 is an example showing the result of culturing microorganisms in the medium chamber.
각 점(spot)들은 미생물의 집락을 나타내며, 크로모제닉 배지를 사용한 경우 미생물마다 각기 다른 색을 나타낸다.Each spot represents a colony of microorganisms and different colors for each microorganism when chromogenic media is used.
만약 미생물 분석 장치(100)에 의한 미생물 분석 중, 사용자에 의한 디스크의 추출(eject) 혹은 멈춤(stop) 요구시, 미생물 분석 장치는 이를 무시한 채 분석을 계속 진행한다. 이때 경고 메시지(warning message)를 사용자에게 알려주든가 패스워드를 요구한다. 이때 패스워드가 맞는 경우 사용자에 의한 디스크의 추출 혹은 멈춤 요구를 받아들인다.If during the microbial analysis by the microbial analysis apparatus 100, when the user ejects or stops the disk, the microbial analysis apparatus continues the analysis while ignoring it. At this time, a warning message is notified to the user or a password is required. If the password is correct, the user will be asked to eject or stop the disk.
또한 상기 무선 RF IC(188)의 메모리에는 디스크의 사용 이력, 유효기간 정보 혹은 분석할 수 있는 미생물의 종류에 관한 정보가 저장되어 있다. In addition, the memory of the wireless RF IC 188 stores a disk usage history, expiration date information or information on the types of microorganisms that can be analyzed.
즉, 미생물 분석 장치를 사용 중, 혹은 완료시 디스크를 미생물 분석 장치로부터 추출했을 때, 상기 무선 RF IC(188)의 메모리에 그것에 대한 이력(history)을 기록하여, 추후 미생물 분석 장치에 재 로딩하였을 때 사용 불가 디스크임을 사용자에게 알려준다.That is, when the disk is extracted from the microbial analysis device during use or completion of the microbial analysis device, a history of it is recorded in the memory of the wireless RF IC 188 and reloaded into the microorganism analysis device later. Tells the user that the disk is unavailable.
또한 상기 바코드 패턴은 디스크의 고유ID(identification) 정보 혹은 유효기간 정보 혹은 분석하고자 하는 미생물 종류가 저장되어 있다. 유효기간 정보에 의해 유효기간이 지난 디스크에 대해서도 사용 불가용임을 사용자에게 알려 준다. In addition, the barcode pattern stores the identification information or the expiration date information of the disk or the type of microorganism to be analyzed. The expiration date information informs the user that the expiration date is also unavailable for the disc.
도 16은 상기 도 14의 2단계에서 샘플러(300) 내의 미생물을 증균 배양하는 동안 최적의 항온(constant temperature)상태를 유지하기 위한 항온기 또는 온도제어장치(730)를 내장한 탑로딩 방식의 미생물 분석 장치(200)의 일 실시예를 도시한다.FIG. 16 is a toploading microorganism analysis incorporating a thermostat or a temperature control device 730 for maintaining an optimal constant temperature during enrichment of microorganisms in the sampler 300 in step 2 of FIG. One embodiment of the apparatus 200 is shown.
상기 증균 배양은 항온기 또는 온도제어장치 (730)에 샘플러(300)의 배양튜브(300b)를 삽입함으로써 이루어지는 것이 선호된다. The enrichment culture is preferably made by inserting the culture tube 300b of the sampler 300 into a thermostat or a temperature control device 730.
상기 디스크의 미생물 분석 장치(200)에 로딩은 탑로딩하기 위한 커버(751)를 열고 턴테이블(181)에 디스크(100)를 맞추어 끼워 넣으면 된다. The microorganism analysis apparatus 200 of the disk may be loaded by opening the cover 751 for top loading and fitting the disk 100 to the turntable 181.
도 17은 항온기 또는 온도제어장치를 내장한 프런트 로딩 방식의 미생물 분석 장치의 일실시예를 도시하는 도면으로써, 미생물을 증균 배양하는 동안 최적의 항온(constant temperature)상태를 유지하기 위해 온도센서(731), 항온기 또는 온도제어장치(730)를 트레이(tray)(761) 상에 매설한 탑로딩 방식의 미생물 분석 장치(200)의 일 실시예를 도시한다. FIG. 17 is a view showing an embodiment of a front loading type microorganism analyzing apparatus incorporating a thermostat or a temperature control device, and the temperature sensor 731 to maintain an optimal constant temperature during enrichment of microorganisms. ), An embodiment of a toploading microorganism analysis apparatus 200 in which a thermostat or a temperature controller 730 is embedded on a tray 761 is illustrated.
상기 증균 배양은 항온기 또는 온도제어장치(730)에 상기 몸체(100)의 공극(171)을 턴테이블(181)에 삽입하여 프런트 로딩함으로써 이루어지는 것이 선호된다. 도면 부호 733은 투명창으로, 배양기간 동안 외부에서 배양 상태를 관찰할 수 있도록 해준다.The enrichment culture is preferably made by front loading by inserting the void 171 of the body 100 to the turntable 181 in a thermostat or temperature control device 730. Reference numeral 733 is a transparent window, which allows to observe the culture state from the outside during the culture period.
도 16 및 도 17에 있어서, 미생물 분석 장치(200)는 분석 시작 버튼(745) 및 정지버튼(746)을 포함할 수 있다. 이때 도면 부호 742는 미생물 분석 장치의 전원 온오프 버튼이고, 도면 부호 741은 전원 상태를 표시하기 위한 LED이다. 도면 부호 760은 미생물 분석 장치(200)의 진행 상태를 표시하는 표시 장치이며, 이때 표시 장치로서 액정 표시장치(LCD)가 선호된다. In FIGS. 16 and 17, the microbial analysis apparatus 200 may include an analysis start button 745 and a stop button 746. In this case, reference numeral 742 denotes a power on / off button of the microbial analysis apparatus, and reference numeral 741 denotes an LED for indicating a power state. Reference numeral 760 denotes a display device for displaying a progress state of the microorganism analyzing apparatus 200, and a liquid crystal display (LCD) is preferred as the display device.
상기 표시장치(760)는 분석 결과를 표시해주거나 미생물 분석 장치의 주요공정에 따른 진행 상태를 표시하는 역할을 수행한다. 상기 표시장치(760)에 주요공정(프렙 공정, 증균 공정, 배양공정) 및 단계에 따른 진행률을 퍼센트(%) 혹은 막대 그래프의 형식으로 표시해 줄 수 있다. 또한 상기 표시장치(760)는 그래픽 사용자 인터페이스(Graphic User Interface) 제공할 수도 있다. 상기 그래픽 사용자 인터페이스는 사용자가 항온기에 대한 온도 설정, 배양 시간 설정을 제공하는 것이 선호된다. 또한 배양시간 경과시 이를 알람(alarm)을 통해 사용자에게 통지하는 것이 선호된다. The display device 760 displays an analysis result or displays a progress state according to a main process of the microbial analysis device. The display device 760 may display the progress according to the main process (prep process, enrichment process, culture process) and steps in the form of a percentage (%) or a bar graph. In addition, the display device 760 may provide a graphic user interface. The graphical user interface preferably allows the user to provide temperature settings, incubation time settings for the thermostat. It is also preferred to notify the user via an alarm when the incubation time has elapsed.
상기 온도제어장치는 히터 내지 PET(Polyethylene Terephthalate) 필름 위에 고저항체인 카본(carbon) 혹은 카본나노튜브(Carbon Nano tube)를 도포하여 카본 발열체에 의해 열을 발산하는 발열 필름을 트레이(761) 내부에 포함하는 것이 선호된다. The temperature control device applies a carbon or carbon nanotube, which is a high resistance material, onto a heater 761 in the tray 761 by applying a carbon or carbon nanotube, which is a high resistance material, on a heater or a PET (Polyethylene Terephthalate) film. It is preferred to include.
또한 본 발명에서는, 바람직하게는 상기 미생물 분석 장치는 배지 챔버의 이미지를 판독 분석하여 특정 미생물에 대한 음성, 양성 혹은 미생물 수치를 정량화하기 위한 계산 소프트웨어를 더 구비한 것을 특징으로 한다.In the present invention, preferably, the microbial analysis device is further provided with calculation software for quantifying negative, positive or microbial levels for a specific microorganism by reading and analyzing an image of the medium chamber.
도면 18은 상기 커버 수단이 몸체(100)의 최외주와 최내주에 설치된 일실시예를 표시한다. 상기 커버 수단은 상기 하부 몸체(2) 내에는 영구 자석(820b)이 설치되고 상부 몸체(1) 내에 강자성체(820a)가 설치되어 상부 몸체와 하부 몸체 간에 인력에 의해 닫히는 것이 선호된다. 배지 챔버(132a 내지 132d)내의 고체 배지에는 다량의 수분이 포함되어 있다. 만약 도면 16과는 다르게, 하부 몸체(2)에 배지를 설치한 채로 똑바로 배양하면 증발현상이 일어나 상부 몸체(1)의 천정에 습기가 차고 물방울이 맺히고, 급기야 물방울이 배지 위에 떨어져 배양 상태를 망가뜨릴 가능성이 있을 뿐만 아니라, 배양하는 동안 수분증발로 배지가 금방 말라 버릴 수 있다. 18 shows an embodiment in which the cover means is installed at the outermost and innermost circumferences of the body 100. It is preferred that the cover means is provided with a permanent magnet 820b in the lower body 2 and a ferromagnetic material 820a in the upper body 1 to be closed by the force between the upper body and the lower body. The solid medium in the medium chambers 132a to 132d contains a large amount of water. Unlike in FIG. 16, if the culture is carried out straight with the medium installed in the lower body 2, evaporation occurs, resulting in moisture and water droplets on the ceiling of the upper body 1, and the drop of water falls on the medium, thereby destroying the culture state. Not only is this possible, the medium can quickly dry out by evaporation during cultivation.
따라서 본 발명에서는 이러한 문제를 해결하기 위해, 도면 16에 도시된 바와 같이, 상부 몸체(1) 쪽에 배지(80)가 설치되는 것이 선호된다.Therefore, in the present invention, in order to solve this problem, it is preferable that the medium 80 is installed on the upper body 1 side, as shown in FIG.
또한 상부 몸체(1)쪽에 검체주입구(121a 내지 121d)가 설치되는 것이 선호된다. 상기 배지 챔버 내의 배지는 고체 배지 내지 아가 배지가 선호된다. 도면 부호 335는 몸체의 기준을 나타내는 기준구멍이다.In addition, it is preferred that the sample inlet (121a to 121d) is installed on the upper body (1) side. The medium in the medium chamber is preferably a solid medium to an agar medium. Reference numeral 335 denotes a reference hole indicating a reference of the body.
본 발명의 또 다른 측면은 상기 박막 접착 테이프(2a)와 강자성체(820a)가 자성체 시트(magnetic sheet)에 의해 대체되어 상기 커버 수단을 구성하는 것을 특징으로 한다. 이 경우 상기 자성체 시트의 한쪽면은 점착제가 코팅되어 있어 상부 몸체(1)에 부착되는 것이 선호된다.Another aspect of the invention is characterized in that the thin film adhesive tape 2a and the ferromagnetic material 820a are replaced by a magnetic sheet to constitute the cover means. In this case, one side of the magnetic sheet is coated with a pressure-sensitive adhesive is preferably attached to the upper body (1).
이 경우 상기 하부 몸체(2) 내의 영구 자석(820b)이 상부 몸체(1) 상의 자성체 시트에 대해 인력을 작용하여 상기 상부 몸체(1)와 하부 몸체(2)가 자력에 의해 결합되어 하나의 몸체(100)를 이룬다.In this case, the permanent magnet 820b in the lower body 2 acts on the magnetic sheet on the upper body 1 so that the upper body 1 and the lower body 2 are coupled by a magnetic force to form a single body. (100).
따라서 상기 자성체 시트는 박막 접착 테이프와 강자성체 역할을 동시에 수행한다. 즉, 상기 자성체 시트는 상기 상부 몸체(1)와 하부 몸체(2)의 조립 수단과 상기 커버 수단을 동시에 제공하는 장점이 있다. Therefore, the magnetic sheet serves as a thin film adhesive tape and a ferromagnetic material at the same time. That is, the magnetic sheet has an advantage of providing the assembly means and the cover means of the upper body 1 and the lower body 2 at the same time.
상기 자성체 시트는 쿠션 고무 재료와 자성체 분말이 박막 필름형태로 결합된 것이 선호된다. 상기 쿠션 고무 재료는 상기 상부 몸체(1)와 하부 몸체(2)가 자력에 의해 결합시 밀착력을 증대시킨다.The magnetic sheet is preferably a combination of a cushion rubber material and magnetic powder in the form of a thin film. The cushion rubber material increases the adhesion when the upper body 1 and the lower body 2 are coupled by magnetic force.
또한 자성체 시트는 검정색이기 때문에 레이저 빛에 대한 흡광률이 높아 상기의 레이저 버스트 밸브 구성에 매우 용이하다.In addition, since the magnetic sheet is black, the absorbance of the laser light is high, which is very easy to construct the laser burst valve.
상기 자성체 시트는 0.05mm 내지 0.1mm의 두께의 박막 형태가 선호된다.The magnetic sheet is preferably in the form of a thin film having a thickness of 0.05 mm to 0.1 mm.
도면 19는 전자석 보드를 사용한 개방수단의 일실시예를 보인다. 19 shows one embodiment of the opening means using an electromagnet board.
상기 하부 몸체 내의 영구 자석(820b)에 대해 척력을 발생시키는 전자석이 내장된 전자석 보드(330)에 의해 전자석 보드의 파워버튼(331) 온(ON)시 상기 하부 몸체(2) 내의 영구 자석(820b)에 대해 척력을 발생시켜, 하부 몸체(2)의 손쉬운 분리가 가능하다. 이때 몸체(100)를 전자석 보드(330)의 안착부위(332b)에 착탈이 시킨다. 도면 부호 332a 안착 테두리로 상기 몸체(100)의 모양을 따르는 것이 선호된다. The permanent magnet 820b in the lower body 2 when the power button 331 of the electromagnet board is turned on by the electromagnet board 330 having an electromagnet embedded therein that generates a repulsive force on the permanent magnet 820b in the lower body. By generating a repulsive force for), it is possible to easily remove the lower body (2). At this time, the body 100 is attached to and detached from the seating portion 332b of the electromagnet board 330. It is preferred to follow the shape of the body 100 with a seating edge 332a.
하부 몸체(2)를 몸체(100)로부터 분리 전에 몸체(100)를 전자석 보드(330)의 안착부위(332b)에 안착시킨다. 여기서 상기 몸체(100)는 도면 17에 도시된 바와 같이 배양때의 배치(도면16 참조)와는 달리, 하부 몸체와 상부 몸체의 상하배치를 바꾸어 안착하는 것이 선호된다.Before the lower body 2 is separated from the body 100, the body 100 is seated on the seating portion 332b of the electromagnet board 330. Here, the body 100, unlike the arrangement at the time of cultivation (see Fig. 16) as shown in Figure 17, it is preferred to seat by changing the top and bottom arrangement of the lower body and the upper body.
안착 후, 파워버튼(331) 온(ON)시 상기 전자석 보드 내에 내장된 전자석에 의해, 상기 하부 몸체(2) 내의 영구 자석(820b)에 대해 척력을 발생시켜, 몸체(100)로부터 하부 몸체(2)의 손쉬운 분리가 가능하다. 도면 부호 182는 몸체(100)를 올려놓기 위한 고정 테이블로, 중심 공극(170)을 통해 고정 테이블에 탑(top) 로딩된다. 상기 몸체가 상기 고정 테이블(182)의 기계적으로 맞물릴 수 있도록 홈(182b)을 갖는 경우를 보인다. After seating, the repulsive force is generated on the permanent magnet 820b in the lower body 2 by the electromagnet embedded in the electromagnet board when the power button 331 is ON, and the lower body ( 2) Easy separation is possible. Reference numeral 182 denotes a fixed table for placing the body 100, and is top loaded onto the fixed table through the center void 170. It is shown that the body has a groove 182b to be mechanically engaged with the fixed table 182.
동정(identification) 실험을 위한 군락 채집과정은 다음과 같이 진행된다. 우선 몸체(100)를 홈(182b)에 맞추어 끼워 넣은 후 파워버튼(331)을 켠 후, 몸체(100)로부터 하부 몸체(2)로 분리시켜 배지(80)로부터 의심되는 군락의 샘플을 채집한다. 이 채집된 군락은 동정(identification)실험에 사용될 것이다. 이후 파워버튼(331)을 오프(off)시킨후 하부 몸체(2)를 기준 기둥(334)에 맞추어 상부 몸체와 결합시킨다. 이후, 노브(182b)(knob)를 누르면 홈(182b)에 체결된 중심공극(170)이 풀려 몸체(100)를 전자석보드(330)로부터 빼낼 수 있다. 또한 안착 테두리(332a)상의 테두리 홈(333)은 손쉽게 몸체(100)를 전자석보드(330)로부터 끄집어낼 수 있도록 해준다. 상기 기준 기둥(334)은 전자보드상에서 이루어지는 몸체의 안착과 상부 몸체와 하부 몸체와의 재결합시 기준을 잡기 위한 것으로 상기의 기준 구멍(335)에 맞추어 끼워진다. The community gathering process for identification experiments proceeds as follows. First, the body 100 is fitted into the groove 182b, and then the power button 331 is turned on, and then separated from the body 100 into the lower body 2 to collect a sample of the suspected colony from the medium 80. . This collected community will be used for identification experiments. After the power button 331 is off (off), the lower body 2 is coupled to the upper body in accordance with the reference pillar 334. Thereafter, when the knobs 182b and the knobs are pressed, the central void 170 fastened to the groove 182b is released to remove the body 100 from the electromagnet board 330. In addition, the rim groove 333 on the seating edge 332a allows the body 100 to be easily pulled out of the electromagnet board 330. The reference column 334 is fitted to fit the reference hole 335 to set the standard when the body is mounted on the electronic board and recombination of the upper body and the lower body.
도면 20은 배지 챔버 내의 호기성 미생물에 산소를 공급하기 위한 산소 공급 용 밸브의 일실시예를 보인다.20 shows an embodiment of an oxygen supply valve for supplying oxygen to the aerobic microorganisms in the medium chamber.
이러한 산소공급용 밸브는 산소의 입출입을 허여하는 유공(10b), 상기 배지 챔버(132a 내지 132d)와 연결되는 연결통로(135) 및 마개(520a)로 구성된다.The oxygen supply valve is composed of a hole (10b) for allowing the entry and exit of oxygen, the connecting passage 135 and the stopper (520a) connected to the medium chambers (132a to 132d).
미생물 분석 장치 사용시, 몸체(100)는 고속으로 회전하게 되고, 이때 마개(520a)에 작용하는 강한 원심력에 의해 마개(520a)가 접착수단(521a)의 접착력(adhesive strength)을 이기고 이탈하면서 유공(10b)이 개방된다. When using the microbial analysis device, the body 100 is rotated at a high speed, at which time the stopper 520a beats the adhesive strength of the adhesive means 521a by the strong centrifugal force acting on the stopper 520a and leaves the pores ( 10b) is opened.
본 발명의 미생물 분석 장치에 있어서, 바람직하게는 상기 유공(10b)은 마개(520a)와 접착수단(521a)과의 접착력(또는 압착력)에 의해 폐쇄되고, 원심력에 의해 마개(520a)가 상기 접착수단(521a)과의 접착력(adhesive strength)을 이기고 이탈하면서 개방되는 것을 특징으로 한다.In the microorganism analyzing apparatus of the present invention, preferably, the hole 10b is closed by the adhesive force (or the pressing force) between the stopper 520a and the bonding means 521a, and the stopper 520a is attached by the centrifugal force. It is characterized in that it is opened while leaving the adhesive strength (adhesive strength) with the means 521a.
본 발명에서 상기 접착수단(521a)은 쿠션(cushion)이 있는 양면 테이프 혹은 고무재질의 코팅재료가 선호된다.  In the present invention, the adhesive means 521a is preferably a double-coated tape or a rubber coating material having a cushion.
상기 마개는 박막 원기둥의 고체가 선호된다. 이 경우 마개의 윗면만을 양면테이프(521a)로 유공(10b)에 밀착시켰다. The plug is preferably a solid thin film cylinder. In this case, only the upper surface of the stopper was brought into close contact with the hole 10b with a double-sided tape 521a.
또 다른 실시예로서, 유공(10b)의 폐쇄성을 증대시키기 위해 유공(10b) 부위를 쿠션(cushion) 있는 고무 내지 실리콘 재료로 사용하는 것이 선호된다.As another example, it is preferred to use the portion of the pores 10b as a cushioned rubber or silicone material to increase the closure of the pores 10b.
또 다른 실시예로서, 상기 마개는 박막 원기둥 모양의 영구자석을 사용하고 마개의 접착수단으로써 강자성체를 사용할 수 있다. 이 경우 유통기간 동안에는 마개와 강자성체의 인력에 의해 유공이 폐쇄되고, 사용시 몸체(100)의 고속 회전에 의해, 마개에 작용하는 강한 원심력에 의해 마개(520a)가 인력을 이기고 이탈하면서 유공(10b)이 개방된다. As another embodiment, the stopper may be a permanent magnet of a thin film cylindrical shape and a ferromagnetic material may be used as the stopper. In this case, during the distribution period, the hole is closed by the attraction force of the stopper and the ferromagnetic material, and the stopper 520a overcomes the attraction force and escapes due to the strong centrifugal force acting on the stopper by the high-speed rotation of the body 100 during use. Is opened.
도 21은 테스트 스트립(test strip)을 사용한 미생물분석 장치의 일 실시예이다. 21 is an embodiment of a microbial analysis apparatus using a test strip.
도면 부호 145는 멤브레인(membrane)을 사용한 테스트 스트립이다. Reference numeral 145 denotes a test strip using a membrane.
본 발명에서는 상기 테스트 스트립은 니트로셀룰로우즈 멤브레인(Nitrocellurose membrane) 내지 PVDF 멤브레인 상에 항체를 고정화하는 것이 선호되며, 이를 이하 테스트 라인이라 칭한다.In the present invention, the test strip is preferably immobilized with an antibody on a Nitrocellurose membrane or a PVDF membrane, which is referred to as a test line hereinafter.
상기 테스트 라인의 항체는 살모렐라, 리스테리아, O157균 중 선택된 하나 이상의 균과 특이적 결합을 하는 항체가 선호된다. The antibody of the test line is preferably an antibody that specifically binds to at least one selected from Salmonella, Listeria, and O157.
또한 상기 테스트 스트립은 샘플 패드(sample pad, 41a), 콘쥬게이트 패드(conjugate pad, 41b) 및 흡수 패드(absorbent pad, 41c)를 포함하는 것이 선호된다. 상기 콘쥬게이트 패드에는 골드 입자와 복합체를 이루는 골드 콘쥬게이트(gold conjugate)가 냉동건조된 형태로 침착된 것이 선호된다. The test strip also preferably includes a sample pad 41a, a conjugate pad 41b, and an absorbent pad 41c. The conjugate pad is preferably one in which a gold conjugate, which is complexed with gold particles, is deposited in a lyophilized form.
상기 테스트 스트립은 면역크로마토 그라피법에 의해 반응 검사를 하는 것을 특징으로 한다. 상기 면역크로마토 그라피법은 면역화학적 방법(Immunochemistry)과 크로마토 그라피법(Chromatogrphic Assay)을 결합한 검사방법으로 항원에 대한 항체의 특이적인 면역적반응성과 금입자(Colloidal gold)의 발색 특성과 다공성 멤브레인(Porous membrane)의 모세관현상에 의한 분자의 이동을 응용한 검사방법이다. The test strip is characterized in that the reaction test by immunochromatography. The immunochromatography method is a combination of immunochemistry and chromatography (Chromatogrphic Assay), the specific immunoreactivity of the antibody to the antigen, the color development characteristics of the colloidal gold and porous membrane (Porous) It is a test method that applies the movement of molecules by capillary phenomenon of membrane).
상기 테스트 스트립은 기준 라인(reference line)을 더 포함할 수 있으며, 기준 라인의 반응 농도는 음성 혹은 양성반응의 반별을 용이케 하기 위해 기준치(cutoff value)로 잡는 것을 특징으로 한다. The test strip may further include a reference line, and the reaction concentration of the reference line may be set to a cutoff value to facilitate the discrimination of negative or positive reactions.
기준 라인과 테스트 라인간의 반응강도(reaction intensity)의 차이 혹은 비(ratio)에 의해 정성 혹은 정량 분석하는 것을 특징으로 한다.Qualitative or quantitative analysis may be performed based on the difference or ratio of the reaction intensity between the reference line and the test line.
도 21은 검체를 주입하기 위한 검체 주입구(121); 상기 검체 주입구로부터 주입된 검체와 특이적 결합반응을 수행하기 위한 항체가 고정화되어 있는 프렙 챔버(130); 상기 프렙 챔버 내의 항체와 결합반응을 하지 않은 찌꺼기를 트레쉬 챔버(133)로 세척하여 보낸후, 상기 항체와 결합반응을 일으킨 미생물을 증균 챔버(131)로 보내기 위해, 파페인 다이제스쳔(papain digestion) 머캡토에탄올 환원(mecaptoethanol reduction) 또는 펩신 다이제스쳔(pepsin digestion)을 수행키 위한 시약을 포함하는 다이제스쳔 챔버(134); 상기 다이제스쳔에 의해 프렙 챔버(130)로부터 이송되어온 미생물을 증균하기 위한 액체 배양액(liquid culture medium)를 저장하고 있는 증균 챔버(131); 상기 증균된 미생물에 대해 항체 항원반응을 수행하기 위한 적어도 하나 이상의 항체가 고정화되어 있는 테스트 스트립(145)을 포함하는 스트립 챔버(132); 상기 스트립 챔버(132)로부터의 찌꺼기를 모으기 위한 트레쉬 챔버(133); 및 상기 챔버들간의 액체 이동을 제어하기 위한 밸브(64, 65, 66, 68); 상기 검체의 이동 경로를 제공하는 유로(채널); 및 상기 유로, 밸브 및 챔버가 집적화된 회전 가능한 몸체(100)를 포함하는 미생물 분석 장치의 일실시예를 보인다. 21 is a sample injection port 121 for injecting a sample; A prep chamber 130 in which an antibody is immobilized to perform a specific binding reaction with the sample injected from the sample injection port; After sending the waste which did not bind with the antibody in the prep chamber to the trech chamber 133, and sending the microorganism that caused the reaction with the antibody to the enrichment chamber 131, papain digest (papain). digestion digestion chamber 134 containing reagents for carrying out mecaptoethanol reduction or pepsin digestion; An enrichment chamber 131 for storing a liquid culture medium for enriching microorganisms transferred from the preparation chamber 130 by the digestion; A strip chamber 132 including a test strip 145 to which at least one antibody for immobilizing an antibody antigen reaction against the enriched microorganism is immobilized; A trace chamber 133 for collecting debris from the strip chamber 132; And valves (64, 65, 66, 68) for controlling liquid movement between the chambers; A flow path (channel) for providing a movement path of the specimen; And a rotatable body 100 in which the flow path, the valve and the chamber are integrated.
상기 다이제스쳔(pepsin digestion)을 수행하는 시약에 의해 항체는 절단되어 상기 프렙 챔버(130)로부터 이탈되어 증균 챔버(131)로 이송된다.The antibody is cleaved by the reagent that performs pepsin digestion, is separated from the preparation chamber 130, and transferred to the enrichment chamber 131.
본 발명의 또 다른 측면은, 상기 몸체(100)는 하나 이상의 스트립 챔버(132)을 포함할 수 있어, 서로 다른 미생물을 독립적으로 분석할 수 있도록 변형 될 수 있다.According to another aspect of the present invention, the body 100 may include one or more strip chambers 132, and may be modified to independently analyze different microorganisms.
본 발명의 또 다른 측면은, 상기 몸체(100)는 하나 이상의 스트립 챔버(132)와 프렙 챔버(130)을 포함할 수 있어, 서로 다른 미생물을 독립적으로 분석할 수 있도록 변형될 수 있다.In another aspect of the present invention, the body 100 may include one or more strip chamber 132 and the preparation chamber 130, it may be modified to independently analyze different microorganisms.
본 발명의 또 다른 측면은, 상기 프렙 챔버(130)는 자성체 비드(magnetric bead) 혹은 금 코팅된 마그네틱 비드를 포함하는 것을 특징으로 한다. 이 경우, 상기 항체가 마그네틱 비드의 표면에 고정화된다.In another aspect of the present invention, the preparation chamber 130 is characterized in that it comprises magnetic beads (magnetric bead) or gold coated magnetic beads. In this case, the antibody is immobilized on the surface of the magnetic beads.
상기 마그네틱 비드는 초상자성(superparamagnetic) 또는 강자성(ferromagnetic) 성질을 갖는 것이 선호된다. 또한, 프렙 챔버(130) 출구쪽 채널(130f)의 지름이 마그네틱 비드의 지름보다 작아 상기 마그네틱 비드가 프렙 챔버 내에 머무는 것이 선호된다. 본 발명에 있어서 상기 마그네틱 비드의 지름은 0.1um 내지 10um가 선호된다.The magnetic beads are preferably of superparamagnetic or ferromagnetic properties. It is also preferred that the diameter of the outlet channel 130f of the prep chamber 130 is smaller than the diameter of the magnetic bead so that the magnetic bead stays in the prep chamber. In the present invention, the diameter of the magnetic beads is preferably 0.1um to 10um.
상기 이동 가능한 영구자석(5b)을 의해 상기 이미지 센서(103)가 스트립 챔버(132)에 대한 이미지를 촬영하기 전에 "스트림 챔버의 탐색 과정"을 진행하는 것을 특징으로 한다. The movable sensor 5b is characterized in that the image sensor 103 performs a "search process of the stream chamber" before the image of the strip chamber 132 is taken.
본 발명의 미생물 분석 장치에 있어서, 바람직하게는 상기 이미지 센서 (103)와 스트립 챔버(132)간의 광학적 정렬(optical alignment)을 용이케 하기 위해 몸체(100)상에 영구자석(5c)을 구비한다.In the microbial analysis apparatus of the present invention, a permanent magnet 5c is preferably provided on the body 100 to facilitate optical alignment between the image sensor 103 and the strip chamber 132. .
영구자석(5c)과 이동 가능한 자석(5b)이 서로 만나면 두 자석간의 인력에 의해 몸체(100)는 더 이상 회전되지 않고, 상기 이미지 센서(103)와 스트립 챔버(132)간의 광학적 정렬(optical alignment)이 이루어 짐으로써 "스트림 챔버의 탐색 과정"이 완료된다. When the permanent magnet 5c and the movable magnet 5b meet each other, the body 100 is no longer rotated by the attraction between the two magnets, and an optical alignment between the image sensor 103 and the strip chamber 132 is achieved. ) Is completed, the "search process of the stream chamber" is completed.
도면 부호 170은 디스크 공극을 나타낸다. 도면 부호 335는 몸체의 기준을 나타내는 기준구멍이다. 도면 부호 188는 상기의 메모리 내장형 무선 RF IC 혹은 전자태그(tag)장치이다. Reference numeral 170 denotes a disk gap. Reference numeral 335 denotes a reference hole indicating a reference of the body. Reference numeral 188 denotes the above-described memory embedded wireless RF IC or electronic tag device.
본 발명은 또 다른 측면은 상기의 스트립 챔버 내에 테스트 스트립 대신, 고체 배지 내지 아가 배지가 사용될 수 있다. 이 경우 프렙 챔버(130) 내의 항체와 특이적 결합한 미생물만이 증균 챔버(131)에 이송되고, 이후 고체 배지 내지 아가 배지 상에서 배양되므로 분리배양의 신뢰도는 크게 향상된다. In another aspect of the present invention, a solid medium or agar medium may be used instead of the test strip in the strip chamber. In this case, only the microorganisms specifically bound to the antibody in the preparation chamber 130 is transferred to the enrichment chamber 131, and then cultured on the solid medium or agar medium, so that the reliability of separation culture is greatly improved.
따라서, 도 21은 미생물과 특이적 결합을 이룬 항체만 증균 챔버(131)내에서 배양되므로 미생물에 대한 선택도를 크게 증가시킬 수 있어, 면역학적 동정(identification)검사를 일괄적으로 수행할 수 있는 장점을 제공하는 일 실시예를 보인다. Therefore, FIG. 21 shows that only antibodies having a specific binding to the microorganisms are cultured in the enrichment chamber 131, thereby greatly increasing the selectivity for the microorganisms, and thus performing immunological identification tests collectively. One embodiment is provided that provides advantages.
이상, 여기에서는 본 발명을 특정 실시예에 관련하여 도시하고 설명하였지만, 본 발명이 그에 한정되는 것은 아니며, 이하의 특허청구의 범위는 본 발명의 정신과 분야를 이탈하지 않는 한도 내에서 본 발명이 다양하게 개조 및 변형될 수 있다는 것을 당 업계에서 통상의 지식을 가진 자가 용이하게 알 수 있다.As mentioned above, although the present invention has been illustrated and described with reference to specific embodiments, the present invention is not limited thereto, and the scope of the following claims is not limited to the scope of the present invention. It can be easily understood by those skilled in the art that can be modified and modified.
본 발명의 미생물 분석 장치 및 이를 이용한 분석 방법은 식중독균검사, 식품 미생물 검사, 수질 등의 오염 물질 검사를 위한 검체 내에 존재하는 소량의 미생물을 진단 및 탐지하는 박막형 장치에 적합하다. The microorganism analyzing apparatus of the present invention and an analysis method using the same are suitable for a thin film type apparatus for diagnosing and detecting a small amount of microorganisms present in a sample for contaminants such as food poisoning test, food microbial test, and water quality.

Claims (29)

  1. 검체를 주입하기 위한 하나 이상의 검체 주입구; One or more sample inlets for injecting a sample;
    상기 검체 주입구로부터 주입된 검체를 저장하기 위한 하나 이상의 프렙 챔버; One or more prep chambers for storing the sample injected from the sample inlet;
    상기 프렙 챔버에 저장된 검체 내의 미생물에 대해 영양분을 제공하기 위한 배지(culture medium)를 제공하는 하나 이상의 배지 챔버; One or more medium chambers providing a culture medium for providing nutrients to microorganisms in a sample stored in the preparation chamber;
    찌꺼기 및 과잉 검체를 모으기 위한 트레쉬 챔버;A trech chamber for collecting debris and excess sample;
    상기 프렙 챔버와 상기 배지 챔버, 상기 배지 챔버와 상기 트레쉬 챔버 사이의 검체의 이동 경로를 제공하는 복수의 채널; 및A plurality of channels providing a movement path of a sample between the preparation chamber and the medium chamber and the medium chamber and the trech chamber; And
    상기 챔버들 사이에서 유체 및 검체를 이동시킬 있도록 구성되는 하나 이상의 밸브를 포함하는 회전 가능한 몸체를 구비하는 것을 특징으로 하는, A rotatable body comprising one or more valves configured to move fluid and sample between the chambers,
    미생물 분석 장치.Microbial Analysis Device.
  2. 제1항에 있어서, The method of claim 1,
    상기 미생물 분석 장치는,The microbial analysis device,
    검사 부위로부터 검체를 수집하거나 수집된 검체를 상기 검체 주입구에 주입하기 위한 샘플러(sampler)를 더 포함하는 것을 특징으로 하는, Characterized in that it further comprises a sampler for collecting a sample from the test site or injecting the collected sample into the sample inlet,
    미생물 분석 장치.Microbial Analysis Device.
  3. 제2항에 있어서, The method of claim 2,
    상기 샘플러는 수집된 검체로부터 미생물을 추출하기 위한 미생물 추출액을 포함하거나 또는 검체에 존재하는 미생물을 증균하기 위한 액체 배양액(liquid culture medium)을 포함하는 것을 특징으로 하는, The sampler may include a microbial extract for extracting the microorganisms from the collected sample or a liquid culture medium for enriching the microorganisms present in the sample.
    미생물 분석 장치.Microbial Analysis Device.
  4. 제2항에 있어서, The method of claim 2,
    상기 샘플러는 피펫, 디스펜서(dispensor), 피펫, 주사위, 란셋(lancet) 중 어느 하나인 것을 특징으로 하는,The sampler may be any one of a pipette, a dispenser, a pipette, a dice, a lancet,
    미생물 분석 장치.Microbial Analysis Device.
  5. 제1항에 있어서, The method of claim 1,
    상기 미생물 분석 장치는, The microbial analysis device,
    상기 프렙 챔버로부터의 검체를 취하여 상기 검체 내에 존재하는 미생물을 증균하기 위한 액체 배양액을 포함하는 증균 챔버를 더 포함하는 것을 특징으로 하는, Further comprising a enrichment chamber containing a liquid culture for enriching the microorganisms present in the sample by taking a sample from the prep chamber,
    미생물 분석 장치.Microbial Analysis Device.
  6. 제3항 또는 제5항에 있어서, The method according to claim 3 or 5,
    상기 액체 배양액은 mEC Broth, RV(Rappaport Vassiliadis) Broth, Listeria Enrichment Broth, UVM-modified Listeria Enrichment Broth, Fraser Broth, TSB(Tryptone Sonya Broth), PBS (phosphate buffered saline), Peptone water, lactose Broth, desoxycholate lactose Broth 중 선택된 어느 하나인 것을 특징으로 하는, The liquid culture medium is mEC Broth, Rappaport Vassiliadis (RV) Broth, Listeria Enrichment Broth, UVM-modified Listeria Enrichment Broth, Fraser Broth, Tryptone Sonya Broth (TSB), PBS (phosphate buffered saline), Peptone water, lactose Broth, desoxycholate lactose Characterized in that any one selected from Broth,
    미생물 분석 장치.Microbial Analysis Device.
  7. 제1항에 있어서, The method of claim 1,
    상기 배지는 XLD Agar, MacConkey Agar, Desoxycholate, Citrate Agar, Bismuth Sulfite Agar, Nutrient Agar, TSI Agar, Sorbitol MacConkey Agar, Oxford Agar, PALCAM Agar, LPM Agar, Mannitol Salt Agar, Baird-Parker Agar, MYP Agar, 크로모제닉 배지(Chromogenic medium), 고체 배지, 플로로제닉 배지(fluorogenic medium), 감별 배지(Differential medium), 선택 배지(selective medium)중 선택된 적어도 어느 하나 이상을 포함하는 것을 특징으로 하는, The medium is XLD Agar, MacConkey Agar, Desoxycholate, Citrate Agar, Bismuth Sulfite Agar, Nutrient Agar, TSI Agar, Sorbitol MacConkey Agar, Oxford Agar, PALCAM Agar, LPM Agar, Mannitol Salt Agar, Baird-Parker Agar, MYP Agar, Croissant Characterized in that it comprises at least one selected from a chromogenic medium, a solid medium, a fluorogenic medium, a differential medium, a selective medium,
    미생물 분석 장치.Microbial Analysis Device.
  8. 제1항에 있어서,The method of claim 1,
    상기 미생물 분석 장치는, The microbial analysis device,
    동정 실험(identification test)을 허여하는 개폐가 가능한 커버 수단을 더 포함하는 것을 특징으로 하는, Further comprising a cover means capable of opening and closing allowing an identification test,
    미생물 분석 장치.Microbial Analysis Device.
  9. 제8항에 있어서, The method of claim 8,
    상기 커버 수단은 자성체 시트(magnetic sheet) 와 영구 자석으로 구성된 것을 특징으로 하는, The cover means, characterized in that consisting of a magnetic sheet (magnetic sheet) and a permanent magnet,
    미생물 분석 장치.Microbial Analysis Device.
  10. 제1항에 있어서, The method of claim 1,
    상기 미생물 분석 장치는, The microbial analysis device,
    호기성 미생물에 산소를 공급하기 위한 산소 공급용 밸브를 더 포함하는 것을 특징으로 하는, Further comprising a oxygen supply valve for supplying oxygen to the aerobic microorganism,
    미생물 분석 장치.Microbial Analysis Device.
  11. 제1항에 있어서, The method of claim 1,
    상기 미생물 분석 장치는, The microbial analysis device,
    미생물을 관찰할 수 있는 현미경 센서; 및Microscope sensors capable of observing microorganisms; And
    상기 현미경 센서로부터의 이미지를 처리하고 콜로니를 계수하기 위한 중앙제어장치를 더 포함하는 것을 특징으로 하는, And further comprising a central controller for processing images from the microscope sensor and counting colonies.
    미생물 분석 장치.Microbial Analysis Device.
  12. 제1항에 있어서, The method of claim 1,
    상기 미생물 분석 장치는, The microbial analysis device,
    상기 미생물 증균을 위한 온도 제어 장치 또는 항온기를 더 포함하는 것을 특징으로 하는, Further comprising a temperature control device or a thermostat for the microbial enrichment,
    미생물 분석 장치.Microbial Analysis Device.
  13. 제1항에 있어서, The method of claim 1,
    상기 밸브는 "수압 버스트 밸브", "마개 버스트 밸브", "자석 버스트 밸브, "레이저 버스트 밸브" 및 "소수성 버스트 밸브" 중 어느 하나 이상인 것을 특징으로 하는,The valve is characterized in that any one or more of "hydraulic burst valve", "plug burst valve", "magnetic burst valve," laser burst valve "and" hydrophobic burst valve ",
    미생물 분석 장치.Microbial Analysis Device.
  14. 제1항에 있어서, The method of claim 1,
    상기 미생물 분석 장치는 상기 몸체 상에 RF IC를 더 포함하는 것을 특징으로 하는,The microbial analysis device further comprises an RF IC on the body,
    미생물 분석 장치.Microbial Analysis Device.
  15. 제13항에 있어서, The method of claim 13,
    상기 밸브의 개폐는 레이저빔 발생 장치에 의해 발생되는 열에 의해 추가적으로 수행되는 것을 특징으로 하는, The opening and closing of the valve is characterized in that additionally performed by the heat generated by the laser beam generating device,
    미생물 분석 장치.Microbial Analysis Device.
  16. 제1항에 있어서, The method of claim 1,
    상기 미생물 분석 장치는, The microbial analysis device,
    상기 배지 챔버의 미생물을 검출하기 위한 이미지 센싱 장치; 및An image sensing device for detecting microorganisms in the medium chamber; And
    상기 디스크를 회전시키기 위한 스핀들 모터를 더 포함하는 것을 특징으로 하는, Further comprising a spindle motor for rotating the disk,
    미생물 분석 장치.Microbial Analysis Device.
  17. 제16항에 있어서, The method of claim 16,
    상기 이미지 센싱 장치는, The image sensing device,
    상기 배지 챔버 내의 미생물 콜로니를 계수하여 정량분석하는 것을 특징으로 하는, Characterized by quantifying the microbial colony in the medium chamber,
    미생물 분석 장치.Microbial Analysis Device.
  18. 제16항에 있어서, The method of claim 16,
    상기 이미지 센싱 장치는, The image sensing device,
    상기 몸체 상에 표시된 바코드를 판독하는 것을 특징으로 하는, Characterized in that to read the barcode displayed on the body,
    미생물 분석 장치.Microbial Analysis Device.
  19. 제18항에 있어서, The method of claim 18,
    상기 바코드에는 상기 몸체의 제품 ID, 유효 기간, 분석 및 진단할 수 있는 미생물의 종류 중 어느 하나 이상의 정보가 포함되어 있는 것을 특징으로 하는,The bar code includes any one or more of the product ID of the body, the expiration date, the type of microorganism that can be analyzed and diagnosed,
    미생물 분석 장치.Microbial Analysis Device.
  20. 제1항에 있어서, The method of claim 1,
    상기 미생물 분석 장치는, The microbial analysis device,
    미생물 분석 결과를 표시해주거나 상기 미생물 분석 장치의 주요 공정에 따른 진행 상태를 표시하는 표시 장치를 더 포함하는 것을 특징으로 하는, Further comprising a display device for displaying the results of the microbial analysis or display the progress state according to the main process of the microbial analysis device,
    미생물 분석 장치.Microbial Analysis Device.
  21. 검체를 주입하기 위한 하나 이상의 검체 주입구; One or more sample inlets for injecting a sample;
    상기 검체 주입구로부터 주입된 검체와 특이적 결합반응을 수행하기 위한 항체가 고정화되어 있는 하나 이상의 프렙 챔버; At least one prep chamber in which an antibody is immobilized to perform a specific binding reaction with a sample injected from the sample injection port;
    상기 항체와 결합반응을 일으킨 미생물을 증균하기 위한 액체 배양액을 저장하고 있는 하나 이상의 증균 챔버;One or more enrichment chambers for storing a liquid culture medium for enriching the microorganism that caused the reaction with the antibody;
    찌꺼기 및 과잉 검체를 모으기 위한 트레쉬 챔버;A trech chamber for collecting debris and excess sample;
    상기 증균 챔버에서 증균된 미생물에 대해 항체 항원반응을 수행하기 위한 하나 이상의 항체가 고정화되어 있는 테스트 스트립을 포함하는 스트립 챔버;A strip chamber comprising a test strip on which at least one antibody is immobilized to perform antibody antigen reaction against the microorganism enriched in the enrichment chamber;
    상기 프렙 챔버, 증균 챔버, 트레쉬 챔버 및 스트립 챔버 사이의 검체의 이동 경로를 제공하는 복수의 채널; 및A plurality of channels providing a path of movement of the sample between the prep chamber, enrichment chamber, trech chamber and strip chamber; And
    상기 챔버들 사이에서 유체 및 검체를 이동시킬 있도록 구성되는 하나 이상의 밸브를 포함하는 회전 가능한 몸체를 구비하는 것을 특징으로 하는, A rotatable body comprising one or more valves configured to move fluid and sample between the chambers,
    미생물 분석 장치.Microbial Analysis Device.
  22. 검체를 주입하기 위한 하나 이상의 검체 주입구; One or more sample inlets for injecting a sample;
    상기 검체 주입구로부터 주입된 검체와 특이적 결합반응을 수행하기 위한 항체가 고정화되어 있는 하나 이상의 프렙 챔버; At least one prep chamber in which an antibody is immobilized to perform a specific binding reaction with a sample injected from the sample injection port;
    상기 항체와 결합반응을 일으킨 미생물을 증균하기 위한 액체 배양액을 저장하고 있는 하나 이상의 증균 챔버;One or more enrichment chambers for storing a liquid culture medium for enriching the microorganism that caused the reaction with the antibody;
    찌꺼기 및 과잉 검체를 모으기 위한 트레쉬 챔버;A trech chamber for collecting debris and excess sample;
    상기 증균 챔버에서 증균된 미생물에 영양분을 제공하기 위한 배지를 제공하는 하나 이상의 배지 챔버; At least one medium chamber providing a medium for providing nutrients to the microorganisms enriched in the enrichment chamber;
    상기 프렙 챔버, 증균 챔버, 배지 챔버 및 스트립 챔버 사이의 검체의 이동 경로를 제공하는 복수의 채널; 및A plurality of channels providing a path of movement of the sample between the preparation chamber, enrichment chamber, media chamber and strip chamber; And
    상기 챔버들 사이에서 유체 및 검체를 이동시킬 있도록 구성되는 하나 이상의 밸브를 포함하는 회전 가능한 몸체를 구비하는 것을 특징으로 하는, A rotatable body comprising one or more valves configured to move fluid and sample between the chambers,
    미생물 분석 장치.Microbial Analysis Device.
  23. 제21항 또는 제22항에 있어서, The method of claim 21 or 22,
    파페인 다이제스쳔(papain digestion), 머캡토에탄올 환원(mecaptoethanol reduction) 및 펩신 다이제스쳔(pepsin digestion)중 어느 하나 이상의 반응을 수행할 수 있는 시약을 포함하는 다이제스쳔 챔버를 더 포함하는 것을 특징으로 하는, And further comprising a digestion chamber containing reagents capable of carrying out the reaction of any one or more of papain digestion, mecaptoethanol reduction and pepsin digestion. Characterized by
    미생물 분석 장치.Microbial Analysis Device.
  24. 제21항 또는 제22항에 있어서, The method of claim 21 or 22,
    상기 항체는 살모렐라, 리스테리아, O157균 중에서 선택된 하나 이상의 균과 특이적 결합을 하는 항체인 것을 특징으로 하는, The antibody is characterized in that the antibody specifically binding to one or more bacteria selected from Salmonella, Listeria, O157,
    미생물 분석 장치.Microbial Analysis Device.
  25. (a) 프렙 챔버에 검체를 주입하는 단계;(a) injecting a sample into a prep chamber;
    (b) 상기 프렙 챔버 내의 검체를 배지 챔버로 이송하는 단계; (b) transferring the sample in the preparation chamber to the media chamber;
    (c) 상기 배지 챔버로 이송된 상기 검체를 가지고 상기 배지 챔버 내의 배지 표면에 도포 접종하는 단계;(c) applying and inoculating the surface of the medium in the medium chamber with the sample transferred to the medium chamber;
    (d) 도포 접종 후 잉여 검체를 트레쉬 챔버에 수용하는 단계; 및(d) receiving the surplus sample in the tresh chamber after application inoculation; And
    (e) 이미지 센싱 장치에 의해 상기 배지 챔버 내의 미생물을 판독하고 미생물 콜로니를 계수하는 단계를 포함하는 것을 특징으로 하는, (e) reading the microorganisms in the medium chamber and counting microbial colonies by the image sensing device,
    미생물 분석 방법. Microbiological Analysis Method.
  26. 제25항에 있어서, The method of claim 25,
    상기 (a) 단계는, In step (a),
    검사 부위로부터 검체를 수집하고 수집된 검체를 상기 검체 주입구에 주입하기 위한 샘플러(sampler)를 이용해 수행되는 것을 특징으로 하는, Collecting a sample from the inspection site and characterized in that carried out using a sampler (sampler) for injecting the collected sample into the sample inlet,
    미생물 분석 방법. Microbiological Analysis Method.
  27. 제25항에 있어서, The method of claim 25,
    상기 (a) 단계 및 상기 (b) 단계 사이에, Between step (a) and step (b),
    상기 프렙 챔버로부터의 검체를 취하여 상기 검체 내에 존재하는 미생물을 액체 배양액(liquid culture medium)에서 증균 및 배양하는 단계를 더 포함하는 것을 특징으로 하는,And taking a sample from the prep chamber and enriching and culturing the microorganism present in the sample in a liquid culture medium.
    미생물 분석 방법. Microbiological Analysis Method.
  28. 제25항에 있어서, The method of claim 25,
    상기 방법은, The method,
    (f) 상기 배지 챔버 판독 결과를 원격지 서버로 송신하는 단계를 더 포함하는 것을 특징으로 하는, (f) further transmitting the result of the discharge chamber reading to a remote server,
    미생물 분석 방법. Microbiological Analysis Method.
  29. 제28항에 있어서, The method of claim 28,
    상기 방법은, The method,
    (g) 상기 원격지 서버는 검사결과를 바탕으로 위생점수가 사용자의 표시장치 내지 상점간판(signboard)에 표시되도록 하는 단계를 더 포함하는 것을 특징으로 하는, (g) the remote server further comprises the step of causing the sanitary score to be displayed on the user's display device or signboard based on the inspection result;
    미생물 분석 방법.Microbiological Analysis Method.
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EP3660160A1 (en) * 2018-11-28 2020-06-03 GTZ Microlab Detect, S.L. Portable device and method for detecting microorganisms or metabolites in a sample
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FR2677664A1 (en) 1991-06-13 1992-12-18 Millipore Sa Device and process for the microbiological control of liquids under pressure
EP1064351A1 (en) * 1998-03-19 2001-01-03 Amanzi Technologies Limited Microbiological testing of a liquid sample
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CN107663504A (en) * 2017-10-09 2018-02-06 宜昌喜旺食品有限公司 A kind of equipment and detection method of Intelligent Measurement milk bacterial number
CN107663504B (en) * 2017-10-09 2024-03-19 宜昌喜旺食品有限公司 Device and method for intelligently detecting bacterial quantity of milk
EP3660160A1 (en) * 2018-11-28 2020-06-03 GTZ Microlab Detect, S.L. Portable device and method for detecting microorganisms or metabolites in a sample
WO2020109457A1 (en) * 2018-11-28 2020-06-04 Gtz Microlab Detect, S.L. Portable device and method for detecting microorganisms or metabolites in a sample
WO2023133714A1 (en) * 2022-01-12 2023-07-20 广州工商学院 Fungus quantity measurement device and measurement method for testing yogurt quality

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