WO2011033506A3 - Isolated pon1 polypeptides, polynucleotides encoding same and uses thereof in treating or preventing organophosphate exposure associated damage - Google Patents

Isolated pon1 polypeptides, polynucleotides encoding same and uses thereof in treating or preventing organophosphate exposure associated damage Download PDF

Info

Publication number
WO2011033506A3
WO2011033506A3 PCT/IL2010/000754 IL2010000754W WO2011033506A3 WO 2011033506 A3 WO2011033506 A3 WO 2011033506A3 IL 2010000754 W IL2010000754 W IL 2010000754W WO 2011033506 A3 WO2011033506 A3 WO 2011033506A3
Authority
WO
WIPO (PCT)
Prior art keywords
pon1
isolated
polypeptides
treating
polynucleotides encoding
Prior art date
Application number
PCT/IL2010/000754
Other languages
French (fr)
Other versions
WO2011033506A2 (en
WO2011033506A9 (en
Inventor
Dan S. Tawfik
Rinkoo Devi Gupta
Moshe Goldsmith
Yaacov Ashani
Moshe Ben-David
Israel Silman
Joel L. Sussman
Haim Leader
Original Assignee
Yeda Research And Development Co. Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yeda Research And Development Co. Ltd. filed Critical Yeda Research And Development Co. Ltd.
Priority to EP10768078A priority Critical patent/EP2478093A2/en
Publication of WO2011033506A2 publication Critical patent/WO2011033506A2/en
Publication of WO2011033506A9 publication Critical patent/WO2011033506A9/en
Publication of WO2011033506A3 publication Critical patent/WO2011033506A3/en
Priority to IL218613A priority patent/IL218613A0/en
Priority to US13/420,920 priority patent/US8735124B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)

Abstract

An isolated polypeptide comprising the amino acid sequence of serum paraoxonase (PON1) having catalytic efficiency of k cat /K M ≈106 M-1min-1 for a nerve-agent substrate.
PCT/IL2010/000754 2009-09-17 2010-09-15 Isolated pon1 polypeptides, polynucleotides encoding same and uses thereof in treating or preventing organophosphate exposure associated damage WO2011033506A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP10768078A EP2478093A2 (en) 2009-09-17 2010-09-15 Isolated pon1 polypeptides, polynucleotides encoding same and uses thereof in treating or preventing organophosphate exposure associated damage
IL218613A IL218613A0 (en) 2009-09-17 2012-03-13 Isolated pon1 polypeptides, polynucleotides encoding same and uses thereof in treating or preventing organophosphate exposure associated damage
US13/420,920 US8735124B2 (en) 2009-09-17 2012-03-15 Isolated PON1 polypeptides, polynucleotides encoding same and uses thereof in treating or preventing organophosphate exposure associated damage

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US27236309P 2009-09-17 2009-09-17
US61/272,363 2009-09-17

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US13/420,920 Continuation-In-Part US8735124B2 (en) 2009-09-17 2012-03-15 Isolated PON1 polypeptides, polynucleotides encoding same and uses thereof in treating or preventing organophosphate exposure associated damage

Publications (3)

Publication Number Publication Date
WO2011033506A2 WO2011033506A2 (en) 2011-03-24
WO2011033506A9 WO2011033506A9 (en) 2011-05-05
WO2011033506A3 true WO2011033506A3 (en) 2011-09-29

Family

ID=43589971

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IL2010/000754 WO2011033506A2 (en) 2009-09-17 2010-09-15 Isolated pon1 polypeptides, polynucleotides encoding same and uses thereof in treating or preventing organophosphate exposure associated damage

Country Status (2)

Country Link
EP (1) EP2478093A2 (en)
WO (1) WO2011033506A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8735124B2 (en) * 2009-09-17 2014-05-27 Yeda Research And Development Co. Ltd. Isolated PON1 polypeptides, polynucleotides encoding same and uses thereof in treating or preventing organophosphate exposure associated damage
WO2013040501A1 (en) 2011-09-16 2013-03-21 Pharmathene, Inc. Compositions and combinations of organophosphorus bioscavengers and hyaluronan-degrading enzymes, and uses thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004078991A2 (en) * 2003-03-04 2004-09-16 Yeda Research And Development Co. Ltd. Pon polypeptides, polynucleotides encoding same and compositions and methods utilizing same
WO2007105223A2 (en) * 2006-03-16 2007-09-20 Yeda Research And Development Co. Ltd. Methods of diagnosing pon1-hdl associated lipid disorders

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL154600B (en) 1971-02-10 1977-09-15 Organon Nv METHOD FOR THE DETERMINATION AND DETERMINATION OF SPECIFIC BINDING PROTEINS AND THEIR CORRESPONDING BINDABLE SUBSTANCES.
NL154598B (en) 1970-11-10 1977-09-15 Organon Nv PROCEDURE FOR DETERMINING AND DETERMINING LOW MOLECULAR COMPOUNDS AND PROTEINS THAT CAN SPECIFICALLY BIND THESE COMPOUNDS AND TEST PACKAGING.
NL154599B (en) 1970-12-28 1977-09-15 Organon Nv PROCEDURE FOR DETERMINING AND DETERMINING SPECIFIC BINDING PROTEINS AND THEIR CORRESPONDING BINDABLE SUBSTANCES, AND TEST PACKAGING.
US3901654A (en) 1971-06-21 1975-08-26 Biological Developments Receptor assays of biologically active compounds employing biologically specific receptors
US3853987A (en) 1971-09-01 1974-12-10 W Dreyer Immunological reagent and radioimmuno assay
US3867517A (en) 1971-12-21 1975-02-18 Abbott Lab Direct radioimmunoassay for antigens and their antibodies
NL171930C (en) 1972-05-11 1983-06-01 Akzo Nv METHOD FOR DETERMINING AND DETERMINING BITES AND TEST PACKAGING.
US3850578A (en) 1973-03-12 1974-11-26 H Mcconnell Process for assaying for biologically active molecules
US3935074A (en) 1973-12-17 1976-01-27 Syva Company Antibody steric hindrance immunoassay with two antibodies
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4034074A (en) 1974-09-19 1977-07-05 The Board Of Trustees Of Leland Stanford Junior University Universal reagent 2-site immunoradiometric assay using labelled anti (IgG)
US3984533A (en) 1975-11-13 1976-10-05 General Electric Company Electrophoretic method of detecting antigen-antibody reaction
US4098876A (en) 1976-10-26 1978-07-04 Corning Glass Works Reverse sandwich immunoassay
US4879219A (en) 1980-09-19 1989-11-07 General Hospital Corporation Immunoassay utilizing monoclonal high affinity IgM antibodies
US5011771A (en) 1984-04-12 1991-04-30 The General Hospital Corporation Multiepitopic immunometric assay
US4666828A (en) 1984-08-15 1987-05-19 The General Hospital Corporation Test for Huntington's disease
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4801531A (en) 1985-04-17 1989-01-31 Biotechnology Research Partners, Ltd. Apo AI/CIII genomic polymorphisms predictive of atherosclerosis
US5272057A (en) 1988-10-14 1993-12-21 Georgetown University Method of detecting a predisposition to cancer by the use of restriction fragment length polymorphism of the gene for human poly (ADP-ribose) polymerase
US5192659A (en) 1989-08-25 1993-03-09 Genetype Ag Intron sequence analysis method for detection of adjacent and remote locus alleles as haplotypes
US5281521A (en) 1992-07-20 1994-01-25 The Trustees Of The University Of Pennsylvania Modified avidin-biotin technique
WO2000064539A1 (en) 1999-04-26 2000-11-02 U.S. Army Medical Research And Materiel Command Method to make op detoxifying sponges
US20040109853A1 (en) 2002-09-09 2004-06-10 Reactive Surfaces, Ltd. Biological active coating components, coatings, and coated surfaces

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004078991A2 (en) * 2003-03-04 2004-09-16 Yeda Research And Development Co. Ltd. Pon polypeptides, polynucleotides encoding same and compositions and methods utilizing same
WO2007105223A2 (en) * 2006-03-16 2007-09-20 Yeda Research And Development Co. Ltd. Methods of diagnosing pon1-hdl associated lipid disorders

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
AHARONI AMIR ET AL: "Directed evolution of mammalian paraoxonases PON1 and PON3 for bacterial expression and catalytic specialization", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES (PNAS), NATIONAL ACADEMY OF SCIENCE, US, vol. 101, no. 2, 13 January 2004 (2004-01-13), pages 482 - 487, XP002438727, ISSN: 0027-8424, DOI: DOI:10.1073/PNAS.2536901100 *
ASHANI Y ET AL: "Stereo-specific synthesis of analogs of nerve agents and their utilization for selection and characterization of paraoxonase (PON1) catalytic scavengers", CHEMICO-BIOLOGICAL INTERACTIONS, ELSEVIER SCIENCE IRLAND, IR, vol. 187, no. 1-3, 6 September 2010 (2010-09-06), pages 362 - 369, XP027174373, ISSN: 0009-2797, [retrieved on 20100319] *
BAJGAR JIRÍ: "Organophosphates/nerve agent poisoning: mechanism of action, diagnosis, prophylaxis, and treatment", ADVANCES IN CLINICAL CHEMISTRY, ACADEMIC PRESS, LONDON, GB, vol. 38, 1 January 2004 (2004-01-01), pages 151 - 216, XP009109278, ISSN: 0065-2423, DOI: DOI:10.1016/S0065-2423(04)38006-6 *
GUPTA RINKOO D ET AL: "Directed evolution of hydrolases for prevention of G-type nerve agent intoxication.", NATURE CHEMICAL BIOLOGY FEB 2011 LNKD- PUBMED:21217689, vol. 7, no. 2, February 2011 (2011-02-01), pages 120 - 125, XP002627100, ISSN: 1552-4469 *
HAREL M ET AL: "Structure and evolution of the serum paraoxonase family of detoxifying and antiatherosclerotic enzymes", NATURE STRUCTURAL & MOLECULAR BIOLOGY NATURE PUBLISHING GROUP USA, vol. 11, no. 5, May 2004 (2004-05-01), pages 412 - 419, XP002627099, ISSN: 1545-9993 *
ROCHU ET AL: "Human paraoxonase: A promising approach for pre-treatment and therapy of organophosphorus poisoning", TOXICOLOGY, LIMERICK, IR, vol. 233, no. 1-3, 31 March 2007 (2007-03-31), pages 47 - 59, XP022012211, ISSN: 0300-483X, DOI: DOI:10.1016/J.TOX.2006.08.037 *

Also Published As

Publication number Publication date
WO2011033506A2 (en) 2011-03-24
WO2011033506A9 (en) 2011-05-05
EP2478093A2 (en) 2012-07-25

Similar Documents

Publication Publication Date Title
PH12015500528A1 (en) Fc containing polypeptides with altered glycosylation and reduced
WO2007035600A3 (en) Natriuretic activities
WO2006103666A3 (en) Isolated bid polypeptides, polynucleotides encoding same and antibodies directed thereagainst and methods of using same for inducing cell cycle arrest or apoptosis
WO2009042237A3 (en) Methods and compositions for modulating bcl-2 family polypeptides
NZ591855A (en) Novel immunotherapy against several tumors including neuronal and brain tumors
JP2013510581A5 (en)
EA201170878A1 (en) POLYPEPTIDES WITH XYLANASE ACTIVITY
WO2008070082A3 (en) Stem-progenitor cell specific micro-ribonucleic acids and uses thereof
WO2006066024A8 (en) Pancreatic polypeptide family motifs, polypeptides and methods comprising the same
WO2007148224A3 (en) Polypeptide
WO2013052946A3 (en) Genetically encoded biosensors
ATE453664T1 (en) HOUSE DUST MITE ALLERGEN
WO2010031988A3 (en) Polypeptides substrates of lrrk2 and uses thereof
WO2011056954A3 (en) Haemophilus parasuis polypeptides and methods of use
WO2011033506A3 (en) Isolated pon1 polypeptides, polynucleotides encoding same and uses thereof in treating or preventing organophosphate exposure associated damage
WO2011123858A3 (en) Ccn3 peptides and analogs thereof for therapeutic use
WO2016092555A3 (en) Isolated phosphotriesterase polypeptides, polynucleotides encoding same and uses thereof in treating or preventing organophosphate exposure associated damage
WO2008148932A3 (en) Glycosylasparaginase for treatment of cancers or inflammatory diseases
WO2008155363A3 (en) Identification of new isoforms of the mhc-class i specific receptor cd160 and uses thereof
WO2006123157A3 (en) Nematistatic protein
WO2004041859A3 (en) Peptide fragments of the harp factor inhibiting angiogenesis
WO2008109433A8 (en) Anti-angiogenic peptides
WO2007073845A3 (en) Polypeptide having esterase activity and recombinant esterase and use thereof
WO2012035322A3 (en) A photocatalytic body
WO2006116789A3 (en) Permeable capsules

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 10768078

Country of ref document: EP

Kind code of ref document: A1

DPE1 Request for preliminary examination filed after expiration of 19th month from priority date (pct application filed from 20040101)
WWE Wipo information: entry into national phase

Ref document number: 218613

Country of ref document: IL

NENP Non-entry into the national phase

Ref country code: DE

REEP Request for entry into the european phase

Ref document number: 2010768078

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2010768078

Country of ref document: EP