WO2011028296A3 - Sequence determination by use of opposing forces - Google Patents

Sequence determination by use of opposing forces Download PDF

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Publication number
WO2011028296A3
WO2011028296A3 PCT/US2010/002451 US2010002451W WO2011028296A3 WO 2011028296 A3 WO2011028296 A3 WO 2011028296A3 US 2010002451 W US2010002451 W US 2010002451W WO 2011028296 A3 WO2011028296 A3 WO 2011028296A3
Authority
WO
WIPO (PCT)
Prior art keywords
various embodiments
nucleic
opposing forces
sequencing
systems
Prior art date
Application number
PCT/US2010/002451
Other languages
French (fr)
Other versions
WO2011028296A9 (en
WO2011028296A2 (en
Inventor
Javier Farinas
Andrea Chow
John Wallace Parce
Original Assignee
Caerus Molecular Diagnostics Incorporated
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Caerus Molecular Diagnostics Incorporated filed Critical Caerus Molecular Diagnostics Incorporated
Priority to EP10814098A priority Critical patent/EP2475787A2/en
Publication of WO2011028296A2 publication Critical patent/WO2011028296A2/en
Publication of WO2011028296A9 publication Critical patent/WO2011028296A9/en
Publication of WO2011028296A3 publication Critical patent/WO2011028296A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6872—Methods for sequencing involving mass spectrometry
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

Abstract

The present teachings relate to systems, methods, and the like, for analyzing biological polymers, by use of opposing forces. Among other things, the present teachings can be used to determine sequence information, such as in genetic sequencing and genotyping applications. Various embodiments are described for efficient, high throughput sequencing of nucleic-acid molecules, such as DNA. Various embodiments are described wherein nucleic-acid sequence information is determined without the need or use of extrinsic labels. As well various embodiments of methods, systems, and the like, are described, which can provide long and accurate read lengths for low-cost nucleic acid sequencing.
PCT/US2010/002451 2009-09-07 2010-09-07 Sequence determination by use of opposing forces WO2011028296A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP10814098A EP2475787A2 (en) 2009-09-07 2010-09-07 Sequence determination by use of opposing forces

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US24030409P 2009-09-07 2009-09-07
US61/240,304 2009-09-07

Publications (3)

Publication Number Publication Date
WO2011028296A2 WO2011028296A2 (en) 2011-03-10
WO2011028296A9 WO2011028296A9 (en) 2011-07-07
WO2011028296A3 true WO2011028296A3 (en) 2011-08-25

Family

ID=43648217

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2010/002451 WO2011028296A2 (en) 2009-09-07 2010-09-07 Sequence determination by use of opposing forces

Country Status (3)

Country Link
US (1) US20110059864A1 (en)
EP (1) EP2475787A2 (en)
WO (1) WO2011028296A2 (en)

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US20140216935A1 (en) * 2012-08-08 2014-08-07 Lehigh University Dielectrophoretic Tweezers as a Platform for Molecular Force Spectroscopy in a Highly Parallel Format
DE102013215666A1 (en) * 2013-08-08 2015-02-12 Siemens Aktiengesellschaft Method for sequencing biopolymers
US11046995B2 (en) * 2016-08-16 2021-06-29 The Regents Of The University Of California Method for finding low abundance sequences by hybridization (FLASH)
NL2019937B1 (en) * 2017-11-17 2019-05-24 Stichting Vu Method and system for optical force measurement
CN114859076B (en) * 2022-07-06 2022-10-21 之江实验室 Acceleration measurement method and device based on optical suspension multi-microsphere array

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Also Published As

Publication number Publication date
US20110059864A1 (en) 2011-03-10
WO2011028296A9 (en) 2011-07-07
WO2011028296A2 (en) 2011-03-10
EP2475787A2 (en) 2012-07-18

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