WO2011000498A1 - Methods and means for obtaining plants with enhanced glyphosate tolerance - Google Patents

Methods and means for obtaining plants with enhanced glyphosate tolerance Download PDF

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Publication number
WO2011000498A1
WO2011000498A1 PCT/EP2010/003797 EP2010003797W WO2011000498A1 WO 2011000498 A1 WO2011000498 A1 WO 2011000498A1 EP 2010003797 W EP2010003797 W EP 2010003797W WO 2011000498 A1 WO2011000498 A1 WO 2011000498A1
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WO
WIPO (PCT)
Prior art keywords
plant
glyphosate
dna
gene
encoding
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2010/003797
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English (en)
French (fr)
Inventor
Rene Ruiter
Frank Meulewaeter
Chantal Vanderstraeten
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer CropScience NV
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Bayer Bioscience NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to JP2012518040A priority Critical patent/JP2012531216A/ja
Priority to EA201270107A priority patent/EA201270107A1/ru
Priority to AU2010268400A priority patent/AU2010268400A1/en
Priority to US13/378,779 priority patent/US20120096600A1/en
Priority to CA2766975A priority patent/CA2766975A1/en
Priority to EP10726917A priority patent/EP2449108A1/en
Application filed by Bayer Bioscience NV filed Critical Bayer Bioscience NV
Priority to IN427DEN2012 priority patent/IN2012DN00427A/en
Priority to CN2010800292508A priority patent/CN102471776A/zh
Publication of WO2011000498A1 publication Critical patent/WO2011000498A1/en
Priority to ZA2011/09371A priority patent/ZA201109371B/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate

Definitions

  • genes containing such introns include the maize sucrose synthase gene (Clancy and Hannah, 2002, Plant Physiol. 130(2):918-29), the maize alcohol dehydrogenase- 1 (Adh-1) and Bronze-1 genes (Callis et al. 1987 Genes Dev. l(10):l 183- 200; Mascarenhas et al. 1990, Plant MoI Biol. 15(6):913-20), the replacement histone H3 gene from alfalfa (Keleman et al. 2002 Transgenic Res.
  • EPSPS is intended to mean any native or mutated 5-enolpyruvylshikimate-3 -phosphate synthase enzyme, the enzymatic activity of which consists in synthesizing 5-0- (l-carboxyvinyl)-3-phosphoshikimate from phosphoenolpyruvate (PEP) and 3-phosphoshikimate (EC 2.5.1.19; Morell et al., 1967, J. Biol. Chem. 242:82-90).
  • said EPSPS enzyme may originate from any type of organism.
  • An EPSPS enzyme suitable for the invention also has the property of being tolerant with respect to herbicides of the phophonomethylglycine family, in particular with respect to glyphosate.
  • the DNA region encoding a 5'UTR may comprise the leader sequence of the chlorophyl a/b binding protein gene from Petunia hyb ⁇ da, more specifically nt 2283-2351 of SEQ ID no. 2.
  • the present invention also relates to plants additionally containing a second chimeric DNA molecule, wherein the second chimeric DNA molecule comprises the following operably linked DNA fragments; a) a promoter sequence of the histone H4 gene of Arabidopsis thaliana; b) a DNA region encoding an intron 1 of a plant replacement histone gene; c) a DNA region encoding a transit peptide;
  • the second chimeric DNA molecule also comprises a DNA region encoding an intron 1 of a plant replacement histone gene, a DNA region encoding a transit peptide, a DNA region encoding a glyphosate-tolerant EPSPS and a 3' transcription termination and polyadenylation region. These DNA regions are similar as described elsewhere in this application.
  • the plant of the invention is a Brassica plant, more preferably an oilseed rape plant.
  • oilseed rape refers to any one of the species Brassica napus, Brassica rapa, Brassica campestris or Brassica juncea.
  • Maize Fungicides Azoxystrobin, Bixafen, Boscalid, Cyproconazole, Dimoxystrobin, Epoxiconazole, Fenitropan, Fluopyram, Fluoxastrobin, Fluxapyroxad, Isopyrazam, Metconazole, Penthiopyrad, Picoxystrobin, Propiconazole, Prothioconazole, Pyraclostrobin, Tebuconazole, Trifloxystrobin
  • Soybean Fungicides Azoxystrobin, Bixafen, Boscalid, Carbendazim, Chlorothalonil, Copper, Cyproconazole, Difenoconazole, Dimoxystrobin, Epoxiconazole, Fluazinam, Fluopyram, Fluoxastrobin, Flutriafol, Fluxapyroxad, Isopyrazam, Iprodione, Isotianil, Mancozeb, Maneb, Metconazole, Metominostrobin, Myclobutanil, Penthiopyrad, Picoxystrobin, Propiconazole, Propineb, Prothioconazole, Pyraclostrobin, Tebuconazole, Tetraconazole, Thiophanate-methyl, Trifloxystrobin t.
  • SEQ ID No.:7 amino acid sequence of the optimized transit peptide TPotp C-IPc
  • T-DNA expression vectors were constructed (pTJN47, pTJN50, pTJN51, pTJN48, pTJN49, pTJN75) comprising the following operably linked DNA fragments:
  • the nucleotide sequence of T-DNA of vector pTJN49 is represented in SEQ ID no. 5.
  • Paragraph 23 A plant according to paragraph 22, wherein the glyphosate-tolerant EPSPS encoding DNA region is adapted to Brassica napus codon usage.

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Cultivation Of Plants (AREA)
PCT/EP2010/003797 2009-07-01 2010-06-24 Methods and means for obtaining plants with enhanced glyphosate tolerance Ceased WO2011000498A1 (en)

Priority Applications (9)

Application Number Priority Date Filing Date Title
EA201270107A EA201270107A1 (ru) 2009-07-01 2010-06-24 Способы и средства для получения растений с повышенной устойчивостью к глифосату
AU2010268400A AU2010268400A1 (en) 2009-07-01 2010-06-24 Methods and means for obtaining plants with enhanced glyphosate tolerance
US13/378,779 US20120096600A1 (en) 2009-07-01 2010-06-24 Methods and means for obtaining plants with enhanced glyphosate tolerance
CA2766975A CA2766975A1 (en) 2009-07-01 2010-06-24 Methods and means for obtaining plants with enhanced glyphosate tolerance
EP10726917A EP2449108A1 (en) 2009-07-01 2010-06-24 Methods and means for obtaining plants with enhanced glyphosate tolerance
JP2012518040A JP2012531216A (ja) 2009-07-01 2010-06-24 グリホサート耐性が増強された植物を得るための方法および手段
IN427DEN2012 IN2012DN00427A (enExample) 2009-07-01 2010-06-24
CN2010800292508A CN102471776A (zh) 2009-07-01 2010-06-24 用于获得具有增强的草甘膦耐受性的植物的方法和手段
ZA2011/09371A ZA201109371B (en) 2009-07-01 2011-12-20 Methods and means for obtaining plants with enhanced glyphosate tolerance

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP09075283 2009-07-01
EP09075283.3 2009-07-01
US27015009P 2009-07-06 2009-07-06
US61/270,150 2009-07-06

Publications (1)

Publication Number Publication Date
WO2011000498A1 true WO2011000498A1 (en) 2011-01-06

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2010/003797 Ceased WO2011000498A1 (en) 2009-07-01 2010-06-24 Methods and means for obtaining plants with enhanced glyphosate tolerance

Country Status (11)

Country Link
US (1) US20120096600A1 (enExample)
EP (1) EP2449108A1 (enExample)
JP (1) JP2012531216A (enExample)
CN (1) CN102471776A (enExample)
AR (1) AR077305A1 (enExample)
AU (1) AU2010268400A1 (enExample)
CA (1) CA2766975A1 (enExample)
EA (1) EA201270107A1 (enExample)
IN (1) IN2012DN00427A (enExample)
WO (1) WO2011000498A1 (enExample)
ZA (1) ZA201109371B (enExample)

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WO2013075817A1 (en) 2011-11-21 2013-05-30 Bayer Intellectual Property Gmbh Fungicide n-[(trisubstitutedsilyl)methyl]-carboxamide derivatives
WO2013079566A2 (en) 2011-11-30 2013-06-06 Bayer Intellectual Property Gmbh Fungicidal n-bicycloalkyl and n-tricycloalkyl (thio)carboxamide derivatives
WO2013098147A1 (en) 2011-12-29 2013-07-04 Bayer Intellectual Property Gmbh Fungicidal 3-[(pyridin-2-ylmethoxyimino)(phenyl)methyl]-2-substituted-1,2,4-oxadiazol-5(2h)-one derivatives
WO2013098146A1 (en) 2011-12-29 2013-07-04 Bayer Intellectual Property Gmbh Fungicidal 3-[(1,3-thiazol-4-ylmethoxyimino)(phenyl)methyl]-2-substituted-1,2,4-oxadiazol-5(2h)-one derivatives
WO2014060518A1 (en) 2012-10-19 2014-04-24 Bayer Cropscience Ag Method of plant growth promotion using carboxamide derivatives
WO2014060520A1 (en) 2012-10-19 2014-04-24 Bayer Cropscience Ag Method for treating plants against fungi resistant to fungicides using carboxamide or thiocarboxamide derivatives
WO2014060519A1 (en) 2012-10-19 2014-04-24 Bayer Cropscience Ag Method for enhancing tolerance to abiotic stress in plants using carboxamide or thiocarboxamide derivatives
WO2014060502A1 (en) 2012-10-19 2014-04-24 Bayer Cropscience Ag Active compound combinations comprising carboxamide derivatives
WO2014170345A2 (en) 2013-04-19 2014-10-23 Bayer Cropscience Ag Method for improved utilization of the production potential of transgenic plants
WO2014177514A1 (en) 2013-04-30 2014-11-06 Bayer Cropscience Ag Nematicidal n-substituted phenethylcarboxamides
WO2014177582A1 (en) 2013-04-30 2014-11-06 Bayer Cropscience Ag N-(2-fluoro-2-phenethyl)carboxamides as nematicides and endoparasiticides
EP2837287A1 (en) 2013-08-15 2015-02-18 Bayer CropScience AG Use of prothioconazole for increasing root growth of Brassicaceae
US9226506B2 (en) 2013-09-25 2016-01-05 Dow Agrosciences Llc Methods of weed control in chicory
WO2019233863A1 (de) 2018-06-04 2019-12-12 Bayer Aktiengesellschaft Herbizid wirksame bizyklische benzoylpyrazole

Families Citing this family (4)

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Publication number Priority date Publication date Assignee Title
CN103074351B (zh) * 2012-10-23 2014-09-24 中国农业大学 人工合成用于转基因抗除草剂植物的epsps基因
AU2015286205A1 (en) * 2014-07-08 2016-12-15 MINISTER FOR AGRICULTURE, FOOD AND FISHERIES acting through THE SOUTH AUSTRALIAN RESEARCH AND DEVELOPMENT INSTITUTE (ABN 53 763 159 658) A BODY CORPORATE PURSUANT TO THE ADMINISTRATIVE ARRANGEMENTS ACT 1994 (SOUTH AUSTRALIA) Herbicide tolerant plants
JP6274161B2 (ja) 2015-06-25 2018-02-07 トヨタ自動車株式会社 薬剤誘導性プロモーター及びこれを用いた遺伝子発現誘導方法
CN109943578A (zh) * 2019-03-21 2019-06-28 浙江大学 一种水稻epsp合成酶突变基因、突变体及其应用

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