WO2010149567A3 - Bioreactor for culturing cells - Google Patents

Bioreactor for culturing cells Download PDF

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Publication number
WO2010149567A3
WO2010149567A3 PCT/EP2010/058552 EP2010058552W WO2010149567A3 WO 2010149567 A3 WO2010149567 A3 WO 2010149567A3 EP 2010058552 W EP2010058552 W EP 2010058552W WO 2010149567 A3 WO2010149567 A3 WO 2010149567A3
Authority
WO
WIPO (PCT)
Prior art keywords
fluid
microchannels
microchambers
bioreactor
culturing cells
Prior art date
Application number
PCT/EP2010/058552
Other languages
French (fr)
Other versions
WO2010149567A2 (en
Inventor
Cécile LEGALLAIS
Régis BAUDOIN
Eric Leclerc
Original Assignee
Universite Technologie De Compiegne - Utc
Centre National De La Recherche Scientifique (Cnrs)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universite Technologie De Compiegne - Utc, Centre National De La Recherche Scientifique (Cnrs) filed Critical Universite Technologie De Compiegne - Utc
Publication of WO2010149567A2 publication Critical patent/WO2010149567A2/en
Publication of WO2010149567A3 publication Critical patent/WO2010149567A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Dispersion Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention relates to a bioreactor for culturing cells, including: a culture chamber comprising a top wall and a bottom wall having a series of microstructures including microwalls (113) and microcontacts (114), the series of microstructures defining microchambers (110) and microchannels (112) on the bottom wall of the culture chamber; a fluid intake connected to at least one portion of the microchannels (112); and a fluid outlet connected to at least one portion of the microchannels (112), the microchannels forming a network connecting the fluid intake to each microchamber (110) and each microchamber (110) to the fluid outlet, enabling the fluid to flow into the microchambers (110), the microchambers (110) each having length and width dimensions relative to a flow direction of the fluid of 500 µm to 550 µm.
PCT/EP2010/058552 2009-06-23 2010-06-17 Bioreactor for culturing cells WO2010149567A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0954288A FR2946984B1 (en) 2009-06-23 2009-06-23 BIOREACTOR FOR CULTIVATING CELLS.
FR0954288 2009-06-23

Publications (2)

Publication Number Publication Date
WO2010149567A2 WO2010149567A2 (en) 2010-12-29
WO2010149567A3 true WO2010149567A3 (en) 2011-12-29

Family

ID=42052771

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2010/058552 WO2010149567A2 (en) 2009-06-23 2010-06-17 Bioreactor for culturing cells

Country Status (2)

Country Link
FR (1) FR2946984B1 (en)
WO (1) WO2010149567A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5881621B2 (en) 2010-03-02 2016-03-09 ユニヴェルシテ テクノロジエ デ コンピエーニュ−ユテセ Multi-reactor box for dynamic cell culture
FR2960062B1 (en) 2010-05-12 2012-12-28 Univ Compiegne Tech TOXICOLOGICAL EVALUATION METHOD, TOXICOLOGICAL SCREENING METHOD, AND SYSTEM THEREOF

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BAUDOIN RÉGIS: "Développement et caractérisation d'une puce à cellules pour le criblage d'agents toxiques", THÈSE PRÉSENTÉE POUR L'OBTENTION DU GRADE DE DOCTEUR DE L'UTC, 24 October 2008 (2008-10-24) - 24 October 2008 (2008-10-24), pages 1 - 183, XP002576413, Retrieved from the Internet <URL:http://tel.archives-ouvertes.fr/docs/00/34/23/42/PDF/Rapport_THESE_Version_finale.pdf> [retrieved on 20100401] *
LECLERC E ET AL: "Human cancerous liver cells culture in a PDMS (polydimethylsiloxane) network of micro channels and chambers", MOLECULAR, CELLULAR AND TISSUE ENGINEERING, 2002. PROCEEDINGS OF THE I EEE-EMBS SPECIAL TOPIC CONFERENCE ON 6-9 JUNE 2002, PISCATAWAY, NJ, USA,IEEE, 6 June 2002 (2002-06-06), pages 104 - 105, XP010625866, ISBN: 978-0-7803-7557-4 *
LECLERC E ET AL: "Perfusion culture of fetal human hepatocytes in PDMS bioreactors", PROCEEDINGS OF THE 25TH. ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY. CANCUN, MEXICO, SEPT. 17 20030917; 20030917 - 20030921 NEW YORK, NY : IEEE, US LNKD- DOI:10.1109/IEMBS.2003.1279468, vol. 2, 17 September 2003 (2003-09-17), pages 1211 - 1214, XP010693033, ISBN: 978-0-7803-7789-9 *

Also Published As

Publication number Publication date
WO2010149567A2 (en) 2010-12-29
FR2946984B1 (en) 2011-08-12
FR2946984A1 (en) 2010-12-24

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