WO2010148540A1

WO2010148540A1 - Fluorescence molecular body imaging system and method thereof

Info

Publication number: WO2010148540A1
Application number: PCT/CN2009/000717
Authority: WO
Inventors: 白净; 汪待发; 刘欣; 刘飞
Original assignee: 清华大学
Priority date: 2009-06-26
Filing date: 2009-06-26
Publication date: 2010-12-29
Also published as: CN102089646A

Abstract

A fluorescence molecular body imaging system and a method are disclosed. The system comprises a line source exciting device (1), a rotating platform (2), a fluorescence imaging device (3), a profile acquiring device (4) and a computer (5). The line source exciting device(1) including a exciting source(11) and a line light beam generating unit(12) is arranged at one side of the rotating platform(2).The rotating platform(2) includes a support(21) used for hanging the imaging object and an electric rotating platform(22) connected to the support(21). The fluorescence imaging device (3) is arranged at the other side of the rotating platform (2) relative to the light source exciting device(1) and includes a lens(31) and a detector(32) used for sampling the fluorescence image data coming from the imaging object. The detector (32) is arranged at rear of the lens (31).The profile acquiring device (4) and the fluorescence imaging device (3) are arranged at the same side to sample 3D profile data of imaging object. The computer (5) is connected electrically with the detector (32) and the electric rotating platform (22) and the profile acquiring device (4) separately by an interface controller. The rotating angles of the rotating platform (2) are preset in the computer (5).

Description

Light splitting system and method

This is a light-splitting system and method for sheep molecular imaging wood, especially for small objects. Existing wood

Molecular imaging wood is the use of or specific cells or cells to obtain bio-physiological and pathological information on living organisms, such as cells, genes, and phase-related information. And new, etc. provide effective information and analyze her means. At present, the image of the image, such as image, image and nuclear magnetic image, which has image, image, and nuclear magnetic image, has been obtained and quickly.

Like in the wood, like the use of

The specific photons incident on the outside are absorbed by the photons of the light. Light and light absorbs part of the photons to the surface of the image and can be detected by a high-performance D-phase ha ge oup ed Dev ce component. F (F uo esce ce o ecu a omog aphy) is the latest light image method with good resolution but near the position where the light source is illuminated. The light in the vicinity of the mega-light source is not easily or the measured image information is not accurate. What's more important is that you can get information about the height of the whole body. It usually takes a week at a certain height. It is necessary to raise the height of the light source of the lifting device of the door to increase the F T image system. In addition, the image of the mantissa N is the same N times. The increase in the existing F T image system and the doubling of the same imaging limit the F T in some of the faster biological sheep phenomenon generations. Content

The purpose of the above is to provide a small image, image, and image of the image and method.

The purpose of the following wood scheme is divided into the image system, which is characterized in that it includes my light source device, special platform, image device, and The device and the light source device are particularly characterized in that they comprise a light source and a beam generating platform, including a bracket for analyzing the image and a special device for connecting the bracket, and the other of the light source devices includes a common integrated image. a detector detector of the photo cluster image is connected to the interface controller of the device and the integrated interface of the device, respectively, and the input image device of the special angle is connected to the detector, the device and the device respectively Imaging of the image of the light and the imaging of the device

The output control is specific to, and the work instructions of the device and device and the distribution of the photon image reconstruction within the image.

The high-power LED beam in the light source or the optical band is formed into a circular one end and the other end of the square glass 仟 light beam is formed by a circular outer slit.

Light source I have a beam generator.

In particular, there is a filter.

Detector one phase.

The device is connected to the image of the analysis.

The system of light molecular suspension method includes the following steps including: my light source device, special platform, image detecting device, device and optical molecular imaging system interface controller respectively connected to the special platform, the image device and the device 2 using the image 2 Like the blame on the special platform, the 3 light is divided into the special system of the rest system, and the special image is used to stop the image and contour of the special imaging.

Special special 36.

0 Stop 4 The photon image to be used is distributed in the image using the rest reconstruction method.

In step 4, the method includes the following steps: forming a surface of the image to be imaged by the device, and then illuminating the model with a finite element, the position of the light source of the light source device, and the position of the fixed light source of the light source. The image of the medium to the image of the surface of the image is solved on the finite element and the source The Green's function distribution Q is obtained by using the obtained Green's function cut distribution to obtain the same intensity of her intensity distribution. According to her intensity distribution, I get the distribution within the image.

Because of the above wood scheme, it has the following, because it is due to the whole body of the light source, so you only need to use the small 360.

The convenience of the increase in the throughput of the city has increased the speed of the image. 2 Because there are special special angles in the present invention

The number of devices and devices is also controlled so that the measurement image is high. 3. This method improves the image in one step because of the reconstruction of the device and the reconstruction in the small work. 4 Since the light source device, the image device, and the small utility platform device are in a non-connected, full-body, 36.

The system of 0 panoramic measurement has greatly improved the convenience of the light source detector and the same number. 1 is the present group

Group 2 is a schematic diagram of the light source device in the present invention.

3 is , and the light characteristics

4 is the signing of Ben Hugh

Group 5 is 4

6 is the indication of Ben Hugh

7 is 6 overlooking

8 is the distribution of the measured height a

9 is the distribution of the measured height b

0 is the measured height of Hugh. The distribution image 1 is the distribution of the measured height a

2 is the measured height of b. The distribution of image 3 is the measured height. Distribution like this best way The following group and the original.

As shown, the system includes a xenon light source device 1, a small utility platform 2, an image device 3, and devices 4 and 5.

The light source device 1 includes a light source 11 and a beam generation 12 on the small utility platform 2 and a filter 13 at the same time as the light source 1 and the beam generation 12 so that only the pupil beam within the small power cycle generates 12 and filters out the others. As shown in 2, the light source 11 can

1 It is also possible to use high-power LEDs in the optical band. The beam generation 12 can be one end round and the other end square glass 12

The light emitted by 11 has a small light beam slit 122 to obtain the light source required by the system of the present invention. The gap 122 4 m is actually mm. My light source unit 1 can also be used as a device for other light sources, as long as the generated light beam is less than mm.

As shown, the small work 2 includes a bracket 2 for a small utility and a special 22 for connecting the bracket 2. The small utility pass bracket 21 in the special 22 her position in the strange hundred pass position of the small center of the rest of the axis of the special 22.

The other device 3 of the small-power platform 2 of the phase light source device includes a large value of 3 and a detector 32 located at 31 with a sum of 33 and . This is a 32-phase.

The device 4 is synchronized with the photoreceiving device 4 of the detector 32. The device 4 is connected to the image of 5 .

5 The interface controller is connected to the image of the small work surface of the detector 32 and the device 4 and the small work of the device 4 to the reconstruction of the small work of the device 32 to be reconstructed in the form of an image. In addition, there is a special angle of 5 in the 5 interface controller to connect the special 22 in the small platform 2 to control the special 22 small work, the body is in accordance with the special angle within 5, especially the small work panorama or 360 degree light group Like and. The system uses the light filter 13 from the light source 1 to filter out My beam-forming sheep 2 special light source illuminates the filter on the surface of the break in the break. The filter is filtered and the light is received by the detector 32. The same device 4 will also deliver the synchronous small things. Take a look at the 5 special 22 special angle to stop the special 5 control detector 32 and the number of the phase of the device 4 until the special 22 total 360 stop.

5 The back projection method is used to get the surface of the small object. The best way to rebuild is to get the distribution in Xiaogong.

This method includes the following steps

Xiaogong puts a specific injection into the small work.

2 Fixing the small work The ramification of the small things with the bracket 21 is strange. On the special 2, the central axis of the small power is the same as that of the special 22.

3 小功 Under the control of 5, see the special 22 light source device 1 to pay for the special surface of the small rest

The special image device 3 stops the image of the aperture on the surface of the small image. The 5 device 4 is synchronized.

Will 5, special 22 special 360 complete the light group image.

4 The photon image that is used depends on the distribution of the rest of the work in the small work break.

The surface of the small work to the device 4 is made into a model G, and then the model G is a finite element.

(The detector 32 is connected to the light source of the light source device. The position of the light source of the fixed length of the light source is irradiated onto the surface of the beam of the small work contour. In the middle of the light beam, the "any" function in the middle e Satisfied as follows

L " 1

K2e e

" ge condition, the light source is evenly distributed and the energy and force are not in the same place. Above the energy force 0.

The image of the detector 32 is solved on the finite element F. My light source "and its Green's function distribution Green's function cut distribution satisfies the following differential equation

Where ") is located at the position where her coefficient is located at the position of her absorption coefficient and the uniformity is known. G Green's function tangent distribution S. Solving the Green's function of the 光源 source Distribution G S

"L" solves a certain position, her

The Green's function distribution S. Where P is a function which satisfies the following

Which represents the same. On the condition P is in her energy level in other energies 0.

Each light source uses the obtained Green's function to cut the distribution to obtain her intensity. The unknown distribution between the two is the rest of the six-faced Hugh AV. . . T. Shang Xiu is like Xiao Xiaoxiu's model G, who is building her strength on Six Faces. Six-faced Hugh Center

The matrix W of the same distribution of juice on the upper position is as follows:

Where B, J 12. . . T is in the center of the six-faced Hughes, her W e, my light source "in the center of the six-faced Hughes, her table of strength to her is as follows

W e J Gw G ,

All the money sources "and all of them can be based on the matrix. The following is the basis of my equation. The vector b represents all the laughed numbers. The matrix force is one row. The matrix of the light source.

Solving the domain equation b A gives the distribution of the probe on the six-sided Hugh Center. The effectiveness of the system and method of the present invention is as follows. Use docya e ee as shown

3 US SEM FF 775 46 33 with US e e ock company FF 840 12 detector 2 solid ndo company 897 phase 31 using N kko 6 2. D of Japan N ko company. The light characteristic black 哉 as shown in 3 indicates the light latent characteristic of 13

33 light characteristics I said.

As shown in Figure 4, in the rest, the "column coefficient of the position "1 3m absorption coefficient 0.0 2m" filled with the fat solution of the fat solution is 6 mm. The glass cylinder 6 is straight to the height of 68 m 68 m. It will be filled with u, g mL, glass tube 7 probes inside 3m. The glass tube 7 is placed in the glass cylinder 6 as if it were injected in a small work break. The light beam is 4 m mm and the height of the bottom of the glass cylinder 6 is 3 . Special 22 within 5.

At a special angle of 10, the detector 32 has a total of 36 images. It will be like a small Hugh A =.5 mX .5mmX .5 m six-sided Hugh. The light source from the xenon light source device 1 is placed every 2m in the 60 gardens around the center of the glass cylinder 6.

As shown in Figures 4 and 5, she is placed in the bottom 38 of the glass cylinder 6 at her position. As shown in Figure 67, she is placed in the bottom 24 of the glass cylinder 6 at her position. The upper case of God uses the method of 50 T geb a c eco s uc on ech que algebraic reconstruction to obtain a three-dimensional distribution within the glass cylinder. The reconstruction obtained in the glass cylinder 6 is at a height of a 38 m 3 m) C 24 )

Like 8 10, 1 3 respectively, the system and method of this invention are effective.

The various parts, positions, and connections of the above components may be modified. The modifications and equivalent exchanges of the components of the present invention are not excluded from this warranty.

Claims

Requirements 1. The optical molecular imaging system is characterized in that it includes a light source device, a special platform, an image device, a device, and

a light source device in which it includes a light source and a beam generating sheep

The special platform includes a support bracket and a special bracket.

Another detector that is mounted on the special platform of the phase light source device, including the image of the image of the image of the confocal image.

The interface controllers that image the same image of the device are connected to the detectors, the special angles of the input device, and the imaged image of the image to which the device is connected.

The output of the imaged device is controlled to control the distribution of the work orders of the particular platform, image device and device, and the image within the image.

2. The required optical molecular imaging system is characterized by

The high-power LE 哉 beam in the light source or in the optical band produces a circular glass bulb at the other end of the square.

3. An optical molecular imaging system of claim 1 is characterized in that

The light source is generated with a filter.

4. A light division of claim 2 is applied to the image system.

The source beam is generated with a filter.

5. A light splitting requirement of 1 or 2 or 3 or 4 is unique to the rest image system.

6. A photomolecular imaging system requiring 2 or 3 or 4 is characterized by a phase of the detector.

7. An optical molecular imaging system of claim 5 is characterized by a detector-phase D.

8. A light splitting of 2 or 3 or 4 or 7 is required for the image of the image system.

9. A light division of claim 5 is applied to the image system.

The image of the device.

10. An optical molecular imaging system of claim 6 is characterized in that

The image connected to the device.

The system of light is divided into the method of rest, which includes the following steps.

1 The optical molecular imaging system interface controllers including my light source devices, special devices, devices, devices, and devices are connected to special platforms, image devices, and devices.

2 use image

2 will be like a straight platform

3 Optical Molecular Resting System Control The special platform of the special special platform stops the aperture image of special imaging and

Special platform Special 360.

stop

4 The photon image that is used depends on the reconstruction method. The probe is distributed in the image.

12 A photomolecular image capture method of claim 1 is characterized in that step 4 is performed, which comprises the following steps

The surface of the image to which the device is placed is put into a feast model, and then the model is transformed into a finite element.

The image is illuminated by the light source, and the position of the fixed light source is irradiated into the beam of the image.

The image to which the detector arrives solves the distribution of the light source and its Green's function on the finite element

Each 哉 light source is obtained by using the obtained Green's function cut distribution

Got Distribution within the image.