WO2010140774A3 - Protein analysis method in which a data-independent analysis method and a data-dependent analysis method are combined - Google Patents

Protein analysis method in which a data-independent analysis method and a data-dependent analysis method are combined Download PDF

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Publication number
WO2010140774A3
WO2010140774A3 PCT/KR2010/002745 KR2010002745W WO2010140774A3 WO 2010140774 A3 WO2010140774 A3 WO 2010140774A3 KR 2010002745 W KR2010002745 W KR 2010002745W WO 2010140774 A3 WO2010140774 A3 WO 2010140774A3
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WO
WIPO (PCT)
Prior art keywords
analysis method
data
protein
peptide
qualitative
Prior art date
Application number
PCT/KR2010/002745
Other languages
French (fr)
Korean (ko)
Other versions
WO2010140774A2 (en
Inventor
권요셉
박치열
노국필
이태훈
최종순
Original Assignee
한국기초과학지원연구원
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 한국기초과학지원연구원 filed Critical 한국기초과학지원연구원
Publication of WO2010140774A2 publication Critical patent/WO2010140774A2/en
Publication of WO2010140774A3 publication Critical patent/WO2010140774A3/en
Priority to US13/309,038 priority Critical patent/US20120109533A1/en

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Classifications

    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/0027—Methods for using particle spectrometers
    • H01J49/0036—Step by step routines describing the handling of the data generated during a measurement
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/62—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode
    • G01N27/622—Ion mobility spectrometry
    • G01N27/623—Ion mobility spectrometry combined with mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/0027—Methods for using particle spectrometers
    • H01J49/0031—Step by step routines describing the use of the apparatus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins

Abstract

The present invention relates to a method for analyzing proteins using a mass spectrometer, and to a qualitative and quantitative protein analysis method in which a data-independent analysis method and a data-dependent analysis method are combined, wherein said qualitative and quantitative protein analysis method comprises: (A) a step of pretreating a protein mixture; (B) a step of acquiring information on a peptide retention time and on a mass value of the peptide through data-independent analysis; (C) a step of searching a database (PLGS) on the basis of the information on the peptide retention time and on the mass value of the peptide, and performing a first qualitative and quantitative analysis on the protein; (D) a step of extracting information on a predetermined protein from the information on a peptide retention time and on a mass value of the peptide; (E) a step of conducting a data-dependent analysis using the information extracted in step (D); (F) a step of searching a database (MASCOT) on the basis of the information on a peptide retention time and on a mass value of the peptide, acquired in step (E), and performing a second qualitative and quantitative analysis on the protein; and (G) a step of comparing the result of step (C) and the result of step (F) to verify the qualitative and quantitative analysis on the protein.
PCT/KR2010/002745 2009-06-01 2010-04-30 Protein analysis method in which a data-independent analysis method and a data-dependent analysis method are combined WO2010140774A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US13/309,038 US20120109533A1 (en) 2009-06-01 2011-12-01 Method of analyzing protein using data-independent analysis combined with data-dependent analysis

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020090048024A KR101114228B1 (en) 2009-06-01 2009-06-01 Protein identification and their validation method based on the data independent analysis
KR10-2009-0048024 2009-06-01

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US13/309,038 Continuation US20120109533A1 (en) 2009-06-01 2011-12-01 Method of analyzing protein using data-independent analysis combined with data-dependent analysis

Publications (2)

Publication Number Publication Date
WO2010140774A2 WO2010140774A2 (en) 2010-12-09
WO2010140774A3 true WO2010140774A3 (en) 2011-03-10

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2010/002745 WO2010140774A2 (en) 2009-06-01 2010-04-30 Protein analysis method in which a data-independent analysis method and a data-dependent analysis method are combined

Country Status (3)

Country Link
US (1) US20120109533A1 (en)
KR (1) KR101114228B1 (en)
WO (1) WO2010140774A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101431062B1 (en) 2012-03-08 2014-08-21 (주)바이오메디앙 Multiple biomarker set for breast cancer diagnosis, method of detecting the same, and diagnosis kit for breast cancer using antibody against the same
US10495647B2 (en) 2014-06-13 2019-12-03 Waters Technologies Corporation Analysis of complex biological matrices through targeting and advanced precursor and product ion alignment
JP6698668B2 (en) * 2015-02-05 2020-05-27 ディーエイチ テクノロジーズ デベロップメント プライベート リミテッド High-speed scanning of wide quadrupole RF window while switching fragmentation energy
US9911585B1 (en) * 2016-12-21 2018-03-06 Thermo Finnigan Llc Data-independent mass spectral data acquisition including data-dependent precursor-ion surveys
JP7400698B2 (en) * 2020-11-16 2023-12-19 株式会社島津製作所 chromatography mass spectrometer

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030013120A1 (en) * 2001-07-12 2003-01-16 Patz Edward F. System and method for differential protein expression and a diagnostic biomarker discovery system and method using same
KR20030074773A (en) * 2001-02-01 2003-09-19 싸이퍼젠 바이오시스템즈, 인코포레이티드 Improved methods for protein identification, characterization and sequencing by tandem mass spectrometry
KR20070029126A (en) * 2003-12-17 2007-03-13 요시오 야마우치 Protein analysis method
JP2007256126A (en) * 2006-03-24 2007-10-04 Hitachi High-Technologies Corp Mass spectrometry system

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5538897A (en) * 1994-03-14 1996-07-23 University Of Washington Use of mass spectrometry fragmentation patterns of peptides to identify amino acid sequences in databases

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20030074773A (en) * 2001-02-01 2003-09-19 싸이퍼젠 바이오시스템즈, 인코포레이티드 Improved methods for protein identification, characterization and sequencing by tandem mass spectrometry
US20030013120A1 (en) * 2001-07-12 2003-01-16 Patz Edward F. System and method for differential protein expression and a diagnostic biomarker discovery system and method using same
KR20070029126A (en) * 2003-12-17 2007-03-13 요시오 야마우치 Protein analysis method
JP2007256126A (en) * 2006-03-24 2007-10-04 Hitachi High-Technologies Corp Mass spectrometry system

Also Published As

Publication number Publication date
KR20100129457A (en) 2010-12-09
WO2010140774A2 (en) 2010-12-09
US20120109533A1 (en) 2012-05-03
KR101114228B1 (en) 2012-03-05

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