WO2010134104A1 - Système d'excitation à fluorescence multibande modulaire - Google Patents

Système d'excitation à fluorescence multibande modulaire Download PDF

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Publication number
WO2010134104A1
WO2010134104A1 PCT/IT2009/000225 IT2009000225W WO2010134104A1 WO 2010134104 A1 WO2010134104 A1 WO 2010134104A1 IT 2009000225 W IT2009000225 W IT 2009000225W WO 2010134104 A1 WO2010134104 A1 WO 2010134104A1
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WO
WIPO (PCT)
Prior art keywords
light
leds
light source
excitation
led
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PCT/IT2009/000225
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English (en)
Inventor
Gian Luca Ferri
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Universita' Degli Studi Di Cagliari
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Priority to PCT/IT2009/000225 priority Critical patent/WO2010134104A1/fr
Publication of WO2010134104A1 publication Critical patent/WO2010134104A1/fr

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Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy

Definitions

  • This invention concerns a modular multi-band fluorescence excitation system. More specifically, the invention concerns a multi-band fluorescence excitation system suitable to collect together light from multiple LEDs (Light Emitting Diodes), hence combining light bands of the same, and/or partly superimposed, and/or different well determined wavelength intervals, to provide a mixed light beam of high intensity, and comprising the different wavelength intervals as required, such system being modular so as to be flexibly used.
  • LEDs Light Emitting Diodes
  • Fluorescence based methods are used in the bio-medical field to observe or reveal a variety of natural, genetically introduced, or exogenous fluorescent molecules (or fluorochromes), the latter two being used to label cell and tissue components directly or by means of specific antibodies or other molecular probes.
  • Fluorescence microscopes and related equipment are used to reveal fluorochromes within cell and tissue preparations, from very low (life size) to high magnification, and to record and/or analyse the relevant images.
  • multiple-analyte methods e.g. gene arrays, protein- and antibody- arrays
  • the possibility of labelling and observing or revealing at the same time multiple (i.e. more than 2 or 3) different fluorescent molecules or labels in one and the same tissue or cell preparation is under increasing demand, since it provides a unique amount of information as to the simultaneous presence and spatial relationships between multiple cellular components and molecules of interest.
  • fluorescence visualization and/or imaging methods are that: (i) an appropriate selection of the fluorescence excitation wavelength intervals used results in the selective excitation of the wanted one/s within a mixture of different fluorochromes, and: (ii) an appropriate selection of the emission wavelength ranges transmitted to the eye or imaging system results in the visualization or imaging of the wanted one/s within said mixture of different fluorochromes.
  • all fluorochromes show extended spectral regions of low-level excitation and/or emission (excitation and emission tails), so that their effective separation requires carefully chosen wavelength ranges on both the excitation as well as the emission side.
  • Said excitation light intervals will need to be: (i) highly discreet and separate from each other, i.e. showing high intensity in the wanted excitation interval, said intensity very rapidly decreasing at the extremes of the wanted interval (e.g. to 10exp(-6) or lower, within a few nm from the wanted band limit); (ii) available in multiple different intervals, including closely adjacent or partly overlapping ranges as may be required for specific fluorochromes; (iii) each rapidly switchable on-and-off, so that the selective visualization of single, as well as specific groups of fluorochromes can be accomplished at ease and repeatedly during observation or imaging sessions; (iv) each finely tunable in intensity, from maximum to very low intensity, in a way entirely independent from all other excitation bands.
  • a reflected (or incident) fluorescence visualization or imaging system such as a fluorescence microscope, is essentially composed of: (a) a light source; (b) optics to convey light into an excitation light pathway, generally as parallel (collimated) light upon reaching the excitation filter; (c) an excitation filter to select one or more excitation wavelength bands to be conveyed to the object; (d) a dichroic mirror, i.e.
  • a device which will reflect the excitation wavelength band/s to the object, while transmitting the fluorescence emission wavelength/s from the object to the emission optical pathway; (e) optics to focus excitation light onto the object and to collect fluorescence emission light from said object; (T) an emission, or barrier, filter to block any stray excitation light wavelength/s transmitted into the emission optical pathway, while letting through the fluorescence emission light; (g) optics to convey the fluorescence emission wavelength/s to the eye and/or to a camera or other recording apparatus.
  • the excitation light can be projected directly onto the specimen, while the emission light follows the same pathway as above, hence is collected by optics, and is transmitted through an emission filter to the visualization or imaging optics.
  • Multiple-band excitation filters are especially critical, since they must change from high transmission (>80-90%) to very high rejection ( ⁇ 10exp(-6) transmission or so) within a few nm, at each and every interface between a transmission region (which lets through light in a wanted excitation interval) and the adjacent high rejection regions.
  • Such high-rejection spectral regions in the excitation filter correspond to high- transmission regions in the dichroic mirror and emission filter, for collection and visualization of the far weaker light emitted by the fluorochrome/s being studied.
  • LEDs Light Emitting Diodes
  • LEDs are in principle especially suited for such purpose, in view of their intense emission in comparatively narrow wavelength ranges (e.g. from 5-10, to
  • LEDs are easily controlled and finely tuned by electronic driving circuitry, and can be switched on and emit light very rapidly ( ⁇ 100 nsec). Also, LEDs can be flash operated at high current, high light output for short to very short time periods, hence a set-up comprising parallel LEDs would permit exposure of the specimen to multiple coincident or synchronized pulses of high-intensity light of controlled wavelength/s.
  • LED based light sources have been proposed as follows: (i) multiple, different wavelength/colour LEDs arranged in groups or arrays, to permit regulation of colour balance in the overall light output (WO2007/111735A2, WO2006/136406A1 , CN2630877YY);
  • a LED with its individual optics, or with its individual optics and its band selection filter (excitation filter), or multiple, interchangeable such combination of LED, optics and filter is/are used as a single-band source, or as multiple, interchangeable single-band sources for fluorescence microscopy, using either transmitted light excitation (WO2006/072886; US2007/0211460), or incident/reflected light excitation (WO2004/088387, US2007/0053058), or is sent to the specimen via an independent optical pathway (JP2006301523A, US2008/0043324A1, US2008/0013166A1 , WO2007/090591A1 , EP1528421 B1 , US20G4/0061070A1);
  • LED excitation modules can be effectively combined by means of dichroic mirrors, so that the latter approach can address the simultaneous visualization or imaging of an equivalent number of fluorochromes.
  • individual LEDs emitting at different wavelengths, their optics, and the relevant wavelength specific excitation filter, dichroic mirror, as well as emission filter are assembled together in further commercially available set-ups, to compose interchangeable sets for use in a fluorescence visualization/imaging system, such as a fluorescence microscope, much the same way as "fluorescence filter cubes" are commonly used and interchanged.
  • LED driving electronic circuitry is arranged in such a way as to be able to provide high current, short to very short duration power pulses to any number of wanted, simultaneously, synchronously or sequentially operated LEDs. It is also part of the present invention that said driving electronic circuitry is interfaced to a computer, for system operation, control and/or programming.
  • each and every of said excitation wavelength 5 ranges is rapidly switchable on-and-off and tunable in intensity, in such a way as not to interfere with any of the other simultaneously operated excitation bands.
  • each light source module comprising a LED mounting support, onto which an array of LEDs are mounted, and being mounted in turn onto a module mounting support, each one of said LEDs being capable of emitting a light beam at a wavelength range, and in that said filtering means comprise one or more modular excitation band selecting filter
  • each excitation band selecting filter element having a light receiving area and being capable to receive the light beam of each one of said LEDs of the respective light source module on a corresponding portion of said light receiving area, whereby selecting a band of the light beam wavelength
  • said LEDs and said excitation band selecting filter elements could be arranged in such a way that the light beam emitted by each one of said LEDs has an angle of light incidence of substantially 90°, normal incidence, with respect to the30 corresponding portion of said light receiving area of said excitation band selecting filter element, said angle of light incidence being comprised within such a maximum deviation from said normal incidence such that the spectral selection operated by said excitation band selection filter elements is substantially superimposable for all light beams from any LED in each light source module.
  • said light source modules could be removably arranged side by side, so as to form a matrix composed of interchangeable light source modules
  • said excitation band selecting filter elements each one corresponding to one light source module, are removably arranged side by side, so as to form a matrix composed of interchangeable excitation band selecting filter elements.
  • said system could comprise one or more light beam separators for preventing the diffusion of stray light among LED light beams.
  • each one of said excitation band selecting filter element could be arranged at a respective first angle of tilt with respect to said overall axis of the system, so that the angle of incidence of each light beam from each one of said LEDs is of substantially 90° with respect to the corresponding portion of said light receiving area of the relevant excitation band selecting filter element.
  • each one of said LEDs could comprise its own collimating and projecting optics, each one of said LEDs being arranged at a respective, individual second angle of tilt with respect to the said LED mounting support in said light source module, so that each
  • LED's light beam is aligned with the LED's collimating and projecting optics, in such a way that the light beam from each one of said LEDs is collimated by said optics of each LED and projected to the corresponding portion of said light receiving area of said excitation band selecting filter element, therethrough converging onto the light mixing means.
  • said module mounting support could be so shaped that each light source module is interchangeably mounted at a respective third angle of tilt with respect to said overall reference axis, so that the light beams from all said LEDs in each light source module are collimated, projecting onto the corresponding portion of said light receiving area of said excitation band selecting filter element, therethrough converging together onto said light mixing means.
  • the LEDs of each light source module emitting in substantially the same wavelength range could be mounted at adjacent locations, and the corresponding excitation band selecting filter element area portions are adjacent as well and/or compose a single homogeneous area of said filter.
  • said system could comprise first optics for collimating said output light beam coming from said mixing means.
  • said system could comprise second optics arranged between said filtering means and said mixing means, whereby reducing the overall length and size of said system, without increasing the maximum deviation from a 90° angle of light incidence, normal incidence, of said light beams at the entry of said mixing means.
  • said mixing means could comprise an optical rod and/or an optical fibre and/or a bundle of randomly arranged optic fibres or of optical rods, so that said at least one mixed output light beam is obtained by multiple internal reflections.
  • said system could comprise electronic driving circuitry connected to each one of said LEDs of said light source modules, said electronic driving circuitry being capable to switch on and off each LED and to regulate the light output intensity and the switching frequency, so as to switch on-and-off and tune the intensity of the light of each and any excitation band of each LED without interferences with any of the other excitation bands obtained from other LEDs, said driving circuitry is capable to be interfaced to a computer, for system operation, control and/or programming.
  • said LED mounting supports are heat-transmitting
  • said module mounting support is heat-transmitting
  • the system comprises fins coupled to said module mounting support and/or forced ventilation means and/or cooling fluid means and/or heat pipes and/or Peltier.
  • said system could be coupled to an observation or visualization device, or to a microscope, which includes relevant optics and/or multi-band dichroic mirror/s to reflect the excitation light beams onto a specimen, and/or multi-band emission filters to permit observation or imaging of the relevant excited fluorochrome labels.
  • figure 1a shows a schematic view of a first embodiment of a modular multi-band fluorescence excitation system according to the present invention
  • figure 1b shows a schematic view of the system according to figure
  • FIG 1a it is shown a modular multi-band fluorescence excitation system, which has an overall reference axis A and comprising a light source LED-module V, which in its turn comprises, by way of example, eight LEDs 3', each one coupled to a first heat-transmitting support 2'. Moreover, each LED 3' comprises its own light collecting, collimating and projecting optic 4'.
  • a light source LED-module V which in its turn comprises, by way of example, eight LEDs 3', each one coupled to a first heat-transmitting support 2'.
  • each LED 3' comprises its own light collecting, collimating and projecting optic 4'.
  • Light beams from said LEDs 3' are hence projected to an excitation band selection interference filter element 6'.
  • the arrangement and the dimensions of the set-up are such that light from even the most lateral
  • LEDs 3' in the light source module V (in the figure the uppermost and lowermost LED 3', respectively) will reach the filter receiving area at an angle of about 90°, precisely at an angle different from 90° (normal incidence) within such a maximum extent as to ensure that the spectral selection operated by the excitation band selection interference filter will be superimposable for all light beams from any LED 3' of the light source module 1'.
  • a light beam separator 5 is provided to prevent diffusion of stray light between the single-LED 3' light beams and other LEDs' filter 6' area portions.
  • Single-LED 3' light beams are hence collected together to form a mixed light beam, and an even distribution of light from each LED 3' within the mixed beam is obtained by multiple internal reflections within said optical rod or optical fibre 7. Bending or arching of said optical fibre 7 onto at least two perpendicular planes may be aimed at increasing the effect of internal reflections and light mixing within said mixed light beam.
  • mixed light is collimated by optics 8 and is sent to a specimen (not shown in the figure), or to the excitation path of a fluorescence visualization or imaging system, such as a reflected fluorescence microscope.
  • Figure 1b shows a plurality of light source modules 1 '...1 ⁇ arranged side-by-side, so as to form a matrix light source.
  • Said light source modules ⁇ 1'...1 n are coupled to a second heat-transmitting and/or heat-dissipating support 9.
  • the excitation light selecting filters 6'...6 n (each one of said selecting filters 6'...6 ⁇ corresponds to one light source module 1'...1 n ) are mounted side-by side immediately after the light separator 5, which prevents stray light diffusion between individual LEDs 3, as well as between said light source modules 1 '...1 n .
  • the arrangement and the dimensions of the set-up are such that light from even the most lateral LEDs 3 in the most lateral light source modules 1 will reach the respective filter element 6 surface at an angle of substantially 90°, and within such an allowable maximum deviation from 90°, that the spectral selection operated by said filter 6'...6 n will be superimposable for all light beams derived from any LED 3 in the different light source modules 1.
  • Said second heat-transmitting support 9 supports the light source modules 1 and dissipates heat into air by fins, with or without a forced ventilation.
  • heat will be dissipated by means of a circulating coolant, or heat-pipe/s and an appropriate heat-exchanger, with or without an intervening element to allow heat exchange and to maintain LEDs 3 at a low temperature, such as a Peltier or similar elements.
  • the set-up is intended to accommodate and collect together light from LEDs 3 emitting at different, as well as at more or less extensively superimposed, or at identical wavelengths.
  • one such light source module 1' could comprise four identical (i.e.
  • the relevant excitation band selection interference filter (6', the figure shows its light-receiving surface) comprises several distinct areas of differing surface treatment (hence of differing spectral behaviour), which in the above example are intended to select the same excitation band from the group of four identical LEDs 3a, i.e. "a" area portion of the filter 6' is arranged to receive the light from the four identical LEDs 3'a, while "b" and “c” area portions of the filter 6' are arranged to select the light from the other two groups of identical LEDs 6'b and 6'c respectively.
  • this filter comprises one light selection area 6'd identical throughout the filter, being intended to work with multiple identical LEDs 3' to obtain a higher light output intensity.
  • Each of said filter elements 6'...6 n , and/or each homogeneous excitation light selecting portion area of each said filter element 6'...6 ⁇ will be designed and produced according to state-of-the- art technology to yield both high transmission as well as high spectral resolution at both edges of the desired transmission band.
  • filter elements 6'...6 n each serving its intended light source module 1 '...1 n may be made to mount individual, removable and interchangeable single LED f ⁇ lter/s or filter areas (not shown).
  • Light collected and collimated from LED 3'...3 n sources will contain at least partly non parallel light beams, while light beams deriving from centrally located versus laterally located LEDs 3'...3 n and LED light source modules 1'...1", will reach the corresponding portion of the light receiving area of the filter element 6'...6 n at differing angles of incidence. While the overall system will be aimed at minimizing such variations in light incidence angles, filter elements will be designed according to available surface coating technology to minimize the spectral effects of such varied light incidence angle.
  • Electronic driving circuitry is also part of the preferred embodiment, to switch each LED on and off, as well as to regulate its light output intensity by high frequency switching.
  • LED driving circuitry is to be arranged in such a way as to be able to provide high current, short to very short duration power pulses to any number of wanted, simultaneously, synchronously or sequentially operated LEDs.
  • Said driving electronic circuitry is also intended to be interfaced to a computer, for system operation, control and/or programming.
  • each and every of said excitation wavelength ranges is rapidly switchable on-and-off, and tunable in intensity, without interfering with any of the other excitation bands being operated, or potentially available, nor requiring per se the interchange of optical or other parts in the visualization or imaging system or microscope.
  • each and all excitation band/s can be rapidly and independently adjusted in intensity, or switched on-and-off entirely as required.
  • the resulting mixed excitation light beam can include any desired combination of wavelength ranges, including multiple adjacent, and/or partly or completely superimposed wavelength ranges, for simultaneous and/or sequential excitation of one, two, or multiple fluorescent molecules or probes, Said mixed excitation light beam is then sent to the object of interest by means of relevant optics, or reflected by appropriate multi-band dichroic/s, included in a further observation or visualization system, or in a microscope's excitation pathway, hence focussed onto the specimen, to excite the relevant fluorescent molecules.
  • FIG 2a e 2b it is shown a second embodiment of the invention.
  • the system is provided with additional optics 10 to reduce the overall length and size, without increasing the maximum angle of light incidence at the entry of the optical rod or fibre 7, hence maintaining such maximum angle of incidence within the required limits for an efficient entry into the optical rod or fibre 7, of light from each and all LEDs 3 and light source modules 1 '...1 ⁇ .
  • light beams from the different LEDs 3 of light source modules 1'...1 n is converged by said additional optical element/s 10, hence collimated to the entry of said optical rod or fibre 7.
  • This embodiment may also allow the use of a large number of LEDs 3 and/or light source modules 1'...1 n , so that the angle of light incidence for light beams from the most lateral LEDs 3 and light source modules 1'...1 n remains within an allowable range, without increasing the overall length and/or size of the system.
  • FIGS 3a and 3b show a third embodiment of the invention, suitable to maintain the angle of incidence of the LEDs' light beams onto the selecting filter elements 6'...6 n closer to 90° (normal angle), as well as to make each LED's optics operate in axis with its LED light beam, or close to it.
  • each single LED 3, each light source module 1 "...1 ⁇ , as well as each module's filter element 6'...6 n , or any of these are mounted at their own proper angle of tilt each, with respect to said overall reference axis A of the system, said angle depending on the distance of said LED 3, light source module 1 '...1 n , and/or filter element 6'...6" from the overall optical axis of the system.
  • the angle of incidence of light from the most lateral LEDs 3 and light source modules 1'...1 n onto their respective portions of the light receiving area of the excitation light selecting filter will be made to be substantially close to 90° (normal incidence); and - single-LED optics will be made to work in axis to the LED 3 emission axis, or close to it.
  • each light source module 11 is, by way of example, so shaped, as to provide appropriately oriented mounting and heat transfer surfaces for each individual LED 3 in the light source module 1'.
  • the heat-transmitting and/or heat-dissipating support 9 onto which the various LED modules 1'...1 ⁇ are mounted is also shaped in such a way, as to provide appropriately oriented mounting and heat transfer surfaces for each individual light source module 1'...1 n .
  • each filter element 6'...6 n is mounted at an appropriate angle of tilt, always with respect to said overall reference axis A, adjacent to the light beam separator 5, or onto it.
  • the present system has many advantages, among which the following ones are recalled:
  • each and every one of said excitation bands independently "on-off” switchable, as well as finely tunable in intensity;
  • - by means of said excitation band and mixed light beam it is capable of exciting and revealing a multiplicity of fluorescent molecules of appropriately differing spectra, either singly or simultaneously (i.e. at one and the same time);
  • each light source module can be interchanged with others, or novel ones can be added or mounted, in a simple and quick way, hence providing an easily tailored system suitable to satisfy a wide diversity of requirements, as well as to permit easy and economical updating to new or novel requirements, e.g. new excitation wavelength intervals for novel fluorochromes.

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Optics & Photonics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Microscoopes, Condenser (AREA)

Abstract

L'invention porte sur un système d'excitation à fluorescence multibande, ledit système ayant un axe de référence global (A) et comprenant une source de lumière (1 '...1n, 3'...3n) délivrant des faisceaux de lumière, des moyens (6'...6n) pour filtrer lesdits faisceaux de lumière de ladite source de lumière, et des moyens (7) pour mélanger lesdits faisceaux de lumière provenant desdits moyens de filtrage (6'...6n), afin d'obtenir au moins un faisceau de lumière de sortie ayant une combinaison de plages de longueurs d'onde. Le système selon l'invention est caractérisé en ce que ladite source de lumière comprend un ou plusieurs modules de source de lumière (1 '...1n), chaque module de source de lumière (1 '...1n) comprenant un support de montage de diodes électroluminescentes (2'...2n ; 11 '...11"), sur lequel un réseau de diodes électroluminescentes (3'...3n) sont montées, et qui est monté à son tour sur un support de montage de module (9, 9'), chacune desdites diodes électroluminescentes (3'...3n) étant apte à émettre un faisceau de lumière à une plage de longueurs d'onde (a, b, c), et en ce que lesdits moyens de filtrage comprennent un ou plusieurs éléments de filtre à sélection de bande d'excitation modulaires (6'...6n) chacun correspondant à l'un desdits modules de source de lumière (1...1 n), chaque élément de filtre à sélection de bande d'excitation (6'.. 6n) ayant une surface de réception de lumière et étant apte à recevoir le faisceau de lumière de chacune desdites diodes électroluminescentes (3'...3n) du module de source de lumière respectif (1'...1n) sur une partie correspondante de ladite surface de réception de lumière, de façon à choisir ainsi une bande de la plage de longueur d'onde de faisceau de lumière de chacune desdites diodes électroluminescentes (3'...3n).
PCT/IT2009/000225 2009-05-20 2009-05-20 Système d'excitation à fluorescence multibande modulaire WO2010134104A1 (fr)

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Cited By (5)

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WO2014015020A2 (fr) * 2012-07-18 2014-01-23 CHEN, Chung Yung Procédé destiné à la stimulation de la croissance des plantes, appareils et procédés destinés au calcul de la quantité cumulative de lumière
EP2702394A1 (fr) * 2011-04-28 2014-03-05 Bio-Rad Laboratories, Inc. Tête de balayage à fluorescence avec détection multibande
EP2713154A1 (fr) * 2012-10-01 2014-04-02 Roche Diagniostics GmbH Module de source de lumière et instrument analytique pour analyse d'un échantillon
CN104703464A (zh) * 2013-07-17 2015-06-10 吴炎东 一种促进植物生长的方法与一种光量累积计算装置及方法
EP3227740B1 (fr) * 2014-12-04 2024-02-14 ChemoMetec A/S Cytomètre d'image

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WO2009001390A1 (fr) * 2007-06-28 2008-12-31 Gian Luca Ferri Système de visualisation / imagerie et d'excitation multibande ajustable pour visualiser simultanément une fluorescence multiple

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Publication number Priority date Publication date Assignee Title
US6852986B1 (en) * 1999-11-12 2005-02-08 E. I. Du Pont De Nemours And Company Fluorometer with low heat-generating light source
JP2004212469A (ja) * 2002-12-27 2004-07-29 Hayashi Soken:Kk 照明装置およびそれを用いた顕微鏡
US20080188725A1 (en) * 2007-02-06 2008-08-07 Markle David R Optical systems and methods for ratiometric measurement of blood glucose concentration
WO2009001390A1 (fr) * 2007-06-28 2008-12-31 Gian Luca Ferri Système de visualisation / imagerie et d'excitation multibande ajustable pour visualiser simultanément une fluorescence multiple

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2702394A1 (fr) * 2011-04-28 2014-03-05 Bio-Rad Laboratories, Inc. Tête de balayage à fluorescence avec détection multibande
EP2702394A4 (fr) * 2011-04-28 2014-11-12 Bio Rad Laboratories Tête de balayage à fluorescence avec détection multibande
US9329127B2 (en) 2011-04-28 2016-05-03 Bio-Rad Laboratories, Inc. Fluorescence scanning head with multiband detection
WO2014015020A2 (fr) * 2012-07-18 2014-01-23 CHEN, Chung Yung Procédé destiné à la stimulation de la croissance des plantes, appareils et procédés destinés au calcul de la quantité cumulative de lumière
WO2014015020A3 (fr) * 2012-07-18 2015-04-02 CHEN, Chung Yung Procédé destiné à la stimulation de la croissance des plantes, appareils et procédés destinés au calcul de la quantité cumulative de lumière
EP2713154A1 (fr) * 2012-10-01 2014-04-02 Roche Diagniostics GmbH Module de source de lumière et instrument analytique pour analyse d'un échantillon
CN103712125A (zh) * 2012-10-01 2014-04-09 霍夫曼-拉罗奇有限公司 光源模块以及分析样品的分析仪器
CN107883204A (zh) * 2012-10-01 2018-04-06 霍夫曼-拉罗奇有限公司 光源模块以及分析样品的分析仪器
CN104703464A (zh) * 2013-07-17 2015-06-10 吴炎东 一种促进植物生长的方法与一种光量累积计算装置及方法
EP3227740B1 (fr) * 2014-12-04 2024-02-14 ChemoMetec A/S Cytomètre d'image

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