WO2010130307A8 - Method for detaching adherent cells - Google Patents

Method for detaching adherent cells Download PDF

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Publication number
WO2010130307A8
WO2010130307A8 PCT/EP2010/001082 EP2010001082W WO2010130307A8 WO 2010130307 A8 WO2010130307 A8 WO 2010130307A8 EP 2010001082 W EP2010001082 W EP 2010001082W WO 2010130307 A8 WO2010130307 A8 WO 2010130307A8
Authority
WO
WIPO (PCT)
Prior art keywords
adherent cells
methods
cells
detaching
detaching adherent
Prior art date
Application number
PCT/EP2010/001082
Other languages
French (fr)
Other versions
WO2010130307A1 (en
Inventor
Christian Derichs
Ulrike Koropp
Heike Walles
Michaela Kaufmann
Jan Hansmann
Original Assignee
Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. filed Critical Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V.
Priority to EP10705814A priority Critical patent/EP2430147A1/en
Publication of WO2010130307A1 publication Critical patent/WO2010130307A1/en
Publication of WO2010130307A8 publication Critical patent/WO2010130307A8/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/02—Membranes; Filters
    • C12M25/04—Membranes; Filters in combination with well or multiwell plates, i.e. culture inserts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention relates to methods for the cultivation of adherent cells, in particular for detaching the cells from a surface, to the use of membranes in these methods and to devices for carrying out the methods. A particular area of application is the use of the methods according to the invention in the GMP-compliant, fully automatic cultivation and propagation of cells.
PCT/EP2010/001082 2009-05-15 2010-02-20 Method for detaching adherent cells WO2010130307A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP10705814A EP2430147A1 (en) 2009-05-15 2010-02-20 Method for detaching adherent cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102009022352.5 2009-05-15
DE200910022352 DE102009022352A1 (en) 2009-05-15 2009-05-15 Removal procedure for adherent cells

Publications (2)

Publication Number Publication Date
WO2010130307A1 WO2010130307A1 (en) 2010-11-18
WO2010130307A8 true WO2010130307A8 (en) 2011-03-17

Family

ID=42094145

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2010/001082 WO2010130307A1 (en) 2009-05-15 2010-02-20 Method for detaching adherent cells

Country Status (3)

Country Link
EP (1) EP2430147A1 (en)
DE (1) DE102009022352A1 (en)
WO (1) WO2010130307A1 (en)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10240787B4 (en) 2002-08-30 2004-07-22 Oxyphen Ag Cell culture insert
CN1300297C (en) * 2002-09-20 2007-02-14 华东理工大学 Digestive reactor for animal cell extension inoculation
DE112007003608A5 (en) * 2007-08-09 2010-06-10 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Device and method for detaching cells

Also Published As

Publication number Publication date
EP2430147A1 (en) 2012-03-21
WO2010130307A1 (en) 2010-11-18
DE102009022352A1 (en) 2010-11-18

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