WO2010129265A3 - Compositions comprising monomeric streptavidin and methods for using same - Google Patents

Compositions comprising monomeric streptavidin and methods for using same Download PDF

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Publication number
WO2010129265A3
WO2010129265A3 PCT/US2010/032542 US2010032542W WO2010129265A3 WO 2010129265 A3 WO2010129265 A3 WO 2010129265A3 US 2010032542 W US2010032542 W US 2010032542W WO 2010129265 A3 WO2010129265 A3 WO 2010129265A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
compositions
same
contain
streptavidin proteins
Prior art date
Application number
PCT/US2010/032542
Other languages
French (fr)
Other versions
WO2010129265A2 (en
Inventor
Sheldon Park
Kok Hong Lim
Original Assignee
The Research Foundation Of State University Of New York
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The Research Foundation Of State University Of New York filed Critical The Research Foundation Of State University Of New York
Publication of WO2010129265A2 publication Critical patent/WO2010129265A2/en
Publication of WO2010129265A3 publication Critical patent/WO2010129265A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)

Abstract

Provided are improved streptavidin proteins and methods for using them. The improved streptavidin proteins can contain insertions, deletions and amino acid replacements, and contain at least one engineered disulfide-forming pair of amino acids. Also provided are method of the mutant streptavidin proteins to isolate biotinylated molecules, and complexes that contain mutant streptavidin proteins and biotinylated molecules.
PCT/US2010/032542 2009-04-27 2010-04-27 Compositions comprising monomeric streptavidin and methods for using same WO2010129265A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US17299609P 2009-04-27 2009-04-27
US61/172,996 2009-04-27

Publications (2)

Publication Number Publication Date
WO2010129265A2 WO2010129265A2 (en) 2010-11-11
WO2010129265A3 true WO2010129265A3 (en) 2011-03-03

Family

ID=43050717

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2010/032542 WO2010129265A2 (en) 2009-04-27 2010-04-27 Compositions comprising monomeric streptavidin and methods for using same

Country Status (1)

Country Link
WO (1) WO2010129265A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10759835B2 (en) 2016-07-25 2020-09-01 The Research Foundation For The State University Of New York Monomeric streptavidin mutants, methods of using the same and processes of manufacturing proteins
US11117936B2 (en) 2017-11-10 2021-09-14 University of Pittsburg—Of the Commonwealth System of Higher Education Affinity-enhanced monomeric streptavidin chimeric antigen receptor (CAR)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020177241A1 (en) * 2000-08-24 2002-11-28 Qinwei Shi Differential immunoassay
US20030095977A1 (en) * 1999-06-07 2003-05-22 Neorx Corporation Streptavidin expressed gene fusions and methods of use thereof
US20030215922A1 (en) * 1991-10-21 2003-11-20 Takeshi Sano Streptavidin proteins
US6972350B1 (en) * 1998-07-15 2005-12-06 The Horticulture And Food Research Institute Of New Zealand Pest-resistant plants comprising a construct encoding a vacuole targeting sequence and avidin or streptavidin
US20060246519A1 (en) * 2005-02-11 2006-11-02 Wu Sau-Ching Monomeric streptavidin muteins

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030215922A1 (en) * 1991-10-21 2003-11-20 Takeshi Sano Streptavidin proteins
US6972350B1 (en) * 1998-07-15 2005-12-06 The Horticulture And Food Research Institute Of New Zealand Pest-resistant plants comprising a construct encoding a vacuole targeting sequence and avidin or streptavidin
US20030095977A1 (en) * 1999-06-07 2003-05-22 Neorx Corporation Streptavidin expressed gene fusions and methods of use thereof
US20020177241A1 (en) * 2000-08-24 2002-11-28 Qinwei Shi Differential immunoassay
US20060246519A1 (en) * 2005-02-11 2006-11-02 Wu Sau-Ching Monomeric streptavidin muteins

Also Published As

Publication number Publication date
WO2010129265A2 (en) 2010-11-11

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