WO2010127303A1

WO2010127303A1 - Organisms for the production of isopropanol, n-butanol, and isobutanol

Info

Publication number: WO2010127303A1
Application number: PCT/US2010/033271
Authority: WO
Inventors: Anthony P. Burgard
Original assignee: Genomatica, Inc.
Priority date: 2009-04-30
Filing date: 2010-04-30
Publication date: 2010-11-04
Also published as: EP2425006A4; KR20120068763A; US20100323418A1; AU2010242849A1; EP2425006A1; US8993285B2; BRPI1013505A2; JP2012525156A

Abstract

A non-naturally occurring microbial organism having an isopropanol pathway includes at least one exogenous nucleic acid encoding an isopropanol pathway enzyme. The pathway includes an enzyme selected from a 4-hydroxybutyryl-CoA dehydratase, a crotonase, a 3 -hydro xybutyryl- CoA dehydrogenase, an acetoacetyl-CoA synthetase, an acetyl-CoA:acetoacetate-CoA transferase, an acetoacetyl-CoA hydrolase, an acetoacetate decarboxylase, and an acetone reductase. A non-naturally occurring microbial organism having an w-butanol pathway includes at least one exogenous nucleic acid encoding an w-butanol pathway enzyme. Other non- naturally occurring microbial organism have w-butanol or isobutanol pathways. The organisms are cultured to produce isopropanol, w-butanol, or isobutanol.

Description

ORGANISMS FOR THE PRODUCTION OF ISOPROPANOL. /i-BUTANOL. AND

ISOBUTANOL

STATEMENT OF RELATED APPLICATIONS This application claims the benefit of priority of U.S. Provisional Application No. 61/174,482, filed April 30, 2009, the entire contents of which are incorporated herein by reference.

BACKGROUND OF THE INVENTION

The present invention relates generally to biosynthetic processes and organisms capable of producing organic compounds. More specifically, the invention relates to non-naturally occurring organisms that can produce the commodity chemicals isopropanol, w-butanol, or isobutanol.

Isopropanol is a colorless, flammable, three-carbon alcohol that mixes completely with most solvents, including water. The largest use for isopropanol is as a solvent, including its well known yet small use as "rubbing alcohol," which is a mixture of isopropanol and water. As a solvent, isopropanol is found in many everyday products such as paints, lacquers, thinners, inks, adhesives, general-purpose cleaners, disinfectants, cosmetics, toiletries, de-icers, and pharmaceuticals. Low-grade isopropanol is also used in motor oils. The second largest use is as a chemical intermediate for the production of isopropylamines (e.g. in agricultural products), isopropylethers, and isopropyl esters. Isopropanol is manufactured by two petrochemical routes. The predominant process entails the hydration of propylene either with or without sulfuric acid catalysis. Secondarily, isopropanol is produced via hydrogenation of acetone, which is a byproduct formed in the production of phenol and propylene oxide. High-priced propylene is currently driving costs up and margins down throughout the chemical industry motivating the need for an expanded range of low cost feedstocks.

Butanol, or equivalently, w-butanol, is a four carbon alcohol that is currently manufactured almost exclusively through the use of petrochemical raw materials. The main petrochemical process entails carbonylation of propylene to butyraldehyde, followed by catalytic hydrogenation to butanol. The demand for butanol is driven by its use for production of butylacrylate and butyl methacrylate, both of which are employed in emulsified and solution polymers used in water-based latex coatings, enamels, and lacquers. Other application include its use as an intermediate for large volume chemicals such as butyl acetate and glycol butyl ethers, as well as it direct use as a solvent. Butanol also is being considered for potential application as a biofuel derived from renewable resources. Butanol has a wide range of properties that make it better suited as a fuel than ethanol. For example, butanol has higher energy content, lower volatility and hygroscopicity, can be shipped through pipeline infrastructure, can be used directly without blending, and can be blended with diesel or biodiesel.

Isobutanol is another colorless, flammable, four carbon alcohol that is being aggressively pursued as a biofuel. Currently, its major application is as a starting material for isobutyl acetate, a common solvent used in the production of lacquer and coatings and also as a flavoring agent in the food industry. Isobutyl esters are used in plastics, rubbers, and other dispersions. Additional applications for isobutanol include its use as a solvent in paint, varnish removers, and inks. Methods for isobutanol synthesis from petroleum derived feedstocks include oxo synthesis (Weber et al., Industrial & Engineering Chemistry Research, 62:33-37 (1970)) and Guerbet condensation of methanol with w-propanol (Carlini et al., J. of Molecular Catalysis A: Chemical, 220:215-220 (2004);Carlini et al., J. of Molecular Catalysis A: Chemical, 184:273-280 (2002);Carlini et al., J. of Molecular Catalysis A: Chemical, 200:137-146 (2003);Carlini et al., J. of Molecular Catalysis A: Chemical, 206:409-418 (2003)).

Thus, there exists a need to develop microorganisms and methods of their use to produce isopropanol, w-butanol, or isobutanol using low cost feedstocks. The present invention satisfies this need and provides related advantages as well.

SUMMARY OF THE INVENTION

In some embodiments, the present invention provides a non-naturally occurring microbial organism that includes a microbial organism having an isopropanol pathway having at least one exogenous nucleic acid encoding an isopropanol pathway enzyme expressed in a sufficient amount to produce isopropanol. The isopropanol pathway includes an enzyme selected from the group consisting of a 4-hydroxybutyryl-CoA dehydratase, a crotonase, a 3 -hydroxybutyryl-CoA dehydrogenase, an acetoacetyl-CoA synthetase, an acetyl-CoA:acetoacetate-CoA transferase, an acetoacetyl-CoA hydrolase, an acetoacetate decarboxylase, and an acetone reductase.

In other embodiments, the present invention provides a method for producing isopropanol that includes culturing such a non-naturally occurring microbial organism having an isopropanol pathway under conditions and for a sufficient period of time to produce isopropanol. In some embodiments, the present invention provides a non-naturally occurring microbial organism that includes a microbial organism having a w-butanol pathway having at least one exogenous nucleic acid encoding a w-butanol pathway enzyme expressed in a sufficient amount to produce w-butanol. The w-butanol pathway comprising an enzyme selected from the group consisting of a 4-hydroxybutyryl-CoA dehydratase, a crotonoyl-CoA reductase, a butyryl-CoA reductase (aldehyde forming), a butyraldehyde reductase, and a butyryl-CoA reductase (alcohol forming).

In some embodiments, the present invention provides a method for producing w-butanol comprising culturing a non-naturally occurring microbial organism having an w-butanol pathway, under conditions and for a sufficient period of time to produce w-butanol.

In some embodiments, the present invention provides a non-naturally occurring microbial organism that includes a microbial organism having an isobutanol pathway having at least one exogenous nucleic acid encoding an isobutanol pathway enzyme expressed in a sufficient amount to produce isobutanol. The isobutanol pathway includes an enzyme selected from the group consisting of a 4-hydroxybutyryl-CoA dehydratase, a crotonoyl-CoA reductase, an isobutyryl-CoA mutase, a 4-hydroxybutyryl-CoA mutase, a 3-hydroxyisobutyryl-CoA dehydratase, a methacrylyl-CoA-reductase, an isobutyryl-CoA reductase (aldehyde forming), an isobutyraldehyde reductase, and an isobutyryl-CoA reductase (alcohol forming).

In some embodiments, the present invention provides a method for producing isobutanol that includes culturing a non-naturally occurring microbial organism having an isobutanol pathway, under conditions and for a sufficient period of time to produce isobutanol.

BRIEF DESCRIPTION OF THE DRAWINGS

Figure 1 shows pathways to isopropanol, w-butanol, and isobutanol from 4-hydroxybutyryl-CoA.

DETAILED DESCRIPTION OF THE INVENTION

This invention is directed, in part, to non-naturally occurring microorganisms that express genes encoding enzymes that catalyze isopropanol, «-butanol, or isobutanol production. Pathways for the production of isopropanol, w-butanol, or isobutanol disclosed herein are based on 4- hydroxybutyryl-CoA as a starting material as shown in Figure 1. Successfully engineering these pathways entails identifying an appropriate set of enzymes with sufficient activity and specificity, cloning their corresponding genes into a production host, optimizing fermentation conditions, and assaying for product formation following fermentation.

Although 4-hydroxybutyryl-CoA is not a highly common central metabolite, methods for engineering strains that synthesize 4-hydroxybutyryl-CoA have been described previously by Applicants in U.S. Patent Application No. 2009/0075351, which is incorporated by reference herein in its entirety. An exemplary method involves synthesizing 4-hydroxybutyryl-CoA from succinyl-CoA by employing genes encoding succinic semialdehyde dehydrogenase (CoA- dependent), 4-hydroxybutyrate dehydrogenase, 4-hydroxybutyrate kinase, and phosphotransbutyrylase activities.

Assuming glucose as the carbohydrate feedstock, synthesizing isopropanol from 4- hydroxybutyryl-CoA has a theoretical yield of 1.33 mol/mol. This is 33% higher than the maximum yield attainable using the pathway described by Subbian, et al. in U.S. 2008/0293125. Another benefit of the 4-hydroxybutyryl-CoA to isopropanol pathway is that it can enable redox balance in the absence of an external electron acceptor, whereas the pathway described in Subbian, et al. produces a surplus of NADH that must be dissipated by transferring electrons to an external electron acceptor.

Another compound that can be synthesized from 4-hydroxybutyryl-CoA is w-butanol. Assuming glucose as a carbohydrate feedstock, this pathway has a theoretical yield of about 1.00 mol/mol yield of w-butanol. This yield is comparable to a route to w-butanol, native to many Clostridial species, that involves the formation of acetoacetyl-CoA from acetyl-CoA, followed by four reductions and a dehydration (Jones et al., Microbiol. Rev., 50:484-524 (1986)). A benefit of the present invention is that it bypasses the first three steps of this traditional butanol production pathway (i.e., acetyl-CoA acetyltransferase, 3-hydroxybutyryl-CoA dehydrogenase, and crotonase) which form one molecule of crotonoyl-CoA from two acetyl-CoA molecules. Any or all of these enzymes represent potential bottlenecks to production. For example, although recombinant strains of E. coli have been engineered to express the requisite Clostridial genes for the traditional butanol synthetic pathway, titers of less than 1 g/L were reported (Atsumi, et al, Metabolic Engineering, 2008, 10, 305-311). Further benefits of the current invention are found in the underlying central metabolism required to produce w-butanol from 4-hydroxybutyryl-CoA as opposed to producing w-butanol from two acetyl-CoA molecules. Specifically, to establish the highest yield and redox balance under anaerobic conditions, the traditional Clostridial route requires that two reducing equivalents per w-butanol are extracted from the conversion of pyruvate to acetyl-CoA (i.e., two pyruvate molecules must be oxidized to two acetyl-CoA molecules per butanol produced). This represents a challenge in organisms such as Escherichia coli, in which pyruvate dehydrogenase is not naturally highly active under anaerobic or microaerobic conditions. The production pathway disclosed herein needs only one pyruvate to be oxidized to acetyl-CoA per w-butanol produced. The additional reducing equivalent is generated by the conversion of isocitrate to alpha-ketoglutarate by isocitrate dehydrogenase.

Similarly, the theoretical yield of isobutanol via the 4-hydroxybutyryl-CoA pathway is about 1.00 mol/mol assuming glucose as the feedstock. One benefit of the current invention is that it bypasses the acetolactate synthase, acetohydroxy acid isomeroreductase, acetohydroxy acid dehydratase, and branched chain alpha-keto acid dehydrogenase steps of the conversion pathway from pyruvate to isobutyryl-CoA to isobutanol described in Donaldson et al., U.S 20070092957. Alternatively, it bypasses the acetyl-CoA acetyltransferase, 3-hydroxybutyryl-CoA dehydrogenase, and crotonase steps required for the production of butyryl-CoA and isobutanol via another biosynthetic pathway also described in Donaldson, et al. Any or all of these enzymatic steps bypassed by the current invention represent potential bottlenecks to isobutanol production. In one embodiment of the present invention, the butyryl-CoA intermediate is bypassed completely by converting 4-hydroxybutyryl-CoA to 3-hydroxyisobutyryl-CoA, which is subsequently dehydrated and reduced to isobutyryl-CoA.

Finally, this invention is also directed, in part, to methods for producing isopropanol, w-butanol, or isobutanol through culturing of these no n- naturally occurring microbial organisms. Thus, any of the strains disclosed herein can be cultured under appropriate conditions, for a sufficient period of time to provide the commodity chemicals isopropanol, w-butanol, or isobutanol.

As used herein, the term "non-naturally occurring" when used in reference to a microbial organism or microorganism of the invention is intended to mean that the microbial organism has at least one genetic alteration not normally found in a naturally occurring strain of the referenced species, including wild-type strains of the referenced species. Genetic alterations include, for example, modifications introducing expressible nucleic acids encoding metabolic polypeptides, other nucleic acid additions, nucleic acid deletions and/or other functional disruption of the microbial genetic material. Such modifications include, for example, coding regions and functional fragments thereof, for heterologous, homologous or both heterologous and homologous polypeptides for the referenced species. Additional modifications include, for example, non-coding regulatory regions in which the modifications alter expression of a gene or operon. Exemplary metabolic polypeptides include enzymes or proteins within an isopropanol, M-butanol, or isobutanol biosynthetic pathway. A metabolic modification refers to a biochemical reaction that is altered from its naturally occurring state. Therefore, non-naturally occurring microorganisms can have genetic modifications to nucleic acids encoding metabolic polypeptides or, functional fragments thereof. Exemplary metabolic modifications are disclosed herein.

As used herein, the term "isolated" when used in reference to a microbial organism is intended to mean an organism that is substantially free of at least one component as the referenced microbial organism is found in nature. The term includes a microbial organism that is removed from some or all components as it is found in its natural environment. The term also includes a microbial organism that is removed from some or all components as the microbial organism is found in non-naturally occurring environments. Therefore, an isolated microbial organism is partly or completely separated from other substances as it is found in nature or as it is grown, stored or subsisted in non-naturally occurring environments. Specific examples of isolated microbial organisms include partially pure microbes, substantially pure microbes and microbes cultured in a medium that is non-naturally occurring.

As used herein, the terms "microbial," "microbial organism" or "microorganism" is intended to mean any organism that exists as a microscopic cell that is included within the domains of archaea, bacteria or eukarya. Therefore, the term is intended to encompass prokaryotic or eukaryotic cells or organisms having a microscopic size and includes bacteria, archaea and eubacteria of all species as well as eukaryotic microorganisms such as yeast and fungi. The term also includes cell cultures of any species that can be cultured for the production of a biochemical.

As used herein, the term "CoA" or "coenzyme A" is intended to mean an organic cofactor or prosthetic group (nonprotein portion of an enzyme) whose presence is required for the activity of many enzymes (the apoenzyme) to form an active enzyme system. Coenzyme A functions in certain condensing enzymes, acts in acetyl or other acyl group transfer and in fatty acid synthesis and oxidation, pyruvate oxidation and in other acetylation.

As used herein, the term "substantially anaerobic" when used in reference to a culture or growth condition is intended to mean that the amount of oxygen is less than about 10% of saturation for dissolved oxygen in liquid media. The term also is intended to include sealed chambers of liquid or solid medium maintained with an atmosphere of less than about 1% oxygen. "Exogenous" as it is used herein is intended to mean that the referenced molecule or the referenced activity is introduced into the host microbial organism. The molecule can be introduced, for example, by introduction of an encoding nucleic acid into the host genetic material such as by integration into a host chromosome or as non-chromosomal genetic material such as a plasmid. Therefore, the term as it is used in reference to expression of an encoding nucleic acid refers to introduction of the encoding nucleic acid in an expressible form into the microbial organism. When used in reference to a biosynthetic activity, the term refers to an activity that is introduced into the host reference organism. The source can be, for example, a homologous or heterologous encoding nucleic acid that expresses the referenced activity following introduction into the host microbial organism. Therefore, the term "endogenous" refers to a referenced molecule or activity that is present in the host. Similarly, the term when used in reference to expression of an encoding nucleic acid refers to expression of an encoding nucleic acid contained within the microbial organism. The term "heterologous" refers to a molecule or activity derived from a source other than the referenced species whereas "homologous" refers to a molecule or activity derived from the host microbial organism. Accordingly, exogenous expression of an encoding nucleic acid of the invention can utilize either or both a heterologous or homologous encoding nucleic acid.

The no n- naturally occurring microbal organisms of the invention can contain stable genetic alterations, which refers to microorganisms that can be cultured for greater than five generations without loss of the alteration. Generally, stable genetic alterations include modifications that persist greater than 10 generations, particularly stable modifications will persist more than about 25 generations, and more particularly, stable genetic modifications will be greater than 50 generations, including indefinitely.

Those skilled in the art will understand that the genetic alterations, including metabolic modifications exemplified herein, are described with reference to a suitable host organism such as E. coli and their corresponding metabolic reactions or a suitable source organism for desired genetic material such as genes for a desired metabolic pathway. However, given the complete genome sequencing of a wide variety of organisms and the high level of skill in the area of genomics, those skilled in the art will readily be able to apply the teachings and guidance provided herein to essentially all other organisms. For example, the E. coli metabolic alterations exemplified herein can readily be applied to other species by incorporating the same or analogous encoding nucleic acid from species other than the referenced species. Such genetic alterations include, for example, genetic alterations of species homologs, in general, and in particular, orthologs, paralogs or nonorthologous gene displacements.

An ortholog is a gene or genes that are related by vertical descent and are responsible for substantially the same or identical functions in different organisms. For example, mouse epoxide hydrolase and human epoxide hydrolase can be considered orthologs for the biological function of hydrolysis of epoxides. Genes are related by vertical descent when, for example, they share sequence similarity of sufficient amount to indicate they are homologous, or related by evolution from a common ancestor. Genes can also be considered orthologs if they share three-dimensional structure but not necessarily sequence similarity, of a sufficient amount to indicate that they have evolved from a common ancestor to the extent that the primary sequence similarity is not identifiable. Genes that are orthologous can encode proteins with sequence similarity of about 25% to 100% amino acid sequence identity. Genes encoding proteins sharing an amino acid similarity less that 25% can also be considered to have arisen by vertical descent if their three-dimensional structure also shows similarities. Members of the serine protease family of enzymes, including tissue plasminogen activator and elastase, are considered to have arisen by vertical descent from a common ancestor.

Orthologs include genes or their encoded gene products that through, for example, evolution, have diverged in structure or overall activity. For example, where one species encodes a gene product exhibiting two functions and where such functions have been separated into distinct genes in a second species, the three genes and their corresponding products are considered to be orthologs. For the production of a biochemical product, those skilled in the art will understand that the orthologous gene harboring the metabolic activity to be introduced or disrupted is to be chosen for construction of the non-naturally occurring microorganism. An example of orthologs exhibiting separable activities is where distinct activities have been separated into distinct gene products between two or more species or within a single species. A specific example is the separation of elastase proteolysis and plasminogen proteolysis, two types of serine protease activity, into distinct molecules as plasminogen activator and elastase. A second example is the separation of mycoplasma 5 '-3' exo nuclease and Drosophila DNA polymerase III activity. The DNA polymerase from the first species can be considered an ortholog to either or both of the exo nuclease or the polymerase from the second species and vice versa.

In contrast, paralogs are homologs related by, for example, duplication followed by evolutionary divergence and have similar or common, but not identical functions. Paralogs can originate or derive from, for example, the same species or from a different species. For example, microsomal epoxide hydrolase (epoxide hydrolase I) and soluble epoxide hydrolase (epoxide hydrolase II) can be considered paralogs because they represent two distinct enzymes, co- evolved from a common ancestor, that catalyze distinct reactions and have distinct functions in the same species. Paralogs are proteins from the same species with significant sequence similarity to each other indicating that they are homologous, or related through co-evolution from a common ancestor. Groups of paralogous protein families include HipA homologs, luciferase genes, peptidases, and others.

A nonorthologous gene displacement is a nonorthologous gene from one species that can substitute for a referenced gene function in a different species. Substitution includes, for example, being able to perform substantially the same or a similar function in the species of origin compared to the referenced function in the different species. Although generally, a nonorthologous gene displacement will be identifiable as structurally related to a known gene encoding the referenced function, less structurally related but functionally similar genes and their corresponding gene products nevertheless will still fall within the meaning of the term as it is used herein. Functional similarity requires, for example, at least some structural similarity in the active site or binding region of a nonorthologous gene product compared to a gene encoding the function sought to be substituted. Therefore, a nonorthologous gene includes, for example, a paralog or an unrelated gene.

Therefore, in identifying and constructing the non-naturally occurring microbial organisms of the invention having isopropanol, «-butanol, or isobutanol biosynthetic capability, those skilled in the art will understand with applying the teaching and guidance provided herein to a particular species that the identification of metabolic modifications can include identification and inclusion or inactivation of orthologs. To the extent that paralogs and/or nonorthologous gene displacements are present in the referenced microorganism that encode an enzyme catalyzing a similar or substantially similar metabolic reaction, those skilled in the art also can utilize these evolutionally related genes.

Orthologs, paralogs and nonorthologous gene displacements can be determined by methods well known to those skilled in the art. For example, inspection of nucleic acid or amino acid sequences for two polypeptides will reveal sequence identity and similarities between the compared sequences. Based on such similarities, one skilled in the art can determine if the similarity is sufficiently high to indicate the proteins are related through evolution from a common ancestor. Algorithms well known to those skilled in the art, such as Align, BLAST, Clustal W and others compare and determine a raw sequence similarity or identity, and also determine the presence or significance of gaps in the sequence which can be assigned a weight or score. Such algorithms also are known in the art and are similarly applicable for determining nucleotide sequence similarity or identity. Parameters for sufficient similarity to determine relatedness are computed based on well known methods for calculating statistical similarity, or the chance of finding a similar match in a random polypeptide, and the significance of the match determined. A computer comparison of two or more sequences can, if desired, also be optimized visually by those skilled in the art. Related gene products or proteins can be expected to have a high similarity, for example, 25% to 100% sequence identity. Proteins that are unrelated can have an identity which is essentially the same as would be expected to occur by chance, if a database of sufficient size is scanned (about 5%). Sequences between 5% and 24% may or may not represent sufficient homology to conclude that the compared sequences are related. Additional statistical analysis to determine the significance of such matches given the size of the data set can be carried out to determine the relevance of these sequences.

Exemplary parameters for determining relatedness of two or more sequences using the BLAST algorithm, for example, can be as set forth below. Briefly, amino acid sequence alignments can be performed using BLASTP version 2.0.8 (Jan-05-1999) and the following parameters: Matrix: 0 BLOSUM 62; gap open: 11; gap extension: 1; x_dropoff: 50; expect: 10.0; wordsize: 3; filter: on. Nucleic acid sequence alignments can be performed using BLASTN version 2.0.6 (Sept-16-1998) and the following parameters: Match: 1 ; mismatch: -2; gap open: 5; gap extension: 2; x_dropoff: 50; expect: 10.0; wordsize: 11; filter: off. Those skilled in the art will know what modifications can be made to the above parameters to either increase or decrease the stringency of the comparison, for example, and determine the relatedness of two or more sequences.

In some embodiments, the microbial organism includes two exogenous nucleic acids, each encoding an isopropanol pathway enzyme, while in other embodiments the microbial organism includes three exogenous nucleic acids, each encoding an isopropanol pathway enzyme. In some embodiments, the microbial organism includes four exogenous nucleic acids, each encoding an isopropanol pathway enzyme. In further embodiments, the microbial organism includes five exogenous nucleic acids, each encoding an isopropanol pathway enzyme. In yet further embodiments, the microbial organism includes six exogenous nucleic acids, each encoding an isopropanol pathway enzyme. The microbial organism can also include seven exogenous nucleic acids, each encoding an isopropanol pathway enzyme. Finally, the microbial organism can include eight exogenous nucleic acids, each encoding an isopropanol pathway enzyme. Any of the aforementioned genes that are inserted into the host organism can be a heterologous nucleic acid. In some embodiments, the non-naturally occurring microbial organism is in a substantially anaerobic culture medium.

In accordance with the foregoing, the present invention provides a 4-hydroxybutyryl-CoA to isopropanol pathway that provides a nucleic acid encoding an enzyme that carries out the dehydration of 4-hydroxybutyryl-CoA to form crotonoyl-CoA as shown in step A of Figure 1. Crotonase subsequently hydrates crotonoyl-CoA to 3-hydroxybutyryl-CoA (step B) which, in turn, is oxidized to acetoacetyl-CoA by 3 -hydro xybutyryl-CoA dehydrogenase (step C).

Acetoacetyl-CoA is converted to acetoacetate by a synthetase, transferase, or hydrolase (steps D, E, or F). The final two steps involve the decarboxylation of acetoacetate to form acetone (step G) and its subsequent reduction to isopropanol (step H).

In an additional embodiment, the invention provides a non-naturally occurring microbial organism having an isopropanol pathway, wherein the non-naturally occurring microbial organism comprises at least one exogenous nucleic acid encoding an enzyme or protein that converts a substrate to a product selected from the group consisting of 4-hydroxybutyryl-CoA to crotonoyl-CoA, crotonoyl-CoA to 3 -hydroxybutyryl-CoA, 3-hydroxybutyryl-CoA to acetoacetyl-CoA, acetoacetacetyl-CoA to acetoacetate, acetoacetate to acetone, and acetone to isopropanol.

In some embodiments, the present invention provides a non-naturally occurring microbial organism that includes a microbial organism having a w-butanol pathway having at least one exogenous nucleic acid encoding a w-butanol pathway enzyme expressed in a sufficient amount to produce w-butanol. The w-butanol pathway includes an enzyme selected from the group consisting of a 4-hydroxybutyryl-CoA dehydratase, a crotonoyl-CoA reductase, a butyryl-CoA reductase (aldehyde forming), a butyraldehyde reductase, and a butyryl-CoA reductase (alcohol forming). In some embodiments, the microbial organism includes two exogenous nucleic acids, each encoding an w-butanol pathway enzyme, while in other embodiments, the microbial organism includes three exogenous nucleic acids, each encoding an w-butanol pathway enzyme. In further embodiments, the microbial organism includes four exogenous nucleic acids, each encoding an M-butanol pathway enzyme. Any of the aforementioned nucleic acids can be provided as a heterologous nucleic acid. Such non-naturally occurring microbial organism can be grown in a substantially anaerobic culture medium.

In an additional embodiment, the invention provides a non-naturally occurring microbial organism having a w-butanol pathway, wherein the non-naturally occurring microbial organism comprises at least one exogenous nucleic acid encoding an enzyme or protein that converts a substrate to a product selected from the group consisting of 4-hydroxybutyryl-CoA to crotonoyl- CoA, crotonoyl-CoA to butyryl-CoA, butyryl-CoA to w-butanol, butyryl-CoA to butyraldehyde, and butyraldehyde to w-butanol.

The 4-hydroxybutyryl-CoA to w-butanol pathway begins with the dehydration of 4- hydroxybutyryl-CoA to crotonoyl-CoA as shown in step A of Figure 1, which is then reduced to butyryl-CoA (step I). Butyryl-CoA then undergoes two reductions carried out either by two separate enzymes, steps J and K, or a single dual-function enzyme as shown in step L.

In some embodiments, the microbial organism includes two exogenous nucleic acids, each encoding an isobutanol pathway enzyme, while in other embodiments, the microbial organism includes three exogenous nucleic acids, each encoding an isobutanol pathway enzyme. In other embodiments, the microbial organism includes four exogenous nucleic acids, each encoding an isobutanol pathway enzyme. In still further embodiments, the microbial organism includes five exogenous nucleic acids, each encoding an isobutanol pathway enzyme. Any of the exogenous nucleic acid can be a heterologous nucleic acid. Such non-naturally occurring microbial organism can be grown in a substantially anaerobic culture medium.

In some embodiments, the non-naturally occurring organism has a set of isobutanol pathway enzymes that includes a 4-hydroxybutyryl-CoA dehydratase, a crotonoyl-CoA reductase, an isobutyryl-CoA mutase, an isobutyryl-CoA reductase (aldehyde forming), and an isobutyraldehyde reductase. Such organisms can have one, two three, four, five, up to all nucleic acids encoding isobutanol pathway enzymes provided as exogenous nucleic acids.

In some embodiments, the non-naturally occurring organism has a set of isobutanol pathway enzymes comprises a 4-hydroxybutyryl-CoA dehydratase, a crotonoyl-CoA reductase, an isobutyryl-CoA mutase, and an isobutyryl-CoA reductase (alcohol forming). Such organisms can have one, two three, four, up to all nucleic acids encoding isobutanol pathway enzymes provided as exogenous nucleic acids.

In some embodiments, the non-naturally occurring organism has a set of isobutanol pathway enzymes comprises a 4-hydroxybutyryl-CoA mutase, a 3 -hydro xyisobutyryl-CoA dehydratase, a methacrylyl-CoA-reductase, an isobutyryl-CoA reductase (aldehyde forming), and an isobutyraldehyde reductase. Such organisms can have one, two three, four, five, up to all nucleic acids encoding isobutanol pathway enzymes provided as exogenous nucleic acids.

In some embodiments, the non-naturally occurring organism has a set of isobutanol pathway enzymes comprises a 4-hydroxybutyryl-CoA mutase, a 3 -hydro xyisobutyryl-CoA dehydratase, a methacrylyl-CoA-reductase, and an isobutyryl-CoA reductase (alcohol forming). Such organisms can have one, two three, four, up to all nucleic acids encoding isobutanol pathway enzymes provided as exogenous nucleic acids.

In an additional embodiment, the invention provides a non-naturally occurring microbial organism having an isobutanol pathway, wherein the non-naturally occurring microbial organism comprises at least one exogenous nucleic acid encoding an enzyme or protein that converts a substrate to a product selected from the group consisting of 4-hydroxybutyryl-CoA to crotonoyl-CoA, crotonoyl-CoA to butyryl-CoA, butyryl-CoA to isobutyryl-CoA, 4- hydroxybutyryl-CoA to 3-hydroxyisobutyryl-CoA, 3-hydroxyisobutyryl-CoA to methacrylyl- CoA, methacrylyl-CoA to isobutyryl-CoA, isobutyryl-CoA to isobutanol, isobutyryl-CoA to isobutyraldehyde, and isobutryaldehyde to isobutanol. The conversion of 4-hydroxybutyryl-CoA to isobutanol involves the formation of an isobutyryl- CoA intermediate. Isobutyryl-CoA can be formed from 4-hydroxybutyryl-CoA via a dehydration, reduction, and carbon backbone rearrangement as shown in steps A, I, and M, of Figure 1. Alternatively, this intermediate can be obtained via first carbon backbone rearrangement, then dehydration and reduction as shown in steps Q, R, and S of Figure 1.

Isobutyryl-CoA then undergoes two reductions to form isobutanol. The reductions are carried out either by two enzymes, steps N and O or a single dual- function enzyme as shown in step P.

Successfully engineering any of these pathways entails identifying an appropriate set of enzymes with sufficient activity and specificity, cloning their corresponding genes into a production host, optimizing fermentation conditions, and assaying for product formation following fermentation. To engineer a production host for the production of any of the aforementioned products, one or more exogenous DNA sequence(s) can be expressed in microorganisms. In addition, the microorganisms can have endogenous gene(s) functionally deleted. These modifications will enable the production of isopropanol, w-butanol, or isobutanol using renewable feedstocks.

Below, we describe a number of biochemically characterized genes capable of encoding enzymes that catalyze each of the steps shown in Figure 1. Although described in the context of an engineered E. coli, one skilled in the art can apply these teachings to essentially any other organism. Specifically, genes are listed that are native to E. coli as well as other genes in other organisms that can be applied to catalyze the appropriate transformations when properly cloned and expressed.

The invention is described herein with general reference to the metabolic reaction, reactant or product thereof, or with specific reference to one or more nucleic acids or genes encoding an enzyme associated with or catalyzing, or a protein associated with, the referenced metabolic reaction, reactant or product. Unless otherwise expressly stated herein, those skilled in the art will understand that reference to a reaction also constitutes reference to the reactants and products of the reaction. Similarly, unless otherwise expressly stated herein, reference to a reactant or product also references the reaction, and reference to any of these metabolic constituents also references the gene or genes encoding the enzymes that catalyze or proteins involved in the referenced reaction, reactant or product. Likewise, given the well known fields of metabolic biochemistry, enzymology and genomics, reference herein to a gene or encoding nucleic acid also constitutes a reference to the corresponding encoded enzyme and the reaction it catalyzes or a protein associated with the reaction as well as the reactants and products of the reaction.

All transformations depicted in Figure 1 fall into the 10 general categories of transformations shown in Table 1. Below is described a number of biochemically characterized genes in each category. Specifically listed are genes that can be applied to catalyze the appropriate transformations in Figure lwhen properly cloned and expressed. Exemplary genes for each of the steps in Figure 1 are provided further below in Table 37.

Table 1 shows the enzyme types useful to convert common central metabolic intermediates into isopropanol, «-butanol, or isobutanol. The first three digits of each label correspond to the first three Enzyme Commission number digits which denote the general type of transformation independent of substrate specificity.

Table 1

Two transformations in Figure 1 fall into the category of oxidoreductases that reduce an aldehyde to alcohol. Specifically, these are butyraldehyde reductase and isobutyraldehyde reductase, as shown in steps K and O, respectively.

Exemplary genes encoding enzymes that catalyze the conversion of an aldehyde to alcohol (i.e., alcohol dehydrogenase or equivalently aldehyde reductase) include air A encoding a medium- chain alcohol dehydrogenase for C2-C14 (Tani et al, Appl. Environ. Microdot, 66:5231-5235 (2000)), ADH2 from Saccharomyces cerevisiae (Atsumi et al., Nature, 451 :86-89 (2008)), jg/zD from E. coli which has preference for molecules longer than C3 (Sulzenbacher et al., J. of Molecular Biology, 342:489-502 (2004)), and bdh I and bdh II from C acetobutylicum which converts butyraldehyde into butanol (Walter et al., J. of Bacteriology, 174:7149-7158 (1992)). The gene product oϊyqhD catalyzes the reduction of acetaldehyde, malondialdehyde, propionaldehyde, butyraldehyde, and acrolein using NADPH as the cofactor ( Perez et al., J Biol. Chem., 283 :7346-7353 (2008)). ADHl from Zymomonas mobilis has been demonstrated to have activity on a number of aldehydes including formaldehyde, acetaldehyde, propionaldehyde, butyraldehyde, and acrolein (Kinoshita et al., Appl Microbiol Biotechnol, 22:249-254 (1985)). Data related to the sequences for each of these exemplary gene products can be found using the following GenBank accession numbers shown in Table 2.

Table 2

Enzymes exhibiting 3 -hydro xybutyraldehyde reductase activity (EC 1.1.1.61) also fall into this category. Such enzymes have been characterized in Ralstonia eutropha Bravo et al., J. Forensic ScL, 49:379-387(2004)), Clostridium kluyveri (Wolff et al, Protein Expr. Purif, 6:206-212(1995)) and Arabidopsis thaliana ( Breitkreuz et al., J. Biol. Chem., 278:41552-41556 (2003)). Yet another gene is the alcohol dehydrogenase adhl from Geobacillus thermoglucosidasius ( Jeon et al., J Biotechnol, 135:127-133 (2008)). Data related to the sequences for each of these exemplary gene products can be found using the following GenBank accession numbers shown in Table 3.

Table 3

Another exemplary enzyme is 3 -hydro xyisobutyrate dehydrogenase which catalyzes the reversible oxidation of 3 -hydro xyisobutyrate to methylmalonate semialdehyde. This enzyme participates in valine, leucine and isoleucine degradation and has been identified in bacteria, eukaryotes, and mammals. The enzyme encoded by P84067 from Thermus thermophilus HB8 has been structurally characterized (Lokanath et al., JMoI Biol, 352:905-917 (2005)). The reversibility of the human 3 -hydro xyisobutyrate dehydrogenase was demonstrated using isotopically- labeled substrate ( Manning et al., Biochem J, 231 :481-484 (1985)). Additional genes encoding this enzyme include 3hidh in Homo sapiens ( Hawes et al., Methods Enzymol, 324:218-228 (2000)) and Oryctolagus cuniculus (Hawes et al, supra; Chowdhury et al., Biosci. Biotechnol Biochem. , 60:2043-2047 (1996)), mmsb in Pseudomonas aeruginosa, and dhat in Pseudomonas putida (Hawes et al, supra; Chowdhury et al., Biosci. Biotechnol Biochem., 60:2043-2047 (1996); Chowdhury et al., Biosci. Biotechnol Biochem. , 67:438-441(2003)). Data related to the sequences for each of these exemplary gene products can be found using the following GenBank accession numbers shown in Table 4.

Table 4

Oxidoreductases that convert a ketone functionality to the corresponding hydroxyl group are exemplified by step H, for the conversion of acetone to isopropanol, and by step C for the conversion of 3-hydroxybutyryl-CoA to acetoacetyl-CoA as shown in Figure 1. An exemplary alcohol dehydrogenase that converts acetone to isopropanol was shown in C. beijerinckii ( Ismaiel et al., J. Bacteriol, 175:5097-5105(1993)) and T. brockii (Lamed et al., Biochem. J., 195: 183-190(1981); Peretz et al., Biochemistry, 28:6549-6555 (1989)). The gene product of adhA from Pyrococcus furiosus, which exhibits maximum activity on 2-pentanol and pyruvaldehyde, was shown to have very broad specificity which includes isopropanol and acetone (van der et al., Eur J Biochem, 268:3062-3068 (2001)). Yet another secondary alcohol dehydrogenase with activity on isopropanol and acetone is encoded by the gene product oiadh- A from Rhodococcus ruber (Kosjek et al., Biotechnol Bioeng, 86:55-62 (2004)). These genes along with others are listed below in Table 5. Table 5

3-hydroxybutyryl-CoA dehydrogenase (Step C) catalyzing the formation of acetoacetyl-CoA from 3-hydroxybutyryl-CoA participates in the acetyl-CoA fermentation pathway to butyrate in several species of Clostridia and has been studied in detail (Jones and Woods, Microbiol. Rev. 50:484-524 (1986). The enzyme from Clostridium acetobutylicum, encoded by hbd, has been cloned and functionally expressed in is. coli (Youngleson et al., J. Bacteriol. 171 :6800-6807 (1989). Additionally, subunits of two fatty acid oxidation complexes in is. coli, encoded by fadB and fadJ, function as 3-hydroxyacyl-CoA dehydrogenases (Binstock and Schulz, Meth. Enzymol. 71 Pt C, 403-411 (1981). Yet other genes demonstrated to catalyze this reversible transformation are phbB from Zoogloea ramigera (Ploux et al., Eur. J. Biochem. 174:177-182 (1988) and phaB from Rhodobacter sphaeroides (Alber et al., MoI. Microbiol. 61 :297-309 (2006). The former gene is NADPH-dependent, its nucleotide sequence has been determined (Peoples and Sinskey, MoI. Microbiol. 3:349-257 (1989), and the gene has been expressed in E. coli. Substrate specificity studies on the gene led to the conclusion that it could accept 3- oxopropionyl-CoA as a substrate besides acetoacetyl-CoA (Ploux et al., supra). Additional genes include Hbdl (C-terminal domain) and Hbd2 (N-terminal domain) in Clostridium kluyveri (Hillmer and Gottschalk, Biochim. Biophys. Acta 3334: 12-23 (1974)) and HSDl 7B10 in Bos taurus (Wakil et al., J. Biol. Chem. 207:631-638 (1954). Data related to the sequences for each of these exemplary gene products can be found using the following GenBank accession numbers shown in Table 6.

Table 6

A number of similar enzymes have been found in other species of Clostridia and in Metallosphaera sedula (Berg et al., Science 318: 1782-1786 (2007) as shown in Table 7.

Table 7

hbd NP J49314.1 Clostridium acetobutylicum hbd AAM 14586.1 Clostridium beijerinckii

Msed _1423 YP 001191505 Metallosphaera sedula

Msed 0399 YP 001190500 Metallosphaera sedula

Msed 0389 YP 001190490 Metallosphaera sedula

Msed 1993 YP 001192057 Metallosphaera sedula

Alternatively, there exists several exemplary alcohol dehydrogenases that convert a ketone to a hydroxyl functional group. Two such enzymes from E. coli are encoded by malate dehydrogenase (mdh) and lactate dehydrogenase (idhA). In addition, lactate dehydrogenase from Ralstonia eutropha has been shown to demonstrate high activities on substrates of various chain lengths such as lactate, 2-oxobutyrate, 2-oxopentanoate and 2-oxoglutarate Steinbuchel et al., Eur. J. Biochem., 130:329-334 (1983)).

Transformations in Figure 1 also rely on the two-step reduction of acyl-CoA to the corresponding alcohol. For example, step L in the butanol pathway and step P in the isobutanol pathway rely on this transformation. Exemplary two-step oxidoreductases that convert an acyl- CoA to alcohol include those that transform substrates such as acetyl-CoA to ethanol (e.g., adhE irom E. coli (Kessler et al., FEBS. Lett., 281 :59-63 (1991)) and butyryl-CoA to butanol (e.g. adhE2 from C acetobutylicum (Fontaine et al., J. Bacteriol, 184:821-830 (2002)). In addition to reducing acetyl-CoA to ethanol, the enzyme encoded by adhE in Leuconostoc mesenteroides has been shown to oxidize the branched chain compound isobutyraldehyde to isobutyryl-CoA ( Kazahaya et al., J. Gen. Appl. Microbiol., 18:43-55 (2005)). Data related to the sequences for each of these exemplary gene products can be found using the following GenBank accession numbers shown in Table 8.

Table 8

Another exemplary enzyme can convert malonyl-CoA to 3 -HP. An NADPH-dependent enzyme with this activity has characterized in Chloroflexus aurantiacus where it participates in the 3- hydroxypropionate cycle (Hugler et al., J. Bacteriol, 184:2404-2410 (2002); Strauss et al., Eur. J. Biochem., 215:633-643 (1993)). This enzyme, with a mass of 300 kDa, is highly substrate- specific and shows little sequence similarity to other known oxidoreductases (Hugler et al., supra). Enzymes in other organisms including Roseiflexus castenholzii, Erythrobacter sp. NAPl and marine gamma proteobacterium HTCC2080 can be inferred by sequence similarity. Data related to the sequences for each of these exemplary gene products can be found using the following GenBank accession numbers shown in Table 9.

Table 9

Longer chain acyl-CoA molecules can be reduced by enzymes such as the jojoba (Simmondsia chinensis) FAR which encodes an alcohol- forming fatty acyl-CoA reductase. Its overexpression in E. coli resulted in FAR activity and the accumulation of fatty alcohol (Metz et al., Plant Physiology, 122:635-644 (2000)) (FAR, AAD38039.1, Simmondsia chinensis).

The pathways disclosed herein also involve oxidoreductase-type transformations that convert an acyl-CoA to an aldehyde. Specifically, Steps J and N catalyze the reduction of butytyl-CoA to butyraldehyde and isobutyryl-CoA to isobutyraldehyde respectively. Several acyl-CoA dehydrogenases are capable of reducing an acyl-CoA to its corresponding aldehyde. Exemplary genes that encode such enzymes include the Acinetobacter calcoaceticus acrl encoding a fatty acyl-CoA reductase (Reiser et al., Journal of Bacteriology , 179:2969-2975 (1997)), the Acinetobacter sp. M-I fatty acyl-CoA reductase (Ishige et al., Environ. Microbiol, 68:1192- 1195 (2002)), and a CoA- and NADP- dependent succinate semialdehyde dehydrogenase encoded by the sucD gene in Clostridium kluyveri (Sohling et al., J. Bacteriol. 178:871-880 (1996)). SucD of P. gingivalis is another succinate semialdehyde dehydrogenase (Takahashi et al., J. Bacteriol 182:4704-4710 (2000)). The enzyme acylating acetaldehyde dehydrogenase in Pseudomonas sp, encoded by bphG, is yet another enzyme demonstrated to oxidize and acylate acetaldehyde, propionaldehyde, butyraldehyde, isobutyraldehyde and formaldehyde (Powlowski et al., J. Bacteriol. 175:377-385 (1993). In addition to reducing acetyl-CoA to ethanol, the enzyme encoded by adhE in Leuconostoc mesenteroides has been shown to oxidize the branched chain compound isobutyraldehyde to isobutyryl-CoA (Kazahaya et al. J. Gen. Appl. Microbiol. 18:43-55 (1972); Koo et al., Bacteriol Lett. 27:505-510 (2005)). Data related to the sequences for each of these exemplary gene products can be found using the following GenBank accession numbers shown in Table 10.

Table 10

An additional enzyme type that converts an acyl-CoA to its corresponding aldehyde is malonyl- CoA reductase which transforms malonyl-CoA to malonic semialdehyde. Malonyl-CoA reductase is a key enzyme in autotrophic carbon fixation via the 3-hydroxypropionate cycle in thermoacidophilic archaeal bacteria (Berg et al., Science 318:1782-1786 (2007); Thauer Science 318: 1732-1733 (2007)). The enzyme utilizes NADPH as a cofactor and has been characterized in Metallosphaera and Sulfolobus spp (Alber et al., supra; Hugler et al., J. Bacteriol.184:2404- 2410 (2002)). The enzyme is encoded by Msed_0709 in Metallosphaera sedula (Alber et al., supra; Berg et al., supra). A gene encoding a malonyl-CoA reductase from Sulfolobus tokodaii was cloned and heterologously expressed in E. coli ((Alber et al., supra). This enzyme has also been shown to catalyze the conversion of methylmalonyl-CoA to its corresponding aldehyde. Although the aldehyde dehydrogenase functionality of these enzymes is similar to the bifunctional dehydrogenase from Chloroflexus aurantiacus, there is little sequence similarity. Both malonyl-CoA reductase enzymes have high sequence similarity to aspartate-semialdehyde dehydrogenase, an enzyme catalyzing the reduction and concurrent dephosphorylation of aspartyl-4-phosphate to aspartate semialdehyde. Additional genes can be found by sequence homology to proteins in other organisms including Sulfolobus solfataricus and Sulfolobus acidocaldarius and have been listed below. Yet another enzyme for CoA-acylating aldehyde dehydrogenase is the aid gene from Clostridium beijerinckii (Toth et al., Appl. Environ

Microbiol. 65:4973-4980 (1999)). This enzyme has been reported to reduce acetyl-CoA and butyryl-CoA to their corresponding aldehydes. This gene is very similar to eutE that encodes acetaldehyde dehydrogenase of Salmonella typhimurium and E. coli (Toth et al., supra). Data related to the sequences for each of these exemplary gene products can be found using the following GenBank accession numbers shown in Table 11.

Table 11

Referring to Figure 1 , step I refers to the conversion of crotonyl-CoA to butyryl-CoA by crotonyl-CoA reductase and step S refers to the conversion of methacryl-CoA to isobutyryl-CoA by methacrylyl-CoA reductase. Enoyl-CoA reductase enzymes are enzymes that can carry out either step. One exemplary enoyl-CoA reductase is the gene product oϊbcd from C. acetobutylicum (Boynton et al., J. Bacteriol. 178:3015-3024 (1996); Atsumi et al., Metab. Eng. (2007)), which naturally catalyzes the reduction of crotonyl-CoA to butyryl-CoA. Activity of this enzyme can be enhanced by expressing bed in conjunction with expression of the C. acetobutylicum etfAB genes, which encode an electron transfer flavoprotein. An additional enzyme for the enoyl-CoA reductase step is the mitochondrial enoyl-CoA reductase from E. gracilis (Hoffmeister et al., J. Biol. Chem. 280:4329-4338 (2005)). A construct derived from this sequence following the removal of its mitochondrial targeting leader sequence was cloned in E. coli resulting in an active enzyme (Hoffmeister et al., supra). This approach is well known to those skilled in the art of expressing eukarytotic genes, particularly those with leader sequences that can target the gene product to a specific intracellular compartment, in prokaryotic organisms. A close homolog of this gene, TDE0597, from the prokaryote Treponema denticola represents a third enoyl-CoA reductase which has been cloned and expressed in E. coli (Tucci et al., FEBS Lett, 581 :1561-1566 (2007)). Data related to the sequences for each of these exemplary gene products can be found using the following GenBank accession numbers shown in Table 12. Table 12

Isobutyryl-CoA dehydrogenase is another enzyme for step S of Figure 1, though it naturally catalyzes the oxidation of isobutyryl-CoA to methacrylyl-CoA. The crystal structure of the human isobutyryl-CoA dehydrogenase with and without the bound substrate has been determined (Battaile et al., J. Biol. Chem. 279: 16526-16534 (2004)). Additional isobutyryl- CoA dehydrogenases from Mus musculus and Rhodopseudomonas palustris can be inferred by sequence similarity. Data related to the sequences for each of these exemplary gene products can be found using the following GenBank accession numbers shown in Table 13.

Table 13

Yet further enzymes include 2-enoate reductases (EC 1.3.1.31) that are known to catalyze the NADH-dependent reduction of a wide variety of α, β-unsaturated carboxylic acids and aldehydes (Rohdich et al., J. Biol. Chem. 276:5779-5787 (2001)). 2-enoate reductase is encoded by enr in several species of Clostridia (Giesel, et al. Arch. Microbiol. 135:51-57 (1983)) including C tyrobutyricum, and C thermoaceticum (now called Moorella thermoaceticum) (Rohdich et al., supra). In the recently published genome sequence of C kluyveri, 9 coding sequences for enoate reductases have been reported, out of which one has been characterized (Seedorf et al, Proc. Natl. Acad. Sci. U.S.A. 105:2128-2133 (2008). The enr genes from both C. tyrobutyricum and C thermoaceticum have been cloned and sequenced and show 59% identity to each other. The former gene is also found to have approximately 75% similarity to the characterized gene in C kluyveri (Giesel et al., supra). It has been reported based on these sequence results that enr is very similar to the dienoyl CoA reductase in E. coli (fadH) (Rohdich et al., supra). The C thermoaceticum enr gene has also been expressed in an enzymatically active form in E. coli (Rohdich et al., supra). Data related to the sequences for each of these exemplary gene products can be found using the following GenBank accession numbers shown in Table 14.

Table 14

Step E of Figure 1 refers to the conversion of acetoacetyl-CoA to acetoacetate by acetyl- CoA:acetoacetate-CoA transferase or a similar transferase. The E. coli enzyme encoded by atoA (alpha subunit) and atoD (beta subunit) (Vanderwinkel et al., Biochem. Biophys. Res. Comm. 33:902-908 (1968)); Korolev et al., Acta Crystallagr. D Biol Crystallagr. 58:2116-2121 (2002)), also known as acetate-CoA transferase (EC 2.8.3.8), catalyzes this exact transformation and has additionally been shown to transfer the CoA moiety to acetate from a variety of branched and linear acyl-CoA substrates, including isobutyrate (Matthies et al., Appl. Environ. Microbiol. 58:1435-1439 (1992)), valerate (Vanderwinkel supra) and butanoate (Vanderwinkel supra). Similar enzymes exist in Corynebacterium glutamicum ATCC 13032 (Duncan et al., Appl. Environ. Miocrobiol. 68:5186-5190 (2002)), Clostridium acetobutylicum (90), and Clostridium saccharoperbutylacetonicum (112). Data related to the sequences for each of these exemplary gene products can be found using the following GenBank accession numbers shown in Table 15.

Table 15

Additional exemplary transferase transformations are catalyzed by the gene products oicatl, cat2, and cat3 of Clostridium kluyveri which have been shown to exhibit succinyl-CoA, 4- hydroxybutyryl-CoA, and butyryl-CoA acetyltransferase activity, respectively (Aberthart et al., J. Chem. Soc. 6: 1404-1406 (1979); Agnihotri et al. Med. Chem. 11 :9-20 (2003)). Data related to the sequences for each of these exemplary gene products can be found using the following GenBank accession numbers shown in Table 16.

Table 16

The glutaconate-CoA-transferase (EC 2.8.3.12) enzyme from anaerobic bacterium Acidaminococcus fermentans reacts with diacid glutaconyl-CoA and 3-butenoyl-CoA (Mack et al., FEBS Lett. 405:209-212 (1997)). The genes encoding this enzyme are gctA and gctB. This enzyme has reduced but detectable activity with other CoA derivatives including glutaryl-CoA, 2-hydroxyglutaryl-CoA, adipyl-CoA and acrylyl-CoA (Buckel et al., supra). The enzyme has been cloned and expressed in is. coli (Mack et al., Eur. J. Biochem. 226:41-51 (1994)). Data related to the sequences for each of these exemplary gene products can be found using the following GenBank accession numbers shown in Table 17.

Table 17

Additional enzymes capable of converting acetoacetyl-CoA to acetoacetate include succinyl- CoA:3-ketoacid CoA transferases which utilize succinate as the CoA acceptor. Exemplary succinyl-CoA:3:ketoacid-CoA transferases are present in Helicobacter pylori (Corthesy-Theulaz et al., J. Biol. Chem. 272:25659-25667 (1997)) and Bacillus subtilis (Stols et al., Protein. Expr. Purif 53:396-403 (2007)). Data related to the sequences for each of these exemplary gene products can be found using the following GenBank accession numbers shown in Table 18.

Table 18

HPAGl _0676 YP _627417 Helicobacter pylori

HPAGl _0677 YP _627418 Helicobacter pylori

ScoA NP _391778 Bacillus subtilis

ScoB NP _391777 Bacillus subtilis

Step F of Figure 1 refers to the conversion of acetoacetyl-CoA to acetoacetate by acetoacetyl- CoA hydrolase. Such activity has been detected in Rattus norvegicus (Patel et al., Biochem. J. Biochem. J. 176:951-958 (1978)), Bos taurus (Drummond et al., J. Biol. Chem. 235:318-325 (I960)), and Homo sapiens (Rous, Biochem. Biophys. Res. Commun. 69:74-78, (1976)), although the gene sequences encoding the corresponding enzymes are not known. Several eukaryotic acetyl-CoA hydrolases (EC 3.1.2.1) have broad substrate specificity and thus represent suitable enzymes for hydrolyzing acetoacetyl-CoA. For example, the enzyme from Rattus norvegicus brain, acotl2 (NP_570103.1) (Robinson, Jr. et al., Biochem. Biophys. Res. Comm. 71 :959-965 (1976)) can react with butyryl-CoA, hexanoyl-CoA and malonyl-CoA.

Additional hydrolase enzymes include 3-hydroxyisobutyryl-CoA hydrolase which has been described to efficiently catalyze the conversion of 3 -hydroxyisobutyryl-CoA to 3- hydroxyisobutyrate during valine degradation (Shimomura et al., J. Biol. Chem. 269:14248- 14253 (1994)). Genes encoding this enzyme include hibch of Rattus norvegicus (Shimomura supra; Shimomura et al, 2000) and Homo sapiens (Shimomura et al., Methods Enzymol. 324:229-240 (2000)). Genes identified by sequence homology include hibch of Saccharomyces cerevisiae and BCJ2292 of Bacillus cereus. Data related to the sequences for each of these exemplary gene products can be found using the following GenBank accession numbers shown in Table 19.

Table 19

Yet another hydrolase is the human dicarboxylic acid thioesterase, acot8, which exhibits activity on glutaryl-CoA, adipyl-CoA, suberyl-CoA, sebacyl-CoA, and dodecanedioyl-CoA (Westin et al., J. Biol. Chem. 280:38125-38132 (2005)) and the closest E. coli homolog, tesB, which can also hydrolyze a broad range of CoA thiolesters (Naggert et al., J. Biol. Chem. 266:11044-11050 (1991)). A similar enzyme has also been characterized in the rat liver (Deana, Biochem. Int. 26:767-773 (1992)). Data related to the sequences for each of these exemplary gene products can be found using the following GenBank accession numbers shown in Table 20.

Table 20

Other potential E. coli thiolester hydrolases include the gene products of tesA (Bonner et al., J. Biol. Chem. 247:3123-3133 (1972)), ybgC (Kuznetsova et al., FEMS Microbiol. Rev. 29:263- 279 (2005); Zhuang et al., FEBS Lett. 516:161-163 (2002)), paal (Song et al., J. Biol. Chem. 281 : 11028-11038 (2006)), and jM5 (Leduc et al., J. Bacteriol. 189: 7112-7126 (2007)). Data related to the sequences for each of these exemplary gene products can be found using the following GenBank accession numbers shown in Table 21.

Table 21

In Figure 1, step G, acetoacetate is decarboxylated to form acetone. This reaction can be catalyzed by acetoacetate decarboxylase (EC 4.1.1.4), an enzyme studied for its role in bacterial solventogenesis. Exemplary bacterial enzymes have been characterized from Clostridium acetobutylicum (Benner et al., J. Am. Chem. So. 103:993-994 (1981); HIghbarger et al., Biochemistry 35:41-46 (1996); Petersen et al., Appl. Environ. Microbiol. 56:3491-3498 (1990); Rozzel et al. J. Am. Chem. Soc.106:4937-4941 (1984)) and Clostridium beijerinckii (Ravagnani et al. MoI. Microbiol. 37:1172-1185 (2000)). Acetoacetate decarboxylase activity has also been demonstrated in Pseudomonas putida and Bacillus polymyxa but genes are not associated with this activity to date (Matiasek et al., Curr. Microbiol. 42: 276-281 (2001)). Bacterial genes in other organisms such as Clostridium botulinum and Bacillus amyloliquefaciens FZB42 can be identified by sequence homology. In humans and other mammals, acetoacetate decarboxylase catalyzes the final step of the ketone-body pathway (Kalapos, Biochim. Biophys. Acta 1621 : 122- 139 (2003)), but genes associated with this activity have not been identified to date. Data related to the sequences for each of these exemplary gene products can be found using the following GenBank accession numbers shown in Table 22.

Table 22

Other exemplary decarboxylase enzymes include pyruvate decarboxylase (EC 4.1.1.1) and benzoylformate decarboxylase (EC 4.1.1.7). Pyruvate decarboxylase (PDC), also termed keto- acid decarboxylase, is a key enzyme in alcoholic fermentation, catalyzing the decarboxylation of pyruvate to acetaldehyde. The enzyme from Saccharomyces cerevisiae has a broad substrate range for aliphatic 2-keto acids including 2-ketobutyrate, 2-ketovalerate, 3-hydroxypyruvate and 2-phenylpyruvate (Li et al., Biochemistry 38:10004-10012 (1999)). This enzyme has been extensively studied, engineered for altered activity, and functionally expressed in E. coli (Killenberg-Jabs et al., Eur. J. Biochem. 268:1698-1704 (2001); Li et al., supra; ter Schure et al., Appl. Environ. Micriobol. 64:1303-1307 (1998)). The PDC from Zymomonas mobilus, encoded by pdc, also has a broad substrate range and has been a subject of directed engineering studies to alter the affinity for different substrates (Siegert et al., Protein Eng. Des. SeL, 18:345- 357 (2005)). The crystal structure of this enzyme is available (Killenberg-Jabs supra). Other well-characterized PDC enzymes include the enzymes from Acetobacter pasteurians (Chandra et al., Arch. Microbiol. 176:443-451 (2001)) and Kluyveromyces lactis (Krieger et al., Eur. J. Biochem.269:3256-3263 (2002)). Data related to the sequences for each of these exemplary gene products can be found using the following GenBank accession numbers shown in Table 23.

Table 23

Like PDC, benzoylformate decarboxylase (EC 4.1.1.7) has a broad substrate range and has been the target of enzyme engineering studies. The enzyme from Pseudomonas putida has been extensively studied and crystal structures of this enzyme are available (Polovnikova et al., Biochemistry 42: 1820-1830 (2003); Hasson et al., Biochemistry 37:9918-9930 (1998)). Site- directed mutagenesis of two residues in the active site of the Pseudomonas putida enzyme altered the affinity (Km) of naturally and non-naturally occuring substrates (Siegert et al., Protein Eng. Des. SeI, 18:345-357 (2005)). The properties of this enzyme have been further modified by directed engineering (Lingen et al., Chembiochem 4:721-726 (2003); Lingen et al., Protein Eng. 15:585-593 (2002)). The enzyme from Pseudomonas aeruginosa, encoded by mdlC, has also been characterized experimentally (Barrowman et al., FEMS Micriobiol. Letters 34:57-60 (1986)). Additional genes from Pseudomonas stutzeri, Pseudomonas fluorescens and other organisms can be inferred by sequence homology or identified using a growth selection system developed in Pseudomonas putida (Henning et al., Appl. Environ. Micribiol. 72:7510- 7517 (2006)). Data related to the sequences for each of these exemplary gene products can be found using the following GenBank accession numbers shown in Table 24.

Table 24

A third enzyme capable of decarboxylating 2-oxoacids is alpha-ketoglutarate decarboxylase (KGD). The substrate range of this class of enzymes has not been studied to date. The KDC from Mycobacterium tuberculosis (Tian et al., Proc. Natl. Acad. Sci. U.S.A. 102:10670-10675 (2005)) has been cloned and has been functionally expressed in E. coli at Genomatica. KDC enzyme activity has been detected in several species oiRhizobia including Bradyrhizobium japonicum and Mesorhizobium loti (Green et al., J. Bacteriol. 182:2838-2844 (2000)). Although the KDC-encoding gene(s) have not been isolated in these organisms, the genome sequences are available and several genes in each genome are annotated as putative KDCs. A KDC from Euglena gracilis has also been characterized but the gene associated with this activity has not been identified to date (Shigeoka et al., Arch. Biochem. Biophys. 288:22-28 (1991)). The first twenty amino acids starting from the w-terminus were sequenced

MTYKAPVKDVKFLLDKVFKV (Shigeoka et al., supra). The gene can be identified by testing genes containing this w-terminal sequence for KDC activity. Data related to the sequences for each of these exemplary gene products can be found using the following GenBank accession numbers shown in Table 25.

Table 25

Hydration of crotonyl-CoA to form 3-hydroxybutyryl-CoA (step B, Figure 1) is catalyzed by a crotonase (EC 4.2.1.55). These enzymes are part of the pathways for w-butanol formation in some organisms, particularly Clostridial species, and also comprise one step of the 3- hydroxypropionate/4-hydroxybutyrate cycle in thermoacidophilic Archaea of the genera Sulfolobus, Acidianus, and Metallosphaera. Exemplary genes encoding crotonase enzymes can be found in C acetobutylicum (Boynton et al., Journal of Bacteriology 178:3015-3024 (1996)), C kluyveri (Hillmer et al., FEBS Lett. 21 :351-354 (1972)), and Metallosphaera sedula (Berg et al., Archaea. Science. 318:1782-1786 (2007)) though the sequence of the latter gene is not known. Enoyl-CoA hydratases, which are involved in fatty acid beta-oxidation and/or the metabolism of various amino acids, can also catalyze the hydration of crotonyl-CoA to form 3- hydroxybutyryl-CoA (Agnihotri et al., Med. Chem., 11 :9-20 (2003); Conrad et al., J Bacteriol. 118: 103-111 (1974)). The enoyl-CoA hydratases, phaA and phaB, of P. putida have been indicated to carry out the hydroxylation of double bonds during phenylacetate catabolism (Olivera et al., Proc. Natl. Acad. Sci USA 95:6419-6424 (1998)). ThepaaA andpaaB from P. fluorescens catalyze analogous transformations (Olivera et al., supra). Lastly, a number of Escherichia coli genes have been shown to demonstrate enoyl-CoA hydratase functionality including maoC (Park et al., J Bacteriol. 185:5391-5397 (2003)), paaF (Ismail et al., Eur. J Biochem. 270:3047-3054 (2003); Park et al., Appl. Biochem. Biotechnol 113-116:335-346 (2004); Park et al., Biotechnol Bioeng 86:681-686 (2004)) andpaaG (Ismail et al., sw/?rø;Park et al., supra;Vark et al., supra). Data related to the sequences for each of these exemplary gene products can be found using the following GenBank accession numbers shown in Table 26.

Table 26

Alternatively, the E. coli gene products oifadA and fadB encode a multienzyme complex involved in fatty acid oxidation that exhibits enoyl-CoA hydratase activity (Haller et al., Biochemistry, 39:4622-4629 (2000), Martinez-Carrion et al., J. Biol. Chem. 240:3538-3546 (1965) and Matties et al., Appl. Environ. Microbiol., 58:1435-1439 (1992)). Knocking out a negative regulator encoded by fadR can be utilized to activate the fadB gene product (Jeng et al. Biochem. 13:2898-2903 (1974)). The fadl and fadJ genes encode similar functions and are naturally expressed under anaerobic conditions (Atsumi et al., Nature 451 :86-89 (2008)). Data related to the sequences for each of these exemplary gene products can be found using the following GenBank accession numbers shown in Table 27.

Table 27

The reversible conversion of 4-hydroxybutyryl-CoA to crotonyl-CoA (step A, Figure 1) is catalyzed by the bifunctional enzyme 4-hydroxybutyryl-CoA dehydratase/vinylacetyl-CoA Δ- isomerase. This enzyme first dehydrates 4-hydroxybutyryl-CoA to vinylacetyl-CoA, which subsequently rearranges to form crotonoyl-CoA. The enzymes from Clostridium kluyveri and C. aminobutyrium have been purified, characterized, and sequenced at the w-terminal domain (Scherf et al., Eur. J. Biochem. 215:421-429 (1993); Scherf et al., Arch. Microbiol 161 :239-245 (1994)). The abfD genes from C. aminobutyrium and C. kluyveri match exactly with these n- terminal amino acid sequences, and have been indicated to encode the 4-hydroxybutyrul-CoA dehydratases/vinylacetyl-CoA Δ-isomerase activities. In addition, the abfD gene from Porphyromonas gingivalis ATCC 33277 is identified through homology from genome projects. Data related to the sequences for each of these exemplary gene products can be found using the following GenBank accession numbers shown in Table 27.

Table 27

Dehydration of 3-hydroxyisobutyryl-CoA to methacrylyl-CoA (step R) can be accomplished by a reversible 3-hydroxyacyl-CoA dehydratase such as crotonase (also called 3-hydroxybutyryl- CoA dehydratase, EC 4.2.1.55) or enoyl-CoA hydratase (also called 3-hydroxyacyl-CoA dehydratase, EC 4.2.1.17). These enzymes are generally reversible (Moskowitz et al., Biochemistry 8:2748-2755 (1969); Durre et al., FEMS Microbiol Rev 17:251-262 (1995)). Exemplary enzymes are listed above. 3-hydroxyisobutyryl-CoA is not a natural substrate of these enzymes, but it is similar in structure to the native substrate, 3 -hydroxybutyryl-CoA.

Referring to Figure 1 , step M is carried out by isobutyryl-CoA mutase (ICM), a cobalamin- dependent methylmutase that reversibly rearranges the carbon backbone of butyryl-CoA into isobutyryl-CoA (Ratnatilleke et al., J Biol Chem 274:31679-31685 (1999)). Such an enzyme is also suitable for catalyzing the conversion of 4-hydroxybutyryl-CoA to 3-hydroxyisobutyryl- CoA, described by step Q of Figure 1. Genes encoding a heterodimeric ICM include icm and icmB of Streptomyces cinnamonensis (Ratnatilleke et al., supra; Vrijbloed et al., J Bacteriol 181 :5600-5605 (1999); Zerbe-Burkhardt et al., J Biol Chem 273:6508-6517 (1998)). Homologous genes in Streptomyces avermitilis MA-4680 likely catalyze the same or similar transformations. A novel ICM-like enzyme in β-Proteobacterium L 108 with nearly 100% identity with the corresponding peptide sequences from Methylibium petroleiphilum PMl was shown to convert 2-hydroxyisobutyryl-CoA to 3-hydroxybutyrate (Rohwerder et al., Appl Environ Microbiol 72:4128-4135 (2006)). This study also indicates that the replacement of the Phe residue in typical ICM enzymes (e.g., (Ratnatilleke et al., supra) with He at position 80 can be one of the mutations responsible for the activity on 2-hydroxyisobutyryl-CoA. This implies that if native ICM or ICM-like enzymes are unable to naturally catalyze the conversion of 4- hydroxybutyryl-CoA to 3-hydroxyisobutyryl-CoA, they could be engineered to do so. Data related to the sequences for each of these exemplary gene products can be found using the following GenBank accession numbers shown in Table 28.

Table 28

Methylmalonyl-CoA mutase (MCM) enzymes represent an additional suitable class of enzymes for catalyzing the transformations described by steps P or T of Figure 3, provided they naturally exhibit or can be engineered to exhibit activity on butyryl-CoA or 4-hydroxybutyryl-CoA, respectively. MCM naturally catalyzes the conversion of succinyl-CoA into methylmalonyl- CoA. In E. coli, the reversible adenosylcobalamin-dependant mutase participates in a three-step pathway leading to the conversion of succinate to propionate (Haller et al., Biochemistry. 39:4622-4629 (2000)). MCM is encoded by genes scpA in Escherichia coli (Haller et al., supra; Bobik et al., Anal Bioanal Chem 375:344-349 (2003)) and mutA in Homo sapiens (Padovani et al., Biochemistry 45:9300-9306 (2006)). In several other organisms MCM contains alpha and beta subunits and is encoded by two genes. Exemplary genes encoding the two-subunit protein are Propionibacterium fredenreichii sp. shermani mutA and mutB and Methylobacterium extorquens mcmA and mcmB (Korotkova et al., J Biol Chem. 279:13652-13658 (2004)). Data related to the sequences for each of these exemplary gene products can be found using the following GenBank accession numbers shown in Table 29.

Table 29

These sequences can be used to identify homologue proteins in GenBank or other databases through sequence similarity searches (for example, BLASTp). The resulting homologue proteins and their corresponding gene sequences provide additional exogenous DNA sequences for transformation into E. coli or other suitable host microorganisms to generate production hosts. Additional genes include the following, which were identified based on high homology to the E. coli spcA gene product. Data related to the sequences for each of these exemplary gene products can be found using the following GenBank accession numbers shown in Table 30.

Table 30

There exists evidence that genes adjacent to the methylmalonyl-CoA mutase catalytic genes contribute to maximum activity. For example, it has been demonstrated that the meaB gene from M. extorquens forms a complex with methylmalonyl-CoA mutase, stimulates in vitro mutase activity, and possibly protects it from irreversible inactivation (Korotkova et al., supra). The M. extorquens meaB gene product is highly similar to the product of the E. coli argK gene (BLASTp: 45% identity, e-value: 4e-67), which is adjacent to scpA on the chromosome. No sequence for a meaB homolog in P. freudenreichii is catalogued in GenBank. However, the Propionibacterium acnes KPA171202 gene product, YP_055310.1, is 51% identical to the M. extorquens meaB protein and its gene is also adjacent to the methylmalonyl-CoA mutase gene on the chromosome. Data related to the sequences for each of these exemplary gene products can be found using the following GenBank accession numbers shown in Table 31.

Table 31

E. coli can synthesize adenosylcobalamin, a necessary cofactor for this reaction, when supplied with the intermediates cobinamide or cobalamin (Lawrence et al., J Bacteriol 177:6371-6380 (1995); Lawrence et al., Genetics 142:11-24 (1996)). Alternatively, the ability to synthesize cobalamins de novo has been conferred upon E. coli following the expression of heterologous genes (Raux et al., J Bacteriol 178:753-767 (1996)).

Step D in Figure 1 refers to an acid-thiol ligase which catalyzes the conversion of acetoacetyl- CoA to acetoacetate. An exemplary acid-thiol ligase is the enzyme encoded by sucCD of is. coli which catalyzes the formation of succinyl-CoA from succinate with the concomitant consumption of one ATP, a reaction which is reversible in vivo (Buck et al., Biochemistry 24:6245-6252 (1985)). Additional enzymes are acetoacetyl-CoA synthetases from Mus musculus (Hasegawa et al., Biochim Biophys Acta 1779:414-419 (2008)) and Homo sapiens (Ohgami et al., Biochem Pharmacol 65:989-994 (2003)) which naturally catalyze the ATP-dependant conversion of acetoacetate into acetoacetyl-CoA. Such enzymes can convert acetoacetyl-CoA to acetoacetate should they exhibit acetoacetyl-CoA hydrolase activity. Data related to the sequences for each of these exemplary gene products can be found using the following GenBank accession numbers shown in Table 32. Table 32

sucC NP_ 415256 .1 Escherichia coli sucD AAC73823 1 Escherichia coli

AACS NP_ _084486 .1 Mus musculus

AACS NP_ 076417 .2 Homo sapiens

ADP-forming acetyl-CoA synthetase (ACD, EC 6.2.1.13) is another enzyme that couples the conversion of acyl-CoA esters to their corresponding acids with the concurrent synthesis of ATP. Although this enzyme has not been shown to react with acetoacetyl-CoA as a substrate, several enzymes with broad substrate specificities have been described in the literature. ACD I from Archaeoglobus fulgidus, encoded by AF 1211, was shown to operate on a variety of linear and branched-chain substrates including acetyl-CoA, propionyl-CoA, butyryl-CoA, acetate, propionate, butyrate, isobutyryate, isovalerate, succinate, fumarate, phenylacetate, indoleacetate (Musfeldt et al., JBacteriol 184:636-644 (2002)). The enzyme from Haloarcula marismortui (annotated as a succinyl-CoA synthetase) accepts propionate, butyrate, and branched-chain acids (isovalerate and isobutyrate) as substrates, and was shown to operate in the forward and reverse directions (Brasen et al., Arch Microbiol 182:277-287 (2004)). The ACD encoded by PAE3250 from hyperthermophilic crenarchaeon Pyrobaculum aerophilum showed the broadest substrate range of all characterized ACDs, reacting with acetyl-CoA, isobutyryl-CoA (preferred substrate) and phenylacetyl-CoA (Brasen et al., supra). The enzymes from A. fulgidus, H. marismortui and P. aerophilum have all been cloned, functionally expressed, and characterized in E. coli (Musfeldt et al., swprø;Brasen et al., supra). The net transformation depicted by step D of Figure 1 can also be carried out by two enzymes such as acetate kinase and phosptransacetylase or butyrate kinase and phosphotransbutyrylase. Data related to the sequences for each of these exemplary gene products can be found using the following GenBank accession numbers shown in Table 33.

Table 33

The non-naturally occurring microbial organism capable of producing isopropanol from A- hydroxybutyryl-CoA can include a 4-hydroxybutyryl-CoA dehydratase encoded by one or more genes selected from the group consisting oifumA,fumB,fumC,fumH,fuml, MmcB, MmcC, hmd, BACCAP '_02294, ANACOLJJ2527, NtherDRAFT_2368, dmclA, dmclB, crt, crtl,paaA, paaB,phaA,phaB, maoC, paaF, paaG, abfl), and Msed_l 220. In particular embodiments the A- hydroxybutyryl-CoA dehydratase is encoded by abfl).

The non-naturally occurring microbial organism capable of producing isopropanol from A- hydroxybutyryl-CoA can include a crotonase encoded by one or more genes selected from the group consisting oifumA,fumB,fumC,fumH,fuml, MmcB, MmcC, hmd, BACCAP _ 02294, ANACOL_02527, NtherDRAFT_2368, dmdA, dmdB, crt, crtl, paaA,paaB,phaA,phaB, maoC, paaF,paaG, abfD, and Msed_1220. In particular embodiments, the crotonase is encoded by one or more genes selected from the group consisting of crt, crtl,paaA, paaB,phaA,phaB, maoC, paaF, and paaG.

The non-naturally occurring microbial organism capable of producing isopropanol from A- hydroxybutyryl-CoA can include a 3-hydroxybutyryl-CoA dehydrogenase encoded by one or more genes selected from the group consisting oithrA, akthr2, homo, homl, hom2,fadB,fadJ, Hbd2, Hbdl, hbd, HSD17B10, phbB,phaB, Msed_1423, Msed_0399, Msed_0389, Msed_1993, adh, adhA, adh-A, mdh, idhA, Idh, and bdh. In particular embodiments, the 3-hydroxybutyryl- CoA dehydrogenase is encoded by one or more genes selected from the group consisting oϊhbd, Hbd2, Hbdl, Msed_1423, Msed_0399, Msed_0389, Msed_1993,fadB, and fadJ.

The non-naturally occurring microbial organism capable of producing isopropanol from A- hydroxybutyryl-CoA can include an acetoacetyl-CoA synthetase encoded by one or more genes selected from the group consisting of sucC, sucD, AACS, AF1211, scs, and PAE3250. In particular embodiments, the acetoacetyl-CoA synthetase is encoded by one or more genes selected from the group consisting of sucC, sucD, AACS, and AF1211.

The non-naturally occurring microbial organism capable of producing isopropanol from A- hydroxybutyryl-CoA can include an acetyl-CoA:acetoacetate-CoA transferase encoded by one or more genes selected from the group consisting of atoA, atoD, actA, cgO592, ctfA, ctfB, catl, cat2, cat3, gctA, gctB, HPAGl _0676, HPAGl _0677, ScoA, and ScoB. In particular embodiments, the acetyl-CoA:acetoacetate-CoA transferase is encoded by one or more genes selected from the group consisting oiatoA, atoD, actA, cgO592, ctfA, ctfB, HPAGl _0676, HPAGl _0677, ScoA, and ScoB.

The non-naturally occurring microbial organism capable of producing isopropanol from A- hydroxybutyryl-CoA can include an acetoacetyl-CoA hydrolase encoded by one or more genes selected from the group consisting oϊacotl2, hibch, BCJ2292, tesB, acot8, tesA, ybgC, paal, and ybdB. In particular embodiments, the acetoacetyl-CoA hydrolase is encoded by one or more genes selected from the group consisting oϊacotl2, hibch, and tesA.

The non-naturally occurring microbial organism capable of producing isopropanol from A- hydroxybutyryl-CoA can include an acetoacetate decarboxylase is encoded by one or more genes selected from the group consisting oipdc,pdcl, mdlC, dpgB, UvB-I, kgd, kdcA, lysA, panD, dmpH, dmpE, xylll, xyllll, Reut_B5691, Reut_B5692, CAD,padl,pofK, (pad), padC, and pad. The non-naturally occurring microbial organism capable of producing isopropanol from A- hydroxybutyryl-CoA can also include an acetoacetate decarboxylase encoded by one or more genes selected from the group consisting of Adc, cbei_3835, CLL_A2135, RBAM_030030.

The non-naturally occurring microbial organism capable of producing isopropanol from A- hydroxybutyryl-CoA can include an acetone reductase encoded by one or more genes selected from the group consisting oithrA, akthr2, homo, homl, hom2,fadB,fadJ, Hbd2, Hbdl, hbd, HSD17B10, phbB,phaB, Msed_1423, Msed_0399, Msed_0389, Msed_1993, adh, adhA, adh-A, mdh, idhA, Idh, and bdh. In particular embodiments, the acetone reductase is encoded by one or more genes selected from the group consisting oϊadh, adhA, and adh-A.

The non-naturally occurring microbial organism capable of producing w-butanol from A- hydroxybutyryl-CoA can include a 4-hydroxybutyryl-CoA dehydratase encoded by one or more genes selected from the group consisting oifumA,fumB,fumC,fumH,fuml, MmcB, MmcC, hmd, BACCAP '_02294, ANACOLJJ2527, NtherDRAFT_2368, dmdA, dmdB, crt, crtl,paaA, paaB,phaA,phaB, maoC, paaF, paaG, abfl), and Msed_l 220. In particular embodiments the A- hydroxybutyryl-CoA dehydratase is encoded by abfl).

The non-naturally occurring microbial organism capable of producing w-butanol from A- hydroxybutyryl-CoA can include a crotonoyl-CoA reductase encoded by one or more genes selected from the group consisting of bed, etfA, etβ, Ter, TDE0597, IBD, RPA3448, FadH, and enr. In particular embodiments, the crotonoyl-CoA reductase is encoded by one or more genes selected from the group consisting oϊbcd, etfA, etfB, Ter, and TDE0597.

The non-naturally occurring microbial organism capable of producing w-butanol from A- hydroxybutyryl-CoA can include a butyryl-CoA reductase (aldehyde forming) encoded by one or more genes selected from the group consisting oiacrl, sucD, bphG, adhE, Msed_0709, mcr, asd-2, Saci_2370, Aid, and eutE. In particular embodiments, the butyryl-CoA reductase (aldehyde forming) is encoded by one or more genes selected from the group consisting of sucD, bphG, Msed_0709, mcr, and Aid.

The non-naturally occurring microbial organism capable of producing w-butanol from A- hydroxybutyryl-CoA can include a butyraldehyde reductase encoded by one or more genes selected from the group consisting oialrA, ADH2, yqhD, bdh I, bdh II, adhA, 4hbd, adhl, P84067, mmsb, dhat, and 3hidh. In particular embodiments, the butyraldehyde reductase is encoded by one or more genes selected from the group consisting oialrA, ADH2, yqhD, bdh I, bdh II, 4hbd, adhl, and mmsb.

The non-naturally occurring microbial organism capable of producing w-butanol from A- hydroxybutyryl-CoA can include a butyryl-CoA reductase (alcohol forming) encoded by one or more genes selected from the group consisting oiadhE, adhE2, mcr, Rcas_2929, NAPl_02720, MGP2080_00535, and FAR. In particular embodiments, the butyryl-CoA reductase (alcohol forming) is encoded by one or more genes selected from the group consisting oiadhE2, mcr, and FAR.

The non-naturally occurring microbial organism capable of producing isobutanol from A- hydroxybutyryl-CoA can include a 4-hydroxybutyryl-CoA dehydratase encoded by one or more genes selected from the group consisting oifumA,fumB,fumC,fumH,fuml, MmcB, MmcC, hmd, BACCAF '_02294, ANACOL_02527, NtherDRAFT_2368, dmdA, dmdB, crt, crtl,paaA, paaB,phaA,phaB, maoC, paaF , paaG, abfD, and Msed_l 220. In particular embodiments, the 4-hydroxybutyryl-CoA dehydratase is encoded by crt, crtl,paaA, paaB, phaA, phaB, maoC, paaF, and paa G.

The non-naturally occurring microbial organism capable of producing isobutanol from A- hydroxybutyryl-CoA can include a crotonoyl-CoA reductase encoded by one or more genes selected from the group consisting of bed, etfA, etβ, Ter, TDE0597, IBD, RP A3448, FadH, and enr. In particular embodiments, the crotonoyl-CoA reductase is encoded by one or more genes selected from the group consisting of bed, etfA, etfB, Ter, and TDE0597.

The non-naturally occurring microbial organism capable of producing isobutanol from A- hydroxybutyryl-CoA can include a isobutyryl-CoA mutase encoded by one or more genes selected from the group consisting oi icm, icmB, icmA, Mpe_B0538, Mpe_B0541, scpA, mutA, mutB, mcmA, mcmB, sbm, SARI_04585, YfreAJ)! 000861, argK, PPA0597, and meaB. In particular embodiments, the isobutyryl-CoA mutase is encoded by one or more genes selected from the group consisting oiicmB, icmA, Mpe_B0538, and Mpe_B0541.

The non-naturally occurring microbial organism capable of producing isobutanol from A- hydroxybutyryl-CoA can include a 4-hydroxybutyryl-CoA mutase encoded by one or more genes selected from the group consisting oiicm, icmB, icmA, Mpe_B0538, Mpe_B0541, scpA, mutA, mutB, mcmA, mcmB, sbm, SARI_04585, YfreAJ) 1000861, argK, PPA0597, and meciB. In particular embodiments the 4-hydroxybutyryl-CoA mutase is encoded by one or more genes selected from the group consisting oi icmB, icmA, Mpe_B0538, and Mpe_B0541.

The non-naturally occurring microbial organism capable of producing isobutanol from A- hydroxybutyryl-CoA can include a 3-hydroxyisobutyryl-CoA dehydratase encoded by one or more genes selected from the group consisting oifumA,fumB,fumC,fumH,fuml, MmcB, MmcC, hmd, BACCAP _02294, ANACOL_02527, NtherDRAFT_2368, dmdA, dmdB, crt, crtl, paaA, paaB, phaA, phaB, maoC, paaF, paaG, abfD, and Msed_1220. In particular embodiments, the 3-hydroxyisobutyryl-CoA dehydratase is encoded by one or more genes selected from the group consisting of crt, crtl,paaA, paaB, phaA, phaB, maoC, paaF, and paaG.

The non-naturally occurring microbial organism capable of producing isobutanol from A- hydroxybutyryl-CoA can include a methacrylyl-CoA reductase encoded by one or more genes selected from the group consisting of bed, etfA, etβ, Ter, TDE0597, IBD, RP A3448, FadH, and enr. In particular embodiments, the methacrylyl-CoA reductase is encoded by one or more genes selected from the group consisting oϊbcd, etfA, etβ, Ter, and TDE0597.

The non-naturally occurring microbial organism capable of producing isobutanol from A- hydroxybutyryl-CoA can include a isobutyryl-CoA reductase (aldehyde forming) encoded by one or more genes selected from the group consisting oiacrl, sucD, bphG, adhE, Msed_0709, mcr, asd-2, Saci_2370, Aid, and eutE. In particular embodiments, the isobutyryl-CoA reductase (aldehyde forming) is encoded by one or more genes selected from the group consisting of sucD, bphG, Msed_0709, mcr, and Aid.

The non-naturally occurring microbial organism capable of producing isobutanol from A- hydroxybutyryl-CoA can include a isobutyraldehyde reductase encoded by one or more genes selected from the group consisting oialrA, ADH2, yqhD, bdh I, bdh II, adhA, 4hbd, adhl,

P84067, mmsb, dhat, and 3hidh. In particular embodiments the isobutyraldehyde reductase is encoded by one or more genes selected from the group consisting oϊalrA, ADH2, yqhD, bdh I, bdh II, 4hbd, adhl, P84067, and mmsb.

The non-naturally occurring microbial organism capable of producing isobutanol from A- hydroxybutyryl-CoA can include an isobutyryl-CoA reductase (alcohol forming) encoded by one or more genes selected from the group consisting oiadhE, adhE2, mcr, Rcas_2929, NAPl_02720, MGP2080_00535, and FAR. In particular embodiments, the isobutyryl-CoA reductase (alcohol forming) is encoded by one or more genes selected from the group consisting oiadhE2, mcr, and FAR.

The non-naturally occurring microbial organisms of the invention can be produced by introducing expressible nucleic acids encoding one or more of the enzymes or proteins participating in one or more isopropanol, M-butanol, or isobutanol biosynthetic pathways. Depending on the host microbial organism chosen for biosynthesis, nucleic acids for some or all of a particular isopropanol, M-butanol, or isobutanol biosynthetic pathway can be expressed. For example, if a chosen host is deficient in one or more enzymes or proteins for a desired biosynthetic pathway, then expressible nucleic acids for the deficient enzyme(s) or protein(s) are introduced into the host for subsequent exogenous expression. Alternatively, if the chosen host exhibits endogenous expression of some pathway genes, but is deficient in others, then an encoding nucleic acid is needed for the deficient enzyme(s) or protein(s) to achieve isopropanol, M-butanol, or isobutanol biosynthesis. Thus, a non-naturally occurring microbial organism of the invention can be produced by introducing exogenous enzyme or protein activities to obtain a desired biosynthetic pathway or a desired biosynthetic pathway can be obtained by introducing one or more exogenous enzyme or protein activities that, together with one or more endogenous enzymes or proteins, produces a desired product such as isopropanol, M-butanol, or isobutanol.

Depending on the isopropanol, M-butanol, or isobutanol biosynthetic pathway constituents of a selected host microbial organism, the non-naturally occurring microbial organisms of the invention will include at least one exogenously expressed isopropanol, M-butanol, or isobutanol pathway-encoding nucleic acid and up to all encoding nucleic acids for one or more isopropanol, M-butanol, or isobutanol biosynthetic pathways. For example, isopropanol, M-butanol, or isobutanol biosynthesis can be established in a host deficient in a pathway enzyme or protein through exogenous expression of the corresponding encoding nucleic acid. In a host deficient in all enzymes or proteins of an isopropanol, M-butanol, or isobutanol pathway, exogenous expression of all enzyme or proteins in the pathway can be included, although it is understood that all enzymes or proteins of a pathway can be expressed even if the host contains at least one of the pathway enzymes or proteins. For example, exogenous expression of all enzymes or proteins in a pathway for production of isopropanol, «-butanol, or isobutanol can be included.

Given the teachings and guidance provided herein, those skilled in the art will understand that the number of encoding nucleic acids to introduce in an expressible form will, at least, parallel the isopropanol, w-butanol, or isobutanol pathway deficiencies of the selected host microbial organism. Therefore, a non-naturally occurring microbial organism of the invention can have one, two, three, four, five, up to all nucleic acids encoding the enzymes or proteins constituting an isopropanol, w-butanol, or isobutanol biosynthetic pathway disclosed herein. In some embodiments, the non-naturally occurring microbial organisms also can include other genetic modifications that facilitate or optimize isopropanol, w-butanol, or isobutanol biosynthesis or that confer other useful functions onto the host microbial organism. One such other functionality can include, for example, augmentation of the synthesis of one or more of the isopropanol, «-butanol, or isobutanol pathway precursors such as acetyl-CoA.

Generally, a host microbial organism is selected such that it produces the precursor of an isopropanol, w-butanol, or isobutanol pathway, either as a naturally produced molecule or as an engineered product that either provides de novo production of a desired precursor or increased production of a precursor naturally produced by the host microbial organism. For example, acetyl-CoA is produced naturally in a host organism such as E. coli. A host organism can be engineered to increase production of a precursor, as disclosed herein. In addition, a microbial organism that has been engineered to produce a desired precursor can be used as a host organism and further engineered to express enzymes or proteins of an isopropanol, w-butanol, or isobutanol pathway.

In some embodiments, a non-naturally occurring microbial organism of the invention is generated from a host that contains the enzymatic capability to synthesize isopropanol, n- butanol, or isobutanol. In this specific embodiment it can be useful to increase the synthesis or accumulation of an isopropanol, «-butanol, or isobutanol pathway product to, for example, drive isopropanol, w-butanol, or isobutanol pathway reactions toward isopropanol, w-butanol, or isobutanol production. Increased synthesis or accumulation can be accomplished by, for example, overexpression of nucleic acids encoding one or more of the above-described isopropanol, w-butanol, or isobutanol pathway enzymes or proteins. Over expression the enzyme or enzymes and/or protein or proteins of the isopropanol, w-butanol, or isobutanol pathway can occur, for example, through exogenous expression of the endogenous gene or genes, or through exogenous expression of the heterologous gene or genes. Therefore, naturally occurring organisms can be readily generated to be non-naturally occurring microbial organisms of the invention, for example, producing isopropanol, «-butanol, or isobutanol, through overexpression of one, two, three, four, five, that is, up to all nucleic acids encoding isopropanol, w-butanol, or isobutanol biosynthetic pathway enzymes or proteins. In addition, a non-naturally occurring organism can be generated by mutagenesis of an endogenous gene that results in an increase in activity of an enzyme in the isopropanol, «-butanol, or isobutanol biosynthetic pathway.

In particularly useful embodiments, exogenous expression of the encoding nucleic acids is employed. Exogenous expression confers the ability to custom tailor the expression and/or regulatory elements to the host and application to achieve a desired expression level that is controlled by the user. However, endogenous expression also can be utilized in other embodiments such as by removing a negative regulatory effector or induction of the gene's promoter when linked to an inducible promoter or other regulatory element. Thus, an endogenous gene having a naturally occurring inducible promoter can be up-regulated by providing the appropriate inducing agent, or the regulatory region of an endogenous gene can be engineered to incorporate an inducible regulatory element, thereby allowing the regulation of increased expression of an endogenous gene at a desired time. Similarly, an inducible promoter can be included as a regulatory element for an exogenous gene introduced into a non-naturally occurring microbial organism.

It is understood that, in methods of the invention, any of the one or more exogenous nucleic acids can be introduced into a microbial organism to produce a non-naturally occurring microbial organism of the invention. The nucleic acids can be introduced so as to confer, for example, isopropanol, «-butanol, or isobutanolbiosynthetic pathway onto the microbial organism. Alternatively, encoding nucleic acids can be introduced to produce an intermediate microbial organism having the biosynthetic capability to catalyze some of the required reactions to confer isopropanol, w-butanol, or isobutanol biosynthetic capability. For example, a non- naturally occurring microbial organism having isopropanol, «-butanol, or isobutanol biosynthetic pathway can comprise at least two exogenous nucleic acids encoding desired enzymes or proteins. Thus, it is understood that any combination of two or more enzymes or proteins of a biosynthetic pathway can be included in a non-naturally occurring microbial organism of the invention. Similarly, it is understood that any combination of three or more enzymes or proteins of a biosynthetic pathway can be included in a non-naturally occurring microbial organism of the invention and so forth, as desired, so long as the combination of enzymes and/or proteins of the desired biosynthetic pathway results in production of the corresponding desired product. Similarly, any combination of four, or more enzymes or proteins of a biosynthetic pathway as disclosed herein can be included in a non-naturally occurring microbial organism of the invention, as desired, so long as the combination of enzymes and/or proteins of the desired biosynthetic pathway results in production of the corresponding desired product.

In addition to the biosynthesis of isopropanol, «-butanol, or isobutanol as described herein, the non-naturally occurring microbial organisms and methods of the invention also can be utilized in various combinations with each other and with other microbial organisms and methods well known in the art to achieve product biosynthesis by other routes. For example, one alternative to produce isopropanol, «-butanol, or isobutanol other than use of the isopropanol, «-butanol, or isobutanol producers is through addition of another microbial organism capable of converting isopropanol, «-butanol, or isobutanol pathway intermediate to isopropanol, «-butanol, or isobutanol. One such procedure includes, for example, the fermentation of a microbial organism that produces isopropanol, «-butanol, or isobutanol pathway intermediate. The isopropanol, n- butanol, or isobutanol pathway intermediate can then be used as a substrate for a second microbial organism that converts the isopropanol, «-butanol, or isobutanol pathway intermediate to isopropanol, «-butanol, or isobutanol. The isopropanol, «-butanol, or isobutanol pathway intermediate can be added directly to another culture of the second organism or the original culture of the isopropanol, w-butanol, or isobutanol pathway intermediate producers can be depleted of these microbial organisms by, for example, cell separation, and then subsequent addition of the second organism to the fermentation broth can be utilized to produce the final product without intermediate purification steps.

In other embodiments, the non-naturally occurring microbial organisms and methods of the invention can be assembled in a wide variety of subpathways to achieve biosynthesis of, for example, isopropanol, w-butanol, or isobutanol. In these embodiments, biosynthetic pathways for a desired product of the invention can be segregated into different microbial organisms, and the different microbial organisms can be co-cultured to produce the final product. In such a biosynthetic scheme, the product of one microbial organism is the substrate for a second microbial organism until the final product is synthesized. For example, the biosynthesis of isopropanol, «-butanol, or isobutanol can be accomplished by constructing a microbial organism that contains biosynthetic pathways for conversion of one pathway intermediate to another pathway intermediate or the product. Alternatively, isopropanol, «-butanol, or isobutanolalso can be biosynthetically produced from microbial organisms through co-culture or co- fermentation using two organisms in the same vessel, where the first microbial organism produces isopropanol, «-butanol, or isobutanol intermediate and the second microbial organism converts the intermediate to isopropanol, w-butanol, or isobutanol.

Given the teachings and guidance provided herein, those skilled in the art will understand that a wide variety of combinations and permutations exist for the non-naturally occurring microbial organisms and methods of the invention together with other microbial organisms, with the co- culture of other non-naturally occurring microbial organisms having subpathways and with combinations of other chemical and/or biochemical procedures well known in the art to produce isopropanol, w-butanol, or isobutanol.

Sources of encoding nucleic acids for isopropanol, w-butanol, or isobutanol pathway enzyme or protein can include, for example, any species where the encoded gene product is capable of catalyzing the referenced reaction. Such species include both prokaryotic and eukaryotic organisms including, but not limited to, bacteria, including archaea and eubacteria, and eukaryotes, including yeast, plant, insect, animal, and mammal, including human. Exemplary species for such sources include, for example, Escherichia coli, as well as other exemplary species disclosed herein or available as source organisms for corresponding genes. However, with the complete genome sequence available for now more than 550 species (with more than half of these available on public databases such as the NCBI), including 395 microorganism genomes and a variety of yeast, fungi, plant, and mammalian genomes, the identification of genes encoding the requisite isopropanol, w-butanol, or isobutanol biosynthetic activity for one or more genes in related or distant species, including for example, homologues, orthologs, paralogs and nonorthologous gene displacements of known genes, and the interchange of genetic alterations between organisms is routine and well known in the art. Accordingly, the metabolic alterations enabling biosynthesis of isopropanol, «-butanol, or isobutanol described herein with reference to a particular organism such as E. coli can be readily applied to other microorganisms, including prokaryotic and eukaryotic organisms alike. Given the teachings and guidance provided herein, those skilled in the art will know that a metabolic alteration exemplified in one organism can be applied equally to other organisms.

In some instances, such as when an alternative isopropanol, w-butanol, or isobutanol biosynthetic pathway exists in an unrelated species, isopropanol, w-butanol, or isobutanol biosynthesis can be conferred onto the host species by, for example, exogenous expression of a paralog or paralogs from the unrelated species that catalyzes a similar, yet non-identical metabolic reaction to replace the referenced reaction. Because certain differences among metabolic networks exist between different organisms, those skilled in the art will understand that the actual gene usage between different organisms can differ. However, given the teachings and guidance provided herein, those skilled in the art also will understand that the teachings and methods of the invention can be applied to all microbial organisms using the cognate metabolic alterations to those exemplified herein to construct a microbial organism in a species of interest that will synthesize isopropanol, w-butanol, or isobutanol.

Host microbial organisms can be selected from, and the non-naturally occurring microbial organisms generated in, for example, bacteria, yeast, fungus or any of a variety of other microorganisms applicable to fermentation processes. Exemplary bacteria include species selected from Escherichia coli, Klebsiella oxytoca, Anaerobiospirillum succiniciproducens, Actinobacillus succinogenes, Mannheimia succiniciproducens, Rhizobium etli, Bacillus subtilis, Corynebacterium glutamicum, Gluconobacter oxydans, Zymomonas mobilis, Lactococcus lactis, Lactobacillus plantarum, Streptomyces coelicolor, Clostridium acetobutylicum, Pseudomonas fluorescens, and Pseudomonas putida. Exemplary yeasts or fungi include species selected from Saccharomyces cerevisiae, Schizosaccharomyces pombe, Kluyveromyces lactis, Kluyveromyces marxianus, Aspergillus terreus, Aspergillus niger and Pichia pastoris . E. coli is a particularly useful host organism since it is a well characterized microbial organism suitable for genetic engineering. Other particularly useful host organisms include yeast such as Saccharomyces cerevisiae.

Methods for constructing and testing the expression levels of a non-naturally occurring isopropanol, w-butanol, or isobutanol-producing host can be performed, for example, by recombinant and detection methods well known in the art. Such methods can be found described in, for example, Sambrook et al., Molecular Cloning: A Laboratory Manual, Third Ed., Cold Spring Harbor Laboratory, New York (2001); and Ausubel et al., Current Protocols in Molecular Biology, John Wiley and Sons, Baltimore, MD (1999).

Exogenous nucleic acid sequences involved in a pathway for production of isopropanol, n- butanol, or isobutanol can be introduced stably or transiently into a host cell using techniques well known in the art including, but not limited to, conjugation, electroporation, chemical transformation, transduction, transfection, and ultrasound transformation. For exogenous expression in E. coli or other prokaryotic cells, some nucleic acid sequences in the genes or cDNAs of eukaryotic nucleic acids can encode targeting signals such as an w-terminal mitochondrial or other targeting signal, which can be removed before transformation into prokaryotic host cells, if desired. For example, removal of a mitochondrial leader sequence led to increased expression in is. coli (Hoffmeister et al., J. Biol. Chem. 280:4329-4338 (2005)). For exogenous expression in yeast or other eukaryotic cells, genes can be expressed in the cytosol without the addition of leader sequence, or can be targeted to mitochondrion or other organelles, or targeted for secretion, by the addition of a suitable targeting sequence such as a mitochondrial targeting or secretion signal suitable for the host cells. Thus, it is understood that appropriate modifications to a nucleic acid sequence to remove or include a targeting sequence can be incorporated into an exogenous nucleic acid sequence to impart desirable properties. Furthermore, genes can be subjected to codon optimization with techniques well known in the art to achieve optimized expression of the proteins.

An expression vector or vectors can be constructed to include one or more isopropanol, n- butanol, or isobutanol biosynthetic pathway encoding nucleic acids as exemplified herein operably linked to expression control sequences functional in the host organism. Expression vectors applicable for use in the microbial host organisms of the invention include, for example, plasmids, phage vectors, viral vectors, episomes and artificial chromosomes, including vectors and selection sequences or markers operable for stable integration into a host chromosome. Additionally, the expression vectors can include one or more selectable marker genes and appropriate expression control sequences. Selectable marker genes also can be included that, for example, provide resistance to antibiotics or toxins, complement auxotrophic deficiencies, or supply critical nutrients not in the culture media. Expression control sequences can include constitutive and inducible promoters, transcription enhancers, transcription terminators, and the like which are well known in the art. When two or more exogenous encoding nucleic acids are to be co-expressed, both nucleic acids can be inserted, for example, into a single expression vector or in separate expression vectors. For single vector expression, the encoding nucleic acids can be operationally linked to one common expression control sequence or linked to different expression control sequences, such as one inducible promoter and one constitutive promoter. The transformation of exogenous nucleic acid sequences involved in a metabolic or synthetic pathway can be confirmed using methods well known in the art. Such methods include, for example, nucleic acid analysis such as Northern blots or polymerase chain reaction (PCR) amplification of mRNA, or immunob lotting for expression of gene products, or other suitable analytical methods to test the expression of an introduced nucleic acid sequence or its corresponding gene product. It is understood by those skilled in the art that the exogenous nucleic acid is expressed in a sufficient amount to produce the desired product, and it is further understood that expression levels can be optimized to obtain sufficient expression using methods well known in the art and as disclosed herein.

The invention provides a method for producing isopropanol, w-butanol, or isobutanol that includes culturing the non-naturally occurring microbial organism disclosed herein, under conditions and for a sufficient period of time to produce isopropanol, w-butanol, or isobutanol, including organisms that incorporate one, two, three, four, five, six, seven, eight, up to all exogenous nucleic acids encoding enzymes that complete a isopropanol, «-butanol, or isobutanol pathway. In some embodiments, at least one exogenous nucleic acid is a heterologous nucleic acid. Finally, culturing of thenon-naturally occurring microbial organisms of the invention can be performed in a substantially anaerobic culture medium.

Suitable purification and/or assays to test for the production of isopropanol, w-butanol, or isobutanol can be performed using well known methods. Suitable replicates such as triplicate cultures can be grown for each engineered strain to be tested. For example, product and byproduct formation in the engineered production host can be monitored. The final product and intermediates, and other organic compounds, can be analyzed by methods such as HPLC (High Performance Liquid Chromatography), GC-MS (Gas Chromatography-Mass Spectroscopy) and LC-MS (Liquid Chromatography-Mass Spectroscopy) or other suitable analytical methods using routine procedures well known in the art. The release of product in the fermentation broth can also be tested with the culture supernatant. Byproducts and residual glucose can be quantified by HPLC using, for example, a refractive index detector for glucose and alcohols, and a UV detector for organic acids (Lin et al., Biotechnol. Bioeng. 90:775-779 (2005)), or other suitable assay and detection methods well known in the art. The individual enzyme or protein activities from the exogenous DNA sequences can also be assayed using methods well known in the art (see, for example, WO/2008/115840 and Hanai et al., Appl Environ Microbiol 73:7814-7818 (2007)).

The isopropanol, «-butanol, or isobutanol can be separated from other components in the culture using a variety of methods well known in the art. Such separation methods include, for example, extraction procedures as well as methods that include continuous liquid-liquid extraction, pervaporation, membrane filtration, membrane separation, reverse osmosis, electrodialysis, distillation, crystallization, centrifugation, extractive filtration, ion exchange chromatography, size exclusion chromatography, adsorption chromatography, and ultrafiltration. All of the above methods are well known in the art. Any of the non- naturally occurring microbial organisms described herein can be cultured to produce and/or secrete the biosynthetic products of the invention. For example, the isopropanol, «-butanol, or isobutanol producers can be cultured for the biosynthetic production of isopropanol, w-butanol, or isobutanol.

For the production of isopropanol, w-butanol, or isobutanol, the recombinant strains are cultured in a medium with carbon source and other essential nutrients. It is highly desirable to maintain anaerobic conditions in the fermenter to reduce the cost of the overall process. Such conditions can be obtained, for example, by first sparging the medium with nitrogen and then sealing the flasks with a septum and crimp-cap. For strains where growth is not observed anaerobically, microaerobic conditions can be applied by perforating the septum with a small hole for limited aeration. Exemplary anaerobic conditions have been described previously and are well-known in the art. Exemplary aerobic and anaerobic conditions are described, for example, in United State Patent application serial No. 11/891,602, filed August 10, 2007. Fermentations can be performed in a batch, fed-batch or continuous manner, as disclosed herein.

If desired, the pH of the medium can be maintained at a desired pH, in particular neutral pH, such as a pH of around 7 by addition of a base, such as NaOH or other bases, or acid, as needed to maintain the culture medium at a desirable pH. The growth rate can be determined by measuring optical density using a spectrophotometer (600 nm), and the glucose uptake rate by monitoring carbon source depletion over time.

In addition to renewable feedstocks such as those exemplified above, the isopropanol, w-butanol, or isobutanol microbial organisms of the invention also can be modified for growth on syngas as its source of carbon. In this specific embodiment, one or more proteins or enzymes are expressed in the isopropanol, w-butanol, or isobutanol producing organisms to provide a metabolic pathway for utilization of syngas or other gaseous carbon source.

Organisms of the present invention can utilize, for example, any carbohydrate source which can supply a source of carbon to the non-naturally occurring microorganism. Such sources include, for example, sugars such as glucose, xylose, arabinose, galactose, mannose, fructose and starch. Other sources of carbohydrate include, for example, renewable feedstocks and biomass. Exemplary types of biomasses that can be used as feedstocks in the methods of the invention include cellulosic biomass, hemicellulosic biomass and lignin feedstocks or portions of feedstocks. Such biomass feedstocks contain, for example, carbohydrate substrates useful as carbon sources such as glucose, xylose, arabinose, galactose, mannose, fructose and starch. Given the teachings and guidance provided herein, those skilled in the art will understand that renewable feedstocks and biomass other than those exemplified above also can be used for culturing the microbial organisms of the invention for the production of isopropanol, «-butanol, or isobutanol.

Accordingly, given the teachings and guidance provided herein, those skilled in the art will understand that a non-naturally occurring microbial organism can be produced that secretes the biosynthesized compounds of the invention when grown on a carbon source such as , syngas, CO and/or CO2. Such compounds include, for example, isopropanol, w-butanol, or isobutanol and any of the intermediate metabolites in the isopropanol, w-butanol, or isobutanol pathway. All that is required is to engineer in one or more of the required enzyme or protein activities to achieve biosynthesis of the desired compound or intermediate including, for example, inclusion of some or all of the isopropanol, w-butanol, or isobutanol biosynthetic pathways. Accordingly, the invention provides a non-naturally occurring microbial organism that produces and/or secretes isopropanol, w-butanol, or isobutanol when grown on a carbohydrate or other carbon source and produces and/or secretes any of the intermediate metabolites shown in the isopropanol, w-butanol, or isobutanol pathway when grown on a carbohydrate or other carbon source. The isopropanol, «-butanol, or isobutanol producing microbial organisms of the invention can initiate synthesis from an intermediate, for example, acetyl-CoA.

The non-naturally occurring microbial organisms of the invention are constructed using methods well known in the art as exemplified herein to exogenously express at least one nucleic acid encoding an isopropanol, w-butanol, or isobutanol pathway enzyme or protein in sufficient amounts to produce isopropanol, w-butanol, or isobutanol. It is understood that the microbial organisms of the invention are cultured under conditions sufficient to produce isopropanol, n- butanol, or isobutanol. Following the teachings and guidance provided herein, the non-naturally occurring microbial organisms of the invention can achieve biosynthesis of isopropanol, n- butanol, or isobutanol resulting in intracellular concentrations between about 0.1-2000 mM or more. Generally, the intracellular concentration of isopropanol, «-butanol, or isobutanol is between about 3-1800 mM, particularly between about 5-1700 mM and more particularly between about 8-1600 mM, including about 100 mM, 200 mM, 500 mM, 800 mM, or more. Intracellular concentrations between and above each of these exemplary ranges also can be achieved from the non-naturally occurring microbial organisms of the invention.

In some embodiments, culture conditions include anaerobic or substantially anaerobic growth or maintenance conditions. Exemplary anaerobic conditions have been described previously and are well known in the art. Exemplary anaerobic conditions for fermentation processes are described herein and are described, for example, in U.S. patent application No. US 2009/0047719, filed August 10, 2007. Any of these conditions can be employed with the non- naturally occurring microbial organisms as well as other anaerobic conditions well known in the art. Under such anaerobic conditions, the isopropanol, «-butanol, or isobutanol producers can synthesize isopropanol, «-butanol, or isobutanol at intracellular concentrations of 5-10 mM or more as well as all other concentrations exemplified herein. It is understood that, even though the above description refers to intracellular concentrations, isopropanol, «-butanol, or isobutanol producing microbial organisms can produce isopropanol, «-butanol, or isobutanol intracellular Iy and/or secrete the product into the culture medium.

The culture conditions can include, for example, liquid culture procedures as well as fermentation and other large scale culture procedures. As described herein, particularly useful yields of the biosynthetic products of the invention can be obtained under anaerobic or substantially anaerobic culture conditions.

As described herein, one exemplary growth condition for achieving biosynthesis of isopropanol, «-butanol, or isobutanol includes anaerobic culture or fermentation conditions. In certain embodiments, the non-naturally occurring microbial organisms of the invention can be sustained, cultured or fermented under anaerobic or substantially anaerobic conditions. Briefly, anaerobic conditions refers to an environment devoid of oxygen. Substantially anaerobic conditions include, for example, a culture, batch fermentation or continuous fermentation such that the dissolved oxygen concentration in the medium remains between 0 and 10% of saturation. Substantially anaerobic conditions also includes growing or resting cells in liquid medium or on solid agar inside a sealed chamber maintained with an atmosphere of less than 1% oxygen. The percent of oxygen can be maintained by, for example, sparging the culture with an N2/CO2 mixture or other suitable non-oxygen gas or gases.

The culture conditions described herein can be scaled up and grown continuously for manufacturing of isopropanol, w-butanol, or isobutanol. Exemplary growth procedures include, for example, fed-batch fermentation and batch separation; fed-batch fermentation and continuous separation, or continuous fermentation and continuous separation. All of these processes are well known in the art. Fermentation procedures are particularly useful for the biosynthetic production of commercial quantities of isopropanol, w-butanol, or isobutanol. Generally, and as with non-continuous culture procedures, the continuous and/or near- continuous production of isopropanol, w-butanol, or isobutanol will include culturing a non- naturally occurring isopropanol, «-butanol, or isobutanol producing organism of the invention in sufficient nutrients and medium to sustain and/or nearly sustain growth in an exponential phase. Continuous culture under such conditions can be include, for example, 1 day, 2, 3, 4, 5, 6 or 7 days or more. Additionally, continuous culture can include 1 week, 2, 3, 4 or 5 or more weeks and up to several months. Alternatively, organisms of the invention can be cultured for hours, if suitable for a particular application. It is to be understood that the continuous and/or near- continuous culture conditions also can include all time intervals in between these exemplary periods. It is further understood that the time of culturing the microbial organism of the invention is for a sufficient period of time to produce a sufficient amount of product for a desired purpose.

Fermentation procedures are well known in the art. Briefly, fermentation for the biosynthetic production of isopropanol, w-butanol, or isobutanol can be utilized in, for example, fed-batch fermentation and batch separation; fed-batch fermentation and continuous separation, or continuous fermentation and continuous separation. Examples of batch and continuous fermentation procedures are well known in the art.

In addition to the above fermentation procedures using the isopropanol, «-butanol, or isobutanol producers of the invention for continuous production of substantial quantities of isopropanol, n- butanol, or isobutanol, the isopropanol, w-butanol, or isobutanol producers also can be, for example, simultaneously subjected to chemical synthesis procedures to convert the product to other compounds or the product can be separated from the fermentation culture and sequentially subjected to chemical conversion to convert the product to other compounds, if desired.

In some embodiments, syngas can be used as a carbon feedstock. Important process considerations for a syngas fermentation are high biomass concentration and good gas-liquid mass transfer (Bredwell et al, Biotechnol Prog. 15:834-844 (1999). The solubility of CO in water is somewhat less than that of oxygen. Continuously gas-sparged fermentations can be performed in controlled fermenters with constant off-gas analysis by mass spectrometry and periodic liquid sampling and analysis by GC and HPLC. The liquid phase can function in batch mode. Fermentation products such as alcohols, organic acids, and residual glucose along with residual methanol are quantified by HPLC (Shimadzu, Columbia MD), for example, using an Aminex® series of HPLC columns (for example, HPX-87 series) (BioRad, Hercules CA), using a refractive index detector for glucose and alcohols, and a UV detector for organic acids. The growth rate is determined by measuring optical density using a spectrophotometer (600 nm). All piping in these systems is glass or metal to maintain anaerobic conditions. The gas sparging is performed with glass frits to decrease bubble size and improve mass transfer. Various sparging rates are tested, ranging from about 0.1 to 1 wm (vapor volumes per minute). To obtain accurate measurements of gas uptake rates, periodic challenges are performed in which the gas flow is temporarily stopped, and the gas phase composition is monitored as a function of time.

In order to achieve the overall target productivity, methods of cell retention or recycle are employed. One method to increase the microbial concentration is to recycle cells via a tangential flow membrane from a sidestream. Repeated batch culture can also be used, as previously described for production of acetate by Moorella (Sakai et al., J Biosci.Bioeng 99:252- 258 (2005)). Various other methods can also be used (Bredwell et al., Biotechnol Prog. 15:834- 844 (1999); Datar et al., Biotechnol Bioeng 86:587-594 (2004)). Additional optimization can be tested such as overpressure at 1.5 atm to improve mass transfer (Najafpour and Younesi, Enzyme and Microbial Technology 38[l-2], 223-228 (2006)).

Once satisfactory performance is achieved using pure H2/CO as the feed, synthetic gas mixtures are generated containing inhibitors likely to be present in commercial syngas. For example, a typical impurity profile is 4.5% CH₄, 0.1% C₂H₂, 0.35% C₂H₆, 1.4% C₂H₄, and 150 ppm nitric oxide (Datar et al., Biotechnol Bioeng 86:587-594 (2004)). Tars, represented by compounds such as benzene, toluene, ethylbenzene, p-xylene, o-xylene, and naphthalene, are added at ppm levels to test for any effect on production. For example, it has been shown that 40 ppm NO is inhibitory to C. carboxidivorans (Ahmed and Lewis, Biotechnol Bioeng 97:1080-1086 (2007)). Cultures are tested in shake-flask cultures before moving to a fermentor. Also, different levels of these potential inhibitory compounds are tested to quantify the effect they have on cell growth. This knowledge is used to develop specifications for syngas purity, which is utilized for scale up studies and production. If any particular component is found to be difficult to decrease or remove from syngas used for scale up, an adaptive evolution procedure is utilized to adapt cells to tolerate one or more impurities.

Advances in the field of protein engineering make it feasible to alter any of the enzymes disclosed herein to act efficiently on substrates not known to be natural to them. Below are several examples of broad-specificity enzymes from diverse classes of interest and and methods that have been used for evolving such enzymes to act on non-natural substrates.

One class of enzymes in the pathways disclosed herein is the oxidoreductases that interconvert ketones or aldehydes to alcohols (1.1.1). Enzymes in this class that can operate on a wide range of substrates. An alcohol dehydrogenase (1.1.1.1) purified from the soil bacterium Brevibacterium sp KU 1309 (Hirano et al., J. Biosci. Bioeng. 100:318-322 (2005)) was shown to operate on a plethora of aliphatic as well as aromatic alcohols with high activities. Table 34 shows the activity of the enzyme and its K_m on different alcohols. The enzyme is reversible and has very high activity on several aldehydes also as shown in Table 34.

Table 34

In this Table, the activity of 2-phenylethanol, corresponding to 19.2 U/mg, was taken as 100%.

Table 35

Lactate dehydrogenase (1.1.1.27) from Ralstonia eutropha is another enzyme that has been demonstrated to have high activities on several 2-oxoacids such as 2-oxobutyrate, 2- oxopentanoate and 2-oxoglutarate (a C5 compound analogous to 2-oxoadipate) (Steinbuchel et al., Eur. J. Biochem. 130:329-334 (1983)). Column 2 in Table 36 shows the activities oϊldhA from R. eutropha (formerly A eutrophus) on different substrates (Steinbuchel et al., supra). Table 36

Oxidoreductases that can convert 2-oxoacids to their acyl-CoA counterparts (1.2.1) have been shown to accept multiple substrates as well. For example, branched-chain 2-keto-acid dehydrogenase complex (BCKAD), also known as 2-oxoisovalerate dehydrogenase (1.2.1.25), participates in branched-chain amino acid degradation pathways, converting 2-keto acids derivatives of valine, leucine and isoleucine to their acyl-CoA derivatives and CO2. In some organisms including Rattus norvegicus (Paxton et al., Biochem. J. 234:295-303 (1986)) and Saccharomyces cerevisiae (Sinclair et al., Biochem. MoI. Biol. Int. 31 :911-922 (1993)), this complex has been shown to have a broad substrate range that includes linear oxo-acids such as 2-oxobutanoate and alpha-ketoglutarate, in addition to the branched-chain amino acid precursors.

CoA transferases (2.8.3) have been demonstrated to have the ability to act on more than one substrate. Specifically, a CoA transferase was purified from Clostridium acetobutylicum and was reported to have the highest activities on acetate, propionate, and butyrate. It also had significant activities with valerate, isobutyrate, and crotonate (Wiesenborn et al., Appl. Environ. Microbiol. 55:323-329 (1989)). In another study, the E. coli enzyme acyl-CoA:acetate-CoA transferase, also known as acetate-CoA transferase (EC 2.8.3.8), has been shown to transfer the CoA moiety to acetate from a variety of branched and linear acyl-CoA substrates, including isobutyrate (Matthies et al., Appl Environ Microbiol 58:1435-1439 (1992)), valerate (Vanderwinkel et al., Biochem. Biophys. Res Commun. 33:902-908 (1968)) and butanoate (Vanderwinkel et al., supra).

The argument for broad substrate specificity of the enzymes is further strengthened as we explore other enzyme classes. Some isomerases (5.3.3) have also been proven to operate on multiple substrates. For example, L-rhamnose isomerase from Pseudomonas stutzeri catalyzes the isomerization between various aldoalses and ketoses (Yoshida et al., J. MoI. Biol. 365:1505- 1516 (2007)). These include isomerization between L-rhamnose and L-rhamnulose, L-mannose and L-fructose, L-xylose and L-xylulose, D-ribose and D-ribulose, and D-allose and D-psicose.

In yet another class of enzymes, the phosphotransferases (2.7.1), the homoserine kinase (2.7.1.39) from E. coli that converts L-homoserine to L-homoserine phosphate, was found to phosphorylate numerous homoserine analogs. In these substrates, the carboxyl functional group at the R-position had been replaced by an ester or by a hydroxymethyl group (Huo et al., Biochemistry 35: 16180-16185 (1996)). Table 37 demonstrates the broad substrate specificity of this kinase.

Table 37

Another class of enzymes that we have encountered in the proposed pathways is the acid-thiol ligases (6.2.1). Like enzymes in other classes, certain enzymes in this class have been determined to have broad substrate specificity. For example, acyl CoA ligase from Pseudomonas putida has been demonstrated to work on several aliphatic substrates including acetic, propionic, butyric, valeric, hexanoic, heptanoic, and octanoic acids and on aromatic compounds such as phenylacetic and phenoxyacetic acids (Fernandez-Valverde et al, Appl. Environ. Microbiol. 59: 1149-1154 (1993)). A related enzyme, malonyl CoA synthetase (6.3.4.9) from Rhizobium trifolii could convert several diacids, namely, ethyl-, propyl-, allyl-, isopropyl-, dimethyl-, cyclopropyl-, cyclopropylmethylene-, cyclobutyl-, and benzyl-malonate into their corresponding monothio esters (Pohl et al., J. Am. Chem. Soc. 123:5822-5823 (2001)). In a similar vein, we also found decarboxylases (4.1.1) with broad substrate ranges. Pyruvate decarboxylase (PDC), also termed keto-acid decarboxylase, is a key enzyme in alcoholic fermentation, catalyzing the decarboxylation of pyruvate to acetaldehyde. The enzyme isolated from Saccharomyces cerevisiae has a broad substrate range for aliphatic 2-keto acids including 2-ketobutyrate, 2-keto valerate, and 2-phenylpyruvate (Li et al., Biochemistry 38:10004-10012 (1999)). Similarly, benzoylformate decarboxylase has a broad substrate range and has been the target of enzyme engineering studies. The enzyme from Pseudomonas putida has been extensively studied and crystal structures of this enzyme are available (Polovnikova et al., Biochemistry 42: 1820-1830 (2003);Hasson et al., Biochemistry 37:9918-9930 (1998)). Branched chain alpha-ketoacid decarboxylase (BCKA) has been shown to act on a variety of compounds varying in chain length from 3 to 6 carbons (Oku et al., J Biol Chem. 263:18386-18396 (1988)). The enzyme in Lactococcus lactis has been characterized on a variety of branched and linear substrates including 2-oxobutanoate, 2-oxohexanoate, 2-oxopentanoate, 3-methyl-2- oxobutanoate, 4-methyl-2-oxobutanoate and isocaproate (Smit et al., Appl Environ Microbiol 71 :303-311 (2005)).

Interestingly, enzymes known to have one dominant activity have also been indicated to catalyze a very different function. For example, the cofactor-dependent phosphoglycerate mutase (5.4.2.1) from Bacillus stearothermophilus and Bacillus subtilis is known to function as a phosphatase as well (Rigden et al., Protein ScL 10:1835-1846 (2001)). The enzyme from B. stearothermophilus is known to have activity on several substrates, including 3- phosphoglycerate, alpha-napthylphosphate, p-nitrophenylphosphate, AMP, fructose-6- phosphate, ribose-5 -phosphate and CMP. In contrast to these examples, where the enzymes naturally have broad substrate specificities, numerous enzymes have been modified using directed evolution to broaden their specificity towards their non-natural substrates. Alternatively, the substrate preference of an enzyme has also been changed using directed evolution. The success of these methods has been well- documented in literature and reinforces our claim that it is more than feasible to engineer a given enzyme for efficient function on a natural/non-natural substrate. For example, it has been reported that the enantioselectivity of a lipase from Pseudomonas aeruginosa was improved significantly. This enzyme hydrolyzed p-nitrophenyl 2-methyldecanoate with only 2% enantiomeric excess (ee) in favor of the (S)-Αciά. However, after four successive rounds of error- prone mutagenesis and screening, a variant was produced that catalyzed the requisite reaction with 81% ee (Reetz et al, Angew. Chem. Int. Ed Engl. 36:2830-2832 (1997)).

As mentioned earlier, directed evolution methods have enabled the modification of an enzyme to function on an array of substrates not natural to it. The substrate specificity of the lipase in P. aeruginosa was broadened by randomization of amino acid residues near the active site. This allowed for the acceptance of alpha-substituted carboxylic acid esters by this enzyme (Reetz et al., Angew. Chem. Int. Ed Engl. 44:4192-4196 (2005)). In another successful attempt, DNA shuffling was employed to create an Escherichia coli aminotransferase that accepted β-branched substrates, which were poorly accepted by the wild -type enzyme (Yano et al., Proc. Natl. Acad. ScL U. S. A 95:5511-5515 (1998)). Specifically, at the end of four rounds of shuffling, the activity of aspartate aminotransferase for valine and 2-oxovaline increased by up to five orders of magnitude, while decreasing the activity towards the natural substrate, aspartate, by up to 30- fold. In a very recent report in Science, an algorithm was used to design a retro-aldolase that could be used to catalyze the carbon-carbon bond cleavage in a non-natural and non-biological substrate, 4-hydroxy-4-(6-methoxy-2-naphthyl)-2-butanone. These algorithms used different combinations of four different catalytic motifs to design new enzymes and 20 of the selected designs for experimental characterization had four-fold improved rates over the uncatalyzed reaction (Jiang et al., Science 319:1387-1391 (2008)). Thus, not only are these engineering approaches capable of expanding the array of substrates on which an enzyme can act, but they enable the design and construction of very efficient enzymes. For example, a method of DNA shuffling (random chimeragenesis on transient templates or RACHITT) was reported to lead to an engineered monooxygenase that had an improved rate of desulfurization on complex substrates as well as 20-fold faster conversion of a non-natural substrate (Coco et al. Nat. Biotechnol. 19:354-359 (2001)). Similarly, the specific activity of a sluggish mutant triosephosphate isomerase enzyme was improved up to 19-fold from 1.3 fold (Hermes et al., Proc. Natl. Acad. Sci. U. S. A 87:696-700 (1990)). This enhancement in specific activity was accomplished by using random mutagenesis over the whole length of the protein and the improvement could be traced back to mutations in six amino acid residues.

The effectiveness of protein engineering approaches to alter the substrate specificity of an enzyme for a desired substrate has also been demonstrated in several studies. Isopropylmalate dehydrogenase from Thermus thermophilus was modified by changing residues close to the active site so that it could now act on malate and D-lactate as substrates (Fujita et al., Biosci. Biotechnol Biochem. 65:2695-2700 (2001)). In this study as well as in others, it was pointed out that one or a few residues could be modified to alter the substrate specificity. A case in point is the dihydroflavonol 4-reductase for which a single amino acid was changed in the presumed substrate-binding region that could preferentially reduce dihydrokaempferol (Johnson et al., Plant J. 25:325-333 (2001)). The substrate specificity of a very specific isocitrate dehydrogenase from Escherichia coli was changed form isocitrate to isopropylmalate by changing one residue in the active site (Doyle et al., Biochemistry 40:4234-4241 (2001)). In a similar vein, the cofactor specificity of a NAD -dependent 1 ,5-hydroxyprostaglandin dehydrogenase was altered to NADP⁺ by changing a few residues near the N-terminal end (Cho et al., Arch. Biochem. Biophys. 419: 139-146 (2003)). Sequence analysis and molecular modeling analysis were used to identify the key residues for modification, which were further studied by site-directed mutagenesis.

There are many other examples spanning diverse classes of enzymes where the function of enzyme was changed to favor one non-natural substrate over the natural substrate of the enzyme. A fucosidase was evolved from a galactosidase in E. coli by DNA shuffling and screening (Zhang et al., Proc Natl Acad Sci US. A 94:4504-4509 (1997)). Similarly, aspartate aminotransferase from E. coli was converted into a tyrosine aminotransferase using homology modeling and site-directed mutagenesis (Onuffer et al., Protein Sci. 4:1750-1757 (1995)). Site- directed mutagenesis of two residues in the active site of benzoylformate decarboxylase from P. putida reportedly altered the affinity (K_m) towards natural and non-natural substrates (Siegert et al., Protein Eng Des SeI 18:345-357 (2005)). Cytochrome c peroxidase (CCP) from Saccharomyces cerevisiae was subjected to directed molecular evolution to generate mutants with increased activity against the classical peroxidase substrate guaiacol, thus changing the substrate specificity of CCP from the protein cytochrome c to a small organic molecule. After three rounds of DNA shuffling and screening, mutants were isolated which possessed a 300-fold increased activity against guaiacol and up to 1000-fold increased specificity for this substrate relative to that for the natural substrate (Iffland et al., Biochemistry 39:10790-10798 (2000)).

In some cases, enzymes with different substrate preferences than both the parent enzymes have been obtained. For example, biphenyl-dioxygenase-mediated degradation of polychlorinated biphenyls was improved by shuffling genes from two bacteria, Pseudomonas pseudoalcaligens and Burkholderia cepacia (Kumamaru et al., Nat. Biotechnol 16, 663-666 (1998)). The resulting chimeric biphenyl oxygenases showed different substrate preferences than both the parental enzymes and enhanced the degradation activity towards related biphenyl compounds and single aromatic ring hydrocarbons such as toluene and benzene which were originally poor substrates for the enzyme.

It is not only possible to change the enzyme specificity, but also to enhance the activities on those substrates on which the enzymes naturally have low activities. One study demonstrated that amino acid racemase from P. putida that had broad substrate specificity (on lysine, arginine, alanine, serine, methionine, cysteine, leucine and histidine among others) but low activity towards tryptophan could be improved significantly by random mutagenesis ( Kino et al., Appl. Microbiol. Biotechnol. 73 :1299-1305 (2007)). Similarly, the active site of the bovine BCKAD was engineered to favor alternate substrate acetyl-CoA ( Meng et al. Biochemistry 33 :12879- 12885 (1994)). An interesting aspect of these approaches is that even if random methods have been applied to generate these mutated enzymes with efficacious activities, the exact mutations or structural changes that confer the improvement in activity can be identified. For example, in the aforementioned study, the mutations that facilitated improved activity on tryptophan could be traced back to two different positions.

Directed evolution has also been used to express proteins that are difficult to express. For example, by subjecting the horseradish peroxidase to random mutagenesis and gene recombination, mutants could be extracted that had more than 14-fold activity than the wild type (Lin et al., Biotechnol. Prog. 15:467-471 (1999)).

A final example of directed evolution shows the extensive modifications to which an enzyme can be subjected to achieve a range of desired functions. The enzyme, lactate dehydrogenase from Bacillus stearothermophilus was subjected to site-directed mutagenesis, and three amino acid substitutions were made at sites that were believed to determine the specificity towards different hydroxyacids (Clarke et al., Biochem. Biophys. Res. Commun. 148:15-23 (1987)). After these mutations, the specificity for oxaloacetate over pyruvate was increased to 500 in contrast to the wild type enzyme that had a catalytic specificity for pyruvate over oxaloacetate of 1000. This enzyme was further engineered using site-directed mutagenesis to have activity towards branched-chain substituted pyruvates (Wilks et al., Biochemistry 29:8587-8591 (1990)). Specifically, the enzyme had a 55-fold improvement in K_cat for alpha-ketoisocaproate. Three structural modifications were made in the same enzyme to change its substrate specificity from lactate to malate. The enzyme was highly active and specific towards malate (Wilks et al., Science 242:1541-1544 (1988)). The same enzyme from B. stearothermophilus was subsequently engineered to have high catalytic activity towards alpha-keto acids with positively charged side chains, such as those containing ammonium groups (Hogan et al., Biochemistry 34:4225-4230 (1995)). Mutants with acidic amino acids introduced at position 102 of the enzyme favored binding of such side chain ammonium groups. The results obtained proved that the mutants showed up to 25 -fold improvements in kc_at/K_m values for omega-amino-alpha-keto acid substrates. This enzyme was also structurally modified to function as a phenyllactate dehydrogenase instead of a lactate dehydrogenase (Wilks et al., Biochemistry 31 :7802-7806 (1992)). Restriction sites were introduced into the gene for the enzyme which enabled a region of the gene to be excised. This region coded for a mobile surface loop of polypeptide (residues 98-110) which normally seals the active site vacuole from bulk solvent and is a major determinant of substrate specificity. The variable length and sequence loops were inserted into the cut gene and used to synthesize hydroxyacid dehydrogenases with altered substrate specificities. With one longer loop construction, activity with pyruvate was reduced one-million- fold but activity with phenylpyruvate was largely unaltered. A switch in specificity (k_cat/K_m) of 390,000-fold was achieved. The 1700:1 selectivity of this enzyme for phenylpyruvate over pyruvate is that required in a phenyllactate dehydrogenase.

As indicated above, directed evolution is a powerful approach that involves the introduction of mutations targeted to a specific gene in order to improve and/or alter the properties of an enzyme. Improved and/or altered enzymes can be identified through the development and implementation of sensitive high-throughput screening assays that allow the automated screening of many enzyme variants (e.g., >10⁴). Iterative rounds of mutagenesis and screening typically are performed to afford an enzyme with optimized properties. Computational algorithms that can help to identify areas of the gene for mutagenesis also have been developed and can significantly reduce the number of enzyme variants that need to be generated and screened. Numerous directed evolution technologies have been developed (for reviews, see Hibbert et al., Biomol.Eng 22:11-19 (2005); Huisman et al., Enzyme evolution for chemical process applications, p. 717-742 (2007)). In R. N. Patel (ed.), Biocatalysis in the pharmaceutical and biotechnology industries. CRC Press; Otten, et al., Biomol.Eng 22:1-9 (2005); and Sen et al., Appl Biochem.Biotechnol 143:212-223 (2007)) to be effective at creating diverse variant libraries and these methods have been successfully applied to the improvement of a wide range of properties across many enzyme classes.

Enzyme characteristics that have been improved and/or altered by directed evolution technologies include, for example, selectivity/specificity - for conversion of non-natural substrates; temperature stability - for robust high temperature processing; pH stability - for bioprocessing under lower or higher pH conditions; substrate or product tolerance - so that high product titers can be achieved; binding (K_m) - broadens substrate binding to include non-natural substrates; inhibition (K₁) - to remove inhibition by products, substrates, or key intermediates; activity (kcat) - increases enzymatic reaction rates to achieve desired flux; expression levels - increases protein yields and overall pathway flux; oxygen stability - for operation of air sensitive enzymes under aerobic conditions; and anaerobic activity - for operation of an aerobic enzyme in the absence of oxygen.

The following exemplary methods have been developed for the mutagenesis and diversification of genes to target desired properties of specific enzymes. Any of these can be used to alter/optimize activity of a decarboxylase enzyme.

EpPCR (Pritchard et al., J Theor.Biol 234:497-509 (2005)) introduces random point mutations by reducing the fidelity of DNA polymerase in PCR reactions by the addition Of Mn²⁺ ions, by biasing dNTP concentrations, or by other conditional variations. The five step cloning process to confine the mutagenesis to the target gene of interest involves: 1) error-prone PCR amplification of the gene of interest; 2) restriction enzyme digestion; 3) gel purification of the desired DNA fragment; 4) ligation into a vector; 5) transformation of the gene variants into a suitable host and screening of the library for improved performance. This method can generate multiple mutations in a single gene simultaneously, which can be useful. A high number of mutants can be generated by EpPCR, so a high-throughput screening assay or a selection method (especially using robotics) is useful to identify those with desirable characteristics.

Error-prone Rolling Circle Amplification (epRCA) (Fujii et al., Nucleic Acids Res 32:el45 (2004); and Fujii et al., Nat.Protoc. 1 :2493-2497 (2006)) has many of the same elements as epPCR except a whole circular plasmid is used as the template and random 6-mers with exonuclease resistant thiophosphate linkages on the last 2 nucleotides are used to amplify the plasmid followed by transformation into cells in which the plasmid is re-circularized at tandem repeats. Adjusting the Mn²⁺ concentration can vary the mutation rate somewhat. This technique uses a simple error-prone, single-step method to create a full copy of the plasmid with 3 - 4 mutations/kbp. No restriction enzyme digestion or specific primers are required. Additionally, this method is typically available as a kit.

DNA or Family Shuffling (Stemmer, W. P. Proc. Natl. Acad. Sci. U.S.A. 91 :10747-10751 (1994); and Stemmer, W. P. Nature 370:389-391 (1994)) typically involves digestion of 2 or more variant genes with nucleases such as Dnase I or EndoV to generate a pool of random fragments that are reassembled by cycles of annealing and extension in the presence of DNA polymerase to create a library of chimeric genes. Fragments prime each other and recombination occurs when one copy primes another copy (template switch). This method can be used with >lkbp DNA sequences. In addition to mutational recombinants created by fragment reassembly, this method introduces point mutations in the extension steps at a rate similar to error-prone PCR. The method can be used to remove deleterious random neutral mutations that might confer antigenicity.

Staggered Extension (StEP) (Zhao et al., Nat.Biotechnol 16:258-261 (1998)) entails template priming followed by repeated cycles of 2 step PCR with denaturation and very short duration of annealing/extension (as short as 5 sec). Growing fragments anneal to different templates and extend further, which is repeated until full-length sequences are made. Template switching means most resulting fragments have multiple parents. Combinations of low- fidelity polymerases (T aq and Mutazyme) reduce error-prone biases because of opposite mutational spectra.

In Random Priming Recombination (RPR) random sequence primers are used to generate many short DNA fragments complementary to different segments of the template. (Shao et al., Nucleic Acids Res., 26:681-683 (1998)). Base misincorporation and mispriming via epPCR give point mutations. Short DNA fragments prime one another based on homology and are recombined and reassembled into full-length by repeated thermocycling. Removal of templates prior to this step assures low parental recombinants. This method, like most others, can be performed over multiple iterations to evolve distinct properties. This technology avoids sequence bias, is independent of gene length, and requires very little parent DNA for the application. In Heteroduplex Recombination linearized plasmid DNA is used to form heteroduplexes that are repaired by mismatch repair. (Volkov et al., Nucleic Acids Res 27:el8 (1999); and Volkov et al., Methods Enzymol. 328:456-463 (2000)). The mismatch repair step is at least somewhat mutagenic. Heteroduplexes transform more efficiently than linear homoduplexes. This method is suitable for large genes and whole operons.

Random Chimeragenesis on Transient Templates (RACHITT) (Coco et al., Nat.Biotechnol 19:354-359 (2001)) employs Dnase I fragmentation and size fractionation of ssDNA. Homologous fragments are hybridized in the absence of polymerase to a complementary ssDNA scaffold. Any overlapping unhybridized fragment ends are trimmed down by an exonuclease. Gaps between fragments are filled in, and then ligated to give a pool of full-length diverse strands hybridized to the scaffold (that contains U to preclude amplification). The scaffold then is destroyed and is replaced by a new strand complementary to the diverse strand by PCR amplification. The method involves one strand (scaffold) that is from only one parent while the priming fragments derive from other genes; the parent scaffold is selected against. Thus, no reannealing with parental fragments occurs. Overlapping fragments are trimmed with an exonuclease. Otherwise, this is conceptually similar to DNA shuffling and StEP. Therefore, there should be no siblings, few inactives, and no unshuffled parentals. This technique has advantages in that few or no parental genes are created and many more crossovers can result relative to standard DNA shuffling.

Recombined Extension on Truncated templates (RETT) entails template switching of unidirectionally growing strands from primers in the presence of unidirectional ssDNA fragments used as a pool oftemplates. (Lee et al., J.Molec. Catalysis 26:119-129 (2003)). No DNA endonucleases are used. Unidirectional ssDNA is made by by DNA polymerase with random primers or serial deletion with exonuclease. Unidirectional ssDNA are only templates and not primers. Random priming and exonucleases don't introduce sequence bias as true of enzymatic cleavage of DNA shuffling/RACHITT. RETT can be easier to optimize than StEP because it uses normal PCR conditions instead of very short extensions. Recombination occurs as a component of the PCR steps— no direct shuffling. This method can also be more random than StEP due to the absence of pauses.

In Degenerate Oligonucleotide Gene Shuffling (DOGS) degenerate primers are used to control recombination between molecules; (Bergquist and Gibbs, Methods Mol.Biol 352: 191-204 (2007); Bergquis et al., Biomol.Eng 22:63-72 (2005); Gibbs et al., Gene 271 :13-20 (2001)) this can be used to control the tendency of other methods such as DNA shuffling to regenerate parental genes. This method can be combined with random mutagenesis (epPCR) of selected gene segments. This can be a good method to block the reformation of parental sequences. No endonucleases are needed. By adjusting input concentrations of segments made, one can bias towards a desired backbone. This method allows DNA shuffling from unrelated parents without restriction enzyme digests and allows a choice of random mutagenesis methods.

Incremental Truncation for the Creation of Hybrid Enzymes (ITCHY) creates a combinatorial library with 1 base pair deletions of a gene or gene fragment of interest. (Ostermeier et al., Proc Natl Acad Sd US.A 96:3562-3567 (1999); Ostermeier et al., Nat.Biotechnol 17:1205-1209 (1999)). Truncations are introduced in opposite direction on pieces of 2 different genes. These are ligated together and the fusions are cloned. This technique does not require homology between the 2 parental genes. When ITCHY is combined with DNA shuffling, the system is called SCRATCHY (see below). A major advantage of both is no need for homology between parental genes; for example, functional fusions between an E. coli and a human gene were created via ITCHY. When ITCHY libraries are made, all possible crossovers are captured.

Thio-Incremental Truncation for the Creation of Hybrid Enzymes (THIO-ITCHY) is almost the same as ITCHY except that phosphothioate dNTPs are used to generate truncations. (Lutz, S., M. Ostermeier, and S. J. Benkovic, 2001, Rapid generation of incremental truncation libraries for protein engineering using alpha-phosphothioate nucleotides. Nucleic Acids Res 29:E16.) Relative to ITCHY, THIO-ITCHY can be easier to optimize, provide more reproducibility, and adjustability.

SCRATCHY - ITCHY combined with DNA shuffling is a combination of DNA shuffling and ITCHY; therefore, allowing multiple crossovers. (Lutz et al., Proc Natl Acad Sci U S.A 98:11248-11253 (2001)). SCRATCHY combines the best features of ITCHY and DNA shuffling. Computational predictions can be used in optimization. SCRATCHY is more effective than DNA shuffling when sequence identity is below 80%.

In Random Drift Mutagenesis (RNDM) mutations made via epPCR followed by screening/selection for those retaining usable activity. (Bergquist et al., Biomol.Eng 22:63-72 (2005)). Then, these are used in DOGS to generate recombinants with fusions between multiple active mutants or between active mutants and some other desirable parent. Designed to promote isolation of neutral mutations; its purpose is to screen for retained catalytic activity whether or not this activity is higher or lower than in the original gene. RNDM is usable in high throughput assays when screening is capable of detecting activity above background. RNDM has been used as a front end to DOGS in generating diversity. The technique imposes a requirement for activity prior to shuffling or other subsequent steps; neutral drift libraries are indicated to result in higher/quicker improvements in activity from smaller libraries. Though published using epPCR, this could be applied to other large-scale mutagenesis methods.

Sequence Saturation Mutagenesis (SeSaM) is a random mutagenesis method that: 1) generates pool of random length fragments using random incorporation of a phosphothioate nucleotide and cleavage; this pool is used as a template to 2) extend in the presence of "universal" bases such as inosine; 3) replication of a inosine-containing complement gives random base incorporation and, consequently, mutagenesis. (Wong et al., PCR. Biotechnol J 3 :74-82 (2008); Wong et al., Nucleic Acids Res 32:e26 (2004); and Wong et al., Anal.Biochem. 341 :187-189 (2005)). Using this technique it can be possible to generate a large library of mutants within 2 -3 days using simple methods. This is very non-directed compared to mutational bias of DNA polymerases. Differences in this approach makes this technique complementary (or alternative) to epPCR.

In Synthetic Shuffling, overlapping oligonucleotides are designed to encode "all genetic diversity in targets" and allow a very high diversity for the shuffled progeny. (Ness et al., Nat.Biotechnol 20: 1251-1255 (2002)) In this technique, one can design the fragments to be shuffled. This aids in increaseing the resulting diversity of the progeny. One can design sequence/codon biases to make more distantly related sequences recombine at rates approaching more closely related sequences and it doesn't require possessing the template genes physically.

Nucleotide Exchange and Excision Technology NexT exploits a combination of dUTP incorporation followed by treatment with uracil DNA glycosylase and then piperidine to perform endpoint DNA fragmentation. (Muller et al., Nucleic Acids Res 33 :el 17 (2005)). The gene is reassembled using internal PCR primer extension with proofreading polymerase. The sizes for shuffling are directly controllable using varying dUPT::dTTP ratios. This is an end point reaction using simple methods for uracil incorporation and cleavage. One can use other nucleotide analogs such as 8-oxo-guanine with this method. Additionally, the technique works well with very short fragments (86 bp) and has a low error rate. Chemical cleavage of DNA means very few unshuffled clones.

In Sequence Homology-Independent Protein Recombination (SHIPREC) a linker is used to facilitate fusion between 2 distantly/unrelated genes; nuclease treatment is used to generate a range of chimeras between the two. Result is a single crossover library of these fusions. (Sieber et al., Nat.Biotechnol 19:456-460 (2001)). This produces a limited type of shuffling; mutagenesis is a separate process. This technique can create a library of chimeras with varying fractions of each of 2 unrelated parent genes. No homology is needed. SHIPREC was tested with a heme-binding domain of a bacterial CP450 fused to ^-terminal regions of a mammalian CP450; this produced mammalian activity in a more soluble enzyme.

In Gene Site Saturation Mutagenesis (GSSM) the starting materials are a supercoiled dsDNA plasmid with insert and 2 primers degenerate at the desired site for mutations. (Kretz et al., Methods Enzymol. 388:3-11 (2004)). Primers carry the mutation of interest and anneal to the same sequence on opposite strands of DNA; mutation in the middle of the primer and ~20 nucleotides of correct sequence flanking on each side. The sequence in the primer is NNN or NNK (coding) and MNN (noncoding) (N = all 4, K = G, T, M = A, C). After extension, Dpnl is used to digest dam-methylated DNA to eliminate the wild-type template. This technique explores all possible amino acid substitutions at a given locus (i.e., one codon). The technique facilitates the generation of all possible replacements at one site with no nonsense codons and equal or near-equal representation of most possible alleles. It does not require prior knowledge of structure, mechanism, or domains of the target enzyme. If followed by shuffling or Gene Reassembly, this technology creates a diverse library of recombinants containing all possible combinations of single-site up-mutations. The utility of this technology combination has been demonstrated for the successful evolution of over 50 different enzymes, and also for more than one property in a given enzyme.

Combinatorial Cassette Mutagenesis (CCM)involves the use of short oligonucleotide cassettes to replace limited regions with a large number of possible amino acid sequence alterations. (Reidhaar-Olson et al., Methods Enzymol. 208:564-586 (1991); and Reidhaar-Olson et al., Science 241 :53-57 (1988)). Simultaneous substitutions at 2 or 3 sites are possible using this technique. Additionally, the method tests a large multiplicity of possible sequence changes at a limited range of sites. It has been used to explore the information content of lambda repressor DNA-binding domain.

Combinatorial Multiple Cassette Mutagenesis (CMCM)is essentially similar to CCM except it is employed as part of a larger program: 1) Use of epPCR at high mutation rate to 2) ID hot spots and hot regions and then 3) extension by CMCM to cover a defined region of protein sequence space. (Reetz et al., Angew.Chem.Int.Ed Engl. 40:3589-3591 (2001)). As with CCM, this method can test virtually all possible alterations over a target region. If used along with methods to create random mutations and shuffled genes, it provides an excellent means of generating diverse, shuffled proteins. This approach was successful in increasing, by 51 -fold, the enantioselectivity of an enzyme.

In the Mutator Strains technique conditional ts mutator plasmids allow increases of 20- to 4000- X in random and natural mutation frequency during selection and to block accumulation of deleterious mutations when selection is not required. (Selifonova et al, Appl Environ Microbiol 67:3645-3649 (2001)). This technology is based on a plasmid-derived mutD5 gene, which encodes a mutant subunit of DNA polymerase III. This subunit binds to endogenous DNA polymerase III and compromises the proofreading ability of polymerase III in any of the strain that harbors the plasmid. A broad-spectrum of base substitutions and frameshift mutations occur. In order for effective use, the mutator plasmid should be removed once the desired phenotype is achieved; this is accomplished through a temperature sensitive origin of replication, which allows plasmid curing at 41⁰C. It should be noted that mutator strains have been explored for quite some time (e.g., see Winter and coworkers, 1996, J. MoL Biol. 260, 359- 3680. In this technique very high spontaneous mutation rates are observed. The conditional property minimizes non-desired background mutations. This technology could be combined with adaptive evolution to enhance mutagenesis rates and more rapidly achieve desired phenotypes.

"Look-Through Mutagenesis (LTM) is a multidimensional mutagenesis method that assesses and optimizes combinatorial mutations of selected amino acids." (Rajpal et al., Proc Natl Acad Sci US.A 102:8466-8471 (2005)). Rather than saturating each site with all possible amino acid changes, a set of 9 is chosen to cover the range of amino acid R-group chemistry. Fewer changes per site allows multiple sites to be subjected to this type of mutagenesis. A >800-fold increase in binding affinity for an antibody from low nanomolar to picomolar has been achieved through this method. This is a rational approach to minimize the number of random combinations and should increase the ability to find improved traits by greatly decreasing the numbers of clones to be screened. This has been applied to antibody engineering, specifically to increase the binding affinity and/or reduce dissociation. The technique can be combined with either screens or selections.

Gene Reassembly is a DNA shuffling method that can be applied to multiple genes at one time or to creating a large library of chimeras (multiple mutations) of a single gene, (on the worldwide web at www.verenium.com/Pages/Technology/EnzymeTech/TechEnzyTGR.html) Typically this technology is used in combination with ultra-high-throughput screening to query the represented sequence space for desired improvements. This technique allows multiple gene recombination independent of homology. The exact number and position of cross-over events can be pre-determined using fragments designed via bioinformatic analysis. This technology leads to a very high level of diversity with virtually no parental gene reformation and a low level of inactive genes. Combined with GSSM, a large range of mutations can be tested for improved activity. The method allows "blending" and "fine tuning" of DNA shuffling, e.g. codon usage can be optimized.

In Silico Protein Design Automation PDA is an optimization algorithm that anchors the structurally defined protein backbone possessing a particular fold, and searches sequence space for amino acid substitutions that can stabilize the fold and overall protein energetics. (Hayes et al., Proc Natl Acad Sci U S.A 99:15926-15931 (2002)). This technology allows in silico structure-based entropy predictions in order to search for structural tolerance toward protein amino acid variations. Statistical mechanics is applied to calculate coupling interactions at each position - structural tolerance toward amino acid substitution is a measure of coupling. Ultimately, this technology is designed to yield desired modifications of protein properties while maintaining the integrity of structural characteristics. The method computationally assesses and allows filtering of a very large number of possible sequence variants (10⁵⁰). Choice of sequence variants to test is related to predictions based on most favorable thermodynamics and ostensibly only stability or properties that are linked to stability can be effectively addressed with this technology. The method has been successfully used in some therapeutic proteins, especially in engineering immunoglobulins. In silico predictions avoid testing extraordinarily large numbers of potential variants. Predictions based on existing three-dimensional structures are more likely to succeed than predictions based on hypothetical structures. This technology can readily predict and allow targeted screening of multiple simultaneous mutations, something not possible with purely experimental technologies due to exponential increases in numbers.

Iterative Saturation Mutagenesis (ISM)involves 1) Use knowledge of structure/function to choose a likely site for enzyme improvement. 2) Saturation mutagenesis at chosen site using Stratagene QuikChange (or other suitable means). 3) Screen/select for desired properties. 4) With improved clone(s), start over at another site and continue repeating. (Reetz et al., Nat.Protoc. 2:891-903 (2007); and Reetz et al., Angew.Chem.Int.Ed Engl. 45:7745-7751 (2006)). This is a proven methodology assures all possible replacements at a given position are made for screening/selection. Any of the aforementioned methods for mutagenesis can be used alone or in any combination. Additionally, any one or combination of the directed evolution methods can be used in conjunction with adaptive evolution techniques.

To generate better producers, metabolic modeling can be utilized to optimize growth conditions. Modeling can also be used to design gene knockouts that additionally optimize utilization of the pathway (see, for example, U.S. patent publications US 2002/0012939, US 2003/0224363, US 2004/0029149, US 2004/0072723, US 2003/0059792, US 2002/0168654 and US 2004/0009466, and U.S. Patent No. 7,127,379). Modeling analysis allows reliable predictions of the effects on cell growth of shifting the metabolism towards more efficient production of isopropanol, n- butanol, or isobutanol.

One computational method for identifying and designing metabolic alterations favoring biosynthesis of a desired product is the OptKnock computational framework (Burgard et al., Biotechnol. Bioeng. 84:647-657 (2003)). OptKnock is a metabolic modeling and simulation program that suggests gene deletion strategies that result in genetically stable microorganisms which overproduce the target product. Specifically, the framework examines the complete metabolic and/or biochemical network of a microorganism in order to suggest genetic manipulations that force the desired biochemical to become an obligatory byproduct of cell growth. By coupling biochemical production with cell growth through strategically placed gene deletions or other functional gene disruption, the growth selection pressures imposed on the engineered strains after long periods of time in a bioreactor lead to improvements in performance as a result of the compulsory growth-coupled biochemical production. Lastly, when gene deletions are constructed there is a negligible possibility of the designed strains reverting to their wild-type states because the genes selected by OptKnock are to be completely removed from the genome. Therefore, this computational methodology can be used to either identify alternative pathways that lead to biosynthesis of a desired product or used in connection with the no n- naturally occurring microbial organisms for further optimization of biosynthesis of a desired product.

Briefly, OptKnock is a term used herein to refer to a computational method and system for modeling cellular metabolism. The OptKnock program relates to a framework of models and methods that incorporate particular constraints into flux balance analysis (FBA) models. These constraints include, for example, qualitative kinetic information, qualitative regulatory information, and/or DNA microarray experimental data. OptKnock also computes solutions to various metabolic problems by, for example, tightening the flux boundaries derived through flux balance models and subsequently probing the performance limits of metabolic networks in the presence of gene additions or deletions. OptKnock computational framework allows the construction of model formulations that enable an effective query of the performance limits of metabolic networks and provides methods for solving the resulting mixed-integer linear programming problems. The metabolic modeling and simulation methods referred to herein as OptKnock are described in, for example, U.S. 2002/0168654, WO 2002/055995, and U.S. 2009/0047719.

Another computational method for identifying and designing metabolic alterations favoring biosynthetic production of a product is a metabolic modeling and simulation system termed SimPheny®. This computational method and system is described in, for example, U.S.

2003/0233218, filed June 14, 2002, and in WO/2003/106998. SimPheny® is a computational system that can be used to produce a network model in silico and to simulate the flux of mass, energy or charge through the chemical reactions of a biological system to define a solution space that contains any and all possible functionalities of the chemical reactions in the system, thereby determining a range of allowed activities for the biological system. This approach is referred to as constraints-based modeling because the solution space is defined by constraints such as the known stoichiometry of the included reactions as well as reaction thermodynamic and capacity constraints associated with maximum fluxes through reactions. The space defined by these constraints can be interrogated to determine the phenotypic capabilities and behavior of the biological system or of its biochemical components.

These computational approaches are consistent with biological realities because biological systems are flexible and can reach the same result in many different ways. Biological systems are designed through evolutionary mechanisms that have been restricted by fundamental constraints that all living systems must face. Therefore, constraints-based modeling strategy embraces these general realities. Further, the ability to continuously impose further restrictions on a network model via the tightening of constraints results in a reduction in the size of the solution space, thereby enhancing the precision with which physiological performance or phenotype can be predicted.

Given the teachings and guidance provided herein, those skilled in the art will be able to apply various computational frameworks for metabolic modeling and simulation to design and implement biosynthesis of a desired compound in host microbial organisms. Such metabolic modeling and simulation methods include, for example, the computational systems exemplified above as SimPheny® and OptKnock. For illustration of the invention, some methods are described herein with reference to the OptKnock computation framework for modeling and simulation. Those skilled in the art will know how to apply the identification, design and implementation of the metabolic alterations using OptKnock to any of such other metabolic modeling and simulation computational frameworks and methods well known in the art.

The methods described above will provide one set of metabolic reactions to disrupt. Elimination of each reaction within the set or metabolic modification can result in a desired product as an obligatory product during the growth phase of the organism. Because the reactions are known, a solution to the bilevel OptKnock problem also will provide the associated gene or genes encoding one or more enzymes that catalyze each reaction within the set of reactions. Identification of a set of reactions and their corresponding genes encoding the enzymes participating in each reaction is generally an automated process, accomplished through correlation of the reactions with a reaction database having a relationship between enzymes and encoding genes.

Once identified, the set of reactions that are to be disrupted in order to achieve production of a desired product are implemented in the target cell or organism by functional disruption of at least one gene encoding each metabolic reaction within the set. One particularly useful means to achieve functional disruption of the reaction set is by deletion of each encoding gene. However, in some instances, it can be beneficial to disrupt the reaction by other genetic aberrations including, for example, mutation, deletion of regulatory regions such as promoters or cis binding sites for regulatory factors, or by truncation of the coding sequence at any of a number of locations. These latter aberrations, resulting in less than total deletion of the gene set can be useful, for example, when rapid assessments of the coupling of a product are desired or when genetic reversion is less likely to occur.

To identify additional productive solutions to the above described bilevel OptKnock problem which lead to further sets of reactions to disrupt or metabolic modifications that can result in the biosynthesis, including growth-coupled biosynthesis of a desired product, an optimization method, termed integer cuts, can be implemented. This method proceeds by iteratively solving the OptKnock problem exemplified above with the incorporation of an additional constraint referred to as an integer cut at each iteration. Integer cut constraints effectively prevent the solution procedure from choosing the exact same set of reactions identified in any previous iteration that obligatorily couples product biosynthesis to growth. For example, if a previously identified growth-coupled metabolic modification specifies reactions 1, 2, and 3 for disruption, then the following constraint prevents the same reactions from being simultaneously considered in subsequent solutions. The integer cut method is well known in the art and can be found described in, for example, Burgard et al., Biotechnol. Prog. 17:791-797 (2001). As with all methods described herein with reference to their use in combination with the OptKnock computational framework for metabolic modeling and simulation, the integer cut method of reducing redundancy in iterative computational analysis also can be applied with other computational frameworks well known in the art including, for example, SimPheny®.

The methods exemplified herein allow the construction of cells and organisms that biosynthetically produce a desired product, including the obligatory coupling of production of a target biochemical product to growth of the cell or organism engineered to harbor the identified genetic alterations. Therefore, the computational methods described herein allow the identification and implementation of metabolic modifications that are identified by an in silico method selected from OptKnock or SimPheny®. The set of metabolic modifications can include, for example, addition of one or more biosynthetic pathway enzymes and/or functional disruption of one or more metabolic reactions including, for example, disruption by gene deletion.

As discussed above, the OptKnock methodology was developed on the premise that mutant microbial networks can be evolved towards their computationally predicted maximum-growth phenotypes when subjected to long periods of growth selection. In other words, the approach leverages an organism's ability to self-optimize under selective pressures. The OptKnock framework allows for the exhaustive enumeration of gene deletion combinations that force a coupling between biochemical production and cell growth based on network stoichiometry. The identification of optimal gene/reaction knockouts requires the solution of a bilevel optimization problem that chooses the set of active reactions such that an optimal growth solution for the resulting network overproduces the biochemical of interest (Burgard et al., Biotechnol. Bioeng. 84:647-657 (2003)).

An in silico stoichiometric model of E. coli metabolism can be employed to identify essential genes for metabolic pathways as exemplified previously and described in, for example, U.S. patent publications US 2002/0012939, US 2003/0224363, US 2004/0029149, US 2004/0072723, US 2003/0059792, US 2002/0168654 and US 2004/0009466, and in U.S. Patent No. 7,127,379. As disclosed herein, the OptKnock mathematical framework can be applied to pinpoint gene deletions leading to the growth-coupled production of a desired product. Further, the solution of the bilevel OptKnock problem provides only one set of deletions. To enumerate all meaningful solutions, that is, all sets of knockouts leading to growth-coupled production formation, an optimization technique, termed integer cuts, can be implemented. This entails iteratively solving the OptKnock problem with the incorporation of an additional constraint referred to as an integer cut at each iteration, as discussed above.

It is understood that modifications which do not substantially affect the activity of the various embodiments of this invention are also included within the definition of the invention provided herein. Accordingly, the following examples are intended to illustrate but not limit the present invention.

EXAMPLE I

Preparation of an Isopropanol Producing Microbial Organism Having a Pathway for Converting 4-Hydroxybutyryl-CoA to Isopropanol

This example describes the generation of a microbial organism capable of producing isopropanol from 4-hydroxybutyryl-CoA.

Escherichia coli is used as a target organism to engineer the isopropanol pathway shown in Figure 1 that starts from 4-hydroxybutyryl-CoA. E. coli provides a good host for generating a non-naturally occurring microorganism capable of producing isopropanol. E. coli is amenable to genetic manipulation and is known to be capable of producing various products, like ethanol, acetic acid, formic acid, lactic acid, and succinic acid, effectively under anaerobic or microaerobic conditions.

To generate an is. coli strain engineered to produce 3 -hydro xyisobutyric acid, nucleic acids encoding the enzymes utilized in the pathway are expressed in E. coli using well known molecular biology techniques (see, for example, Sambrook, supra, 2001; Ausubel supra, 1999). In particular, the sucD (YP_001396394), 4hbd (YP_001396393), bukl (Q45829), anάptb (NP_349676) genes encoding succinic semialdehyde dehydrogenase (CoA-dependent), A- hydroxybutyrate dehydrogenase, 4-hydroxybutyrate kinase, and phosphotransbutyrylase activities, respectively, are cloned into an expression vector or integrated into the chromosome as described in Burk et al. (U.S. publication 2009/0075351). In addition, the abfD (P55792) and crtl (NP_349318.1) genes encoding 4-hydroxybutyryl-CoA dehydratase, vinylacetyl-CoA Δ- isomerase, and enoyl-CoA hydratase activities, respectively, are cloned into the pZE13 vector (Expressys, Ruelzheim, Germany) under the PAl/lacO promoter. Next, the hbd (NP_349314.1) and atoAD (P76459.1, P76458.1) encoding 3-hydroxybutyryl-CoA dehydrogenase and acetyl- CoA:acetoacetate-CoA transferase activities, respectively, are cloned into the pZS23 vector (Expressys, Ruelzheim, Germany) under the PAl/lacO promoter. Then, the adc (NP_149328.1) and adh (AAA23199.2) genes encoding acetoacetate decarboxylase and acetone reductase activities are cloned into the pZS13 vector (Expressys, Ruelzheim, Germany) under the PAl/lacO promoter. pZS23 is obtained by replacing the ampicillin resistance module of the pZS13 vector (Expressys, Ruelzheim, Germany) with a kanamycin resistance module by well- known molecular biology techniques. The three sets of plasmids are transformed into a 4- hydroxybutyryl-CoA producing strain of E. coli to express the proteins and enzymes required for isopropanol synthesis from 4-hydroxybutyryl-CoA.

The resulting genetically engineered organism is cultured in glucose-containing medium following procedures well known in the art (see, for example, Sambrook et al., supra, 2001). Cobalamin is also supplied to the medium to ensure activity of the mutase enzyme unless the host strain of E. coli is engineered to synthesize cobalamin de novo (see, for example, Raux et al., J. Bacteriol. 178:753-767 (1996)). The expression of the isopropanol synthesis genes is corroborated using methods well known in the art for determining polypeptide expression or enzymatic activity, including for example, Northern blots, PCR amplification of mRNA, immunoblotting, and the like. Enzymatic activities of the expressed enzymes are confirmed using assays specific for the individual activities. The ability of the engineered E. coli strain to produce isopropanol is confirmed using HPLC, gas chromatography-mass spectrometry (GCMS) and/or liquid chromatography-mass spectrometry (LCMS).

Microbial strains engineered to have a functional isopropanol synthesis pathway are further augmented by optimization for efficient utilization of the pathway. Briefly, the engineered strain is assessed to determine whether any of the exogenous genes are expressed at a rate limiting level. Expression is increased for any enzymes expressed at low levels that can limit the flux through the pathway by, for example, introduction of additional gene copy numbers.

To generate better producers, metabolic modeling is utilized to optimize growth conditions. Modeling is also used to design gene knockouts that additionally optimize utilization of the pathway (see, for example, U.S. patent publications US 2002/0012939, US 2003/0224363, US 2004/0029149, US 2004/0072723, US 2003/0059792, US 2002/0168654 and US 2004/0009466, and U.S. Patent No. 7,127,379). Modeling analysis allows reliable predictions of the effects on cell growth of shifting the metabolism towards more efficient production of isopropanol. One modeling method is the bilevel optimization approach, OptKnock (Burgard et al., Biotechnol. Bioengineer. 84:647-657 (2003)), which is applied to select gene knockouts that collectively result in better production of isopropanol. Adaptive evolution also can be used to generate better producers of, for example, the 4-hydroxybutyryl-CoA intermediate of the isopropanol product. Adaptive evolution is performed to improve both growth and production characteristics (Fong and Palsson, Nat. Genet. 36:1056-1058 (2004); Alper et al., Science 314: 1565-1568 (2006)). Based on the results, subsequent rounds of modeling, genetic engineering and adaptive evolution can be applied to the isopropanol producer to further increase production.

For large-scale production of isopropanol, the above organism is cultured in a fermenter using a medium known in the art to support growth of the organism under anaerobic conditions. Fermentations are performed in either a batch, fed-batch or continuous manner. Anaerobic conditions are maintained by first sparging the medium with nitrogen and then sealing the culture vessel, for example, flasks can be sealed with a septum and crimp-cap. Microaerobic conditions also can be utilized by providing a small hole in the septum for limited aeration. The pH of the medium is maintained at a pH of around 7 by addition of an acid, such as H₂SO₄. The growth rate is determined by measuring optical density using a spectrophotometer (600 nm) and the glucose uptake rate by monitoring carbon source depletion over time. Byproducts such as undesirable alcohols, organic acids, and residual glucose can be quantified by HPLC (Shimadzu, Columbia MD), for example, using an Aminex® series of HPLC columns (for example, HPX-87 series) (BioRad, Hercules CA), using a refractive index detector for glucose and alcohols, and a UV detector for organic acids (Lin et al., Biotechnol. Bioeng. 775-779 (2005)).

EXAMPLE II

Preparation of a /t-Butanol Producing Microbial Organism Having a Pathway for Converting 4-Hydroxybutyryl-CoA to w-Butanol

This example describes the generation of a microbial organism capable of producing w-butanol from 4-hydroxybutyryl-CoA.

Escherichia coli is used as a target organism to engineer the butanol pathway shown in Figure 1 that starts from 4-hydroxybutyryl-CoA. E. coli provides a good host for generating a non- naturally occurring microorganism capable of producing butanol. E. coli is amenable to genetic manipulation and is known to be capable of producing various products, like ethanol, acetic acid, formic acid, lactic acid, and succinic acid, effectively under anaerobic or microaerobic conditions.

To generate an E. coli strain engineered to produce 3 -hydro xyisobutyric acid, nucleic acids encoding the enzymes utilized in the pathway are expressed in E. coli using well known molecular biology techniques (see, for example, Sambrook, supra, 2001; Ausubel supra, 1999). In particular, the sucD (YP_001396394), 4hbd (YP_001396393), bukl (Q45829), znάptb (NP_349676) genes encoding succinic semialdehyde dehydrogenase (CoA-dependent), 4- hydroxybutyrate dehydrogenase, 4-hydroxybutyrate kinase, and phosphotransbutyrylase activities, respectively, are cloned into an expression vector or integrated into the chromosome as described in Burk et al. (U.S. publication 2009/0075351). In addition, the abfD (P55792) gene encoding 4-hydroxybutyryl-CoA dehydratase and vinylacetyl-CoA Δ-isomerase activities is cloned into the pZE13 vector (Expressys, Ruelzheim, Germany) under the PAl/lacO promoter. Next, the bed (NP_349317.1) and etfAB (NP_349315.1, NPJ49316.1) genes encoding crotonyl-CoA reductase activity are cloned into the pZS23 vector (Expressys, Ruelzheim, Germany) under the PAl/lacO promoter. Then, the aid (AAT66436) and adhl (AAR91477.1) genes encoding butyryl-CoA reductase (aldehyde forming) and butyraldehyde reductase activities are cloned into the pZS13 vector (Expressys, Ruelzheim, Germany) under the PAl/lacO promoter. pZS23 is obtained by replacing the ampicillin resistance module of the pZS13 vector (Expressys, Ruelzheim, Germany) with a kanamycin resistance module by well- known molecular biology techniques. The three sets of plasmids are transformed into a 4- hydroxybutyryl-CoA producing strain of is. coli to express the proteins and enzymes required for butanol synthesis from 4-hydroxybutyryl-CoA.

The resulting genetically engineered organism is cultured in glucose-containing medium following procedures well known in the art (see, for example, Sambrook et al., supra, 2001). Cobalamin is also supplied to the medium to ensure activity of the mutase enzyme unless the host strain of is. coli is engineered to synthesize cobalamin de novo (see, for example, Raux et al., J. Bacteriol. 178:753-767 (1996)). The expression of the butanol synthesis genes is corroborated using methods well known in the art for determining polypeptide expression or enzymatic activity, including for example, Northern blots, PCR amplification of mRNA, immunoblotting, and the like. Enzymatic activities of the expressed enzymes are confirmed using assays specific for the individual activities. The ability of the engineered E. coli strain to produce butanol is confirmed using HPLC, gas chromatography-mass spectrometry (GCMS) and/or liquid chromatography-mass spectrometry (LCMS).

Microbial strains engineered to have a functional butanol synthesis pathway are further augmented by optimization for efficient utilization of the pathway. Briefly, the engineered strain is assessed to determine whether any of the exogenous genes are expressed at a rate limiting level. Expression is increased for any enzymes expressed at low levels that can limit the flux through the pathway by, for example, introduction of additional gene copy numbers. To generate better producers, metabolic modeling is utilized to optimize growth conditions. Modeling is also used to design gene knockouts that additionally optimize utilization of the pathway (see, for example, U.S. patent publications US 2002/0012939, US 2003/0224363, US 2004/0029149, US 2004/0072723, US 2003/0059792, US 2002/0168654 and US 2004/0009466, and U.S. Patent No. 7,127,379). Modeling analysis allows reliable predictions of the effects on cell growth of shifting the metabolism towards more efficient production of butanol. One modeling method is the bilevel optimization approach, OptKnock (Burgard et al., Biotechnol. Bioengineer. 84:647-657 (2003)), which is applied to select gene knockouts that collectively result in better production of butanol. Adaptive evolution also can be used to generate better producers of, for example, the 4-hydroxybutyryl-CoA intermediate of the butanol product.

Adaptive evolution is performed to improve both growth and production characteristics (Fong and Palsson, Nat. Genet. 36:1056-1058 (2004); Alper et al., Science 314:1565-1568 (2006)). Based on the results, subsequent rounds of modeling, genetic engineering and adaptive evolution can be applied to the butanol producer to further increase production.

For large-scale production of butanol, the above organism is cultured in a fermenter using a medium known in the art to support growth of the organism under anaerobic conditions. Fermentations are performed in either a batch, fed-batch or continuous manner. Anaerobic conditions are maintained by first sparging the medium with nitrogen and then sealing the culture vessel, for example, flasks can be sealed with a septum and crimp-cap. Microaerobic conditions also can be utilized by providing a small hole in the septum for limited aeration. The pH of the medium is maintained at a pH of around 7 by addition of an acid, such as H₂SO₄. The growth rate is determined by measuring optical density using a spectrophotometer (600 nm) and the glucose uptake rate by monitoring carbon source depletion over time. Byproducts such as undesirable alcohols, organic acids, and residual glucose can be quantified by HPLC (Shimadzu, Columbia MD), for example, using an Aminex® series of HPLC columns (for example, HPX-87 series) (BioRad, Hercules CA), using a refractive index detector for glucose and alcohols, and a UV detector for organic acids (Lin et al., Biotechnol. Bioeng. 775-779 (2005)).

EXAMPLE III

Preparation of an Isobutanol Producing Microbial Organism Having a Pathway for Converting 4-Hydroxybutyryl-CoA to Isobutanol

This example describes the generation of a microbial organism capable of producing isobutanol from 4-hydroxybutyryl-CoA. Escherichia coli is used as a target organism to engineer the isobutanol pathway shown in Figure 1 that starts from 4-hydroxybutyryl-CoA. E. coli provides a good host for generating a non- naturally occurring microorganism capable of producing isobutanol. E. coli is amenable to genetic manipulation and is known to be capable of producing various products, like ethanol, acetic acid, formic acid, lactic acid, and succinic acid, effectively under anaerobic or microaerobic conditions.

To generate an E. coli strain engineered to produce 3 -hydro xyisobutyric acid, nucleic acids encoding the enzymes utilized in the pathway are expressed in E. coli using well known molecular biology techniques (see, for example, Sambrook, supra, 2001; Ausubel supra, 1999). In particular, the sucD (YP_001396394), 4hbd (YP_001396393), bukl (Q45829), anάptb (NP_349676) genes encoding succinic semialdehyde dehydrogenase (CoA-dependent), A- hydroxybutyrate dehydrogenase, 4-hydroxybutyrate kinase, and phosphotransbutyrylase activities, respectively, are cloned into an expression vector or integrated into the chromosome as described in Burk et al. (U.S. publication 2009/0075351). In addition, the abfD (P55792), icm (AAC08713.1), and icmB (CAB59633.1) genes encoding 4-hydroxybutyryl-CoA dehydratase, vinylacetyl-CoA Δ-isomerase, and isobutyryl-CoA mutase activities are cloned into the pZE13 vector (Expressys, Ruelzheim, Germany) under the PAl/lacO promoter. Next, the bed (NP_349317.1) and etfAB (NP_349315.1, NP_349316.1) genes encoding crotonyl-CoA reductase activity are cloned into the pZS23 vector (Expressys, Ruelzheim, Germany) under the PAl/lacO promoter. Then, the adhE (AAV66076.1) gene encoding isobutyryl-CoA reductase (alcohol forming) activity is cloned into the pZS13 vector (Expressys, Ruelzheim, Germany) under the PAl/lacO promoter. pZS23 is obtained by replacing the ampicillin resistance module of the pZS13 vector (Expressys, Ruelzheim, Germany) with a kanamycin resistance module by well-known molecular biology techniques. The three sets of plasmids are transformed into a A- hydroxybutyryl-CoA producing strain of is. coli to express the proteins and enzymes required for isobutanol synthesis from 4-hydroxybutyryl-CoA.

The resulting genetically engineered organism is cultured in glucose-containing medium following procedures well known in the art (see, for example, Sambrook et al., supra, 2001). Cobalamin is also supplied to the medium to ensure activity of the mutase enzyme unless the host strain of E. coli is engineered to synthesize cobalamin de novo (see, for example, Raux et al., J. Bacteriol. 178:753-767 (1996)). The expression of the isobutanol synthesis genes is corroborated using methods well known in the art for determining polypeptide expression or enzymatic activity, including for example, Northern blots, PCR amplification of mRNA, immunoblotting, and the like. Enzymatic activities of the expressed enzymes are confirmed using assays specific for the individual activities. The ability of the engineered E. coli strain to produce isobutanol is confirmed using HPLC, gas chromatography-mass spectrometry (GCMS) and/or liquid chromatography-mass spectrometry (LCMS).

Microbial strains engineered to have a functional isobutanol synthesis pathway are further augmented by optimization for efficient utilization of the pathway. Briefly, the engineered strain is assessed to determine whether any of the exogenous genes are expressed at a rate limiting level. Expression is increased for any enzymes expressed at low levels that can limit the flux through the pathway by, for example, introduction of additional gene copy numbers.

To generate better producers, metabolic modeling is utilized to optimize growth conditions. Modeling is also used to design gene knockouts that additionally optimize utilization of the pathway (see, for example, U.S. patent publications US 2002/0012939, US 2003/0224363, US 2004/0029149, US 2004/0072723, US 2003/0059792, US 2002/0168654 and US 2004/0009466, and U.S. Patent No. 7,127,379). Modeling analysis allows reliable predictions of the effects on cell growth of shifting the metabolism towards more efficient production of isobutanol. One modeling method is the bilevel optimization approach, OptKnock (Burgard et al., Biotechnol. Bioengineer. 84:647-657 (2003)), which is applied to select gene knockouts that collectively result in better production of isobutanol. Adaptive evolution also can be used to generate better producers of, for example, the 4-hydroxybutyryl-CoA intermediate of the isobutanol product. Adaptive evolution is performed to improve both growth and production characteristics (Fong and Palsson, Nat. Genet. 36:1056-1058 (2004); Alper et al., Science 314:1565-1568 (2006)). Based on the results, subsequent rounds of modeling, genetic engineering and adaptive evolution can be applied to the isobutanol producer to further increase production.

For large-scale production of isobutanol, the above organism is cultured in a fermenter using a medium known in the art to support growth of the organism under anaerobic conditions. Fermentations are performed in either a batch, fed-batch or continuous manner. Anaerobic conditions are maintained by first sparging the medium with nitrogen and then sealing the culture vessel, for example, flasks can be sealed with a septum and crimp-cap. Microaerobic conditions also can be utilized by providing a small hole in the septum for limited aeration. The pH of the medium is maintained at a pH of around 7 by addition of an acid, such as H2SO4. The growth rate is determined by measuring optical density using a spectrophotometer (600 nm) and the glucose uptake rate by monitoring carbon source depletion over time. Byproducts such as undesirable alcohols, organic acids, and residual glucose can be quantified by HPLC (Shimadzu, Columbia MD), for example, using an Aminex® series of HPLC columns (for example, HPX-87 series) (BioRad, Hercules CA), using a refractive index detector for glucose and alcohols, and a UV detector for organic acids (Lin et al., Biotechnol. Bioeng. 775-779 (2005)).

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Throughout this application, references are made to various patents, published pending applications, and non-patent literature references. All such references are incorporated herein by reference in their entirety.

Although the invention has been described with reference to the disclosed embodiments, those skilled in the art will readily appreciate that the specific examples and studies detailed above are only illustrative of the invention. It should be understood that various modifications can be made without departing from the spirit of the invention. Accordingly, the invention is limited only by the following claims.

Claims

What is claimed is:

1. A non-naturally occurring microbial organism, comprising a microbial organism having an isopropanol pathway comprising at least one exogenous nucleic acid encoding an isopropanol pathway enzyme expressed in a sufficient amount to produce isopropanol, said isopropanol pathway comprising an enzyme selected from the group consisting of a 4-hydroxybutyryl-CoA dehydratase, a crotonase, a 3-hydroxybutyryl-CoA dehydrogenase, an acetoacetyl-CoA synthetase, an acetyl-CoA:acetoacetate-CoA transferase, an acetoacetyl-CoA hydrolase, an acetoacetate decarboxylase, and an acetone reductase.

2. The non-naturally occurring microbial organism of claim 1, wherein said microbial organism comprises two exogenous nucleic acids, each encoding an isopropanol pathway enzyme.

3. The non-naturally occurring microbial organism of claim 1, wherein said microbial organism comprises three exogenous nucleic acids, each encoding an isopropanol pathway enzyme.

4. The non-naturally occurring microbial organism of claim 1, wherein said microbial organism comprises four exogenous nucleic acids, each encoding an isopropanol pathway enzyme.

5. The non-naturally occurring microbial organism of claim 1, wherein said microbial organism comprises five exogenous nucleic acids, each encoding an isopropanol pathway enzyme.

6. The non-naturally occurring microbial organism of claim 1, wherein said microbial organism comprises six exogenous nucleic acids, each encoding an isopropanol pathway enzyme.

7. The non-naturally occurring microbial organism of claim 1, wherein said microbial organism comprises seven exogenous nucleic acids, each encoding an isopropanol pathway enzyme.

8. The non-naturally occurring microbial organism of claim 1, wherein said microbial organism comprises eight exogenous nucleic acids, each encoding an isopropanol pathway enzyme.

9. The non-naturally occurring microbial organism of claim 1, wherein said at least one exogenous nucleic acid is a heterologous nucleic acid.

10. The non-naturally occurring microbial organism of claim 1, wherein said non-naturally occurring microbial organism is in a substantially anaerobic culture medium.

11. The non-naturally occurring microbial organism of claim 1, wherein said A- hydroxybutyryl-CoA dehydratase is encoded by one or more genes selected from the group consisting oifumA,fumB,fumC,fumH,fuml, MmcB, MmcC, hmd, BACCAP _02294 , ANACOL_02527, NtherDRAFT_2368, dmdA, dmdB, crt, crtl, paaA,paaB,phaA,phaB, maoC, paaF,paaG, abfD, and Msed_l 220.

12. The non-naturally occurring microbial organism of claim 11 , wherein said A- hydroxybutyryl-CoA dehydratase is encoded by abfD.

13. The non-naturally occurring microbial organism of claim 1, wherein said crotonase is encoded by one or more genes selected from the group consisting oifumA,fumB,fumC,fumH, fuml, MmcB, MmcC, hmd, BACCAP _02294, ANACOL_02527, NtherDRAFT_2368, dmdA, dmdB, crt, crtl,paaA, paaB, phaA, phaB, maoC, paaF, paaG, abfD, and Msed_1220.

14. The non-naturally occurring microbial organism of claim 13, wherein said crotonase is encoded by one or more genes selected from the group consisting of crt, crtl,paaA,paaB, phaA,phaB, maoC,paaF, and paaG.

15. The non-naturally occurring microbial organism of claim 1, wherein said 3- hydroxybutyryl-CoA dehydrogenase is encoded by one or more genes selected from the group consisting oithrA, akthr2, homo, homl, hom2,fadB,fadJ, Hbd2, Hbdl, hbd, HSD17B10,phbB, phaB, Msed_1423, Msed_0399, Msed_0389, Msed_1993, adh, adhA, adh-A, mdh, MhA, Uh, and bdh.

16. The non-naturally occurring microbial organism of claim 15, wherein said 3- hydroxybutyryl-CoA dehydrogenase is encoded by one or more genes selected from the group consisting of hbd, Hbd2, Hbdl, Msed_1423, Msed_0399, Msed_0389, Msed_1993,fadB, and fadJ.

17. The no n- naturally occurring microbial organism of claim 1, wherein said acetoacetyl- CoA synthetase is encoded by one or more genes selected from the group consisting of sucC, sucD, AACS, AF1211, scs, and PAE3250.

18. The non-naturally occurring microbial organism of claim 17, wherein said acetoacetyl- CoA synthetase is encoded by one or more genes selected from the group consisting of sucC, sucD, AACS, and AF1211.

19. The non-naturally occurring microbial organism of claim 1, wherein said acetyl - CoA:acetoacetate-CoA transferase is encoded by one or more genes selected from the group consisting oiatoA, atoD, actA, cgO592, ctfA, ctβ, catl, cat2, cat3, get A, gctB, HPAGl _0676, HPAGl _0677, ScoA, and ScoB.

20. The non-naturally occurring microbial organism of claim 19, wherein said acetyl- CoA:acetoacetate-CoA transferase is encoded by one or more genes selected from the group consisting of atoA, atoD, actA, cgO592, ctfA, ctβ, HPAGl _0676, HPAGl _0677, ScoA, and ScoB.

21. The non-naturally occurring microbial organism of claim 1, wherein said acetoacetyl- CoA hydrolase is encoded by one or more genes selected from the group consisting oϊacotl2, hibeh, BCJ2292, tesB, acot8, tesA, ybgC, paaI, and ybdB.

22. The non-naturally occurring microbial organism of claim 21, wherein said acetoacetyl- CoA hydrolase is encoded by one or more genes selected from the group consisting oiacotl2, hibeh, and tesA.

23. The non-naturally occurring microbial organism of claim 1, wherein said acetoacetate decarboxylase is encoded by one or more genes selected from the group consisting oipdc, pdcl, mdlC, dpgB, ilvB-1, kgd, kdcA, lysA,panD, dmpH, dmpE, xylll, xyllll, Reut_B5691, Reut_B5692, CAD,padl,pofiC, (pad),padC, andpad.

24. The non-naturally occurring microbial organism of claim 23, wherein said acetoacetate decarboxylase is encoded by one or more genes selected from the group consisting of Adc, cbei_3835, CLL_A2135, RBAM_030030.

25. The non-naturally occurring microbial organism of claim 1, wherein said acetone reductase is encoded by one or more genes selected from the group consisting of ArA, akthr2, homo, homl, hom2 JadB JadJ , Hbd2, Hbdl, hbd, HSD17B10,phbB,phaB, Msed_1423, Msed_0399, Msed_0389, Msed_1993, adh, adhA, adh-A, mdh, UhA, Uh, and Mh.

26. The no n- naturally occurring microbial organism of claim 25, wherein said acetone reductase is encoded by one or more genes selected from the group consisting oϊadh, adhA, and adh-A.

27. A method for producing isopropanol comprising culturing a non-naturally occurring microbial organism according to claim 1, under conditions and for a sufficient period of time to produce isopropanol.

28. The method of claim 27, wherein said microbial organism comprises two exogenous nucleic acids, each encoding an isopropanol pathway enzyme.

29. The method of claim 27, wherein said microbial organism comprises three exogenous nucleic acids, each encoding an isopropanol pathway enzyme.

30. The method of claim 27, wherein said microbial organism comprises four exogenous nucleic acids, each encoding an isopropanol pathway enzyme.

31. The method of claim 27, wherein said microbial organism comprises five exogenous nucleic acids, each encoding an isopropanol pathway enzyme.

32. The method of claim 27, wherein said microbial organism comprises six exogenous nucleic acids, each encoding an isopropanol pathway enzyme.

33. The method of claim 27, wherein said microbial organism comprises seven exogenous nucleic acids, each encoding an isopropanol pathway enzyme.

34. The method of claim 27, wherein said microbial organism comprises eight exogenous nucleic acids, each encoding an isopropanol pathway enzyme.

35. The method of claim 27, wherein said non-naturally occurring microbial organism is in a substantially anaerobic culture medium.

36. The method of claim 27, wherein said at least one exogenous nucleic acid is a heterologous nucleic acid.

37. The method of claim 27, wherein said 4-hydroxybutyryl-CoA dehydratase is encoded by one or more genes selected from the group consisting oifumA,fumB,fumC,fumH,fuml, MmcB, MmcC, hmd, BACCAP '_02294, ANACOL_02527, NtherDRAFT_2368, dmdA, dmdB, crt, crtl, paaA,paaB,phaA, phaB, maoC,paaF,paaG, abfl), and Msed_l 220.

38. The method of claim 37, wherein said 4-hydroxybutyryl-CoA dehydratase is encoded by abfD.

39. The method of claim 27, wherein said crotonase is encoded by one or more genes selected from the group consisting oifumA,fumB,fumC,fumH,fuml, MmcB, MmcC, hmd, BACCAP _02294, ANACOL_02527, NtherDRAFT_2368, dmdA, dmdB, crt, crtl,pacιA,pacιB, phaA, phaB, maoC, paaF, paaG, abfl), and Msed_1220.

40. The method of claim 39, wherein said crotonase is encoded by one or more genes selected from the group consisting of crt, crtl , paaA, paaB , phaA, phaB , maoC, paaF, and paaG.

41. The method of claim 27, wherein said 3-hydroxybutyryl-CoA dehydrogenase is encoded by one or more genes selected from the group consisting oithrA, akthr2, homo, homl, hom2, fadBJadJ, Hbd2, Hbdl, hbd, HSD 17B10,phbB, phaB, Msed_1423, Msed_0399, Msed_0389, Msed_1993, adh, adhA, adh-A, mdh, MhA, Uh, and bdh.

42. The method of claim 41, wherein said 3-hydroxybutyryl-CoA dehydrogenase is encoded by one or more genes selected from the group consisting oihbd, Hbd2, Hbdl, Msed_1423, Msed_0399, Msed_0389, Msed_1993, fadB, and fadJ.

43. The method of claim 27, wherein said acetoacetyl-CoA synthetase is encoded by one or more genes selected from the group consisting of sucC, sucD, AACS, AF1211, scs, and PAE3250.

44. The method of claim 43, wherein said acetoacetyl-CoA synthetase is encoded by one or more genes selected from the group consisting of sucC, sucD, AACS, and AF1211.

45. The method of claim 27, wherein said acetyl-CoA:acetoacetate-CoA transferase is encoded by one or more genes selected from the group consisting of atoA, atoD, actA, cgO592, ctfA, ctβ, catl, cat2, cat3, gctA, gctB, HPAGl _0676, HPAGl _0677, ScoA, and ScoB.

46. The method of claim 45, wherein said acetyl-CoA:acetoacetate-CoA transferase is encoded by one or more genes selected from the group consisting of atoA, atoD, actA, cgO592, ctfA, ctβ, HPAGl _0676, HPAGl _0677, ScoA, and ScoB.

47. The method of claim 27, wherein said acetoacetyl-CoA hydrolase is encoded by one or more genes selected from the group consisting oiacotl2, hibch, BC_2292, tesB, acot8, tesA, ybgC, paal, and ybdB.

48. The method of claim 47, wherein said acetoacetyl-CoA hydrolase is encoded by one or more genes selected from the group consisting oiacot!2, hibch, and tesA.

49. The method of claim 27, wherein said acetoacetate decarboxylase is encoded by one or more genes selected from the group consisting oipdc,pdcl, mdlC, dpgB, UvB-I, kgd, kdcA, lysA,panD, dmpH, dmpE, xylll, xyllll, Rent _B5691, Reut_B5692, CAD,padl,pofiC, (pad), padC, and pad.

50. The method of claim 27, wherein said acetoacetate decarboxylase is encoded by one or more genes selected from the group consisting of Adc, cbei_3835, CLL_A2135, RBAM_030030.

51. The method of claim 27, wherein said acetone reductase is encoded by one or more genes selected from the group consisting oithrA, akthr2, homo, homl, hom2,fadB,fadJ, Hbd2, Hbdl, hbd, HSD17B10, phbB,phaB, Msed_1423, Msed_0399, Msed_0389, Msed_1993, adh, adhA, adh-A, mdh, idhA, Idh, and bdh.

52. The method of claim 51 , wherein said acetone reductase is encoded by one or more genes selected from the group consisting oiadh, adhA, and adh-A.

53. A non-naturally occurring microbial organism, comprising a microbial organism having a M-butanol pathway comprising at least one exogenous nucleic acid encoding a w-butanol pathway enzyme expressed in a sufficient amount to produce w-butanol, said w-butanol pathway comprising an enzyme selected from the group consisting of a 4-hydroxybutyryl-CoA dehydratase, a crotonoyl-CoA reductase, a butyryl-CoA reductase (aldehyde forming), a butyraldehyde reductase, and a butyryl-CoA reductase (alcohol forming).

54. The non-naturally occurring microbial organism of claim 53, wherein said microbial organism comprises two exogenous nucleic acids, each encoding an w-butanol pathway enzyme.

55. The non-naturally occurring microbial organism of claim 53, wherein said microbial organism comprises three exogenous nucleic acids, each encoding an w-butanol pathway enzyme.

56. The non-naturally occurring microbial organism of claim 53, wherein said microbial organism comprises four exogenous nucleic acids, each encoding an w-butanol pathway enzyme.

57. The non-naturally occurring microbial organism of claim 53, wherein said at least one exogenous nucleic acid is a heterologous nucleic acid.

58. The non-naturally occurring microbial organism of claim 53, wherein said non-naturally occurring microbial organism is in a substantially anaerobic culture medium.

59. The non-naturally occurring microbial organism of claim 53, wherein said A- hydroxybutyryl-CoA dehydratase is encoded by one or more genes selected from the group consisting oifumA,fumB,fumC,fumH,fuml, MmcB, MmcC, hmd, BACCAP '_02294, ANACOL_02527, NtherDRAFT_2368, dmclA, dmclB, crt, crtl, paaA,paaB,phaA,phaB, maoC, paaF,paaG, abfD, and Msed_l 220.

60. The non-naturally occurring microbial organism of claim 59, wherein said A- hydroxybutyryl-CoA dehydratase is encoded by abfD.

61. The non-naturally occurring microbial organism of claim 53, wherein said crotonoyl- CoA reductase is encoded by one or more genes selected from the group consisting oibcd, etfA, etβ, Ter, TDEO 597 , IBD, RPA3448, FadH, and enr.

62. The non-naturally occurring microbial organism of claim 61, wherein said crotonoyl-

CoA reductase is encoded by one or more genes selected from the group consisting oibcd, etfA, etβ, Ter, and TDEO 597.

63. The non-naturally occurring microbial organism of claim 53, wherein said butyryl-CoA reductase (aldehyde forming) is encoded by one or more genes selected from the group consisting oϊacrl, sucD, bphG, adhE, Msed_0709, mcr, asd-2, Saci_2370, Aid, and eutE.

64. The non-naturally occurring microbial organism of claim 63, wherein said butyryl-CoA reductase (aldehyde forming) is encoded by one or more genes selected from the group consisting of sucD, bphG, Msed_0709, mcr, and Aid.

65. The non-naturally occurring microbial organism of claim 53, wherein said butyraldehyde reductase is encoded by one or more genes selected from the group consisting oϊalrA, ADH2, yqhD, bdh I, bdh II, adhA, 4hbd, adhl, P84067, mmsb, dhat, and 3hidh.

66. The non-naturally occurring microbial organism of claim 65, wherein said butyraldehyde reductase is encoded by one or more genes selected from the group consisting oialrA, ADH2, yqhD, bdh I, bdh II, 4hbd, adhl, and mmsb.

67. The non-naturally occurring microbial organism of claim 53, wherein said butyryl-CoA reductase (alcohol forming) is encoded by one or more genes selected from the group consisting oiadhE, adhE2, mcr, Rcas_2929, NAPl _02720, MGP2080_00535, and FAR.

68. The non-naturally occurring microbial organism of claim 67, wherein said butyryl-CoA reductase (alcohol forming) is encoded by one or more genes selected from the group consisting oiadhE2, mcr, and FAR.

69. A method for producing w-butanol comprising culturing a non-naturally occurring microbial organism according to claim 53, under conditions and for a sufficient period of time to produce w-butanol.

70. The method of claim 69, wherein said microbial organism comprises two exogenous nucleic acids, each encoding an w-butanol pathway enzyme.

71. The method of claim 69, wherein said microbial organism comprises three exogenous nucleic acids, each encoding an w-butanol pathway enzyme.

72. The method of claim 69, wherein said microbial organism comprises four exogenous nucleic acids, each encoding an w-butanol pathway enzyme.

73. The method of claim 69, wherein said at least one exogenous nucleic acid is a heterologous nucleic acid.

74. The method of claim 69, wherein said non-naturally occurring microbial organism is in a substantially anaerobic culture medium.

75. The method of claim 69, wherein said 4-hydroxybutyryl-CoA dehydratase is encoded by one or more genes selected from the group consisting oifumA,fumB,fumC,fumH,fuml, MmcB, MmcC, hmd, BACCAP '_02294, ANACOL_02527, NtherDRAFT_2368, dmdA, dmdB, crt, crtl, paaA,paaB,phaA, phaB, maoC, paaF, paaG, abfD, and Msed_l 220.

76. The method of claim 75, wherein said 4-hydroxybutyryl-CoA dehydratase is encoded by abfD.

77. The method of claim 69, wherein said crotonoyl-CoA reductase is encoded by one or more genes selected from the group consisting oϊbcd, etfA, etjB, Ter, TDE0597, IBD, RPA3448, FadH, and enr.

78. The method of claim 77, wherein said crotonoyl-CoA reductase is encoded by one or more genes selected from the group consisting oϊbcd, etfA, etfB, Ter, and TDE0597.

79. The method of claim 69, wherein said butyryl-CoA reductase (aldehyde forming) is encoded by one or more genes selected from the group consisting oiacrl, sucD, bphG, adhE, Msed_0709, mcr, asd-2, Saci_2370, Aid, and eutE.

80. The method of claim 79, wherein said butyryl-CoA reductase (aldehyde forming) is encoded by one or more genes selected from the group consisting of sucD, bphG, Msed_0709, mcr, and Aid.

81. The method of claim 69, wherein said butyraldehyde reductase is encoded by one or more genes selected from the group consisting oialrA, ADH2, yqhD, bdh I, bdh II, adhA, 4hbd, adhl, P84067, mmsb, dhat, and 3hidh.

82. The method of claim 81 , wherein said butyraldehyde reductase is encoded by one or more genes selected from the group consisting oialrA, ADH2, yqhD, bdh I, bdh II, 4hbd, adhl, and mmsb.

83. The method of claim 69, wherein said butyryl-CoA reductase (alcohol forming) is encoded by one or more genes selected from the group consisting oladhE, adhE2, mcr, Rcas_2929, NAPl _02720, MGP2080JJ0535, and FAR.

84. The method of claim 83, wherein said butyryl-CoA reductase (alcohol forming) is encoded by one or more genes selected from the group consisting oiadhE2, mcr, and FAR.

85. A non-naturally occurring microbial organism, comprising a microbial organism having an isobutanol pathway comprising at least one exogenous nucleic acid encoding an isobutanol pathway enzyme expressed in a sufficient amount to produce isobutanol, said isobutanol pathway comprising an enzyme selected from the group consisting of a 4-hydroxybutyryl-CoA dehydratase, a crotonoyl-CoA reductase, an isobutyryl-CoA mutase, a 4-hydroxybutyryl-CoA mutase, a 3-hydroxyisobutyryl-CoA dehydratase, a methacrylyl-CoA-reductase, an isobutyryl- CoA reductase (aldehyde forming), an isobutyraldehyde reductase, and an isobutyryl-CoA reductase (alcohol forming).

86. The no n- naturally occurring microbial organism of claim 85, wherein said microbial organism comprises two exogenous nucleic acids, each encoding an isobutanol pathway enzyme.

87. The no n- naturally occurring microbial organism of claim 85, wherein said microbial organism comprises three exogenous nucleic acids, each encoding an isobutanol pathway enzyme.

88. The non-naturally occurring microbial organism of claim 85, wherein said microbial organism comprises four exogenous nucleic acids, each encoding an isobutanol pathway enzyme.

89. The non-naturally occurring microbial organism of claim 85, wherein said microbial organism comprises five exogenous nucleic acids, each encoding an isobutanol pathway enzyme.

90. The non-naturally occurring microbial organism of claim 85, wherein said at least one exogenous nucleic acid is a heterologous nucleic acid.

91. The non-naturally occurring microbial organism of claim 85, wherein said non-naturally occurring microbial organism is in a substantially anaerobic culture medium.

92. The non-naturally occurring microbial organism of claim 85, wherein a set of isobutanol pathway enzymes comprises a 4-hydroxybutyryl-CoA dehydratase, a crotonoyl-CoA reductase, an isobutyryl-CoA mutase, an isobutyryl-CoA reductase (aldehyde forming), and an isobutyraldehyde reductase.

93. The non-naturally occurring microbial organism of claim 85, wherein a set of isobutanol pathway enzymes comprises a 4-hydroxybutyryl-CoA dehydratase, a crotonoyl-CoA reductase, an isobutyryl-CoA mutase, and an isobutyryl-CoA reductase (alcohol forming).

94. The no n- naturally occurring microbial organism of claim 85, wherein a set of isobutanol pathway enzymes comprises a 4-hydroxybutyryl-CoA mutase, a 3 -hydro xyisobutyryl-CoA dehydratase, a methacrylyl-CoA-reductase, an isobutyryl-CoA reductase (aldehyde forming), and an isobutyraldehyde reductase.

95. The non-naturally occurring microbial organism of claim 85, wherein a set of isobutanol pathway enzymes comprises a 4-hydroxybutyryl-CoA mutase, a 3 -hydro xyisobutyryl-CoA dehydratase, a methacrylyl-CoA-reductase, and an isobutyryl-CoA reductase (alcohol forming).

96. The non-naturally occurring microbial organism of claim 85, wherein said A- hydroxybutyryl-CoA dehydratase is encoded by one or more genes selected from the group consisting oϊfumA,fumB,fumC,fumH,fuml, MmcB, MmcC, hmd, BACCAP '_02294,

ANACOL_02527, NtherDRAFT_2368, dmclA, dmclB, crt, crtl, paaA,paaB,phaA,phaB, maoC, paaF,paaG, abfl), and Msed_l 220.

97. The non-naturally occurring microbial organism of claim 96, wherein said A- hydroxybutyryl-CoA dehydratase is encoded by abfl).

98. The non-naturally occurring microbial organism of claim 85, wherein said crotonoyl-

CoA reductase is encoded by one or more genes selected from the group consisting oϊbcd, etfA, etβ, Ter, TDEO 597, IBD, RP A3448, FadH, and enr.

99. The non-naturally occurring microbial organism of claim 98, wherein said crotonoyl- CoA reductase is encoded by one or more genes selected from the group consisting oibcd, etfA, etβ, Ter, and TDE0597.

100. The non-naturally occurring microbial organism of claim 85, wherein said isobutyryl- CoA mutase is encoded by one or more genes selected from the group consisting oiicm, icmB, icmA, Mpe_B0538, Mpe_B0541, scpA, mutA, mutB, mcmA, mcmB, sbm, SARI_04585, YfreA_01000861, argK, PPA0597, and meciB.

101. The non-naturally occurring microbial organism of claim 100, wherein said isobutyryl- CoA mutase is encoded by one or more genes selected from the group consisting oiicmB, icmA, Mpe_B0538, and Mpe_B0541.

102. The non-naturally occurring microbial organism of claim 85, wherein said A- hydroxybutyryl-CoA mutase is encoded by one or more genes selected from the group consisting oiicm, icmB, icmA, Mpe_B0538, Mpe_B0541, scpA, mutA, mutB, mcmA, mcmB, sbm, SARI_04585, YfreAJ) 1000861, argK, PPA0597, and meciB.

103. The no n- naturally occurring microbial organism of claim 102, wherein said A- hydroxybutyryl-CoA mutase is encoded by one or more genes selected from the group consisting oiicmB, icmA, Mpe_B0538, and Mpe_B0541.

104. The no n- naturally occurring microbial organism of claim 85, wherein said 3- hydroxyisobutyryl-CoA dehydratase is encoded by one or more genes selected from the group consisting oifumA,fumB,fumC,fumH,fuml, MmcB, MmcC, hmd, BACCAP _ 02294, ANACOL_02527, NtherDRAFT_2368, dmclA, dmclB, crt, crtl, paaA,paaB,phaA,phaB, maoC, paaF, paaG, abfD, and Msed_1220.

105. The non-naturally occurring microbial organism of claim 104, wherein said 3- hydroxyisobutyryl-CoA dehydratase is encoded by one or more genes selected from the group consisting of crt, crtl , paaA, paaB , phaA, phaB, maoC,paaF, and paaG.

106. The non-naturally occurring microbial organism of claim 85, wherein said methacrylyl- CoA reductase is encoded by one or more genes selected from the group consisting oibcd, etfA, etβ, Ter, TDEO 597 , IBD, RPA3448, FadH, and enr.

107. The non-naturally occurring microbial organism of claim 106, wherein said methacrylyl- CoA reductase is encoded by one or more genes selected from the group consisting oibcd, etfA, etβ, Ter, and TDEO 597.

108. The non-naturally occurring microbial organism of claim 85, wherein said isobutyryl- CoA reductase (aldehyde forming) is encoded by one or more genes selected from the group consisting oiacrl, sucD, bphG, adhE, Msed_0709, mcr, asd-2, Saci_2370, Aid, and eutE.

109. The non-naturally occurring microbial organism of claim 108, wherein said isobutyryl- CoA reductase (aldehyde forming) is encoded by one or more genes selected from the group consisting of sucD, bphG, Msed_0709, mcr, and Aid.

110. The non-naturally occurring microbial organism of claim 85, wherein said isobutyraldehyde reductase is encoded by one or more genes selected from the group consisting of alrA, ADH2, yqhD, bdh I, bdh II, cidhA, 4hbd, adhl, P84067, mmsb, dhat, and 3hidh..

111. The no n- naturally occurring microbial organism of claim 110, wherein said isobutyraldehyde reductase is encoded by one or more genes selected from the group consisting of air A, ADH2, yqhD, bdh I, bdh II, 4hbd, adhl, P84067, and mmsb.

112. The non-naturally occurring microbial organism of claim 85, wherein said isobutyryl- CoA reductase (alcohol forming) is encoded by one or more genes selected from the group consisting oiadhE, adhE2, mcr, Rcas_2929, NAPl_02720, MGP2080_00535, and FAR.

113. The non-naturally occurring microbial organism of claim 112, wherein said isobutyryl- CoA reductase (alcohol forming) is encoded by one or more genes selected from the group consisting oiadhE2, mcr, and FAR.

114. A method for producing isobutanol comprising culturing a non-naturally occurring microbial organism according to claim 85, under conditions and for a sufficient period of time to produce isobutanol.

115. The method of claim 114, wherein said microbial organism comprises two exogenous nucleic acids, each encoding an isobutanol pathway enzyme.

116. The method of claim 114, wherein said microbial organism comprises three exogenous nucleic acids, each encoding an isobutanol pathway enzyme.

117. The method of claim 114, wherein said microbial organism comprises four exogenous nucleic acids, each encoding an isobutanol pathway enzyme.

118. The method of claim 114, wherein said at least one exogenous nucleic acid is a heterologous nucleic acid.

119. The method of claim 114, wherein said non-naturally occurring microbial organism is in a substantially anaerobic culture medium.

120. The method of claim 114, wherein a set of isobutanol pathway enzymes comprises a 4- hydroxybutyryl-CoA dehydratase, a crotonoyl-CoA reductase, an isobutyryl-CoA mutase, an isobutyryl-CoA reductase (aldehyde forming), and an isobutyraldehyde reductase.

121. The method of claim 114, wherein a set of isobutanol pathway enzymes comprises a 4- hydroxybutyryl-CoA dehydratase, a crotonoyl-CoA reductase, an isobutyryl-CoA mutase, and an isobutyryl-CoA reductase (alcohol forming).

122. The method of claim 114, wherein a set of isobutanol pathway enzymes comprises a A- hydroxybutyryl-CoA mutase, a 3-hydroxyisobutyryl-CoA dehydratase, a methacrylyl-CoA- reductase, an isobutyryl-CoA reductase (aldehyde forming), and an isobutyraldehyde reductase.

123. The method of claim 114, wherein a set of isobutanol pathway enzymes comprises a A- hydroxybutyryl-CoA mutase, a 3-hydroxyisobutyryl-CoA dehydratase, a methacrylyl-CoA- reductase, and an isobutyryl-CoA reductase (alcohol forming).

124. The method of claim 114, wherein said 4-hydroxybutyryl-CoA dehydratase is encoded by one or more genes selected from the group consisting oifumA,fumB,fumC,fumH,fuml, MmcB, MmcC, hmd, BACCAP '_02294, ANACOLJJ2527, NtherDRAFT_2368, dmdA, dmdB, crt, crtl, paaA, paaB, phaA, phaB, maoC, paaF, paaG, abfl), and Msed_1220.

125. The method of claim 124, wherein said 4-hydroxybutyryl-CoA dehydratase is encoded by abfD.

126. The method of claim 114, wherein said crotonoyl-CoA reductase is encoded by one or more genes selected from the group consisting oibcd, etfA, etJB, Ter, TDE0597, IBD, RPA3448, FadH, and enr.

127 ^'. The method of claim 126, wherein said crotonoyl-CoA reductase is encoded by one or more genes selected from the group consisting oibcd, etfA, etjB, Ter, and TDE0597.

128. The method of claim 114, wherein said isobutyryl-CoA mutase is encoded by one or more genes selected from the group consisting oiicm, icmB, icmA, Mpe_B0538, Mpe_B0541, scpA, mutA, mutB, mcmA, mcmB, sbm, SARI_04585, YfreAJJl 000861, argK, PPA0597, and meaB.

129. The method of claim 128, wherein said isobutyryl-CoA mutase is encoded by one or more genes selected from the group consisting oiicmB, icmA, Mpe_B0538, and Mpe_B0541.

130. The method of claim 114, wherein said 4-hydroxybutyryl-CoA mutase is encoded by one or more genes selected from the group consisting oiicm, icmB, icmA, Mpe_B0538, Mpe_B0541, scpA, mutA, mutB, mcmA, mcmB, sbm, SARI_04585, YfreAJJl 000861, argK, PPA0597, and meaB.

131. The method of claim 130, wherein said 4-hydroxybutyryl-CoA mutase is encoded by one or more genes selected from the group consisting oiicmB, icmA, Mpe_B0538, and Mpe_B0541.

132. The method of claim 114, wherein said 3 -hydro xyisobutyryl-CoA dehydratase is encoded by one or more genes selected from the group consisting oifumA,fumB,fumC,fumH, fuml, MmcB, MmcC, hmd, BACCAP '_02294, ANACOL_02527, NtherDRAFT_2368, dmdA, dmdB, crt, crtl , paaA, paaB, phaA, phaB, maoC, paaF, paaG, abfD, and Msed_l 220.

133. The method of claim 132, wherein said 3 -hydro xyisobutyryl-CoA dehydratase is encoded by one or more genes selected from the group consisting of crt, crtl,paaA,paaB, phaA,phaB, maoC,paaF, and paaG.

134. The method of claim 114, wherein said methacrylyl-CoA reductase is encoded by one or more genes selected from the group consisting oibcd, etfA, etJB, Ter, TDE0597, IBD, RPA3448, FadH, and enr.

135. The method organism of claim 134, wherein said methacrylyl-CoA reductase is encoded by one or more genes selected from the group consisting oibcd, etfA, etfB, Ter, and TDE0597.

136. The method of claim 114, wherein said isobutyryl-CoA reductase (aldehyde forming) is encoded by one or more genes selected from the group consisting oiacrl, sucD, bphG, adhE, Msed_0709, mcr, asd-2, Saci_2370, Aid, and eutE.

137. The method of claim 136, wherein said isobutyryl-CoA reductase (aldehyde forming) is encoded by one or more genes selected from the group consisting of sucD, bphG, Msed_0709, mcr, and Aid.

138. The method of claim 114, wherein said isobutyraldehyde reductase is encoded by one or more genes selected from the group consisting oialrA, ADH2, yqhD, bdh I, bdh II, adhA, 4hbd, adhl, P84067, mmsb, dhat, and 3hidh..

139. The method of claim 138, wherein said isobutyraldehyde reductase is encoded by one or more genes selected from the group consisting oialrA, ADH2, yqhD, bdh I, bdh II, 4hbd, adhl, P84067, and mmsb.

140. The method organism of claim 114, wherein said isobutyryl-CoA reductase (alcohol forming) is encoded by one or more genes selected from the group consisting oiadhE, adhE2, mcr, Rcas_2929, NAPl_02720, MGP2080_00535, and FAR.

141. The method of claim 140, wherein said isobutyryl-CoA reductase (alcohol forming) is encoded by one or more genes selected from the group consisting oiadhE2, mcr, and FAR.