WO2010117281A1 - Composition alimentaire et méthode d'alimentation de gammaridés - Google Patents

Composition alimentaire et méthode d'alimentation de gammaridés Download PDF

Info

Publication number
WO2010117281A1
WO2010117281A1 PCT/NO2010/000128 NO2010000128W WO2010117281A1 WO 2010117281 A1 WO2010117281 A1 WO 2010117281A1 NO 2010000128 W NO2010000128 W NO 2010000128W WO 2010117281 A1 WO2010117281 A1 WO 2010117281A1
Authority
WO
WIPO (PCT)
Prior art keywords
fatty acids
gammaridae
feed
dry weight
omega
Prior art date
Application number
PCT/NO2010/000128
Other languages
English (en)
Inventor
Tor Erling Moe
Original Assignee
Fora-Tek As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fora-Tek As filed Critical Fora-Tek As
Priority to EP10719657A priority Critical patent/EP2416667A1/fr
Publication of WO2010117281A1 publication Critical patent/WO2010117281A1/fr

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
    • A61K31/19—Carboxylic acids, e.g. valproic acid
    • A61K31/20—Carboxylic acids, e.g. valproic acid having a carboxyl group bound to a chain of seven or more carbon atoms, e.g. stearic, palmitic, arachidic acids
    • A61K31/202—Carboxylic acids, e.g. valproic acid having a carboxyl group bound to a chain of seven or more carbon atoms, e.g. stearic, palmitic, arachidic acids having three or more double bonds, e.g. linolenic
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/158—Fatty acids; Fats; Products containing oils or fats
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/115—Fatty acids or derivatives thereof; Fats or oils
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish
    • Y02A40/818—Alternative feeds for fish, e.g. in aquacultures

Definitions

  • the present invention relates to a method for production of fatty acids with more than three double bonds as defined in the preamble of claim 1 and to a method for the cultivation of Gammaridae.
  • the present invention also relates to a fish feed comprising Gammaridae, a lipid product extracted or produced from a Gammaridae, a use of the lipid product, as well as to a feed for the production of Gammaridae and the use of the feed.
  • marine lipids comprising highly unsaturated fatty acids such as omega-3 fatty acids on the world market.
  • Fish meals and fish oils are the traditional major sources for these lipids. They are mainly derived from wild caught marine fish such as capelin, herring, anchovy, sardine fisheries etc.
  • a major application of marine lipids is their use as feed ingredients in fish farming.
  • Especially marine fish have a high requirement for the essential omega-3 fatty acids for normal growth and development, which is largely covered by fish oil addition to the feed or by enrichment of prey organisms used in the cultivation.
  • omega-3 fatty acids such as docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) and for omega-6 fatty acids such as arachidonic acid (ARA).
  • DHA docosahexaenoic acid
  • EPA eicosapentaenoic acid
  • ARA arachidonic acid
  • Marine lipids comprising the highly unsaturated fatty acids (HUFA) such as omega-3 fatty acids (e.g. DHA, EPA) can also be found at lower fauna levels of the marine food web such as in copepods and krill.
  • omega-3 fatty acids e.g. DHA, EPA
  • these organisms are in general known to be dependent on an external supply of these essential fatty acids in their diet which often consists of other smaller marine organisms comprising these fatty acids or marine microalgae with a high level of these omega-3 fatty acids and omega-6 fatty acids.
  • Species of Gammaridae which are also Crustaceans present in the lower food web and are belonging to the family of amphipods, were shown to comprise omega-3 and omega-6 fatty acids.
  • Gammaridae are not effectively exploitable by wild catches and only very recently efforts have been done to cultivate some marine species under controlled conditions.
  • the main objective of the present invention is to develop a new method for producing omega-3 fatty acids such as DHA and EPA and long-chain omega-6 fatty acids such as ARA without the need for marine lipids.
  • Another objective of the invention is to make use of vegetable resources as a raw material basis for the production of marine lipids.
  • Yet another objective of the invention is to develop a new alternative source for marine lipids such as for human consumption or for aquaculture purposes which is independent of the addition of marine lipids during the production process.
  • a method for production of fatty acids with more than three double bonds in Gammaridae whereby said Gammaridae are fed a feed comprising saturated and/or unsaturated fatty acids which have no more than 2 double bonds and wherein a portion of said supplied fatty acids is converted to fatty acids with more than three double bonds.
  • a method for the cultivation of Gammaridae whereby said Gammaridae are fed a feed which comprises unsaturated fatty acids having no more than 2 double bonds and whereby the Gammaridae endogenously produce polyunsaturated fatty acids having more than three double bonds.
  • said fatty acids are produced through unsaturation and/or elongation of the supplied fatty acids in the feed.
  • the produced fatty acids have 20 carbon atoms or more.
  • the feed further comprises unsaturated fatty acids with 3 double bonds.
  • the feed does not comprise any highly unsaturated fatty acids chosen from the group of DHA, EPA and/or ARA.
  • the fatty acids in the feed are derived from a non-marine lipid material, preferably derived from or provided as seeds, fruits and/or other plant material from a terrestrial plant chosen from the group of soya bean, sun flower, raps, palm, coconut, corn, peanut and/or flax, most preferable from soya.
  • the used feed is free of any marine lipid material.
  • the produced unsaturated fatty acids have 20 carbon atoms or more and wherein the produced fatty acids have 4, 5 or 6 double bonds.
  • the produced fatty acids are belonging to the group of omega-3 and/or omega-6 fatty acids, preferably fatty acids chosen from DHA, EPA and ARA.
  • the comprised polyunsaturated fatty acids in the feed are substantially only C18 omega-6 fatty acids and if present the other omega-6 and/or omega-3 fatty acids account for less than 0.5 %, preferably less than 0.05%, most preferably 0.02% or less of the total fatty acids.
  • the content of C18 omega-6 fatty acids in the feed is no more than 3% of the total fatty acids.
  • the said cultivated Gammaridae is the species Gammarus oceanicus which has a content of omega-3 fatty acids of at least 22 mg/g dry weight and an omega-6 content of at least 11 mg/g dry weight, preferably comprising at least 7 mg DHA/g dry weight, 10 mg EPA/ g dry weight and at least 2.5 mg ARA per g dry weight.
  • said cultivated Gammaridae is the species Gammarus locusta, which has a content of omega-3 fatty acids of at least 12 mg/g dry weight, preferably more than 24 mg/g dry weight and an omega-6 content of at least 3 mg/g dry weight, preferably more than 6 mg/g dry weight, wherein preferably at least 2 mg /g dry weight, more preferred at least 7 mg/ g dry weight is DHA, at least 6 mg/ g dry weight, more preferred at least 11 mg/g dry weight is EPA and at least 0.5 mg per g dry weight is ARA, more preferred at least 0.7 mg/g dry weight.
  • the said cultivated Gammaridae is G. locusta, which has at least 3% of the total fatty acids as DHA, preferably at least 9% DHA of the total fatty acids, at least 7 % of the total fatty acids as EPA, preferably at least 15 % EPA of the total fatty acids and/or at least 0.9% of the total fatty acids as ARA, preferably at least 1.9 % ARA of the total fatty acids.
  • the produced Gammaridae have a lipid content of at least 125 mg lipid/ g dry weight, preferably of at least 135 mg lipid/g dry weight, more preferably of at least 140 mg/ dry weight.
  • a lipid product extracted or produced from a Gammaridae whereby said Gammaridae are fed a feed which comprises unsaturated fatty acids having no more than 2 double bonds and whereby the Gammaridae endogenously produce polyunsaturated fatty acids having more than three double bonds.
  • a lipid product which is extracted or produced from a Gammaridae wherein said Gammaridae are produced according to any of the methods described in the previous paragraphs.
  • the fatty acid composition of the lipid product is corresponding to the composition of the produced Gammaridae according to any of the preceding paragraphs or to claim 42, preferably the fatty acids composition of DHA, EPA and/or ARA is according to any of the Tables 4-7.
  • the invention also relates to a lipid product extracted or produced from a Gammaridae wherein said Gammaridae are fed a feed according to the below following during cultivation.
  • a fish feed comprising Gammaridae wherein said Gammaridae are produced according to any of the preceding paragraphs.
  • the invention relates to the use of a lipid product produced from Gammaridae obtainable by a method according to any of the preceding claims as an ingredient in feed for animal production, preferably aquaculture farming, most preferably for farming of salmon or marine fish.
  • the invention relates to the use of a lipid product produced from Gammaridae obtainable by a method according to any of preceding claims for human consumption, preferably as nutrient supplement, as a health product, as a food ingredient or for the preparation of a pharmaceutical composition.
  • the invention further relates to a use of a lipid product produced from Gammaridae obtainable by a method according to any of preceding paragraphs for manufacturing of a pharmaceutical composition for the treatment and/or prevention of a cardiovascular disease, cerebral infarction, hyperlipidemia, arthrosclerosis, hypertension, tumor illness, diabetes, rheumatic arthritis, multiple sclerosis, psoriasis, arthritis, asthma, and/or cancer.
  • the invention relates to a feed for the production of Gammaridae wherein said feed comprises non-marine lipid material.
  • said lipid material comprises unsaturated fatty acids having no more than 2 double bonds and wherein said lipid material is preferably from a terrestrial plant.
  • the lipid material further comprises unsaturated fatty acids with 3 double bonds.
  • the feed does not comprise any highly unsaturated fatty acids chosen from the group of DHA, EPA and/or ARA or wherein the sum of omega-3 fatty acids is less than 0.1mg/g dry weight, preferably 0.05 mg/g or less.
  • said lipids are comprised in or derived from seeds, fruits and/or other plant material from a terrestrial plant chosen from the group of soya bean, sun flower, raps, palm, coconut, corn, peanut and/or flax, most preferable from soya.
  • the comprised lipids are solely derived from a non-marine lipid material, preferably from a terrestrial plant.
  • the feed further comprises other ingredients from a plant.
  • the feed is for a marine Gammaridae, preferably chosen from the species Gammarus oceanicus and Gammarus locusta.
  • said feed further comprises other common feed ingredients such as proteins, carbohydrates, vitamins and/or minerals.
  • the comprised polyunsaturated fatty acids are substantially only omega-6 fatty acids with 18 carbon atoms and if present fatty acids with 20 or more carbon atoms belonging to the group of omega-6 and/or omega-3 fatty acids are no more than 0.5 %, preferably no more than 0.05%, most preferably 0.02% or less of the total fatty acids.
  • the comprised polyunsaturated fatty acids account for less than 3 % of the total fatty acids.
  • the sum of saturated fatty acids, monounsaturated fatty acids, C18:2n-6 and C18:3n-6 account for more than 95%, preferably for at least 99% of the total fatty acids in the comprised lipids.
  • composition of the fatty acids in said lipid is substantially as shown in any of the Tables 1 , 2 or 3.
  • said feed further comprises lipids or other material derived from an animal, preferably from an aquatic organism, more preferable form a marine animal chosen from fish, zooplankton or a crustacean and/or from a marine plant chosen from a macroalgae such as a sea weed species or a microalgae.
  • Another aspect of the present invention relates to the use of a feed according to any preceding paragraphs for the cultivation of Gammaridae, preferably for the intensive cultivation.
  • Yet another aspect of the invention is related to a use of a feed according to any of the preceding paragraphs for increasing the lipid content of the produced Gammaridae, preferably to at least 125 mg lipid/ g dry weight, more preferably to at least 135 mg lipid/g dry weight, most preferably to at least 140 mg/ dry weight and/or for delaying the spawning/reproduction of Gammaridae.
  • Yet another aspect of the invention is related to a use of a feed according to any of the preceding paragraphs for the endogenous production of omega-3 fatty acids, preferably of DHA and/or EPA and/or of the omega-6 fatty acids ARA in Gammaridae when used in their cultivation process.
  • the sum of the fatty acids DHA, EPA and ARA in Gammaridae cultivated with said feed is at least 8.5 mg/g dry weight, preferable at least 19.5 mg/ g dry weight, most preferable at least 20.5 mg/ g dry weight of the Gammaridae, and/or the sum of said fatty acids in the Gammaridae is at least 12 % of the total fatty acids, preferably at least 17.5 % of the total fatty acids, most preferably at least 25.5 % of the total fatty acids.
  • Another aspect of the present invention is related to the use of a feed according to any of the preceding paragraphs for the production of a lipid product comprising highly unsaturated long chain omega-3 fatty acids and/or arachidonic acid by cultivating Gammaridae with said feed.
  • the present invention also relates to the use of a feed in a method according to any of the preceding paragraphs.
  • the present invention surprisingly allows to produce omega-3 fatty acids with more than 3 double bonds such as DHA and EPA and long chain omega-6 fatty acid e.g. ARA through Gammaridae by using a terrestrial plant, fruit or vegetable seed based diet, which is free or substantially free of these highly unsaturated omega-3 fatty acids or omega-6 fatty acids with more than 18 carbon atoms.
  • omega-3 fatty acids with more than 3 double bonds
  • omega-6 fatty acids with more than 18 carbon atoms e.g. ARA
  • Gammaridae can produce highly unsaturated omega-3 fatty acids and omega-6 fatty acids, when practically no polyunsaturated fatty acids were present in the diet.
  • Gammaridae are a family of amphipods inhabiting fresh to marine waters. Many members of the family are largely benthic.
  • the new feed according to the present invention can thus be used for the production of Gammaridae and of a derived lipid fraction which has a high degree of the omega- 3 fatty acids DHA and EPA and omega-6 fatty acid ARA 1 in spite of the fact that the diet used in the Gammaridae production can be substantially free of these fatty acids and furthermore even practically free any omega-6 fatty acid such as linoleic acid (18:2n-6).
  • a new alternative production method and source for marine omgea-3 fatty acids and omega-6 fatty acids is provided.
  • the products of the invention represent a totally new source and method for the production of marine lipids.
  • the diet according to the present invention can also be used to achieve a higher lipid content in Gammaridae than in wild caught animals or animals fed a fish raw material based diet with a comparable lipid content, which is a further advantage for the production of a lipid product.
  • the lipids produced according to the present invention can be used in nutritional compositions, as well as in health products and in pharmaceutical compositions.
  • Highly unsaturated fatty acids such as DHA and EPA are shown to have beneficial effects in the treatment and/or prevention of certain diseases such as cardiovascular diseases, cerebral infarction, hyperlipidemia, arthrosclerosis, hypertension, tumor illness, diabetes, rheumatic arthritis, multiple sclerosis, psoriasis, arthritis, asthma, cancer as e.g.
  • Figure 1 shows the fatty acid profiles of the diets used in the feeding experiments (AL; salmon filet, SB; soya beans, CO; coconut (data for CO from Nagaraju and Belur, 2008)) and in Gammaridae fed the respective diets.
  • A Illustrates the absolute values (mg/g dry weight) and B the relative distribution of fatty acids (% of total amount).
  • MUFA monounsaturated fatty acids, SFA; saturated fatty acids, NFAL; lipids that are not fatty acids.
  • G. locusta The effect of a diet based on vegetable raw material was also tested in another species belonging to the Gammaridae family being the species Gammarus locusta.
  • two different groups of G. locusta were fed a diet either solely consisting of soya beans or of coconut fruit (Cocos nucifera).
  • coconut fruit In the coconut diet, fruits were cut in pieces having a size of about 0.5 - 1.5 mm.
  • G. locusta were collected in the Northern Norway (area west of Troms ⁇ ).
  • the lipid content and fatty acid composition of the soya beans are given in Table 1 and 2 and of coconut in Table 3, respectively.
  • the feeding experiment 2 and sampling was carried out as described for experiment 1.
  • the experiment lasted for 44 days.
  • Lipids and fatty acids were analyzed according to Rainuzzo et al. (1992) by using a modified method of Bligh and Dyer (1959) for extraction of lipids and a method of Metcalfe et al. (1966) for methylation of fatty acids.
  • FAMEs were quantified in a gas chromatograph (Carlo Erba HRGC 5160) connected to a Shimadzu-Chromatopac C R3A integrator. The total lipid content was analyzed gravimetrically (mg/g dry weight).
  • G. oceanicus a diet solely consisting of soya beans resulted in a final lipid content of 140.4 mg per g dry weight which was more than the double of that in animals fed a diet consisting of salmon filet (68.2 mg lipid per g dry weight; Fig. 1 A and Table 4) when fed for a comparable period of time. This was the case in spite of the fact that the soya bean diet had a lower lipid content than the salmon filet being 87.2 mg/g and 238.8 mg/g, respectively.
  • G. oceanicus sampled from the wild have a typical lipid content of 65-82 mg/g (not shown).
  • the new diet consisting of soya beans
  • the higher lipid content in Gammaridae fed the soya bean diet can be related to an observed delay in spawning and thus reproductive activity, which was probably caused by the diet.
  • Spawning is assumed to reduce the lipid content of the animals, since the lipid reserves are potentially used in egg production.
  • Table 4 Content of lipids and fatty acids (mg/g dry weight; means ⁇ standard deviation) in G. oceanicus fed Atlantic salmon filet (AL) and soya beans (SB). FA Fatty acids.
  • DHAVEPA 0,69 ⁇ 0,00 0,68 ⁇ 0,01 non Hufa n-3 1,13 ⁇ 1,10 2,81 ⁇ 0,03
  • Gammarus locusta solely fed a coconut based diet in experiment 2 had as much as 27.7 mg polyunsaturated fatty acids per g dry weight compared to about 19 mg/g dry weight in the treatment fed soya beans (Table 6, Figure 1A).
  • the lipid content obtained was fairly similar for both treatments and in the range of what was achieved for G. oceanicus fed soya beans in experiment 1.
  • Gammaridae belonging to the coconut treatment comprised 9.5 % of the total fatty acids as DHA, 15.2 % as EPA and 0.9 % ARA (Table 7, Figure 1B), while the diet did not include any of these fatty acids (Table 3). This corresponds to a DHA content of 7.4 mg/g and EPA of 11.7 mg/g and 0.72 mg ARA /g dry weight in the animals fed coconut fruits. Feeding of the soya bean diet resulted in 3.3 % DHA, 7.2 % EPA and 1.9 % ARA of the total fatty acids (Table 7), corresponding to 2.37 mg DHA per g dry weight, 6.04 mg EPA /g dry weight and 0.53 mg ARA /g dry weight.
  • lipid product obtainable from Gammaridae produced according to the invention can be used as a feed ingredient for aquaculture purposes such as for salmon farming. It can also be used as a nutrient supplement, health product or pharmaceutical composition for humans or animals.
  • the produced lipids according to the present invention can be used as a replacement for lipids from other known marine sources such as farmed or wild caught fish, krill, copepods, marine microalgae etc.
  • Oleic acid is found as a monounsaturated fatty acid but only in an amount of 10 % of the total fatty acid (see Table 3).
  • the only polyunsaturated fatty acid present in coconut is linoleic acid.
  • the content of linoleic acid is reposted as being as low as only as 2.4 % (Table 3) of the total fatty acids.
  • Wilson et al (2005) even reported only 1.8 % as linoleic acid in coconut oil. This amount can be considered as insignificant from a biomass balance view.
  • DHA Docosahexaenoic acid (22:6n-3)
  • Gammaridae Amphipods belonging to the family of Gammaridae including species such as Gammarus oceanicus, Gammarus lawrencianus, Gammarus locusta, Gammarus lacustris, Gammarus duebeni, Gammarus obstusatus, Gammarus finnmarchicus, Gammarus stoerensis, Gammarus tigrinus, Gammarus setosus, Gammarus wilkitzkii, Gammarus sensu, or Gammarus salinus.
  • species such as Gammarus oceanicus, Gammarus lawrencianus, Gammarus locusta, Gammarus lacustris, Gammarus duebeni, Gammarus obstusatus, Gammarus finnmarchicus, Gammarus stoerensis, Gammarus tigrinus, Gammarus setosus, Gammarus wilkitzkii, Gammarus sensu, or Gammarus salinus.
  • HUFA Highly unsaturated fatty acids such as DHA, EPA and ARA
  • NFAL Lipids that are not fatty acids
  • Polyunsaturated fatty acids Fatty acid having more than one double bond in the molecule. Production: The term production refers to the intensive cultivation of
  • Marine lipid material Lipid material comprising highly unsaturated fatty acids especially chosen from the group of DHA, EPA and ARA.
  • Non-marine lipid material Lipid material not comprising the highly unsaturated fatty acids DHA, EPA and ARA. Depending on the source the material may comprise to a very low degree other highly unsaturated fatty acids such as C22:5n3.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Nutrition Science (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Insects & Arthropods (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Birds (AREA)
  • Fodder In General (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)

Abstract

Cette invention concerne un procédé d'obtention d'acides gras fortement insaturés à partir de gammaridés. Elle concerne également une denrée alimentaire pour gammaridés dont les lipides sont tirés d'un matériau lipidique non marin. L'invention concerne également différentes utilisations de cette composition alimentaire pour la production de gammaridés ainsi qu'une méthode de production de gammaridés. Elle concerne enfin un produit lipidique dérivé tiré de gammaridés et son utilisation.
PCT/NO2010/000128 2009-04-06 2010-04-06 Composition alimentaire et méthode d'alimentation de gammaridés WO2010117281A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP10719657A EP2416667A1 (fr) 2009-04-06 2010-04-06 Composition alimentaire et méthode d'alimentation de gammaridés

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
NO20091397 2009-04-06
NO20091397A NO20091397L (no) 2009-04-06 2009-04-06 Fôrsammensetning for produksjon av Gammaridae

Publications (1)

Publication Number Publication Date
WO2010117281A1 true WO2010117281A1 (fr) 2010-10-14

Family

ID=42269580

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/NO2010/000128 WO2010117281A1 (fr) 2009-04-06 2010-04-06 Composition alimentaire et méthode d'alimentation de gammaridés

Country Status (3)

Country Link
EP (1) EP2416667A1 (fr)
NO (1) NO20091397L (fr)
WO (1) WO2010117281A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103637016A (zh) * 2013-12-17 2014-03-19 广西博士海意信息科技有限公司 一种公园景观观赏鱼饲料及其制备方法
ES2567955A1 (es) * 2015-10-28 2016-04-26 Universitat D'alacant / Universidad De Alicante Procedimiento para la obtención de un producto marino a base de anfípodos

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6638561B1 (en) * 1998-06-17 2003-10-28 Dsm N.V. Microbial arachidonic acid (ARA) for use in marine feed
WO2007056823A1 (fr) * 2005-11-18 2007-05-24 Commonwealth Scientific And Industrial Research Organisation Aliments pour aquaculture comprenant des aliments à base d'acide stéaridonique pour aquaculture

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5215767A (en) * 1991-03-16 1993-06-01 Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo Additive for aquaculture feed for fish and shellfish and aquaculture feed for fish and shellfish which contains same
GB2437909A (en) * 2006-05-12 2007-11-14 Advanced Bionutrition Inc Animal feed comprising docosahexaenois acid from a microbial source

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6638561B1 (en) * 1998-06-17 2003-10-28 Dsm N.V. Microbial arachidonic acid (ARA) for use in marine feed
WO2007056823A1 (fr) * 2005-11-18 2007-05-24 Commonwealth Scientific And Industrial Research Organisation Aliments pour aquaculture comprenant des aliments à base d'acide stéaridonique pour aquaculture

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
BLIGH, E. G.DYER, W. J.: "A rapid method of total lipid extraction and purification", CAN. J. BIOCHEM. PHYSIOL., vol. 37, 1959, pages 911 - 917, XP000998224
KOLANOWSKI W, STOLYHWO A, GRABOWSKI M: "Fatty acid composition of selected fresh water Gammarids (Amphipoda, Crustacea): a potentially innovative source of omega-3 LC PUFA", J. AM. OIL. CHEM. SOC., vol. 84, 11 August 2007 (2007-08-11), pages 827 - 833, XP002589205 *
MAAZOUZI ET AL: "Fatty acid composition of the amphipod Dikerogammarus villosus: Feeding strategies and trophic links", COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY. PART A, MOLECULAR AND INTEGRATIVE PHYSIOLOGY, ELSEVIER SCIENCE, NEW YORK, NY, US LNKD- DOI:10.1016/J.CBPA.2007.02.010, vol. 147, no. 4, 1 June 2007 (2007-06-01), pages 868 - 875, XP022101994, ISSN: 1095-6433 *
METCALFE, L. D.SCHIMTZ, A. A.PELKA, J. R.: "Rapid preparation of fatty acids esters from lipids for gas chromatography", ANAL. CHEM., vol. 38, 1966, pages 514 - 515
NAGARAJU, A.BELUR, L. R.: "Rats fed blended oils containing coconut oil with groundnut oil or olive oil showed an enhanced activity of hepatic antioxidant enzymes and a reduction in LDL oxidation", FOOD CHEMISTRY, vol. 108, 2008, pages 950 - 957, XP022461501
OPSAHL-FERSTAD H-G ET AL: "Biotechnological approaches to modify rapeseed oil composition for application in aquaculture", PLANT SCIENCE, ELSEVIER IRELAND LTD, IE LNKD- DOI:10.1016/S0168-9452(03)00345-5, vol. 165, 1 January 2003 (2003-01-01), pages 349 - 357, XP003013213, ISSN: 0168-9452 *
RAINUZZO, J. R.REITAN, K. I.JORGENSEN, L.: "Comparative study on the fatty acid and lipid composition of four marine fish larvae", COMP. BIOCHEM. PHYSIOL., vol. 103B, 1992, pages 21 - 26, XP023531770, DOI: doi:10.1016/0305-0491(92)90407-I
SAITO H ET AL: "High levels of n-3 polyunsaturated fatty acids in Euphausia pacifica and its role as a source of docosahexaenoic and icosapentaenoic acids for higher trophic levels", MARINE CHEMISTRY, ELSEVIER SCIENCE B.V., AMSTERDAM, NL LNKD- DOI:10.1016/S0304-4203(02)00005-1, vol. 78, 1 January 2002 (2002-01-01), pages 9 - 28, XP003013221, ISSN: 0304-4203 *
See also references of EP2416667A1 *
WILSON, T. A.NICOLOSI, R. J.KOTYLA, T.SUNDRAM, K.KRITCHEVSKY, D.: "Different palm oil preparations reduce plasma cholesterol concentrations and aortic cholesterol accumulation compared to coconut oil in hypercholesterolemic hamsters", JOURNAL OF NUTRITIONAL BIOCHEMISTRY, vol. 16, 2005, pages 633 - 640, XP005081593, DOI: doi:10.1016/j.jnutbio.2005.03.007

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103637016A (zh) * 2013-12-17 2014-03-19 广西博士海意信息科技有限公司 一种公园景观观赏鱼饲料及其制备方法
ES2567955A1 (es) * 2015-10-28 2016-04-26 Universitat D'alacant / Universidad De Alicante Procedimiento para la obtención de un producto marino a base de anfípodos
WO2017072386A1 (fr) * 2015-10-28 2017-05-04 Universidad De Alicante Procédé pour l'obtention d'un produit marin à base d'amphipodes

Also Published As

Publication number Publication date
EP2416667A1 (fr) 2012-02-15
NO20091397L (no) 2010-10-07

Similar Documents

Publication Publication Date Title
Tan et al. Bivalves as future source of sustainable natural omega-3 polyunsaturated fatty acids
Larsen et al. Health benefits of marine foods and ingredients
Peng et al. Polyunsaturated fatty acid profiles of whole body phospholipids and triacylglycerols in anadromous and landlocked Atlantic salmon (Salmo salar L.) fry
US8895767B2 (en) Obtaining fatty acids from insect larvae
Orban et al. Quality characteristics of sea bass intensively reared and from lagoon as affected by growth conditions and the aquatic environment
Robin et al. Microparticulate diets as first food for gilthead sea bream larva (Sparus aurata): study of fatty acid incorporation
WO2009102558A2 (fr) Aliments, produits et procédés d'aquaculture comprenant des acides gras bénéfiques
Linder et al. From Krill to Whale: an overview of marine fatty acids and lipid compositions
Ljubojević et al. Comparison of meat quality of tench, Tinca tinca, reared in extensive and semi‐intensive culture systems
AU2015359335B2 (en) Feed supplement material for use in aquaculture feed
US20180303129A1 (en) Aquaculture feed formulation and aquaculture product produced with same
Gosch et al. Spatial, seasonal, and within-plant variation in total fatty acid content and composition in the brown seaweeds Dictyota bartayresii and Dictyopteris australis (Dictyotales, Phaeophyceae)
Rollin et al. The effects of dietary lipid and strain difference on polyunsaturated fatty acid composition and conversion in anadromous and landlocked salmon (Salmo salar L.) parr
Al-Khalifa Production of added-value poultry meat: enrichment with n-3 polyunsaturated fatty acids
Smichi et al. Physicochemical characterization and nutritional quality of fish by-products: in vitro oils digestibility and synthesis of flavour esters.
Elkin et al. Influence of feeding stearidonic acid (18: 4n-3)-enriched soybean oil, as compared to conventional soybean oil, on tissue deposition of very long-chain omega-3 fatty acids in meat-type chickens
Pan et al. Impacts of different feeding modes on the gonadal development, total edible yield, and nutritional composition of male Chinese mitten crab (Eriocheir sinensis)
Hossain et al. High DHA algae meal as cost-effective alternative to high DHA fish oil in finisher feed for sobaity sea bream (Sparidentex hasta)
Mulvaney et al. Recovery of omega-3 profiles of cultivated abalone by dietary macroalgae supplementation
Moran et al. Broiler tissue enrichment with docosahexaenoic acid (DHA) through dietary supplementation with Aurantiochytrium limacinum algae
Sun et al. FA composition of the oil extracted from farmed Atlantic salmon (Salmo salar L.) viscera
Nath et al. Fatty acid compositions of four edible fishes of Hooghly Estuary, West Bengal, India
Begum et al. Fatty acid composition of hilsa (Tenualosa ilisha) fish muscle from different locations in Bangladesh.
WO2010117281A1 (fr) Composition alimentaire et méthode d'alimentation de gammaridés
Demets et al. Traditional and novel sources of long-chain omega-3 fatty acids

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 10719657

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

REEP Request for entry into the european phase

Ref document number: 2010719657

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2010719657

Country of ref document: EP