WO2010115074A1 - Procédés de purification pour composés bio-organiques - Google Patents
Procédés de purification pour composés bio-organiques Download PDFInfo
- Publication number
- WO2010115074A1 WO2010115074A1 PCT/US2010/029740 US2010029740W WO2010115074A1 WO 2010115074 A1 WO2010115074 A1 WO 2010115074A1 US 2010029740 W US2010029740 W US 2010029740W WO 2010115074 A1 WO2010115074 A1 WO 2010115074A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- composition
- bio
- organic
- host cells
- crude
- Prior art date
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C7/00—Purification; Separation; Use of additives
- C07C7/10—Purification; Separation; Use of additives by extraction, i.e. purification or separation of liquid hydrocarbons with the aid of liquids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
Definitions
- Figure 6 is a plot of total acid number (TAN) as a function of time for a crude bio-organic composition that was obtained by liquid/liquid separation at an acidic pH (produced by farnesene producing yeast strain B).
- the amount of calcium hydroxide used was 0.356 % by weight.
- the clarifying step occurs by sedimentation followed by decantation. In still other embodiments, the clarifying step occurs by filtration. In certain embodiments, the clarifying step occurs by centrifugation. In certain other embodiments, the clarifying step occurs in a continuous disk stack nozzle centrifuge.
- FIG. 2 shows the separation disc covered with emulsion. When enough emulsion forms, then the centrifuge becomes ineffective in separating the bio-organic compound from the aqueous phase.
- a concentrated bio-organic composition was obtained as described by
- This example describes a dose response of the total acid number as a function of the amount of calcium hydroxide of the crude bio-organic composition derived from yeast strain B.
- TFF Process tangential flow filtration
- approximately 200 liters of clarified broth was used.
- the clarified broth was processed in series through 4 Industrial TFF cartridges (316L Stainless Steel, 1.5" diameter tube, 0.1 micrometer pore size, 0.35m 2 each).
- the purpose of the TFF processing is to remove water and aqueous components, leaving behind a concentrated clarified broth.
- the initial processing conditions for the TFF were l ⁇ psig inlet pressure, 5psig outlet pressure, and 880mL/min of permeate flux (38L/m 2 /hr). Time course samples of permeate and retentate streams were obtained. Throughout the course of processing, the permeate flux gradually dropped off due to accumulation of solids in the cartridges and increased viscosity of the retentate.
- Approximately 14 liters of concentrated clarified broth was recovered from the
Abstract
La présente invention concerne des procédés et des systèmes de purification de composés bio-organiques. Dans certains modes de réalisation, ce procédé de purification comprend une étape consistant à rendre neutre le pH de la composition. Dans d'autres modes de réalisation, le procédé de purification comprend, éventuellement, une étape de neutralisation de la composition visant à ramener l'indice d'acide à moins d'environ 0,5 mg KOH/g de composé bio-organique.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
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US16616709P | 2009-04-02 | 2009-04-02 | |
US61/166,167 | 2009-04-02 |
Publications (2)
Publication Number | Publication Date |
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WO2010115074A1 true WO2010115074A1 (fr) | 2010-10-07 |
WO2010115074A8 WO2010115074A8 (fr) | 2011-07-14 |
Family
ID=42358697
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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PCT/US2010/029740 WO2010115074A1 (fr) | 2009-04-02 | 2010-04-02 | Procédés de purification pour composés bio-organiques |
Country Status (1)
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WO (1) | WO2010115074A1 (fr) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2019030073A1 (fr) * | 2017-08-07 | 2019-02-14 | Total Raffinage Chimie | Procédé de récupération d'isoprénoïdes produits par des microorganismes |
WO2020006251A1 (fr) * | 2018-06-29 | 2020-01-02 | Amyris, Inc. | Procédés pour récupérer des composés isoprénoïdes non miscibles dans l'eau à partir d'une biomasse microbienne |
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WO2007139924A2 (fr) | 2006-05-26 | 2007-12-06 | Amyris Biotechnologies, Inc. | Dispositif de fabrication de composés bio-organiques |
WO2007140339A2 (fr) | 2006-05-26 | 2007-12-06 | Amyris Biotechnologies, Inc. | Production d'isoprénoïdes |
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WO2008133658A2 (fr) | 2006-11-21 | 2008-11-06 | Amyris Biotechnologies, Inc. | Compositions de carburant pour aviation et leurs procédés de fabrication et d'utilisation |
WO2008140492A2 (fr) | 2006-11-21 | 2008-11-20 | Amyris Biotechnologies, Inc. | Compositions de carburéacteur et leurs procédés de fabrication et d'utilisation |
WO2008151149A2 (fr) | 2007-06-01 | 2008-12-11 | Solazyme, Inc. | Production d'huile dans des micro-organismes |
WO2009014636A2 (fr) | 2007-07-20 | 2009-01-29 | Amyris Biotechnologies, Inc. | Compositions de carburant comprenant du tétraméthylcyclohexane |
-
2010
- 2010-04-02 WO PCT/US2010/029740 patent/WO2010115074A1/fr active Application Filing
Patent Citations (11)
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WO2007139924A2 (fr) | 2006-05-26 | 2007-12-06 | Amyris Biotechnologies, Inc. | Dispositif de fabrication de composés bio-organiques |
WO2007140339A2 (fr) | 2006-05-26 | 2007-12-06 | Amyris Biotechnologies, Inc. | Production d'isoprénoïdes |
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US7399323B2 (en) | 2006-10-10 | 2008-07-15 | Amyris Biotechnologies, Inc. | Fuel compositions comprising farnesane and farnesane derivatives and method of making and using same |
WO2008133658A2 (fr) | 2006-11-21 | 2008-11-06 | Amyris Biotechnologies, Inc. | Compositions de carburant pour aviation et leurs procédés de fabrication et d'utilisation |
WO2008140492A2 (fr) | 2006-11-21 | 2008-11-20 | Amyris Biotechnologies, Inc. | Compositions de carburéacteur et leurs procédés de fabrication et d'utilisation |
WO2008113041A2 (fr) | 2007-03-14 | 2008-09-18 | Ls9, Inc. | Procédé de production d'hydrocarbures de bas poids moléculaire à partir de ressources renouvelables |
WO2008151149A2 (fr) | 2007-06-01 | 2008-12-11 | Solazyme, Inc. | Production d'huile dans des micro-organismes |
US20090047721A1 (en) | 2007-06-01 | 2009-02-19 | Solazyme, Inc. | Renewable Diesel and Jet Fuel from Microbial Sources |
WO2009014636A2 (fr) | 2007-07-20 | 2009-01-29 | Amyris Biotechnologies, Inc. | Compositions de carburant comprenant du tétraméthylcyclohexane |
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AMALVY ET AL., CHEM. COMMUN., 2003, pages 1826 - 182 |
GALLUP ET AL., ENERGY FUELS, vol. 21, no. 3, 2007, pages 1741 - 1759 |
GEORGE ALTHER, WATER ENGINEERING & MANAGEMENT, 2001, pages 27 - 29 |
RO DAE-KYUN ET AL: "Production of the antimalarial drug precursor artemisinic acid in engineered yeast", NATURE, NATURE PUBLISHING GROUP, LONDON, GB LNKD- DOI:10.1038/NATURE04640, vol. 440, 13 April 2006 (2006-04-13), pages 940 - 943, XP002510909, ISSN: 0028-0836 * |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2019030073A1 (fr) * | 2017-08-07 | 2019-02-14 | Total Raffinage Chimie | Procédé de récupération d'isoprénoïdes produits par des microorganismes |
US11103808B2 (en) | 2017-08-07 | 2021-08-31 | Amyris, Inc. | Process for recovering isoprenoids produced by microorganisms |
WO2020006251A1 (fr) * | 2018-06-29 | 2020-01-02 | Amyris, Inc. | Procédés pour récupérer des composés isoprénoïdes non miscibles dans l'eau à partir d'une biomasse microbienne |
CN112771169A (zh) * | 2018-06-29 | 2021-05-07 | 阿迈瑞斯公司 | 从微生物生物质回收水不混溶性类异戊二烯化合物的方法 |
JP2021529512A (ja) * | 2018-06-29 | 2021-11-04 | アミリス, インコーポレイテッド | 微生物バイオマスから不水溶性イソプレノイド化合物を回収するための方法 |
US11312976B2 (en) | 2018-06-29 | 2022-04-26 | Amyris, Inc. | Methods for recovering water-immiscible isoprenoid compounds from microbial biomass |
Also Published As
Publication number | Publication date |
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WO2010115074A8 (fr) | 2011-07-14 |
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