WO2010106428A3 - Microfluidic system - Google Patents

Microfluidic system Download PDF

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Publication number
WO2010106428A3
WO2010106428A3 PCT/IB2010/000585 IB2010000585W WO2010106428A3 WO 2010106428 A3 WO2010106428 A3 WO 2010106428A3 IB 2010000585 W IB2010000585 W IB 2010000585W WO 2010106428 A3 WO2010106428 A3 WO 2010106428A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
valves
downstream
sample
given type
Prior art date
Application number
PCT/IB2010/000585
Other languages
French (fr)
Other versions
WO2010106428A2 (en
Inventor
Gianni Medoro
Gerardo Perozziello
Alex Calanca
Giuseppina Simone
Nicolò Manaresi
Original Assignee
Silicon Biosystems S.P.A.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Silicon Biosystems S.P.A. filed Critical Silicon Biosystems S.P.A.
Priority to DK10716034.3T priority Critical patent/DK2408560T3/en
Priority to SI201032084T priority patent/SI2408560T1/en
Priority to PL10716034T priority patent/PL2408560T3/en
Priority to EP10716034.3A priority patent/EP2408560B1/en
Priority to ES10716034T priority patent/ES2885001T3/en
Publication of WO2010106428A2 publication Critical patent/WO2010106428A2/en
Publication of WO2010106428A3 publication Critical patent/WO2010106428A3/en

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/02—Separators
    • B03C5/022—Non-uniform field separators
    • B03C5/026—Non-uniform field separators using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0621—Control of the sequence of chambers filled or emptied
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0631—Purification arrangements, e.g. solid phase extraction [SPE]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652—Sorting or classification of particles or molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087—Multiple sequential chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/14—Means for pressure control
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1822—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using Peltier elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0433—Moving fluids with specific forces or mechanical means specific forces vibrational forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B01L2400/0655—Valves, specific forms thereof with moving parts pinch valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C2201/00—Details of magnetic or electrostatic separation
    • B03C2201/02—Electro-statically separating liquids from liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C2201/00—Details of magnetic or electrostatic separation
    • B03C2201/26—Details of magnetic or electrostatic separation for use in medical applications

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Fluid Mechanics (AREA)
  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Microelectronics & Electronic Packaging (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

A microfluidic system (1) for the isolation of cells (C1) of at least one given type from a sample; the system (1) comprises a separation unit (3), - for transferring at least part of the cells (Cl) of the given type from a main chamber (4) to a recovery chamber (5) in a substantially selective way with respect to further cells (C2) of the sample; two valves (9, 10) are set upstream and downstream of the main chamber (4); two valves (11, 12) are set upstream and downstream of the recovery chamber (5); a control assembly (23) is designed to govern the aforementioned valves (9, 10, 11, 12); the system (1) proposed enables isolation of the cells with a high degree of reproducibility and precision.
PCT/IB2010/000585 2009-03-17 2010-03-17 Microfluidic system WO2010106428A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
DK10716034.3T DK2408560T3 (en) 2009-03-17 2010-03-17 Microfluidic system for isolating particles
SI201032084T SI2408560T1 (en) 2009-03-17 2010-03-17 Microfluidic system for the isolation of particles
PL10716034T PL2408560T3 (en) 2009-03-17 2010-03-17 Microfluidic system for the isolation of particles
EP10716034.3A EP2408560B1 (en) 2009-03-17 2010-03-17 Microfluidic system for the isolation of particles
ES10716034T ES2885001T3 (en) 2009-03-17 2010-03-17 Microfluidic system for particle isolation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ITBO2009A000154 2009-03-17
IT000154A ITBO20090154A1 (en) 2009-03-17 2009-03-17 MICROFLUID SYSTEM

Publications (2)

Publication Number Publication Date
WO2010106428A2 WO2010106428A2 (en) 2010-09-23
WO2010106428A3 true WO2010106428A3 (en) 2010-12-23

Family

ID=41303338

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2010/000585 WO2010106428A2 (en) 2009-03-17 2010-03-17 Microfluidic system

Country Status (9)

Country Link
EP (1) EP2408560B1 (en)
DK (1) DK2408560T3 (en)
ES (1) ES2885001T3 (en)
HU (1) HUE056110T2 (en)
IT (1) ITBO20090154A1 (en)
PL (1) PL2408560T3 (en)
PT (1) PT2408560T (en)
SI (1) SI2408560T1 (en)
WO (1) WO2010106428A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1397820B1 (en) 2009-12-17 2013-02-04 Silicon Biosystems Spa MICROFLUID SYSTEM
DE102012019819A1 (en) * 2012-10-10 2014-04-10 Claus Fütterer Method and device for controlling the pressure in a micro- or mesofluidic channel
IT201600104601A1 (en) 2016-10-18 2018-04-18 Menarini Silicon Biosystems Spa MICROFLUID SYSTEM
KR102637616B1 (en) * 2016-10-18 2024-02-16 메나리니 실리콘 바이오시스템스 에스.피.에이. Microfluidic devices, microfluidic systems and particle separation methods
IT201700105948A1 (en) 2017-09-21 2019-03-21 Menarini Silicon Biosystems Spa METHOD AND MICROFLUID SYSTEM FOR RECOVERY OF PARTICLES

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030044832A1 (en) * 1996-09-04 2003-03-06 Scandinavian Micro Biodevices A/S Microflow system for particle separation and analysis
US7081192B1 (en) * 2000-08-08 2006-07-25 Aviva Biosciences Corporation Methods for manipulating moieties in microfluidic systems
US20060177815A1 (en) * 2004-11-29 2006-08-10 The Regents Of The University Of California Dielectrophoretic particle sorter
US20080261295A1 (en) * 2007-04-20 2008-10-23 William Frank Butler Cell Sorting System and Methods

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1179585B1 (en) * 1997-12-24 2008-07-09 Cepheid Device and method for lysis
US6387632B2 (en) * 1998-06-11 2002-05-14 Hitachi, Ltd. Polynucleotide separation method and apparatus therefor
DE60022025T2 (en) * 1999-05-28 2006-06-29 Cepheid, Sunnyvale APPENDIX FOR BREAKING CELLS
CN1370278A (en) * 1999-08-11 2002-09-18 旭化成株式会社 Analyzing cartridge and liquid feed control device
US20030073110A1 (en) * 2001-07-03 2003-04-17 Masaharu Aritomi Method for isolating nucleic acid and a cartridge for chemical reaction and for nucleic acid isolation
WO2004000721A2 (en) * 2002-06-24 2003-12-31 Fluidigm Corporation Recirculating fluidic network and methods for using the same
WO2004048564A1 (en) * 2002-11-28 2004-06-10 Arkray Inc. Device for pretreating specimen
US7476360B2 (en) * 2003-12-09 2009-01-13 Genefluidics, Inc. Cartridge for use with electrochemical sensor
DE102004050575B3 (en) * 2004-10-15 2006-01-05 Siemens Ag Method for the combined isolation of magnetic beads from a liquid sample and subsequent thermal cycling for the PCR and associated assembly
CN101004423B (en) * 2006-01-19 2011-12-28 博奥生物有限公司 Card, kit system in use for analyzing sample of liquid
KR100813254B1 (en) * 2006-05-29 2008-03-13 삼성전자주식회사 An apparatus for separating a polarizable analyte using dielectrophoresis and a method of separating a polarizable analyte using the same

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030044832A1 (en) * 1996-09-04 2003-03-06 Scandinavian Micro Biodevices A/S Microflow system for particle separation and analysis
US7081192B1 (en) * 2000-08-08 2006-07-25 Aviva Biosciences Corporation Methods for manipulating moieties in microfluidic systems
US20060177815A1 (en) * 2004-11-29 2006-08-10 The Regents Of The University Of California Dielectrophoretic particle sorter
US20080261295A1 (en) * 2007-04-20 2008-10-23 William Frank Butler Cell Sorting System and Methods

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
FIEDLER S ET AL: "DIELECTROPHORETIC SORTING OF PARTICLES AND CELLS IN A MICROSYSTEM", ANALYTICAL CHEMISTRY, AMERICAN CHEMICAL SOCIETY, US LNKD- DOI:10.1021/AC971063B, vol. 70, no. 9, 1 May 1998 (1998-05-01), pages 1909 - 1915, XP000755524, ISSN: 0003-2700 *

Also Published As

Publication number Publication date
PL2408560T3 (en) 2021-11-08
SI2408560T1 (en) 2021-11-30
ITBO20090154A1 (en) 2010-09-18
WO2010106428A2 (en) 2010-09-23
PT2408560T (en) 2021-07-30
DK2408560T3 (en) 2021-07-26
EP2408560A2 (en) 2012-01-25
EP2408560B1 (en) 2021-06-23
HUE056110T2 (en) 2022-01-28
ES2885001T3 (en) 2021-12-13

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