WO2010104357A2 - 환자맞춤형 줄기세포치료를 위한 세포투과성 역분화 전사인자를 이용한 유도만능줄기세포 확립 - Google Patents

환자맞춤형 줄기세포치료를 위한 세포투과성 역분화 전사인자를 이용한 유도만능줄기세포 확립 Download PDF

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WO2010104357A2
WO2010104357A2 PCT/KR2010/001559 KR2010001559W WO2010104357A2 WO 2010104357 A2 WO2010104357 A2 WO 2010104357A2 KR 2010001559 W KR2010001559 W KR 2010001559W WO 2010104357 A2 WO2010104357 A2 WO 2010104357A2
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cell
stem cell
reprogramming
establishment
recombinant protein
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PCT/KR2010/001559
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English (en)
French (fr)
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WO2010104357A3 (ko
WO2010104357A4 (ko
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조대웅
임정희
김정은
정수영
정인희
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주식회사 프로셀제약
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Priority to EP10751048A priority Critical patent/EP2407488A4/en
Priority to CN2010800210349A priority patent/CN102439045A/zh
Priority to US13/263,955 priority patent/US20120095188A1/en
Publication of WO2010104357A2 publication Critical patent/WO2010104357A2/ko
Publication of WO2010104357A3 publication Critical patent/WO2010104357A3/ko
Publication of WO2010104357A4 publication Critical patent/WO2010104357A4/ko

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/10—Fusion polypeptide containing a localisation/targetting motif containing a tag for extracellular membrane crossing, e.g. TAT or VP22

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

본발명은 역분화 유도전사인자 (Reprogramming Transcription Factor)에 거대분자 전송 도메인 (macromolecule transduction domain, MTD)이 융합되어 세포투과성을 갖는 역분화 전사인자 재조합 단백질, 상기 역분화 전사인자 재조합 단백질을 코딩하는 폴리뉴클레오티드, 상기 세포투과성 역분화 전사인자 재조합 단백질의 발현벡터에 관한 것이다. 상기 세포투과성 역분화 전사인자 재조합 단백질이 체세포에 처리되면 체세포의 줄기세포 특이적 유전자의 리프로그래밍(Reprogramming)을 유도하여 외형적(morphology), 유전적(genetic)으로 배아줄기세포와 유사한 특징을 가지고 있는 유도만능줄기세포 (iPS cell)를 확립하는데 유용하게 사용될 수 있다.
PCT/KR2010/001559 2009-03-12 2010-03-12 환자맞춤형 줄기세포치료를 위한 세포투과성 역분화 전사인자를 이용한 유도만능줄기세포 확립 WO2010104357A2 (ko)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP10751048A EP2407488A4 (en) 2009-03-12 2010-03-12 DETERMINATION OF INDUCED PLURIPOTENT STEM CELLS BASED ON A CELL PERMEABLIC REPOGRAMMING TRANSCRIPTION FACTOR FOR PERSONALIZED STEM CELL THERAPY
CN2010800210349A CN102439045A (zh) 2009-03-12 2010-03-12 利用可穿透细胞的重编程转录因子建立诱导性多能干细胞用于个性化干细胞疗法
US13/263,955 US20120095188A1 (en) 2009-03-12 2010-03-12 Establishment of induced pluripotent stem cell using cell-permeable reprogramming transcription factor for customized stem cell therapy

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US15946109P 2009-03-12 2009-03-12
US61/159,461 2009-03-12

Publications (3)

Publication Number Publication Date
WO2010104357A2 true WO2010104357A2 (ko) 2010-09-16
WO2010104357A3 WO2010104357A3 (ko) 2011-03-03
WO2010104357A4 WO2010104357A4 (ko) 2011-04-21

Family

ID=42728969

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2010/001559 WO2010104357A2 (ko) 2009-03-12 2010-03-12 환자맞춤형 줄기세포치료를 위한 세포투과성 역분화 전사인자를 이용한 유도만능줄기세포 확립

Country Status (5)

Country Link
US (1) US20120095188A1 (ko)
EP (1) EP2407488A4 (ko)
KR (1) KR20120034595A (ko)
CN (1) CN102439045A (ko)
WO (1) WO2010104357A2 (ko)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101529634B1 (ko) * 2013-08-28 2015-06-30 서울대학교산학협력단 역분화 유도를 위한 세포투과성 융합 단백질 및 그 용도
WO2017026776A1 (en) 2015-08-10 2017-02-16 Cellivery Therapeutics, Inc. Improved cell-permeable reprogramming factor (icp-rf) recombinant protein and use thereof
CN118109518B (zh) * 2024-04-30 2024-06-21 南京鸿明生物科技有限公司 基于mRNA-LNP的诱导多能性干细胞的重编程方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2676797A1 (en) 2007-01-29 2008-08-07 Dae Woong Jo Novel macromolecule transduction domains and methods for identification and uses thereof

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2132225A4 (en) * 2007-02-27 2010-06-09 Procell Therapeutics Inc COMBINED USE OF NANOG AND OCT4 PERMEABLE TO CELLS TO INCREASE SELF-RENEWAL AND DELETE DIFFERENTIATION OF STEM CELLS
KR100887266B1 (ko) * 2007-09-04 2009-03-06 주식회사 프로셀제약 세포투과성 ｐ１８ 재조합 단백질, 이를 코딩하는폴리뉴클레오티드 및 이를 유효성분으로 함유하는 항암조성물

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2676797A1 (en) 2007-01-29 2008-08-07 Dae Woong Jo Novel macromolecule transduction domains and methods for identification and uses thereof
AU2008211854A1 (en) 2007-01-29 2008-08-07 Procell Therapeutics Inc. Novel macromolecule transduction domains and methods for identification and uses thereof

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
DEUTSCHER, M.: "Guide to Protein Purification Methods Enzymology", vol. 182, 1990, ACADEMIC PRESS. INC.
MANIATIS ET AL.: "Molecular Cloning: A Laboratory Manual", 1982, COLD SPRING HARBOR LABORATORY
SAMBROOK ET AL.: "Molecular Cloning: A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
See also references of EP2407488A4
YAMANAKA, CELL, vol. 126, 2006, pages 633 - 678
YU J, SCIENCE, vol. 318, no. 5858, 2007, pages 1917 - 20

Also Published As

Publication number Publication date
WO2010104357A3 (ko) 2011-03-03
US20120095188A1 (en) 2012-04-19
CN102439045A (zh) 2012-05-02
EP2407488A4 (en) 2013-03-13
EP2407488A2 (en) 2012-01-18
WO2010104357A4 (ko) 2011-04-21
KR20120034595A (ko) 2012-04-12

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