WO2010102632A3 - Rna purification method - Google Patents

Rna purification method Download PDF

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Publication number
WO2010102632A3
WO2010102632A3 PCT/DK2010/050056 DK2010050056W WO2010102632A3 WO 2010102632 A3 WO2010102632 A3 WO 2010102632A3 DK 2010050056 W DK2010050056 W DK 2010050056W WO 2010102632 A3 WO2010102632 A3 WO 2010102632A3
Authority
WO
WIPO (PCT)
Prior art keywords
purification method
rna purification
sample
collection media
rna
Prior art date
Application number
PCT/DK2010/050056
Other languages
French (fr)
Other versions
WO2010102632A2 (en
Inventor
Maria Rossing
Lennart Friis-Hansen
Original Assignee
Rigshospitalet
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rigshospitalet filed Critical Rigshospitalet
Publication of WO2010102632A2 publication Critical patent/WO2010102632A2/en
Publication of WO2010102632A3 publication Critical patent/WO2010102632A3/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D63/00—Apparatus in general for separation processes using semi-permeable membranes
    • B01D63/08—Flat membrane modules
    • B01D63/087—Single membrane modules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D71/00—Semi-permeable membranes for separation processes or apparatus characterised by the material; Manufacturing processes specially adapted therefor
    • B01D71/06—Organic material
    • B01D71/30—Polyalkenyl halides
    • B01D71/32—Polyalkenyl halides containing fluorine atoms
    • B01D71/34—Polyvinylidene fluoride
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082—Test tubes per se
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2313/00—Details relating to membrane modules or apparatus
    • B01D2313/20—Specific housing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2313/00—Details relating to membrane modules or apparatus
    • B01D2313/20—Specific housing
    • B01D2313/206—Specific housing characterised by the material
    • B01D2313/2061—Organic, e.g. polymeric material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/042—Caps; Plugs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/043—Hinged closures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Clinical Laboratory Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The present invention provides a device comprising a detachable filter section and at least one tube section. Also provided is a method for collecting a sample in a non-toxic easy-to-use collection media and efficiently extracting said sample from said collection media thereby obtaining sufficient quantity and quality of RNA, DNA or protein from said samples, such as single in vivo fine-needle aspirates.
PCT/DK2010/050056 2009-03-12 2010-03-11 Improved rna purification method WO2010102632A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200900343 2009-03-12
DKPA200900343 2009-03-12

Publications (2)

Publication Number Publication Date
WO2010102632A2 WO2010102632A2 (en) 2010-09-16
WO2010102632A3 true WO2010102632A3 (en) 2010-11-25

Family

ID=42307746

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2010/050056 WO2010102632A2 (en) 2009-03-12 2010-03-11 Improved rna purification method

Country Status (1)

Country Link
WO (1) WO2010102632A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011079846A2 (en) 2009-12-30 2011-07-07 Rigshospitalet Mrna classification of thyroid follicular neoplasia
CN107497180B (en) * 2017-10-10 2019-11-12 南京南欣医药技术研究院有限公司 A kind of preparation method of biodegradation type High dust holding amount air filting material
KR102639140B1 (en) * 2020-08-14 2024-02-20 연세대학교 산학협력단 Method for biopsy and apparatus for biopsy

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0298513A1 (en) * 1987-07-08 1989-01-11 Szabados, Andreas, Dr.med. Filter unit, particularly for medical samples
US4863610A (en) * 1987-06-11 1989-09-05 Campbell Brian D Scraping, collecting & eluting apparatus for thin layer chromatography and method for its use
WO1992016294A1 (en) * 1991-03-19 1992-10-01 Minnesota Mining And Manufacturing Company A device and a method for separating liquid samples
US5873967A (en) * 1995-05-19 1999-02-23 Millipore Corporation Method for making a vaccum filter device
US20070238118A1 (en) * 2006-04-06 2007-10-11 Ambion, Inc. Methods and kits for sequentially isolating rna and genomic dna from cells
WO2008002370A2 (en) * 2006-06-28 2008-01-03 Jms Company, Ltd. Double membrane transducer protector

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4863610A (en) * 1987-06-11 1989-09-05 Campbell Brian D Scraping, collecting & eluting apparatus for thin layer chromatography and method for its use
EP0298513A1 (en) * 1987-07-08 1989-01-11 Szabados, Andreas, Dr.med. Filter unit, particularly for medical samples
WO1992016294A1 (en) * 1991-03-19 1992-10-01 Minnesota Mining And Manufacturing Company A device and a method for separating liquid samples
US5873967A (en) * 1995-05-19 1999-02-23 Millipore Corporation Method for making a vaccum filter device
US20070238118A1 (en) * 2006-04-06 2007-10-11 Ambion, Inc. Methods and kits for sequentially isolating rna and genomic dna from cells
WO2008002370A2 (en) * 2006-06-28 2008-01-03 Jms Company, Ltd. Double membrane transducer protector

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ANONYMOUS: "Centricon - Centrifugal filter devices - user guide", March 2005 (2005-03-01), XP002600140, Retrieved from the Internet <URL:http://kirschner.med.harvard.edu/files/protocols/Millipore_Centricons.pdf> [retrieved on 20100909] *
ANONYMOUS: "Datasheet - Technical data and operating instructions VIVASPIN 500 uL and 2 mL", September 2008 (2008-09-01), XP002600141, Retrieved from the Internet <URL:http://www.vivaproducts.com/downloads/vivaspin-2-500-data-sheet.pdf> [retrieved on 20100910] *

Also Published As

Publication number Publication date
WO2010102632A2 (en) 2010-09-16

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