WO2010098862A3 - Method of using an oligonucleotide microarray to detect cancer from serum nucleic acid - Google Patents

Method of using an oligonucleotide microarray to detect cancer from serum nucleic acid Download PDF

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Publication number
WO2010098862A3
WO2010098862A3 PCT/US2010/000569 US2010000569W WO2010098862A3 WO 2010098862 A3 WO2010098862 A3 WO 2010098862A3 US 2010000569 W US2010000569 W US 2010000569W WO 2010098862 A3 WO2010098862 A3 WO 2010098862A3
Authority
WO
WIPO (PCT)
Prior art keywords
probes
nucleic acid
detect cancer
oligonucleotide
microarray
Prior art date
Application number
PCT/US2010/000569
Other languages
French (fr)
Other versions
WO2010098862A2 (en
Inventor
Michael J. Lodes
Dominic Suciu
Marcelo Caraballo
Andrew Antoniewicz
Original Assignee
Combimatrix Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Combimatrix Corporation filed Critical Combimatrix Corporation
Publication of WO2010098862A2 publication Critical patent/WO2010098862A2/en
Publication of WO2010098862A3 publication Critical patent/WO2010098862A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/178—Oligonucleotides characterized by their use miRNA, siRNA or ncRNA

Abstract

Disclosed herein is a method of using an oligonucleotide microarray to detect cancer from serum micro RNA. The method provides a microarray of oligonucleotide probes. A serum miRNA sample is hybridized to the microarray of oligonucleotide probes. The oligonucleotide probes comprise complementary probes to the miRNAs in the serum miRNA sample and mismatch probes having mismatches from the complementary probes as well as control spike in probes.
PCT/US2010/000569 2009-02-24 2010-02-24 Method of using an oligonucleotide microarray to detect cancer from serum nucleic acid WO2010098862A2 (en)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US20855509P 2009-02-24 2009-02-24
US61/208,555 2009-02-24
US21610509P 2009-05-12 2009-05-12
US61/216,105 2009-05-12
US21637609P 2009-05-14 2009-05-14
US61/216,376 2009-05-14
US21718409P 2009-05-27 2009-05-27
US61/217,184 2009-05-27

Publications (2)

Publication Number Publication Date
WO2010098862A2 WO2010098862A2 (en) 2010-09-02
WO2010098862A3 true WO2010098862A3 (en) 2012-07-19

Family

ID=42666124

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2010/000569 WO2010098862A2 (en) 2009-02-24 2010-02-24 Method of using an oligonucleotide microarray to detect cancer from serum nucleic acid

Country Status (1)

Country Link
WO (1) WO2010098862A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012040500A2 (en) * 2010-09-22 2012-03-29 Hoon Dave S B Direct blood assay for detection of circulating microrna in cancer patients
EP2734642A1 (en) * 2011-07-22 2014-05-28 SCHMIEGEL, Wolff Snrna rnu2-1 as a tumor marker
US8846316B2 (en) 2012-04-30 2014-09-30 Industrial Technology Research Institute Biomarker for human liver cancer

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003016475A2 (en) * 2001-08-14 2003-02-27 The General Hospital Corporation Nucleic acid and amino acid sequences involved in pain
US20040181048A1 (en) * 2000-10-24 2004-09-16 Wang David G Identification and mapping of single nucleotide polymorphisms in the human genome
US20060160121A1 (en) * 2004-10-05 2006-07-20 Wyeth Probe arrays for detecting multiple strains of different species
US20070039076A1 (en) * 1999-07-20 2007-02-15 Boukharov Andrey A Plant genome sequence and uses thereof
US20070054278A1 (en) * 2003-11-18 2007-03-08 Applera Corporation Polymorphisms in nucleic acid molecules encoding human enzyme proteins, methods of detection and uses thereof
US20070067865A1 (en) * 2000-09-05 2007-03-22 Kovalic David K Annotated plant genes
US20070083945A1 (en) * 2000-03-10 2007-04-12 Byrum Joseph R Nucleic acid molecules and other molecules associated with plants
US20070083334A1 (en) * 2001-09-14 2007-04-12 Compugen Ltd. Methods and systems for annotating biomolecular sequences
US20070161004A1 (en) * 2004-05-28 2007-07-12 David Brown Methods and compositions involving microRNA
US20080263730A1 (en) * 1999-05-07 2008-10-23 Andersen Scott E Nucleic acid molecules and other molecules associated with plants

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080263730A1 (en) * 1999-05-07 2008-10-23 Andersen Scott E Nucleic acid molecules and other molecules associated with plants
US20070039076A1 (en) * 1999-07-20 2007-02-15 Boukharov Andrey A Plant genome sequence and uses thereof
US20070083945A1 (en) * 2000-03-10 2007-04-12 Byrum Joseph R Nucleic acid molecules and other molecules associated with plants
US20070067865A1 (en) * 2000-09-05 2007-03-22 Kovalic David K Annotated plant genes
US20040181048A1 (en) * 2000-10-24 2004-09-16 Wang David G Identification and mapping of single nucleotide polymorphisms in the human genome
WO2003016475A2 (en) * 2001-08-14 2003-02-27 The General Hospital Corporation Nucleic acid and amino acid sequences involved in pain
US20070083334A1 (en) * 2001-09-14 2007-04-12 Compugen Ltd. Methods and systems for annotating biomolecular sequences
US20070054278A1 (en) * 2003-11-18 2007-03-08 Applera Corporation Polymorphisms in nucleic acid molecules encoding human enzyme proteins, methods of detection and uses thereof
US20070161004A1 (en) * 2004-05-28 2007-07-12 David Brown Methods and compositions involving microRNA
US20060160121A1 (en) * 2004-10-05 2006-07-20 Wyeth Probe arrays for detecting multiple strains of different species

Also Published As

Publication number Publication date
WO2010098862A2 (en) 2010-09-02

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