WO2010096987A1

WO2010096987A1 - Use of patchouli alcohol in medicament preparation

Info

Publication number: WO2010096987A1
Application number: PCT/CN2009/074811
Authority: WO
Inventors: 赖小平; 苏子仁; 张奉学; 彭绍忠; 胡家敏
Original assignee: 东莞广州中医药大学中医药数理工程研究院
Priority date: 2009-02-27
Filing date: 2009-11-05
Publication date: 2010-09-02
Also published as: CN101485647A; CN101485647B

Abstract

New use of patchouli alcohol, CO₂ supercritical extracts of herba pogostemonis or patchouli oil in medicament preparation is disclosed. Patchouli alcohol, having distinct inhibiting and killing effect on influenza virus H1N1, influenza virus B and avian influenza virus H5N1, can be found in both CO₂ supercritical extracts of herba pogostemonis and patchouli oil.

Description

Use of Baiqiu Li alcohol in drug preparation

The invention relates to the use of patchouli carbon dioxide supercritical extract, patchouli oil or baicaleol in the preparation of medicaments, and belongs to the field of medicine. Background technique

Patchouli, Pogostemon cabl in (Blanco) Benth., also known as sorrel, musk, is a genus of the genus Labia, a perennial herb, used in whole herbs. It is native to subtropical regions such as Asia and the Philippines. China mainly produces Guangdong and Hainan. Its taste, tenderness, spleen, stomach, lungs. It has aroma, turbidity, appetizing and vomiting, and publishes heat-removing effects, etc. It is mostly used for wet turbidity, vomiting, vomiting, stagnation, chest tightness, cold and dampness, abdominal pain, vomiting and diarrhea, and nasal pain. The medicinal ingredient of patchouli is mainly its volatile oil, which is called patchouli oil. Its whole grass contains about 1.5% patchouli oil, and its dry product contains about 2% 2.8% of patchouli oil, and the leaf contains 4. 5 % patchouli oil. Patchouli oil promotes gastric juice secretion, enhances digestive function, and relieves gastric mucosal muscles and antibacterial effects.

The main ingredient of patchouli oil is patchoul alcohol, which is about 52% 57%. It is a tricyclic sesquiterpenoid compound with a molecular formula of C ₁₅ H ₂₆ 0 and a molecular weight of 222. 37. The chemical name is (1R-(lalpha, 4beta, 4aalpha, 6beta, 8aalpha) ) - octahydro-4, 8a, 9 9_tetramethyl_l 6- meth 1-1 (2H) -naphthol , and its structural formula is shown below:

For crystallization, melting point 55 56%, boiling point 140 ° C (1·06 kPa) 1. 0284 1. 5029, optical rotation -97. 4 ° (c = 24 chloroform). Do not dissolve in water, soluble in alcohol, ether and common organic solvents. Racemic melting point 39 40%

Up to now, people's understanding of the function and use of patchouli, patchouli oil and its main ingredient, Baiqiu Liol, has been limited to being a traditional spice, added to cosmetics or essential oils, such as European Patent EP0409089A2, which reveals a A perfume component containing ubiquinol. Patent WO20041153153A1 also discloses that patchouli oil, Baiqiuliol and its derivatives can be used to inhibit the asexual reproduction of fungi and reduce the adhesion of microorganisms, heal wounds or use in detergents, etc. The effect of the face. Chinese patent 200710176344. 8 reveals the efficacy and use of Baiqiu Li alcohol in the prevention and treatment of senile dementia. However, the efficacy and use of patchouli oil and its main ingredient, Baiqiu Li alcohol, in other aspects are rarely reported.

Influenza (Influenza) is an acute respiratory infection caused by influenza virus. It is a highly infectious and fast-transmitting disease. It is mainly transmitted by droplets in the air, or by contact.

Human influenza viruses are classified into three types: A, B, and C. Their antigenicity and virulence are highly susceptible to mutation, especially the type A virus. Therefore, influenza is highly prone to occur and is highly contagious. China is a country with frequent influenza, and three world pandemics since 1957 have originated in China. In 1917-1919, the outbreak of flu in Europe killed 20 million people and was the worst flu outbreak in history.

Avian influenza (Bird Flu or Avian Influenza), also known as avian influenza, is a syndrome caused by avian influenza virus (AIV) in avian animals from a respiratory system to the body with severe sepsis. . Avian influenza has been designated as a Class A animal disease by the World Organisation for Animal Health (0IE). It is classified as a type of animal disease in China, also known as "true chicken" or "European chicken". Among the global avian influenza patients, the highest number of cases and the highest mortality rate is the 3⁄4 avian influenza virus. At present, it has occurred in many countries and regions in the world, causing huge economic losses to the poultry industry.

Because the spread of the flu is extremely fast, the virus is highly susceptible to variability, so the flu will occur at different scales every year. According to data released by the WHO, it is estimated that there are 600 to 1.2 billion cases of influenza worldwide each year.

For decades, countries around the world have invested a lot of manpower and resources in research on anti-influenza viruses, hoping to find more effective ways to prevent and treat influenza viruses. The development of antiviral drugs is mainly aimed at preventing the adsorption of viruses, preventing the replication of viral nucleic acids, and preventing the translation of viral messenger RNA into viral proteins, neuraminidase inhibitors and other metabolic pathways. Currently developed drugs are amantadine (remantadine), ribavirin (tibavirin), λ-interferon, zanamivir (zanamivir) and osmivir (o seltamivir). Existing drugs not only have side effects, but also have drug resistance. Therefore, there is an urgent need to develop new highly effective anti-cold drugs that can effectively prevent, treat influenza, and have a safer source and lower price.

In the process of screening anti-influenza drugs, the present invention finds that the patchouli oil and the patchouli carbon dioxide supercritical extract group have obvious effects on influenza A virus, influenza B virus and avian influenza virus. Inhibition and killing effect.

To this end, one of the objects of the present invention is to provide an application of baicalol in the preparation of a medicament for treating or preventing influenza or a health care product.

The second object of the present invention is to provide a patchouli carbon dioxide supercritical extract group for the preparation or treatment of influenza drugs. Application in food or health care products.

A third object of the present invention is to provide a use of a patchouli carbon dioxide supercritical extract group for the preparation of a medicament for the treatment or prevention of influenza or a health care product.

A fourth object of the present invention is to provide an application of the pharmaceutical composition of the present invention for the preparation of a medicament for treating or preventing influenza or a health care product.

The technical scheme of the present invention is as follows: The application of the baicalin in the preparation of a medicament for treating or preventing influenza.

The application of patchouli carbon dioxide supercritical extract in the preparation of a medicament for treating or preventing influenza.

The use of patchouli oil in the preparation of a medicament for treating or preventing influenza.

The use of a composition comprising the active active ingredient, baicaleol, and a pharmaceutically acceptable carrier for the preparation of a medicament for the treatment or prevention of influenza.

The use of Baiqiu Li alcohol in the preparation of a medicament for treating or preventing influenza A caused by influenza A virus.

The use of Baiqiu Li alcohol in the preparation of a medicament for treating or preventing influenza caused by influenza B virus.

The use of Baiqiu Li alcohol in the preparation of influenza drugs caused by the treatment or prevention of avian influenza virus.

An anti-influenza drug composition comprising the active active ingredient, baicalol, and/or a pharmaceutically acceptable carrier.

The anti-influenza drug composition, the active active ingredient baqiuli alcohol raw material is derived from patchouli carbon dioxide supercritical extract, Guanghuo sesame oil or Guanghuoxiang.

The composition for an anti-influenza drug, which may be formulated into a tablet, a capsule, a granule, an oral solution, an injection, a dropping pill, an inhalant, a spray or a drop.

In order to better understand the essence of the present invention, the following pharmacological experiments and results of the patchouli carbon dioxide supercritical extract group, patchouli oil, and baicaleol are described to illustrate the preparation of anti-influenza drugs and anti-avian influenza drugs. The role of. Experimental Example 1 Animal Experiment——Study on the Anti-influenza Virus Effect of Patchouli Carbon Dioxide Supercritical Extract Group, Patchouli Oil and Baiqiu Liol

1 materials and instruments

1. 1 reagent: Baiqiu Li alcohol, purchased from China National Institute for the Control of Pharmaceutical and Biological Products, purity 98%; patchouli carbon dioxide supercritical (SPE-C0 ₂ ) extract (homemade); Baiqiu Li alcohol (homemade), Purity 90%; patchouli oil, commercially available.

1. 2 Instrument: 1-5L supercritical carbon dioxide extraction device, purchased from Guangzhou Light Industry Research Institute; atmospheric separation chromatography column; volatile oil extraction device; US VARIAN-3900 gas chromatograph.

1. 3 strain of influenza A virus mouse lung adaptation strain provided by the Virus Room of Institute of Tropical Medicine, Guangzhou University of Chinese Medicine, After the mice increased their virulence, they were passaged twice in the chicken embryoal allantoic cavity.

1. 4 Animals:: NIH mice, single sex, provided by the Guangdong Medical Laboratory Animal Center, SPF level. Mouse feed is a multi-ingredient formula. Feeding environment: room temperature 23 ± 2 ° C, relative humidity 75 ± 10%.

2 sample preparation

2. 1 Preparation of Baiqiu Liol (Homemade) Sample:

The patchouli is pulverized into coarse powder, passed through a 10-mesh sieve, and taken in 2000g. In a supercritical extraction device, supercritical extraction is carried out with C0 ₂ . The extraction conditions are: extraction pressure 12-30Mpa; extraction temperature 55_60°C _; analytical pressure 5_6Mpa _The analytical temperature was 40-45 ° C. After 2 hours of extraction, the patchouli SPE-C0 ₂ extract containing patchouli alcohol was obtained and placed in a clean container for use.

The extract of patchouli SPE-C0 ₂ was subjected to column chromatography separation: column chromatography was activated by silica gel G at 110 ° C for 1 hour, cooled to room temperature, and then wet-packed with petroleum ether (60-90 ° C). The fraction was eluted with petroleum ether: ethyl acetate: formic acid (20: 1: 0.1). The petroleum ether is recovered under reduced pressure and recrystallized to obtain patchouli alcohol, which is stored at low temperature and protected from light.

Sample GC analysis: American Phenomenex ZB-WAX capillary column (30mX 0. 25mmX 0. 25um), fixed solution polyethylene glycol (Polyethylene Glycol), coating concentration of 100%; temperature programming; initial temperature 150 ° C, keep 23 minutes, ramp up to 230 ° C at 8 ° C per minute for 2 minutes; inlet temperature is 280 ° C, detector temperature is 280 ° C; split ratio is 20 : 1 ; column flow: L OmL Min- 1 ; Carrier gas: N2: 25 mL. min-1 ; Detector: FID, H2: 30mL. min-l, Air: 300mL. min-1 ; Injection volume 2μί, internal standard correction factor method The internal standard is n-octadecane. 5%。 The content of the sample was 94. 65%.

2. 2 Preparation of reagents

Weighed 70mg of Baiqiu Li alcohol (purchased from China National Institute for the Control of Pharmaceutical and Biological Products), and added a proper amount of excipients to prepare a suspension with a concentration of 7%.

Weighed 70mg of Baiqiu Li alcohol (home-made), and added a proper amount of excipients to prepare a suspension with a concentration of 7%.

Weigh 100 mg of patchouli SPE-C0 ₂ extract, and add a suitable amount of excipients to prepare a suspension with a concentration of 10%. Weigh 30mg of patchouli oil and add a proper amount of excipient to prepare a suspension with a concentration of 3%.

3 Methods and results

3. 1 Effect on mouse influenza virus pneumonia

The mice were randomly divided into normal control group, positive drug group (ribavirin), virus control group (ie model group), Baiqiuli alcohol group, Baiqiu Li alcohol (home made) group, patchouli 3?5-(3⁄4 Extract, patchouli oil group, 10 in each group. In addition to the normal control group, each group of mice was lightly anesthetized with ether, and 15 LD50 virus concentrations of the lung-adapted strain of influenza A virus were used to infect mice. 4 drops per dose, about 0. 05 ml. The intragastric administration was started 1 day before infection, and the dose was: 0. lmL/10g, once a day for 4 days, the virus infected control group was steamed in equal volume. Water drenching. Day 5 The body weight of the mice was weighed, the lung lesions were observed by the naked eye, the degree of liver liver consolidation was recorded, the whole lung was weighed, the lung index value was calculated with body weight, and the lung index and inhibition rate were determined. 1.

Lung Index = Mouse Lung Weight (g) / Mouse Body Weight (g) X 100%

Pulmonary index inhibition rate = (control group average lung index - experimental group mean lung index) I control group average lung index X 100% Table 1 Effect of Baiqiu Li alcohol on mouse influenza A virus pneumonia

Number of animals / lung index / ^{x ± s} inhibition rate / % Normal control group 10 0.81 ± 0.14

Virus control group 10 1.52±0· 17##

Ribavirin control group 10 1.09±0.36** 42.69 Patchouli SFE-C0 ₂ extract group 10 1.27±0· 28* 15.90

Patchouli alcohol group 10 1.19±0· 42** 29.23 patchouli alcohol (homemade) group 10 1.21±0· 19** 28.34

Patchouli oil group 10 1.25±0· 47* 20.06

(Compared with the normal control group, ##·· Ρ<0.01; compared with the virus control group:

The mice were sacrificed after 5 days of influenza infection. The lungs of the control group were found to have obvious lung lesions. The lung index was significantly different from the normal control group (P<0.01), indicating that the model was established. Experiments showed that the ribavirin group significantly inhibited lung inflammation, and the lung index was significantly improved compared with the virus control group (P<0.01). The anti-pulmonary inflammatory effects of patchouli and patchouli (home-made) were similar. Compared with the virus control group, the lung index was significantly improved (P<0.01) _{. The} P. sylvestris SPE-C0 ₂ extract group and the patchouli oil group had lower lung index than the virus control group. (Ρ <0· 05).

3.2 Death protection experiment in mice infected with influenza virus

The mice were randomly divided into normal control group, positive drug group (ribavirin), virus control group (ie model group), Baiqiuli alcohol group, Baiqiu Li alcohol (home made) group, patchouli SPE-C0 ₂ extract Group, patchouli oil group, 15 per group. Except the normal control group, each group of mice was lightly anesthetized with ether, and 5 LD50 virus concentrations of the lung-adapted strain of influenza A virus were used, and mice were intranasally inoculated with 4 drops each, about 0.05 ml. The drug was administered by the dose shown in Table 2 from the pre-infection Id, once a day for 12 days, and the virus-infected control group was orally administered with an equal volume of distilled water. The symptoms and deaths of the animals were observed daily for 15 days. Calculate mortality, protection, average survival days, and life extension rates. Death protection rate = (virus infection control group mortality - test group mortality) I virus infection control group mortality life extension rate = (test group average survival days - virus infection control group average survival days I virus infection control group average survival Number of days) X 100%

Table 2 Results of death protection of mice infected with influenza A virus by each sample Give the drug death survival mortality death life-average survival time

(mg * g_l) number (only) number (only) (%) protection rate (%) long rate (%) virus control group 15 0 5.13 ± 2.00 100 - - ribavirin control group 0.07 8 7 10.20 ± 4.69** 53.33 46.67** 98.93 Patchouli SPE-C02

0.1 12 3 7.00±4.42* 80 20 36.45 Extract group

Patchouli alcohol group 0.07 10 5 8.73±4.85** 66.67 33.33** 70.18 Patchouli alcohol (from

0.07 10 5 8.68±4.05** 66.67 33.33** 71.26 system)

Patchouli oil group 0.03 14 1 6.20±3.00* 93.33 6.67 20.86

(Compared with the virus control group, **Ρ<0· 01, *Ρ<0· 05)

After the mice were infected with the influenza virus, most of the symptoms occurred after 4 days. The mice developed symptoms such as shrub, decreased activity, weight loss, and decreased body temperature. From the 6th day onwards, they died. It can be seen from Table 2 that the ribavirin group has obvious death protection and prolongs survival time (P<0.01), and the Baiqiuli alcohol group and Baiqiuli alcohol (homemade) group also have obvious death protection effects. Prolong survival time (P<0.01). The patchouli SPE-C0 ₂ extract group and the patchouli oil group had the effect of prolonging the survival time of life (P<0.05).

3.3 Summary

It can be seen from the above two animal experiments that Baiqiu Liol has obvious therapeutic effect on influenza, and the higher the content of Baiqiuli alcohol, the more obvious the effect, whether it is pure Baiqiu Liol or homemade samples, the effect is Better than the patchouli SPE-C0 ₂ extract group. This also explains from another aspect that the self-made Baiqiu Li alcohol has a higher purity and is equivalent to the antiviral effect of the pure Qiu Li Li alcohol. At the same time, patchouli oil and patchouli 5?5-(3⁄4 extract also have the effect of treating influenza. Experimental example 2 in vitro experiment - inhibition of influenza virus effect in vitro

1. Materials and instruments

1.1 Test: Baiqiu Li alcohol, purchased from China National Institute for the Control of Pharmaceutical and Biological Products, purity 98%; Baiqiu Li alcohol (homemade), purity 90%; patchouli oil, commercially available.

1.2 Instrument: 1-5L supercritical carbon dioxide extraction device, purchased from Guangzhou Light Industry Research Institute; atmospheric separation chromatography column; volatile oil extraction device; US VARIAN-3900 gas chromatograph.

1.3 Strain and cell: Influenza A virus strain is provided by the Virus Laboratory of Tropical Medicine Institute of Guangzhou University of Traditional Chinese Medicine, influenza B virus strain, avian influenza virus (H5N1), cell line MDCK, by Shenzhen Center for Disease Control and Prevention Provided by the Microbiology Laboratory. 2. Sample preparation

2.1 Baiqiu Li alcohol (homemade) Sample preparation:

The extract of patchouli SPE-C0 ₂ was subjected to column chromatography separation: column chromatography was activated with silica gel G at 110 ° C for 1 hour, cooled to room temperature, and then wet-packed with petroleum ether (60-90 ° C). The fractions were collected by elution with petroleum ether: ethyl acetate: formic acid (20: 1: 0.1). The petroleum ether is recovered under reduced pressure and recrystallized to obtain patchouli alcohol, which is stored at low temperature and protected from light.

Sample GC analysis: American Phenomenex ZB-WAX capillary column (30mX0.25mmX0.25um), fixed solution polyethylene glycol (Polyethylene Glycol), coating concentration of 100%; temperature programming; initial temperature 150 ° C, for 23 minutes , ramp up to 230 ° C at 8 ° C per minute for 2 minutes; inlet temperature is 280 ° C, detector temperature is 280 ° C; split ratio is 20:1; column flow: LOmL.min- 1; Carrier gas: N2: 25 mL. min-1; Detector: FID, H2: 30mL. min-l, Air: 300mL. min-1; Injection volume 2μί, internal standard correction factor method to calculate content, internal standard The substance is n-octadecane. As a result, the sample content of Baiqiu Li alcohol (homemade) was 94.65%.

3. Methods and results

3.1 Inhibition of influenza A and B viruses and avian influenza virus in vitro

The SFE-C02 extract of patchouli, the Baiqiu Liol, the Baiqiu Liol (homemade), and the patchouli oil sample were made into a 100 mg/ml sample solution with DMS0, and then diluted with PBS (1:2, 1:8, 1:32, 1:64, 1:128) A total of 4 tubes, after dilution, add 50μ1/well to MDCK cells that have grown into a single layer, then add 50μ1/well of influenza virus solution, plus ΙΟθμΙ / Maintenance solution for the well. Make 4 wells per sample concentration, set the cell control wells, place at 35 °C, 5% C0 ₂ incubator overnight, add 0.2ml% red blood cell suspension to each well, mix well and let stand for 30-60min at room temperature. . Observe the blood coagulation results. The results are shown in Table 3.

The results showed that the two kinds of Baiqiuli alcohol samples had anti-virus effects on influenza A virus, influenza B virus and avian influenza virus. The extract of patchouli SFE-C0 ₂ and patchouli oil also had anti-influenza virus effect. But the effect is weaker than Baiqi Li alcohol. Table 3 Maximum dilution of Baiqiu Liol against influenza virus

Patchouli SFE-C0 ₂ Extract 1: 640 1 : 640 1 : 640

Baiqiu Li alcohol 1: 1280 1: 1280 1: 1280 Baiqiu Li alcohol (homemade) 1: 1280 1: 1280 1: 1280

Patchouli oil 1: 640 1: 640 1: 640

3.2 Determination of cytotoxicity of Baiqiu Li alcohol

Microculture is used. MDCK cells were cultured in 96-well culture plates in advance, cultured in a 5% CO 2 incubator at 35 ° C, and after 24 hours of cell layering, the wells were discarded and diluted (1:20). , 1:40, 1:80, 1:160, 1:320, 1:640, 1:1280) ΙΟθμΙ百秋李 alcohol sample solution, respectively added to the MDCK cells that have grown into a single layer, each hole is added Ιθθμΐ/well maintenance solution. For each concentration of 4 wells, set the cell control wells, set at 35 °C, 5% CO2 incubator environment, record the cytopathic results every day, until the toxicity of the drug to the cells no longer progresses. The minimum dilution factor for drugs that do not have cytopathic effects is an infinite amount of toxicity.

The results showed that no lesions were found in the cells under the respective concentrations, indicating that cytotoxicity was not cytotoxic.

3.3 Summary

In vitro experiments have shown that Baiqiu Li alcohol has a toxic effect on influenza A virus, influenza B virus and avian influenza virus, and is not cytotoxic.

It can be inferred from the above experiments that the main component of the extract of patchouli, patchouli oil, and patchouli SPF-C0 ₂ which exerts anti-cold virus action is patchouli alcohol, namely, baqiol.

The advantages of the invention are as follows: (1) The present invention finds that the patchouli carbon dioxide supercritical extract group, patchouli oil or baiqiuol has significant inhibition and killing of influenza A virus, avian influenza virus and influenza B virus. The role of the virus has opened up a new field of application. (2) The patchouli carbon dioxide supercritical extract group, patchouli oil or baicale alcohol of the invention is safe and non-toxic, and has strong pharmacological action, indicating a good medicinal prospect. (3) The raw material of the invention is rich in source, low in price, and simple in preparation process. (4) The pharmaceutical composition of the present invention can be prepared into an oral dosage form, an injection dosage form, a tablet U, a capsule, etc., and is convenient to use. detailed description

Example 1

The patchouli is pulverized into coarse powder, passed through a 10-mesh sieve, taken at 2000g, placed in a supercritical extraction device, and supercritically extracted with C02. The extraction conditions are: extraction pressure 12-30Mpa; extraction temperature 55_60°C _; analytical pressure 5_6Mpa; Analytical temperature After extracting for 4 hours at 40-45 ° C, a patch of patchouli SFE-C02 containing patchouli alcohol was obtained and placed in a clean container for use. Take the above-prepared extract of patchouli SFE-C0 ₂ 2000mg, add appropriate excipients, dissolve in 1000ml of water to make an aqueous solution, dissolve, mix well, and then dispense into a pellet of 2mg/lml / concentration, seal , disinfection. During the flu epidemic, it is used to drip into the nasal cavity to prevent and treat the flu. Example 2

The extract of patchouli SFE-C0 ₂ prepared in Example 1 was added to a suitable excipient, dissolved in 1000 ml of water to prepare a 0.2% aqueous solution, dissolved, and uniformly mixed, sealed and sterilized. Used during the flu epidemic to inject, prevent or treat the flu. Example 3

200 mg of patchouli SFE-C0 ₂ extract prepared in Example 1 was added, and appropriate excipients were added to prepare a soft capsule containing 5% of patchouli alcohol, sealed and sterilized. Used during oral flu, prevention or treatment of influenza. Example 4

200 mg of the patchouli SFE-C0 ₂ extract prepared in Example 1 was tableted, tableted, sealed, and sterilized. Used during oral flu, prevention or treatment of influenza. Example 5

The extract of patchouli SFE-C02 was prepared in Example 1 and subjected to column chromatography. Column chromatography was carried out by using silica gel G at 110 ° C for 1 hour, and after cooling to room temperature, petroleum ether (60-90 ° C) was used. It was wet-packed and eluted with petroleum ether: ethyl acetate: formic acid (20: 1: 0.1) to collect fractions. The petroleum ether is recovered under reduced pressure and recrystallized to obtain patchouli alcohol, which is stored at low temperature and protected from light.

Take 1500mg of Baiqi Lialol prepared above, add appropriate auxiliary materials, dissolve in 1000ml water to make an aqueous solution, dissolve, mix well, and then pack into 3ppm/2ml/dose dropping pills, seal and disinfect. During the flu epidemic, it is used to drip into the nasal cavity to prevent and treat the flu. Example 6

Take 150 mg of baicaleol prepared in Example 2, add suitable excipients, dissolve in 1000 ml of water to make a 0.1% aqueous solution, dissolve, mix well, seal and disinfect. During the flu epidemic, it is used for injection, prevention and treatment of influenza. Example 7

Take 150 mg of Baiqi Li alcohol prepared in Example 2, add suitable excipients, and prepare soft capsules containing 5% of patchouli alcohol, sealed and disinfected. Used during oral flu, prevention or treatment of influenza. Example 8

Take 150 mg of baicaleol prepared in Example 2, add suitable excipients, compress tablets, make tablets, seal and disinfect. Used during oral flu, prevention or treatment of influenza. Example 9

Buy commercially available patchouli oil lOOOOmg, add appropriate excipients, dissolve in 1000ml water to make an aqueous solution, dissolve, mix evenly, and then dispense into lmg/lml/dose dropping pills, seal and disinfect. During the flu epidemic, it is used to drip into the nasal cavity to prevent and treat the flu.

Example 10

Buy commercially available patchouli oil 100mg, add appropriate excipients, dissolve in 1000ml water to make 0.1% aqueous solution, dissolve, mix evenly, seal and disinfect. During the flu epidemic, it is used for injection, preventive treatment. Example 11

Purchase 100mg of commercial patchouli oil, add appropriate excipients, and prepare soft capsules containing 5% of patchouli alcohol to seal and disinfect. Used during oral flu, prevention or treatment of influenza. Example 12

Buy commercially available patchouli oil, add appropriate excipients, compress tablets, and make tablets for oral prevention and treatment of influenza. Example 13

The extract of patchouli SFE-C02 prepared in Example 1 was mixed with the chicken feed, and the daily dose was 0.16 g / of the extract of patchouli SFE-C02, which was taken twice in the morning and evening. Day, used to prevent bird flu. Example 14

Take the puppies prepared in Example 5, mix them well with the chicken feed, and use the daily dose of Baiqiu Li alcohol 0. lg / only, take it twice in the morning and evening, and take it for 3 days in a row to prevent bird flu. Example 15

Buy the commercial patchouli oil, mix it with the pig's feed, use the patchouli sesame oil 0. 5g / day, take it twice in the morning and evening, and take it for 3 days in a row to prevent bird flu. The above-described embodiments are merely preferred embodiments of the present invention, and are not intended to limit the scope of the present invention, and equivalent changes or modifications made by the features and principles of the present invention should be included in the present invention. Within the scope of the claims.

Claims

Rights request

1. The application of Baiqiu Li alcohol in the preparation of a medicament for treating or preventing influenza.

2. The application of patchouli carbon dioxide supercritical extract in the preparation of drugs for the treatment or prevention of influenza.

3. The application of patchouli oil in the preparation of drugs for treating or preventing influenza.

4. Use of a composition comprising the active active ingredient, baicaleol, and a pharmaceutically acceptable carrier for the preparation of a medicament for the treatment or prevention of influenza.

5. The application of Baiqiu Li alcohol in the preparation of influenza drugs caused by treating or preventing influenza A virus.

6. The application of Baiqiu Li alcohol in the preparation of influenza drugs caused by treating or preventing influenza B virus.

7. The use of Baiqiu Li alcohol in the preparation of influenza drugs caused by the treatment or prevention of avian influenza virus.

A composition for an anti-influenza drug, characterized in that the composition comprises the active active ingredient, baicalol, and/or a pharmaceutically acceptable carrier.

The composition for anti-influenza drugs according to claim 8, wherein the active ingredient octoberol raw material is derived from a patchouli carbon dioxide supercritical extract, a broad eucalyptus oil or a broad fragrant incense.

The anti-influenza drug composition according to claim 8, wherein the composition of the drug is formulated into tablets, capsules, granules, oral liquids, injections, dropping pills, inhalants, Spray or drop.