WO2010086190A1

WO2010086190A1 - Method and device for detecting a substance applied onto a body part

Info

Publication number: WO2010086190A1
Application number: PCT/EP2010/000693
Authority: WO
Inventors: Thomas Zimmermann
Original assignee: Thomas Zimmermann
Priority date: 2009-01-30
Filing date: 2010-01-29
Publication date: 2010-08-05

Abstract

A method and a device for detecting a substance applied to a body part are provided. The method comprises taking images of the body part and detecting the substance applied thereto by recognizing spectral-specific properties of the substance in the images taken. The taking the images of the body part is conducted in the ultraviolet spectral range, in the visible spectral range and in the infrared spectral range. In order to detect the spectral-specific properties of the substance, the entire detected spectral range is used. The device for detecting at least one substance applied to body parts comprises: an ultraviolet luminaire (1), an imaging device (2, 3, 4) for taking images of the body part in the ultraviolet spectral range, in the visible spectral range and in the infrared spectral range, and at least one analysis unit (6), which is connected to the imaging device (2, 3, 4), for receiving the images taken, so as to analyze the images with regard to spectral-specific properties of substances potentially applied to said body part.

Description

Method and device for detecting a substance applied to a body part

The present invention relates to a method and a device for detecting a substance applied to a body part, in particular a method and a device for detecting applied substances for the detection of applied doping-relevant substances in sports.

Doping agents are available in various forms of administration. Above all, the administration by syringes is generally known. In addition, there are other forms of administration for doping agents, such as the rubbing of body regions with ointments, for example. To increase or decrease their sensitivity to pain as needed. For example, in equestrian sports at show jumping competitions, the application of capsaicin in the form of ointments or creams in the area above the hooves on the front legs has become known. In the treated areas, the sensitivity of the horse increases, so that this has an additional motivation not to touch a pole.

It is therefore an object of the present invention to provide a method and a device with which substances applied to body parts can be detected reliably and rapidly.

This object is achieved by a method for detecting one on one

Body part applied substance according to claim 1 or a

Device for detecting a substance applied to a body part according to claim 9. The dependent claims contain advantageous embodiments of the invention.

In the method according to the invention for detecting a substance applied to a body part, taking pictures of the body part and detecting the applied substance takes place on the basis of a recognition of spectrally specific properties of the substance in the recorded images. The images of the body part are recorded in the ultraviolet (UV) spectral range, in the visible (VIS) spectral range and in the infrared (IR) spectral range. To recognize the spectral specific properties, the entire spectral range in which the images are taken is used. The spectral region is preferably a contiguous spectral region extending beyond the visible spectral region into the ultraviolet and infrared spectral regions. In this case, the entire contiguous spectral range should extend at least from 280 nm to 1400 nm, preferably at least from 100 nm to 1400 nm and in particular at least from 100 nm to 3000 nm.

The method according to the invention can be considered as a working method of optical spectroscopy, which is based on the characteristic absorption of radiation in the visible and invisible infrared and ultraviolet spectral range by inorganic and organic compounds. In the process, characteristic electron transitions and UV reflections are detected for the corresponding substances. If a substance absorbs light in the visible range, it is colored. If a substance reflects light in the UV range, this is the proof of the existence of a substance. If the spectral property and the wavelength are determined (measured) by means of a suitable UV camera, the substance can be identified by comparison with known spectral properties of certain substances.

The new method allows the short-term / immediate detection and identification of "non-body" substances (doping-relevant substances) against endogenous substances such as blood, lymph, tears, water and sweat on the skin or on / in the coat or hair. The conventional doping sample following the spectrophotometric, optical test (blood, urine) is indicated with this method if positive detection of a doping-relevant substance is carried out.

The method according to the invention is based on the following finding:

The spectrum of a substance in the UV / VIS spectral range is often characteristic. For example, e.g. For organic molecules, the presence of UV absorption indicates the presence of a π-electron system and the strength and location of the absorption is a measure of its extent. With a known basic molecule, substituents can be recognized by their influence on the spectrum. The prominence of reflection can also provide information about the intensity.

However, a UV / VIS spectrum is rarely interpretatively analyzable alone and thus can not be the sole basis of a substance identification without complementary suitable methods such as IR, MS or NMR spectra. However, the UV / VIS spectrum, which can be measured easily and with little substance, alone can be a good confirmation of another substance identification. (Quick detection of the detection and prominence of a non-endogenous, applied substance.)

The relatively limited sole significance of a UV / VIS spectrum is primarily due to the fact that the UV / VIS spectrum only a part of the

Mapping molecules of a substance. In addition, UV / VI S spectra can be very strongly solvent-dependent and can also be falsified by other interference factors, for example electromagnetic interference. Thus, UV / VI S spectra usually provide only part of the total spectral range of evaluable information about a particular substance, with only a few

Absorbance and reflection bands obtained, imaged

Readings.

Taking pictures in the ultraviolet spectral range, in the visible

Spectral range and in the infrared spectral range can be done using different recording devices. Using different recording devices allows each Adapting recording device specifically to the respectively recorded spectral range, so that a high sensitivity can be achieved in each spectral range.

It is advantageous when taking the pictures in the ultraviolet spectral range, in the visible spectral range and in the infrared

Spectral range takes place simultaneously with the same recording rate, so that, for example, for each recording in the visible spectral range a simultaneous

Recording in the remaining spectral ranges is present. The simultaneous

Recording can be achieved by suitable triggering of the respective recordings.

In order to generate a judicious video evidence, the recorded images and / or the detected spectral-specific properties and / or the substance detected on the basis of the detected spectral-specific properties can be stored along an identical time code for all.

The inventive method is basically suitable for veterinary and human applications.

A device according to the invention for detecting at least one substance applied to parts of the body comprises a UV light, a recording device for taking pictures of the body part in the ultraviolet spectral range, in the visible spectral range and in the infrared spectral range and at least one analysis device connected to the receiving device for receiving the recorded images Analyze the images for spectral specific properties of substances potentially applied to the body.

The device according to the invention makes it possible to carry out the method according to the invention and therefore permits the realization of the advantages and characteristics of the invention described with reference to the method.

The recording device can be a UV camera, a visual camera and an infrared camera, which allows to use the most suitable sensor for each of the three spectral ranges.

Preferably, a trigger device is provided, which is connected for the timed triggering of recordings in the ultraviolet spectral range, in the visible spectral range and in the infrared spectral range with the UV camera, the visual camera and the infrared camera. As a result, simultaneous recordings can be realized with the same recording rate in all three spectral ranges.

The optics of the UV camera, the visual camera and the infrared camera can also be arranged geometrically synchronized.

The spectral recording area of the recording device should extend at least from 280 nm to 1400 nm, preferably at least 100 nm to 1400 nm and in particular at least 100 nm to 3000 nm, in order to reliably detect spectral-specific properties of substances applied to the body part.

To counteract a deterioration of the evidential force of the device, the receiving device and the analysis device can be combined in a housing, shielded and secured against manipulation.

The device according to the invention may also comprise at least one mirror, which is arranged in relation to the receiving device in such a way that a region remote from the receiving device can also be received.

Further features, properties and advantages of the present invention will become apparent from the following description of an embodiment with reference to the accompanying figure.

FIG. 1 shows a device according to the invention for detecting at least one substance applied to body parts in a schematic representation.

Hereinafter, an embodiment of a device for Detecting at least one applied to body parts substance explained with reference to Figure 1. The device shown in the figure comprises a UV lamp 1 and a recording device for recording images, which consists in the present embodiment of a UV camera 2, a video camera 3, an IR camera 4 and a combined switch and trigger unit 5. The cameras 2, 3, 4 preferably have digital image sensors, so that the recorded images are in the form of digital electrical signals. In addition, the device comprises a computer 6 connected to the recording device for receiving the digital signals representing the images. This computer is equipped with software for analyzing the images obtained with the recording device with regard to spectral specific properties of substances potentially applied to the body part The computer 6 is also connected to a screen 7, on which the analysis results can possibly be displayed together with other relevant information. In the present embodiment, the screen is also designed as a touch screen to make entries in the computer 6 can. But it is also possible to provide a standalone keyboard, rather than form the screen as a touch screen. In addition, the device comprises three mirrors 8, 9, 10, which are arranged at a distance from the receiving device and serve to make such areas, which can not be viewed directly by the cameras, also accessible to the recording. Each of the three mirrors 8, 9, 10 is assigned to one of the three cameras 2, 3, 4.

The illustrated in Figure 1 embodiment of the device according to the invention is adapted for use in equestrian sport. Between the cameras 2, 3, 4 and the mirrors 8, 9, 10, an alley 11 is arranged, through which the horses are led during doping test. The cameras and the mirrors are arranged at the level of the horse's legs, so that the cameras 2, 3, 4 can, for example, image the leg sections over the hooves in order to be able to detect any applied doping substances. With the help of the Speiegel 8, 9, 10 can be imaged both sides of the legs. An exemplary embodiment of the method according to the invention will be described below with reference to the system function of the device shown in FIG.

The UV camera 2, the visual camera 3 and the infrared camera 4 take at the same time with an adjusted frame rate on the target in focus, for example, the legs of a horse on. During the recording / measurement, the UV lamp 1 is directed to the measurement object, or switched. The output signals of the cameras (data) are combined in the switch / trigger port 5 and transmitted via a data line to the computer 6. The computer 6 takes over the further processing of the signals as an evaluation unit by means of the installed evaluation software for each camera. About the computer 6, the cameras 2, 3, 4 are controlled and programmed. The screen 7 displays online in real time all recorded camera images as well as a histogram of the current UV camera data. The evaluation unit thus also functions as a control and operating unit. The present in Figure 1 mirror 8, 9, 10 are not mandatory, but only in case of need to supplement and expand the focus to order.

In a special automated embodiment, the method is characterized in that

a) each individual imaging procedure of its spectral property and sensitivity is displayed and read out accordingly on a screen,

b) the entire spectral range from <100nm to 14μ (1400nm) and possibly even to 30μ (3000nm) is completely detected and displayed,

c) a software permits the storage of the transmitted data of all system units along a time code which is the same for all system units,

d) an evaluation software that allows immediate analog or automatic detection of certain spectra or spectral specific properties of applied substances in the imaging process, eg. based on a comparison of spectrally specific properties detected with stored in the memory of the computer spectral specific properties of different doping substances,

e) all cameras, sensors and detectors are grouped in a housing, shielded and secured against tampering, and

f) the optics of the cameras are mounted geometrically synchronized.

there

g) detects the integrated into the system infrared camera (thermal camera) the spectral range according to liquids (including creams,

Gels, etc), which cause a change in the density of the skin by their mode of action (active substances), whereby the thermal structure change of the surface in the infrared image is made visible;

h) detects the integrated into the system UV camera (UV / VIS) in the spectral range according to liquids (including creams, gels, etc), by their mode of action and chemical composition (active and

Excipients) have specimen-specific spectral reflective properties (cause reflections) which can be visualized, identified and assigned using a suitable UV illuminant (commercially available);

i) the integrated into the system visual camera (video camera) acts as an integrated control method, the

Ensures the judiciary video evidence manifested in the time code,

- allows a safe detection of the measured object (animal / human),

Color depths, surface deviations, inhomogeneities and differences in the surface structure (due to injuries, scars, missing fur, hair etc), powder applications, and others Buildup and foreign bodies that can affect the measurements,

can be consulted for error detection and proof,

- Captures environmental conditions and any interferences relevant to the optics, sensors and detectors in the image (RGB / VGA, video);

j) are optionally by using one or more suitable mirrors in the arrangement of the measurement setup (test or detection run) and the camera optics opposite sides and areas of

Test object made visible.

Individual features of this combination may also be omitted or replaced by other suitable features.

As already mentioned, the method should be used primarily in equestrian sport. Here conventional doping samples (blood, urine) are taken.

Since 01/2009 there is the rule of the FEI (Federation Equestre

International) also infrared tests of all competing in the competition / tournament horse perform in order to detect any applied substances. This requirement has international validity for all professional and Olympic equestrian competitions and tournaments. As described, however, the sole examination by means of an infrared camera covers only a part of the relevant spectral range. The extension of the test area thus provides greater safety in the detection of applied substances and thus also serves to comply with international animal protection laws. Already the publication on the existence of suitable test procedures could be considered in the past as a preventive measure.

The IR / UV / VIS spectrophotometry as a doping test in equestrian sports or supplementary procedure is likely to prevail because of the variety of new, including therapeutic and diagnostic detection variants. By means of the exemplary embodiment explained with reference to the exemplary embodiments, the simultaneous coupling of three individual imaging methods into a system structure, the recording, the image superimposition, the control functions and the evaluation. The coupling of a UVΛ / IS camera with an infrared camera and a visual camera under the same triggering offers for the first time the complete detection and identification of applied substances by non-invasive imaging techniques, with immediate evaluation results. The obtained data and image results from this new process are completely reproducible. The system can detect both analog and automatic.

In analog detection, individual units can also work decentrally (manually). The manual operation of the thermal camera is practice-oriented with coupled diagnostic support and can optimize the result of the IR imaging procedure.

The manual operation of the visual (video) camera as a control method ensures the judicious video evidence and enables a reliable detection of the measured object (animal / human), of color depths and surface deviations. In addition, the course and the performance of the measurement are documented.

The manual operation of individual system components depends on room, position, lighting conditions and other external disturbances such as wind, rain (moisture) at the time of measurement on site and serves to optimize the measurement.

As already mentioned above, the recognition of specific spectra or spectrally specific properties of applied substances in the imaging process, for example, based on a comparison of detected spectrally specific properties can be made possible with stored in the memory of the computer spectral specific properties of various doping substances. With regard to the spectral-specific properties of different doping substances, it is possible in particular to make use of extensive literature with spectral libraries. Examples of such spectral libraries, in particular for UV / VIS spectrometry are mentioned at the end of the description. UVΛ / IS spectrometry has a wealth of experience as it has long been the only practical method of instrumental analysis. Especially in the fifties, many scientific papers dealt with the qualitative aspects of UV / VIS spectrometry, resulting in a large number of printed spectra collections. In part, these are only collections of literature, in part tables of absorption maxima with the respective ε values, together with other data as well. Particularly noteworthy is the large Sadtler Spectrum Catalog with over 100,000 mapped spectra. However, there are also good overviews of the compact Sadtler catalog and the atlas of Perkampus. Likewise, collections are available for limited applications, such as the spectrum catalog of pharmaceutical active ingredients and excipients from Dibbern or the data collections for the UV / VI S spectra of proteins or natural products. Digital UVΛ / IS spectra collections are rarely kept; accordingly, there are only a few approaches to develop and provide software for spectral library search.

The listed data collections for qualitative UV / VIS spectroscopy provide an overview of those in the German-speaking world particularly in

Certain monographs and other language areas contain further, extensive collections of data; many example spectra are also included in the textbooks of UV / VIS spectrometry. But even in the original literature, the UV / VIS spectrum is usually one of the

Documentation of a new connection. Spectral libraries:

P. Grammaticakis

Specters ^d'absorption Ultra Violet de Compose Organiques Azotes Correlations Spectrochimiques, Part 1-3 Technique et Documentation, Paris, 1979

Listed are 4000 different spectra of organic nitrogen compounds

Sadtler Standard Ultraviolet Spectra

Sadtler Research Laboratories Inc., Philadelphia 1972

William W. Simons

The Sadtler Handbook of Ultraviolet Spectra Sadtler Research Laboratory Inc., Philadelphia

API Research Project 44 Ultraviolet and Visible Data Carnegu Institute and U.S. Pat. National Bureau of Standards

W. Neudert, H. Röpke Steroid Spectra Atlas Springer Verlag, Berlin 1965 IR spectra and UV peak data of 1111 compounds.

Hans-Werner Dibbern UV and IR spectra of important pharmaceutical agents Editio Cantor, Aulendorf 1978

I. Sandemann, H.H. Perkampus DMS UV Atlas of Organic Compounds, Vol. I-V Verlag Chemie, Weinheim; Butterworth, London, 1966 Compilation of 1000 representative UV spectra

J.G. Graselli, W.M. Ritchey

Atlas of Spectral Data and Physical Constants of Organic Compounds CRC Press, Cleveland, Ohio, USA, (2nd ed), Vol. I Listed are the UV / VIS maxima, the solvent and the Sadtler reference no. W. Karcher, RJ. Fordham, et al

Spectral Atlas of Polycyclic Aromatic Compounds

D.Reidel Publishing Company, Dordrecht / Boston / Lancaster

R.A. Friedel, M. Orchin Ultraviolet Spectra of Aromatic Compounds Wiley + Sons, 1951

H.M. Hershenson

Ultraviolet and Visible Absorption Spectra Academic Press New York 1956, etc. Index of (1930-1954) 1961, Index of (1955-1959) 1962, Index of (1959-1961)

L. Lang

Absorption Spectra in the Ultraviolet and Visible Region Adademiai Kiado, Budapest 1958-84 26 volumes of 200 Ig D spectra, each with registration conditions

J.Pliva, M. Horak, V. Herout, F. Sorm

The terpenes, collection of spectra and physical constants part l + ll

Akademie Verlag Berlin, 1960 The IR spectra and extinction coefficients of the UV maxima are listed

International Union of Pure and Applied Chemistry (IUPA C)

Tables of Spectrophotmetric Absorption Data of the Compounds used for the

Colorimetric Determination of Elements

Butterworth, London 1963

J.Holubek, O.Stroufs

Spectral Data and Physical Constants of Alkaloids Heyden + Sons, London 1965 HE disgrace, MJ. Kamlet Organic Electronic Spectral Data Interscience Publishers, New York 1946-1984 (cont.) Bibliography of 400,000 spectra representing approximately 95% of all published spectra

K. Hirayama

Handbook of Ultraviolet and Visible Adsorption Spectra of Organic Compounds

Plenary Press Data Division, New York 1967 Listed are 13,000 typical chromophore compounds

K. Yamaguchi

Spectral Data of Natural Products

Elsevier Publ. Comp. Amsterdam, 1970, VoI I

UV, IR and other reference spectra of natural products

D.M.Krschbaum

Molar Absorptivity and A (1/1) Values for Proteins at Selected Wavelengths of the Ultraviolet and Visible Regions XVI

Anal. Biochem. 90 (1978), 309-330

Characteristic wavelengths of more than 150 proteins, citation of 15 previous publications

German Research Foundation Dyes for Food, 2nd edition Verlag Chemie, Weinheim 1988 IR + UV spectra of food dyes

H.H.Perkampus

UV / VIS Adas of Organic Compounds, Volume 1+ 2 Verlag Chemie, Weinheim (2nd edition 1992)

LW. Robinson

Handbook of Spectroscopy CRC Press, Cleveland, Ohio, USA

Claims

claims

A method for detecting a substance applied to a body part, which comprises taking pictures of the body part and detecting the applied substance on the basis of a recognition of spectrally specific properties of the substance in the recorded images, wherein the taking of the images of the body part in the ultraviolet spectral range, in visible spectral range and in the infrared spectral range and is used to detect the spectral specific properties of the entire detected spectral range.

2. The method of claim 1, wherein the taking of the images in a continuous spectral range, which extends beyond the visible spectral range in the ultraviolet and the infrared spectral range.

3. The method of claim 2, wherein the entire contiguous spectral range extends at least from 280nm to 1400nm.

4. The method of claim 3, wherein the entire contiguous spectral range extends at least from 100nm to 1400nm.

5. The method of claim 3 or claim 4, wherein the entire contiguous spectral range extends at least from 100nm to 3000nm.

6. Method according to one of the preceding claims, in which the images are recorded in the ultraviolet spectral range, in the visible spectral range and in the infrared spectral range using different recording devices (2, 3, 4).

7. The method of claim 6, wherein the taking of the images in the ultraviolet spectral range, in the visible spectral range and in the infrared spectral range in each case takes place simultaneously with the same recording rate.

8. The method according to any one of the preceding claims, in which a storage of the recorded images and / or the detected spectral specific properties and / or the substance detected on the basis of the detected spectral specific properties substance along a same time code takes place.

9. A device for detecting at least one applied to body parts substance comprising: a UV lamp (1), - a recording device (2, 3, 4) for recording images of the body part in the ultraviolet spectral range, in the visible and in the infrared spectral range, and an analysis device (6) connected to the recording device (2, 3, 4) for receiving the recorded images for analyzing the images in terms of spectral-specific

Properties of substances potentially applied to the body.

10. Apparatus according to claim 9, in which the receiving device comprises a UV camera (2), a visual camera (3) and an infrared camera (4).

11. The device according to claim 10, in which a trigger device (5) is provided, which for the timed triggering of recordings in the ultraviolet spectral range, in the visible spectral range and in the infrared spectral range with the UV camera (2), the visual camera (3). and the infrared camera (4) is connected.

12. The device according to claim 10 or claim 11, in which the optics of UV camera (2), the visual camera (3) and the infrared camera (4) are geometrically synchronized.

13. Device according to one of claims 9 to 12, in which extends the spectral recording area of the receiving device (2, 3, 4), at least from 280nm to 1400nm.

14. Device according to one of claims 9 to 13, in which the receiving device (2, 3, 4) and the analysis device (6) are combined in a housing, shielded and secured against manipulation.

15. Device according to one of claims 9 to 15, in which in addition at least one mirror (8, 9, 10) is provided, which is arranged with respect to the receiving device (2, 3, 4) that also one of

Receiving device (2, 3, 4) facing away from the body parts can be added.