WO2010065470A3 - Compositions and methods for detecting background male dna during fetal sex determination - Google Patents

Compositions and methods for detecting background male dna during fetal sex determination Download PDF

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Publication number
WO2010065470A3
WO2010065470A3 PCT/US2009/066137 US2009066137W WO2010065470A3 WO 2010065470 A3 WO2010065470 A3 WO 2010065470A3 US 2009066137 W US2009066137 W US 2009066137W WO 2010065470 A3 WO2010065470 A3 WO 2010065470A3
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WO
WIPO (PCT)
Prior art keywords
compositions
methods
male dna
fetal
dna during
Prior art date
Application number
PCT/US2009/066137
Other languages
French (fr)
Other versions
WO2010065470A2 (en
Inventor
Christina Mork
Chinh Bach
Preston Al Baecker
Anna Vitebsky
Meng Hou
Original Assignee
Consumer Genetics, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consumer Genetics, Inc. filed Critical Consumer Genetics, Inc.
Publication of WO2010065470A2 publication Critical patent/WO2010065470A2/en
Publication of WO2010065470A3 publication Critical patent/WO2010065470A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

This invention provides compositions and methods for distinguishing fetal from non-fetal male DNA in a human maternal whole blood sample. The invention provides isolated oligonucleotides and compositions thereof complementary to a centromeric alphoid repeat sequence of the human Y chromosome, which can be used to hybridize, amplify and/or detect non-fetal male DNA present in a blood sample in an amplification assay. The invention also provides methods for detecting or determining the presence or absence of non-fetal DNA in a maternal blood sample.
PCT/US2009/066137 2008-12-01 2009-11-30 Compositions and methods for detecting background male dna during fetal sex determination WO2010065470A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11900108P 2008-12-01 2008-12-01
US61/119,001 2008-12-01

Publications (2)

Publication Number Publication Date
WO2010065470A2 WO2010065470A2 (en) 2010-06-10
WO2010065470A3 true WO2010065470A3 (en) 2010-12-29

Family

ID=42233803

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/066137 WO2010065470A2 (en) 2008-12-01 2009-11-30 Compositions and methods for detecting background male dna during fetal sex determination

Country Status (1)

Country Link
WO (1) WO2010065470A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
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US8962247B2 (en) 2008-09-16 2015-02-24 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non invasive prenatal diagnoses

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US8476013B2 (en) 2008-09-16 2013-07-02 Sequenom, Inc. Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
WO2011087760A2 (en) 2009-12-22 2011-07-21 Sequenom, Inc. Processes and kits for identifying aneuploidy
WO2012177792A2 (en) 2011-06-24 2012-12-27 Sequenom, Inc. Methods and processes for non-invasive assessment of a genetic variation
WO2013052663A1 (en) * 2011-10-04 2013-04-11 Qiagen Gmbh Methods and compositions for detecting a target dna in a mixed nucleic acid sample
WO2013052907A2 (en) 2011-10-06 2013-04-11 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9984198B2 (en) 2011-10-06 2018-05-29 Sequenom, Inc. Reducing sequence read count error in assessment of complex genetic variations
US10196681B2 (en) 2011-10-06 2019-02-05 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9367663B2 (en) 2011-10-06 2016-06-14 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US10424394B2 (en) 2011-10-06 2019-09-24 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US8688388B2 (en) 2011-10-11 2014-04-01 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
EP2766496B1 (en) * 2011-10-11 2017-03-08 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
ES2929923T3 (en) 2012-01-20 2022-12-02 Sequenom Inc Diagnostic processes that condition the experimental conditions
EP4155401A1 (en) 2012-03-02 2023-03-29 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
EP4276194A3 (en) * 2012-05-21 2024-03-06 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US10504613B2 (en) 2012-12-20 2019-12-10 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9920361B2 (en) 2012-05-21 2018-03-20 Sequenom, Inc. Methods and compositions for analyzing nucleic acid
US10497461B2 (en) * 2012-06-22 2019-12-03 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
CA2878979C (en) 2012-07-13 2021-09-14 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
US10482994B2 (en) 2012-10-04 2019-11-19 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US20130309666A1 (en) 2013-01-25 2013-11-21 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
WO2014168711A1 (en) 2013-03-13 2014-10-16 Sequenom, Inc. Primers for dna methylation analysis
ES2939547T3 (en) 2013-04-03 2023-04-24 Sequenom Inc Methods and procedures for the non-invasive evaluation of genetic variations
WO2014190286A2 (en) 2013-05-24 2014-11-27 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
ES2721051T3 (en) 2013-06-21 2019-07-26 Sequenom Inc Method for the non-invasive evaluation of genetic variations
BR112016007401B1 (en) 2013-10-04 2023-04-11 Sequenom, Inc. METHOD FOR DETERMINING THE PRESENCE OR ABSENCE OF A CHROMOSOMAL ANEUPLOIDY IN A SAMPLE
US10438691B2 (en) 2013-10-07 2019-10-08 Sequenom, Inc. Non-invasive assessment of chromosome alterations using change in subsequence mappability
EP3117011B1 (en) 2014-03-13 2020-05-06 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US11783911B2 (en) 2014-07-30 2023-10-10 Sequenom, Inc Methods and processes for non-invasive assessment of genetic variations
WO2018022890A1 (en) 2016-07-27 2018-02-01 Sequenom, Inc. Genetic copy number alteration classifications
CA3207879A1 (en) 2017-01-24 2018-08-02 Sequenom, Inc. Methods and processes for assessment of genetic variations
US20200299677A1 (en) * 2017-10-27 2020-09-24 Juno Diagnostics, Inc. Devices, systems and methods for ultra-low volume liquid biopsy

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WO2002067665A2 (en) * 2001-02-23 2002-09-06 Infigen, Inc. Cloning of transgenic animals comprising artificial chromosomes

Patent Citations (1)

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WO2002067665A2 (en) * 2001-02-23 2002-09-06 Infigen, Inc. Cloning of transgenic animals comprising artificial chromosomes

Non-Patent Citations (4)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8962247B2 (en) 2008-09-16 2015-02-24 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non invasive prenatal diagnoses

Also Published As

Publication number Publication date
WO2010065470A2 (en) 2010-06-10

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