WO2010059558A2 - Gènes et protéines de défense de plante et procédés d'utilisation - Google Patents

Gènes et protéines de défense de plante et procédés d'utilisation Download PDF

Info

Publication number
WO2010059558A2
WO2010059558A2 PCT/US2009/064554 US2009064554W WO2010059558A2 WO 2010059558 A2 WO2010059558 A2 WO 2010059558A2 US 2009064554 W US2009064554 W US 2009064554W WO 2010059558 A2 WO2010059558 A2 WO 2010059558A2
Authority
WO
WIPO (PCT)
Prior art keywords
plant
sequences
promoter
nucleotide sequence
expression
Prior art date
Application number
PCT/US2009/064554
Other languages
English (en)
Other versions
WO2010059558A3 (fr
Inventor
James English
Carl R. Simmons
Nasser Yalpani
Original Assignee
Pioneer Hi-Bred International, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi-Bred International, Inc. filed Critical Pioneer Hi-Bred International, Inc.
Priority to CA2743770A priority Critical patent/CA2743770A1/fr
Publication of WO2010059558A2 publication Critical patent/WO2010059558A2/fr
Publication of WO2010059558A3 publication Critical patent/WO2010059558A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8239—Externally regulated expression systems pathogen inducible
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8285—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for nematode resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Insects & Arthropods (AREA)
  • Pest Control & Pesticides (AREA)
  • Communicable Diseases (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La présente invention concerne des procédés et des compositions pour moduler le développement et des réponses de défense. La présente invention concerne des séquences nucléotidiques codant pour des protéines de défense de plante. Les séquences peuvent être utilisées dans des cassettes d’expression pour moduler le développement, des voies de développement, et des réponses de défense. La présente invention concerne en outre des plantes transformées, des cellules de plante, des tissus et des semences.
PCT/US2009/064554 2008-11-21 2009-11-16 Gènes et protéines de défense de plante et procédés d'utilisation WO2010059558A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA2743770A CA2743770A1 (fr) 2008-11-21 2009-11-16 Genes et proteines de defense de plante et procedes d'utilisation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11665008P 2008-11-21 2008-11-21
US61/116,650 2008-11-21

Publications (2)

Publication Number Publication Date
WO2010059558A2 true WO2010059558A2 (fr) 2010-05-27
WO2010059558A3 WO2010059558A3 (fr) 2010-10-21

Family

ID=42197634

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/064554 WO2010059558A2 (fr) 2008-11-21 2009-11-16 Gènes et protéines de défense de plante et procédés d'utilisation

Country Status (3)

Country Link
US (3) US20100132070A1 (fr)
CA (1) CA2743770A1 (fr)
WO (1) WO2010059558A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012027209A3 (fr) * 2010-08-23 2012-07-05 Pioneer Hi-Bred International, Inc. Variants inédits de la défensine et leurs procédés d'utilisation

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3379022A1 (fr) * 2017-03-21 2018-09-26 Welltec A/S Système de capteur de fond de trou
EP3379025A1 (fr) 2017-03-21 2018-09-26 Welltec A/S Système d'exécution de fond de trou

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2270188A3 (fr) * 2001-06-22 2011-09-07 Pioneer Hi-Bred International, Inc. Polynucléotides de défensine et procédés d'utilisation
US7256327B2 (en) * 2002-11-29 2007-08-14 The University Of Hong Kong Genetically modified plants expressing proteinase inhibitors, SaPIN2a or SaPIN2b, and methods of use thereof for the inhibition of trypsin- and chymotrypsin-like activities

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ANA BEATRIZ MORENO ET AL.: 'Pathogen-Induced Production of the Antifungal AFP Protein from Aspergillus giganteus Confers Resistance to the Blast Fungus Magnaporthe grisea in Transgenic Rice' MOLECULAR PLANT-MICROBE INTERACTIONS vol. 18, no. 9, 2005, pages 960 - 972 *
ANTONIO MOLINA ET AL.: 'Wheat Genes Encoding Two Types of PR-1 Proteins Are Pathogen Inducible, but Do Not Respond to Activators of Systemic Acquired Resistance' MOLECULAR PLANT-MICROBE INTERACTIONS vol. 12, no. 1, 1999, pages 53 - 58 *
DATABASE GENBANK 18 June 2003 Database accession no. BT008913 *
LU HUAFEI ET AL.: 'Construction of chimeric inducible promoters by elicitors of rice fungal blast pathogen and their expression in transgenic rice' CHINESE SCIENCE BULLETIN vol. 45, no. 3, February 2000, pages 242 - 246 *
TOMOKO IGAWA ET AL.: 'New TAXI-type Xylanase Inhibitor Genes are Inducible by Pathogens and Wounding in Hexaploid Wheat' PLANT AND CELL PHYSIOLOGY vol. 45, no. 10, 2004, pages 1347 - 1360 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012027209A3 (fr) * 2010-08-23 2012-07-05 Pioneer Hi-Bred International, Inc. Variants inédits de la défensine et leurs procédés d'utilisation

Also Published As

Publication number Publication date
WO2010059558A3 (fr) 2010-10-21
US20150106972A1 (en) 2015-04-16
US20100132070A1 (en) 2010-05-27
US20120124699A1 (en) 2012-05-17
CA2743770A1 (fr) 2010-05-27

Similar Documents

Publication Publication Date Title
US10457957B2 (en) Insecticidal proteins and methods of use
US6911577B2 (en) Defensin polynucleotides and methods of use
US7449552B2 (en) Bacillus thuringiensis cry gene and protein
US7329736B2 (en) Bacillus thuringiensis cry gene and protein
CA2981088A1 (fr) Genes pesticides et leurs procedes d'utilisation
MXPA06009411A (es) Lipasas y metodos de uso.
US8865967B2 (en) Defensin variants and methods of use
CA3222614A1 (fr) Genes pesticides et leurs procedes d'utilisation
KR20190045293A (ko) 살충 유전자 및 사용 방법
US20150106972A1 (en) Plant defense genes and proteins and methods of use
US20120054912A1 (en) Vacuole Targeting Peptides and Methods of Use
US20090011999A1 (en) Orally active androctonus amoreuxi pesticidal biopeptides
CA2724313C (fr) Polynucleotides de defensine et methodes d'utilisation
Class et al. Patent application title: NOVEL INSECTICIDAL PROTEINS AND METHODS OF USE
MX2013002236A (es) Peptidos dirigidos a vacuolas y metodos de uso.
PARKS et al. Patent 2970788 Summary

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 09828075

Country of ref document: EP

Kind code of ref document: A2

WWE Wipo information: entry into national phase

Ref document number: 2743770

Country of ref document: CA

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 09828075

Country of ref document: EP

Kind code of ref document: A2