WO2010059028A1 - Bactéries pour la dégradation et la modification de matières grasses, huiles et graisse - Google Patents
Bactéries pour la dégradation et la modification de matières grasses, huiles et graisse Download PDFInfo
- Publication number
- WO2010059028A1 WO2010059028A1 PCT/MY2008/000150 MY2008000150W WO2010059028A1 WO 2010059028 A1 WO2010059028 A1 WO 2010059028A1 MY 2008000150 W MY2008000150 W MY 2008000150W WO 2010059028 A1 WO2010059028 A1 WO 2010059028A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- bacillus
- grease
- composition
- strain
- ranging
- Prior art date
Links
- 241000894006 Bacteria Species 0.000 title claims description 6
- 239000004519 grease Substances 0.000 title abstract description 37
- 239000003921 oil Substances 0.000 title abstract description 24
- 239000003925 fat Substances 0.000 title abstract description 13
- 230000015556 catabolic process Effects 0.000 title description 9
- 238000006731 degradation reaction Methods 0.000 title description 9
- 230000004048 modification Effects 0.000 title description 2
- 238000012986 modification Methods 0.000 title description 2
- 239000000203 mixture Substances 0.000 claims abstract description 25
- 239000004094 surface-active agent Substances 0.000 claims description 9
- 241000193755 Bacillus cereus Species 0.000 claims description 8
- 241000194110 Bacillus sp. (in: Bacteria) Species 0.000 claims description 8
- 230000001580 bacterial effect Effects 0.000 claims description 7
- 238000009472 formulation Methods 0.000 claims description 7
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims description 6
- 239000003755 preservative agent Substances 0.000 claims description 6
- 241000194103 Bacillus pumilus Species 0.000 claims description 4
- 239000003205 fragrance Substances 0.000 claims description 4
- 235000015097 nutrients Nutrition 0.000 claims description 4
- 230000002335 preservative effect Effects 0.000 claims description 4
- 108090000790 Enzymes Proteins 0.000 claims description 3
- 102000004190 Enzymes Human genes 0.000 claims description 3
- 241001647055 uncultured Bacillus sp. Species 0.000 claims description 3
- 239000012190 activator Substances 0.000 claims description 2
- 231100000252 nontoxic Toxicity 0.000 claims description 2
- 230000003000 nontoxic effect Effects 0.000 claims description 2
- 239000003381 stabilizer Substances 0.000 claims description 2
- 239000000945 filler Substances 0.000 claims 1
- 244000005700 microbiome Species 0.000 abstract description 20
- 235000014113 dietary fatty acids Nutrition 0.000 abstract description 16
- 239000000194 fatty acid Substances 0.000 abstract description 16
- 229930195729 fatty acid Natural products 0.000 abstract description 16
- 150000004665 fatty acids Chemical class 0.000 abstract description 14
- 238000000034 method Methods 0.000 abstract description 8
- 230000000593 degrading effect Effects 0.000 abstract description 6
- 235000019198 oils Nutrition 0.000 description 22
- 241000193830 Bacillus <bacterium> Species 0.000 description 17
- 239000000047 product Substances 0.000 description 17
- 239000002351 wastewater Substances 0.000 description 11
- 230000008901 benefit Effects 0.000 description 9
- 235000013305 food Nutrition 0.000 description 7
- PEDCQBHIVMGVHV-UHFFFAOYSA-N glycerol group Chemical group OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 7
- 239000000463 material Substances 0.000 description 7
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 6
- 239000007788 liquid Substances 0.000 description 6
- 239000012263 liquid product Substances 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- -1 fatty acid salt Chemical class 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 238000011282 treatment Methods 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 101710158368 Extracellular lipase Proteins 0.000 description 4
- 101710128940 Triacylglycerol lipase Proteins 0.000 description 4
- 238000006065 biodegradation reaction Methods 0.000 description 4
- 230000001717 pathogenic effect Effects 0.000 description 4
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 3
- 108090001060 Lipase Proteins 0.000 description 3
- 102000004882 Lipase Human genes 0.000 description 3
- 239000004367 Lipase Substances 0.000 description 3
- 235000019482 Palm oil Nutrition 0.000 description 3
- 239000003139 biocide Substances 0.000 description 3
- 235000019421 lipase Nutrition 0.000 description 3
- 239000002540 palm oil Substances 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- 238000004065 wastewater treatment Methods 0.000 description 3
- FJKROLUGYXJWQN-UHFFFAOYSA-N 4-hydroxybenzoic acid Chemical compound OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 244000063299 Bacillus subtilis Species 0.000 description 2
- 235000014469 Bacillus subtilis Nutrition 0.000 description 2
- 241000193388 Bacillus thuringiensis Species 0.000 description 2
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 2
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 2
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 2
- FRHBOQMZUOWXQL-UHFFFAOYSA-L ammonium ferric citrate Chemical compound [NH4+].[Fe+3].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O FRHBOQMZUOWXQL-UHFFFAOYSA-L 0.000 description 2
- BTBJBAZGXNKLQC-UHFFFAOYSA-N ammonium lauryl sulfate Chemical compound [NH4+].CCCCCCCCCCCCOS([O-])(=O)=O BTBJBAZGXNKLQC-UHFFFAOYSA-N 0.000 description 2
- 229940063953 ammonium lauryl sulfate Drugs 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- DMSMPAJRVJJAGA-UHFFFAOYSA-N benzo[d]isothiazol-3-one Chemical compound C1=CC=C2C(=O)NSC2=C1 DMSMPAJRVJJAGA-UHFFFAOYSA-N 0.000 description 2
- 230000004071 biological effect Effects 0.000 description 2
- LLEMOWNGBBNAJR-UHFFFAOYSA-N biphenyl-2-ol Chemical compound OC1=CC=CC=C1C1=CC=CC=C1 LLEMOWNGBBNAJR-UHFFFAOYSA-N 0.000 description 2
- LLSDKQJKOVVTOJ-UHFFFAOYSA-L calcium chloride dihydrate Chemical compound O.O.[Cl-].[Cl-].[Ca+2] LLSDKQJKOVVTOJ-UHFFFAOYSA-L 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 239000013065 commercial product Substances 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 2
- GVGUFUZHNYFZLC-UHFFFAOYSA-N dodecyl benzenesulfonate;sodium Chemical compound [Na].CCCCCCCCCCCCOS(=O)(=O)C1=CC=CC=C1 GVGUFUZHNYFZLC-UHFFFAOYSA-N 0.000 description 2
- 239000003623 enhancer Substances 0.000 description 2
- 125000005313 fatty acid group Chemical group 0.000 description 2
- 229960004642 ferric ammonium citrate Drugs 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 239000004313 iron ammonium citrate Substances 0.000 description 2
- 235000000011 iron ammonium citrate Nutrition 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 description 2
- 229910052748 manganese Inorganic materials 0.000 description 2
- 239000011572 manganese Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- VAMXMNNIEUEQDV-UHFFFAOYSA-N methyl anthranilate Chemical compound COC(=O)C1=CC=CC=C1N VAMXMNNIEUEQDV-UHFFFAOYSA-N 0.000 description 2
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 239000011785 micronutrient Substances 0.000 description 2
- 235000013369 micronutrients Nutrition 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000012071 phase Substances 0.000 description 2
- 238000009428 plumbing Methods 0.000 description 2
- 235000010333 potassium nitrate Nutrition 0.000 description 2
- 239000004323 potassium nitrate Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 238000005086 pumping Methods 0.000 description 2
- 229940080264 sodium dodecylbenzenesulfonate Drugs 0.000 description 2
- 235000019333 sodium laurylsulphate Nutrition 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- 229940116368 1,2-benzisothiazoline-3-one Drugs 0.000 description 1
- XFRVVPUIAFSTFO-UHFFFAOYSA-N 1-Tridecanol Chemical compound CCCCCCCCCCCCCO XFRVVPUIAFSTFO-UHFFFAOYSA-N 0.000 description 1
- OLQJQHSAWMFDJE-UHFFFAOYSA-N 2-(hydroxymethyl)-2-nitropropane-1,3-diol Chemical compound OCC(CO)(CO)[N+]([O-])=O OLQJQHSAWMFDJE-UHFFFAOYSA-N 0.000 description 1
- KLFVDTRVIFNWIH-UHFFFAOYSA-N 2-[2-(2-tridecoxyethoxy)ethoxy]ethanol Chemical compound CCCCCCCCCCCCCOCCOCCOCCO KLFVDTRVIFNWIH-UHFFFAOYSA-N 0.000 description 1
- HUHGPYXAVBJSJV-UHFFFAOYSA-N 2-[3,5-bis(2-hydroxyethyl)-1,3,5-triazinan-1-yl]ethanol Chemical compound OCCN1CN(CCO)CN(CCO)C1 HUHGPYXAVBJSJV-UHFFFAOYSA-N 0.000 description 1
- WBIQQQGBSDOWNP-UHFFFAOYSA-N 2-dodecylbenzenesulfonic acid Chemical compound CCCCCCCCCCCCC1=CC=CC=C1S(O)(=O)=O WBIQQQGBSDOWNP-UHFFFAOYSA-N 0.000 description 1
- 229940100555 2-methyl-4-isothiazolin-3-one Drugs 0.000 description 1
- 229940090248 4-hydroxybenzoic acid Drugs 0.000 description 1
- PXRKCOCTEMYUEG-UHFFFAOYSA-N 5-aminoisoindole-1,3-dione Chemical compound NC1=CC=C2C(=O)NC(=O)C2=C1 PXRKCOCTEMYUEG-UHFFFAOYSA-N 0.000 description 1
- 229940100484 5-chloro-2-methyl-4-isothiazolin-3-one Drugs 0.000 description 1
- 241000589291 Acinetobacter Species 0.000 description 1
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 1
- 241000228212 Aspergillus Species 0.000 description 1
- 241000589941 Azospirillum Species 0.000 description 1
- 241000194108 Bacillus licheniformis Species 0.000 description 1
- 241000194107 Bacillus megaterium Species 0.000 description 1
- 239000005711 Benzoic acid Substances 0.000 description 1
- 241000149420 Bothrometopus brevis Species 0.000 description 1
- 241000589539 Brevundimonas diminuta Species 0.000 description 1
- 241000131418 Brevundimonas vesicularis Species 0.000 description 1
- LVDKZNITIUWNER-UHFFFAOYSA-N Bronopol Chemical compound OCC(Br)(CO)[N+]([O-])=O LVDKZNITIUWNER-UHFFFAOYSA-N 0.000 description 1
- 241001453380 Burkholderia Species 0.000 description 1
- 241000722910 Burkholderia mallei Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 239000004215 Carbon black (E152) Substances 0.000 description 1
- 241000146399 Ceriporiopsis Species 0.000 description 1
- GHXZTYHSJHQHIJ-UHFFFAOYSA-N Chlorhexidine Chemical compound C=1C=C(Cl)C=CC=1NC(N)=NC(N)=NCCCCCCN=C(N)N=C(N)NC1=CC=C(Cl)C=C1 GHXZTYHSJHQHIJ-UHFFFAOYSA-N 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- PHMNXPYGVPEQSJ-UHFFFAOYSA-N Dimethoxane Chemical compound CC1CC(OC(C)=O)OC(C)O1 PHMNXPYGVPEQSJ-UHFFFAOYSA-N 0.000 description 1
- QXNVGIXVLWOKEQ-UHFFFAOYSA-N Disodium Chemical compound [Na][Na] QXNVGIXVLWOKEQ-UHFFFAOYSA-N 0.000 description 1
- 241000588914 Enterobacter Species 0.000 description 1
- 241000588722 Escherichia Species 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 235000006679 Mentha X verticillata Nutrition 0.000 description 1
- 235000002899 Mentha suaveolens Nutrition 0.000 description 1
- 235000001636 Mentha x rotundifolia Nutrition 0.000 description 1
- 241000193397 Paenibacillus pabuli Species 0.000 description 1
- 241001057811 Paracoccus <mealybug> Species 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 241000589540 Pseudomonas fluorescens Species 0.000 description 1
- 241000589776 Pseudomonas putida Species 0.000 description 1
- 241000589615 Pseudomonas syringae Species 0.000 description 1
- 241000589625 Ralstonia pickettii Species 0.000 description 1
- 241000316848 Rhodococcus <scale insect> Species 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical class [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 241000605118 Thiobacillus Species 0.000 description 1
- 241000223259 Trichoderma Species 0.000 description 1
- 241000589634 Xanthomonas Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 125000000217 alkyl group Chemical group 0.000 description 1
- 239000008346 aqueous phase Substances 0.000 description 1
- 239000011668 ascorbic acid Substances 0.000 description 1
- 229940097012 bacillus thuringiensis Drugs 0.000 description 1
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 description 1
- 125000000484 butyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 229960003260 chlorhexidine Drugs 0.000 description 1
- DHNRXBZYEKSXIM-UHFFFAOYSA-N chloromethylisothiazolinone Chemical compound CN1SC(Cl)=CC1=O DHNRXBZYEKSXIM-UHFFFAOYSA-N 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Substances OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 239000013527 degreasing agent Substances 0.000 description 1
- SOROIESOUPGGFO-UHFFFAOYSA-N diazolidinylurea Chemical compound OCNC(=O)N(CO)C1N(CO)C(=O)N(CO)C1=O SOROIESOUPGGFO-UHFFFAOYSA-N 0.000 description 1
- 229960001083 diazolidinylurea Drugs 0.000 description 1
- ZBCBWPMODOFKDW-UHFFFAOYSA-N diethanolamine Chemical compound OCCNCCO ZBCBWPMODOFKDW-UHFFFAOYSA-N 0.000 description 1
- WSDISUOETYTPRL-UHFFFAOYSA-N dmdm hydantoin Chemical compound CC1(C)N(CO)C(=O)N(CO)C1=O WSDISUOETYTPRL-UHFFFAOYSA-N 0.000 description 1
- 229940060296 dodecylbenzenesulfonic acid Drugs 0.000 description 1
- SYELZBGXAIXKHU-UHFFFAOYSA-N dodecyldimethylamine N-oxide Chemical compound CCCCCCCCCCCC[N+](C)(C)[O-] SYELZBGXAIXKHU-UHFFFAOYSA-N 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 238000004945 emulsification Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- BEFDCLMNVWHSGT-UHFFFAOYSA-N ethenylcyclopentane Chemical compound C=CC1CCCC1 BEFDCLMNVWHSGT-UHFFFAOYSA-N 0.000 description 1
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000004133 fatty acid degradation Effects 0.000 description 1
- 239000002657 fibrous material Substances 0.000 description 1
- 238000004442 gravimetric analysis Methods 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 150000002430 hydrocarbons Chemical class 0.000 description 1
- 125000004356 hydroxy functional group Chemical group O* 0.000 description 1
- ZCTXEAQXZGPWFG-UHFFFAOYSA-N imidurea Chemical compound O=C1NC(=O)N(CO)C1NC(=O)NCNC(=O)NC1C(=O)NC(=O)N1CO ZCTXEAQXZGPWFG-UHFFFAOYSA-N 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 229940048866 lauramine oxide Drugs 0.000 description 1
- 239000012669 liquid formulation Substances 0.000 description 1
- 239000000314 lubricant Substances 0.000 description 1
- ZLNQQNXFFQJAID-UHFFFAOYSA-L magnesium carbonate Chemical compound [Mg+2].[O-]C([O-])=O ZLNQQNXFFQJAID-UHFFFAOYSA-L 0.000 description 1
- 239000001095 magnesium carbonate Substances 0.000 description 1
- 229910000021 magnesium carbonate Inorganic materials 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 229940102398 methyl anthranilate Drugs 0.000 description 1
- JWZXKXIUSSIAMR-UHFFFAOYSA-N methylene bis(thiocyanate) Chemical compound N#CSCSC#N JWZXKXIUSSIAMR-UHFFFAOYSA-N 0.000 description 1
- BEGLCMHJXHIJLR-UHFFFAOYSA-N methylisothiazolinone Chemical compound CN1SC=CC1=O BEGLCMHJXHIJLR-UHFFFAOYSA-N 0.000 description 1
- 239000012569 microbial contaminant Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- UYDLBVPAAFVANX-UHFFFAOYSA-N octylphenoxy polyethoxyethanol Chemical compound CC(C)(C)CC(C)(C)C1=CC=C(OCCOCCOCCOCCO)C=C1 UYDLBVPAAFVANX-UHFFFAOYSA-N 0.000 description 1
- 239000012074 organic phase Substances 0.000 description 1
- 235000010292 orthophenyl phenol Nutrition 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- DEUKGDDIXCQKES-UHFFFAOYSA-N phenol;sodium Chemical compound [Na].[Na].OC1=CC=CC=C1.OC1=CC=CC=C1 DEUKGDDIXCQKES-UHFFFAOYSA-N 0.000 description 1
- 125000001436 propyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- UKHVLWKBNNSRRR-TYYBGVCCSA-M quaternium-15 Chemical compound [Cl-].C1N(C2)CN3CN2C[N+]1(C/C=C/Cl)C3 UKHVLWKBNNSRRR-TYYBGVCCSA-M 0.000 description 1
- 229940096792 quaternium-15 Drugs 0.000 description 1
- 238000009991 scouring Methods 0.000 description 1
- 239000012056 semi-solid material Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 235000010294 sodium orthophenyl phenol Nutrition 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- 229940048842 sodium xylenesulfonate Drugs 0.000 description 1
- QUCDWLYKDRVKMI-UHFFFAOYSA-M sodium;3,4-dimethylbenzenesulfonate Chemical compound [Na+].CC1=CC=C(S([O-])(=O)=O)C=C1C QUCDWLYKDRVKMI-UHFFFAOYSA-M 0.000 description 1
- 239000002910 solid waste Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 235000010199 sorbic acid Nutrition 0.000 description 1
- 239000004334 sorbic acid Substances 0.000 description 1
- 229940075582 sorbic acid Drugs 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 229940087291 tridecyl alcohol Drugs 0.000 description 1
- 238000003911 water pollution Methods 0.000 description 1
- 210000002268 wool Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/343—Biological treatment of water, waste water, or sewage characterised by the microorganisms used for digestion of grease, fat, oil
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/085—Bacillus cereus
Definitions
- the invention discloses several Gram-positive microorganisms that effectively and efficiently degrade fats, oils and greases.
- a composition comprising said microorganism and a method for degrading fatty acids and grease are also disclosed. More particularly, the present invention is related to providing a non-pathogenic, spore-forming Gram-positive, lipophilic bacterial strain that produces extracellular lipase and also efficiently oxidizes or degrades fatty acids and grease.
- the invention is further related to a formulation comprising said Gram-positive organism.
- Fats, oils and greases (FOGs) in wastewater create problems including the production of foul odours, the blockage of sewer lines and may interfere with the proper operation of sewage treatment works. Removal of FOG from wastewater is thus critically important to ensure that wastewater is disposed of efficiently and economically.
- One of the major mechanisms of sewer line blockage due to oil is the conversion of oil to a greasy-like substance. When oil was incubated with the wastewater bacteria, an opaque, semi-solid sticky material was formed. Some of this material stuck to the sides of the culture vessels. The composition of the fatty acids was found to be different from the original oil.
- the frequency of pumping the accumulated grease solids can be quite variable, ranging from several weeks to several months. If traps are not cleaned on a regular basis, grease clogs may occur causing wastewater to back up into the food preparation area causing malodours and requiring the establishment to close until the problem is corrected.
- grease traps can function to biologically mediate a reduction of BOD and O&G in the bulk liquid resulting in cleaner effluent wastewater. This reduction of BOD and O&G is dependent upon the hydraulic retention time, which is dependent on the size of the grease trap and wastewater flow. Other factors that affect biological activity within a grease trap include pH, temperature and whether or not the facility practices bioaugmentation.
- Bioaugmentation the addition of commercial bacterial products that increase the biological activity in the system, has been used to reduce the BOD and O&G in the effluents from grease traps. This has helped to reduce surcharges that the food establishments must pay to municipalities for wastewater services. Additionally, bioaugmentation has been used to decrease the pumping frequency of grease traps, to keep drain lines open and to reduce malodours. In addition to grease traps, bioaugmentation has also been used to help remove grease from lift stations, drain lines, septic tanks and other situations where grease accumulation can cause flow problems and malodours. Bioaugmentation products can be either liquid or dry. Because of ease of handling, liquid products are generally preferred and added by a liquid metering pump drawing on a container that is replenished on a periodic basis.
- Gram-negative microorganisms When Gram-negative microorganisms are used for bioaugmentation in liquid products, they are present as vegetative cells and as such, they may be killed by chemicals, such as surfactants and preservatives, which are often used in such formulations. Therefore, products containing Gram-negative organisms cannot contain biocides and surfactants. Then, unpreserved liquid products may develop severe malodours from microbial contaminants growing in the product. Some of these contaminants may be undesirable in a food service environment. Furthermore, unpreserved products may also suffer from decreased shelf life and efficacy. Clearly, while Gram-negative microorganisms have an advantage in fatty acid degradation, their use in residential and food service products have serious drawbacks.
- Dry Gram-negative products do have a slight advantage of improved shelf life over liquid Gram-negative formulations. However, this advantage is only marginal and varies significantly from strain to strain. Disadvantages of dry products include contaminating dust and difficulty in handling dry materials. Although dry products can be rehydrated with water and applied like liquid products, the disadvantages of using unpreserved liquids containing Gram- negative microorganisms still apply to rehydrated dry materials. Many Gram-negative microorganisms are known to have the ability to biodegrade fatty acids generated by the action of lipase. This ability to oxidize and degrade fatty acids is generally not found in Gram-positive, spore-forming microorganisms, specifically members of the genus Bacillus.
- WO200294181-A by Tisinger J L, Paone D A, Leder J, Drahos DJ, Pacne DA, Brahos DJ, Tisinger J, Paone D, Drahos D, Tisinger L, Paone A and Drahos J (2003)- use a novel Bacillus megaterium SB3112 (A) having the ATCC deposit number PTA-3142 for degrading fatty acids and grease flowing into a sewer or to an on-site waste disposal system.
- JP2004009044-A by Ekomatekku Kenkyusho, Kaji Y, Takahashi M (2004) - using an oil absorbing-decomposing composition comprises cellulosic fibrous material; Bacillus subtilis, B.brevis, B.licheniformis, B.thuringiensis, B.cereus and/or B.tumilus and Pseudomonas aeruginosa, P.fluorescens, P.putida, P.syringae, P.mallei, P.diminuta, P.vesicularis and/or P.pickettii.
- Figure 1 the comparison of palm oil-degrading efficiency between the commercial product and individual strains of Bacillus spp. locally isolated
- Figure 2 is 16sRNA gene sequence for Bacillus cereus Strain Dr.Y135 with accession number of EF121823
- Figure 3 is 16sRNA gene sequence for Bacillus sp. strain SeAG 1 Y135 with accession number of EU828795
- Figure 4 is 16sRNA gene sequence for Uncultured Bacillus sp. clone A.rzi Y135 with accession number of EU835195
- Figure 5 is 16sRNA gene sequence for Bacillus amyloliquefaciens strain RXZ Y135 with accession number of EU835195
- an object of the present invention to provide a non-pathogenic, spore- forming Gram-positive, lipophilic bacterial strain that produces extracellular lipase and also efficiently hydrolyzes or degrades fatty acids and grease or a mixture of fatty acid and grease.
- An additional object of the present invention is to provide a method for degrading fatty acid and grease using a Gram-positive strain of Bacillus spp.
- Yet another object of the present invention is to enhance the biodegrading activity of Gram-positive strain of Bacillus species.
- the present invention relates to Gram positive microorganisms, Bacillus spp.
- the disclosed Bacillus species is effective for degradation of fats, oils and greases.
- the above and various other objects and advantages of the present invention are achieved by biologically pure cultures Gram-positive microorganism, Bacillus spp.
- the said microorganisms with their 16srRNA gene sequence submitted to Genbank with the accession numbers of EF121823, EU828795, EU835195 and EU835195.
- biodegradation means that the substrate is broken down, oxidized or degraded by the microorganism.
- activity enhancement means that the biodegradation activity of the microorganism is increased by the presence or addition of a particular component, said component being designated as “activity enhancer”, or “activator”.
- the preserved, spore-based Gram-positive containing product can contain preservatives and surfactants to aid in the biodegradation of fats, oils and greases (FOGs), because the spores are relatively resistant to biocides and surfactants. Furthermore, these products may also contain micronutrients promoting the growth of the microorganisms.
- a Gram-positive product comprising lipase-producing, fatty acid degrading, spore-forming microorganism in a preserved liquid formulation offers various advantages required for efficacious degradation of oil and grease.
- Liquid products formulated in accordance with the present invention for grease traps, or other similar uses where fatty acid or grease needs to be degraded may also contain in addition to surfactants, biocides, growth promoting non-toxic amounts of inorganic nutrients and micronutrients, certain activity enhancers, stabilizers, viscosifiers and the like.
- Acinetobacter Aspergillus, Azospirillum, Burkholderia, Bacillus, Ceriporiopsis,
- Rhodococcus Syphingomonas, Streptococcus, Thiobacillus, Trichoderma and
- the microorganism may be selected from the group consisting of Bacillus subtilis, Bacillus cereus, Bacillus pumilus, and Bacillus thuringiensis and a combination thereof.
- the preservative is selected from the group consisting of 1 ,2-benzisothiazolin-3- one;5-chloro-2-methyl-4-isothiazolin-3-one, 2-methyl-4-isothiazolin-3-one; quaternium-15; phenol; sodium o-phenylphenate; o-phenylphenol; 6-acetoxy-2,4- dimethyl-m-dioxane; tris(hydroxymethyl)nitromethane; hexahydro-1 ,3,5-tris(2- hydroxyethyl)-s-triazine; chlorhexidine; p-hydroxybenzoic acid or its methyl, ethyl, propyl, or butyl esters; benzoic, ascorbic, citric, or sorbic acid; imidazolidinyl urea; diazolidinyl urea; dimethylol dimethylhydantoin; methylene bisthiocyanate; 2-bromo- 2-nitropropyl
- the surfactant is selected from the group consisting of trideceth-3; 3 mole ethylene oxide adduct of a linear, primary C12-14 alcohol; 7 mole ethylene oxide adduct of a linear, primary C12-14 alcohol; sodium lauryl sulfate; ammonium lauryl sulfate; dodecyl benzene sulfonic acid; ammonium lauryl sulfate; sodium xylene sulfonate; sodium lauryl sulfate; cocamide diethanolamine; lauramine oxide; sodium alphasulfo methyl C12-18 ester and disodium alphasulfo C12-18 fatty acid salt; sodium dodecylbenzene sulfonate; alkyl polyglycoside; nonylphenoxypoly (ethyleneoxy) ethanol, branched; nonylphenoxypoly (ethyleneoxy) ethanol, branched; alkoxylated linear alcohol; blend of ethoxylate
- preservative ranging from 1 ppm to 1.0%
- color ranging from 0.02% to 1 %
- fragrance ranging from 0.02% to 1.0%
- a commercial oil-degrading supplement, Microblaze, in the form of block was initially chipped using sterile spatula to give approximately 1 cm3 block having an approximate spore count of 1 x 10 8 CFU bacteria and were directly added into the oil growth medium.
- Bacillus spp. strains were initially grown overnight at 3O 0 C on an orbital shaker (250 rpm) and were added into the flask to a final concentration of 1 x 10 8 CFU/ml.
- Controls were prepared in the same manner but without the addition of the supplement. The flasks were incubated for 21 or 28 days at 30 0 C with shaking at 130 rev/min before the remaining lipids were extracted. All treatments and controls were prepared in triplicate.
- Lipids were extracted by transferring the contents of the flasks into separating funnels and adding 40 ml of dichloromethane. The funnels were shaken vigorously, allowed to settle and the organic phase transferred to a florentine flask. If emulsification had occurred, separation of the two phases was achieved by centrifugation at 4500 g for 2-10 min assisted by the addition of a few drops of saturated sodium chloride solution. The remaining aqueous phase was re-extracted a further 3 times and the solvent phases pooled together and dried using a rotary evaporator.
- the dried oil was redissolved in a few ml of dichloromethane, transferred to a vial containing anhydrous sodium sulphate and then filtered through Whatman number 6 filter paper into a pre-weighed vial.
- the solvent was evaporated under oxygen free nitrogen at 40°C and the weight of oil determined.
- Bacillus amyloliquefaciens strain RXZ (EU835195), Bacillus pumilus Strain Lubn2 (EU851976), Bacillus cereus Strain DRY135 (DQ 851857), Bacillus sp. strain SeAG 1 (EU828795) were obtained from the Dept. of Biochemistry Culture Collection, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia. Values in parentheses indicate Genbank Accession number. Benchmarking product, BioAccess, was obtained from and contained several undiclosed Bacillus strains;
- BioAccess product properties are as follow:
- Figure 1 showed the comparison of palm oil-degrading efficiency between the commercial product (BioAccess) product and individual strains of Bacillus spp. locally isolated. The degradation was carried out at 30 0 C since this is the normal environmental temperatures of Malaysian climate. Only Bacillus sp. strain SeAG 1 gave significantly higher reduction in palm oil than the commercial Bioaccess consortium (p ⁇ 0.05). The rest of the strains gave no significant difference in terms of palm oil degradation capacity compared to the commercial BioAccess consortium (p>0.05). However, the combination of all of the local strains gave significantly higher degradation of palm oil compared to the commercial BioAccess consortium (p ⁇ 0.05).
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Water Supply & Treatment (AREA)
- Biodiversity & Conservation Biology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Environmental & Geological Engineering (AREA)
- Hydrology & Water Resources (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
L'invention porte sur plusieurs microorganismes à Gram-positif qui dégradent de façon effective et efficace les matières grasses, les huiles et la graisse. L'invention porte sur une composition comprenant ledit microorganisme et sur un procédé pour dégrader des acides gras et la graisse.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/MY2008/000150 WO2010059028A1 (fr) | 2008-11-21 | 2008-11-21 | Bactéries pour la dégradation et la modification de matières grasses, huiles et graisse |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/MY2008/000150 WO2010059028A1 (fr) | 2008-11-21 | 2008-11-21 | Bactéries pour la dégradation et la modification de matières grasses, huiles et graisse |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2010059028A1 true WO2010059028A1 (fr) | 2010-05-27 |
Family
ID=40847841
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/MY2008/000150 WO2010059028A1 (fr) | 2008-11-21 | 2008-11-21 | Bactéries pour la dégradation et la modification de matières grasses, huiles et graisse |
Country Status (1)
Country | Link |
---|---|
WO (1) | WO2010059028A1 (fr) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP3187579A4 (fr) * | 2014-08-25 | 2017-07-26 | Japan Environmental Science Company | Composition, support, système de traitement des eaux usées, procédé de traitement des eaux usées, procédé de désodorisation, et procédé de traitement des eaux usées par lot |
FR3068688A1 (fr) * | 2017-07-05 | 2019-01-11 | Exochems Environnement | Composition bacterienne et utilisation pour le traitement des eaux usees et le traitement des dechets |
FR3115293A1 (fr) * | 2020-10-16 | 2022-04-22 | Open Medical System | Composition liquide de bactéries et procédé de conservation de bactéries |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6171847B1 (en) * | 1999-04-14 | 2001-01-09 | Roebic Laboratories, Inc. | Enzyme-producing strain of Bacillus bacteria |
WO2002094181A2 (fr) * | 2001-05-18 | 2002-11-28 | Novozymes A/S | Agent gram-positif de degradation des acides gras |
-
2008
- 2008-11-21 WO PCT/MY2008/000150 patent/WO2010059028A1/fr active Application Filing
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6171847B1 (en) * | 1999-04-14 | 2001-01-09 | Roebic Laboratories, Inc. | Enzyme-producing strain of Bacillus bacteria |
WO2002094181A2 (fr) * | 2001-05-18 | 2002-11-28 | Novozymes A/S | Agent gram-positif de degradation des acides gras |
Non-Patent Citations (1)
Title |
---|
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 2001, ARCHAMBAULT J G ET AL: "Development of a Bacillus consortium for digestion of food waste in grease trap system", XP002537501, Database accession no. PREV200200251470 * |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP3187579A4 (fr) * | 2014-08-25 | 2017-07-26 | Japan Environmental Science Company | Composition, support, système de traitement des eaux usées, procédé de traitement des eaux usées, procédé de désodorisation, et procédé de traitement des eaux usées par lot |
US10052399B2 (en) | 2014-08-25 | 2018-08-21 | Japan Environmental Science Company | Composition, support, wastewater treatment system, wastewater treating method, deodorization method, and batch wastewater treating method |
FR3068688A1 (fr) * | 2017-07-05 | 2019-01-11 | Exochems Environnement | Composition bacterienne et utilisation pour le traitement des eaux usees et le traitement des dechets |
FR3115293A1 (fr) * | 2020-10-16 | 2022-04-22 | Open Medical System | Composition liquide de bactéries et procédé de conservation de bactéries |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CA2447749C (fr) | Agent gram-positif de degradation des acides gras | |
AU2002303532A1 (en) | Gram-positive fatty acid degrader | |
US20090324533A1 (en) | Bacillus amyloliquefaciens Strain | |
JP4566207B2 (ja) | 油脂分解性微生物及びそれを用いた油脂含有廃水の処理方法 | |
Millán et al. | A study on the microbiota from olive-mill wastewater (OMW) disposal lagoons, with emphasis on filamentous fungi and their biodegradative potential | |
WO2010059028A1 (fr) | Bactéries pour la dégradation et la modification de matières grasses, huiles et graisse | |
RU2509150C2 (ru) | Ассоциация штаммов бактерий-нефтедеструкторов и способ ремедиации нефтезагрязненных объектов | |
JP2010214310A (ja) | 油脂分解能を有する微生物及びそれを用いた油脂含有排水の処理方法 | |
Coulon et al. | Effects of Biostimulation on Growth of Indigenous Bacteria in Sub-Antarctic Soil Contamined with Oil Hydrocarbons | |
Ma’an et al. | Isolation of bacterial strain for biodegradation of fats, oil and grease | |
CN101886041A (zh) | 一种高效处理城市湖水污染的微生物制剂 | |
Ben Hamed et al. | Efficiency of refinery sludge biodegradation using municipal wastewater and activated sludge and effect of hydrocarbon concentration on culturable bacteria community | |
US6762047B2 (en) | Bacterial parts washer, composition and method of use | |
WO2008020818A1 (fr) | Formulation microbienne granulaire capable d'auto-agrégation, utilisée dans le traitement des eaux résiduelles. | |
GASNER | Microorganisms for waste treatment | |
EP1025052B1 (fr) | Procede permettant de reduire l'action de detergents lors de la germination et/ou croissance de micro-organismes | |
JP2003038169A (ja) | グラム陽性脂肪酸分解微生物 | |
Tzirita et al. | An investigation of mixed microbial populations for use in the treatment of waste fats, oils and greases | |
Njalam'mano et al. | Isolation, identification and characterisation of butyric acid degrading bacterium from pit latrine faecal sludge | |
Wokem et al. | Bioremediation Of Palm Oil Mill Effluent (POME) Contaminated Soil Using Cow Dung. | |
Bharathi | Biodegradation of Crude Oil Contaminated Soil by Microbial Inoculants | |
Jilani | The influence of dissolved oxygen on growth and degradation of Cypermethrin by Pseudomonas using a biosimulator | |
Sen et al. | Potential application of Bacillus pseudofirmus SVB1 extract in effluent treatment | |
Eniolorunda et al. | Microbial bioremediation of selected wastewaters–A review | |
Boszczyk-Maleszak et al. | Biodegradation of petroleum products by microorganisms adapted to high crude oil concentration in presence of easy assimilated carbon source |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 08876199 Country of ref document: EP Kind code of ref document: A1 |
|
NENP | Non-entry into the national phase |
Ref country code: DE |
|
122 | Ep: pct application non-entry in european phase |
Ref document number: 08876199 Country of ref document: EP Kind code of ref document: A1 |