WO2010055362A3 - Method and measuring system for scanning a region of interest - Google Patents

Method and measuring system for scanning a region of interest Download PDF

Info

Publication number
WO2010055362A3
WO2010055362A3 PCT/HU2009/000095 HU2009000095W WO2010055362A3 WO 2010055362 A3 WO2010055362 A3 WO 2010055362A3 HU 2009000095 W HU2009000095 W HU 2009000095W WO 2010055362 A3 WO2010055362 A3 WO 2010055362A3
Authority
WO
WIPO (PCT)
Prior art keywords
scanning
interest
region
trajectories
providing
Prior art date
Application number
PCT/HU2009/000095
Other languages
French (fr)
Other versions
WO2010055362A2 (en
Inventor
BaIázs RÓZSA
Gergely Katona
E. Szilveszter Vizi
Original Assignee
Femtonics Kft.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from HU0800690A external-priority patent/HU0800690D0/en
Priority claimed from HU0800782A external-priority patent/HU0800782D0/en
Application filed by Femtonics Kft. filed Critical Femtonics Kft.
Publication of WO2010055362A2 publication Critical patent/WO2010055362A2/en
Publication of WO2010055362A3 publication Critical patent/WO2010055362A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V10/00—Arrangements for image or video recognition or understanding
    • G06V10/10—Image acquisition
    • G06V10/12—Details of acquisition arrangements; Constructional details thereof
    • G06V10/14—Optical characteristics of the device performing the acquisition or on the illumination arrangements
    • G06V10/147—Details of sensors, e.g. sensor lenses
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G06V20/693—Acquisition
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V30/00—Character recognition; Recognising digital ink; Document-oriented image-based pattern recognition
    • G06V30/10—Character recognition
    • G06V30/14—Image acquisition
    • G06V30/144—Image acquisition using a slot moved over the image; using discrete sensing elements at predetermined points; using automatic curve following means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/10—Scanning
    • G01N2201/105—Purely optical scan
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0036—Scanning details, e.g. scanning stages
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B26/00—Optical devices or arrangements for the control of light using movable or deformable optical elements
    • G02B26/08—Optical devices or arrangements for the control of light using movable or deformable optical elements for controlling the direction of light
    • G02B26/10—Scanning systems

Landscapes

  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Multimedia (AREA)
  • Theoretical Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Optics & Photonics (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Vascular Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microscoopes, Condenser (AREA)
  • Length Measuring Devices By Optical Means (AREA)

Abstract

The invention relates to a method for scanning a region of interest, such as a portion of a neural process, via a laser scanning microscope having focusing means for focusing a laser beam and having electro-mechano-optic deflecting means for deflecting the laser beam, the method comprising: providing a primary scanning trajectory (92a) for the at least one region of interest; providing a plurality of spaced apart (89) auxiliary scanning trajectories (92b) running along the primary scanning trajectory within the region of interest; providing a scanning sequence for scanning the scanning trajectories (192); providing cross-over (94) trajectories between the scanning trajectories of two consecutive scanning trajectories in the scanning sequence. The invention further relates to a measuring system for implementing the method according to the invention.
PCT/HU2009/000095 2008-11-17 2009-11-17 Method and measuring system for scanning a region of interest WO2010055362A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
HUP0800690 2008-11-17
HU0800690A HU0800690D0 (en) 2008-11-17 2008-11-17 Method for scanning folded frame
HUP0800782 2008-12-31
HU0800782A HU0800782D0 (en) 2008-12-31 2008-12-31 Method and measuring system for scanning a region of interest

Publications (2)

Publication Number Publication Date
WO2010055362A2 WO2010055362A2 (en) 2010-05-20
WO2010055362A3 true WO2010055362A3 (en) 2010-07-08

Family

ID=89988691

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/HU2009/000095 WO2010055362A2 (en) 2008-11-17 2009-11-17 Method and measuring system for scanning a region of interest

Country Status (1)

Country Link
WO (1) WO2010055362A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6163498B2 (en) 2011-12-28 2017-07-12 フェムトニクス ケイエフティー．Ｆｅｍｔｏｎｉｃｓ Ｋｆｔ． Method and system for three-dimensional measurement of sample by measurement system equipped with laser scanning microscope
ES2897575T3 (en) * 2013-06-03 2022-03-01 Lumicks Dsm Holding B V Method and system for imaging a molecular strand
HUE064140T2 (en) * 2016-09-02 2024-03-28 Femtonics Kft Method for scanning along a 3-dimensional line and method for scanning a region of interest by scanning a plurality of 3-dimensional lines
CN106596410A (en) * 2016-11-22 2017-04-26 西安工程大学 Method for quickly detecting property of active sludge through light deflection
US10690486B2 (en) * 2018-07-03 2020-06-23 Amo Development, Llc Water-immersed high precision laser focus spot size measurement apparatus

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020027203A1 (en) * 2000-09-05 2002-03-07 Johann Engelhardt Method for examining a specimen, and confocal scanning microscope
US20060011804A1 (en) * 2004-07-16 2006-01-19 Ralf Engelmann Process for the observation of at least one sample region with a light raster microscope with linear sampling
EP1882967A1 (en) * 2005-05-16 2008-01-30 Olympus Corporation Scanning type observing device

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020027203A1 (en) * 2000-09-05 2002-03-07 Johann Engelhardt Method for examining a specimen, and confocal scanning microscope
US20060011804A1 (en) * 2004-07-16 2006-01-19 Ralf Engelmann Process for the observation of at least one sample region with a light raster microscope with linear sampling
EP1882967A1 (en) * 2005-05-16 2008-01-30 Olympus Corporation Scanning type observing device

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DENK W ET AL: "Anatomical and functional imaging of neurons using 2-photon laser scanning microscopy", JOURNAL OF NEUROSCIENCE METHODS, ELSEVIER SCIENCE PUBLISHER B.V., AMSTERDAM, NL LNKD- DOI:10.1016/0165-0270(94)90189-9, vol. 54, no. 2, 1 October 1994 (1994-10-01), pages 151 - 162, XP022720475, ISSN: 0165-0270, [retrieved on 19941001] *

Also Published As

Publication number Publication date
WO2010055362A2 (en) 2010-05-20

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