WO2010041230A3 - Microfluidic integrated device for sample processing - Google Patents

Microfluidic integrated device for sample processing Download PDF

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Publication number
WO2010041230A3
WO2010041230A3 PCT/IB2009/055206 IB2009055206W WO2010041230A3 WO 2010041230 A3 WO2010041230 A3 WO 2010041230A3 IB 2009055206 W IB2009055206 W IB 2009055206W WO 2010041230 A3 WO2010041230 A3 WO 2010041230A3
Authority
WO
WIPO (PCT)
Prior art keywords
microchannel
integrated device
sample processing
microfluidic integrated
microfluidic
Prior art date
Application number
PCT/IB2009/055206
Other languages
French (fr)
Other versions
WO2010041230A2 (en
Inventor
Jean-Louis Viovy
Anne Le Nel
Original Assignee
Cnrs-Dae
Institut Curie
Universite Pierre Et Marie Curie (Paris 6)
Universite Paris Sud Xi
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cnrs-Dae, Institut Curie, Universite Pierre Et Marie Curie (Paris 6), Universite Paris Sud Xi filed Critical Cnrs-Dae
Priority to EP09807632A priority Critical patent/EP2346607A2/en
Publication of WO2010041230A2 publication Critical patent/WO2010041230A2/en
Publication of WO2010041230A3 publication Critical patent/WO2010041230A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G01N33/54333—Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/005—Pretreatment specially adapted for magnetic separation
    • B03C1/01—Pretreatment specially adapted for magnetic separation by addition of magnetic adjuvants
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/02—Magnetic separation acting directly on the substance being separated
    • B03C1/28—Magnetic plugs and dipsticks
    • B03C1/288—Magnetic plugs and dipsticks disposed at the outer circumference of a recipient
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C2201/00—Details of magnetic or electrostatic separation
    • B03C2201/20—Magnetic separation whereby the particles to be separated are in solid form
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C2201/00—Details of magnetic or electrostatic separation
    • B03C2201/26—Details of magnetic or electrostatic separation for use in medical applications

Abstract

An integrated microsystem (2), comprising: a microchannel (1), a field generator (9) to create a magnetic field in at least one first portion (5) of the microchannel having a direction substantially collinear with the direction of flow in the portion of the microchannel, the magnetic field also presenting a gradient, wherein the microsystem additionally comprises a detection area (7) in fluid connection with the microchannel. A method is also described in which magnetic microparticles are used to intersect with an analyte in the device.
PCT/IB2009/055206 2008-10-10 2009-10-12 Microfluidic integrated device for sample processing WO2010041230A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP09807632A EP2346607A2 (en) 2008-10-10 2009-10-12 Microfluidic integrated device for sample processing

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10451408P 2008-10-10 2008-10-10
US61/104,514 2008-10-10

Publications (2)

Publication Number Publication Date
WO2010041230A2 WO2010041230A2 (en) 2010-04-15
WO2010041230A3 true WO2010041230A3 (en) 2010-06-17

Family

ID=42101029

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2009/055206 WO2010041230A2 (en) 2008-10-10 2009-10-12 Microfluidic integrated device for sample processing

Country Status (2)

Country Link
EP (1) EP2346607A2 (en)
WO (1) WO2010041230A2 (en)

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* Cited by examiner, † Cited by third party
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US8765832B2 (en) 2011-10-14 2014-07-01 Exxonmobil Chemical Patents Inc. Polyolefin-based crosslinked compositions and methods of making them
GB2477506B (en) * 2010-02-03 2013-10-30 Dna Electronics Ltd Integrated electrochemical and optical sensor with inductor
KR101519379B1 (en) * 2010-04-29 2015-05-12 삼성전자 주식회사 Centrifugal Micro-fluidic Device and Method for immunoassay
DE102012210457B4 (en) * 2012-06-21 2015-08-27 Siemens Aktiengesellschaft Method and device for partial labeling and subsequent quantification of cells of a cell suspension
BR112014032304B1 (en) * 2012-06-22 2022-05-10 Zoetis Denmark Aps Method and system for the quantitative or qualitative determination of a target component in a liquid sample, and microfluidic device
FR2995225B1 (en) * 2012-09-07 2014-10-03 Jean-Louis Viovy MICROFLUIDIC SYSTEM HAVING A BED OF MAGNETIC PARTICLES
US9097710B2 (en) * 2012-10-16 2015-08-04 The Methodist Hospital Research Institute Multiplexed volumetric bar chart chip for point of care biomarker and analyte quantitation
US9835623B2 (en) * 2014-01-15 2017-12-05 Caliper Life Sciences, Inc. Method and system of microfluidic immunoassay using magnetic beads
US10031348B2 (en) 2014-10-15 2018-07-24 California Institute Of Technology Contact lens with metered liquid system
WO2017070528A1 (en) 2015-10-23 2017-04-27 California Institute Of Technology Reliable deposition of thin parylene
US10807093B2 (en) 2016-02-05 2020-10-20 Katholieke Universiteit Leuven Microfluidic systems
CN107478837B (en) * 2017-09-01 2018-12-14 北京华科泰生物技术有限公司 Micro-fluidic chemiluminescence detection system and its application based on magnetic particle
CN107702967B (en) * 2017-10-17 2019-11-22 西北工业大学 It is a kind of based on the space station of micro-fluidic chip cell sample automatic pretreatment apparatus
WO2019226682A1 (en) * 2018-05-22 2019-11-28 California Institute Of Technology Miniature fixed and adjustable flow restrictor for the body
MX2020012342A (en) 2018-06-11 2021-02-26 California Inst Of Techn Eye treatment device having a ring like shape.
CN109550528B (en) * 2018-12-14 2020-11-27 北京工业大学 Multi-angle nondestructive observation method and device for internal channel of micro-fluidic chip

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002093125A2 (en) * 2001-05-11 2002-11-21 President And Fellows Of Harvard College Micromagnetic systems and methods for microfluidics
US20030044832A1 (en) * 1996-09-04 2003-03-06 Scandinavian Micro Biodevices A/S Microflow system for particle separation and analysis
US20040018611A1 (en) * 2002-07-23 2004-01-29 Ward Michael Dennis Microfluidic devices for high gradient magnetic separation
US20060011552A1 (en) * 2004-06-25 2006-01-19 Canon Kabushiki Kaisha Apparatus and method for separating magnetic particles
WO2008094198A2 (en) * 2006-07-28 2008-08-07 Biosite Incorporated Devices and methods for performing receptor binding assays using magnetic particles

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030044832A1 (en) * 1996-09-04 2003-03-06 Scandinavian Micro Biodevices A/S Microflow system for particle separation and analysis
WO2002093125A2 (en) * 2001-05-11 2002-11-21 President And Fellows Of Harvard College Micromagnetic systems and methods for microfluidics
US20040018611A1 (en) * 2002-07-23 2004-01-29 Ward Michael Dennis Microfluidic devices for high gradient magnetic separation
US20060011552A1 (en) * 2004-06-25 2006-01-19 Canon Kabushiki Kaisha Apparatus and method for separating magnetic particles
WO2008094198A2 (en) * 2006-07-28 2008-08-07 Biosite Incorporated Devices and methods for performing receptor binding assays using magnetic particles

Also Published As

Publication number Publication date
EP2346607A2 (en) 2011-07-27
WO2010041230A2 (en) 2010-04-15

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