WO2010036939A3 - A system for synergistic expression of multiple small functional rna elements - Google Patents

A system for synergistic expression of multiple small functional rna elements Download PDF

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Publication number
WO2010036939A3
WO2010036939A3 PCT/US2009/058451 US2009058451W WO2010036939A3 WO 2010036939 A3 WO2010036939 A3 WO 2010036939A3 US 2009058451 W US2009058451 W US 2009058451W WO 2010036939 A3 WO2010036939 A3 WO 2010036939A3
Authority
WO
WIPO (PCT)
Prior art keywords
multiple small
functional rna
rna elements
small functional
synergistic expression
Prior art date
Application number
PCT/US2009/058451
Other languages
French (fr)
Other versions
WO2010036939A2 (en
Inventor
Jeffrey J. Friedman
Gangning Liang
Peter A. Jones
Original Assignee
University Of Southern California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2009/053203 external-priority patent/WO2010017510A1/en
Application filed by University Of Southern California filed Critical University Of Southern California
Publication of WO2010036939A2 publication Critical patent/WO2010036939A2/en
Publication of WO2010036939A3 publication Critical patent/WO2010036939A3/en
Priority to US13/296,121 priority Critical patent/US20120208267A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1135—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against oncogenes or tumor suppressor genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C12N2310/141—MicroRNAs, miRNAs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/50—Biochemical production, i.e. in a transformed host cell
    • C12N2330/51—Specially adapted vectors

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

The present invention relates in general to microRNAs (miRNAs). More specifically, the invention relates to expression vectors comprising multiple miRNAs or families and clusters capable of targeting multiple oncogenic pathways.
PCT/US2009/058451 2008-08-07 2009-09-25 A system for synergistic expression of multiple small functional rna elements WO2010036939A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US13/296,121 US20120208267A1 (en) 2008-08-07 2011-11-14 System for Synergistic Expression of Multiple Small Functional RNA Elements

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US10064608P 2008-09-26 2008-09-26
US61/100,646 2008-09-26
USPCT/US2009/053203 2009-08-07
PCT/US2009/053203 WO2010017510A1 (en) 2008-08-07 2009-08-07 A system for synergistic expression of multiple small functional rna elements

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/053203 Continuation-In-Part WO2010017510A1 (en) 2008-08-07 2009-08-07 A system for synergistic expression of multiple small functional rna elements

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US201113072576A Continuation 2008-08-07 2011-03-25

Publications (2)

Publication Number Publication Date
WO2010036939A2 WO2010036939A2 (en) 2010-04-01
WO2010036939A3 true WO2010036939A3 (en) 2010-06-17

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/058451 WO2010036939A2 (en) 2008-08-07 2009-09-25 A system for synergistic expression of multiple small functional rna elements

Country Status (1)

Country Link
WO (1) WO2010036939A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5587777B2 (en) 2007-07-31 2014-09-10 ボード・オブ・リージエンツ，ザ・ユニバーシテイ・オブ・テキサス・システム MicroRNA family modulating fibrosis and uses thereof
BRPI0818901A2 (en) 2007-11-09 2015-05-12 Univ Texas Mir-15 family micro-rna modulate cardiomyocyte survival and cardiac repair
EP2582399A4 (en) * 2010-06-16 2015-04-15 Minerva Biotechnologies Corp Reprogramming cancer cells
GB201117482D0 (en) * 2011-10-11 2011-11-23 Univ Dundee Targetiing of miRNA precursors
US20150140591A1 (en) 2012-06-06 2015-05-21 Boehringer Ingelheim International Gmbh CELL ENGINEERING USING RNAs
US9163235B2 (en) 2012-06-21 2015-10-20 MiRagen Therapeutics, Inc. Inhibitors of the miR-15 family of micro-RNAs
EP3788141A4 (en) * 2018-04-30 2023-03-08 The Brigham and Women's Hospital, Inc. Compositions and therapeutic methods of microrna gene delivery
KR102312393B1 (en) * 2021-04-09 2021-10-15 쎄니테크코리아(주) Composition for disinfection and sterilization comprising natural extracts
CN114010786A (en) * 2021-11-25 2022-02-08 江南大学 Method for treating triple negative breast cancer by double antisense nucleic acid synergistic photodynamic

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007033023A2 (en) * 2005-09-12 2007-03-22 The Ohio State University Research Foundation Compositions and methods for the diagnosis and therapy of bcl2-associated cancers
WO2008068046A1 (en) * 2006-12-05 2008-06-12 Istituto Superiore di Sanità Micro rna targeting aml1

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007033023A2 (en) * 2005-09-12 2007-03-22 The Ohio State University Research Foundation Compositions and methods for the diagnosis and therapy of bcl2-associated cancers
WO2008068046A1 (en) * 2006-12-05 2008-06-12 Istituto Superiore di Sanità Micro rna targeting aml1

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CIMMINO AMELIA ET AL: "miR-15 and miR-16 induce apoptosis by targeting BCL2", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE, WASHINGTON, DC, US, vol. 102, no. 39, 27 September 2005 (2005-09-27), pages 13944 - 13949, XP002484308, ISSN: 0027-8424 *
LE SAGE CARLOS ET AL: "Regulation of the p27(Kip1) tumor suppressor by miR-221 and miR-222 promotes cancer cell proliferation", EMBO (EUROPEAN MOLECULAR BIOLOGY ORGANIZATION) JOURNAL, vol. 26, no. 15, August 2007 (2007-08-01), pages 3699 - 3708, XP002550796, ISSN: 0261-4189 *
VELU CHINAVENMENI S ET AL: "Gfi1 regulates miR-21 and miR-196b to control myelopoiesis", BLOOD, vol. 113, no. 19, May 2009 (2009-05-01), pages 4720 - 4728, XP002550795, ISSN: 0006-4971 *
VOORHOEVE P MATHIJS ET AL: "A genetic screen implicates miRNA-372 and miRNA-373 as oncogenes in testicular germ cell tumors", CELL, vol. 124, no. 6, March 2006 (2006-03-01), pages 1169 - 1181, XP002550797, ISSN: 0092-8674 *

Also Published As

Publication number Publication date
WO2010036939A2 (en) 2010-04-01

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