WO2010036880A3 - Selection system for di-iron [fefe] hydrogenase properties - Google Patents

Selection system for di-iron [fefe] hydrogenase properties Download PDF

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Publication number
WO2010036880A3
WO2010036880A3 PCT/US2009/058361 US2009058361W WO2010036880A3 WO 2010036880 A3 WO2010036880 A3 WO 2010036880A3 US 2009058361 W US2009058361 W US 2009058361W WO 2010036880 A3 WO2010036880 A3 WO 2010036880A3
Authority
WO
WIPO (PCT)
Prior art keywords
properties
hydrogenase
fefe
iron
selection system
Prior art date
Application number
PCT/US2009/058361
Other languages
French (fr)
Other versions
WO2010036880A2 (en
Inventor
Edwin H. Wintermute
Christina Agapakis
Patrick Boyle
Pamela A. Silver
Original Assignee
President And Fellows Of Harvard College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by President And Fellows Of Harvard College filed Critical President And Fellows Of Harvard College
Publication of WO2010036880A2 publication Critical patent/WO2010036880A2/en
Publication of WO2010036880A3 publication Critical patent/WO2010036880A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0067—Oxidoreductases (1.) acting on hydrogen as donor (1.12)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The present invention provides engineered microorganisms such as E.coli and methods for selecting for desired biological properties of hydrogenase enzymes in vivo. Using the methods of the invention, hydrogenase enzymes are selected for properties including, but not limited to, oxygen tolerance, thermostability, and increased catalytic rate.
PCT/US2009/058361 2008-09-25 2009-09-25 Selection system for di-iron [fefe] hydrogenase properties WO2010036880A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US19415508P 2008-09-25 2008-09-25
US61/194,155 2008-09-25

Publications (2)

Publication Number Publication Date
WO2010036880A2 WO2010036880A2 (en) 2010-04-01
WO2010036880A3 true WO2010036880A3 (en) 2010-07-29

Family

ID=42060396

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/058361 WO2010036880A2 (en) 2008-09-25 2009-09-25 Selection system for di-iron [fefe] hydrogenase properties

Country Status (1)

Country Link
WO (1) WO2010036880A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20210147990A1 (en) * 2018-04-12 2021-05-20 Alliance For Sustainable Energy, Llc In vitro production of hydrogen utilizing hydrogenase

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004093524A2 (en) * 2003-04-18 2004-11-04 Midwest Research Institute Oxygen-resistant hydrogenases and methods for designing and making same
WO2006093998A2 (en) * 2005-02-28 2006-09-08 Midwest Research Institute Process and genes for expression and over-expression of active [fefe] hydrogenases
US20070009942A1 (en) * 2003-04-12 2007-01-11 Harrison Dillon Methods and compositions for evolving hydrogenase genes

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070009942A1 (en) * 2003-04-12 2007-01-11 Harrison Dillon Methods and compositions for evolving hydrogenase genes
WO2004093524A2 (en) * 2003-04-18 2004-11-04 Midwest Research Institute Oxygen-resistant hydrogenases and methods for designing and making same
WO2006093998A2 (en) * 2005-02-28 2006-09-08 Midwest Research Institute Process and genes for expression and over-expression of active [fefe] hydrogenases

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JAYSHREE MISHRA ET AL.: "Molecular cloning, characterization, and overexpression of a novel [Fe]-hydrogenase isolated from a high rate of htydrogen producing Enterobacter cloacae IIT-BT 08.", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 324, 2004, pages 679 - 685 *
PAUL W. KING ET AL.: "Functional Studies of [FeFe] Hydrogenase Maturation in an Escherichia coli Biosynthetic system.", JOURNAL OF BACTERIOLOGY, vol. 188, no. 6, March 2006 (2006-03-01), pages 2163 - 2172 *

Also Published As

Publication number Publication date
WO2010036880A2 (en) 2010-04-01

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