WO2010035946A1 - Composition containing acanthopanax extract as active ingredient for treatment and prevention of gastrointestinal disorders - Google Patents

Abstract

The present invention relates to a composition containing Acanthopanax extract as an active ingredient for treatment and prevention of gastrointestinal disorders selected from the group of gastritis, and gastric and duodenal ulcers. The Acanthopanax extract of the present invention has excellent effects of preventing and treating gastrointestinal damage caused by both chemical factors such as anhydrous ethanol or cysteamine and physical factors such as stress. Also, the Acanthopanax extract has an excellent inhibitory effect on Helicobacter pylori by significantly suppressing urease activity and the growth of Helicobacter pylori. Accordingly, the Acanthopanax extract according to the present invention can be useful in treatment and prevention of gastrointestinal disorder such as gastritis, and gastric and duodenal ulcers.

Description

Composition for the prevention or treatment of gastrointestinal diseases, which contains the extract of Ogapi as an active ingredient

The present invention relates to a composition for the prophylaxis or treatment of gastrointestinal diseases selected from the group consisting of gastritis, gastric ulcer and duodenal ulcer containing the extract as an active ingredient.

The stomach is a long-term organ that stores food from the esophagus and breaks it down easily for digestion, and regulates the sending of food to the duodenum in harmony with the secretion of pancreatic enzymes for efficient digestion and absorption. The stomach secretes gastric acid, a strong acid, to digest food when it comes in. The protective layer of the gastric mucosa acts to prevent damage to the gastric mucosa by gastric acid. The gastric mucosal protective layer that protects the stomach is susceptible to damage from various types of factors. Representative attack factors include bacteria such as nonsteroidal anti-inflammatory drugs (NSAID) such as gastric acid, alcohol, aspirin, and helicobacter pylori, microcirculation disorders of hypoxia induced by stress and hypotension. Due to these factors, when the gastric mucosal layer is damaged, inflammation occurs with erosion, redness, bleeding, and swelling. When the gastric mucosa is damaged and penetrates the gastric mucosa and is damaged to the lower mucosa and the muscle layer, gastric ulcer occurs. In addition, due to the above factors, duodenal mucosa is damaged and dug deeper than the mucosal layer on the surface, and damage to the mucosal myocardium occurs.

Therefore, in order to treat gastrointestinal diseases such as gastritis, gastric ulcer and duodenal ulcer caused by the above-mentioned attack factors, inhibition of gastric acid secretion, inhibition of proliferation of Helicobacter pylori bacteria, promotion of mucous secretion, promotion of epithelial cell regeneration, anti-inflammatory, etc. Drugs with efficacy are needed.

At present, the most representative treatments for gastrointestinal diseases include antacids that neutralize excessive gastric secretions, histamine H ₂ receptor antagonists and proton pump inhibitors that inhibit gastric acid secretion, increase gastric lining resistance to digestive fluids, and increase acid resistance. Prostaglandin or gastric mucosa protective agent that can inhibit secretion.

Antacids in the drug are intended for temporary fastness, not for fundamental treatment, and exhibit medicinal effects by lowering the activity of pepsin by raising the pH in the stomach. However, side effects such as constipation, diarrhea, metabolic alkalosis, urolithiasis, and the like have been reported by the administration of an inorganic substance when antacids are used, and recently, acid rebound due to antacids has been a problem.

In addition, cimetidine, the most widely used histamine H ₂ receptor antagonist, blocks histamine receptors in the gastric mucosa and blocks histamine molecules so that histamine molecules do not signal acid secretion. It does not work. Full-scale treatment of gastric ulcers began with the release of cimetidine in 1977, and several derivatives have been developed. Among them, ranitidine, famotidine, roxatidine, and nizatidine have excellent anti-ulcer effects in clinical practice due to shortening of treatment period, but relapse rate after stopping drug administration There is a high disadvantage. In addition, cimetidine exhibits side effects such as decreased libido in men, gynecomastia in men, and neutropenia, and drug interactions, and thus the use of cimetidine has been avoided. Is witnessing a significant decrease.

In addition, proton pump inhibitors include omeprazole and lansoprazol, which are known to exhibit potent acid secretion effects by inhibiting acid secretion in gastric wall cells at a final stage. However, the relapse rate has not been reduced even in ulcer healing patients with these drugs, and side effects such as stool, diarrhea, fever, headache and fatigue have been reported.

As described above, the development of antisecretory agents such as histamine H ₂ receptor antagonists and proton pump inhibitors has been able to cure gastrointestinal diseases requiring surgery until now. However, most of the cured gastrointestinal disorders require long-term maintenance therapy even after recurrence, regeneration, and complete healing, and recurrence or regeneration during maintenance therapy is frequently observed. Therefore, when only histamine H ₂ receptor antagonist and proton pump inhibitor are used for the purpose of inhibiting gastric acid secretion, the most important inhibitory effect of relapse rate cannot be expected in the treatment of gastrointestinal diseases.

In addition, the gastric mucosa protective agent is generally a short-acting, high dose, and has a disadvantage in that it should be taken for a relatively long time, but it is known that the regenerated mucosa recovers similar to normal. Therefore, in recent years, along with gastric acid inhibitors such as antacids, histamine H ₂ receptor antagonists or proton pump inhibitors, sucralfat, bismuth chelates, which protect mucosa, promote tissue repair and increase mucosal blood flow, The same gastric mucoprotectant is used at the same time.

In recent years, antibiotics have been used for the purpose of removing Helicobacter pylori bacteria.

The main symptoms of gastrointestinal diseases such as gastritis, gastric ulcer and duodenal ulcer are abdominal pain, heartburn, bloating, indigestion, burping, nausea, vomiting and bleeding. In order to alleviate the above symptoms, there are cases of taking an antacid or eating a meal quickly, but this is only temporary, and steroidal and nonsteroidal anti-inflammatory drugs are limited in use due to side effects. In particular, nonsteroidal anti-inflammatory drugs, such as aspirin and ibuprofen, have been severely restricted in their use, such as aggravating stomach ulcers or duodenal ulcers. Therefore, there is a need for the development of drugs that can alleviate the symptoms of gastrointestinal diseases and protect the stomach with less side effects even when taken for a long time.

Therefore, researches on the treatment of gastrointestinal diseases using herbal medicines having less side effects than chemical drugs and ensuring stability have been actively conducted. Many herbal medicines, such as mugwort, mortar, turmeric, licorice and lobules, have been studied as a treatment for gastrointestinal diseases using herbal medicines.

On the other hand, Ogapi (五加皮, Acanthopanacis Cortex) is a shrub plant belonging to the family Arboraceae, divided into five leaves. As of Acanthopanax kind in gasiohgapi (Acanthopanax senticosus (RUPR. Et MAX .) HARMS), Acanthopanax wood (Acanthopanax sessiliflorus (RUPR. Et MAX .) SEEM.), King gasiohgapi (Acanthopanax senticosus var. Subinermis KITAGAWA), including 15 paper Korea It exists and has long been classified as a medicinal herb that has no toxicity and side effects in oriental medicine. Ogapi leaves contain chiisanoside, and roots contain not only acanthoside B, D (Acanthoside B, D) but also silrgin and coumarin glycosides. Ogapi also contains water-soluble polysaccharides that enhance immunity. Ogapi is known for its spicy, bitter, warm properties, and acts on the liver, nerves, and nerves to eliminate customs, nourish, and clarify essence. It also protects Oro (Frail disease of the Frail) and Lacquer (Seven Symptoms of Frail Man), which is used for poor leg use, elevates the body and improves energy. It is known to clear the mind, to increase the willpower, to prevent the body from becoming light and old, and to cure the bad blood inside the body cleanly and cleanly. It is known to cure various symptoms such as.

As mentioned above, although various pharmacological effects are known for agar, research on gastric mucosal protection effect is still insignificant.

The inventors of the present invention have been searching for herbal medicines to develop gastrointestinal diseases with fewer side effects, while the extract of Ogapi is effective in preventing and treating gastrointestinal damage caused by chemical factors such as anhydrous ethanol or cysteamine and physical factors such as stress. It was confirmed that the excellent, Helicobacter pylori bacteria inhibitory effect was completed the present invention.

The present invention is to provide a composition for the prevention or treatment of gastrointestinal diseases selected from the group consisting of gastritis, gastric ulcer and duodenal ulcer containing the extract as an active ingredient.

1 is a diagram showing the degree of damage to the gastrointestinal injuries after treating the rat woody skin extract in accordance with the present invention in advance, causing gastrointestinal damage in rats with anhydrous ethanol (preventive effect).

Figure 2 is a diagram showing the damage rate of the gastrointestinal damage site after the treatment of the woody skin extract according to the present invention, after causing the gastrointestinal damage of the rat with anhydrous ethanol (preventive effect).

Figure 3 is a diagram showing the degree of damage to the gastrointestinal damage site with time after administration of the ogapi wood extract according to the invention after causing gastrointestinal damage in rats with anhydrous ethanol (therapeutic effect).

Figure 4 is a diagram showing the damage rate of the gastrointestinal damage site with time after administration of Ogapi wood extract according to the present invention after causing gastrointestinal damage of rats with anhydrous ethanol (therapeutic effect).

Fig. 5 is a pretreatment of the oocyte skin extract (50 mg / kg, 150 mg / kg, 300 mg / kg) according to the present invention before causing the gastrointestinal damage (gastric ulcer) of the rat under stress, and then the stomach of the rat under stress. Figure showing the degree of damage to the gastrointestinal injuries after inducing injury (prevention effect).

Figure 6 is treated with the organo woody extract (50mg / kg, 150mg / kg, 300mg / kg) in accordance with the present invention before causing the gastrointestinal damage (gastric ulcer) of the rat under stress, the stomach of the rat under stress Figure showing the damage rate of the gastrointestinal injuries after inducing injury (prevention effect).

Figure 7 is a diagram showing the degree of damage to the gastrointestinal injuries after administration of the ogapi wood extract (50mg / ㎏) according to the present invention after causing the gastrointestinal damage (gastric ulcer) of the rat under stress (therapeutic effect).

Figure 8 is a diagram showing the degree of damage to the gastrointestinal injuries after administration of the ogapi wood extract (150mg / ㎏) according to the present invention after causing the gastrointestinal damage (gastric ulcer) of the rat under stress (therapeutic effect).

9 is a diagram showing the degree of damage to the gastrointestinal injuries after administration of the ogapi wood extract (300mg / ㎏) according to the present invention after causing the gastrointestinal damage (gastric ulcer) of the rat under stress (therapeutic effect).

Figure 10 shows the damage rate of the gastrointestinal damage site after administration of ogapi wood extract (50mg / kg, 150mg / kg, 300mg / kg) according to the present invention after causing the gastrointestinal damage (gastric ulcer) of the rat under stress Toda (therapeutic effect).

Figure 11 is treated with the organowood extract (50 mg / kg, 150 mg / kg, 300 mg / kg) according to the present invention before inducing gastrointestinal damage (duodenal ulcer) of rats with cysteamine, and then cysteamine This is a diagram showing the degree of damage to the gastrointestinal injuries after amine induced gastrointestinal damage in rats (preventive effect).

FIG. 12 shows that the cystamine extract (50 mg / kg, 150 mg / kg, 300 mg / kg) according to the present invention is treated beforehand to cause gastrointestinal damage (duodenal ulcer) in rats with cysteamine, and then cysteamine. This is a diagram showing the damage rate of the gastrointestinal injuries after amine induced gastrointestinal damage in rats (preventive effect).

Figure 13 is a diagram showing the degree of damage to the gastrointestinal injuries after administration of the ogapi wood extract (50mg / ㎏) according to the present invention after inducing gastrointestinal damage (duodenal ulcer) of rats with cysteamine (therapeutic effect).

Figure 14 is a diagram showing the degree of damage to the gastrointestinal damage after administration of the ogapi wood extract (150mg / ㎏) according to the present invention after inducing gastrointestinal damage (duodenal ulcer) of rats with cysteamine (therapeutic effect).

Figure 15 is a diagram showing the degree of damage to the gastrointestinal damage after administration of the ogapi wood extract (300mg / ㎏) according to the present invention after inducing gastrointestinal damage (duodenal ulcer) of rats with cysteamine (therapeutic effect).

Figure 16 shows the damage to the gastrointestinal injuries after administration of the oocyte skin extract (50 mg / kg, 150 mg / kg, 300 mg / kg) according to the present invention after inducing gastrointestinal damage (duodenal ulcer) in rats with cysteamine. Figure showing rate (therapeutic effect).

The present invention provides a composition for the prevention or treatment of gastrointestinal diseases selected from the group consisting of gastritis, gastric ulcer and duodenal ulcer containing the extract as an active ingredient.

The composition includes a pharmaceutical composition and a food composition.

Hereinafter, the present invention will be described in detail.

Ogapi extract as an active ingredient of the composition according to the present invention is prepared by the following method. First, the oak tree is extracted by adding water, C ₁ to C ₄ alcohol or a mixed solvent of water and C ₁ to C ₄ alcohol (repeated twice). The extract was filtered, concentrated under reduced pressure, and lyophilized to obtain an ogapi extract in powder form. The C ₁ ~ C ₄ alcohol may be selected from 1 to 100% methanol, 1 to 100% ethanol (alcohol), preferably 1 to 100% ethanol (alcohol), more preferably 60 to 95% ethanol ( Spirit).

The Acanthopanax tree leaves, xylem (stems and branches), roots, berries, may be used alone or mixed flowers, etc. gasiohgapi wood (Acanthopanax senticosus (RUPR. Et MAX .) HARMS), King gasiohgapi wood (Acanthopanax senticosus var. subinermis KITAGAWA), Acanthopanax wood (Acanthopanax sessiliflorus (RUPR. et MAX .) SEEM.), Jiri Acanthopanax wood (Acanthopanax chiisanense NAKAI), island Acanthopanax wood (Acanthopanax koreanum NAKAI), hair Acanthopanax wood (Acanthopanax rufinerve NAKAI), Seoul It is preferable to include one or more selected from the group consisting of Acanthopanax seoulense NAKAI, Acanthopanax sieboloians , and Acanthopanax sieboldianum MAKINO.

Ogapi extract of the present invention is excellent in the prevention and treatment of gastrointestinal damage caused by chemical factors such as anhydrous ethanol or cysteamine and physical factors such as stress, and the growth of urease activity and the growth of Helicobacter pylori bacteria By significantly inhibiting, the Helicobacter pylori bacteria inhibitory effect is excellent. Therefore, the extract of Ogapi according to the present invention can be used as a medicine and health functional food useful for the prevention or treatment of gastrointestinal diseases such as gastritis, gastric ulcer and duodenal ulcer.

The composition of the present invention may contain one or more known active ingredients having a prophylactic or therapeutic effect of gastroenteritis, such as gastritis, gastric ulcer and duodenal ulcer, together with the extract of Ogapi.

The composition of the present invention may be prepared by including one or more pharmaceutically acceptable carriers in addition to the above-described active ingredients for administration. Pharmaceutically acceptable carriers may be used in combination with saline, sterile water, Ringer's solution, buffered saline, dextrose solution, maltodextrin solution, glycerol, ethanol and one or more of these components, if necessary, antioxidants, buffers And other conventional additives such as bacteriostatic agents can be added. Diluents, dispersants, surfactants, binders and lubricants may also be added in addition to formulate into injectable formulations, pills, capsules, granules or tablets such as aqueous solutions, suspensions, emulsions and the like. Furthermore, it may be preferably formulated according to each disease or component by a suitable method in the art or using a method disclosed in Remington's Pharmaceutical Science (Recent Edition), Mack Publishing Company, Easton PA.

The composition of the present invention can be administered orally or parenterally (eg, applied intravenously, subcutaneously, intraperitoneally or topically) according to the desired method, and the dosage is based on the weight, age, sex and health of the patient. The range varies depending on the diet, the time of administration, the method of administration, the rate of excretion and the severity of the disease. The daily dose of the Ogapi extract is about 30 to 8,000 mg, preferably administered once to several times a day.

The composition of the present invention may be used alone or in combination with methods using surgery, hormone therapy, drug treatment and biological response modifiers for the prevention or treatment of gastrointestinal diseases such as gastritis, gastric ulcer and duodenal ulcer.

The composition of the present invention may be added to a dietary supplement for the purpose of improving gastrointestinal diseases such as gastritis, gastric ulcer and duodenal ulcer. In the case of using the Ogapi extract of the present invention as a food additive, the Ogapi extract may be added as it is or used with other food or food ingredients, and may be appropriately used according to a conventional method. The mixed amount of the active ingredient may be suitably determined depending on the purpose of use (prevention, health or therapeutic treatment). In general, in the preparation of food or beverage, the extract of the organo of the present invention is added in an amount of 15% by weight or less, preferably 10% by weight or less with respect to the raw material. However, in the case of long-term intake for health and hygiene or health control, the amount may be below the above range, and the active ingredient may be used in an amount above the above range because there is no problem in terms of safety. .

There is no particular limitation on the kind of food. Examples of the food to which the substance can be added include dairy products including meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, ice cream, various soups, drinks, tea, drinks, Alcoholic beverages and vitamin complexes, and includes all of the dietary supplements in the conventional sense.

The health beverage composition of the present invention may contain various flavors or natural carbohydrates, etc. as additional components, as in the general beverage. Natural carbohydrates described above are monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol and erythritol. As the sweetening agent, natural sweetening agents such as tautin and stevia extract, synthetic sweetening agents such as saccharin and aspartame, and the like can be used. The proportion of the natural carbohydrate is generally about 0.01-0.20 g, preferably about 0.04-0.10 g per 100 ml of the composition of the present invention.

In addition to the above, the composition of the present invention includes various nutrients, vitamins, electrolytes, flavors, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, And a carbonation agent used for the carbonated beverage. In addition, the composition of the present invention may contain a pulp for the production of natural fruit juices, fruit juice drinks and vegetable drinks. These components can be used independently or in combination. The proportion of such additives is not critical but is usually selected in the range of 0.01 to 0.20 parts by weight per 100 parts by weight of the composition of the present invention.

Hereinafter, preferred examples are provided to aid in understanding the present invention. However, the following examples are merely provided to more easily understand the present invention, and the contents of the present invention are not limited by the examples.

Example 1  : Preparation of Prickly Pleated Wood Water Extract

Acanthopanax senticosus (RUPR. Et MAX.) HARMS woody parts (stems and branches) were shaded and shredded. 250 g of shredded prickly wood was added to a round bottom flask, 2.5 L of water was added thereto, and then extracted twice at 100 ° C. for 4 hours. The combined extracts were filtered and concentrated to 10 brix under reduced pressure. The concentrated solution was lyophilized with a vacuum freeze dryer to obtain 9.32 g of a hot water extract of prickly woody woody part in powder form.

Example 2  : Preparation of Prickly Pear 70% Ethanol Extract

Shaded and shredded thorny oak wood. 250 g of shredded prickly wood was added to a round bottom flask, 2.5 L of 70% ethanol (alcohol) was added thereto, and then extracted twice at 65 to 80 ° C for 4 hours. The combined extracts were filtered and concentrated to 10 brix under reduced pressure. The concentrated solution was lyophilized with a vacuum freeze dryer to obtain 5.25 g of a 70% ethanol (alcohol) extract of thorny oak wood in powder form.

Example 3  : Preparation of Prickly Pear Wood 95% Ethanol Extract

Shaded and shredded thorny oak wood. 250 g of shredded prickly wood was added to a round bottom flask, and 5 L of 95% ethanol (alcohol) was added thereto, followed by extraction at room temperature for 48 hours. Shake it every 6 hours. The combined extracts were filtered and concentrated to 10 brix under reduced pressure. The concentrated solution was lyophilized with a vacuum freeze dryer to obtain 3.72 g of 95% ethanol (alcohol) extract of thorny oak wood powder in powder form.

Example 4  : Preparation of Prickly Pear Extract

Except for using the Thorn oak tree leaves instead of the Thorn oak tree wood in Example 1, 28.39 g of Thorn oak leaf hydrothermal extract of the powder form was obtained in the same manner as in Example 1.

Example 5  : Preparation of Prickly Pear Extract 70% Ethanol (Alcohol) Extract

23.23 g of a 70% ethanol (alcohol) extract in powder form was obtained in the same manner as in Example 2, except that the thorny oak tree leaf was used instead of the thorny oak tree wood in Example 2.

Example 6  : Preparation of Prickly Pear Extract 95% Ethanol (Alcohol) Extract

Except for using the Thorn oak tree leaves instead of the Thorn oak tree wood in Example 3, in the same manner as in Example 3 to obtain 16.6 g of 95% ethanol (alcohol) extract of Thorn oak leaves in powder form.

Example 7  : Preparation of Sseogapi wood-floor hydrothermal extract

In Example 1, except that the oak bark wood part was used instead of the thorny oak bark wood part, in the same manner as in Example 1, 9.17 g of the sebum oak wood part hot water extract in powder form was obtained.

Example 8  : Preparation of Siogapi wood part 70% ethanol (alcohol) extract

Except for using the island of the oak bark wood in Example 2, instead of the oak bark wood, in the same manner as in Example 2 to obtain 5.51 g of an extract of 70% ethanol (alcohol) extract of the wood oak bark of the powder form.

Example 9  : Preparation of Sseogapi wood part 95% ethanol (alcohol) extract

In Example 3, except that the oak bark wood part was used instead of the oak bark wood part, in the same manner as in Example 3, 3.58 g of the oak bark wood part 95% ethanol (alcohol) extract in powder form was obtained.

Example 10  : Preparation of Whangashiogapi Woodland Hot Water Extract

In Example 1, except that the thorny oak tree wood, instead of the wangoshioga wood, the same as in Example 1 to obtain 9.17g of Whangashiopi wood-heated hydrothermal extract in powder form.

Example 11  : Preparation of 70% Ethanol Extract

In the same manner as in Example 2, except for using the royal wood oak wood wood instead of thorn oak wood wood part in Example 2, 5.51 g of 70% ethanol (alcohol) extract of Wanggao shiga wood in powder form was obtained.

Example 12  : Preparation of Whangashiogapi Wood Part 95% Ethanol Extract

In the same manner as in Example 3, except for using the royal wood oak wood wood part instead of the thorn oak wood wood part in the same manner as in Example 3 to obtain 3.58 g of 95% ethanol (alcohol) extract of powdery Wangga oga wood.

Example 13  : Preparation of Prickly Pear Root Hydrothermal Extract

Except for using the prickly oak tree root instead of the prickly oak tree wood in Example 1, in the same manner as in Example 1 to obtain 33.7g of the prickly oak root hot water extract in powder form.

Example 14  : Preparation of Prickly Pear Extract 70% Ethanol (Alcohol) Extract

Except for using the Thorn oak tree roots instead of the Thorn oak tree wood in Example 2, in the same manner as in Example 2 to obtain 32.75 g of thorny oak root 70% ethanol (alcohol) extract in powder form.

Example 15  : Preparation of Prickly Pear Extract 95% Ethanol (Alcohol) Extract

Except for using the Thorn oak tree roots instead of the Thorn oak tree wood in Example 3, 23.3 g of 95% ethanol (alcohol) extract of the thorn oak root in powder form was obtained in the same manner as in Example 3.

Experimental Example 1  : Preventive and therapeutic effect on gastrointestinal damage caused by anhydrous ethanol of the extract of Ogapi according to the present invention

In order to confirm the prophylactic and therapeutic effects on gastrointestinal damage caused by anhydrous ethanol of the extract of Ogapi according to the present invention, the following experiment was performed.

1. Experimental Animal

Experimental animals received 180-200 g of SD male rats from Daehan Biolink Co., Ltd. under the conditions of animal history (temperature: 20 ± 2 ℃, relative humidity: 40-60%, contrast: 12 hour contrast cycle). Adapted enough for two weeks and raised. Feed was freely ingested solid feed for experimental animals (Korea Biolink Co., Ltd.), water was freely ingested after disinfecting tap water with UV sterilizer. 24 hours before the start of the experiment only watered and fasted. At this time, the experimental animals were treated within a certain time (10:00 AM ~ 12:00 AM) in consideration of the daily variation of enzyme activity. The experimental animals were divided into 3 groups and 5 animals were allocated to the 1 group.

2. Preventive effect on gastrointestinal damage caused by anhydrous ethanol

The test animal prepared in Example 1 was orally administered at 70 hours ethanol extract (150 mg / kg) of the thorny oak wood part prepared in Example 2 at 48 hours, 24 hours and 2 hours before the start of the experiment. After the animal was fasted and watered for 24 hours on the last day of administration, 1 ml / 200 g of anhydrous ethanol solution was orally administered to cause gastrointestinal damage. Then, the stomach was removed by lethal with ether after 2 hours in fasting and saving water. The extracted stomach was soaked in 10 ml of a 2% formalin aqueous solution for 1 hour and fixed. The length of the damaged part (mm) generated by longitudinal incision along the upper curved part of the stomach was measured, and this was regarded as the damage index. Distilled water was used as a negative control group, and cimetidine (150 mg / kg) was used as a positive control group.

Before inducing gastrointestinal damage in rats with anhydrous ethanol in advance, the degree of damage to the gastrointestinal injuries after inducing gastrointestinal damage in rats with anhydrous ethanol is shown in Table 1 and FIG. , The damage rate of the gastrointestinal damage site is shown in Table 1 and FIG.

Statistical processing of the results obtained in the experiment was expressed as mean ± standard deviation. Statistical significance was tested using Duncan's multiple rage test, and determined to be statistically significant at p <0.05.

Table 1 Before inducing gastrointestinal damage in rats with anhydrous ethanol, the woody woody extracts according to the present invention were treated in advance, followed by gastrointestinal damage in rats with anhydrous ethanol.

sample	The extent of damage to the gastrointestinal damage [length (mm)]	Gastrointestinal damage rate (%)
Negative Control (Distilled Water)	61.6 ± 23.0	100
Positive control group (cimetidine)	49.4 ± 29.7	80.2
Example 2 (70% ethanol extract of woody bark wood)	33.7 ± 13.3	54.7

As shown in Table 1, Fig. 1 and Fig. 2, when the organ wood extract of the present invention was previously treated before the rat gastrointestinal damage was induced with anhydrous ethanol, the length of the gastrointestinal injury site caused by the anhydrous ethanol was 33.7. The damage rate of the gastrointestinal lesions was 54.7%, which was less than that of the positive control group (cimetidine). Therefore, it can be seen that the extract of the woody part of the ogapi according to the present invention has an excellent preventive effect against gastrointestinal damage caused by anhydrous ethanol.

3. Therapeutic Effect on Gastrointestinal Damage Caused by Anhydrous Ethanol

After fasting and watering the experimental animals prepared in 1 above 24 hours before the test, oral administration of 1 ml / 200 g of anhydrous ethanol solution to the experimental animals caused gastrointestinal damage. Then, after leaving for 2 hours under fasting and water saving, the 70% ethanol extract (150 mg / kg) of the woody skin part prepared in Example 2 was orally administered at 24 hour intervals. After 24 hours and 48 hours, the stomachs were harvested by ether. The extracted stomach was soaked in 10 ml of a 2% formalin aqueous solution for 1 hour and fixed. The length of the damaged part (mm) generated by cutting the upper part of the stomach was measured, and this was regarded as the damage index. Distilled water was used as a negative control group, and cimetidine (150 mg / kg) was used as a positive control group.

After inducing the gastrointestinal damage of rats with anhydrous ethanol, the degree of damage to the gastrointestinal lesions with time after administration of the extract of the woody oocyte extract according to the present invention is shown in Table 2 and FIG. 4 is shown.

TABLE 2 The extent of damage to the gastrointestinal lesions caused by anhydrous ethanol over time after administration of the extract of Ogapi woody parts of the present invention

sample	The extent of damage to the gastrointestinal damage [length (mm)]
	0 hr	24 hr	48 hr
Negative Control (Distilled Water)	56.7 ± 14.7	41.2 ± 14.8	29.4 ± 9.6
Positive control group (cimetidine)	-	18.5 ± 0.7	8.0 ± 0.0
Example 2 (70% ethanol extract of woody bark wood)	-	13.8 ± 7.1	6.7 ± 4.2

TABLE 3 Damage rate of gastrointestinal damage site caused by anhydrous ethanol with time after administration of Ogapi woody part extract of the present invention

sample	Gastrointestinal damage rate (%)
	0 hr	24 hr	48 hr
Negative Control (Distilled Water)	100	72.7	51.9
Positive control group (cimetidine)	100	32.6	14.1
Example 2 (70% ethanol extract of woody bark wood)	100	24.3	11.8

As shown in Tables 2 to 3 and Figures 3 to 4, the length of the gastrointestinal injuries of rats induced by anhydrous ethanol 24 hours after the administration of the extract of the woody oocyte extract according to the present invention is 13.8 ± 7.1 mm, and the damage of the gastrointestinal injuries The rate was 24.3%, and the length of gastrointestinal injuries caused by anhydrous ethanol after 48 hours was 6.7 ± 4.2mm, and the incidence of gastrointestinal injuries was 11.8%, compared to the positive control group (cimetidine). The rate was low. In addition, the longer the time after administration of the extract of the oak skin wood extract of the present invention showed less degree of gastrointestinal damage and damage rate. Therefore, it can be seen that the extract of the woody oocytes according to the present invention has an excellent therapeutic effect on gastrointestinal damage caused by anhydrous ethanol.

Experimental Example 2  : Preventive and therapeutic effects on gastrointestinal damage (gastrointestinal ulcer) induced by stress of Ogapi extract according to the present invention

In order to confirm the prophylactic and therapeutic effects on gastrointestinal damage (gastric ulcer) caused by stress of the extract of Ogapi according to the present invention, the following experiment was performed.

1. Experimental Animal

The experimental animals were fed SD male rats weighing 200-220g from Daehan Biolink Co., Ltd. under constant conditions of animal history [temperature: 23 ± 3 ℃, relative humidity: 55 ± 15%, lighting time: 12 hours (8 AM) C. ~ 8 pm), roughness: 150 ~ 300Lux] was enough for 2 weeks to breed. Feed was freely ingested solid feed for experimental animals (Korea Biolink Co., Ltd.), water was freely ingested after disinfecting tap water with UV sterilizer. 24 hours before the start of the experiment only watered and fasted. At this time, the experimental animals were treated within a certain time (10:00 AM ~ 12:00 AM) in consideration of the daily variation of enzyme activity. Experimental animals were divided into five groups [① negative control group, ② experimental group 1: thorny oak woody part 70% ethanol (alcohol) extract (low concentration), ③ experimental group 2: thorny oak woody part 70% ethanol (alcohol) extract (medium), ④ experimental group 3: thorny oak skin 70% ethanol (alcohol) extract (high concentration), ⑤ positive control group of wood, 24 rats were allocated only to the negative control group, and 18 animals were allocated to the remaining experimental groups 1, 2, 3 and the positive control group. It was.

2. Preventive effect on stress-induced gastrointestinal damage (gastric ulcer)

The test animal prepared in Example 1 was orally administered 70% ethanol extract [50 mg / kg, 150 mg / kg, 300 mg / kg] of the woody skin part prepared in Example 2 from 7 days before the start of the experiment. On the last day of administration, the animals were fasted and watered for 24 hours, and then corrected in a restraint device, and the animals were submerged in a water bath at 22-24 ° C until the xiphoid process was stressed by the water restraint method. Gastric ulcers). After 20 hours of immersion, the animals were sacrificed and the stomachs were extracted. The extracted stomach was soaked in 10 ml of a 1% formalin aqueous solution for 1 hour and fixed. The incision was made along the upper Taiwan curve, lightly rubbed the contents of the stomach with kimwipes, and the stomach was spread on a cork board. The length of the gastrointestinal injuries was measured (mm) and this was used as the index of injury (in case of bleeding hemorrhage, 5 were counted as 1 mm, and it was given from 0 to 6 points according to the degree of injury. 0 point, 1 point for 1 ~ 5mm damage, 1 point for 6 ~ 10mm damage, 2 points for damage of 11 ~ 15mm, 3 points for damage of 11 ~ 15mm, 4 points for damage of 16 ~ 20mm , 5 points for 21-25mm damage and 6 points for damage and puncture of 26mm or more). Distilled water was used as a negative control group, and styrene tablet (Dong-A Pharm, 150 mg / kg) was used as a positive control group.

Before the rat gastrointestinal damage (gastric ulcer) is induced by stress, the organo woody extract according to the present invention is treated in advance, and then the degree of damage (damage score and damage rate) of the gastrointestinal damage site after stress is induced by the rat gastrointestinal damage. Table 4 and Figures 5-6.

Table 4 Before the rat gastrointestinal damage (gastric ulcer) is induced by stress, the woody skin extract according to the present invention is treated in advance, and then the gastrointestinal damage of the rat is induced by stress (injury score and damage rate): Prevention effect

sample		The degree of damage to the stressed gastrointestinal injury (gastric ulcer)
		Damage score	% Damage
Negative Control (Distilled Water)		3.00	100
Example 2 (70% ethanol extract of woody bark wood)	50 mg / kg	2.40	80
	150mg / kg	2.40	80
	300 mg / kg	1.83	61.1
Positive control group (styrene tablets, 150 mg / kg)		2.00	66.7

As shown in Table 4 and Figures 5 to 6, when the organo woody extract of the present invention was previously treated before causing the gastrointestinal damage (gastric ulcer) of the rat under stress, the damage rate of the gastrointestinal damage site caused by stress was 61.1-80.0% showed less gastrointestinal damage than the positive control group (styrene tablets). Therefore, it can be seen that the extract of the woody pulp part according to the present invention has an excellent preventive effect against gastrointestinal damage (gastric ulcer) caused by stress.

3. Therapeutic effect on stress-induced gastrointestinal damage (gastric ulcer)

After fastening and watering the experimental animals prepared in 1 above 24 hours before the start of the experiment and corrected in the restraint device, the animals were submerged in a water tank at 22-24 ° C to the dendritic spine to give stress to the gastrointestinal restraint method (gastric ulcer) ). Next, the experimental animals were orally administered 70% ethanol extract [50 mg / kg, 150 mg / kg, 300 mg / kg] in the woody pulp part prepared in Example 2 for 1 day and 3 days. The stomach was then harvested at the expense of the experimental animals. The extracted stomach was soaked in 10 ml of a 1% formalin aqueous solution for 1 hour and fixed. The incision was made along the upper Taiwan valley, and the contents of the stomach were lightly rubbed with Kimwipes and the stomach was spread over the corkboard. The length of the gastrointestinal injuries was measured (mm) and this was used as the index of injury (in case of bleeding hemorrhage, 5 was calculated as 1 mm, depending on the degree of injury, from 0 to 6 points. 0 point, 1 point for 1 ~ 5mm damage, 1 point for 6 ~ 10mm damage, 2 points for damage of 11 ~ 15mm, 3 points for damage of 11 ~ 15mm, 4 points for damage of 16 ~ 20mm , 5 points for 21-25mm damage and 6 points for damage and puncture of 26mm or more). Distilled water was used as a negative control group, and styrene tablet (Dong-A Pharm, 150 mg / kg) was used as a positive control group.

After the stress induced gastrointestinal damage (gastric ulcer) of rats, the degree of damage (damage score and damage rate) of the gastrointestinal damage site after administration of the woody oocyte extract according to the present invention is shown in Tables 5 to 6 and FIGS. 7 to 10.

Table 5 Damage score of gastrointestinal damage site after administration of Ogapi wood extract according to the present invention after stress induced gastrointestinal damage (gastric ulcer) of rats: therapeutic effect

sample		Damage score of stressed gastrointestinal injuries (gastric ulcer)
		1 day	3 days
Negative Control (Distilled Water)		2.60	1.80
Example 2 (70% ethanol extract of woody bark wood)	50 mg / kg	1.50	1.00
	150mg / kg	1.33	1.00
	300 mg / kg	0.83	0.83
Positive control group (styrene tablets, 150 mg / kg)		1.20	1.00

Table 6 Damage rate of gastrointestinal injuries after administration of Ogapi wood extract according to the present invention after stress induced gastrointestinal damage (gastric ulcer) in rats: therapeutic effect

sample		% Damage to stress gastrointestinal injuries (gastric ulcer)
		1 day	3 days
Negative Control (Distilled Water)		88.6	60.0
Example 2 (70% ethanol extract of woody bark wood)	50 mg / kg	50.0	33.3
	150mg / kg	44.4	33.3
	300 mg / kg	27.8	27.8
Positive control group (styrene tablets, 150 mg / kg)		40.0	33.3

As shown in Tables 5-6 and Figures 7-10, the damage rate of the gastrointestinal injuries was 27.8 ~ on the first day when the Ogapi wood extract was administered according to the present invention after causing the gastrointestinal injury (gastric ulcer) of the rat under stress. At 50.0% and day 3, 27.8 ~ 33.3% showed less gastrointestinal damage than the positive control group (styrene tablets). Therefore, it can be seen that the ogapi wood extract according to the present invention has an excellent therapeutic effect against stress-induced gastrointestinal damage (gastric ulcer).

Experimental Example 3  : Preventive and therapeutic effects on gastrointestinal damage (duodenal ulcer) induced by cysteamine of Ogapi extract according to the present invention

In order to confirm the preventive and therapeutic effects on gastrointestinal damage (duodenal ulcer) caused by cysteamine of the extract of Ogapi according to the present invention, the following experiment was performed.

1. Preventive effect on gastrointestinal damage (duodenal ulcer) caused by cysteamine

Experimental animals and sample dosage conditions were the same as in Example 2. A cysteamine HCl (10%) solution was prepared by adding 5 g of cysteamine HCl to 50 ml of distilled water. Experimental animals were orally administered 70% ethanol extract [50 mg / kg, 150 mg / kg, 300 mg / kg] prepared in Example 2 from 7 days before the start of the experiment. After the animals were fasted and watered for 24 hours on the last day of administration, gastrointestinal damage (duodenal ulcer) was induced by subcutaneous injection of 300 mg / kg of cysteamine HCl (10%). Twenty-four hours after the initial cysteamine administration, the animals were sacrificed and the duodenum was extracted. The extracted duodenum was soaked in 10 ml of 1% formalin aqueous solution for 1 hour and fixed. A longitudinal incision was made along the mesentery of the duodenum and the degree of ulceration was scored (in case of bleeding hemorrhage was counted as 1 mm and given from 0 to 6 points according to the degree of injury. 1 point for damage of 1 to 5 mm, 2 points for damage of 6 to 10 mm, 3 points for damage of 11 to 15 mm, 4 points for damage of 16 to 20 mm 5 points for damage of ˜25 mm and 6 points for damage and puncture of 26 mm or more). Distilled water was used as a negative control group, and styrene tablet (Dong-A Pharm, 150 mg / kg) was used as a positive control group.

Before inducing gastrointestinal damage (duodenal ulcer) of rats with cysteamine, the organowood extract according to the present invention was treated in advance, and then gastrointestinal damage of rats was induced with cysteamine (damage score). And damage rate) are shown in Table 7 and FIGS. 11 to 12.

TABLE 7 Before inducing gastrointestinal damage (duodenal ulcer) in rats with cysteamine, the organowood extract of the present invention was treated in advance, and then gastrointestinal damage was induced in rats with cysteamine (damage score). And damage rate): Preventive effect

sample		Extent of damage to the gastrointestinal damage (duodenal ulcer)
		Damage score	% Damage
Negative Control (Distilled Water)		1.17	100
Example 2 (70% ethanol extract of woody bark wood)	50 mg / kg	0.83	71.4
	150mg / kg	0.67	57.1
	300 mg / kg	0.50	42.9
Positive control group (styrene tablets, 150 mg / kg)		0.67	57.1

As shown in Table 7 and Figures 11-12, gastrointestinal damage caused by cysteamine when pretreated with the organodermal extract of the present invention prior to causing gastrointestinal damage (duodenal ulcer) in rats with cysteamine The damage rate of the site was 42.9 ~ 71.4%, which showed less gastrointestinal damage than the positive control group (styrene tablet). Therefore, it can be seen that the extract of the woody oocyte skin according to the present invention has an excellent preventive effect against gastrointestinal damage (duodenal ulcer) caused by cysteamine.

2. Therapeutic effect on gastrointestinal damage (duodenal ulcer) caused by cysteamine

After fasting and saving water from 24 hours before the start of the experiment, gastrointestinal damage (duodenal ulcer) was induced by subcutaneous injection of 300 mg / kg of cysteamine HCl (10%). Then, the experimental animals were orally administered 70% ethanol extract [50 mg / kg, 150 mg / kg, 300 mg / kg] in the woody pulp part prepared in Example 2 for 1 day and 3 days. The duodenum was then extracted at the expense of the experimental animals. The extracted duodenum was soaked in 10 ml of 1% formalin aqueous solution for 1 hour and fixed. A longitudinal incision was made along the mesentery of the duodenum and the degree of ulceration was scored (in case of bleeding hemorrhage was counted as 1 mm and given from 0 to 6 points according to the degree of injury. 1 point for damage of 1 to 5 mm, 2 points for damage of 6 to 10 mm, 3 points for damage of 11 to 15 mm, 4 points for damage of 16 to 20 mm 5 points for damage of ˜25 mm and 6 points for damage and puncture of 26 mm or more). Distilled water was used as a negative control group, and styrene tablet (Dong-A Pharm, 150 mg / kg) was used as a positive control group.

After inducing rat gastrointestinal damage (duodenal ulcer) with cysteamine, the degree of damage (damage score and damage rate) of the gastrointestinal damage site after administration of the woody oocyte extract according to the present invention is shown in Tables 8-9 and FIGS. 13-16. Indicated.

Table 8 Damage score of gastrointestinal injuries after administration of Ogapi wood extract according to the present invention after inducing gastrointestinal damage (duodenal ulcer) in rats with cysteamine: therapeutic effect

sample		Injury score of gastrointestinal injuries (duodenal ulcer)
		1 day	3 days
Negative Control (Distilled Water)		0.67	0.67
Example 2 (70% ethanol extract of woody bark wood)	50 mg / kg	0.50	0.33
	150mg / kg	0.33	0.17
	300 mg / kg	0.50	0.17
Positive control group (styrene tablets, 150 mg / kg)		0.67	0.50

Table 9 Injury rate of gastrointestinal lesions after administration of Ogapi wood extract according to the present invention after inducing gastrointestinal damage (duodenal ulcer) in rats with cysteamine: therapeutic effect

sample		% Damage to gastrointestinal damage (duodenal ulcer)
		1 day	3 days
Negative Control (Distilled Water)		57.1	57.1
Example 2 (70% ethanol extract of woody bark wood)	50 mg / kg	42.9	29.6
	150mg / kg	29.6	14.3
	300 mg / kg	42.9	14.3
Positive control group (styrene tablets, 150 mg / kg)		57.0	42.9

As shown in Tables 8-9 and FIGS. 13-16, the gastrointestinal damage of rats with cysteamine induced duodenal ulcer, and then the rate of damage of the gastrointestinal lesions was 1 day. There was less gastrointestinal damage in 29.6 ~ 42.9% and 14.3 ~ 29.6% at 3 days. Therefore, it can be seen that the extract of the woody pulp part according to the present invention has an excellent therapeutic effect on gastrointestinal damage (duodenal ulcer) caused by cysteamine.

Experimental Example 4  : Helicobacter pylori bacterium inhibitory effect of Ogapi extract according to the present invention

In order to confirm the inhibitory effect of Helicobacter pylori bacterium of the extract of Ogapi according to the present invention, the following experiment was performed.

1. Inhibitory effect of urease activity

Cell culture tube for urease activity assay [Bacterial yeast extract 0.1g, potassium phosphate (KH ₂ PO ₄ ) 9.1g, potassium diphosphate (K ₂ HPO ₄ ) 9.5g, urea 20g, phenol red 0.01g 7 ml of distilled water, pH 6.8] was added, and 5 × 10 ⁴ of Helicobacter pyrroli bacteria [KE26695 (= ATCC700392)] were dispensed into respective cell culture tubes. After dispensing, the concentrations of the extracts of the woody pulp extract prepared in Examples 1 and 2 were treated to be 250 μg / ml, and then the optimum conditions in the incubator (culture temperature 37 ° C., 10% CO ₂ , 5% O ₂ , 100%). Humidity). After incubation, the absorbance at 550 nm was measured at 24 hours, 48 hours, 72 hours using a spectrophotometer. The absorbance of the culture tube cultured only with Helicobacter pyrroli as 100% was evaluated as a relative value compared with the absorbance of each test sample.

Urea dehydrogenase activity according to the incubation time of the extract of the oak skin wood extract according to the present invention is shown in Table 10.

Table 10 Urea dehydrogenase activity according to incubation time of Ogapi woody extracts according to the present invention

sample	Urease activity (%)
	24 hours	48 hours	72 hours
Control group (Helicobacter pyrroli)	100	100	100
Example 1 (Kashigapi wood part hot water extract)	90	22	31
Example 2 (70% ethanol extract of woody bark wood)	68	16	33

As shown in Table 10, the extract of the shredded oak skin according to the present invention effectively inhibited urease activity, in particular, the most effective inhibitory effect of the urease activity of the 70% ethanol extract of shredded oak skin.

2. Helicobacter pylori growth inhibitory effect: paper disk (paper disk) method 1

Helicobacter pyrroli bacteria [KE26695 (= ATCC700392)] (adjusted to 10 ⁸ -10 ⁹ cfu / ml) were plated with Wang's medium (28 g of Brussela culture, 1.2% agar, 10% horse serum, 1 L of distilled water) with a triangle glass rod. . A paper disk (adjusting the sample concentration to 1500 µg / ml) was placed on Wang's medium stained with Helicobacter pyrroli. Plates were incubated at 37 ° C. for 3 days under microaerobic conditions (10% CO ₂ , 5% O ₂ , 100% humidity). Using the distilled water as a control, the clear zone size (mm) of Helicobacter pyrroli bacteria of each sample was measured and observed. Growth hypotonic ring size is the average of the results obtained by repeating three or more times for each example.

The growth-lowering ring size of the Helicobacter pylori bacterium [KE26695 (= ATCC700392)] of the Ogapi extract according to the present invention is shown in Table 11.

Table 11 Growth Hypoxic Ring Size of Helicobacter pylori [KE26695 (= ATCC700392)] of Ogapi Extract According to the Present Invention

sample	Growth low ring size (mm)
	KE26695 (= ATCC700392)
Control group (distilled water)	0
Example 1	14
Example 2	15

As shown in Table 11, Ogapi extract according to the present invention was confirmed to significantly inhibit the growth of Helicobacter pylori bacteria [KE26695 (= ATCC700392)].

3. Helicobacter pylori growth inhibitory effect: paper disk method 2

Helicobacter pylori bacteria (KCTC12083, KCTC5335) were incubated at 37 ° C. for 3 days in Brucella agar medium (containing 10% sheep blood), and then Helicobacter pylori bacteria (controlled at 10 ⁸ -10 ⁹ cfu / ml) The plate was plated in medium (including 10% sheep blood). A paper disk was placed on Brucellar agar medium stained with Helicobacter pylori, diluted to a concentration of 1 mg / ml, and then absorbed by 10 µl into the paper disk. Plates were incubated at 37 ° C. for 3 days under microaerobic conditions (10% CO ₂ , 5% O ₂ , 100% humidity). Using the distilled water as a control, the growth-lowering ring size (mm) of Helicobacter pylori bacteria of each sample was measured and observed. Growth hypotonic ring size is the average of the results obtained by repeating three or more times for each example.

The growth-lowering ring size of the Helicobacter pylori bacteria (KCTC12083, KCTC5335) of the extract of Ogapi according to the present invention is shown in Table 12.

Table 12 Growth Inhibitory Ring Size of Helicobacter Pylori (KCTC12083, KCTC5335) of Ogapi Extract According to the Present Invention

sample	Growth low ring size (mm)
	KCTC12083	KCTC5335
Control group (distilled water)	0	0
Example 1	13	13
Example 2	22	22
Example 4	14	13
Example 5	18	20
Example 6	18	19
Example 7	12	12
Example 8	16	20
Example 9	17	17
Example 10	15	15
Example 11	23	23
Example 12	23	18
Example 13	14	14
Example 14	19	18

As shown in Table 12, Ogapi extract according to the present invention was confirmed to significantly inhibit the growth of Helicobacter pylori bacteria (KCTC12083, KCTC5335).

Examples of preparations for the compositions of the present invention are illustrated below.

Formulation Example 1  : Preparation of Pharmaceutical Formulations

1. Preparation of powder

2g Ogapi Extract

1g lactose

The above ingredients were mixed and filled in airtight cloth to prepare a powder.

2. Preparation of Tablets

Ogapi Extract 100mg

Corn Starch 100mg

Lactose 100mg

2 mg magnesium stearate

After mixing the above components, tablets were prepared by tableting according to a conventional method for producing tablets.

3. Preparation of Capsule

Ogapi Extract 100mg

Corn Starch 100mg

Lactose 100mg

2 mg magnesium stearate

After mixing the above components, the capsule was prepared by filling in gelatin capsules according to the conventional method for producing a capsule.

Formulation Example 2  : Manufacture of food

Foods containing the extract of the present invention was prepared as follows.

1. Preparation of Cooking Seasonings

Ogapi extract was prepared in 20 ~ 95% by weight health promotion cooking seasoning.

2. Preparation of Tomato Ketchup and Sauce

0.2 ~ 1.0% by weight of the extract Ogapi was added to tomato ketchup or sauce to prepare a tomato ketchup or sauce for health promotion.

3. Manufacturing of Flour Foods

0.5 ~ 5.0 wt% of Ogapi extract was added to the flour, and bread, cake, cookies, crackers and noodles were prepared using the mixture to prepare foods for health promotion.

4. Preparation of soups and gravy

Ogapi extract 0.1 ~ 5.0% by weight was added to the soup and gravy to prepare a health-promoting meat products, noodles soup and gravy.

5. Preparation of Ground Beef

10 wt% of the Ogapi extract was added to the ground beef to prepare a ground beef for health promotion.

6. Manufacture of Dairy Products

5-10% by weight of Ogapi extract was added to milk, and various milk products such as butter and ice cream were prepared using the milk.

Formulation Example 3  : Preparation of Beverages

1. Preparation of Carbonated Drinks

Syrup was made by mixing 10-15% of Ogapi extract, 5-10% of sugar, 0.05-0.3% citric acid, 0.005-0.02% caramel, 0.1-1% of vitamin C, and 75-80% purified water. . The syrup was sterilized at 85 to 98 ° C. for 20 to 180 seconds, mixed with cooling water at a ratio of 1: 4, and 0.5 to 0.82% of carbon dioxide was injected to prepare a carbonated beverage containing the Ogapi extract of the present invention.

2. Manufacture of health drinks

Ogapi extract (2.5% solids, 97.16%), jujube extract (65 brix, 2.67%), overweight extract (solids 70%, 0.12%), vitamin C (0.02%), calcium pantonthenate (0.02%), licorice extract (65% solids, 0.01% solids) was homogeneously blended and instant sterilized and then packaged in small packaging containers such as glass bottles and plastic bottles to prepare healthy drinks.

3. Preparation of Vegetable Juice

5 g of the extract of Ogapi was added to 1,000 ml of tomato or carrot juice to prepare vegetable juice for health promotion.

4. Preparation of Fruit Juice

1 g of the Ogapi extract was added to 1,000 ml of apple or grape juice to prepare fruit juice for health promotion.

Ogapi extract of the present invention is excellent in the prevention and treatment of gastrointestinal damage caused by chemical factors such as anhydrous ethanol or cysteamine and physical factors such as stress, and the growth of urease activity and the growth of Helicobacter pylori bacteria By significantly inhibiting, the Helicobacter pylori bacteria inhibitory effect is excellent. Therefore, the extract of Ogapi according to the present invention can be usefully used for the prevention or treatment of gastrointestinal diseases such as gastritis, gastric ulcer and duodenal ulcer.

Claims (13)

1. Acanthopanax C ₁ ~ C ₄ alcohol extract of gastritis comprising as an active ingredient, gastric ulcer and compositions for the prevention and treatment of gastrointestinal disease is selected from the group consisting of duodenal ulcer.
2. According to claim 1, wherein the C ₁ ~ C ₄ alcohol is a composition for the prevention or treatment of gastrointestinal diseases, characterized in that 1 to 100% ethanol (alcohol).
3. The method for preventing or treating gastrointestinal diseases according to claim 1, wherein the organ clove is mixed with one or more selected from the group consisting of leaves, wood parts (stems and branches), roots, fruits and flowers.
4. The method of claim 1, wherein the Acanthopanax is gasiohgapi wood (Acanthopanax senticosus (RUPR. Et MAX .) HARMS), king gasiohgapi wood (Acanthopanax senticosus var. Subinermis KITAGAWA), Acanthopanax wood (Acanthopanax sessiliflorus (RUPR. Et MAX .) SEEM. ), Jiri Acanthopanax wood (Acanthopanax chiisanense NAKAI), island Acanthopanax wood (Acanthopanax koreanum NAKAI), hair Acanthopanax wood (Acanthopanax rufinerve NAKAI), Seoul Acanthopanax wood (Acanthopanax seoulense NAKAI), per Acanthopanax (Acanthopanax sieboloians) and Keo wood (Acanthopanax sieboldianum MAKINO) composition for the prevention or treatment of gastrointestinal diseases, characterized in that it comprises one or more selected from the group consisting of.
5. The composition for preventing or treating gastrointestinal diseases according to any one of claims 1 to 4, wherein the gastrointestinal diseases are gastrointestinal damages caused by chemical factors such as anhydrous ethanol or cysteamine.
6. The composition for preventing or treating gastrointestinal diseases according to any one of claims 1 to 4, wherein the gastrointestinal disease is gastrointestinal damage caused by physical factors such as stress.
7. The method for preventing or treating gastrointestinal diseases according to any one of claims 1 to 4, wherein the gastrointestinal disease is gastrointestinal damage caused by the bacterium Helicobacter pylori.
8. A composition for the prevention or treatment of gastrointestinal diseases, selected from the group consisting of gastritis, gastric ulcer and duodenal ulcer containing hot water extract of Ogapi as an active ingredient.
9. The composition for preventing or treating gastrointestinal diseases according to claim 8, wherein the organ clover is mixed with one or more selected from the group consisting of leaves, wood parts (stems and branches), roots, fruits and flowers.
10. The method of claim 8, wherein the Agathopanax senticosus (RUPR. Et MAX.) HARMS, Acanthopanax senticosus var. Subinermis KITAGAWA, Acanthopanax sessiliflorus (RUPR. Et MAX.) SEEM. ), Jiri Acanthopanax wood (Acanthopanax chiisanense NAKAI), island Acanthopanax wood (Acanthopanax koreanum NAKAI), hair Acanthopanax wood (Acanthopanax rufinerve NAKAI), Seoul Acanthopanax wood (Acanthopanax seoulense NAKAI), per Acanthopanax (Acanthopanax sieboloians) and Keo wood (Acanthopanax sieboldianum MAKINO) composition for the prevention or treatment of gastrointestinal diseases, characterized in that it comprises one or more selected from the group consisting of.
11. The method of any one of claims 8 to 10, wherein the gastrointestinal disease is gastrointestinal damage caused by chemical factors such as anhydrous ethanol or cysteamine.
12. 11. The composition for preventing or treating gastrointestinal diseases according to any one of claims 8 to 10, wherein the gastrointestinal disease is gastrointestinal damage caused by physical factors such as stress.
13. The method for preventing or treating gastrointestinal diseases according to any one of claims 8 to 10, wherein the gastrointestinal disease is gastrointestinal damage caused by the bacterium Helicobacter pylori.

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