WO2010032945A3 - Immobilization of biomolecules using cholesterol - Google Patents

Immobilization of biomolecules using cholesterol Download PDF

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Publication number
WO2010032945A3
WO2010032945A3 PCT/KR2009/005240 KR2009005240W WO2010032945A3 WO 2010032945 A3 WO2010032945 A3 WO 2010032945A3 KR 2009005240 W KR2009005240 W KR 2009005240W WO 2010032945 A3 WO2010032945 A3 WO 2010032945A3
Authority
WO
WIPO (PCT)
Prior art keywords
biomolecules
immobilization
cholesterol
solid surface
cholesteryl group
Prior art date
Application number
PCT/KR2009/005240
Other languages
French (fr)
Korean (ko)
Other versions
WO2010032945A2 (en
Inventor
이중환
김윤동
채영찬
류성호
강현구
권미진
박성영
장승기
김종인
임종훈
김일신
이승진
최서영
석민경
이혜정
장은정
Original Assignee
포항공과대학교 산학협력단
주식회사 프스코
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 포항공과대학교 산학협력단, 주식회사 프스코 filed Critical 포항공과대학교 산학협력단
Publication of WO2010032945A2 publication Critical patent/WO2010032945A2/en
Publication of WO2010032945A3 publication Critical patent/WO2010032945A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Endocrinology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Steroid Compounds (AREA)

Abstract

Provided are a method for immobilizing biomolecules on a solid surface through a cholesteryl group, a device for detecting biomolecules containing both a solid surface and a capture probe immobilized on the solid surface through a cholesteryl group, and a method for detecting biomolecules using the same.
PCT/KR2009/005240 2008-09-16 2009-09-15 Immobilization of biomolecules using cholesterol WO2010032945A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US9739108P 2008-09-16 2008-09-16
US61/097,391 2008-09-16

Publications (2)

Publication Number Publication Date
WO2010032945A2 WO2010032945A2 (en) 2010-03-25
WO2010032945A3 true WO2010032945A3 (en) 2010-06-24

Family

ID=42039993

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2009/005240 WO2010032945A2 (en) 2008-09-16 2009-09-15 Immobilization of biomolecules using cholesterol

Country Status (1)

Country Link
WO (1) WO2010032945A2 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5856202A (en) * 1994-11-15 1999-01-05 Asahi Kasei Kogyo Kabushiki Kaisha Method for determining antistreptolysin O antibody
US20070275047A1 (en) * 2004-02-26 2007-11-29 Indriati Pfeiffer Oligonucleotides Related to Lipid Membrane Attachments
US20080241942A1 (en) * 2007-03-26 2008-10-02 Xiaoyang Zhu Air-stable supported lipid bilayer membranes

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5856202A (en) * 1994-11-15 1999-01-05 Asahi Kasei Kogyo Kabushiki Kaisha Method for determining antistreptolysin O antibody
US20070275047A1 (en) * 2004-02-26 2007-11-29 Indriati Pfeiffer Oligonucleotides Related to Lipid Membrane Attachments
US20080241942A1 (en) * 2007-03-26 2008-10-02 Xiaoyang Zhu Air-stable supported lipid bilayer membranes

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
A. DEEPU ET AL.: "Simple surface modification techniques for immobilization of biomolecules on SU-8.", J. MATER. SCI.: MATER. MED., vol. 20, 17 June 2008 (2008-06-17), pages S25 - S28 *
S. BALAMURUGAN ET AL.: "Surface immobilization methods for aptamer diagnostic applications.", ANAL. BIOANAL. CHEM., vol. 390, no. 4, 23 September 2007 (2007-09-23), pages 1009 - 1021 *

Also Published As

Publication number Publication date
WO2010032945A2 (en) 2010-03-25

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