WO2010025404A1 - High-throughput and non-invasive method to vitrify porcine embryos - Google Patents

High-throughput and non-invasive method to vitrify porcine embryos Download PDF

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Publication number
WO2010025404A1
WO2010025404A1 PCT/US2009/055424 US2009055424W WO2010025404A1 WO 2010025404 A1 WO2010025404 A1 WO 2010025404A1 US 2009055424 W US2009055424 W US 2009055424W WO 2010025404 A1 WO2010025404 A1 WO 2010025404A1
Authority
WO
WIPO (PCT)
Prior art keywords
embryos
embryo
lipid
porcine
vitro
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2009/055424
Other languages
English (en)
French (fr)
Inventor
Randall S. Prather
Rongfeng Li
Clifton N. Murphy
Lee Spate
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Missouri Columbia
University of Missouri St Louis
Original Assignee
University of Missouri Columbia
University of Missouri St Louis
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Missouri Columbia, University of Missouri St Louis filed Critical University of Missouri Columbia
Priority to CN2009801428076A priority Critical patent/CN102197130A/zh
Priority to JP2011525249A priority patent/JP2012501187A/ja
Priority to CA2741754A priority patent/CA2741754A1/en
Priority to US13/061,222 priority patent/US8852078B2/en
Priority to AU2009285530A priority patent/AU2009285530A1/en
Priority to BRPI0918262A priority patent/BRPI0918262A2/pt
Priority to EP09810683.4A priority patent/EP2331677A4/en
Publication of WO2010025404A1 publication Critical patent/WO2010025404A1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A01N1/12—Chemical aspects of preservation
    • A01N1/122—Preservation or perfusion media
    • A01N1/125—Freeze protecting agents, e.g. cryoprotectants or osmolarity regulators

Definitions

  • lipid-separated porcine embryos may be cryopreserved after further embryo development to the blastocyst stage and subjecting such to vitrification.
  • the vitrified embryos may be warmed, have their zonae pellucidae removed, and transferred into a recipient (such as a surrogate pig).
  • FIGs. 2(a)-(f) are the photos of IVF-derived embryos after high osmolality treatment (at 400 mOsm with NaCl).
  • FIG. 2(a) shows the embryos cultured for several hours after high osmolality treatment and centrification.
  • FIGs. 2(b) and 2(c) show the embryos at blastocyst stage.
  • FIG. 2(d) shows the embryos after vitrification and warming.
  • FIG. 2(e) shows the embryos after removal of their zona pellucida.
  • FIG. 2(f) shows the re-expanded embryos after in vitro culture in BRL cell conditioned medium.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
PCT/US2009/055424 2008-08-29 2009-08-28 High-throughput and non-invasive method to vitrify porcine embryos Ceased WO2010025404A1 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
CN2009801428076A CN102197130A (zh) 2008-08-29 2009-08-28 高通量和非侵袭性玻璃化猪胚胎的方法
JP2011525249A JP2012501187A (ja) 2008-08-29 2009-08-28 ブタ胚をガラス化する高スループットおよび非侵襲的方法
CA2741754A CA2741754A1 (en) 2008-08-29 2009-08-28 High-throughput and non-invasive method to vitrify porcine embryos
US13/061,222 US8852078B2 (en) 2008-08-29 2009-08-28 High-throughput and non-invasive method to vitrify porcine embryos
AU2009285530A AU2009285530A1 (en) 2008-08-29 2009-08-28 High-throughput and non-invasive method to vitrify porcine embryos
BRPI0918262A BRPI0918262A2 (pt) 2008-08-29 2009-08-28 método de alta produção e não-invasivo para vitrificar embriões suínos
EP09810683.4A EP2331677A4 (en) 2008-08-29 2009-08-28 NON-INVASIVE HIGH-PURPOSE METHOD FOR VITRIFICATION OF SWINE EMBRYOS

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US19051508P 2008-08-29 2008-08-29
US61/190,515 2008-08-29

Publications (1)

Publication Number Publication Date
WO2010025404A1 true WO2010025404A1 (en) 2010-03-04

Family

ID=41721960

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/055424 Ceased WO2010025404A1 (en) 2008-08-29 2009-08-28 High-throughput and non-invasive method to vitrify porcine embryos

Country Status (9)

Country Link
US (1) US8852078B2 (https=)
EP (1) EP2331677A4 (https=)
JP (1) JP2012501187A (https=)
CN (1) CN102197130A (https=)
AU (1) AU2009285530A1 (https=)
BR (1) BRPI0918262A2 (https=)
CA (1) CA2741754A1 (https=)
CL (1) CL2011000438A1 (https=)
WO (1) WO2010025404A1 (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9433195B2 (en) 2012-06-06 2016-09-06 Inguran, Llc Methods for increasing genetic progress in a line or breed of swine using sex-selected sperm cells
US9888990B2 (en) 2012-06-06 2018-02-13 Inguran, Llc Methods for use of sex sorted semen to improve genetic management in swine

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10531657B2 (en) 2015-12-07 2020-01-14 Coopersurgical, Inc. Low temperature specimen carriers and related methods
US11856947B2 (en) 2020-02-17 2024-01-02 Cook Medical Technologies Llc System for automated permeation of a biological material and method of using same

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6503698B1 (en) * 2000-06-16 2003-01-07 The United States Of America As Represented By The Secretary Of Agriculture Cryopreservation of swine embryos

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995005075A1 (en) * 1993-08-13 1995-02-23 Bresatec Limited Cryopreservation of porcine embryos
US6485959B1 (en) * 1998-10-07 2002-11-26 Cedars Sinai Medical Center Cell preconditioning and cryopresevation medium
US20040161735A1 (en) * 1998-11-24 2004-08-19 The University Of Queensland Cryopreservation of oocytes and embryos and methods for producing animals involving the same
US20020045156A1 (en) * 2000-05-16 2002-04-18 Mehmet Toner Microinjection of cryoprotectants for preservation of cells
CN1199688C (zh) * 2003-06-05 2005-05-04 于靖波 促卵巢功能细胞生长素及其制备方法

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6503698B1 (en) * 2000-06-16 2003-01-07 The United States Of America As Represented By The Secretary Of Agriculture Cryopreservation of swine embryos

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9433195B2 (en) 2012-06-06 2016-09-06 Inguran, Llc Methods for increasing genetic progress in a line or breed of swine using sex-selected sperm cells
US9888990B2 (en) 2012-06-06 2018-02-13 Inguran, Llc Methods for use of sex sorted semen to improve genetic management in swine
US10188082B2 (en) 2012-06-06 2019-01-29 Inguran, Llc Methods for increasing genetic progress in a line or breed of swine using sex-selected sperm cells
US10219883B2 (en) 2012-06-06 2019-03-05 Inguran, Llc Methods for use of sex sorted semen to improve genetic management in swine
US10543070B2 (en) 2012-06-06 2020-01-28 Inguran, Llc Methods for use of sex sorted semen to improve genetic management in swine
US10542734B2 (en) 2012-06-06 2020-01-28 Inguran, Llc Methods for increasing genetic progress in a line or breed of swine using sex-selected sperm cells
US11653635B2 (en) 2012-06-06 2023-05-23 Inguran, Llc Methods for increasing genetic progress in a line or breed of swine using sex-selected sperm cells
US12121547B2 (en) 2012-06-06 2024-10-22 Inguran, Llc Methods for use of sex sorted semen to improve genetic management in swine

Also Published As

Publication number Publication date
CA2741754A1 (en) 2010-03-04
CN102197130A (zh) 2011-09-21
EP2331677A4 (en) 2013-07-24
BRPI0918262A2 (pt) 2016-07-26
US8852078B2 (en) 2014-10-07
EP2331677A1 (en) 2011-06-15
JP2012501187A (ja) 2012-01-19
US20110213198A1 (en) 2011-09-01
CL2011000438A1 (es) 2011-07-01
AU2009285530A1 (en) 2010-03-04

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