WO2010014622A3 - In vitro model for modulating the blood brain barrier and methods of screening - Google Patents

In vitro model for modulating the blood brain barrier and methods of screening Download PDF

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Publication number
WO2010014622A3
WO2010014622A3 PCT/US2009/051978 US2009051978W WO2010014622A3 WO 2010014622 A3 WO2010014622 A3 WO 2010014622A3 US 2009051978 W US2009051978 W US 2009051978W WO 2010014622 A3 WO2010014622 A3 WO 2010014622A3
Authority
WO
WIPO (PCT)
Prior art keywords
brain barrier
methods
screening
blood brain
modulating
Prior art date
Application number
PCT/US2009/051978
Other languages
French (fr)
Other versions
WO2010014622A2 (en
Inventor
Elisabetta Dejana
Stefan Liebner
Original Assignee
Fondazione Istituto Firc Di Oncologica Molecolare (Ifom)
Geraghty, Erin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fondazione Istituto Firc Di Oncologica Molecolare (Ifom), Geraghty, Erin filed Critical Fondazione Istituto Firc Di Oncologica Molecolare (Ifom)
Publication of WO2010014622A2 publication Critical patent/WO2010014622A2/en
Publication of WO2010014622A3 publication Critical patent/WO2010014622A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5064—Endothelial cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/069—Vascular Endothelial cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C12N2503/02—Drug screening
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin

Abstract

An in vitro blood brain barrier (BBB) model is provided as well as in vitro methods for screening molecules for the ability to pass through the BBB or the ability to tighten the endothelial cell junctions in the BBB.
PCT/US2009/051978 2008-07-28 2009-07-28 In vitro model for modulating the blood brain barrier and methods of screening WO2010014622A2 (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US8422508P 2008-07-28 2008-07-28
US61/084,225 2008-07-28
US8449208P 2008-07-29 2008-07-29
US61/084,492 2008-07-29
US19311608P 2008-10-29 2008-10-29
US61/193,116 2008-10-29

Publications (2)

Publication Number Publication Date
WO2010014622A2 WO2010014622A2 (en) 2010-02-04
WO2010014622A3 true WO2010014622A3 (en) 2010-05-06

Family

ID=41610935

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/051978 WO2010014622A2 (en) 2008-07-28 2009-07-28 In vitro model for modulating the blood brain barrier and methods of screening

Country Status (1)

Country Link
WO (1) WO2010014622A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2770223T3 (en) 2016-01-29 2020-07-01 Hoffmann La Roche Electrochemiluminescence procedure and apparatus for detecting an analyte in a liquid sample
US10143187B2 (en) 2017-02-17 2018-12-04 Denali Therapeutics Inc. Transferrin receptor transgenic models
JP2020508049A (en) 2017-02-17 2020-03-19 デナリ セラピューティクス インコーポレイテッドＤｅｎａｌｉ Ｔｈｅｒａｐｅｕｔｉｃｓ Ｉｎｃ． Engineered transferrin receptor binding polypeptide
US10457717B2 (en) 2017-02-17 2019-10-29 Denali Therapeutics Inc. Engineered polypeptides
CN114107386B (en) * 2021-11-29 2024-02-02 中国人民解放军军事科学院军事医学研究院 Method for preparing mouse model with blood brain barrier defect

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001049709A1 (en) * 2000-01-03 2001-07-12 Ramot University Authority For Applied Research & Industrial Development Ltd. Glycogen synthase kinase-3 inhibitors
US6403325B1 (en) * 1997-06-30 2002-06-11 The Brigham And Women's Hospital, Inc. Methods and compounds for treating alzheimer's disease
WO2005063966A2 (en) * 2003-12-23 2005-07-14 Axaron Bioscience Ag Method for in vitro differentiation of neuronal stem cells or cells derived from neuronal stem cells

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6403325B1 (en) * 1997-06-30 2002-06-11 The Brigham And Women's Hospital, Inc. Methods and compounds for treating alzheimer's disease
WO2001049709A1 (en) * 2000-01-03 2001-07-12 Ramot University Authority For Applied Research & Industrial Development Ltd. Glycogen synthase kinase-3 inhibitors
WO2005063966A2 (en) * 2003-12-23 2005-07-14 Axaron Bioscience Ag Method for in vitro differentiation of neuronal stem cells or cells derived from neuronal stem cells

Also Published As

Publication number Publication date
WO2010014622A2 (en) 2010-02-04

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