WO2010013442A1 - Light-responsive nucleotide molecule, and method for production of light-responsive modified nucleic acid by using the light-responsive nucleotide molecule - Google Patents

Light-responsive nucleotide molecule, and method for production of light-responsive modified nucleic acid by using the light-responsive nucleotide molecule Download PDF

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Publication number
WO2010013442A1
WO2010013442A1 PCT/JP2009/003540 JP2009003540W WO2010013442A1 WO 2010013442 A1 WO2010013442 A1 WO 2010013442A1 JP 2009003540 W JP2009003540 W JP 2009003540W WO 2010013442 A1 WO2010013442 A1 WO 2010013442A1
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WO
WIPO (PCT)
Prior art keywords
light
responsive
nucleotide molecule
nucleic acid
modified nucleic
Prior art date
Application number
PCT/JP2009/003540
Other languages
French (fr)
Japanese (ja)
Inventor
藤本健造
荻野雅之
新田ゆかり
Original Assignee
国立大学法人北陸先端科学技術大学院大学
エスシーワールド株式会社
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 国立大学法人北陸先端科学技術大学院大学, エスシーワールド株式会社 filed Critical 国立大学法人北陸先端科学技術大学院大学
Priority to JP2010522616A priority Critical patent/JP5425780B2/en
Publication of WO2010013442A1 publication Critical patent/WO2010013442A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Abstract

Disclosed are: a method for producing a long-chain light-responsive modified nucleic acid without the need of employing any DNA synthesizer; and a light-responsive artificial nucleotide molecule for use in the method. Specifically disclosed are: a light-responsive α-dideoxyribonucleotide   having a light-responsive α- dideoxyribonucleoside as a nucleoside moiety; and a method for producing a light-responsive modified nucleic acid by introducing the light-responsive α-dideoxyribonucleotide into a nucleic acid.
PCT/JP2009/003540 2008-07-30 2009-07-28 Light-responsive nucleotide molecule, and method for production of light-responsive modified nucleic acid by using the light-responsive nucleotide molecule WO2010013442A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2010522616A JP5425780B2 (en) 2008-07-30 2009-07-28 Photoresponsive nucleotide molecule and method for producing photoresponsive modified nucleic acids using the photoresponsive nucleotide molecule

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2008195739 2008-07-30
JP2008-195739 2008-07-30

Publications (1)

Publication Number Publication Date
WO2010013442A1 true WO2010013442A1 (en) 2010-02-04

Family

ID=41610156

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2009/003540 WO2010013442A1 (en) 2008-07-30 2009-07-28 Light-responsive nucleotide molecule, and method for production of light-responsive modified nucleic acid by using the light-responsive nucleotide molecule

Country Status (2)

Country Link
JP (1) JP5425780B2 (en)
WO (1) WO2010013442A1 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63241356A (en) * 1986-11-20 1988-10-06 Sekisui Chem Co Ltd Determination of base sequence for polynucleotide and/or oligonucleotide and analyzer using the same
JP2005350386A (en) * 2004-06-09 2005-12-22 Japan Science & Technology Agency Deoxyuridine derivative and photoresponsive nucleotide

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63241356A (en) * 1986-11-20 1988-10-06 Sekisui Chem Co Ltd Determination of base sequence for polynucleotide and/or oligonucleotide and analyzer using the same
JP2005350386A (en) * 2004-06-09 2005-12-22 Japan Science & Technology Agency Deoxyuridine derivative and photoresponsive nucleotide

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
"79th Annual Meeting on Chemical Society of Japan in Spring (2001) Koen Yokoshu II", 2001, article KENZO FUJIMOTO: "Hikari o Mochiita Idenshi Sosaho (9) Hikari Renketsusei Shushoku Enki no Kosoteki Donyu", pages: 972 *
"85th Annual Meeting on Chemical Society of Japan in Spring (2005) Koen Yokoshu II", 2005, article MASAYUKI OGINO ET AL.: "3' Mattangawa kara no a-5-cyanovinyldeoxy-uridine o Mochiita DNA Kagyakuteki Hikari Renketsu Hanno", pages: 1369 *
"89th Annual Meeting on Chemical Society of Japan in Spring (2009) Koen Yokoshu I", 13 March 2009, article YUKARI NITTA ET AL.: "Hikari Otosei a-Dideoxyuridine Yudotai o Donyu shita Cloning Vector no Kochiku", pages: 632 *
DMITRY G. SEMIZAROV ET AL.: "Stereoisomers of Deoxynucleoside 5'-Triphosphates as Substrates for Template-dependent and -independent DNA Polymerases", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 272, no. 14, 1997, pages 9556 - 9560 *
MASAYUKI OGINO ET AL.: "Template-Directed DNA Photoligation via a-5-Cyanovinyldeoxyuridine", ORGANIC LETTERS, vol. 7, no. 14, 2005, pages 2853 - 2856 *

Also Published As

Publication number Publication date
JP5425780B2 (en) 2014-02-26
JPWO2010013442A1 (en) 2012-01-05

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