WO2010006166A3 - Bead manipulation techniques - Google Patents
Bead manipulation techniques Download PDFInfo
- Publication number
- WO2010006166A3 WO2010006166A3 PCT/US2009/050101 US2009050101W WO2010006166A3 WO 2010006166 A3 WO2010006166 A3 WO 2010006166A3 US 2009050101 W US2009050101 W US 2009050101W WO 2010006166 A3 WO2010006166 A3 WO 2010006166A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- droplet
- magnetically responsive
- responsive beads
- beads
- redistributing
- Prior art date
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C5/00—Separating dispersed particles from liquids by electrostatic effect
- B03C5/02—Separators
- B03C5/022—Non-uniform field separators
- B03C5/028—Non-uniform field separators using travelling electric fields, i.e. travelling wave dielectrophoresis [TWD]
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C5/00—Separating dispersed particles from liquids by electrostatic effect
- B03C5/005—Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength
Abstract
The invention provides a method of redistributing magnetically responsive beads in a droplet. The method may include providing a droplet including magnetically responsive beads. The droplet may be provided within a region of a magnetic field having sufficient strength to attract the magnetically responsive beads to an edge of the droplet or towards an edge of the droplet, or otherwise regionalize or aggregate beads within the droplet. The method may also include conducting on a droplet operations surface one or more droplet operations using the droplet without removing the magnetically responsive beads from the region of the magnetic field. The droplet operations may in some cases be electrode -mediated. The droplet operations may redistribute and/or circulate the magnetically responsive beads within the droplet. In some cases, the droplet may include a sample droplet may include a target analyte. The redistributing of the magnetically responsive beads may cause target analyte to bind to the magnetically responsive beads. In some cases, the droplet may include unbound substances in a wash buffer. The redistributing of the magnetically responsive beads causes unbound substances to be freed from interstices of an aggregated set or subset of the magnetically responsive beads.
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US12/985,409 US8637317B2 (en) | 2006-04-18 | 2011-01-06 | Method of washing beads |
US14/163,442 US20140193807A1 (en) | 2006-04-18 | 2014-01-24 | Bead manipulation techniques |
US14/491,231 US9050606B2 (en) | 2006-04-13 | 2014-09-19 | Bead manipulation techniques |
US14/697,058 US9205433B2 (en) | 2006-04-13 | 2015-04-27 | Bead manipulation techniques |
US14/928,096 US9358551B2 (en) | 2006-04-13 | 2015-10-30 | Bead manipulation techniques |
US15/165,883 US20160271623A1 (en) | 2006-04-13 | 2016-05-26 | Bead Manipulation Techniques |
Applications Claiming Priority (12)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US7934608P | 2008-07-09 | 2008-07-09 | |
US61/079,346 | 2008-07-09 | ||
US8073108P | 2008-07-15 | 2008-07-15 | |
US61/080,731 | 2008-07-15 | ||
US10330208P | 2008-10-07 | 2008-10-07 | |
US61/103,302 | 2008-10-07 | ||
US10899708P | 2008-10-28 | 2008-10-28 | |
US61/108,997 | 2008-10-28 | ||
US12279108P | 2008-12-16 | 2008-12-16 | |
US61/122,791 | 2008-12-16 | ||
US14980809P | 2009-02-04 | 2009-02-04 | |
US61/149,808 | 2009-02-04 |
Related Child Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US11/639,531 Continuation US8613889B2 (en) | 2006-04-13 | 2006-12-15 | Droplet-based washing |
US12/985,409 Continuation US8637317B2 (en) | 2006-04-13 | 2011-01-06 | Method of washing beads |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2010006166A2 WO2010006166A2 (en) | 2010-01-14 |
WO2010006166A3 true WO2010006166A3 (en) | 2010-04-29 |
Family
ID=41507733
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2009/050101 WO2010006166A2 (en) | 2006-04-13 | 2009-07-09 | Bead manipulation techniques |
Country Status (1)
Country | Link |
---|---|
WO (1) | WO2010006166A2 (en) |
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JP5897780B2 (en) | 2005-01-28 | 2016-03-30 | デューク ユニバーシティ | Apparatus and method for operating droplets on a printed circuit board |
US20140193807A1 (en) | 2006-04-18 | 2014-07-10 | Advanced Liquid Logic, Inc. | Bead manipulation techniques |
US10078078B2 (en) | 2006-04-18 | 2018-09-18 | Advanced Liquid Logic, Inc. | Bead incubation and washing on a droplet actuator |
WO2009140671A2 (en) | 2008-05-16 | 2009-11-19 | Advanced Liquid Logic, Inc. | Droplet actuator devices and methods for manipulating beads |
US8809068B2 (en) | 2006-04-18 | 2014-08-19 | Advanced Liquid Logic, Inc. | Manipulation of beads in droplets and methods for manipulating droplets |
US8658111B2 (en) | 2006-04-18 | 2014-02-25 | Advanced Liquid Logic, Inc. | Droplet actuators, modified fluids and methods |
US8637324B2 (en) | 2006-04-18 | 2014-01-28 | Advanced Liquid Logic, Inc. | Bead incubation and washing on a droplet actuator |
US7439014B2 (en) | 2006-04-18 | 2008-10-21 | Advanced Liquid Logic, Inc. | Droplet-based surface modification and washing |
WO2009111769A2 (en) | 2008-03-07 | 2009-09-11 | Advanced Liquid Logic, Inc. | Reagent and sample preparation and loading on a fluidic device |
US8685344B2 (en) | 2007-01-22 | 2014-04-01 | Advanced Liquid Logic, Inc. | Surface assisted fluid loading and droplet dispensing |
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WO2009032863A2 (en) | 2007-09-04 | 2009-03-12 | Advanced Liquid Logic, Inc. | Droplet actuator with improved top substrate |
KR20100100974A (en) | 2007-12-23 | 2010-09-15 | 어드밴스드 리퀴드 로직, 아이엔씨. | Droplet actuator configurations and methods of conducting droplet operations |
US8852952B2 (en) | 2008-05-03 | 2014-10-07 | Advanced Liquid Logic, Inc. | Method of loading a droplet actuator |
US8877512B2 (en) | 2009-01-23 | 2014-11-04 | Advanced Liquid Logic, Inc. | Bubble formation techniques using physical or chemical features to retain a gas bubble within a droplet actuator |
US8926065B2 (en) | 2009-08-14 | 2015-01-06 | Advanced Liquid Logic, Inc. | Droplet actuator devices and methods |
US9091649B2 (en) | 2009-11-06 | 2015-07-28 | Advanced Liquid Logic, Inc. | Integrated droplet actuator for gel; electrophoresis and molecular analysis |
EP2516669B1 (en) | 2009-12-21 | 2016-10-12 | Advanced Liquid Logic, Inc. | Enzyme assays on a droplet actuator |
WO2011126892A2 (en) | 2010-03-30 | 2011-10-13 | Advanced Liquid Logic, Inc. | Droplet operations platform |
EP2567213B1 (en) | 2010-05-05 | 2018-01-24 | The Governing Council of the Universtiy of Toronto | Method of processing dried samples using digital microfluidic device |
EP2588322B1 (en) | 2010-06-30 | 2015-06-17 | Advanced Liquid Logic, Inc. | Droplet actuator assemblies and methods of making same |
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EP2707724A4 (en) | 2011-05-10 | 2015-01-21 | Advanced Liquid Logic Inc | Enzyme concentration and assays |
US8901043B2 (en) | 2011-07-06 | 2014-12-02 | Advanced Liquid Logic, Inc. | Systems for and methods of hybrid pyrosequencing |
BR112014000257A2 (en) | 2011-07-06 | 2017-03-01 | Advanced Liquid Logic Inc | reagent storage in a drop actuator |
US9513253B2 (en) | 2011-07-11 | 2016-12-06 | Advanced Liquid Logic, Inc. | Droplet actuators and techniques for droplet-based enzymatic assays |
WO2013016413A2 (en) | 2011-07-25 | 2013-01-31 | Advanced Liquid Logic Inc | Droplet actuator apparatus and system |
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WO2013070627A2 (en) | 2011-11-07 | 2013-05-16 | Illumina, Inc. | Integrated sequencing apparatuses and methods of use |
WO2013078216A1 (en) | 2011-11-21 | 2013-05-30 | Advanced Liquid Logic Inc | Glucose-6-phosphate dehydrogenase assays |
US9223317B2 (en) | 2012-06-14 | 2015-12-29 | Advanced Liquid Logic, Inc. | Droplet actuators that include molecular barrier coatings |
IN2015DN00359A (en) | 2012-06-27 | 2015-06-12 | Advanced Liquid Logic Inc | |
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US10563240B2 (en) | 2013-03-14 | 2020-02-18 | Life Technologies Corporation | High efficiency, small volume nucleic acid synthesis |
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CA2970477C (en) * | 2014-12-09 | 2022-03-15 | Life Technologies Corporation | High efficiency, small volume nucleic acid synthesis |
CN208562324U (en) | 2015-06-05 | 2019-03-01 | 米罗库鲁斯公司 | Digital microcurrent-controlled (DMF) device of air matrix |
US10464067B2 (en) | 2015-06-05 | 2019-11-05 | Miroculus Inc. | Air-matrix digital microfluidics apparatuses and methods for limiting evaporation and surface fouling |
CA3034064A1 (en) | 2016-08-22 | 2018-03-01 | Miroculus Inc. | Feedback system for parallel droplet control in a digital microfluidic device |
JP2020515815A (en) | 2016-12-28 | 2020-05-28 | ミロキュラス インコーポレイテッド | Digital microfluidic device and method |
WO2018187476A1 (en) | 2017-04-04 | 2018-10-11 | Miroculus Inc. | Digital microfluidic apparatuses and methods for manipulating and processing encapsulated droplets |
WO2019023133A1 (en) | 2017-07-24 | 2019-01-31 | Miroculus Inc. | Digital microfluidics systems and methods with integrated plasma collection device |
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US11857961B2 (en) | 2022-01-12 | 2024-01-02 | Miroculus Inc. | Sequencing by synthesis using mechanical compression |
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US20070275415A1 (en) * | 2006-04-18 | 2007-11-29 | Vijay Srinivasan | Droplet-based affinity assays |
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2009
- 2009-07-09 WO PCT/US2009/050101 patent/WO2010006166A2/en active Application Filing
Patent Citations (4)
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US20050056569A1 (en) * | 2003-07-29 | 2005-03-17 | National Tsing Hua University | Electrowetting electrode device with electromagnetic field for actuation of magnetic-bead biochemical detection system |
EP1844852A1 (en) * | 2006-04-13 | 2007-10-17 | Samsung Electronics Co., Ltd. | Apparatus and method for printing biomolecular droplet on substrate |
US20070243634A1 (en) * | 2006-04-18 | 2007-10-18 | Pamula Vamsee K | Droplet-based surface modification and washing |
US20070275415A1 (en) * | 2006-04-18 | 2007-11-29 | Vijay Srinivasan | Droplet-based affinity assays |
Also Published As
Publication number | Publication date |
---|---|
WO2010006166A2 (en) | 2010-01-14 |
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